Rw7G025620

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001551
Physical Location & Seq
Reverse (-)
32072590 .. 32074696
2107 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025620.1

Sequence Viewer

Length: 981 bp
ATGGGAGAGAAAGATACAGTGTGTGTAACTGGTGCCGGAGGCTTTGTGGCATCTTGGGTCGTCAAGCTGCTTCTCTCTCAAGGATACAAGGTTCATGGCACCGCTAGAGACCCAGGTGATGAGAAGAATGCCCATTTGAAGAAACTGGAAAACGCTTCTGAGAATTTGCAACTGTTCAAGGCAGACTTGTTCGACGTTGAAGGTCTTTGTAATGCCATTTCTGGGTGTTCTGGTGTTTTCCATGTTGCCTGCCCTGTTCCATCGGACGAAGTTTCTAACCCTGATGAGAGAGAATTTATCGAACCTGCTGTAAGGGGCACACGGAATGTACTAGATGCATGTATCAAGGCAAAAGTAAAGAGGGTCGTGGTGGTTTCATCTGCTGGTGCTGTTATATACAACCCAAAATGGCCCAAGGATAAACCCATGGATGAAGAATGTTGGTCTGACCCAGAGTATTGCAAGAAAGCTAAGACCCAGTTTGGCAACTATTGTCTTGCCAAGACAATGGCAGAAACTGAAGCTTTGGAGTATGCAAAGAAAAGTGAATTGAGAATCATTACTGTTTGTCCATCCTTTGTTTTTGGTCCAATGCTGCAATCAACATTAAATGCCAGCAACTCATTGCTCATAACTCTTCTAAAAGATGGACTGGAATCTGTTGAGAACCGTGCTCGTCCTGTTGTTGATGTGCGTGATGTAGCTGAAGCTCTACTTCTGGCATATGAAGAGCCAGAAGCTGAAGGCAGATACATTTGTACGTCTTACGTGATCAAGGTGCAGGCTTTGGTGGAGATGTTGAAGGGCCTGTATCCAAATTATAACTACCCTAAAAGAATCATTGGAGCAGTTGAAGACGTGAAGATGAGTTCAGAGAAATTGCAGAAACTTGGTTGGAAGTATAGGCCATTAGAGGAGACTATTGTTGATGCTGTCAACAACTATATGAAGCAAGTGGTATCCTCTGCAAATAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
Epimerase

Protein Analysis

326

Amino Acids

36.1

Weight (kDa)

