FvH4_5g26090

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001551
Physical Location & Seq
Forward (+)
17559268 .. 17561665
2398 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g26090.t1

Sequence Viewer

Length: 978 bp
ATGGGAGAGAAAGAAACGGTGTGTGTAACTGGAGCTGGAGGCTTTGTGGCATCTTGGGTCACCAAGTTGCTTCTCTCTGAGGGCTACAAAGTTCATGGCACTGCTAGAGACCCATGTAATGAGAAGAATGGTCATTTGAAGAAGTTGGTAAACGCTTCTGAGAATTTGCAACTGTTCAAGGCAGACTTGTTCGACTTTGAAGGTCTTTGTGCTGCCATTTCTGGGTGTTCTGGTGTTTTCCATGTTGCCTCCTCTGTTCCTTCCGACGAAAATTCCGTTTTCCATGATGAGGGAGAATTTATCGAACCTGCTCTGAGGGGCACACGGAATGTACTAGATGCATGTATCAAGGCAAAAGTAAAGAGGGTTGTGGTCGTGTCATCTGCTGGTGCTGTTGTATACAACCCGAAATGGCCCAAGGATCAACCTATGGATGAAGAGTGTCGGTCTGACCCTGAGTATTGCAAGAGAGCTAAGACAAACTTTGGCAACTATTGTCTTGCCAAGACAATGGCAGAAACTGAAGCTTTGGAGTATGCAAAGAAAAGTGAACCGAGAATCATAACAGTTTGCCCTTCCTTTGTTTTTGGTCCAATGCTGCAATCAACAGTAAATTCAAGTAACTTGGTGCTGATGTCTCTTCTGAAAGATGGACTCGAATCTGTTGAGAACCGTGTTCGTCCTGTTGTCGATGTGCGTGATGTAGCTGAAGCACTACTTCTGGCATATAAAAAGCCAGAAGCAGGAGGCAGATACATTTGTACATCTTATGTGATAAATGTGCAATCTTTGGTGAAGATGTTGAAGGGCTTGTATCCAAATTATAACTATCCCAAAAGAATCATTGAAGTAAATGAAGACATAAAGATCACTTCAGAGAAATTGCAGAAACTTGGTTGGAAGTATAGGCCATTAGAGGAGACGATTGTCGATGCTGTTAACGACTATACGAAGAAAGTGGAAATAAAGACTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
Epimerase

Protein Analysis

326

Amino Acids

36.07

Weight (kDa)

