pycom06g00360

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001551
Physical Location & Seq
Forward (+)
358434 .. 359111
678 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g00360.1

Sequence Viewer

Length: 678 bp
ATGAATGTGTTGCAGGAGTTTCTTGAACCAGCAATAAAGGGAACGCTGAATGTACTCAAGGCTTGCAAAGAAGCCAAAGTGAAGAGAGTTGTAGTTGTGTCGTCTTTAGCCGCTGTTGTTATGAACCTGGAGTGGCCTAAGGATCAAGTCAAGGACGAGACTTGTTGGTCCGTTCCCGAATATATGAAAACAACCAAGAAATGGTACTATCTTTCGAAAACGGAAGCAGAACGCGAGGCTCTGGAGTTTGGAAAAAGGAATGGACTTGAGGTTGTGACTGTTTGTCCTTCTCTCATTTTGGGGCCAATTTTGCAATCCACTCGCAAATGCAGTAGCTTTCTCATTGTTATGACTATAATAGGTATCTCTGTGCATATTCATATATTAAGAGTACAAGGTACAGGACATGTTTCTTGTTTGTGCAATATTAATGGGCTAGTGTGTGTGCACACGCGCACGACTCTGTACGAATGTGTAGGTGATCTTGAATCCTTGCCATGCAATTACTGGATGTTCGTGGATGTTCGTGATTTGGCTGAAGCGCTGCTTCTTGCATACAAGAAGTCTGAGGCTGCAGGTGAAAGATACATATGCCATTCACACTCAATTGGCATACGAGATGTGGTGGAGAAATACTTAAGGCCTACATATCCCGATTACAAGTACCCCAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

226

Amino Acids

25.52

Weight (kDa)

