RchiOBHm_Chr7g0216751

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001551
Physical Location & Seq
Reverse (-)
34388935 .. 34390900
1966 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19395

Sequence Viewer

Length: 990 bp
ATGGCGGGTGAGAACAAAAAAGTGTGCGTAACTGGAGCTGGAGGATTTGCTGCCTCTTGGGTCGTCAAGCTTCTTCTCTCTCAGGACTATCTTGTCCATGGAACCGTCAGACAACCAGGTGATAGCAAGTACGACCACTTGAACAGCCTTGAGAAAGCATCAGAGAACCTGAAACTGATCAAGGCAGATTTGCTGGACTATGATTCTCTATGTTCCGCAATTTCGGGATGCAGCGGTGTGTTCCATGTTGCCAGTCCTGTACCAACAACCGAAGTAACAAACCCCGAAGCAGAAGTAGTTGAACCAGCTGTGAAGGGAACGCTTAATGTGCTGAAAGCTTGCTTGGAGAACAAGGTGAAGAGAGTTGTTGTTGTGTCTTCTGTAGCTGCTGTTGTGATGAACCCGAACTGGCCCAAGGATAAAGTCAAGGATGAAAGCTGTTGGTCTGATCCTGAATATATTAAAACCACTAGTCTTGAATCCATGAAAACTTCTAAGAAATGGTATTATATTGCGAAAACACAAGCAGAGAGCGAGGCTCTGGAGTTTGGAAAAAGAACTGGACTTGAGGTTGTTACTGTTTGTCCTTCTGCCATTTTGGGTCCACTTTTGCAATCTACCCTAAATTCCAGTACCTTGCTCCTGCCTATGGGTCTCAAAGGTGGGTTCGGTTCGTTTGGATACAATTATTGGACTGTGGTGGATGTTCGTGACTTAGCTGAAGCACTGCTTATGGTTTACAAGAAATCTGACGCTGGAGAAAGATATATGTGCACATCACATGCAATTGGGGTGGAGGAAGTGGTGGAGAAATACTTGAAGATTTCATATCCCAACTACAATTATTCCGACAAGCTTAGTCACACAGAGGAAGAAGAAGAGAAACTGAGTTCGGAAAAGTTGCAGAAGTTGGGTTGGAGTTACCGACCATTGGAGGAAACTCTAATTGATTCCATTGAATGCTGCCGGAAGATGGGGGTCTTGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

329

Amino Acids

36.29

Weight (kDa)

