Rh7CG321500

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0001551
Physical Location & Seq
Forward (+)
34752759 .. 34755556
2798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG321500.1

Sequence Viewer

Length: 723 bp
ATGGCGGGTGAGAACGAAAAAGTATGCGTAACTGGAGCTGGAGGATTTGCTGCCTCTTGGGTCGTCAAGCTTCTTCTCTCTCAGGACTATCTTGTCCATGGAACCGTCAGACAACCAGGTGATAGCAAGTACGACCACTTGAACAGGCTTGAGAAAGCATCAGAGAACCTGAAACTGATCAAGGCAGATTTGCTGGACTATGATTCTCTATGTTCCGCAATTTCGGGATGCAGCGGTGTGTTCCATGTTGCCAGTCCTGTACCAACAACCGAAGTAACAAACCCCGAAGCAGAAGTAGTTGAACCAGCTGTGAAGGGAACGCTTAATGTGCTGAAAGCTTGCTTGGAGAACAAGGTGAAGAGAGTTGTTGTTGTGTCTTCTGTAGCTGCTGTTGTGATGAACCCGAACTGGCCCAAGGATAAAGTCAAGGATGAAAGCTGTTGGTCTGATCCTGAATATATTAAAACCACTAGTCTTGAATCCCTGAAAACTTCTAAGAAATGGTATTATATTTCGAAAACACAAGCAGAGAGCGAGGCTCTGGAGTTTGGAAAAAGAACTGGACTTGAGGTTGTTACTGTTTGTCCTTCTGCCATTTTGGGTCCACTTTTGCAATCTACCCTAAATTCCAGTACCTTGCTCCTGCCTATGGGTCTCAAAGCAAGAGAACATTATCCGATGTACATACGAAGTTTGTTCAGTAATTGTGAAATGTGCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

240

Amino Acids

26.25

Weight (kDa)

