Rh7BG294600

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001551
Physical Location & Seq
Reverse (-)
27696984 .. 27698601
1618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG294600.1

Sequence Viewer

Length: 969 bp
ATGGCGGATGCGTATGAAACAGTGTGTGTAACTGGAGCCGGAGGGTTTGCTGCCTCCTGGATTGTCAAGCTTCTACTCTCCAACAACTATGTCATCCATGGAACCGTCAGACAACCAGGGGATAGCAAGTATGATCACTTGAACAAGCTGGAGAGATCACCGGAGAACCTGAAGCTGTTCAAGGCAGATTTGCTGGATTATGAGTCTCTGAGATTGGCGATTTCAGGATGCAGTGGAGTGTTCCACGTGGCCAGTCCTGTACCTTTTCCAACTGCCATAACAGACCCCGAAGCAGAAGTACTTAAACCAGCTGTGGAGGGAACCCTTAATGTATTGAAAGCTTGCGTAGAGGCTAAAGTGAAGAGAGTTGTTGTTGTGTCGTCTATAGCTGCTGTTGTGAAGAACCCGAATTGGCCCAAGGATCAAGTGAAAGACGAGAGATGTTGGTCTGATCCGGAATATGTTAGGTCCACTAAGAATTGGTATTATCTTTCCAAAACACAAGCAGAAGCTGCAGCCTTGGAGTTCGGAAGAAGCAGTGGACTTGATGTTGTAACTGTTTGTCCTTCTCTCATCATGGGGCCACTGTTGCAATCCACTCTGAATACTAGTGGCTGGCTCCTGCCTATGGGATTCAAAGGCGGGTTTGAGAAGGTGGGCTACAATTACTGGACCGTGGTGGATGCTCGTGATTTAGCTGATGCACTGCTTCTTGTATACAAGAAATCTGAGGCTGGAGAAAGATACATATGCACAGCACATGCAATTGGGGTAGAAGAAGTAGTGGAGAAGTACTTGAAGATTTCACATCCCAACTACAACTATTCTCCCGAGCTTACGCATACGAAGGAAGAAGAGGAGAAGCTGAGTTCGGAAAAATTGGAGAGGTTGGGTTGGAGTTACCAGCCATTGGGGGAAACTCTTATTGATTCCATTGAAAGCTACCAGAAGATTGGAGTCATGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

322

Amino Acids

35.71

Weight (kDa)

5.42

Isoelectric Point (pI)