5.47

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 138 3.2e-08 NmrA-like family
Epimerase PF01370 8 - 255 1e-22 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 8 - 131 2e-08 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 10 - 147 2.2e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 127 8.6e-13 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 206 1.2e-09 Male sterility protein
KR PF08659 10 - 135 2.9e-06 KR domain
NAD_binding_10 PF13460 12 - 132 1.1e-12 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AciI CCGC 3 cut(s) 5, 216, 234
AclWI GGATC 1 cut(s) 443
AcsI RAATTY 1 cut(s) 625
AcuI CTGAAG 1 cut(s) 741
AfaI GTAC 3 cut(s) 131, 261, 634
AfiI CCNNNNNNNGG 3 cut(s) 649, 931, 973
AgsI TTSAA 5 cut(s) 142, 302, 479, 820, 959
AhlI ACTAGT 1 cut(s) 470
AjnI CCWGG 1 cut(s) 115
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 8 cut(s) 38, 70, 308, 338, 386, 438, 719, 856
AluI AGCT 8 cut(s) 38, 70, 308, 338, 386, 438, 719, 856
Alw21I GWGCWC 1 cut(s) 776
Alw26I GTCTC 1 cut(s) 659
Alw44I GTGCAC 1 cut(s) 772
AlwI GGATC 1 cut(s) 443
AoxI GGCC 1 cut(s) 410
ApaLI GTGCAC 1 cut(s) 772
ApeKI GCWGC 4 cut(s) 50, 231, 386, 963
ApoI RAATTY 1 cut(s) 625
AspS9I GGNCC 2 cut(s) 411, 602
AsuHPI GGTGA 3 cut(s) 20, 131, 367
AvaII GGWCC 1 cut(s) 602
BaeGI GKGCMC 1 cut(s) 776
BbsI GAAGAC 1 cut(s) 369
Bbv12I GWGCWC 1 cut(s) 776
BbvI GCAGC 4 cut(s) 37, 243, 373, 950
BccI CCATC 1 cut(s) 967
BciT130I CCWGG 1 cut(s) 117
BciVI GTATCC 1 cut(s) 674
BclI TGATCA 1 cut(s) 177
BcoDI GTCTC 1 cut(s) 659
BcuI ACTAGT 1 cut(s) 470
BfaI CTAG 1 cut(s) 471
BfmI CTRYAG 1 cut(s) 381
BfuI GTATCC 1 cut(s) 674
BisI GCNGC 4 cut(s) 51, 232, 387, 964
BlsI GCNGC 4 cut(s) 52, 233, 388, 965
Bme1390I CCNGG 1 cut(s) 117
Bme18I GGWCC 1 cut(s) 602
BmgT120I GGNCC 2 cut(s) 411, 602
BmiI GGNNCC 2 cut(s) 103, 603
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 2 cut(s) 167, 218
BpiI GAAGAC 1 cut(s) 369
BplI GAGNNNNNCTC 2 cut(s) 523, 555
BpmI CTGGAG 4 cut(s) 54, 60, 563, 777
BpuEI CTTGAG 2 cut(s) 170, 587
BsaI GGTCTC 1 cut(s) 659
BsaJI CCNNGG 2 cut(s) 97, 414
Bsc4I CCNNNNNNNGG 3 cut(s) 649, 931, 973
Bse1I ACTGG 5 cut(s) 37, 252, 413, 565, 630
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 2 cut(s) 97, 414
BseGI GGATG 3 cut(s) 233, 436, 709
BseLI CCNNNNNNNGG 3 cut(s) 649, 931, 973
BseMII CTCAG 2 cut(s) 95, 878
BseNI ACTGG 5 cut(s) 37, 252, 413, 565, 630
BseSI GKGCMC 1 cut(s) 776
BseXI GCAGC 4 cut(s) 37, 243, 373, 950
BshFI GGCC 1 cut(s) 412
BsiHKAI GWGCWC 1 cut(s) 776
BsiSI CCGG 1 cut(s) 967
BslI CCNNNNNNNGG 3 cut(s) 649, 931, 973
BsmAI GTCTC 1 cut(s) 659
BsmI GAATGC 1 cut(s) 965
BsnI GGCC 1 cut(s) 412
Bso31I GGTCTC 1 cut(s) 659
Bsp1286I GDGCHC 1 cut(s) 776
Bsp143I GATC 2 cut(s) 177, 448
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 3 cut(s) 5, 216, 234
BspANI GGCC 1 cut(s) 412
BspCNI CTCAG 2 cut(s) 94, 879
BspLI GGNNCC 2 cut(s) 103, 603
BspPI GGATC 1 cut(s) 443
BspTNI GGTCTC 1 cut(s) 659
BsrI ACTGG 5 cut(s) 37, 252, 413, 565, 630
BssECI CCNNGG 2 cut(s) 97, 414
BssMI GATC 2 cut(s) 177, 448
BssT1I CCWWGG 2 cut(s) 97, 414
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 3 cut(s) 106, 580, 697
Bst6I CTCTTC 2 cut(s) 353, 873
BstC8I GCNNGC 1 cut(s) 340
BstDEI CTNAG 5 cut(s) 81, 495, 715, 857, 887
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 3 cut(s) 233, 436, 709
BstKTI GATC 2 cut(s) 180, 451
BstMAI GTCTC 1 cut(s) 659
BstMBI GATC 2 cut(s) 177, 448
BstMWI GCNNNNNNNGC 1 cut(s) 328
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 785
BstSCI CCNGG 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 381
BstSLI GKGCMC 1 cut(s) 776
BstV1I GCAGC 4 cut(s) 37, 243, 373, 950
BstV2I GAAGAC 1 cut(s) 369
BsuI GTATCC 1 cut(s) 674
BsuRI GGCC 1 cut(s) 412
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 3 cut(s) 233, 436, 709
BtsI GCAGTG 1 cut(s) 725
BtsIMutI CAGTG 1 cut(s) 725
Cac8I GCNNGC 1 cut(s) 340
Cfr13I GGNCC 2 cut(s) 411, 602
CseI GACGC 1 cut(s) 761
CsiI ACCWGGT 1 cut(s) 115
