RchiOBHm_Chr7g0216801

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001551
Physical Location & Seq
Reverse (-)
34500792 .. 34502310
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19400

Sequence Viewer

Length: 996 bp
ATGGTGGATGCGTATGAAACAGTATGTGTAACTGGAGCCGGAGGGTTTGCTGCCTCCTGGATTGTCAAGCTTCTACTCTCCAACAACTATGTCGTCCATGGAACCGTCAGACAACCAGGGGATAGCAAGTATGATCACTTGAACAAGCTGGAGAGATCACCGGAGAACCTGAAGCTGTTCAAGGCAGATTTGCTGGATTATGAGTCTCTGAGATTGGCGATTTCAGGATGCAGTGGAGTGTTCCACGTGGCCAGTCCTGTACCTTTTCCAACTGCCATAACAGACCCCGAAGCAGAAGTACTTAAACCAGCTGTGGAGGGAACCCTTAATGTATTGAAAGCTTGCGTAGAGGCCAAAGTGAAGAGAGTTGTTGTTGTGTCGTCTATAGCTGCTGTTGTAAAGAACCCGAATTGGCCCAAGGATCAAGTGAAAGACGAGAGATGTTGGTCTGATCCGGAATATGTTAGGTCCACTAAGAATTGGTATTATCTTTCCAAAACACAAGCAGAAGCTGCAGCCTTGGAGTTTGGAAGAAGCAGTGGACTTGATGTTGTAACTGTTTGTCCTTCTCTTATCATGGGGCCACTGTTGCAATCCACTCTGAATACTAGTAGCTGGCTCCTGCCTATGGGATTCAAAGGTACAAGAGTAAACTTAGTAGTTTCGAATTTACAACGTAACGTGGGCTACAATTACTGGACGGTGGTGGATGCTCGTGATTTAGCTGATGCACTGCTTCTTGTATACAAGAAATCTGAGGCTGGAGAAAGATACATATGCACAGCACATGCAATTGGGGTAGAAGAAGTAGTGGAGAAGTACTTGAAGATTTCACATCCCAACTACAACTATTCTCCCGAGCTTACGCATACTAAGGAAGAAGAGGAGAAGCTGAGTTCGGAAAAATTGGAGAGGTTGGGTTGGAGTTACCGGCCATTGGGGGAAACTCTTATTGATTCCATTGAAAGCTACCAGAAGATTAGAGTCATGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

331

Amino Acids

36.91

Weight (kDa)

