Rw2G027380
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000806
Physical Location & Seq
Forward (+)
42846097 .. 42849199
3103 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027380.1

Sequence Viewer

Length: 678 bp
ATGGGACGCAGGAAGATAGAAATCGAGAAAATCAGTAACAAGAACTACCTAATGGCCACATTCTCTAAGCGGCGAAAGGGTCTGTTTCACAAAGCCAGTGAGTTTTGCAGGTTATCCGGTGCCGAAATCGCCATCGTTGTCTTTTCGCCAGGTGACCGTGTTTACCGGTTTGGTCACCCTTCAGCAGACTCCATCATTGACCGGTATGATGATCCGCGAACCGAAAATAACAATAATAAGGGCACACGAGAGTTTCCGTATACACTTGACGACGAAGGCAATGAAGCTCATGATGATGATTTGGCAGGCAGCAAGCTTGGAAAAGAGGCAGCTGATGGTAAGGATGAGCTTGGAAAGGCCAGGTTTTGGTGGGATAATCTGCCTATTGAAGATATGGGACTAAAACAACTTGAGCAATATAAAAGTTGCTTAGAAATTATAAGAAACGAGCTGTGGTCTCACTTCGACTTTAGTGGTCCAACTTCTGGGAGCACAGCTTGTGTTGCAATTATTAGAAACAACCAACTTGTTGTTGCCAATGCTGGTGATTCTCGTTGTGTCATATTGAGGAAGGGTCAGATAGGAGATCAAAGATGTGCTAAATTCACACTCCATTTTGTCCAGCTTAGGGAAAGGGTGATGCTACAACTTAAGCTTGGCAAAATCACTGCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04013 ko04022 ko04111 ko04371 ko04921 ko05202 ko05418 map04010 map04011 map04013 map04022 map04111 map04371 map04921 map05202 map05418
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

225

Amino Acids

25.5

Weight (kDa)

8.54

Isoelectric Point (pI)