7.47

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 4 - 140 1.2e-08 NmrA-like family
Epimerase PF01370 7 - 251 2.7e-28 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 9 - 245 4e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 9 - 267 7e-15 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 11 - 136 1.1e-14 NAD(P)H-binding
NAD_binding_4 PF07993 77 - 214 8.7e-07 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 825
Acc36I ACCTGC 1 cut(s) 316
AccI GTMKAC 1 cut(s) 399
AclWI GGATC 1 cut(s) 429
AcsI RAATTY 4 cut(s) 163, 271, 296, 613
AcuI CTGAAG 3 cut(s) 543, 729, 858
AfaI GTAC 2 cut(s) 333, 763
AfiI CCNNNNNNNGG 3 cut(s) 222, 289, 743
AgsI TTSAA 6 cut(s) 139, 178, 200, 618, 805, 848
AluBI AGCT 5 cut(s) 35, 473, 527, 707, 975
AluI AGCT 5 cut(s) 35, 473, 527, 707, 975
Alw26I GTCTC 3 cut(s) 102, 642, 914
AlwI GGATC 1 cut(s) 429
AlwNI CAGNNNCTG 1 cut(s) 521
AoxI GGCC 2 cut(s) 413, 908
ApeKI GCWGC 2 cut(s) 212, 598
ApoI RAATTY 4 cut(s) 163, 271, 296, 613
AspS9I GGNCC 2 cut(s) 414, 590
AsuHPI GGTGA 2 cut(s) 52, 805
AvaII GGWCC 1 cut(s) 590
BaeGI GKGCMC 1 cut(s) 323
BaeI ACNNNNGTAYC 2 cut(s) 745, 778
BbsI GAAGAC 1 cut(s) 864
BbvI GCAGC 2 cut(s) 199, 585
BccI CCATC 1 cut(s) 644
BciVI GTATCC 1 cut(s) 825
BcoDI GTCTC 3 cut(s) 102, 642, 914
BfaI CTAG 4 cut(s) 105, 335, 972, 976
BfuAI ACCTGC 1 cut(s) 316
BfuI GTATCC 1 cut(s) 825
BisI GCNGC 2 cut(s) 213, 599
BlsI GCNGC 2 cut(s) 214, 600
Bme18I GGWCC 1 cut(s) 590
BmgT120I GGNCC 2 cut(s) 414, 590
BmsI GCATC 3 cut(s) 59, 328, 922
BoxI GACNNNNGTC 1 cut(s) 926
BpiI GAAGAC 1 cut(s) 864
BpmI CTGGAG 2 cut(s) 51, 57
BsaI GGTCTC 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 417
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 3 cut(s) 222, 289, 743
Bse1I ACTGG 1 cut(s) 34
BseDI CCNNGG 1 cut(s) 417
BseGI GGATG 1 cut(s) 439
BseLI CCNNNNNNNGG 3 cut(s) 222, 289, 743
BseMII CTCAG 4 cut(s) 69, 150, 305, 447
BseNI ACTGG 1 cut(s) 34
BseRI GAGGAG 2 cut(s) 241, 932
BseSI GKGCMC 1 cut(s) 323
BseXI GCAGC 2 cut(s) 199, 585
BshFI GGCC 2 cut(s) 415, 910
BslI CCNNNNNNNGG 3 cut(s) 222, 289, 743
BsmAI GTCTC 3 cut(s) 102, 642, 914
BsmBI CGTCTC 1 cut(s) 914
BsnI GGCC 2 cut(s) 415, 910
Bso31I GGTCTC 1 cut(s) 102
Bsp1286I GDGCHC 1 cut(s) 323
Bsp1407I TGTACA 1 cut(s) 761
Bsp143I GATC 2 cut(s) 421, 867
BspANI GGCC 2 cut(s) 415, 910
BspCNI CTCAG 4 cut(s) 70, 151, 306, 448
BspMI ACCTGC 1 cut(s) 316
BspPI GGATC 1 cut(s) 429
BspTNI GGTCTC 1 cut(s) 102
BsrGI TGTACA 1 cut(s) 761
BsrI ACTGG 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 417
BssMI GATC 2 cut(s) 421, 867
BssNAI GTATAC 1 cut(s) 400
BssT1I CCWWGG 1 cut(s) 417
Bst1107I GTATAC 1 cut(s) 400
Bst4CI ACNGT 5 cut(s) 19, 174, 568, 610, 674
Bst6I CTCTTC 2 cut(s) 432, 645
BstAUI TGTACA 1 cut(s) 761
BstDEI CTNAG 5 cut(s) 78, 159, 314, 456, 474
BstEII GGTNACC 1 cut(s) 58
BstF5I GGATG 1 cut(s) 439
BstKTI GATC 2 cut(s) 424, 870
BstMAI GTCTC 3 cut(s) 102, 642, 914
BstMBI GATC 2 cut(s) 421, 867
BstNSI RCATGY 1 cut(s) 345
BstPAI GACNNNNGTC 1 cut(s) 926
BstPI GGTNACC 1 cut(s) 58
BstSLI GKGCMC 1 cut(s) 323
BstV1I GCAGC 2 cut(s) 199, 585
BstV2I GAAGAC 1 cut(s) 864
BstXI CCANNNNNNTGG 1 cut(s) 511
BstZ17I GTATAC 1 cut(s) 400
BsuI GTATCC 1 cut(s) 825
BsuRI GGCC 2 cut(s) 415, 910
BtsCI GGATG 1 cut(s) 439
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 1 cut(s) 99
BveI ACCTGC 1 cut(s) 316
CaiI CAGNNNCTG 1 cut(s) 521
Cfr13I GGNCC 2 cut(s) 414, 590
Csp6I GTAC 2 cut(s) 332, 762
CviAII CATG 5 cut(s) 95, 114, 242, 284, 342
CviQI GTAC 2 cut(s) 332, 762
DdeI CTNAG 5 cut(s) 78, 159, 314, 456, 474
DpnI GATC 2 cut(s) 423, 869
DpnII GATC 2 cut(s) 421, 867
