Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001551

Physical Location & Seq

Genomic Coordinates Forward (+)

452881 .. 454913

Gene Structure 2033 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1003800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 933 bp

ATGGTGGGTGGGACGACTGATCAGAAAGAAAAAGTATGCGTGACTGGAGCTGGAGGATTTACTGGCTCTTGGCTCGTCAACCTCCTTCTCTCCAAGGACTATGTTGTTCATGGAACCGTCAGGCAACCAGACAGCAAGTATGATCACTTGAACAAGCTTGAGAAAGCATCGGAGAACCTCAAACTGTTCAAGGCAGATTTGTTGGACTACGACTCTCTTCGTTTGGCCATTGAAGGTTGCAGCGGAGTCTTCCATGAGTTTCTCGAACCAGCAATAAAGGGAACGCTGAATGTACTCAAGGCTTGTAAAGAAGCCAACGTGAAGAGAGTTGTAGTTGTGTCGTCTTTAGCCGCTGTTGTTATGAACCCGGAGTGGCCTAAGGATCAAGTCAAGGACGAGACTTGTTGGTCCGTTCCCGAATATATGAAAACAACCAAGAAATGGTACTATCTTTCGAAAACGGAAGCAGAACGCGAGGCTCTGGAGTTTGGAAAAAGGAATGGACTTGAGGTTGTGACTGTTTGTCCTTCTCTCATTTTGGGGCCAATTTTGCAATCCACTCGCAAATGCAGTAGCTTTCTCGTTGTTATGACTATAAAAGGTGATCTTGAGTCCTTGCCATGCAATTACTGGACGTTCGTGGATGTTCGTGATTTAGCTGAAGCGCTGCTTCTTGCATACAAGAAGTCTGAGGCTGCAGGAGAAAGATACATATGCCATTCACACTCAATTGGCATACGAGATGTGGTGGAGAAATACTTAAGGCCTACATATCCCGATTACAAGTACCCCAAGAACCTTACCTACGCAGAGGAAGAAGTACAACACATTAATTCCGAAAAATTGCAGAAGTTGGGTTGGACTTTCCGGCCAGTTGAGGAAACGCTTAATGATTCTATTGAAAGCCACCGGAAGGCTGGAATCGTGGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Epimerase

Protein Analysis

311

Amino Acids

34.98

Weight (kDa)

6.67

Isoelectric Point (pI)