Csp6I GTAC 3 cut(s) 130, 260, 633
CspCI CAANNNNNGTGG 4 cut(s) 457, 492, 774, 809
CviAII CATG 4 cut(s) 98, 245, 484, 782
CviQI GTAC 3 cut(s) 130, 260, 633
DdeI CTNAG 5 cut(s) 81, 495, 715, 857, 887
DpnI GATC 2 cut(s) 179, 450
DpnII GATC 2 cut(s) 177, 448
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
Eam1104I CTCTTC 2 cut(s) 353, 873
EarI CTCTTC 2 cut(s) 353, 873
Eco130I CCWWGG 2 cut(s) 97, 414
Eco31I GGTCTC 1 cut(s) 659
Eco47I GGWCC 1 cut(s) 602
Eco57I CTGAAG 1 cut(s) 741
EcoRII CCWGG 1 cut(s) 115
EcoT14I CCWWGG 2 cut(s) 97, 414
ErhI CCWWGG 2 cut(s) 97, 414
FaeI CATG 4 cut(s) 101, 248, 487, 785
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FatI CATG 4 cut(s) 97, 244, 483, 781
FbaI TGATCA 1 cut(s) 177
Fnu4HI GCNGC 4 cut(s) 51, 232, 387, 964
FokI GGATG 3 cut(s) 240, 443, 716
Fsp4HI GCNGC 4 cut(s) 51, 232, 387, 964
FspBI CTAG 1 cut(s) 471
GluI GCNGC 4 cut(s) 51, 232, 387, 964
GsuI CTGGAG 4 cut(s) 54, 60, 563, 777
HaeIII GGCC 1 cut(s) 412
HapII CCGG 1 cut(s) 967
HgaI GACGC 1 cut(s) 761
Hin1II CATG 4 cut(s) 101, 248, 487, 785
HindIII AAGCTT 3 cut(s) 68, 336, 854
HinfI GANTC 3 cut(s) 203, 479, 950
HpaII CCGG 1 cut(s) 967
HphI GGTGA 3 cut(s) 20, 131, 367
Hpy166II GTNNAC 3 cut(s) 605, 739, 774
Hpy188I TCNGA 6 cut(s) 110, 163, 448, 751, 850, 895
Hpy188III TCNNGA 6 cut(s) 83, 225, 452, 476, 542, 710
Hpy8I GTNNAC 3 cut(s) 605, 739, 774
HpyAV CCTTC 2 cut(s) 307, 597
HpyCH4III ACNGT 3 cut(s) 106, 580, 697
HpyCH4V TGCA 5 cut(s) 231, 613, 774, 785, 904
HpyF10VI GCNNNNNNNGC 1 cut(s) 328
HpyF3I CTNAG 5 cut(s) 81, 495, 715, 857, 887
Hsp92II CATG 4 cut(s) 101, 248, 487, 785
Ksp22I TGATCA 1 cut(s) 177
Kzo9I GATC 2 cut(s) 177, 448
LmnI GCTCC 2 cut(s) 35, 645
Lsp1109I GCAGC 4 cut(s) 37, 243, 373, 950
LweI GCATC 2 cut(s) 167, 218
MabI ACCWGGT 1 cut(s) 115
MaeI CTAG 1 cut(s) 471
MaeIII GTNAC 6 cut(s) 28, 274, 574, 710, 860, 920
MalI GATC 2 cut(s) 179, 450
MboI GATC 2 cut(s) 177, 448
MboII GAAGA 8 cut(s) 65, 369, 370, 832, 884, 887, 890, 982
MfeI CAATTG 1 cut(s) 786
MhlI GDGCHC 1 cut(s) 776
MluCI AATT 6 cut(s) 219, 625, 685, 786, 841, 945
MmeI TCCRAC 2 cut(s) 873, 896
MnlI CCTC 7 cut(s) 35, 64, 529, 562, 790, 862, 928
MseI TTAA 2 cut(s) 324, 462
MspA1I CMGCKG 2 cut(s) 234, 308
MspI CCGG 1 cut(s) 967
MspR9I CCNGG 1 cut(s) 117
MunI CAATTG 1 cut(s) 786
Mva1269I GAATGC 1 cut(s) 965
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 1 cut(s) 328
NcoI CCATGG 1 cut(s) 97
NdeII GATC 2 cut(s) 177, 448
NlaIII CATG 4 cut(s) 101, 248, 487, 785
NlaIV GGNNCC 2 cut(s) 103, 603
NmuCI GTSAC 2 cut(s) 710, 860
NspI RCATGY 1 cut(s) 785
PctI GAATGC 1 cut(s) 965
PfeI GAWTC 3 cut(s) 203, 479, 950
PkrI GCNGC 4 cut(s) 52, 233, 388, 965
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 2 cut(s) 103, 603
PspPI GGNCC 2 cut(s) 411, 602
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 3 cut(s) 131, 261, 634
RsaNI GTAC 3 cut(s) 130, 260, 633
SaqAI TTAA 2 cut(s) 324, 462
SatI GCNGC 4 cut(s) 51, 232, 387, 964
Sau3AI GATC 2 cut(s) 177, 448
Sau96I GGNCC 2 cut(s) 411, 602
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 1 cut(s) 776
SexAI ACCWGGT 1 cut(s) 115
SfaNI GCATC 2 cut(s) 167, 218
SfcI CTRYAG 1 cut(s) 381
SinI GGWCC 1 cut(s) 602
SmlI CTYRAG 2 cut(s) 149, 566
SmoI CTYRAG 2 cut(s) 149, 566
SpeI ACTAGT 1 cut(s) 470
Sse9I AATT 6 cut(s) 219, 625, 685, 786, 841, 945
SsiI CCGC 3 cut(s) 5, 216, 234
SspMI CTAG 1 cut(s) 471
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 2 cut(s) 97, 414
TaaI ACNGT 3 cut(s) 106, 580, 697
TasI AATT 6 cut(s) 219, 625, 685, 786, 841, 945
TfiI GAWTC 3 cut(s) 203, 479, 950
Tru1I TTAA 2 cut(s) 324, 462
Tru9I TTAA 2 cut(s) 324, 462
TscAI CASTG 1 cut(s) 732
TseFI GTSAC 2 cut(s) 710, 860
TseI GCWGC 4 cut(s) 50, 231, 386, 963
Tsp45I GTSAC 2 cut(s) 710, 860
TspDTI ATGAA 4 cut(s) 413, 447, 500, 816
TspRI CASTG 1 cut(s) 732
VneI GTGCAC 1 cut(s) 772
VpaK11BI GGWCC 1 cut(s) 602
XapI RAATTY 1 cut(s) 625
XceI RCATGY 1 cut(s) 785
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.