Eam1104I CTCTTC 2 cut(s) 432, 645
EarI CTCTTC 2 cut(s) 432, 645
Eco130I CCWWGG 1 cut(s) 417
Eco31I GGTCTC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 590
Eco57I CTGAAG 3 cut(s) 543, 729, 858
Eco91I GGTNACC 1 cut(s) 58
EcoO65I GGTNACC 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 417
EcoT22I ATGCAT 1 cut(s) 343
ErhI CCWWGG 1 cut(s) 417
Esp3I CGTCTC 1 cut(s) 914
FaeI CATG 5 cut(s) 98, 117, 245, 287, 345
FalI AAGNNNNNCTT 6 cut(s) 170, 202, 467, 499, 702, 734
FatI CATG 5 cut(s) 94, 113, 241, 283, 341
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 2 cut(s) 213, 599
FokI GGATG 1 cut(s) 446
Fsp4HI GCNGC 2 cut(s) 213, 599
FspBI CTAG 4 cut(s) 105, 335, 972, 976
GluI GCNGC 2 cut(s) 213, 599
GsuI CTGGAG 2 cut(s) 51, 57
HaeIII GGCC 2 cut(s) 415, 910
Hin1II CATG 5 cut(s) 98, 117, 245, 287, 345
HincII GTYRAC 1 cut(s) 940
HindII GTYRAC 1 cut(s) 940
HindIII AAGCTT 1 cut(s) 525
HinfI GANTC 4 cut(s) 558, 654, 659, 840
HpaI GTTAAC 1 cut(s) 940
HphI GGTGA 2 cut(s) 52, 805
Hpy166II GTNNAC 4 cut(s) 151, 400, 551, 940
Hpy188I TCNGA 7 cut(s) 79, 160, 265, 315, 451, 645, 877
Hpy8I GTNNAC 4 cut(s) 151, 400, 551, 940
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 4 cut(s) 194, 270, 585, 799
HpyCH4III ACNGT 5 cut(s) 19, 174, 568, 610, 674
HpyCH4V TGCA 7 cut(s) 169, 341, 465, 539, 601, 784, 886
HpyF3I CTNAG 5 cut(s) 78, 159, 314, 456, 474
Hsp92II CATG 5 cut(s) 98, 117, 245, 287, 345
KspAI GTTAAC 1 cut(s) 940
Kzo9I GATC 2 cut(s) 421, 867
LmnI GCTCC 1 cut(s) 32
Lsp1109I GCAGC 2 cut(s) 199, 585
LweI GCATC 3 cut(s) 59, 328, 922
MaeI CTAG 4 cut(s) 105, 335, 972, 976
MaeIII GTNAC 3 cut(s) 25, 58, 620
MalI GATC 2 cut(s) 423, 869
MboI GATC 2 cut(s) 421, 867
MboII GAAGA 7 cut(s) 136, 151, 449, 632, 808, 869, 964
MhlI GDGCHC 1 cut(s) 323
MluCI AATT 6 cut(s) 163, 271, 296, 613, 820, 881
MlyI GAGTC 1 cut(s) 648
MmeI TCCRAC 2 cut(s) 288, 878
MnlI CCTC 9 cut(s) 32, 73, 259, 262, 283, 309, 357, 740, 910
Mph1103I ATGCAT 1 cut(s) 343
MseI TTAA 1 cut(s) 939
NdeII GATC 2 cut(s) 421, 867
NlaIII CATG 5 cut(s) 98, 117, 245, 287, 345
NmuCI GTSAC 1 cut(s) 58
NsiI ATGCAT 1 cut(s) 343
NspI RCATGY 1 cut(s) 345
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PfeI GAWTC 3 cut(s) 558, 659, 840
PkrI GCNGC 2 cut(s) 214, 600
PleI GAGTC 1 cut(s) 648
PpsI GAGTC 1 cut(s) 648
PshAI GACNNNNGTC 1 cut(s) 926
PsiI TTATAA 1 cut(s) 825
PspEI GGTNACC 1 cut(s) 58
PspPI GGNCC 2 cut(s) 414, 590
PstNI CAGNNNCTG 1 cut(s) 521
RsaI GTAC 2 cut(s) 333, 763
RsaNI GTAC 2 cut(s) 332, 762
SaqAI TTAA 1 cut(s) 939
SatI GCNGC 2 cut(s) 213, 599
Sau3AI GATC 2 cut(s) 421, 867
Sau96I GGNCC 2 cut(s) 414, 590
SchI GAGTC 1 cut(s) 648
SduI GDGCHC 1 cut(s) 323
SetI ASST 8 cut(s) 37, 205, 310, 430, 475, 529, 709, 977
SfaNI GCATC 3 cut(s) 59, 328, 922
SinI GGWCC 1 cut(s) 590
Sse9I AATT 6 cut(s) 163, 271, 296, 613, 820, 881
SspMI CTAG 4 cut(s) 105, 335, 972, 976
StyI CCWWGG 1 cut(s) 417
TaaI ACNGT 5 cut(s) 19, 174, 568, 610, 674
TaqI TCGA 5 cut(s) 192, 303, 657, 690, 930
TaqII GACCGA 1 cut(s) 435
TasI AATT 6 cut(s) 163, 271, 296, 613, 820, 881
TatI WGTACW 2 cut(s) 331, 761
TfiI GAWTC 3 cut(s) 558, 659, 840
Tru1I TTAA 1 cut(s) 939
Tru9I TTAA 1 cut(s) 939
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 2 cut(s) 212, 598
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 3 cut(s) 83, 450, 870
TspGWI ACGGA 2 cut(s) 265, 340
TspRI CASTG 1 cut(s) 106
VpaK11BI GGWCC 1 cut(s) 590
XapI RAATTY 4 cut(s) 163, 271, 296, 613
XceI RCATGY 1 cut(s) 345
XmiI GTMKAC 1 cut(s) 399
XspI CTAG 4 cut(s) 105, 335, 972, 976
Zsp2I ATGCAT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.