35.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 140 1e-07 NmrA-like family
Epimerase PF01370 8 - 248 9.7e-24 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 8 - 132 2.5e-07 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 10 - 199 1e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 266 9.1e-14 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 203 1.2e-10 Male sterility protein
KR PF08659 10 - 136 6.2e-06 KR domain
NAD_binding_10 PF13460 12 - 190 7.8e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 910
AccI GTMKAC 1 cut(s) 717
AccIII TCCGGA 1 cut(s) 454
AciI CCGC 2 cut(s) 5, 642
AclWI GGATC 2 cut(s) 429, 446
AcoI YGGCCR 1 cut(s) 249
AcuI CTGAAG 1 cut(s) 191
AcvI CACGTG 1 cut(s) 247
AfaI GTAC 3 cut(s) 261, 300, 794
AfiI CCNNNNNNNGG 2 cut(s) 628, 910
AgsI TTSAA 6 cut(s) 142, 181, 337, 637, 799, 938
AhlI ACTAGT 1 cut(s) 608
AjnI CCWGG 2 cut(s) 56, 115
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 2 cut(s) 429, 446
AlwNI CAGNNNCTG 1 cut(s) 512
Ama87I CYCGRG 1 cut(s) 830
Aor13HI TCCGGA 1 cut(s) 454
AoxI GGCC 3 cut(s) 249, 413, 581
ApeKI GCWGC 4 cut(s) 50, 389, 512, 515
Asp700I GAANNNNTTC 1 cut(s) 176
AspS9I GGNCC 4 cut(s) 414, 468, 581, 672
AsuHPI GGTGA 1 cut(s) 150
AvaI CYCGRG 1 cut(s) 830
AvaII GGWCC 2 cut(s) 468, 672
BalI TGGCCA 1 cut(s) 251
BarI GAAGNNNNNNTAC 4 cut(s) 282, 314, 644, 676
BauI CACGAG 1 cut(s) 687
BbrPI CACGTG 1 cut(s) 247
BbvI GCAGC 4 cut(s) 37, 376, 499, 527
BciT130I CCWGG 2 cut(s) 58, 117
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 210
BcuI ACTAGT 1 cut(s) 608
BfaI CTAG 1 cut(s) 609
BfmI CTRYAG 2 cut(s) 384, 513
BisI GCNGC 4 cut(s) 51, 390, 513, 516
BlsI GCNGC 4 cut(s) 52, 391, 514, 517
BmcAI AGTACT 2 cut(s) 300, 794
Bme1390I CCNGG 2 cut(s) 58, 117
Bme18I GGWCC 2 cut(s) 468, 672
BmeT110I CYCGRG 1 cut(s) 830
BmgT120I GGNCC 4 cut(s) 414, 468, 581, 672
BmiI GGNNCC 5 cut(s) 37, 103, 322, 582, 620
BmrFI CCNGG 2 cut(s) 58, 117
BmsI GCATC 3 cut(s) 218, 673, 691
BpmI CTGGAG 3 cut(s) 54, 170, 756
BsaAI YACGTR 1 cut(s) 247
BsaJI CCNNGG 5 cut(s) 97, 116, 417, 519, 675
BsaWI WCCGGW 2 cut(s) 160, 454
Bsc4I CCNNNNNNNGG 2 cut(s) 628, 910
Bse1I ACTGG 3 cut(s) 37, 252, 674
BseAI TCCGGA 1 cut(s) 454
BseBI CCWGG 2 cut(s) 58, 117
BseDI CCNNGG 5 cut(s) 97, 116, 417, 519, 675
BseGI GGATG 5 cut(s) 13, 93, 233, 688, 808
BseLI CCNNNNNNNGG 2 cut(s) 628, 910
BseMII CTCAG 3 cut(s) 200, 720, 857
BseNI ACTGG 3 cut(s) 37, 252, 674
BseRI GAGGAG 1 cut(s) 872
BseXI GCAGC 4 cut(s) 37, 376, 499, 527
BshFI GGCC 3 cut(s) 251, 415, 583
BsiHKCI CYCGRG 1 cut(s) 830
BsiSI CCGG 3 cut(s) 39, 161, 455
BslI CCNNNNNNNGG 2 cut(s) 628, 910
BsmAI GTCTC 1 cut(s) 210
BsnI GGCC 3 cut(s) 251, 415, 583
BsoBI CYCGRG 1 cut(s) 830
Bsp13I TCCGGA 1 cut(s) 454
Bsp143I GATC 4 cut(s) 133, 155, 421, 451
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 2 cut(s) 5, 642
BspANI GGCC 3 cut(s) 251, 415, 583
BspCNI CTCAG 3 cut(s) 201, 721, 858
BspEI TCCGGA 1 cut(s) 454
BspLI GGNNCC 5 cut(s) 37, 103, 322, 582, 620
BspMAI CTGCAG 1 cut(s) 517
