pycom13g07430

SAM domain (Sterile alpha motif)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001403
Physical Location & Seq
Forward (+)
4932516 .. 4935966
3451 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g07430.1

Sequence Viewer

Length: 1017 bp
ATGTCAGGGAATTCGAGGGGCCGAGTCACCATAACGCTTGGGCGCGGTGGTCAGGTGGTGAAGAGGTCTGTTTCGGGGTCTTCATTAGCTGATCATATACCTGCGGCTGGGGCCAAGCGTTCTGTTAGAGATAGGTTAGGAGGTAATGTAGACGGTGCTTTGTCACACGGAAGCCTACACAGCAATAAACGCCAGCGAGGAGACAATGGTAGGTCAAGTATGAGTTCCAATGGTTATGATGATGCACGCATTGGTAAAGGTGATCTTCGAATTAAACTCATGCAAAAAAATGCGTTTAGACGAGCTCAGAGTGATGATGGTCATAGGCACGGGGACCTCCGTGAAAAGCTGTCAAAGGCAGTTCAGCGCCCTGTTACTGGCCATGACCCATGTCAGCACCATTCCGAAATAAAAGAGACTGGCTTTATGGGCCGAATTCCTTCTTCAAGGGCAGATGATTTACCTCGTGTGGATTCATTAAGAAATTCTTATTCCCCTTGGACACTGGACCATATAAGACGAAGATCCCCAGATAGAGTTGTAGTCACTTCGAGGGGTCTCTCCCCTCCGAGGAATCCCAAAGAATTGCAGAGAAGGCCAGTAAGTAGGGCATTTGATGATGTCAGATCGGTTCCATATATGAGCAAAGATGTTCTAAATGCTACAAGGCCTACAAGTACAACTCCTTTCATGTCCAGTTCTCCATTGCCTCCTGTATCTGCGAAGCCTGTGGCCCCACCCCCACCTCATTTGAGCCAAATTCCTCCTCTGAGCAGCATTGCACAGAAAAGTTCATATGTGAGTGATGAACAACAGACTGTAGATGGCTTGCTACATGCGCTGGGGTTGGGAAAATATGCCATTATTTTTAAGGCTGAGGAAATTGATATGACTGCATTGAAGCAGATGGGAGAATATGACCTCAAAGAGCTGGGAATACCTATGGGACCTAGGAAGAAGCTTCTTCTTGCCCTCCTGCCTCGTTCCAAGCGGCCATCAACTACAAATATACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_1

Protein Analysis

339

Amino Acids

37.07

Weight (kDa)

10.59

Isoelectric Point (pI)