39.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 5e-18 SRF-type transcription factor (DNA-binding and dimerisation domain)
PP2C PF00481 158 - 195 7e-10 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 440
Acc36I ACCTGC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 119
AccB7I CCANNNNNTGG 2 cut(s) 366, 485
AccI GTMKAC 1 cut(s) 260
AccII CGCG 1 cut(s) 217
AciI CCGC 2 cut(s) 70, 215
AclWI GGATC 1 cut(s) 206
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 602
AcuI CTGAAG 1 cut(s) 165
AfiI CCNNNNNNNGG 3 cut(s) 366, 485, 628
AflII CTTAAG 1 cut(s) 650
AgeI ACCGGT 2 cut(s) 165, 201
AgsI TTSAA 1 cut(s) 389
AjnI CCWGG 2 cut(s) 148, 359
AluBI AGCT 8 cut(s) 287, 316, 332, 349, 451, 497, 625, 655
AluI AGCT 8 cut(s) 287, 316, 332, 349, 451, 497, 625, 655
Alw21I GWGCWC 1 cut(s) 494
Alw26I GTCTC 1 cut(s) 462
AlwI GGATC 1 cut(s) 206
AoxI GGCC 2 cut(s) 54, 357
ApeKI GCWGC 2 cut(s) 309, 329
ApoI RAATTY 1 cut(s) 602
AsiGI ACCGGT 2 cut(s) 165, 201
AspS9I GGNCC 1 cut(s) 476
AsuHPI GGTGA 4 cut(s) 164, 167, 557, 649
AvaII GGWCC 1 cut(s) 476
BaeGI GKGCMC 1 cut(s) 245
BalI TGGCCA 1 cut(s) 56
BanI GGYRCC 1 cut(s) 119
BauI CACGAG 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 494
BbvI GCAGC 2 cut(s) 321, 341
BccI CCATC 3 cut(s) 140, 200, 329
BciT130I CCWGG 2 cut(s) 150, 361
BcoDI GTCTC 1 cut(s) 462
BfrI CTTAAG 1 cut(s) 650
BfuAI ACCTGC 1 cut(s) 99
BisI GCNGC 3 cut(s) 71, 310, 330
BlsI GCNGC 3 cut(s) 72, 311, 331
Bme1390I CCNGG 2 cut(s) 150, 361
Bme18I GGWCC 1 cut(s) 476
BmgT120I GGNCC 1 cut(s) 476
BmiI GGNNCC 1 cut(s) 121
BmrFI CCNGG 2 cut(s) 150, 361
BmsI GCATC 1 cut(s) 630
Bpu10I CCTNAGC 1 cut(s) 626
BpuEI CTTGAG 1 cut(s) 431
BsaBI GATNNNNATC 1 cut(s) 20
BsaI GGTCTC 1 cut(s) 462
BsaWI WCCGGW 3 cut(s) 116, 165, 201
Bsc4I CCNNNNNNNGG 3 cut(s) 366, 485, 628
Bse118I RCCGGY 2 cut(s) 165, 201
Bse1I ACTGG 1 cut(s) 96
Bse3DI GCAATG 1 cut(s) 286
Bse8I GATNNNNATC 1 cut(s) 20
BseBI CCWGG 2 cut(s) 150, 361
BseGI GGATG 1 cut(s) 349
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 3 cut(s) 366, 485, 628
BseMI GCAATG 1 cut(s) 286
BseNI ACTGG 1 cut(s) 96
BseSI GKGCMC 1 cut(s) 245
BseXI GCAGC 2 cut(s) 321, 341
Bsh1236I CGCG 1 cut(s) 217
BshFI GGCC 2 cut(s) 56, 359
BshNI GGYRCC 1 cut(s) 119
BshTI ACCGGT 2 cut(s) 165, 201
BsiHKAI GWGCWC 1 cut(s) 494
BsiSI CCGG 3 cut(s) 117, 166, 202
BslFI GGGAC 2 cut(s) 18, 411
BslI CCNNNNNNNGG 3 cut(s) 366, 485, 628
BsmAI GTCTC 1 cut(s) 462
BsmFI GGGAC 2 cut(s) 18, 411
BsnI GGCC 2 cut(s) 56, 359
Bso31I GGTCTC 1 cut(s) 462
Bsp1286I GDGCHC 2 cut(s) 245, 494
Bsp143I GATC 2 cut(s) 211, 586
BspACI CCGC 2 cut(s) 70, 215
BspANI GGCC 2 cut(s) 56, 359
BspFNI CGCG 1 cut(s) 217
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 1 cut(s) 121
BspMI ACCTGC 1 cut(s) 99
BspPI GGATC 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 119
BspTI CTTAAG 1 cut(s) 650
BspTNI GGTCTC 1 cut(s) 462
BsrDI GCAATG 1 cut(s) 286
BsrFI RCCGGY 2 cut(s) 165, 201
BsrI ACTGG 1 cut(s) 96
BssAI RCCGGY 2 cut(s) 165, 201
BssMI GATC 2 cut(s) 211, 586
BssNAI GTATAC 1 cut(s) 261
BssSI CACGAG 1 cut(s) 246
Bst1107I GTATAC 1 cut(s) 261
Bst2BI CACGAG 1 cut(s) 246
Bst2UI CCWGG 2 cut(s) 150, 361
Bst4CI ACNGT 1 cut(s) 158
BstAFI CTTAAG 1 cut(s) 650
BstC8I GCNNGC 2 cut(s) 307, 314
BstDEI CTNAG 3 cut(s) 66, 430, 626
BstEII GGTNACC 2 cut(s) 152, 173
BstF5I GGATG 1 cut(s) 349
BstFNI CGCG 1 cut(s) 217
BstKTI GATC 2 cut(s) 214, 589
BstMAI GTCTC 1 cut(s) 462
BstMBI GATC 2 cut(s) 211, 586
BstMWI GCNNNNNNNGC 2 cut(s) 128, 503
BstNI CCWGG 2 cut(s) 150, 361
BstPI GGTNACC 2 cut(s) 152, 173
BstSCI CCNGG 2 cut(s) 148, 359
BstSLI GKGCMC 1 cut(s) 245
BstUI CGCG 1 cut(s) 217
BstV1I GCAGC 2 cut(s) 321, 341
BstZ17I GTATAC 1 cut(s) 261
BsuRI GGCC 2 cut(s) 56, 359
BtsCI GGATG 1 cut(s) 349
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 2 cut(s) 103, 666