5.96

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 140 4.8e-08 NmrA-like family
Epimerase PF01370 8 - 257 2.9e-25 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 8 - 132 1.9e-07 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 10 - 204 6.3e-18 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 277 3.4e-16 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 207 1.9e-10 Male sterility protein
KR PF08659 10 - 136 8.3e-06 KR domain
NAD_binding_10 PF13460 12 - 190 1.8e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 744
AccIII TCCGGA 1 cut(s) 454
AclWI GGATC 2 cut(s) 429, 446
AcoI YGGCCR 2 cut(s) 249, 932
AcsI RAATTY 1 cut(s) 667
AcuI CTGAAG 1 cut(s) 191
AcvI CACGTG 1 cut(s) 247
AfaI GTAC 4 cut(s) 261, 300, 643, 821
AfiI CCNNNNNNNGG 2 cut(s) 628, 937
AgsI TTSAA 6 cut(s) 142, 181, 337, 637, 826, 965
AhlI ACTAGT 1 cut(s) 608
AjnI CCWGG 2 cut(s) 56, 115
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 2 cut(s) 429, 446
AlwNI CAGNNNCTG 1 cut(s) 512
Ama87I CYCGRG 1 cut(s) 857
Aor13HI TCCGGA 1 cut(s) 454
AoxI GGCC 5 cut(s) 249, 351, 413, 581, 932
ApeKI GCWGC 4 cut(s) 50, 389, 512, 515
ApoI RAATTY 1 cut(s) 667
Asp700I GAANNNNTTC 1 cut(s) 176
AspS9I GGNCC 3 cut(s) 414, 468, 581
AsuHPI GGTGA 1 cut(s) 150
AsuII TTCGAA 1 cut(s) 665
AvaI CYCGRG 1 cut(s) 857
AvaII GGWCC 1 cut(s) 468
BaeI ACNNNNGTAYC 2 cut(s) 633, 666
BalI TGGCCA 1 cut(s) 251
BarI GAAGNNNNNNTAC 2 cut(s) 282, 314
BauI CACGAG 1 cut(s) 714
BbrPI CACGTG 1 cut(s) 247
BbvI GCAGC 4 cut(s) 37, 376, 499, 527
BciT130I CCWGG 2 cut(s) 58, 117
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 210
BcuI ACTAGT 1 cut(s) 608
BfaI CTAG 1 cut(s) 609
BfmI CTRYAG 2 cut(s) 384, 513
BisI GCNGC 4 cut(s) 51, 390, 513, 516
BlsI GCNGC 4 cut(s) 52, 391, 514, 517
BmcAI AGTACT 2 cut(s) 300, 821
Bme1390I CCNGG 2 cut(s) 58, 117
Bme18I GGWCC 1 cut(s) 468
BmeT110I CYCGRG 1 cut(s) 857
BmgT120I GGNCC 3 cut(s) 414, 468, 581
BmiI GGNNCC 5 cut(s) 37, 103, 322, 582, 620
BmrFI CCNGG 2 cut(s) 58, 117
BmsI GCATC 3 cut(s) 218, 700, 718
BpmI CTGGAG 3 cut(s) 54, 170, 783
Bpu14I TTCGAA 1 cut(s) 665
BsaAI YACGTR 1 cut(s) 247
BsaJI CCNNGG 4 cut(s) 97, 116, 417, 519
BsaWI WCCGGW 2 cut(s) 160, 454
Bsc4I CCNNNNNNNGG 2 cut(s) 628, 937
Bse118I RCCGGY 1 cut(s) 930
Bse1I ACTGG 3 cut(s) 37, 252, 701
BseAI TCCGGA 1 cut(s) 454
BseBI CCWGG 2 cut(s) 58, 117
BseDI CCNNGG 4 cut(s) 97, 116, 417, 519
BseGI GGATG 4 cut(s) 13, 233, 715, 835
BseLI CCNNNNNNNGG 2 cut(s) 628, 937
BseMII CTCAG 3 cut(s) 200, 747, 884
BseNI ACTGG 3 cut(s) 37, 252, 701
BseRI GAGGAG 1 cut(s) 899
BseXI GCAGC 4 cut(s) 37, 376, 499, 527
BshFI GGCC 5 cut(s) 251, 353, 415, 583, 934
BsiHKCI CYCGRG 1 cut(s) 857
BsiSI CCGG 4 cut(s) 39, 161, 455, 931
BslI CCNNNNNNNGG 2 cut(s) 628, 937
BsmAI GTCTC 1 cut(s) 210
BsnI GGCC 5 cut(s) 251, 353, 415, 583, 934
BsoBI CYCGRG 1 cut(s) 857
Bsp119I TTCGAA 1 cut(s) 665
Bsp13I TCCGGA 1 cut(s) 454
Bsp143I GATC 4 cut(s) 133, 155, 421, 451
Bsp19I CCATGG 1 cut(s) 97
BspANI GGCC 5 cut(s) 251, 353, 415, 583, 934
BspCNI CTCAG 3 cut(s) 201, 748, 885
BspEI TCCGGA 1 cut(s) 454
BspLI GGNNCC 5 cut(s) 37, 103, 322, 582, 620
