FvH4_6g27190
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000806
Physical Location & Seq
Forward (+)
20887993 .. 20890971
2979 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27190.t1

Sequence Viewer

Length: 978 bp
ATGGGACGGAGAAAGATAGAAATCAAGAAAATCAGTAACAAAAACTACCTCATGACCTCATTCACTAAGCGGCGAAACGGCCTCTTTCACAAAGCCAGTCAATTTTGCAGGTTATCCGGTGCCGAAATCGCGGTCATTGTCTTCTCGCCAGGTGGCCGTGTTTACCTGTTTGGCCACCCTTCGGCAGACTCCGTCATTGATCGGTATGATATACGAACTGAAGATAACAATAATGAAGGCACACGAGAGTTTCCATATACAATTGACGACGAGGAGGATGATGCTCATGATGATGATATGAGCAGCAGCGAGCTTGCAAACGAGGCAGCTGATGGTAAACATGAGCTTGGAAAGGCCGGCAGGTTTTGGTGGGATAATCTGCCTATTGAAGATATGGAACTAAAACAACTTGAGCAATACAAAAGTTGCTTAGAAGTTCTAAGAAACGATCTAGCATATAAATTATATGATAGGAAGAGGAGAGATTCACACACCAAAGATTTTTTGAGTTTGATCGGCTCCAGTAGTAGTGGTATTGCAGATATGGAACTAAAACAACATGAGCAATATAAAGGTTGCTCATCAGAAGTTTTCAGAAACGAGATGGCATATGAATTATATGAGACGAAGAGGAGAGAATCACAAACCAGAGATTTTTTGAGTTTGATCGGAGAGAATCACAAACCACGATGGAACTTCTACCGATCACCCTATAGAGATAGTGATGTGGGAATGGGTCTTCGGTTGAGAACGTTCTTGAGATGGTTAATTCACTCGGGGGCTCGATCTCATCATTGTCAACAATTTTGTTTTTGCGGAAACTGGCTCCTACGTACAAATGAGTTCAAATCCAATGTTGATGGCGCTTATGTACAACAGTTGCCTAAAGGAGGACTTGCATTTGTGGTGTTTGCTACAGTACAACAGGGCCACTTAGCAAAACCATTTGGTCCTTCAAGGCCTTCGTCAGATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04013 ko04022 ko04111 ko04371 ko04921 ko05202 ko05418 map04010 map04011 map04013 map04022 map04111 map04371 map04921 map05202 map05418
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

326

Amino Acids

37.6

Weight (kDa)

7.17

Isoelectric Point (pI)