64.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_1 PF00536 271 - 325 3e-12 SAM domain (Sterile alpha motif)
SAM_2 PF07647 272 - 324 2.9e-07 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 109
AccI GTMKAC 1 cut(s) 150
AccII CGCG 1 cut(s) 45
AciI CCGC 3 cut(s) 45, 104, 991
AclWI GGATC 1 cut(s) 519
AcoI YGGCCR 2 cut(s) 379, 992
AcsI RAATTY 4 cut(s) 10, 435, 484, 759
AfaI GTAC 1 cut(s) 679
AfiI CCNNNNNNNGG 3 cut(s) 107, 377, 570
AgsI TTSAA 2 cut(s) 447, 901
AluBI AGCT 5 cut(s) 89, 305, 349, 931, 961
AluI AGCT 5 cut(s) 89, 305, 349, 931, 961
Alw21I GWGCWC 1 cut(s) 307
Alw26I GTCTC 3 cut(s) 195, 410, 563
AlwI GGATC 1 cut(s) 519
AoxI GGCC 8 cut(s) 19, 111, 379, 430, 596, 668, 732, 992
ApeKI GCWGC 1 cut(s) 774
ApoI RAATTY 4 cut(s) 10, 435, 484, 759
Asp700I GAANNNNTTC 1 cut(s) 439
AspA2I CCTAGG 1 cut(s) 950
AspLEI GCGC 3 cut(s) 45, 369, 841
AspS9I GGNCC 7 cut(s) 19, 111, 334, 430, 508, 733, 947
AsuHPI GGTGA 3 cut(s) 19, 70, 272
AsuII TTCGAA 1 cut(s) 268
AvaII GGWCC 3 cut(s) 334, 508, 947
AvrII CCTAGG 1 cut(s) 950
BalI TGGCCA 1 cut(s) 381
BanII GRGCYC 1 cut(s) 307
BauI CACGAG 1 cut(s) 465
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 307
BbvCI CCTCAGC 1 cut(s) 876
BbvI GCAGC 1 cut(s) 786
BccI CCATC 4 cut(s) 311, 818, 901, 1003
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 3 cut(s) 195, 410, 563
BfaI CTAG 1 cut(s) 951
BfmI CTRYAG 1 cut(s) 819
BfoI RGCGCY 1 cut(s) 370
BfuAI ACCTGC 1 cut(s) 109
BisI GCNGC 3 cut(s) 105, 775, 992
BlnI CCTAGG 1 cut(s) 950
BlsI GCNGC 3 cut(s) 106, 776, 993
Bme18I GGWCC 3 cut(s) 334, 508, 947
BmgT120I GGNCC 7 cut(s) 19, 111, 334, 430, 508, 733, 947
BmiI GGNNCC 6 cut(s) 20, 112, 335, 633, 735, 948
BmsI GCATC 1 cut(s) 232
BoxI GACNNNNGTC 1 cut(s) 390
BpiI GAAGAC 1 cut(s) 72
Bpu10I CCTNAGC 1 cut(s) 876
Bpu14I TTCGAA 1 cut(s) 268
BsaI GGTCTC 1 cut(s) 563
BsaJI CCNNGG 3 cut(s) 497, 569, 950
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 3 cut(s) 107, 377, 570
Bse1I ACTGG 5 cut(s) 382, 424, 510, 599, 696
Bse3DI GCAATG 2 cut(s) 704, 777
BseDI CCNNGG 3 cut(s) 497, 569, 950
BseLI CCNNNNNNNGG 3 cut(s) 107, 377, 570
BseMI GCAATG 2 cut(s) 704, 777
BseMII CTCAG 3 cut(s) 320, 761, 867
BseNI ACTGG 5 cut(s) 382, 424, 510, 599, 696
BseRI GAGGAG 2 cut(s) 213, 756
BseXI GCAGC 1 cut(s) 786
BseYI CCCAGC 3 cut(s) 107, 841, 931
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 8 cut(s) 21, 113, 381, 432, 598, 670, 734, 994
BsiHKAI GWGCWC 1 cut(s) 307
BslFI GGGAC 2 cut(s) 347, 960
BslI CCNNNNNNNGG 3 cut(s) 107, 377, 570
BsmAI GTCTC 3 cut(s) 195, 410, 563
BsmFI GGGAC 2 cut(s) 347, 960
BsnI GGCC 8 cut(s) 21, 113, 381, 432, 598, 670, 734, 994
Bso31I GGTCTC 1 cut(s) 563
Bsp119I TTCGAA 1 cut(s) 268
Bsp1286I GDGCHC 1 cut(s) 307
Bsp143I GATC 4 cut(s) 91, 262, 524, 626
BspACI CCGC 3 cut(s) 45, 104, 991
BspANI GGCC 8 cut(s) 21, 113, 381, 432, 598, 670, 734, 994
BspCNI CTCAG 3 cut(s) 319, 762, 868
BspFNI CGCG 1 cut(s) 45
BspLI GGNNCC 6 cut(s) 20, 112, 335, 633, 735, 948
BspMI ACCTGC 1 cut(s) 109
BspPI GGATC 1 cut(s) 519
BspT104I TTCGAA 1 cut(s) 268