BspMAI CTGCAG 1 cut(s) 517
BspPI GGATC 2 cut(s) 429, 446
BspT104I TTCGAA 1 cut(s) 665
BsrFI RCCGGY 1 cut(s) 930
BsrI ACTGG 3 cut(s) 37, 252, 701
BssAI RCCGGY 1 cut(s) 930
BssECI CCNNGG 4 cut(s) 97, 116, 417, 519
BssMI GATC 4 cut(s) 133, 155, 421, 451
BssNAI GTATAC 1 cut(s) 745
BssSI CACGAG 1 cut(s) 714
BssT1I CCWWGG 3 cut(s) 97, 417, 519
Bst1107I GTATAC 1 cut(s) 745
Bst2BI CACGAG 1 cut(s) 714
Bst2UI CCWGG 2 cut(s) 58, 117
Bst4CI ACNGT 5 cut(s) 22, 106, 559, 588, 703
Bst6I CTCTTC 2 cut(s) 356, 876
BstAPI GCANNNNNTGC 1 cut(s) 512
BstBAI YACGTR 1 cut(s) 247
BstBI TTCGAA 1 cut(s) 665
BstC8I GCNNGC 2 cut(s) 343, 617
BstDEI CTNAG 6 cut(s) 209, 474, 655, 756, 873, 893
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 4 cut(s) 13, 233, 715, 835
BstKTI GATC 4 cut(s) 136, 158, 424, 454
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 4 cut(s) 133, 155, 421, 451
BstMWI GCNNNNNNNGC 2 cut(s) 512, 589
BstNI CCWGG 2 cut(s) 58, 117
BstNSI RCATGY 1 cut(s) 791
BstSCI CCNGG 2 cut(s) 56, 115
BstSFI CTRYAG 2 cut(s) 384, 513
BstV1I GCAGC 4 cut(s) 37, 376, 499, 527
BstZ17I GTATAC 1 cut(s) 745
BsuRI GGCC 5 cut(s) 251, 353, 415, 583, 934
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 4 cut(s) 13, 233, 715, 835
BtsI GCAGTG 3 cut(s) 238, 544, 731
BtsIMutI CAGTG 4 cut(s) 238, 544, 584, 731
Cac8I GCNNGC 2 cut(s) 343, 617
CaiI CAGNNNCTG 1 cut(s) 512
Cfr10I RCCGGY 1 cut(s) 930
Cfr13I GGNCC 3 cut(s) 414, 468, 581
Csp6I GTAC 4 cut(s) 260, 299, 642, 820
CviAII CATG 4 cut(s) 98, 577, 788, 988
CviQI GTAC 4 cut(s) 260, 299, 642, 820
DdeI CTNAG 6 cut(s) 209, 474, 655, 756, 873, 893
DpnI GATC 4 cut(s) 135, 157, 423, 453
DpnII GATC 4 cut(s) 133, 155, 421, 451
EaeI YGGCCR 2 cut(s) 249, 932
Eam1104I CTCTTC 2 cut(s) 356, 876
EarI CTCTTC 2 cut(s) 356, 876
Eco130I CCWWGG 3 cut(s) 97, 417, 519
Eco47I GGWCC 1 cut(s) 468
Eco57I CTGAAG 1 cut(s) 191
Eco72I CACGTG 1 cut(s) 247
Eco88I CYCGRG 1 cut(s) 857
EcoRII CCWGG 2 cut(s) 56, 115
EcoT14I CCWWGG 3 cut(s) 97, 417, 519
ErhI CCWWGG 3 cut(s) 97, 417, 519
FaeI CATG 4 cut(s) 101, 580, 791, 991
FatI CATG 4 cut(s) 97, 576, 787, 987
FauNDI CATATG 1 cut(s) 776
FbaI TGATCA 1 cut(s) 133
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 4 cut(s) 51, 390, 513, 516
FokI GGATG 4 cut(s) 20, 240, 722, 822
Fsp4HI GCNGC 4 cut(s) 51, 390, 513, 516
FspBI CTAG 1 cut(s) 609
GluI GCNGC 4 cut(s) 51, 390, 513, 516
GsuI CTGGAG 3 cut(s) 54, 170, 783
HaeIII GGCC 5 cut(s) 251, 353, 415, 583, 934
HapII CCGG 4 cut(s) 39, 161, 455, 931
Hin1II CATG 4 cut(s) 101, 580, 791, 991
HindIII AAGCTT 2 cut(s) 68, 339
HinfI GANTC 4 cut(s) 203, 633, 956, 984
HpaII CCGG 4 cut(s) 39, 161, 455, 931
HphI GGTGA 1 cut(s) 150
Hpy166II GTNNAC 4 cut(s) 471, 542, 652, 745
Hpy188I TCNGA 6 cut(s) 110, 210, 451, 603, 757, 901
Hpy188III TCNNGA 4 cut(s) 225, 455, 716, 857
Hpy8I GTNNAC 4 cut(s) 471, 542, 652, 745
HpyAV CCTTC 1 cut(s) 576
HpyCH4III ACNGT 5 cut(s) 22, 106, 559, 588, 703
HpyCH4IV ACGT 3 cut(s) 246, 676, 681
HpyCH4V TGCA 6 cut(s) 231, 515, 592, 731, 780, 791
HpyF10VI GCNNNNNNNGC 2 cut(s) 512, 589
HpyF3I CTNAG 6 cut(s) 209, 474, 655, 756, 873, 893