5.68

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 3 - 139 1.2e-10 NmrA-like family
KR PF08659 6 - 135 3.1e-07 KR domain
Epimerase PF01370 7 - 248 2.1e-30 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 7 - 131 3.4e-07 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 9 - 242 5.8e-21 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 9 - 266 4.3e-16 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 11 - 192 2.3e-16 NAD(P)H-binding
NAD_binding_4 PF07993 77 - 207 5.3e-08 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 822
Acc36I ACCTGC 1 cut(s) 313
AccB1I GGYRCC 2 cut(s) 32, 98
AciI CCGC 1 cut(s) 102
AcsI RAATTY 2 cut(s) 163, 293
AcuI CTGAAG 3 cut(s) 540, 726, 762
AfaI GTAC 2 cut(s) 330, 760
AfiI CCNNNNNNNGG 1 cut(s) 222
AgsI TTSAA 5 cut(s) 139, 178, 200, 802, 854
AjiI CACGTC 1 cut(s) 859
AjnI CCWGG 1 cut(s) 112
AloI GAACNNNNNNTCC 2 cut(s) 75, 107
AluBI AGCT 6 cut(s) 67, 470, 524, 704, 710, 740
AluI AGCT 6 cut(s) 67, 470, 524, 704, 710, 740
Alw21I GWGCWC 1 cut(s) 676
Alw26I GTCTC 2 cut(s) 102, 911
AlwNI CAGNNNCTG 2 cut(s) 518, 740
AoxI GGCC 3 cut(s) 410, 805, 905
ApeKI GCWGC 2 cut(s) 67, 595
ApoI RAATTY 2 cut(s) 163, 293
AspS9I GGNCC 3 cut(s) 411, 587, 805
AsuHPI GGTGA 1 cut(s) 128
AvaII GGWCC 1 cut(s) 587
BaeGI GKGCMC 1 cut(s) 320
BaeI ACNNNNGTAYC 2 cut(s) 742, 775
BanI GGYRCC 2 cut(s) 32, 98
BbsI GAAGAC 1 cut(s) 861
Bbv12I GWGCWC 1 cut(s) 676
BbvI GCAGC 2 cut(s) 54, 582
BccI CCATC 3 cut(s) 268, 580, 641
BciT130I CCWGG 1 cut(s) 114
BciVI GTATCC 3 cut(s) 77, 822, 970
BclI TGATCA 1 cut(s) 771
BcoDI GTCTC 2 cut(s) 102, 911
BfaI CTAG 2 cut(s) 105, 332
BfuAI ACCTGC 1 cut(s) 313
BfuI GTATCC 3 cut(s) 77, 822, 970
BisI GCNGC 2 cut(s) 68, 596
BlsI GCNGC 2 cut(s) 69, 597
Bme1390I CCNGG 1 cut(s) 114
Bme18I GGWCC 1 cut(s) 587
BmgBI CACGTC 1 cut(s) 859
BmgT120I GGNCC 3 cut(s) 411, 587, 805
BmiI GGNNCC 2 cut(s) 34, 100
BmrFI CCNGG 1 cut(s) 114
BmrI ACTGGG 1 cut(s) 472
BmsI GCATC 3 cut(s) 59, 325, 919
BmuI ACTGGG 1 cut(s) 472
BpiI GAAGAC 1 cut(s) 861
BpuEI CTTGAG 1 cut(s) 63
BsaAI YACGTR 1 cut(s) 769
BsaI GGTCTC 1 cut(s) 102
BsaJI CCNNGG 3 cut(s) 112, 414, 426
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse1I ACTGG 4 cut(s) 34, 150, 478, 657
Bse3DI GCAATG 1 cut(s) 623
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 3 cut(s) 112, 414, 426
BseGI GGATG 2 cut(s) 436, 572
BseLI CCNNNNNNNGG 1 cut(s) 222
BseMI GCAATG 1 cut(s) 623
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 4 cut(s) 34, 150, 478, 657
BseRI GAGGAG 1 cut(s) 929
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 2 cut(s) 54, 582
BsgI GTGCAG 1 cut(s) 800
BshFI GGCC 3 cut(s) 412, 807, 907
BshNI GGYRCC 2 cut(s) 32, 98