BspPI GGATC 2 cut(s) 429, 446
BsrI ACTGG 3 cut(s) 37, 252, 674
BssECI CCNNGG 5 cut(s) 97, 116, 417, 519, 675
BssMI GATC 4 cut(s) 133, 155, 421, 451
BssNAI GTATAC 1 cut(s) 718
BssSI CACGAG 1 cut(s) 687
BssT1I CCWWGG 3 cut(s) 97, 417, 519
Bst1107I GTATAC 1 cut(s) 718
Bst2BI CACGAG 1 cut(s) 687
Bst2UI CCWGG 2 cut(s) 58, 117
Bst4CI ACNGT 5 cut(s) 22, 106, 559, 588, 676
Bst6I CTCTTC 2 cut(s) 356, 849
BstAPI GCANNNNNTGC 1 cut(s) 512
BstBAI YACGTR 1 cut(s) 247
BstC8I GCNNGC 2 cut(s) 343, 617
BstDEI CTNAG 4 cut(s) 209, 474, 729, 866
BstDSI CCRYGG 2 cut(s) 97, 675
BstF5I GGATG 5 cut(s) 13, 93, 233, 688, 808
BstKTI GATC 4 cut(s) 136, 158, 424, 454
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 4 cut(s) 133, 155, 421, 451
BstMWI GCNNNNNNNGC 2 cut(s) 512, 589
BstNI CCWGG 2 cut(s) 58, 117
BstNSI RCATGY 1 cut(s) 764
BstSCI CCNGG 2 cut(s) 56, 115
BstSFI CTRYAG 2 cut(s) 384, 513
BstV1I GCAGC 4 cut(s) 37, 376, 499, 527
BstXI CCANNNNNNTGG 1 cut(s) 953
BstZ17I GTATAC 1 cut(s) 718
BsuRI GGCC 3 cut(s) 251, 415, 583
BtgI CCRYGG 2 cut(s) 97, 675
BtsCI GGATG 5 cut(s) 13, 93, 233, 688, 808
BtsI GCAGTG 3 cut(s) 238, 544, 704
BtsIMutI CAGTG 5 cut(s) 27, 238, 544, 584, 704
Cac8I GCNNGC 2 cut(s) 343, 617
CaiI CAGNNNCTG 1 cut(s) 512
Cfr13I GGNCC 4 cut(s) 414, 468, 581, 672
Csp6I GTAC 3 cut(s) 260, 299, 793
CviAII CATG 4 cut(s) 98, 577, 761, 961
CviQI GTAC 3 cut(s) 260, 299, 793
DdeI CTNAG 4 cut(s) 209, 474, 729, 866
DpnI GATC 4 cut(s) 135, 157, 423, 453
DpnII GATC 4 cut(s) 133, 155, 421, 451
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 2 cut(s) 356, 849
EarI CTCTTC 2 cut(s) 356, 849
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 97, 417, 519
Eco47I GGWCC 2 cut(s) 468, 672
Eco57I CTGAAG 1 cut(s) 191
Eco72I CACGTG 1 cut(s) 247
Eco88I CYCGRG 1 cut(s) 830
EcoRII CCWGG 2 cut(s) 56, 115
EcoT14I CCWWGG 3 cut(s) 97, 417, 519
ErhI CCWWGG 3 cut(s) 97, 417, 519
FaeI CATG 4 cut(s) 101, 580, 764, 964
FatI CATG 4 cut(s) 97, 576, 760, 960
FauI CCCGC 1 cut(s) 635
FauNDI CATATG 1 cut(s) 749
FbaI TGATCA 1 cut(s) 133
FblI GTMKAC 1 cut(s) 717
Fnu4HI GCNGC 4 cut(s) 51, 390, 513, 516
FokI GGATG 5 cut(s) 20, 80, 240, 695, 795
Fsp4HI GCNGC 4 cut(s) 51, 390, 513, 516
FspBI CTAG 1 cut(s) 609
GluI GCNGC 4 cut(s) 51, 390, 513, 516
GsuI CTGGAG 3 cut(s) 54, 170, 756
HaeIII GGCC 3 cut(s) 251, 415, 583
HapII CCGG 3 cut(s) 39, 161, 455
Hin1II CATG 4 cut(s) 101, 580, 764, 964
HindIII AAGCTT 2 cut(s) 68, 339
HinfI GANTC 4 cut(s) 203, 633, 929, 957
HpaII CCGG 3 cut(s) 39, 161, 455
HphI GGTGA 1 cut(s) 150
Hpy166II GTNNAC 3 cut(s) 471, 542, 718
Hpy188I TCNGA 7 cut(s) 110, 210, 451, 530, 603, 730, 874
Hpy188III TCNNGA 4 cut(s) 225, 455, 689, 830
Hpy8I GTNNAC 3 cut(s) 471, 542, 718
HpyAV CCTTC 3 cut(s) 576, 646, 841
HpyCH4III ACNGT 5 cut(s) 22, 106, 559, 588, 676
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 6 cut(s) 231, 515, 592, 704, 753, 764
HpyF10VI GCNNNNNNNGC 2 cut(s) 512, 589
HpyF3I CTNAG 4 cut(s) 209, 474, 729, 866
HpySE526I ACGT 1 cut(s) 246
Hsp92II CATG 4 cut(s) 101, 580, 764, 964