55.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 1.7e-16 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 99, 351
AccB1I GGYRCC 1 cut(s) 119
AccII CGCG 1 cut(s) 131
AciI CCGC 3 cut(s) 70, 131, 816
AclI AACGTT 1 cut(s) 752
AcoI YGGCCR 2 cut(s) 154, 172
AcuI CTGAAG 1 cut(s) 240
AfaI GTAC 3 cut(s) 835, 873, 921
AfiI CCNNNNNNNGG 2 cut(s) 181, 890
AgsI TTSAA 3 cut(s) 389, 847, 957
AjnI CCWGG 1 cut(s) 148
AluBI AGCT 3 cut(s) 313, 329, 346
AluI AGCT 3 cut(s) 313, 329, 346
Alw26I GTCTC 1 cut(s) 617
Ama87I CYCGRG 1 cut(s) 775
AoxI GGCC 6 cut(s) 79, 154, 172, 354, 928, 959
ApeKI GCWGC 3 cut(s) 303, 306, 326
ArsI GACNNNNNNTTYG 2 cut(s) 117, 149
AspLEI GCGC 1 cut(s) 866
AspS9I GGNCC 2 cut(s) 928, 950
AsuHPI GGTGA 1 cut(s) 699
AvaI CYCGRG 1 cut(s) 775
AvaII GGWCC 1 cut(s) 950
BalI TGGCCA 1 cut(s) 174
BanI GGYRCC 1 cut(s) 119
BanII GRGCYC 1 cut(s) 784
BauI CACGAG 1 cut(s) 243
BbsI GAAGAC 2 cut(s) 133, 731
BbvI GCAGC 3 cut(s) 315, 318, 338
BccI CCATC 5 cut(s) 326, 598, 684, 756, 854
BceAI ACGGC 2 cut(s) 94, 141
BciT130I CCWGG 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 617
BfaI CTAG 1 cut(s) 452
BfmI CTRYAG 2 cut(s) 712, 915
BfoI RGCGCY 1 cut(s) 867
BfuAI ACCTGC 2 cut(s) 99, 351
BglII AGATCT 1 cut(s) 970
BisI GCNGC 4 cut(s) 71, 304, 307, 327
BlsI GCNGC 4 cut(s) 72, 305, 308, 328
Bme1390I CCNGG 1 cut(s) 150
Bme18I GGWCC 1 cut(s) 950
BmeT110I CYCGRG 1 cut(s) 775
BmgT120I GGNCC 2 cut(s) 928, 950
BmiI GGNNCC 3 cut(s) 121, 520, 827
BmrFI CCNGG 1 cut(s) 150
BmsI GCATC 1 cut(s) 271
BpiI GAAGAC 2 cut(s) 133, 731
BpmI CTGGAG 1 cut(s) 505
BpuEI CTTGAG 2 cut(s) 431, 778
BsaAI YACGTR 1 cut(s) 833
BsaBI GATNNNNATC 1 cut(s) 20
BsaWI WCCGGW 1 cut(s) 116
Bsc4I CCNNNNNNNGG 2 cut(s) 181, 890
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 3 cut(s) 96, 522, 827
Bse8I GATNNNNATC 1 cut(s) 20
BseBI CCWGG 1 cut(s) 150
BseGI GGATG 1 cut(s) 283
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 2 cut(s) 181, 890
BseNI ACTGG 3 cut(s) 96, 522, 827
BseRI GAGGAG 3 cut(s) 287, 493, 646
BseXI GCAGC 3 cut(s) 315, 318, 338
Bsh1236I CGCG 1 cut(s) 131
BshFI GGCC 6 cut(s) 81, 156, 174, 356, 930, 961
BshNI GGYRCC 1 cut(s) 119
BsiHKCI CYCGRG 1 cut(s) 775
BsiSI CCGG 2 cut(s) 117, 357
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 2 cut(s) 181, 890
BsmAI GTCTC 1 cut(s) 617
BsmBI CGTCTC 1 cut(s) 617
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 6 cut(s) 81, 156, 174, 356, 930, 961
BsoBI CYCGRG 1 cut(s) 775
Bsp1286I GDGCHC 1 cut(s) 784
Bsp1407I TGTACA 1 cut(s) 871
Bsp143I GATC 7 cut(s) 199, 448, 513, 666, 704, 785, 970
BspACI CCGC 3 cut(s) 70, 131, 816
BspANI GGCC 6 cut(s) 81, 156, 174, 356, 930, 961
BspFNI CGCG 1 cut(s) 131
BspHI TCATGA 2 cut(s) 51, 286
BspLI GGNNCC 3 cut(s) 121, 520, 827
BspMI ACCTGC 2 cut(s) 99, 351
BspT107I GGYRCC 1 cut(s) 119
BsrFI RCCGGY 1 cut(s) 356
BsrGI TGTACA 1 cut(s) 871
BsrI ACTGG 3 cut(s) 96, 522, 827
BssAI RCCGGY 1 cut(s) 356
BssMI GATC 7 cut(s) 199, 448, 513, 666, 704, 785, 970
BssSI CACGAG 1 cut(s) 243
Bst2BI CACGAG 1 cut(s) 243
