Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001551

Physical Location & Seq

Genomic Coordinates Forward (+)

387544 .. 390928

Gene Structure 3385 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1003300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 948 bp

ATGGCGGGTGAGGCGGCTGATCAGAAAGAAAAAGTATGCGTGACTGGAGCTGGAGGATTTACTGCCTCTTGGGTCGTCAACCTCCTTCTCTCCAGGGACTATGTTGTTCATGGAACTGTCAGGCAACCAGGAGATAGCAAGTATGCTCACTTGAACAAGCTTGAGAAAGCATCAGTGAACCTCAAACTGTTCAAGGCAGATTTGTTGGACTACGACTCTCTTCGTTTGGCCATTGAAGGTTGCAGCGGAGTCTTCCATGTTGCCAGCCCTGAGTTTCTTGAACCAGCAATAAAGGGAACGCTGAATGTACTCAAGGCTTGCAAAAAGGCCAAAGTGAAGAGAGTTGTAGTTGTGTCGTCTATAGCCGCCGTCGTTATGAACCCGGAGTGGCCTAAGGATCAAGTCAAGGACGAGACTTGTTGGTCCGTTCCCGAATATATAAAAACAACAAAGAAATGGTACTATCTTTCGAAAACAGAAGCAGAACGCGAGGCTCTGGAGTTTGGAAAAAGAAATGGACTTGAGGTTGTGACTGTTTGTCCTTCTATCATTTTGGGGCCAATCTTGCAATCCACTCTCAATTCCAGTAGCTCTCTCATTGTTGCAACTATAATAGGTGGTCTTGAGTCCTTGGGATGCAATTACTGGACGTTCGTGGATGTTCGTGATTTAGCTGACGCGCTGCTTCTTGCATACAACAAGCCTGAGGCTGCAGGAGAAAGATACTTATGCAATTCACACTCAATTGGCATACGAGATGTGGTGGAGAAATACTTAAGGCCTACATATCCCGATTACAAGTACCCCAAGAACCTTACCTATGCAGAGGAAGAATTTCAACACTTTAGTTCCGAAAAATTGCAGAAGTTGGGTTGGACTTTCCGGCCAGTTGAGGAAACGCTTAATGATTCTATTGAAAGCTACCGGAAGGCTGGAATCGTGGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Epimerase

Protein Analysis

316

Amino Acids

35.07

Weight (kDa)

6.96

Isoelectric Point (pI)

