Rroxscaffold_3G00242850

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001551
Physical Location & Seq
Forward (+)
35354190 .. 35355464
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00242850.1

Sequence Viewer

Length: 876 bp
ATGACAAATGTTATGCAATCTGCAGGGGATAGCAAGTATGATCACTTGAACAACCTGGAGAGATCACCAGAGAACCTGAAGCTGTTCAAGGCAGATTTGCTGGATTATGAGTCACTCAGATTGGCGATTTCAGGATGCAGTGGAGTGTTCCACGTGGCCAGTCCTGTACCTTTTCCAACTGCCATAACAGATCCCGAAGCAGAAGTACTTAAACCTGCTGTGGGGGGAACACTTAATGTATTGAAAGCTTGCGTAGAGGCCAAAGTGAAGAGAGTTGTTGTTGTGTCGTCTATAGCTGCTGTTGTGAAGAACCCGAACTGGCCCAAGGATCAAGTGAAAGACGAGACATGTTGGTCTGATCCGGAATATGTTAAGTCCACTACGAATTGGTATTATCTTTCCAAAACACAAGCTGAAGCTGCAGCCTTGGAGTTCGGAAGAAGCAGTGGACTTGATGTTGTAACTGTTTGTCCTTCTCTTATCATGGGGCCACTGTTGCAATCCACTCTGAATACTAGTAGCTGGCTCCTCCCTATGGGATTCAAAGGCGGGTTTGAGAAGGTGGGCTACAATTACTGGACGGTGGTGGATGCTCGTGATTTAGCTGATGCACTGCTTCTTGTATACAAGAAATCTGAGGCTGGAGAAAGATACATCTGCACAGCACATGCAATTGGGGTAGAAGAAGTAGTGGAGAAGTACTTGAAGATTTCACATCCCAACTACAACTATTCTCCCGAGCTTACGCATACGAAGGAAGAAGAGGAGAAGCTGAGTTCGGAAAAATTGGAGAGGTTGGGTTGGAGTTACCGGCCATTGGGGGAAACTCTTATTGATTCCATTGAAAGCTACCAGAAGATTGGAGCCATGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

291

Amino Acids

32.27

Weight (kDa)

5.21

Isoelectric Point (pI)