39.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NmrA	PF05368	8 - 128	1.3e-10	NmrA-like family
Epimerase	PF01370	12 - 237	3.4e-21	NAD dependent epimerase/dehydratase family
3Beta_HSD	PF01073	14 - 189	4e-14	3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd	PF16363	14 - 80	2.2e-11	GDP-mannose 4,6 dehydratase
NAD_binding_10	PF13460	16 - 176	1.4e-16	NAD(P)H-binding
NAD_binding_4	PF07993	85 - 186	4.8e-07	Male sterility protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001551)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76470
fragaria_vesca	FvH4_5g26030 FvH4_5g26030 FvH4_5g26090
malus_domestica	MD06G1003300.v1.1 MD06G1003700.v1.1 MD06G1003800.v1.1 MD06G1003900.v1.1
prunus_persica	Prupe.5G004900_v2.0.a1 Prupe.5G005000_v2.0.a1
pyrus_communis	pycom06g00340 pycom06g00350 pycom06g00360 pycom06g00380
rosa_chinensis	RchiOBHm_Chr7g0216711 RchiOBHm_Chr7g0216751 RchiOBHm_Chr7g0216801
rosa_laevigata	RLG00000002541 RLG00000002544
rosa_multiflora	Rmu_co8327343.1_g000001 Rmu_sc0000125.1_g000013 Rmu_sc0003814.1_g000008 Rmu_sc0003814.1_g000009 Rmu_sc0004941.1_g000004 Rmu_ssc0000117.1_g000016 Rmu_ssc0000117.1_g000017 Rmu_ssc0000375.1_g000001
rosa_roxburghii	Rroxscaffold_3G00242850 Rroxscaffold_3G00242960 Rroxscaffold_3G00242980 Rroxscaffold_5G00345210 Rroxscaffold_7G00166270
rosa_rugosa	Rorug07G0161900 Rorug07G0162100
rosa_samantha	Rh7AG301800 Rh7AG302000 Rh7AG302900 Rh7BG293600 Rh7BG293800 Rh7BG294600 Rh7CG320500 Rh7CG320800 Rh7CG321500 Rh7DG302500 Rh7DG302900 Rh7DG303100 Rh7DG303200
rosa_wichuraiana	Rw0G009080 Rw7G025620 Rw7G025730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	406
AccB7I	CCANNNNNTGG	1 cut(s)	441
AccII	CGCG	1 cut(s)	474
AciI	CCGC	2 cut(s)	243, 351
AclWI	GGATC	1 cut(s)	390
AcoI	YGGCCR	2 cut(s)	225, 869
AcuI	CTGAAG	1 cut(s)	681
AfaI	GTAC	4 cut(s)	294, 446, 788, 822
AfeI	AGCGCT	1 cut(s)	666
AfiI	CCNNNNNNNGG	2 cut(s)	441, 913
AflII	CTTAAG	1 cut(s)	760
AgsI	TTSAA	4 cut(s)	151, 190, 233, 902
AhdI	GACNNNNNGTC	1 cut(s)	522
AluBI	AGCT	4 cut(s)	50, 157, 576, 659
AluI	AGCT	4 cut(s)	50, 157, 576, 659
Alw26I	GTCTC	1 cut(s)	392
AlwI	GGATC	1 cut(s)	390
Aor51HI	AGCGCT	1 cut(s)	666
AoxI	GGCC	5 cut(s)	225, 374, 542, 764, 869
ApeKI	GCWGC	3 cut(s)	240, 667, 695
AseI	ATTAAT	1 cut(s)	831
AspLEI	GCGC	1 cut(s)	667
AspS9I	GGNCC	2 cut(s)	408, 542
AsuC2I	CCSGG	1 cut(s)	368
AsuHPI	GGTGA	1 cut(s)	614
AsuII	TTCGAA	1 cut(s)	455