Kpn2I TCCGGA 1 cut(s) 454
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 4 cut(s) 133, 155, 421, 451
LmnI GCTCC 2 cut(s) 35, 624
Lsp1109I GCAGC 4 cut(s) 37, 376, 499, 527
LweI GCATC 3 cut(s) 218, 673, 691
MaeI CTAG 1 cut(s) 609
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 3 cut(s) 28, 553, 899
MalI GATC 4 cut(s) 135, 157, 423, 453
MboI GATC 4 cut(s) 133, 155, 421, 451
MboII GAAGA 8 cut(s) 373, 412, 543, 788, 811, 863, 866, 961
MfeI CAATTG 1 cut(s) 765
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 5 cut(s) 409, 478, 664, 765, 878
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 2 cut(s) 212, 966
MmeI TCCRAC 3 cut(s) 105, 293, 875
MnlI CCTC 7 cut(s) 35, 64, 310, 343, 724, 850, 879
Mox20I TGGCCA 1 cut(s) 251
MroI TCCGGA 1 cut(s) 454
MroXI GAANNNNTTC 1 cut(s) 176
MscI TGGCCA 1 cut(s) 251
MseI TTAA 2 cut(s) 303, 327
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 311
MspI CCGG 3 cut(s) 39, 161, 455
MspR9I CCNGG 2 cut(s) 58, 117
MunI CAATTG 1 cut(s) 765
MvaI CCWGG 2 cut(s) 58, 117
MwoI GCNNNNNNNGC 2 cut(s) 512, 589
NcoI CCATGG 1 cut(s) 97
NdeI CATATG 1 cut(s) 749
NdeII GATC 4 cut(s) 133, 155, 421, 451
NlaIII CATG 4 cut(s) 101, 580, 764, 964
NlaIV GGNNCC 5 cut(s) 37, 103, 322, 582, 620
NspI RCATGY 1 cut(s) 764
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 2 cut(s) 633, 929
PflMI CCANNNNNTGG 1 cut(s) 910
PfoI TCCNGGA 1 cut(s) 56
PkrI GCNGC 4 cut(s) 52, 391, 514, 517
PleI GAGTC 2 cut(s) 211, 965
PmaCI CACGTG 1 cut(s) 247
PmlI CACGTG 1 cut(s) 247
PpsI GAGTC 2 cut(s) 211, 965
Ppu21I YACGTR 1 cut(s) 247
Psp6I CCWGG 2 cut(s) 56, 115
PspCI CACGTG 1 cut(s) 247
PspGI CCWGG 2 cut(s) 56, 115
PspN4I GGNNCC 5 cut(s) 37, 103, 322, 582, 620
PspPI GGNCC 4 cut(s) 414, 468, 581, 672
PstI CTGCAG 1 cut(s) 517
PstNI CAGNNNCTG 1 cut(s) 512
PvuII CAGCTG 1 cut(s) 311
RsaI GTAC 3 cut(s) 261, 300, 794
RsaNI GTAC 3 cut(s) 260, 299, 793
SaqAI TTAA 2 cut(s) 303, 327
SatI GCNGC 4 cut(s) 51, 390, 513, 516
Sau3AI GATC 4 cut(s) 133, 155, 421, 451
Sau96I GGNCC 4 cut(s) 414, 468, 581, 672
ScaI AGTACT 2 cut(s) 300, 794
SchI GAGTC 2 cut(s) 212, 966
ScrFI CCNGG 2 cut(s) 58, 117
SfaNI GCATC 3 cut(s) 218, 673, 691
SfcI CTRYAG 2 cut(s) 384, 513
SinI GGWCC 2 cut(s) 468, 672
SpeI ACTAGT 1 cut(s) 608
Sse9I AATT 5 cut(s) 409, 478, 664, 765, 878
SsiI CCGC 2 cut(s) 5, 642
SspMI CTAG 1 cut(s) 609
StyD4I CCNGG 2 cut(s) 56, 115
StyI CCWWGG 3 cut(s) 97, 417, 519
TaaI ACNGT 5 cut(s) 22, 106, 559, 588, 676
TaiI ACGT 1 cut(s) 249
TasI AATT 5 cut(s) 409, 478, 664, 765, 878
TatI WGTACW 2 cut(s) 298, 792
TfiI GAWTC 2 cut(s) 633, 929
Tru1I TTAA 2 cut(s) 303, 327
Tru9I TTAA 2 cut(s) 303, 327
TscAI CASTG 5 cut(s) 27, 238, 544, 591, 711
TseI GCWGC 4 cut(s) 50, 389, 512, 515
TspDTI ATGAA 1 cut(s) 30
TspRI CASTG 5 cut(s) 27, 238, 544, 591, 711
Van91I CCANNNNNTGG 1 cut(s) 910
VpaK11BI GGWCC 2 cut(s) 468, 672
XceI RCATGY 1 cut(s) 764
XmiI GTMKAC 1 cut(s) 717
XmnI GAANNNNTTC 1 cut(s) 176
XspI CTAG 1 cut(s) 609
ZrmI AGTACT 2 cut(s) 300, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.