BsiHKAI GWGCWC 1 cut(s) 676
BsiSI CCGG 1 cut(s) 36
BslI CCNNNNNNNGG 1 cut(s) 222
BsmAI GTCTC 2 cut(s) 102, 911
BsmI GAATGC 1 cut(s) 133
BsnI GGCC 3 cut(s) 412, 807, 907
Bso31I GGTCTC 1 cut(s) 102
Bsp1286I GDGCHC 2 cut(s) 320, 676
Bsp143I GATC 1 cut(s) 771
Bsp19I CCATGG 1 cut(s) 426
BspACI CCGC 1 cut(s) 102
BspANI GGCC 3 cut(s) 412, 807, 907
BspCNI CTCAG 1 cut(s) 151
BspLI GGNNCC 2 cut(s) 34, 100
BspMI ACCTGC 1 cut(s) 313
BspQI GCTCTTC 1 cut(s) 723
BspT107I GGYRCC 2 cut(s) 32, 98
BspTNI GGTCTC 1 cut(s) 102
BsrDI GCAATG 1 cut(s) 623
BsrI ACTGG 4 cut(s) 34, 150, 478, 657
BssECI CCNNGG 3 cut(s) 112, 414, 426
BssMI GATC 1 cut(s) 771
BssT1I CCWWGG 2 cut(s) 414, 426
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 4 cut(s) 19, 174, 565, 671
Bst6I CTCTTC 2 cut(s) 642, 723
BstBAI YACGTR 1 cut(s) 769
BstC8I GCNNGC 3 cut(s) 250, 616, 783
BstDEI CTNAG 2 cut(s) 159, 471
BstDSI CCRYGG 1 cut(s) 426
BstF5I GGATG 2 cut(s) 436, 572
BstKTI GATC 1 cut(s) 774
BstMAI GTCTC 2 cut(s) 102, 911
BstMBI GATC 1 cut(s) 771
BstNI CCWGG 1 cut(s) 114
BstNSI RCATGY 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 112
BstSLI GKGCMC 1 cut(s) 320
BstV1I GCAGC 2 cut(s) 54, 582
BstV2I GAAGAC 1 cut(s) 861
BstXI CCANNNNNNTGG 1 cut(s) 508
BsuI GTATCC 3 cut(s) 77, 822, 970
BsuRI GGCC 3 cut(s) 412, 807, 907
BtgI CCRYGG 1 cut(s) 426
BtrI CACGTC 1 cut(s) 859
BtsCI GGATG 2 cut(s) 436, 572
BtsIMutI CAGTG 1 cut(s) 24
BveI ACCTGC 1 cut(s) 313
Cac8I GCNNGC 3 cut(s) 250, 616, 783
CaiI CAGNNNCTG 2 cut(s) 518, 740
Cfr13I GGNCC 3 cut(s) 411, 587, 805
Csp6I GTAC 2 cut(s) 329, 759
CviAII CATG 4 cut(s) 95, 242, 339, 427
CviQI GTAC 2 cut(s) 329, 759
DdeI CTNAG 2 cut(s) 159, 471
DpnI GATC 1 cut(s) 773
DpnII GATC 1 cut(s) 771
Eam1104I CTCTTC 2 cut(s) 642, 723
EarI CTCTTC 2 cut(s) 642, 723
Eco130I CCWWGG 2 cut(s) 414, 426
Eco31I GGTCTC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 587
Eco57I CTGAAG 3 cut(s) 540, 726, 762
EcoO109I RGGNCCY 1 cut(s) 805
EcoRII CCWGG 1 cut(s) 112
EcoT14I CCWWGG 2 cut(s) 414, 426
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 2 cut(s) 414, 426
FaeI CATG 4 cut(s) 98, 245, 342, 430
FalI AAGNNNNNCTT 4 cut(s) 170, 202, 699, 731
FatI CATG 4 cut(s) 94, 241, 338, 426
FauNDI CATATG 1 cut(s) 724
FbaI TGATCA 1 cut(s) 771
Fnu4HI GCNGC 2 cut(s) 68, 596
FokI GGATG 2 cut(s) 443, 559
Fsp4HI GCNGC 2 cut(s) 68, 596
FspBI CTAG 2 cut(s) 105, 332
GluI GCNGC 2 cut(s) 68, 596
HaeIII GGCC 3 cut(s) 412, 807, 907
HapII CCGG 1 cut(s) 36
Hin1II CATG 4 cut(s) 98, 245, 342, 430
HincII GTYRAC 1 cut(s) 937
HindII GTYRAC 1 cut(s) 937
HindIII AAGCTT 1 cut(s) 522
HinfI GANTC 3 cut(s) 555, 656, 837
HpaII CCGG 1 cut(s) 36
HphI GGTGA 1 cut(s) 128
Hpy166II GTNNAC 1 cut(s) 937