AvaII	GGWCC	1 cut(s)	408
AxyI	CCTNAGG	1 cut(s)	378
BalI	TGGCCA	1 cut(s)	227
BbsI	GAAGAC	1 cut(s)	241
BbvI	GCAGC	3 cut(s)	252, 654, 682
BcgI	CGANNNNNNTGC	2 cut(s)	542, 576
BclI	TGATCA	2 cut(s)	19, 142
BcnI	CCSGG	1 cut(s)	368
BcoDI	GTCTC	1 cut(s)	392
BfmI	CTRYAG	1 cut(s)	696
BfoI	RGCGCY	1 cut(s)	668
BfrI	CTTAAG	1 cut(s)	760
BisI	GCNGC	4 cut(s)	241, 351, 668, 696
BlsI	GCNGC	4 cut(s)	242, 352, 669, 697
Bme1390I	CCNGG	1 cut(s)	368
Bme18I	GGWCC	1 cut(s)	408
BmeRI	GACNNNNNGTC	1 cut(s)	522
BmgT120I	GGNCC	2 cut(s)	408, 542
BmiI	GGNNCC	2 cut(s)	115, 543
BmrFI	CCNGG	1 cut(s)	368
BmsI	GCATC	1 cut(s)	176
BpiI	GAAGAC	1 cut(s)	241
BpmI	CTGGAG	3 cut(s)	66, 72, 503
Bpu14I	TTCGAA	1 cut(s)	455
BpuEI	CTTGAG	4 cut(s)	179, 281, 527, 629
BpuMI	CCSGG	1 cut(s)	368
BsaJI	CCNNGG	1 cut(s)	93
BsaWI	WCCGGW	1 cut(s)	909
Bsc4I	CCNNNNNNNGG	2 cut(s)	441, 913
Bse1I	ACTGG	4 cut(s)	49, 67, 635, 872
Bse21I	CCTNAGG	1 cut(s)	378
BseDI	CCNNGG	1 cut(s)	93
BseGI	GGATG	1 cut(s)	649
BseLI	CCNNNNNNNGG	2 cut(s)	441, 913
BseMII	CTCAG	1 cut(s)	681
BseNI	ACTGG	4 cut(s)	49, 67, 635, 872
BseXI	GCAGC	3 cut(s)	252, 654, 682
Bsh1236I	CGCG	1 cut(s)	474
BshFI	GGCC	5 cut(s)	227, 376, 544, 766, 871
BsiSI	CCGG	3 cut(s)	368, 868, 910
BslFI	GGGAC	1 cut(s)	25
BslI	CCNNNNNNNGG	2 cut(s)	441, 913
BsmAI	GTCTC	1 cut(s)	392
BsmFI	GGGAC	1 cut(s)	25
BsnI	GGCC	5 cut(s)	227, 376, 544, 766, 871
Bsp119I	TTCGAA	1 cut(s)	455
Bsp143I	GATC	4 cut(s)	19, 142, 382, 604
BspACI	CCGC	2 cut(s)	243, 351
BspANI	GGCC	5 cut(s)	227, 376, 544, 766, 871
BspCNI	CTCAG	1 cut(s)	682
BspFNI	CGCG	1 cut(s)	474
BspLI	GGNNCC	2 cut(s)	115, 543
BspMAI	CTGCAG	1 cut(s)	700
BspPI	GGATC	1 cut(s)	390
BspT104I	TTCGAA	1 cut(s)	455
BspTI	CTTAAG	1 cut(s)	760
BsrI	ACTGG	4 cut(s)	49, 67, 635, 872
BssECI	CCNNGG	1 cut(s)	93
BssMI	GATC	4 cut(s)	19, 142, 382, 604
BssT1I	CCWWGG	1 cut(s)	93
Bst4CI	ACNGT	3 cut(s)	118, 186, 520
Bst6I	CTCTTC	2 cut(s)	222, 317
BstAFI	CTTAAG	1 cut(s)	760
BstBI	TTCGAA	1 cut(s)	455
BstDEI	CTNAG	2 cut(s)	378, 690
BstF5I	GGATG	1 cut(s)	649
BstFNI	CGCG	1 cut(s)	474
BstH2I	RGCGCY	1 cut(s)	668
BstHHI	GCGC	1 cut(s)	667
BstKTI	GATC	4 cut(s)	22, 145, 385, 607
BstMAI	GTCTC	1 cut(s)	392
BstMBI	GATC	4 cut(s)	19, 142, 382, 604
BstMWI	GCNNNNNNNGC	1 cut(s)	550
BstSCI	CCNGG	1 cut(s)	366
BstSFI	CTRYAG	1 cut(s)	696
BstUI	CGCG	1 cut(s)	474
BstV1I	GCAGC	3 cut(s)	252, 654, 682
BstV2I	GAAGAC	1 cut(s)	241
Bsu36I	CCTNAGG	1 cut(s)	378