Bst2UI CCWGG 1 cut(s) 150
Bst4CI ACNGT 2 cut(s) 879, 919
Bst6I CTCTTC 2 cut(s) 470, 623
BstAUI TGTACA 1 cut(s) 871
BstBAI YACGTR 1 cut(s) 833
BstC8I GCNNGC 3 cut(s) 311, 315, 358
BstDEI CTNAG 4 cut(s) 66, 430, 440, 934
BstENI CCTNNNNNAGG 1 cut(s) 888
BstF5I GGATG 1 cut(s) 283
BstFNI CGCG 1 cut(s) 131
BstH2I RGCGCY 1 cut(s) 867
BstHHI GCGC 1 cut(s) 866
BstKTI GATC 7 cut(s) 202, 451, 516, 669, 707, 788, 973
BstMAI GTCTC 1 cut(s) 617
BstMBI GATC 7 cut(s) 199, 448, 513, 666, 704, 785, 970
BstMWI GCNNNNNNNGC 2 cut(s) 128, 323
BstNI CCWGG 1 cut(s) 150
BstSCI CCNGG 1 cut(s) 148
BstSFI CTRYAG 2 cut(s) 712, 915
BstSNI TACGTA 1 cut(s) 833
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 3 cut(s) 315, 318, 338
BstV2I GAAGAC 2 cut(s) 133, 731
BstX2I RGATCY 1 cut(s) 970
BstYI RGATCY 1 cut(s) 970
BsuRI GGCC 6 cut(s) 81, 156, 174, 356, 930, 961
BtsCI GGATG 1 cut(s) 283
BveI ACCTGC 2 cut(s) 99, 351
Cac8I GCNNGC 3 cut(s) 311, 315, 358
CciI TCATGA 2 cut(s) 51, 286
CfoI GCGC 1 cut(s) 866
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 2 cut(s) 928, 950
Csp6I GTAC 3 cut(s) 834, 872, 920
CviAII CATG 4 cut(s) 52, 287, 341, 560
CviQI GTAC 3 cut(s) 834, 872, 920
DdeI CTNAG 4 cut(s) 66, 430, 440, 934
DpnI GATC 7 cut(s) 201, 450, 515, 668, 706, 787, 972
DpnII GATC 7 cut(s) 199, 448, 513, 666, 704, 785, 970
EaeI YGGCCR 2 cut(s) 154, 172
Eam1104I CTCTTC 2 cut(s) 470, 623
EarI CTCTTC 2 cut(s) 470, 623
Eco105I TACGTA 1 cut(s) 833
Eco147I AGGCCT 1 cut(s) 961
Eco24I GRGCYC 1 cut(s) 784
Eco47I GGWCC 1 cut(s) 950
Eco57I CTGAAG 1 cut(s) 240
Eco88I CYCGRG 1 cut(s) 775
EcoNI CCTNNNNNAGG 1 cut(s) 888
EcoRII CCWGG 1 cut(s) 148
EcoT38I GRGCYC 1 cut(s) 784
Esp3I CGTCTC 1 cut(s) 617
FaeI CATG 4 cut(s) 55, 290, 344, 563
FalI AAGNNNNNCTT 2 cut(s) 879, 911
FaqI GGGAC 1 cut(s) 18
FatI CATG 4 cut(s) 51, 286, 340, 559
FauNDI CATATG 1 cut(s) 610
Fnu4HI GCNGC 4 cut(s) 71, 304, 307, 327
FokI GGATG 1 cut(s) 290
FriOI GRGCYC 1 cut(s) 784
Fsp4HI GCNGC 4 cut(s) 71, 304, 307, 327
FspBI CTAG 1 cut(s) 452
GlaI GCGC 1 cut(s) 865
GluI GCNGC 4 cut(s) 71, 304, 307, 327
GsuI CTGGAG 1 cut(s) 505
HaeII RGCGCY 1 cut(s) 867
HaeIII GGCC 6 cut(s) 81, 156, 174, 356, 930, 961
HapII CCGG 2 cut(s) 117, 357
HhaI GCGC 1 cut(s) 866
Hin1II CATG 4 cut(s) 55, 290, 344, 563
Hin6I GCGC 1 cut(s) 864
HinP1I GCGC 1 cut(s) 864
HincII GTYRAC 1 cut(s) 800
HindII GTYRAC 1 cut(s) 800
HinfI GANTC 4 cut(s) 188, 485, 638, 676
HpaII CCGG 2 cut(s) 117, 357
HphI GGTGA 1 cut(s) 699
Hpy166II GTNNAC 3 cut(s) 163, 338, 800
Hpy188I TCNGA 4 cut(s) 586, 596, 671, 970
Hpy188III TCNNGA 4 cut(s) 25, 52, 287, 757
Hpy8I GTNNAC 3 cut(s) 163, 338, 800
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 4 cut(s) 189, 230, 963, 972
HpyCH4III ACNGT 2 cut(s) 879, 919
HpyCH4IV ACGT 2 cut(s) 752, 832
HpyCH4V TGCA 4 cut(s) 108, 317, 539, 899
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 323
HpyF3I CTNAG 4 cut(s) 66, 430, 440, 934
HpySE526I ACGT 2 cut(s) 752, 832
Hsp92II CATG 4 cut(s) 55, 290, 344, 563
HspAI GCGC 1 cut(s) 864
KroI GCCGGC 1 cut(s) 356