6.44

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 138 6.2e-08 NmrA-like family
Epimerase PF01370 8 - 205 1.5e-20 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 8 - 131 9e-09 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 10 - 208 8.4e-18 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 127 3.9e-13 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 205 7.7e-11 Male sterility protein
KR PF08659 10 - 135 1.3e-06 KR domain
NAD_binding_10 PF13460 12 - 132 6.1e-13 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AciI CCGC 3 cut(s) 5, 216, 234
AclWI GGATC 1 cut(s) 443
AcsI RAATTY 1 cut(s) 625
AfaI GTAC 4 cut(s) 131, 261, 634, 683
AfiI CCNNNNNNNGG 1 cut(s) 649
AgsI TTSAA 3 cut(s) 142, 302, 479
AhlI ACTAGT 1 cut(s) 470
AjnI CCWGG 1 cut(s) 115
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 6 cut(s) 38, 70, 308, 338, 386, 438
AluI AGCT 6 cut(s) 38, 70, 308, 338, 386, 438
Alw26I GTCTC 1 cut(s) 659
AlwI GGATC 1 cut(s) 443
AoxI GGCC 1 cut(s) 410
ApeKI GCWGC 3 cut(s) 50, 231, 386
ApoI RAATTY 1 cut(s) 625
AspS9I GGNCC 2 cut(s) 411, 602
AsuHPI GGTGA 3 cut(s) 20, 131, 367
AsuII TTCGAA 1 cut(s) 515
AvaII GGWCC 1 cut(s) 602
BbsI GAAGAC 1 cut(s) 369
BbvI GCAGC 3 cut(s) 37, 243, 373
BciT130I CCWGG 1 cut(s) 117
BclI TGATCA 1 cut(s) 177
BcoDI GTCTC 1 cut(s) 659
BcuI ACTAGT 1 cut(s) 470
BfaI CTAG 1 cut(s) 471
BfmI CTRYAG 1 cut(s) 381
BisI GCNGC 3 cut(s) 51, 232, 387
BlsI GCNGC 3 cut(s) 52, 233, 388
Bme1390I CCNGG 1 cut(s) 117
Bme18I GGWCC 1 cut(s) 602
BmgT120I GGNCC 2 cut(s) 411, 602
BmiI GGNNCC 2 cut(s) 103, 603
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 2 cut(s) 167, 218
BpiI GAAGAC 1 cut(s) 369
BplI GAGNNNNNCTC 2 cut(s) 523, 555
BpmI CTGGAG 3 cut(s) 54, 60, 563
Bpu14I TTCGAA 1 cut(s) 515
BpuEI CTTGAG 2 cut(s) 170, 587
BsaI GGTCTC 1 cut(s) 659
BsaJI CCNNGG 2 cut(s) 97, 414
Bsc4I CCNNNNNNNGG 1 cut(s) 649
Bse1I ACTGG 5 cut(s) 37, 252, 413, 565, 630
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 2 cut(s) 97, 414
BseGI GGATG 2 cut(s) 233, 436
BseLI CCNNNNNNNGG 1 cut(s) 649
BseMII CTCAG 1 cut(s) 95
BseNI ACTGG 5 cut(s) 37, 252, 413, 565, 630
BseXI GCAGC 3 cut(s) 37, 243, 373
BshFI GGCC 1 cut(s) 412
BslI CCNNNNNNNGG 1 cut(s) 649
BsmAI GTCTC 1 cut(s) 659
BsnI GGCC 1 cut(s) 412
Bso31I GGTCTC 1 cut(s) 659
Bsp119I TTCGAA 1 cut(s) 515
Bsp1407I TGTACA 1 cut(s) 681
Bsp143I GATC 2 cut(s) 177, 448
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 3 cut(s) 5, 216, 234
BspANI GGCC 1 cut(s) 412
BspCNI CTCAG 1 cut(s) 94
BspLI GGNNCC 2 cut(s) 103, 603
BspPI GGATC 1 cut(s) 443
BspT104I TTCGAA 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 659
BsrGI TGTACA 1 cut(s) 681
BsrI ACTGG 5 cut(s) 37, 252, 413, 565, 630
BssECI CCNNGG 2 cut(s) 97, 414
BssMI GATC 2 cut(s) 177, 448
BssT1I CCWWGG 2 cut(s) 97, 414
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 2 cut(s) 106, 580
Bst6I CTCTTC 1 cut(s) 353
BstAUI TGTACA 1 cut(s) 681
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 1 cut(s) 340
BstDEI CTNAG 2 cut(s) 81, 495
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 2 cut(s) 233, 436
BstKTI GATC 2 cut(s) 180, 451
BstMAI GTCTC 1 cut(s) 659
BstMBI GATC 2 cut(s) 177, 448
BstMWI GCNNNNNNNGC 1 cut(s) 328
BstNI CCWGG 1 cut(s) 117
BstSCI CCNGG 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 381
BstV1I GCAGC 3 cut(s) 37, 243, 373
BstV2I GAAGAC 1 cut(s) 369
BsuRI GGCC 1 cut(s) 412
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 2 cut(s) 233, 436