BsuRI	GGCC	5 cut(s)	227, 376, 544, 766, 871
BtsCI	GGATG	1 cut(s)	649
CfoI	GCGC	1 cut(s)	667
Cfr13I	GGNCC	2 cut(s)	408, 542
Csp6I	GTAC	4 cut(s)	293, 445, 787, 821
CviAII	CATG	3 cut(s)	110, 254, 621
CviQI	GTAC	4 cut(s)	293, 445, 787, 821
DdeI	CTNAG	2 cut(s)	378, 690
DpnI	GATC	4 cut(s)	21, 144, 384, 606
DpnII	GATC	4 cut(s)	19, 142, 382, 604
DrdI	GACNNNNNNGTC	1 cut(s)	406
DriI	GACNNNNNGTC	1 cut(s)	522
DseDI	GACNNNNNNGTC	1 cut(s)	406
EaeI	YGGCCR	2 cut(s)	225, 869
Eam1104I	CTCTTC	2 cut(s)	222, 317
Eam1105I	GACNNNNNGTC	1 cut(s)	522
EarI	CTCTTC	2 cut(s)	222, 317
Eco130I	CCWWGG	1 cut(s)	93
Eco147I	AGGCCT	1 cut(s)	766
Eco47I	GGWCC	1 cut(s)	408
Eco47III	AGCGCT	1 cut(s)	666
Eco57I	CTGAAG	1 cut(s)	681
Eco81I	CCTNAGG	1 cut(s)	378
EcoT14I	CCWWGG	1 cut(s)	93
ErhI	CCWWGG	1 cut(s)	93
FaeI	CATG	3 cut(s)	113, 257, 624
FalI	AAGNNNNNCTT	4 cut(s)	591, 623, 654, 686
FaqI	GGGAC	1 cut(s)	25
FatI	CATG	3 cut(s)	109, 253, 620
FauNDI	CATATG	1 cut(s)	713
FbaI	TGATCA	2 cut(s)	19, 142
Fnu4HI	GCNGC	4 cut(s)	241, 351, 668, 696
FokI	GGATG	1 cut(s)	656
Fsp4HI	GCNGC	4 cut(s)	241, 351, 668, 696
GlaI	GCGC	1 cut(s)	666
GluI	GCNGC	4 cut(s)	241, 351, 668, 696
GsuI	CTGGAG	3 cut(s)	66, 72, 503
HaeII	RGCGCY	1 cut(s)	668
HaeIII	GGCC	5 cut(s)	227, 376, 544, 766, 871
HapII	CCGG	3 cut(s)	368, 868, 910
HhaI	GCGC	1 cut(s)	667
Hin1II	CATG	3 cut(s)	113, 257, 624
Hin6I	GCGC	1 cut(s)	665
HinP1I	GCGC	1 cut(s)	665
HincII	GTYRAC	1 cut(s)	79
HindII	GTYRAC	1 cut(s)	79
HindIII	AAGCTT	1 cut(s)	155
HinfI	GANTC	5 cut(s)	212, 246, 611, 893, 921
HpaII	CCGG	3 cut(s)	368, 868, 910
HphI	GGTGA	1 cut(s)	614
Hpy166II	GTNNAC	1 cut(s)	79
Hpy188I	TCNGA	4 cut(s)	24, 172, 691, 838
Hpy188III	TCNNGA	6 cut(s)	263, 416, 482, 608, 650, 776
Hpy8I	GTNNAC	1 cut(s)	79
HpyAV	CCTTC	4 cut(s)	95, 227, 537, 907
HpyCH4III	ACNGT	3 cut(s)	118, 186, 520
HpyCH4IV	ACGT	2 cut(s)	318, 635
HpyCH4V	TGCA	7 cut(s)	240, 553, 570, 624, 677, 698, 847
HpyF10VI	GCNNNNNNNGC	1 cut(s)	550
HpyF3I	CTNAG	2 cut(s)	378, 690
HpySE526I	ACGT	2 cut(s)	318, 635
Hsp92II	CATG	3 cut(s)	113, 257, 624
HspAI	GCGC	1 cut(s)	665
Ksp22I	TGATCA	2 cut(s)	19, 142
Kzo9I	GATC	4 cut(s)	19, 142, 382, 604
LmnI	GCTCC	1 cut(s)	47
Lsp1109I	GCAGC	3 cut(s)	252, 654, 682
LweI	GCATC	1 cut(s)	176
MaeII	ACGT	2 cut(s)	318, 635
MaeIII	GTNAC	2 cut(s)	40, 514
MalI	GATC	4 cut(s)	21, 144, 384, 606
MboI	GATC	4 cut(s)	19, 142, 382, 604
MboII	GAAGA	4 cut(s)	209, 241, 334, 827
MfeI	CAATTG	1 cut(s)	729