35.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDP_Man_Dehyd PF16363 12 - 237 2.1e-06 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 15 - 159 1.2e-10 NAD(P)H-binding
Epimerase PF01370 22 - 217 3e-16 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 24 - 171 2.5e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 37 - 187 1.8e-09 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 352
Acc36I ACCTGC 1 cut(s) 223
AccI GTMKAC 1 cut(s) 624
AccIII TCCGGA 1 cut(s) 361
AciI CCGC 1 cut(s) 549
AclWI GGATC 3 cut(s) 185, 336, 353
AcoI YGGCCR 2 cut(s) 156, 812
AcuI CTGAAG 2 cut(s) 98, 435
AcvI CACGTG 1 cut(s) 154
AfaI GTAC 3 cut(s) 168, 207, 701
AfiI CCNNNNNNNGG 3 cut(s) 221, 535, 817
AflIII ACRYGT 1 cut(s) 347
AgsI TTSAA 6 cut(s) 49, 88, 244, 544, 706, 845
AhlI ACTAGT 1 cut(s) 515
AjnI CCWGG 1 cut(s) 54
Alw26I GTCTC 1 cut(s) 338
AlwI GGATC 3 cut(s) 185, 336, 353
Ama87I CYCGRG 1 cut(s) 737
Aor13HI TCCGGA 1 cut(s) 361
AoxI GGCC 5 cut(s) 156, 258, 320, 488, 812
ApeKI GCWGC 3 cut(s) 296, 419, 422
Asp700I GAANNNNTTC 1 cut(s) 83
AspS9I GGNCC 2 cut(s) 321, 488
AsuHPI GGTGA 1 cut(s) 57
AvaI CYCGRG 1 cut(s) 737
BalI TGGCCA 1 cut(s) 158
BarI GAAGNNNNNNTAC 4 cut(s) 189, 221, 551, 583
BauI CACGAG 1 cut(s) 594
BbrPI CACGTG 1 cut(s) 154
BbvI GCAGC 3 cut(s) 283, 406, 434
BciT130I CCWGG 1 cut(s) 56
BclI TGATCA 1 cut(s) 40
BcoDI GTCTC 1 cut(s) 338
BcuI ACTAGT 1 cut(s) 515
BfaI CTAG 1 cut(s) 516
BfmI CTRYAG 3 cut(s) 21, 291, 420
BfuAI ACCTGC 1 cut(s) 223
BisI GCNGC 3 cut(s) 297, 420, 423
BlsI GCNGC 3 cut(s) 298, 421, 424
BmcAI AGTACT 2 cut(s) 207, 701
Bme1390I CCNGG 1 cut(s) 56
BmeT110I CYCGRG 1 cut(s) 737
BmgT120I GGNCC 2 cut(s) 321, 488
BmiI GGNNCC 3 cut(s) 489, 527, 865
BmrFI CCNGG 1 cut(s) 56
BmsI GCATC 3 cut(s) 125, 580, 598
BpmI CTGGAG 2 cut(s) 77, 663
BsaAI YACGTR 1 cut(s) 154
BsaJI CCNNGG 3 cut(s) 324, 426, 867
BsaWI WCCGGW 1 cut(s) 361
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 535, 817
Bse118I RCCGGY 1 cut(s) 810
Bse1I ACTGG 3 cut(s) 159, 323, 581
BseAI TCCGGA 1 cut(s) 361
BseBI CCWGG 1 cut(s) 56
BseDI CCNNGG 3 cut(s) 324, 426, 867
BseGI GGATG 3 cut(s) 140, 595, 715
BseLI CCNNNNNNNGG 3 cut(s) 221, 535, 817
BseMII CTCAG 3 cut(s) 130, 627, 764
BseNI ACTGG 3 cut(s) 159, 323, 581
BseRI GAGGAG 2 cut(s) 518, 779
BseXI GCAGC 3 cut(s) 283, 406, 434
BsgI GTGCAG 1 cut(s) 643
BshFI GGCC 5 cut(s) 158, 260, 322, 490, 814
BsiHKCI CYCGRG 1 cut(s) 737
BsiSI CCGG 2 cut(s) 362, 811
BslI CCNNNNNNNGG 3 cut(s) 221, 535, 817
BsmAI GTCTC 1 cut(s) 338
BsnI GGCC 5 cut(s) 158, 260, 322, 490, 814
BsoBI CYCGRG 1 cut(s) 737
Bsp13I TCCGGA 1 cut(s) 361
Bsp143I GATC 5 cut(s) 40, 62, 190, 328, 358
Bsp19I CCATGG 1 cut(s) 867
BspACI CCGC 1 cut(s) 549
BspANI GGCC 5 cut(s) 158, 260, 322, 490, 814
BspCNI CTCAG 3 cut(s) 129, 628, 765
BspEI TCCGGA 1 cut(s) 361
BspLI GGNNCC 3 cut(s) 489, 527, 865
BspMAI CTGCAG 2 cut(s) 25, 424
BspMI ACCTGC 1 cut(s) 223