BspTNI GGTCTC 1 cut(s) 563
BsrDI GCAATG 2 cut(s) 704, 777
BsrI ACTGG 5 cut(s) 382, 424, 510, 599, 696
BssECI CCNNGG 3 cut(s) 497, 569, 950
BssMI GATC 4 cut(s) 91, 262, 524, 626
BssSI CACGAG 1 cut(s) 465
BssT1I CCWWGG 2 cut(s) 497, 950
Bst2BI CACGAG 1 cut(s) 465
Bst4CI ACNGT 2 cut(s) 155, 820
Bst6I CTCTTC 1 cut(s) 56
BstBI TTCGAA 1 cut(s) 268
BstC8I GCNNGC 3 cut(s) 194, 247, 830
BstDEI CTNAG 3 cut(s) 306, 770, 876
BstFNI CGCG 1 cut(s) 45
BstH2I RGCGCY 1 cut(s) 370
BstHHI GCGC 3 cut(s) 45, 369, 841
BstKTI GATC 4 cut(s) 94, 265, 527, 629
BstMAI GTCTC 3 cut(s) 195, 410, 563
BstMBI GATC 4 cut(s) 91, 262, 524, 626
BstMWI GCNNNNNNNGC 6 cut(s) 110, 180, 189, 429, 595, 838
BstNSI RCATGY 1 cut(s) 839
BstPAI GACNNNNGTC 1 cut(s) 390
BstSFI CTRYAG 1 cut(s) 819
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 1 cut(s) 786
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 1 cut(s) 524
BstYI RGATCY 1 cut(s) 524
BsuRI GGCC 8 cut(s) 21, 113, 381, 432, 598, 670, 734, 994
BtsIMutI CAGTG 1 cut(s) 503
BveI ACCTGC 1 cut(s) 109
Cac8I GCNNGC 3 cut(s) 194, 247, 830
CfoI GCGC 3 cut(s) 45, 369, 841
Cfr13I GGNCC 7 cut(s) 19, 111, 334, 430, 508, 733, 947
Csp6I GTAC 1 cut(s) 678
CspCI CAANNNNNGTGG 2 cut(s) 732, 767
CviAII CATG 5 cut(s) 280, 383, 390, 691, 836
CviQI GTAC 1 cut(s) 678
DdeI CTNAG 3 cut(s) 306, 770, 876
DpnI GATC 4 cut(s) 93, 264, 526, 628
DpnII GATC 4 cut(s) 91, 262, 524, 626
EaeI YGGCCR 2 cut(s) 379, 992
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Ecl136II GAGCTC 1 cut(s) 305
Eco130I CCWWGG 2 cut(s) 497, 950
Eco147I AGGCCT 1 cut(s) 670
Eco24I GRGCYC 1 cut(s) 307
Eco31I GGTCTC 1 cut(s) 563
Eco47I GGWCC 3 cut(s) 334, 508, 947
Eco53kI GAGCTC 1 cut(s) 305
EcoICRI GAGCTC 1 cut(s) 305
EcoO109I RGGNCCY 2 cut(s) 334, 947
EcoRI GAATTC 2 cut(s) 10, 435
EcoT14I CCWWGG 2 cut(s) 497, 950
EcoT38I GRGCYC 1 cut(s) 307
ErhI CCWWGG 2 cut(s) 497, 950
FaeI CATG 5 cut(s) 283, 386, 393, 694, 839
FalI AAGNNNNNCTT 4 cut(s) 249, 281, 472, 504
FaqI GGGAC 2 cut(s) 347, 960
FatI CATG 5 cut(s) 279, 382, 389, 690, 835
FauNDI CATATG 1 cut(s) 796
FbaI TGATCA 1 cut(s) 91
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 3 cut(s) 105, 775, 992
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 3 cut(s) 105, 775, 992
FspBI CTAG 1 cut(s) 951
GlaI GCGC 3 cut(s) 44, 368, 840
GluI GCNGC 3 cut(s) 105, 775, 992
GsaI CCCAGC 3 cut(s) 111, 845, 935
HaeII RGCGCY 1 cut(s) 370
HaeIII GGCC 8 cut(s) 21, 113, 381, 432, 598, 670, 734, 994
HhaI GCGC 3 cut(s) 45, 369, 841
Hin1II CATG 5 cut(s) 283, 386, 393, 694, 839
Hin6I GCGC 3 cut(s) 43, 367, 839
HinP1I GCGC 3 cut(s) 43, 367, 839
HindIII AAGCTT 1 cut(s) 959
HinfI GANTC 3 cut(s) 24, 473, 574
HphI GGTGA 3 cut(s) 19, 70, 272
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 5 cut(s) 309, 406, 570, 626, 771
Hpy8I GTNNAC 1 cut(s) 151
HpyAV CCTTC 2 cut(s) 450, 588
HpyCH4III ACNGT 2 cut(s) 155, 820
HpyCH4IV ACGT 1 cut(s) 1012
HpyCH4V TGCA 5 cut(s) 245, 283, 589, 782, 896
HpyF10VI GCNNNNNNNGC 6 cut(s) 110, 180, 189, 429, 595, 838