8.61

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_4 PF07993 4 - 106 6.4e-08 Male sterility protein
Epimerase PF01370 6 - 107 5.4e-08 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 8 - 105 7.2e-08 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 566
AasI GACNNNNNNGTC 1 cut(s) 166
Acc36I ACCTGC 1 cut(s) 566
AccB7I CCANNNNNTGG 1 cut(s) 201
AccII CGCG 2 cut(s) 234, 454
AciI CCGC 1 cut(s) 111
AclWI GGATC 1 cut(s) 150
AcuI CTGAAG 1 cut(s) 558
AfaI GTAC 6 cut(s) 54, 206, 393, 400, 467, 665
AfeI AGCGCT 1 cut(s) 543
AfiI CCNNNNNNNGG 1 cut(s) 201
AflII CTTAAG 1 cut(s) 637
AflIII ACRYGT 1 cut(s) 406
AgsI TTSAA 2 cut(s) 26, 488
AhdI GACNNNNNGTC 1 cut(s) 282
AjnI CCWGG 1 cut(s) 126
AluBI AGCT 1 cut(s) 336
AluI AGCT 1 cut(s) 336
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 1 cut(s) 152
Alw44I GTGCAC 1 cut(s) 446
AlwI GGATC 1 cut(s) 150
Aor51HI AGCGCT 1 cut(s) 543
AoxI GGCC 3 cut(s) 134, 302, 641
ApaLI GTGCAC 1 cut(s) 446
ApeKI GCWGC 2 cut(s) 544, 572
AseI ATTAAT 1 cut(s) 429
AspLEI GCGC 2 cut(s) 456, 544
AspS9I GGNCC 2 cut(s) 168, 302
AsuHPI GGTGA 2 cut(s) 491, 590
AsuII TTCGAA 1 cut(s) 215
AvaII GGWCC 1 cut(s) 168
AxyI CCTNAGG 1 cut(s) 138
BaeGI GKGCMC 1 cut(s) 450
Bbv12I GWGCWC 1 cut(s) 450
BbvI GCAGC 2 cut(s) 531, 559
BcgI CGANNNNNNTGC 2 cut(s) 302, 336
BciT130I CCWGG 1 cut(s) 128
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 1 cut(s) 437
BfmI CTRYAG 1 cut(s) 573
BfoI RGCGCY 1 cut(s) 545
BfrI CTTAAG 1 cut(s) 637
BfuAI ACCTGC 1 cut(s) 566
BisI GCNGC 3 cut(s) 111, 545, 573
BlsI GCNGC 3 cut(s) 112, 546, 574
Bme1390I CCNGG 1 cut(s) 128
Bme18I GGWCC 1 cut(s) 168
BmeRI GACNNNNNGTC 1 cut(s) 282
BmgT120I GGNCC 2 cut(s) 168, 302
BmiI GGNNCC 1 cut(s) 303
BmrFI CCNGG 1 cut(s) 128
BpmI CTGGAG 2 cut(s) 149, 263
Bpu14I TTCGAA 1 cut(s) 215
BpuEI CTTGAG 2 cut(s) 41, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 201
Bse1I ACTGG 1 cut(s) 512
Bse21I CCTNAGG 1 cut(s) 138
BseBI CCWGG 1 cut(s) 128
BseGI GGATG 2 cut(s) 516, 526
BseLI CCNNNNNNNGG 1 cut(s) 201
BseMII CTCAG 1 cut(s) 558
BseNI ACTGG 1 cut(s) 512
BseSI GKGCMC 1 cut(s) 450
BseXI GCAGC 2 cut(s) 531, 559
Bsh1236I CGCG 2 cut(s) 234, 454
BshFI GGCC 3 cut(s) 136, 304, 643
BsiHKAI GWGCWC 1 cut(s) 450
BslI CCNNNNNNNGG 1 cut(s) 201
BsmAI GTCTC 1 cut(s) 152
BsnI GGCC 3 cut(s) 136, 304, 643
Bsp119I TTCGAA 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 450
Bsp143I GATC 2 cut(s) 142, 481
BspACI CCGC 1 cut(s) 111
BspANI GGCC 3 cut(s) 136, 304, 643
BspCNI CTCAG 1 cut(s) 559
BspFNI CGCG 2 cut(s) 234, 454
BspLI GGNNCC 1 cut(s) 303
BspMAI CTGCAG 1 cut(s) 577
BspMI ACCTGC 1 cut(s) 566
BspPI GGATC 1 cut(s) 150
BspT104I TTCGAA 1 cut(s) 215
BspTI CTTAAG 1 cut(s) 637
BsrI ACTGG 1 cut(s) 512
BssMI GATC 2 cut(s) 142, 481
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 1 cut(s) 77
BstAFI CTTAAG 1 cut(s) 637
BstBI TTCGAA 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 64
BstDEI CTNAG 2 cut(s) 138, 567
BstF5I GGATG 2 cut(s) 516, 526
BstFNI CGCG 2 cut(s) 234, 454
BstH2I RGCGCY 1 cut(s) 545
BstHHI GCGC 2 cut(s) 456, 544
BstKTI GATC 2 cut(s) 145, 484
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 2 cut(s) 142, 481
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstNI CCWGG 1 cut(s) 128
BstNSI RCATGY 1 cut(s) 410
BstSCI CCNGG 1 cut(s) 126
BstSFI CTRYAG 1 cut(s) 573
BstSLI GKGCMC 1 cut(s) 450
BstUI CGCG 2 cut(s) 234, 454
BstV1I GCAGC 2 cut(s) 531, 559
Bsu36I CCTNAGG 1 cut(s) 138
BsuRI GGCC 3 cut(s) 136, 304, 643
BtsCI GGATG 2 cut(s) 516, 526
BveI ACCTGC 1 cut(s) 566
Cac8I GCNNGC 1 cut(s) 64
CfoI GCGC 2 cut(s) 456, 544
Cfr13I GGNCC 2 cut(s) 168, 302
Csp6I GTAC 6 cut(s) 53, 205, 392, 399, 466, 664
CviAII CATG 2 cut(s) 407, 498
CviQI GTAC 6 cut(s) 53, 205, 392, 399, 466, 664
DdeI CTNAG 2 cut(s) 138, 567
DpnI GATC 2 cut(s) 144, 483
DpnII GATC 2 cut(s) 142, 481
DrdI GACNNNNNNGTC 1 cut(s) 166
DriI GACNNNNNGTC 1 cut(s) 282