37.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NmrA	PF05368	8 - 136	1.3e-11	NmrA-like family
Epimerase	PF01370	12 - 241	1.4e-22	NAD dependent epimerase/dehydratase family
3Beta_HSD	PF01073	14 - 200	6.5e-15	3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd	PF16363	14 - 120	1.5e-09	GDP-mannose 4,6 dehydratase
NAD_binding_10	PF13460	16 - 181	1.2e-15	NAD(P)H-binding
NAD_binding_4	PF07993	91 - 192	7.4e-08	Male sterility protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001551)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76470
fragaria_vesca	FvH4_5g26030 FvH4_5g26030 FvH4_5g26090
malus_domestica	MD06G1003300.v1.1 MD06G1003700.v1.1 MD06G1003800.v1.1 MD06G1003900.v1.1
prunus_persica	Prupe.5G004900_v2.0.a1 Prupe.5G005000_v2.0.a1
pyrus_communis	pycom06g00340 pycom06g00350 pycom06g00360 pycom06g00380
rosa_chinensis	RchiOBHm_Chr7g0216711 RchiOBHm_Chr7g0216751 RchiOBHm_Chr7g0216801
rosa_laevigata	RLG00000002541 RLG00000002544
rosa_multiflora	Rmu_co8327343.1_g000001 Rmu_sc0000125.1_g000013 Rmu_sc0003814.1_g000008 Rmu_sc0003814.1_g000009 Rmu_sc0004941.1_g000004 Rmu_ssc0000117.1_g000016 Rmu_ssc0000117.1_g000017 Rmu_ssc0000375.1_g000001
rosa_roxburghii	Rroxscaffold_3G00242850 Rroxscaffold_3G00242960 Rroxscaffold_3G00242980 Rroxscaffold_5G00345210 Rroxscaffold_7G00166270
rosa_rugosa	Rorug07G0161900 Rorug07G0162100
rosa_samantha	Rh7AG301800 Rh7AG302000 Rh7AG302900 Rh7BG293600 Rh7BG293800 Rh7BG294600 Rh7CG320500 Rh7CG320800 Rh7CG321500 Rh7DG302500 Rh7DG302900 Rh7DG303100 Rh7DG303200
rosa_wichuraiana	Rw0G009080 Rw7G025620 Rw7G025730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	421
AccII	CGCG	2 cut(s)	489, 680
AciI	CCGC	4 cut(s)	5, 14, 246, 366
AclWI	GGATC	1 cut(s)	405
AcoI	YGGCCR	2 cut(s)	228, 884
AcsI	RAATTY	1 cut(s)	833
AfaI	GTAC	3 cut(s)	309, 461, 803
AflII	CTTAAG	1 cut(s)	775
AgsI	TTSAA	6 cut(s)	154, 193, 236, 281, 839, 917
AhdI	GACNNNNNGTC	1 cut(s)	537
AjnI	CCWGG	2 cut(s)	92, 127
AluBI	AGCT	5 cut(s)	50, 160, 591, 674, 921
AluI	AGCT	5 cut(s)	50, 160, 591, 674, 921
Alw26I	GTCTC	1 cut(s)	407
AlwI	GGATC	1 cut(s)	405
AoxI	GGCC	6 cut(s)	228, 327, 389, 557, 779, 884
ApeKI	GCWGC	3 cut(s)	243, 682, 710
ApoI	RAATTY	1 cut(s)	833
Asp700I	GAANNNNTTC	1 cut(s)	834
AspLEI	GCGC	1 cut(s)	682
AspS9I	GGNCC	2 cut(s)	423, 557
AsuC2I	CCSGG	1 cut(s)	383
AsuHPI	GGTGA	1 cut(s)	20
AsuII	TTCGAA	1 cut(s)	470