BspPI GGATC 3 cut(s) 185, 336, 353
BsrFI RCCGGY 1 cut(s) 810
BsrI ACTGG 3 cut(s) 159, 323, 581
BssAI RCCGGY 1 cut(s) 810
BssECI CCNNGG 3 cut(s) 324, 426, 867
BssMI GATC 5 cut(s) 40, 62, 190, 328, 358
BssNAI GTATAC 1 cut(s) 625
BssSI CACGAG 1 cut(s) 594
BssT1I CCWWGG 3 cut(s) 324, 426, 867
Bst1107I GTATAC 1 cut(s) 625
Bst2BI CACGAG 1 cut(s) 594
Bst2UI CCWGG 1 cut(s) 56
Bst4CI ACNGT 3 cut(s) 466, 495, 583
Bst6I CTCTTC 2 cut(s) 263, 756
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 2 cut(s) 250, 524
BstDEI CTNAG 3 cut(s) 116, 636, 773
BstDSI CCRYGG 1 cut(s) 867
BstF5I GGATG 3 cut(s) 140, 595, 715
BstKTI GATC 5 cut(s) 43, 65, 193, 331, 361
BstMAI GTCTC 1 cut(s) 338
BstMBI GATC 5 cut(s) 40, 62, 190, 328, 358
BstMWI GCNNNNNNNGC 2 cut(s) 419, 496
BstNI CCWGG 1 cut(s) 56
BstNSI RCATGY 2 cut(s) 351, 671
BstSCI CCNGG 1 cut(s) 54
BstSFI CTRYAG 3 cut(s) 21, 291, 420
BstV1I GCAGC 3 cut(s) 283, 406, 434
BstX2I RGATCY 1 cut(s) 190
BstXI CCANNNNNNTGG 1 cut(s) 860
BstYI RGATCY 1 cut(s) 190
BstZ17I GTATAC 1 cut(s) 625
BsuRI GGCC 5 cut(s) 158, 260, 322, 490, 814
BtgI CCRYGG 1 cut(s) 867
BtsCI GGATG 3 cut(s) 140, 595, 715
BtsI GCAGTG 3 cut(s) 145, 451, 611
BtsIMutI CAGTG 4 cut(s) 145, 451, 491, 611
BveI ACCTGC 1 cut(s) 223
Cac8I GCNNGC 2 cut(s) 250, 524
Cfr10I RCCGGY 1 cut(s) 810
Cfr13I GGNCC 2 cut(s) 321, 488
Csp6I GTAC 3 cut(s) 167, 206, 700
CviAII CATG 4 cut(s) 348, 484, 668, 868
CviQI GTAC 3 cut(s) 167, 206, 700
DdeI CTNAG 3 cut(s) 116, 636, 773
DpnI GATC 5 cut(s) 42, 64, 192, 330, 360
DpnII GATC 5 cut(s) 40, 62, 190, 328, 358
DrdI GACNNNNNNGTC 1 cut(s) 352
DseDI GACNNNNNNGTC 1 cut(s) 352
EaeI YGGCCR 2 cut(s) 156, 812
Eam1104I CTCTTC 2 cut(s) 263, 756
EarI CTCTTC 2 cut(s) 263, 756
Eco130I CCWWGG 3 cut(s) 324, 426, 867
Eco57I CTGAAG 2 cut(s) 98, 435
Eco72I CACGTG 1 cut(s) 154
Eco88I CYCGRG 1 cut(s) 737
EcoRII CCWGG 1 cut(s) 54
EcoT14I CCWWGG 3 cut(s) 324, 426, 867
ErhI CCWWGG 3 cut(s) 324, 426, 867
FaeI CATG 4 cut(s) 351, 487, 671, 871
FatI CATG 4 cut(s) 347, 483, 667, 867
FauI CCCGC 1 cut(s) 542
FbaI TGATCA 1 cut(s) 40
FblI GTMKAC 1 cut(s) 624
Fnu4HI GCNGC 3 cut(s) 297, 420, 423
FokI GGATG 3 cut(s) 147, 602, 702
Fsp4HI GCNGC 3 cut(s) 297, 420, 423
FspBI CTAG 1 cut(s) 516
GluI GCNGC 3 cut(s) 297, 420, 423
GsuI CTGGAG 2 cut(s) 77, 663
HaeIII GGCC 5 cut(s) 158, 260, 322, 490, 814
HapII CCGG 2 cut(s) 362, 811
Hin1II CATG 4 cut(s) 351, 487, 671, 871
HindIII AAGCTT 1 cut(s) 246
HinfI GANTC 3 cut(s) 110, 540, 836
HpaII CCGG 2 cut(s) 362, 811
HphI GGTGA 1 cut(s) 57
Hpy166II GTNNAC 3 cut(s) 378, 449, 625
Hpy188I TCNGA 6 cut(s) 119, 358, 437, 510, 637, 781
Hpy188III TCNNGA 5 cut(s) 132, 194, 362, 596, 737
Hpy8I GTNNAC 3 cut(s) 378, 449, 625
HpyAV CCTTC 3 cut(s) 483, 553, 748
HpyCH4III ACNGT 3 cut(s) 466, 495, 583
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 8 cut(s) 16, 23, 138, 422, 499, 611, 660, 671
HpyF10VI GCNNNNNNNGC 2 cut(s) 419, 496
HpyF3I CTNAG 3 cut(s) 116, 636, 773