Hpy188I TCNGA 4 cut(s) 160, 265, 448, 874
Hpy8I GTNNAC 1 cut(s) 937
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 3 cut(s) 194, 737, 796
HpyCH4III ACNGT 4 cut(s) 19, 174, 565, 671
HpyCH4IV ACGT 4 cut(s) 195, 761, 768, 858
HpyCH4V TGCA 8 cut(s) 169, 338, 462, 536, 598, 781, 883, 968
HpyF3I CTNAG 2 cut(s) 159, 471
HpySE526I ACGT 4 cut(s) 195, 761, 768, 858
Hsp92II CATG 4 cut(s) 98, 245, 342, 430
Ksp22I TGATCA 1 cut(s) 771
Kzo9I GATC 1 cut(s) 771
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 1 cut(s) 845
Lsp1109I GCAGC 2 cut(s) 54, 582
LweI GCATC 3 cut(s) 59, 325, 919
MaeI CTAG 2 cut(s) 105, 332
MaeII ACGT 4 cut(s) 195, 761, 768, 858
MaeIII GTNAC 1 cut(s) 25
MalI GATC 1 cut(s) 773
MboI GATC 1 cut(s) 771
MboII GAAGA 7 cut(s) 136, 151, 446, 629, 740, 866, 874
MhlI GDGCHC 2 cut(s) 320, 676
MluCI AATT 5 cut(s) 163, 293, 548, 817, 878
MmeI TCCRAC 1 cut(s) 875
MnlI CCTC 4 cut(s) 32, 354, 907, 973
Mph1103I ATGCAT 1 cut(s) 340
MseI TTAA 1 cut(s) 608
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 1 cut(s) 114
Mva1269I GAATGC 1 cut(s) 133
MvaI CCWGG 1 cut(s) 114
NcoI CCATGG 1 cut(s) 426
NdeI CATATG 1 cut(s) 724
NdeII GATC 1 cut(s) 771
NlaIII CATG 4 cut(s) 98, 245, 342, 430
NlaIV GGNNCC 2 cut(s) 34, 100
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 723
PctI GAATGC 1 cut(s) 133
PfeI GAWTC 3 cut(s) 555, 656, 837
PkrI GCNGC 2 cut(s) 69, 597
Ppu21I YACGTR 1 cut(s) 769
PsiI TTATAA 1 cut(s) 822
Psp6I CCWGG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 2 cut(s) 34, 100
PspPI GGNCC 3 cut(s) 411, 587, 805
PstNI CAGNNNCTG 2 cut(s) 518, 740
RsaI GTAC 2 cut(s) 330, 760
RsaNI GTAC 2 cut(s) 329, 759
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 1 cut(s) 608
SatI GCNGC 2 cut(s) 68, 596
Sau3AI GATC 1 cut(s) 771
Sau96I GGNCC 3 cut(s) 411, 587, 805
ScrFI CCNGG 1 cut(s) 114
SduI GDGCHC 2 cut(s) 320, 676
SfaNI GCATC 3 cut(s) 59, 325, 919
SinI GGWCC 1 cut(s) 587
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
Sse9I AATT 5 cut(s) 163, 293, 548, 817, 878
SsiI CCGC 1 cut(s) 102
SspMI CTAG 2 cut(s) 105, 332
StyD4I CCNGG 1 cut(s) 112
StyI CCWWGG 2 cut(s) 414, 426
TaaI ACNGT 4 cut(s) 19, 174, 565, 671
TaiI ACGT 4 cut(s) 198, 764, 771, 861
TaqI TCGA 2 cut(s) 192, 300
TasI AATT 5 cut(s) 163, 293, 548, 817, 878
TatI WGTACW 1 cut(s) 328
TfiI GAWTC 3 cut(s) 555, 656, 837
Tru1I TTAA 1 cut(s) 608
Tru9I TTAA 1 cut(s) 608
TscAI CASTG 1 cut(s) 24
TseI GCWGC 2 cut(s) 67, 595
TspDTI ATGAA 5 cut(s) 83, 366, 447, 741, 962
TspGWI ACGGA 1 cut(s) 337
TspRI CASTG 1 cut(s) 24
VpaK11BI GGWCC 1 cut(s) 587
XapI RAATTY 2 cut(s) 163, 293
XceI RCATGY 1 cut(s) 342
XspI CTAG 2 cut(s) 105, 332
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.