AvaII	GGWCC	1 cut(s)	423
AxyI	CCTNAGG	2 cut(s)	393, 705
BalI	TGGCCA	1 cut(s)	230
BbsI	GAAGAC	1 cut(s)	244
BbvI	GCAGC	3 cut(s)	255, 669, 697
BceAI	ACGGC	1 cut(s)	353
BciT130I	CCWGG	2 cut(s)	94, 129
BclI	TGATCA	1 cut(s)	19
BcnI	CCSGG	1 cut(s)	383
BcoDI	GTCTC	1 cut(s)	407
BfmI	CTRYAG	2 cut(s)	360, 711
BfrI	CTTAAG	1 cut(s)	775
BisI	GCNGC	5 cut(s)	15, 244, 366, 683, 711
BlsI	GCNGC	5 cut(s)	16, 245, 367, 684, 712
Bme1390I	CCNGG	3 cut(s)	94, 129, 383
Bme18I	GGWCC	1 cut(s)	423
BmeRI	GACNNNNNGTC	1 cut(s)	537
BmgT120I	GGNCC	2 cut(s)	423, 557
BmiI	GGNNCC	1 cut(s)	558
BmrFI	CCNGG	3 cut(s)	94, 129, 383
BmsI	GCATC	2 cut(s)	179, 626
BpiI	GAAGAC	1 cut(s)	244
BpmI	CTGGAG	4 cut(s)	66, 72, 76, 518
Bpu14I	TTCGAA	1 cut(s)	470
BpuEI	CTTGAG	4 cut(s)	182, 296, 542, 644
BpuMI	CCSGG	1 cut(s)	383
BsaJI	CCNNGG	2 cut(s)	93, 630
BsaWI	WCCGGW	1 cut(s)	924
Bse1I	ACTGG	4 cut(s)	49, 585, 650, 887
Bse21I	CCTNAGG	2 cut(s)	393, 705
BseBI	CCWGG	2 cut(s)	94, 129
BseDI	CCNNGG	2 cut(s)	93, 630
BseGI	GGATG	2 cut(s)	641, 664
BseMII	CTCAG	2 cut(s)	261, 696
BseNI	ACTGG	4 cut(s)	49, 585, 650, 887
BseXI	GCAGC	3 cut(s)	255, 669, 697
Bsh1236I	CGCG	2 cut(s)	489, 680
BshFI	GGCC	6 cut(s)	230, 329, 391, 559, 781, 886
BsiSI	CCGG	3 cut(s)	383, 883, 925
BslFI	GGGAC	1 cut(s)	110
BsmAI	GTCTC	1 cut(s)	407
BsmFI	GGGAC	1 cut(s)	110
BsnI	GGCC	6 cut(s)	230, 329, 391, 559, 781, 886
Bsp119I	TTCGAA	1 cut(s)	470
Bsp143I	GATC	2 cut(s)	19, 397
BspACI	CCGC	4 cut(s)	5, 14, 246, 366
BspANI	GGCC	6 cut(s)	230, 329, 391, 559, 781, 886
BspCNI	CTCAG	2 cut(s)	262, 697
BspFNI	CGCG	2 cut(s)	489, 680
BspLI	GGNNCC	1 cut(s)	558
BspMAI	CTGCAG	1 cut(s)	715
BspPI	GGATC	1 cut(s)	405
BspT104I	TTCGAA	1 cut(s)	470
BspTI	CTTAAG	1 cut(s)	775
BsrI	ACTGG	4 cut(s)	49, 585, 650, 887
BssECI	CCNNGG	2 cut(s)	93, 630
BssMI	GATC	2 cut(s)	19, 397
BssT1I	CCWWGG	1 cut(s)	630
Bst2UI	CCWGG	2 cut(s)	94, 129
Bst4CI	ACNGT	3 cut(s)	118, 189, 535
Bst6I	CTCTTC	2 cut(s)	225, 332
BstAFI	CTTAAG	1 cut(s)	775
BstBI	TTCGAA	1 cut(s)	470
BstC8I	GCNNGC	2 cut(s)	265, 319
BstDEI	CTNAG	3 cut(s)	270, 393, 705
BstF5I	GGATG	2 cut(s)	641, 664
BstFNI	CGCG	2 cut(s)	489, 680
BstHHI	GCGC	1 cut(s)	682
BstKTI	GATC	2 cut(s)	22, 400
BstMAI	GTCTC	1 cut(s)	407
BstMBI	GATC	2 cut(s)	19, 397
BstMWI	GCNNNNNNNGC	2 cut(s)	11, 565
BstNI	CCWGG	2 cut(s)	94, 129
BstSCI	CCNGG	3 cut(s)	92, 127, 381
BstSFI	CTRYAG	2 cut(s)	360, 711
BstUI	CGCG	2 cut(s)	489, 680