BveI ACCTGC 1 cut(s) 99
Cac8I GCNNGC 2 cut(s) 307, 314
CciI TCATGA 1 cut(s) 289
Cfr10I RCCGGY 2 cut(s) 165, 201
Cfr13I GGNCC 1 cut(s) 476
CseI GACGC 1 cut(s) 15
CspAI ACCGGT 2 cut(s) 165, 201
CviAII CATG 1 cut(s) 290
DdeI CTNAG 3 cut(s) 66, 430, 626
DpnI GATC 2 cut(s) 213, 588
DpnII GATC 2 cut(s) 211, 586
EaeI YGGCCR 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 462
Eco47I GGWCC 1 cut(s) 476
Eco57I CTGAAG 1 cut(s) 165
Eco91I GGTNACC 2 cut(s) 152, 173
EcoO65I GGTNACC 2 cut(s) 152, 173
EcoRII CCWGG 2 cut(s) 148, 359
FaeI CATG 1 cut(s) 293
FaiI YATR 7 cut(s) 207, 261, 291, 395, 420, 440, 563
FaqI GGGAC 2 cut(s) 18, 411
FatI CATG 1 cut(s) 289
FblI GTMKAC 1 cut(s) 260
Fnu4HI GCNGC 3 cut(s) 71, 310, 330
FokI GGATG 1 cut(s) 356
Fsp4HI GCNGC 3 cut(s) 71, 310, 330
GluI GCNGC 3 cut(s) 71, 310, 330
HaeIII GGCC 2 cut(s) 56, 359
HapII CCGG 3 cut(s) 117, 166, 202
HgaI GACGC 1 cut(s) 15
Hin1II CATG 1 cut(s) 293
HindIII AAGCTT 2 cut(s) 314, 653
HinfI GANTC 2 cut(s) 188, 548
HpaII CCGG 3 cut(s) 117, 166, 202
HphI GGTGA 4 cut(s) 164, 167, 557, 649
Hpy166II GTNNAC 2 cut(s) 163, 261
Hpy188I TCNGA 1 cut(s) 579
Hpy188III TCNNGA 2 cut(s) 25, 290
Hpy8I GTNNAC 2 cut(s) 163, 261
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 3 cut(s) 189, 269, 565
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4V TGCA 2 cut(s) 108, 506
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 503
HpyF3I CTNAG 3 cut(s) 66, 430, 626
Hsp92II CATG 1 cut(s) 293
Kzo9I GATC 2 cut(s) 211, 586
LmnI GCTCC 1 cut(s) 489
Lsp1109I GCAGC 2 cut(s) 321, 341
LweI GCATC 1 cut(s) 630
MaeIII GTNAC 3 cut(s) 35, 152, 173
MalI GATC 2 cut(s) 213, 588
MboI GATC 2 cut(s) 211, 586
MboII GAAGA 2 cut(s) 25, 401
MhlI GDGCHC 2 cut(s) 245, 494
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 3 cut(s) 435, 507, 602
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 503
MnlI CCTC 2 cut(s) 319, 561
Mox20I TGGCCA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 56
MseI TTAA 1 cut(s) 651
MslI CAYNNNNRTG 1 cut(s) 294
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 332
MspCI CTTAAG 1 cut(s) 650
MspI CCGG 3 cut(s) 117, 166, 202
MspR9I CCNGG 2 cut(s) 150, 361
MvaI CCWGG 2 cut(s) 150, 361
MvnI CGCG 1 cut(s) 217
MwoI GCNNNNNNNGC 2 cut(s) 128, 503
NdeII GATC 2 cut(s) 211, 586
NlaIII CATG 1 cut(s) 293
NlaIV GGNNCC 1 cut(s) 121
NmuCI GTSAC 2 cut(s) 152, 173
PagI TCATGA 1 cut(s) 289
PfeI GAWTC 1 cut(s) 548
PflMI CCANNNNNTGG 2 cut(s) 366, 485
PinAI ACCGGT 2 cut(s) 165, 201
PkrI GCNGC 3 cut(s) 72, 311, 331
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
PsiI TTATAA 1 cut(s) 440
Psp6I CCWGG 2 cut(s) 148, 359
PspEI GGTNACC 2 cut(s) 152, 173
PspGI CCWGG 2 cut(s) 148, 359
PspN4I GGNNCC 1 cut(s) 121
PspPI GGNCC 1 cut(s) 476
PvuII CAGCTG 1 cut(s) 332
RseI CAYNNNNRTG 1 cut(s) 294
SaqAI TTAA 1 cut(s) 651
SatI GCNGC 3 cut(s) 71, 310, 330
Sau3AI GATC 2 cut(s) 211, 586
Sau96I GGNCC 1 cut(s) 476
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 150, 361
SduI GDGCHC 2 cut(s) 245, 494
SfaNI GCATC 1 cut(s) 630
SinI GGWCC 1 cut(s) 476
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 2 cut(s) 410, 650
SmoI CTYRAG 2 cut(s) 410, 650
Sse9I AATT 3 cut(s) 435, 507, 602
SsiI CCGC 2 cut(s) 70, 215
StyD4I CCNGG 2 cut(s) 148, 359
TaaI ACNGT 1 cut(s) 158
TaqI TCGA 2 cut(s) 24, 465
TasI AATT 3 cut(s) 435, 507, 602
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 1 cut(s) 651
Tru9I TTAA 1 cut(s) 651
TscAI CASTG 2 cut(s) 103, 673
TseFI GTSAC 2 cut(s) 152, 173
TseI GCWGC 2 cut(s) 309, 329
Tsp45I GTSAC 2 cut(s) 152, 173
TspDTI ATGAA 1 cut(s) 297
TspGWI ACGGA 1 cut(s) 246
TspRI CASTG 2 cut(s) 103, 673
Van91I CCANNNNNTGG 2 cut(s) 366, 485
Vha464I CTTAAG 1 cut(s) 650
VpaK11BI GGWCC 1 cut(s) 476
XapI RAATTY 1 cut(s) 602
XmiI GTMKAC 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.