MlsI	TGGCCA	1 cut(s)	227
MluCI	AATT	5 cut(s)	546, 625, 729, 832, 842
MluNI	TGGCCA	1 cut(s)	227
MlyI	GAGTC	3 cut(s)	206, 255, 620
MmeI	TCCRAC	2 cut(s)	183, 839
MnlI	CCTC	8 cut(s)	47, 92, 188, 469, 502, 685, 805, 871
Mox20I	TGGCCA	1 cut(s)	227
MscI	TGGCCA	1 cut(s)	227
MseI	TTAA	3 cut(s)	761, 831, 888
Msp20I	TGGCCA	1 cut(s)	227
MspA1I	CMGCKG	2 cut(s)	243, 353
MspCI	CTTAAG	1 cut(s)	760
MspI	CCGG	3 cut(s)	368, 868, 910
MspR9I	CCNGG	1 cut(s)	368
MunI	CAATTG	1 cut(s)	729
MvnI	CGCG	1 cut(s)	474
MwoI	GCNNNNNNNGC	1 cut(s)	550
NciI	CCSGG	1 cut(s)	368
NdeI	CATATG	1 cut(s)	713
NdeII	GATC	4 cut(s)	19, 142, 382, 604
NlaIII	CATG	3 cut(s)	113, 257, 624
NlaIV	GGNNCC	2 cut(s)	115, 543
NmuCI	GTSAC	2 cut(s)	40, 514
NspV	TTCGAA	1 cut(s)	455
PceI	AGGCCT	1 cut(s)	766
PfeI	GAWTC	2 cut(s)	893, 921
PflMI	CCANNNNNTGG	1 cut(s)	441
PkrI	GCNGC	4 cut(s)	242, 352, 669, 697
PleI	GAGTC	3 cut(s)	206, 254, 619
PpsI	GAGTC	3 cut(s)	206, 254, 619
PshBI	ATTAAT	1 cut(s)	831
PspN4I	GGNNCC	2 cut(s)	115, 543
PspPI	GGNCC	2 cut(s)	408, 542
PsrI	GAACNNNNNNTAC	2 cut(s)	788, 820
PstI	CTGCAG	1 cut(s)	700
RsaI	GTAC	4 cut(s)	294, 446, 788, 822
RsaNI	GTAC	4 cut(s)	293, 445, 787, 821
SaqAI	TTAA	3 cut(s)	761, 831, 888
SatI	GCNGC	4 cut(s)	241, 351, 668, 696
Sau3AI	GATC	4 cut(s)	19, 142, 382, 604
Sau96I	GGNCC	2 cut(s)	408, 542
SchI	GAGTC	3 cut(s)	206, 255, 620
ScrFI	CCNGG	1 cut(s)	368
SfaNI	GCATC	1 cut(s)	176
SfcI	CTRYAG	1 cut(s)	696
SfuI	TTCGAA	1 cut(s)	455
SinI	GGWCC	1 cut(s)	408
SmlI	CTYRAG	5 cut(s)	158, 296, 506, 608, 760
SmoI	CTYRAG	5 cut(s)	158, 296, 506, 608, 760
Sse9I	AATT	5 cut(s)	546, 625, 729, 832, 842
SseBI	AGGCCT	1 cut(s)	766
SsiI	CCGC	2 cut(s)	243, 351
StuI	AGGCCT	1 cut(s)	766
StyD4I	CCNGG	1 cut(s)	366
StyI	CCWWGG	1 cut(s)	93
TaaI	ACNGT	3 cut(s)	118, 186, 520
TaiI	ACGT	2 cut(s)	321, 638
TaqI	TCGA	2 cut(s)	264, 455
TasI	AATT	5 cut(s)	546, 625, 729, 832, 842
TatI	WGTACW	2 cut(s)	292, 820
TauI	GCSGC	1 cut(s)	353
TfiI	GAWTC	2 cut(s)	893, 921
Tru1I	TTAA	3 cut(s)	761, 831, 888
Tru9I	TTAA	3 cut(s)	761, 831, 888
TseFI	GTSAC	2 cut(s)	40, 514
TseI	GCWGC	3 cut(s)	240, 667, 695
Tsp45I	GTSAC	2 cut(s)	40, 514
TspDTI	ATGAA	3 cut(s)	98, 377, 440
TspGWI	ACGGA	2 cut(s)	400, 476
Van91I	CCANNNNNTGG	1 cut(s)	441
Vha464I	CTTAAG	1 cut(s)	760
VpaK11BI	GGWCC	1 cut(s)	408
VspI	ATTAAT	1 cut(s)	831
XcmI	CCANNNNNNNNNTGG	2 cut(s)	627, 914

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.