BstV1I	GCAGC	3 cut(s)	255, 669, 697
BstV2I	GAAGAC	1 cut(s)	244
Bsu36I	CCTNAGG	2 cut(s)	393, 705
BsuRI	GGCC	6 cut(s)	230, 329, 391, 559, 781, 886
BtsCI	GGATG	2 cut(s)	641, 664
BtsIMutI	CAGTG	1 cut(s)	180
Cac8I	GCNNGC	2 cut(s)	265, 319
CfoI	GCGC	1 cut(s)	682
Cfr13I	GGNCC	2 cut(s)	423, 557
CseI	GACGC	1 cut(s)	686
Csp6I	GTAC	3 cut(s)	308, 460, 802
CviAII	CATG	2 cut(s)	110, 257
CviQI	GTAC	3 cut(s)	308, 460, 802
DdeI	CTNAG	3 cut(s)	270, 393, 705
DpnI	GATC	2 cut(s)	21, 399
DpnII	GATC	2 cut(s)	19, 397
DrdI	GACNNNNNNGTC	1 cut(s)	421
DriI	GACNNNNNGTC	1 cut(s)	537
DseDI	GACNNNNNNGTC	1 cut(s)	421
EaeI	YGGCCR	2 cut(s)	228, 884
Eam1104I	CTCTTC	2 cut(s)	225, 332
Eam1105I	GACNNNNNGTC	1 cut(s)	537
EarI	CTCTTC	2 cut(s)	225, 332
Eco130I	CCWWGG	1 cut(s)	630
Eco147I	AGGCCT	1 cut(s)	781
Eco47I	GGWCC	1 cut(s)	423
Eco81I	CCTNAGG	2 cut(s)	393, 705
EcoRII	CCWGG	2 cut(s)	92, 127
EcoT14I	CCWWGG	1 cut(s)	630
ErhI	CCWWGG	1 cut(s)	630
FaeI	CATG	2 cut(s)	113, 260
FaqI	GGGAC	1 cut(s)	110
FatI	CATG	2 cut(s)	109, 256
FbaI	TGATCA	1 cut(s)	19
Fnu4HI	GCNGC	5 cut(s)	15, 244, 366, 683, 711
FokI	GGATG	2 cut(s)	648, 671
Fsp4HI	GCNGC	5 cut(s)	15, 244, 366, 683, 711
GlaI	GCGC	1 cut(s)	681
GluI	GCNGC	5 cut(s)	15, 244, 366, 683, 711
GsuI	CTGGAG	4 cut(s)	66, 72, 76, 518
HaeIII	GGCC	6 cut(s)	230, 329, 391, 559, 781, 886
HapII	CCGG	3 cut(s)	383, 883, 925
HgaI	GACGC	1 cut(s)	686
HhaI	GCGC	1 cut(s)	682
Hin1II	CATG	2 cut(s)	113, 260
Hin6I	GCGC	1 cut(s)	680
HinP1I	GCGC	1 cut(s)	680
HincII	GTYRAC	1 cut(s)	79
HindII	GTYRAC	1 cut(s)	79
HindIII	AAGCTT	1 cut(s)	158
HinfI	GANTC	5 cut(s)	215, 249, 626, 908, 936
HpaII	CCGG	3 cut(s)	383, 883, 925
HphI	GGTGA	1 cut(s)	20
Hpy166II	GTNNAC	2 cut(s)	79, 178
Hpy188I	TCNGA	2 cut(s)	24, 853
Hpy188III	TCNNGA	6 cut(s)	278, 431, 497, 623, 665, 791
Hpy8I	GTNNAC	2 cut(s)	79, 178
Hpy99I	CGWCG	1 cut(s)	374
HpyAV	CCTTC	4 cut(s)	95, 230, 552, 922
HpyCH4III	ACNGT	3 cut(s)	118, 189, 535
HpyCH4IV	ACGT	1 cut(s)	650
HpyF10VI	GCNNNNNNNGC	2 cut(s)	11, 565
HpyF3I	CTNAG	3 cut(s)	270, 393, 705
HpySE526I	ACGT	1 cut(s)	650
Hsp92II	CATG	2 cut(s)	113, 260
HspAI	GCGC	1 cut(s)	680
Ksp22I	TGATCA	1 cut(s)	19
Kzo9I	GATC	2 cut(s)	19, 397
LmnI	GCTCC	1 cut(s)	47
Lsp1109I	GCAGC	3 cut(s)	255, 669, 697
LweI	GCATC	2 cut(s)	179, 626
MaeII	ACGT	1 cut(s)	650
MaeIII	GTNAC	2 cut(s)	40, 529
MalI	GATC	2 cut(s)	21, 399
MboI	GATC	2 cut(s)	19, 397
MboII	GAAGA	4 cut(s)	212, 244, 349, 842