HpyF3I CTNAG 3 cut(s) 306, 770, 876
HpySE526I ACGT 1 cut(s) 1012
Hsp92II CATG 5 cut(s) 283, 386, 393, 694, 839
HspAI GCGC 3 cut(s) 43, 367, 839
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 4 cut(s) 91, 262, 524, 626
Lsp1109I GCAGC 1 cut(s) 786
LweI GCATC 1 cut(s) 232
MaeI CTAG 1 cut(s) 951
MaeII ACGT 1 cut(s) 1012
MaeIII GTNAC 4 cut(s) 25, 162, 373, 544
MalI GATC 4 cut(s) 93, 264, 526, 628
MboI GATC 4 cut(s) 91, 262, 524, 626
MboII GAAGA 7 cut(s) 72, 73, 257, 435, 534, 956, 967
MflI RGATCY 1 cut(s) 524
MhlI GDGCHC 1 cut(s) 307
MlsI TGGCCA 1 cut(s) 381
MluCI AATT 7 cut(s) 10, 270, 435, 484, 584, 759, 882
MluNI TGGCCA 1 cut(s) 381
MlyI GAGTC 1 cut(s) 33
Mox20I TGGCCA 1 cut(s) 381
MroXI GAANNNNTTC 1 cut(s) 439
MscI TGGCCA 1 cut(s) 381
MseI TTAA 3 cut(s) 273, 479, 870
Msp20I TGGCCA 1 cut(s) 381
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 6 cut(s) 110, 180, 189, 429, 595, 838
NdeI CATATG 1 cut(s) 796
NdeII GATC 4 cut(s) 91, 262, 524, 626
NlaIII CATG 5 cut(s) 283, 386, 393, 694, 839
NlaIV GGNNCC 6 cut(s) 20, 112, 335, 633, 735, 948
NmeAIII GCCGAG 1 cut(s) 47
NmuCI GTSAC 3 cut(s) 25, 162, 544
NspI RCATGY 1 cut(s) 839
NspV TTCGAA 1 cut(s) 268
PceI AGGCCT 1 cut(s) 670
PdmI GAANNNNTTC 1 cut(s) 439
PfeI GAWTC 2 cut(s) 473, 574
PkrI GCNGC 3 cut(s) 106, 776, 993
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
PpuMI RGGWCCY 2 cut(s) 334, 947
PshAI GACNNNNGTC 1 cut(s) 390
Psp124BI GAGCTC 1 cut(s) 307
Psp5II RGGWCCY 2 cut(s) 334, 947
PspFI CCCAGC 3 cut(s) 107, 841, 931
PspN4I GGNNCC 6 cut(s) 20, 112, 335, 633, 735, 948
PspPI GGNCC 7 cut(s) 19, 111, 334, 430, 508, 733, 947
PspPPI RGGWCCY 2 cut(s) 334, 947
PsuI RGATCY 1 cut(s) 524
RsaI GTAC 1 cut(s) 679
RsaNI GTAC 1 cut(s) 678
SacI GAGCTC 1 cut(s) 307
SaqAI TTAA 3 cut(s) 273, 479, 870
SatI GCNGC 3 cut(s) 105, 775, 992
Sau3AI GATC 4 cut(s) 91, 262, 524, 626
Sau96I GGNCC 7 cut(s) 19, 111, 334, 430, 508, 733, 947
SchI GAGTC 1 cut(s) 33
SduI GDGCHC 1 cut(s) 307
SfaNI GCATC 1 cut(s) 232
SfcI CTRYAG 1 cut(s) 819
SfuI TTCGAA 1 cut(s) 268
SinI GGWCC 3 cut(s) 334, 508, 947
Sse9I AATT 7 cut(s) 10, 270, 435, 484, 584, 759, 882
SseBI AGGCCT 1 cut(s) 670
SsiI CCGC 3 cut(s) 45, 104, 991
SspMI CTAG 1 cut(s) 951
SstI GAGCTC 1 cut(s) 307
StuI AGGCCT 1 cut(s) 670
StyI CCWWGG 2 cut(s) 497, 950
TaaI ACNGT 2 cut(s) 155, 820
TaiI ACGT 1 cut(s) 1015
TaqI TCGA 3 cut(s) 14, 268, 551
TasI AATT 7 cut(s) 10, 270, 435, 484, 584, 759, 882
TatI WGTACW 1 cut(s) 677
TauI GCSGC 2 cut(s) 107, 994
TfiI GAWTC 2 cut(s) 473, 574
Tru1I TTAA 3 cut(s) 273, 479, 870
Tru9I TTAA 3 cut(s) 273, 479, 870
TscAI CASTG 1 cut(s) 510
TseFI GTSAC 3 cut(s) 25, 162, 544
TseI GCWGC 1 cut(s) 774
Tsp45I GTSAC 3 cut(s) 25, 162, 544
TspDTI ATGAA 5 cut(s) 72, 465, 679, 783, 822
TspGWI ACGGA 2 cut(s) 183, 329
TspRI CASTG 1 cut(s) 510
VpaK11BI GGWCC 3 cut(s) 334, 508, 947
XapI RAATTY 4 cut(s) 10, 435, 484, 759
XceI RCATGY 1 cut(s) 839
XmaJI CCTAGG 1 cut(s) 950
XmiI GTMKAC 1 cut(s) 150
XmnI GAANNNNTTC 1 cut(s) 439
XspI CTAG 1 cut(s) 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.