HpySE526I ACGT 3 cut(s) 246, 676, 681
Hsp92II CATG 4 cut(s) 101, 580, 791, 991
Kpn2I TCCGGA 1 cut(s) 454
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 4 cut(s) 133, 155, 421, 451
LmnI GCTCC 2 cut(s) 35, 624
Lsp1109I GCAGC 4 cut(s) 37, 376, 499, 527
LweI GCATC 3 cut(s) 218, 700, 718
MaeI CTAG 1 cut(s) 609
MaeII ACGT 3 cut(s) 246, 676, 681
MaeIII GTNAC 4 cut(s) 28, 553, 677, 926
MalI GATC 4 cut(s) 135, 157, 423, 453
MboI GATC 4 cut(s) 133, 155, 421, 451
MboII GAAGA 7 cut(s) 373, 543, 815, 838, 890, 893, 988
MfeI CAATTG 1 cut(s) 792
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 6 cut(s) 409, 478, 667, 691, 792, 905
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 2 cut(s) 212, 993
MmeI TCCRAC 3 cut(s) 105, 293, 902
MnlI CCTC 7 cut(s) 35, 64, 310, 343, 751, 877, 906
Mox20I TGGCCA 1 cut(s) 251
MroI TCCGGA 1 cut(s) 454
MroXI GAANNNNTTC 1 cut(s) 176
MscI TGGCCA 1 cut(s) 251
MseI TTAA 2 cut(s) 303, 327
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 311
MspI CCGG 4 cut(s) 39, 161, 455, 931
MspR9I CCNGG 2 cut(s) 58, 117
MunI CAATTG 1 cut(s) 792
MvaI CCWGG 2 cut(s) 58, 117
MwoI GCNNNNNNNGC 2 cut(s) 512, 589
NcoI CCATGG 1 cut(s) 97
NdeI CATATG 1 cut(s) 776
NdeII GATC 4 cut(s) 133, 155, 421, 451
NlaIII CATG 4 cut(s) 101, 580, 791, 991
NlaIV GGNNCC 5 cut(s) 37, 103, 322, 582, 620
NspI RCATGY 1 cut(s) 791
NspV TTCGAA 1 cut(s) 665
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 2 cut(s) 633, 956
PfoI TCCNGGA 1 cut(s) 56
PkrI GCNGC 4 cut(s) 52, 391, 514, 517
PleI GAGTC 2 cut(s) 211, 992
PmaCI CACGTG 1 cut(s) 247
PmlI CACGTG 1 cut(s) 247
PpsI GAGTC 2 cut(s) 211, 992
Ppu21I YACGTR 1 cut(s) 247
Psp6I CCWGG 2 cut(s) 56, 115
PspCI CACGTG 1 cut(s) 247
PspGI CCWGG 2 cut(s) 56, 115
PspN4I GGNNCC 5 cut(s) 37, 103, 322, 582, 620
PspPI GGNCC 3 cut(s) 414, 468, 581
PstI CTGCAG 1 cut(s) 517
PstNI CAGNNNCTG 1 cut(s) 512
PvuII CAGCTG 1 cut(s) 311
RsaI GTAC 4 cut(s) 261, 300, 643, 821
RsaNI GTAC 4 cut(s) 260, 299, 642, 820
SaqAI TTAA 2 cut(s) 303, 327
SatI GCNGC 4 cut(s) 51, 390, 513, 516
Sau3AI GATC 4 cut(s) 133, 155, 421, 451
Sau96I GGNCC 3 cut(s) 414, 468, 581
ScaI AGTACT 2 cut(s) 300, 821
SchI GAGTC 2 cut(s) 212, 993
ScrFI CCNGG 2 cut(s) 58, 117
SfaNI GCATC 3 cut(s) 218, 700, 718
SfcI CTRYAG 2 cut(s) 384, 513
SfuI TTCGAA 1 cut(s) 665
SinI GGWCC 1 cut(s) 468
SpeI ACTAGT 1 cut(s) 608
Sse9I AATT 6 cut(s) 409, 478, 667, 691, 792, 905
SspMI CTAG 1 cut(s) 609
StyD4I CCNGG 2 cut(s) 56, 115
StyI CCWWGG 3 cut(s) 97, 417, 519
TaaI ACNGT 5 cut(s) 22, 106, 559, 588, 703
TaiI ACGT 3 cut(s) 249, 679, 684
TaqI TCGA 1 cut(s) 665
TasI AATT 6 cut(s) 409, 478, 667, 691, 792, 905
TatI WGTACW 2 cut(s) 298, 819
TfiI GAWTC 2 cut(s) 633, 956
Tru1I TTAA 2 cut(s) 303, 327
Tru9I TTAA 2 cut(s) 303, 327
TscAI CASTG 4 cut(s) 238, 544, 591, 738
TseI GCWGC 4 cut(s) 50, 389, 512, 515
TspDTI ATGAA 1 cut(s) 30
TspRI CASTG 4 cut(s) 238, 544, 591, 738
VpaK11BI GGWCC 1 cut(s) 468
XapI RAATTY 1 cut(s) 667
XceI RCATGY 1 cut(s) 791
XmiI GTMKAC 1 cut(s) 744
XmnI GAANNNNTTC 1 cut(s) 176
XspI CTAG 1 cut(s) 609
ZrmI AGTACT 2 cut(s) 300, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.