MfeI	CAATTG	1 cut(s)	744
MlsI	TGGCCA	1 cut(s)	230
MluCI	AATT	6 cut(s)	580, 640, 733, 744, 833, 857
MluNI	TGGCCA	1 cut(s)	230
MlyI	GAGTC	3 cut(s)	209, 258, 635
MmeI	TCCRAC	2 cut(s)	186, 854
Mox20I	TGGCCA	1 cut(s)	230
MroXI	GAANNNNTTC	1 cut(s)	834
MscI	TGGCCA	1 cut(s)	230
MseI	TTAA	2 cut(s)	776, 903
Msp20I	TGGCCA	1 cut(s)	230
MspA1I	CMGCKG	1 cut(s)	246
MspCI	CTTAAG	1 cut(s)	775
MspI	CCGG	3 cut(s)	383, 883, 925
MspR9I	CCNGG	3 cut(s)	94, 129, 383
MunI	CAATTG	1 cut(s)	744
MvaI	CCWGG	2 cut(s)	94, 129
MvnI	CGCG	2 cut(s)	489, 680
MwoI	GCNNNNNNNGC	2 cut(s)	11, 565
NciI	CCSGG	1 cut(s)	383
NdeII	GATC	2 cut(s)	19, 397
NlaIII	CATG	2 cut(s)	113, 260
NlaIV	GGNNCC	1 cut(s)	558
NmuCI	GTSAC	2 cut(s)	40, 529
NspV	TTCGAA	1 cut(s)	470
PceI	AGGCCT	1 cut(s)	781
PdmI	GAANNNNTTC	1 cut(s)	834
PfeI	GAWTC	2 cut(s)	908, 936
PkrI	GCNGC	5 cut(s)	16, 245, 367, 684, 712
PleI	GAGTC	3 cut(s)	209, 257, 634
PpsI	GAGTC	3 cut(s)	209, 257, 634
Psp6I	CCWGG	2 cut(s)	92, 127
PspGI	CCWGG	2 cut(s)	92, 127
PspN4I	GGNNCC	1 cut(s)	558
PspPI	GGNCC	2 cut(s)	423, 557
PstI	CTGCAG	1 cut(s)	715
RsaI	GTAC	3 cut(s)	309, 461, 803
RsaNI	GTAC	3 cut(s)	308, 460, 802
SaqAI	TTAA	2 cut(s)	776, 903
SatI	GCNGC	5 cut(s)	15, 244, 366, 683, 711
Sau3AI	GATC	2 cut(s)	19, 397
Sau96I	GGNCC	2 cut(s)	423, 557
SchI	GAGTC	3 cut(s)	209, 258, 635
ScrFI	CCNGG	3 cut(s)	94, 129, 383
SfaNI	GCATC	2 cut(s)	179, 626
SfcI	CTRYAG	2 cut(s)	360, 711
SfuI	TTCGAA	1 cut(s)	470
SinI	GGWCC	1 cut(s)	423
SmlI	CTYRAG	5 cut(s)	161, 311, 521, 623, 775
SmoI	CTYRAG	5 cut(s)	161, 311, 521, 623, 775
Sse9I	AATT	6 cut(s)	580, 640, 733, 744, 833, 857
SseBI	AGGCCT	1 cut(s)	781
SsiI	CCGC	4 cut(s)	5, 14, 246, 366
StuI	AGGCCT	1 cut(s)	781
StyD4I	CCNGG	3 cut(s)	92, 127, 381
StyI	CCWWGG	1 cut(s)	630
TaaI	ACNGT	3 cut(s)	118, 189, 535
TaiI	ACGT	1 cut(s)	653
TaqI	TCGA	1 cut(s)	470
TasI	AATT	6 cut(s)	580, 640, 733, 744, 833, 857
TatI	WGTACW	1 cut(s)	307
TauI	GCSGC	2 cut(s)	17, 368
TfiI	GAWTC	2 cut(s)	908, 936
Tru1I	TTAA	2 cut(s)	776, 903
Tru9I	TTAA	2 cut(s)	776, 903
TscAI	CASTG	1 cut(s)	180
TseFI	GTSAC	2 cut(s)	40, 529
TseI	GCWGC	3 cut(s)	243, 682, 710
Tsp45I	GTSAC	2 cut(s)	40, 529
TspDTI	ATGAA	2 cut(s)	98, 392
TspGWI	ACGGA	1 cut(s)	415
TspRI	CASTG	1 cut(s)	180
Vha464I	CTTAAG	1 cut(s)	775
VpaK11BI	GGWCC	1 cut(s)	423
XapI	RAATTY	1 cut(s)	833
XmnI	GAANNNNTTC	1 cut(s)	834

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.