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 4 cut(s) 351, 487, 671, 871
Kpn2I TCCGGA 1 cut(s) 361
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 5 cut(s) 40, 62, 190, 328, 358
LmnI GCTCC 2 cut(s) 531, 863
Lsp1109I GCAGC 3 cut(s) 283, 406, 434
LweI GCATC 3 cut(s) 125, 580, 598
MaeI CTAG 1 cut(s) 516
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 3 cut(s) 111, 460, 806
MalI GATC 5 cut(s) 42, 64, 192, 330, 360
MboI GATC 5 cut(s) 40, 62, 190, 328, 358
MboII GAAGA 8 cut(s) 280, 319, 450, 695, 718, 770, 773, 868
MfeI CAATTG 1 cut(s) 672
MflI RGATCY 1 cut(s) 190
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 4 cut(s) 385, 571, 672, 785
MluNI TGGCCA 1 cut(s) 158
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 2 cut(s) 200, 782
MnlI CCTC 5 cut(s) 250, 539, 631, 757, 786
Mox20I TGGCCA 1 cut(s) 158
MroI TCCGGA 1 cut(s) 361
MroXI GAANNNNTTC 1 cut(s) 83
MscI TGGCCA 1 cut(s) 158
MseI TTAA 3 cut(s) 210, 234, 372
Msp20I TGGCCA 1 cut(s) 158
MspI CCGG 2 cut(s) 362, 811
MspR9I CCNGG 1 cut(s) 56
MunI CAATTG 1 cut(s) 672
MvaI CCWGG 1 cut(s) 56
MwoI GCNNNNNNNGC 2 cut(s) 419, 496
NcoI CCATGG 1 cut(s) 867
NdeII GATC 5 cut(s) 40, 62, 190, 328, 358
NlaIII CATG 4 cut(s) 351, 487, 671, 871
NlaIV GGNNCC 3 cut(s) 489, 527, 865
NmuCI GTSAC 1 cut(s) 111
NspI RCATGY 2 cut(s) 351, 671
PciI ACATGT 1 cut(s) 347
PdmI GAANNNNTTC 1 cut(s) 83
PfeI GAWTC 2 cut(s) 540, 836
PkrI GCNGC 3 cut(s) 298, 421, 424
PleI GAGTC 1 cut(s) 118
PmaCI CACGTG 1 cut(s) 154
PmlI CACGTG 1 cut(s) 154
PpsI GAGTC 1 cut(s) 118
Ppu21I YACGTR 1 cut(s) 154
PscI ACATGT 1 cut(s) 347
Psp6I CCWGG 1 cut(s) 54
PspCI CACGTG 1 cut(s) 154
PspGI CCWGG 1 cut(s) 54
PspN4I GGNNCC 3 cut(s) 489, 527, 865
PspPI GGNCC 2 cut(s) 321, 488
PstI CTGCAG 2 cut(s) 25, 424
PsuI RGATCY 1 cut(s) 190
RsaI GTAC 3 cut(s) 168, 207, 701
RsaNI GTAC 3 cut(s) 167, 206, 700
SaqAI TTAA 3 cut(s) 210, 234, 372
SatI GCNGC 3 cut(s) 297, 420, 423
Sau3AI GATC 5 cut(s) 40, 62, 190, 328, 358
Sau96I GGNCC 2 cut(s) 321, 488
ScaI AGTACT 2 cut(s) 207, 701
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 1 cut(s) 56
SfaNI GCATC 3 cut(s) 125, 580, 598
SfcI CTRYAG 3 cut(s) 21, 291, 420
SpeI ACTAGT 1 cut(s) 515
Sse9I AATT 4 cut(s) 385, 571, 672, 785
SsiI CCGC 1 cut(s) 549
SspMI CTAG 1 cut(s) 516
StyD4I CCNGG 1 cut(s) 54
StyI CCWWGG 3 cut(s) 324, 426, 867
TaaI ACNGT 3 cut(s) 466, 495, 583
TaiI ACGT 1 cut(s) 156
TasI AATT 4 cut(s) 385, 571, 672, 785
TatI WGTACW 2 cut(s) 205, 699
TfiI GAWTC 2 cut(s) 540, 836
Tru1I TTAA 3 cut(s) 210, 234, 372
Tru9I TTAA 3 cut(s) 210, 234, 372
TscAI CASTG 4 cut(s) 145, 451, 498, 618
TseFI GTSAC 1 cut(s) 111
TseI GCWGC 3 cut(s) 296, 419, 422
Tsp45I GTSAC 1 cut(s) 111
TspRI CASTG 4 cut(s) 145, 451, 498, 618
XceI RCATGY 2 cut(s) 351, 671
XmiI GTMKAC 1 cut(s) 624
XmnI GAANNNNTTC 1 cut(s) 83
XspI CTAG 1 cut(s) 516
ZrmI AGTACT 2 cut(s) 207, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.