Cac8I GCNNGC 1 cut(s) 340
Cfr13I GGNCC 2 cut(s) 411, 602
CsiI ACCWGGT 1 cut(s) 115
Csp6I GTAC 4 cut(s) 130, 260, 633, 682
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviAII CATG 2 cut(s) 98, 245
CviJI RGCY 9 cut(s) 38, 70, 148, 308, 338, 386, 412, 438, 539
CviKI_1 RGCY 9 cut(s) 38, 70, 148, 308, 338, 386, 412, 438, 539
CviQI GTAC 4 cut(s) 130, 260, 633, 682
DdeI CTNAG 2 cut(s) 81, 495
DpnI GATC 2 cut(s) 179, 450
DpnII GATC 2 cut(s) 177, 448
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
Eco130I CCWWGG 2 cut(s) 97, 414
Eco31I GGTCTC 1 cut(s) 659
Eco47I GGWCC 1 cut(s) 602
EcoRII CCWGG 1 cut(s) 115
EcoT14I CCWWGG 2 cut(s) 97, 414
ErhI CCWWGG 2 cut(s) 97, 414
FaeI CATG 2 cut(s) 101, 248
FaiI YATR 9 cut(s) 25, 99, 201, 211, 246, 459, 510, 650, 686
FatI CATG 2 cut(s) 97, 244
FbaI TGATCA 1 cut(s) 177
Fnu4HI GCNGC 3 cut(s) 51, 232, 387
FokI GGATG 2 cut(s) 240, 443
Fsp4HI GCNGC 3 cut(s) 51, 232, 387
FspBI CTAG 1 cut(s) 471
GluI GCNGC 3 cut(s) 51, 232, 387
GsuI CTGGAG 3 cut(s) 54, 60, 563
HaeIII GGCC 1 cut(s) 412
Hin1II CATG 2 cut(s) 101, 248
HindIII AAGCTT 2 cut(s) 68, 336
HinfI GANTC 2 cut(s) 203, 479
HphI GGTGA 3 cut(s) 20, 131, 367
Hpy166II GTNNAC 1 cut(s) 605
Hpy188I TCNGA 4 cut(s) 110, 163, 448, 678
Hpy188III TCNNGA 5 cut(s) 83, 225, 452, 476, 542
Hpy8I GTNNAC 1 cut(s) 605
HpyAV CCTTC 2 cut(s) 307, 597
HpyCH4III ACNGT 2 cut(s) 106, 580
HpyCH4V TGCA 2 cut(s) 231, 613
HpyF10VI GCNNNNNNNGC 1 cut(s) 328
HpyF3I CTNAG 2 cut(s) 81, 495
Hsp92II CATG 2 cut(s) 101, 248
Ksp22I TGATCA 1 cut(s) 177
Kzo9I GATC 2 cut(s) 177, 448
LmnI GCTCC 2 cut(s) 35, 645
Lsp1109I GCAGC 3 cut(s) 37, 243, 373
LweI GCATC 2 cut(s) 167, 218
MabI ACCWGGT 1 cut(s) 115
MaeI CTAG 1 cut(s) 471
MaeIII GTNAC 3 cut(s) 28, 274, 574
MalI GATC 2 cut(s) 179, 450
MboI GATC 2 cut(s) 177, 448
MboII GAAGA 3 cut(s) 65, 369, 370
MluCI AATT 3 cut(s) 219, 625, 703
MnlI CCTC 4 cut(s) 35, 64, 529, 562
MseI TTAA 2 cut(s) 324, 462
MspA1I CMGCKG 2 cut(s) 234, 308
MspR9I CCNGG 1 cut(s) 117
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 1 cut(s) 328
NcoI CCATGG 1 cut(s) 97
NdeII GATC 2 cut(s) 177, 448
NlaIII CATG 2 cut(s) 101, 248
NlaIV GGNNCC 2 cut(s) 103, 603
NspV TTCGAA 1 cut(s) 515
PfeI GAWTC 2 cut(s) 203, 479
PkrI GCNGC 3 cut(s) 52, 233, 388
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 2 cut(s) 103, 603
PspPI GGNCC 2 cut(s) 411, 602
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 4 cut(s) 131, 261, 634, 683
RsaNI GTAC 4 cut(s) 130, 260, 633, 682
SaqAI TTAA 2 cut(s) 324, 462
SatI GCNGC 3 cut(s) 51, 232, 387
Sau3AI GATC 2 cut(s) 177, 448
Sau96I GGNCC 2 cut(s) 411, 602
ScrFI CCNGG 1 cut(s) 117
SexAI ACCWGGT 1 cut(s) 115
SfaNI GCATC 2 cut(s) 167, 218
SfcI CTRYAG 1 cut(s) 381
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 1 cut(s) 602
SmlI CTYRAG 2 cut(s) 149, 566
SmoI CTYRAG 2 cut(s) 149, 566
SpeI ACTAGT 1 cut(s) 470
Sse9I AATT 3 cut(s) 219, 625, 703
SsiI CCGC 3 cut(s) 5, 216, 234
SspMI CTAG 1 cut(s) 471
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 2 cut(s) 97, 414
TaaI ACNGT 2 cut(s) 106, 580
TaqI TCGA 1 cut(s) 515
TasI AATT 3 cut(s) 219, 625, 703
TatI WGTACW 1 cut(s) 681
TfiI GAWTC 2 cut(s) 203, 479
Tru1I TTAA 2 cut(s) 324, 462
Tru9I TTAA 2 cut(s) 324, 462
TseI GCWGC 3 cut(s) 50, 231, 386
TspDTI ATGAA 2 cut(s) 413, 447
VpaK11BI GGWCC 1 cut(s) 602
XapI RAATTY 1 cut(s) 625
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.