DseDI GACNNNNNNGTC 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 77
Eam1105I GACNNNNNGTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 77
Eco147I AGGCCT 1 cut(s) 643
Eco47I GGWCC 1 cut(s) 168
Eco47III AGCGCT 1 cut(s) 543
Eco57I CTGAAG 1 cut(s) 558
Eco81I CCTNAGG 1 cut(s) 138
EcoRII CCWGG 1 cut(s) 126
FaeI CATG 2 cut(s) 410, 501
FalI AAGNNNNNCTT 2 cut(s) 531, 563
FatI CATG 2 cut(s) 406, 497
FauNDI CATATG 1 cut(s) 590
Fnu4HI GCNGC 3 cut(s) 111, 545, 573
FokI GGATG 2 cut(s) 523, 533
Fsp4HI GCNGC 3 cut(s) 111, 545, 573
FspBI CTAG 1 cut(s) 437
GlaI GCGC 2 cut(s) 455, 543
GluI GCNGC 3 cut(s) 111, 545, 573
GsuI CTGGAG 2 cut(s) 149, 263
HaeII RGCGCY 1 cut(s) 545
HaeIII GGCC 3 cut(s) 136, 304, 643
HhaI GCGC 2 cut(s) 456, 544
Hin1II CATG 2 cut(s) 410, 501
Hin6I GCGC 2 cut(s) 454, 542
HinP1I GCGC 2 cut(s) 454, 542
HinfI GANTC 2 cut(s) 460, 488
HphI GGTGA 2 cut(s) 491, 590
Hpy166II GTNNAC 1 cut(s) 448
Hpy188I TCNGA 1 cut(s) 568
Hpy188III TCNNGA 6 cut(s) 23, 176, 242, 485, 527, 653
Hpy8I GTNNAC 1 cut(s) 448
HpyAV CCTTC 1 cut(s) 297
HpyCH4III ACNGT 1 cut(s) 280
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
HpyF3I CTNAG 2 cut(s) 138, 567
Hsp92II CATG 2 cut(s) 410, 501
HspAI GCGC 2 cut(s) 454, 542
Kzo9I GATC 2 cut(s) 142, 481
LpnPI CCDG 7 cut(s) 42, 113, 140, 227, 387, 493, 561
Lsp1109I GCAGC 2 cut(s) 531, 559
MaeI CTAG 1 cut(s) 437
MaeIII GTNAC 1 cut(s) 274
MalI GATC 2 cut(s) 144, 483
MboI GATC 2 cut(s) 142, 481
MboII GAAGA 1 cut(s) 94
MfeI CAATTG 1 cut(s) 606
MhlI GDGCHC 1 cut(s) 450
MluCI AATT 3 cut(s) 306, 502, 606
MlyI GAGTC 1 cut(s) 454
MnlI CCTC 3 cut(s) 229, 262, 562
MseI TTAA 3 cut(s) 386, 429, 638
MslI CAYNNNNRTG 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 113
MspCI CTTAAG 1 cut(s) 637
MspR9I CCNGG 1 cut(s) 128
MunI CAATTG 1 cut(s) 606
MvaI CCWGG 1 cut(s) 128
MvnI CGCG 2 cut(s) 234, 454
MwoI GCNNNNNNNGC 1 cut(s) 310
NdeI CATATG 1 cut(s) 590
NdeII GATC 2 cut(s) 142, 481
NlaIII CATG 2 cut(s) 410, 501
NlaIV GGNNCC 1 cut(s) 303
NmuCI GTSAC 1 cut(s) 274
NspI RCATGY 1 cut(s) 410
NspV TTCGAA 1 cut(s) 215
PaqCI CACCTGC 1 cut(s) 566
PceI AGGCCT 1 cut(s) 643
PciI ACATGT 1 cut(s) 406
PfeI GAWTC 1 cut(s) 488
PflMI CCANNNNNTGG 1 cut(s) 201
PkrI GCNGC 3 cut(s) 112, 546, 574
PleI GAGTC 1 cut(s) 454
PpsI GAGTC 1 cut(s) 454
PscI ACATGT 1 cut(s) 406
PshBI ATTAAT 1 cut(s) 429
Psp6I CCWGG 1 cut(s) 126
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 303
PspPI GGNCC 2 cut(s) 168, 302
PstI CTGCAG 1 cut(s) 577
RsaI GTAC 6 cut(s) 54, 206, 393, 400, 467, 665
RsaNI GTAC 6 cut(s) 53, 205, 392, 399, 466, 664
RseI CAYNNNNRTG 1 cut(s) 347
SaqAI TTAA 3 cut(s) 386, 429, 638
SatI GCNGC 3 cut(s) 111, 545, 573
Sau3AI GATC 2 cut(s) 142, 481
Sau96I GGNCC 2 cut(s) 168, 302
SchI GAGTC 1 cut(s) 454
ScrFI CCNGG 1 cut(s) 128
SduI GDGCHC 1 cut(s) 450
SetI ASST 7 cut(s) 129, 273, 338, 364, 400, 481, 580
SfcI CTRYAG 1 cut(s) 573
SfuI TTCGAA 1 cut(s) 215
SinI GGWCC 1 cut(s) 168
SmiMI CAYNNNNRTG 1 cut(s) 347
SmlI CTYRAG 3 cut(s) 56, 266, 637
SmoI CTYRAG 3 cut(s) 56, 266, 637
Sse9I AATT 3 cut(s) 306, 502, 606
SseBI AGGCCT 1 cut(s) 643
SsiI CCGC 1 cut(s) 111
SspI AATATT 1 cut(s) 427
SspMI CTAG 1 cut(s) 437
StuI AGGCCT 1 cut(s) 643
StyD4I CCNGG 1 cut(s) 126
TaaI ACNGT 1 cut(s) 280
TaqI TCGA 1 cut(s) 215
TasI AATT 3 cut(s) 306, 502, 606
TatI WGTACW 2 cut(s) 52, 391
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 1 cut(s) 488
Tru1I TTAA 3 cut(s) 386, 429, 638
Tru9I TTAA 3 cut(s) 386, 429, 638
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 2 cut(s) 544, 572
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 4 cut(s) 17, 137, 200, 368
TspGWI ACGGA 2 cut(s) 160, 236
Van91I CCANNNNNTGG 1 cut(s) 201
Vha464I CTTAAG 1 cut(s) 637
VneI GTGCAC 1 cut(s) 446
VpaK11BI GGWCC 1 cut(s) 168
VspI ATTAAT 1 cut(s) 429
XceI RCATGY 1 cut(s) 410
XcmI CCANNNNNNNNNTGG 1 cut(s) 504
XspI CTAG 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.