KroNI GCCGGC 1 cut(s) 358
Kzo9I GATC 7 cut(s) 199, 448, 513, 666, 704, 785, 970
LmnI GCTCC 2 cut(s) 524, 831
Lsp1109I GCAGC 3 cut(s) 315, 318, 338
LweI GCATC 1 cut(s) 271
MaeI CTAG 1 cut(s) 452
MaeII ACGT 2 cut(s) 752, 832
MaeIII GTNAC 1 cut(s) 35
MalI GATC 7 cut(s) 201, 450, 515, 668, 706, 787, 972
MboI GATC 7 cut(s) 199, 448, 513, 666, 704, 785, 970
MboII GAAGA 6 cut(s) 133, 233, 401, 487, 640, 731
MfeI CAATTG 1 cut(s) 261
MflI RGATCY 1 cut(s) 970
MhlI GDGCHC 1 cut(s) 784
MlsI TGGCCA 1 cut(s) 174
MluCI AATT 6 cut(s) 101, 261, 461, 614, 768, 803
MluNI TGGCCA 1 cut(s) 174
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 9 cut(s) 59, 67, 92, 265, 268, 316, 471, 624, 884
Mox20I TGGCCA 1 cut(s) 174
MroNI GCCGGC 1 cut(s) 356
MscI TGGCCA 1 cut(s) 174
MseI TTAA 1 cut(s) 767
MslI CAYNNNNRTG 1 cut(s) 291
Msp20I TGGCCA 1 cut(s) 174
MspA1I CMGCKG 1 cut(s) 329
MspI CCGG 2 cut(s) 117, 357
MspR9I CCNGG 1 cut(s) 150
MunI CAATTG 1 cut(s) 261
MvaI CCWGG 1 cut(s) 150
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 2 cut(s) 128, 323
NaeI GCCGGC 1 cut(s) 358
NdeI CATATG 1 cut(s) 610
NdeII GATC 7 cut(s) 199, 448, 513, 666, 704, 785, 970
NgoMIV GCCGGC 1 cut(s) 356
NlaIII CATG 4 cut(s) 55, 290, 344, 563
NlaIV GGNNCC 3 cut(s) 121, 520, 827
PagI TCATGA 2 cut(s) 51, 286
PceI AGGCCT 1 cut(s) 961
PdiI GCCGGC 1 cut(s) 358
PfeI GAWTC 3 cut(s) 485, 638, 676
PflFI GACNNNGTC 1 cut(s) 191
PkrI GCNGC 4 cut(s) 72, 305, 308, 328
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
Ppu21I YACGTR 1 cut(s) 833
Psp1406I AACGTT 1 cut(s) 752
Psp6I CCWGG 1 cut(s) 148
PspGI CCWGG 1 cut(s) 148
PspN4I GGNNCC 3 cut(s) 121, 520, 827
PspPI GGNCC 2 cut(s) 928, 950
PsuI RGATCY 1 cut(s) 970
PsyI GACNNNGTC 1 cut(s) 191
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 3 cut(s) 835, 873, 921
RsaNI GTAC 3 cut(s) 834, 872, 920
RseI CAYNNNNRTG 1 cut(s) 291
SaqAI TTAA 1 cut(s) 767
SatI GCNGC 4 cut(s) 71, 304, 307, 327
Sau3AI GATC 7 cut(s) 199, 448, 513, 666, 704, 785, 970
Sau96I GGNCC 2 cut(s) 928, 950
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 1 cut(s) 150
SduI GDGCHC 1 cut(s) 784
SfaNI GCATC 1 cut(s) 271
SfcI CTRYAG 2 cut(s) 712, 915
SinI GGWCC 1 cut(s) 950
SmiMI CAYNNNNRTG 1 cut(s) 291
SmlI CTYRAG 2 cut(s) 410, 757
SmoI CTYRAG 2 cut(s) 410, 757
SnaBI TACGTA 1 cut(s) 833
Sse9I AATT 6 cut(s) 101, 261, 461, 614, 768, 803
SseBI AGGCCT 1 cut(s) 961
SsiI CCGC 3 cut(s) 70, 131, 816
SspMI CTAG 1 cut(s) 452
StuI AGGCCT 1 cut(s) 961
StyD4I CCNGG 1 cut(s) 148
TaaI ACNGT 2 cut(s) 879, 919
TaiI ACGT 2 cut(s) 755, 835
TaqI TCGA 1 cut(s) 784
TasI AATT 6 cut(s) 101, 261, 461, 614, 768, 803
TatI WGTACW 2 cut(s) 871, 919
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 3 cut(s) 485, 638, 676
Tru1I TTAA 1 cut(s) 767
Tru9I TTAA 1 cut(s) 767
TseI GCWGC 3 cut(s) 303, 306, 326
TspDTI ATGAA 2 cut(s) 249, 627
TspGWI ACGGA 2 cut(s) 22, 181
Tth111I GACNNNGTC 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 950
XagI CCTNNNNNAGG 1 cut(s) 888
XspI CTAG 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.