Rroxscaffold_2G00114730
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000806
Physical Location & Seq
Reverse (-)
40870793 .. 40871341
549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00114730.1

Sequence Viewer

Length: 549 bp
ATGGGACGCAGGAAGATAGAAATCGAGAAAATCAGTAACAAGAACTACCTAATGGCCACATTCTCCAAGCGGCGAAAGGGTCTGTTTCACAAAGCCAGTGAGTTTTGCAGGTTATCCGGTGCCGAAATCGCCATCATTGTCTTTTCGCCAGGTGGCCGTGTTTACCGGTTTGGTCACCCTTCGGCAGACTCCATCATTGATCGCTATGATGATCCGCGAACTGAAAATATCAATAATAAGGGCACACGAGAGTTTCCGTATACACTTGACGACGAAGACAATGAAGCTCATGATGATGATTTGGCGGGCGGCGAGCTTGGAAAAGAGGCAGCTGATGGTAAGGATGAGCTTGGAAAGGCCACGTTTTGGTGGGATAATCTGCCTATTGAAGATATGGGACTAAAACAACTTGAGCAATATAAAAGTTGCTTAGAAGTTATAAGAAACGAGCTGGCATATGAATTACATGAGACGAAGAGAAGAGAATCACACACCAAAGATTTTTTGAGTTTGATTGGCTTCGGTACTAGTACTTCTACTTCGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04013 ko04022 ko04111 ko04371 ko04921 ko05202 ko05418 map04010 map04011 map04013 map04022 map04111 map04371 map04921 map05202 map05418
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

182

Amino Acids

20.76

Weight (kDa)

5.46

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 5e-19 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 440
Acc36I ACCTGC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 119
AccB7I CCANNNNNTGG 1 cut(s) 366
AccI GTMKAC 1 cut(s) 260
AccII CGCG 1 cut(s) 217
AciI CCGC 4 cut(s) 70, 215, 305, 309
AclWI GGATC 1 cut(s) 206
AcoI YGGCCR 2 cut(s) 54, 154
AfaI GTAC 2 cut(s) 526, 532
AfiI CCNNNNNNNGG 1 cut(s) 366
AgeI ACCGGT 1 cut(s) 165
AgsI TTSAA 1 cut(s) 389
AhlI ACTAGT 1 cut(s) 527
AjnI CCWGG 1 cut(s) 148
AluBI AGCT 5 cut(s) 287, 316, 332, 349, 451
AluI AGCT 5 cut(s) 287, 316, 332, 349, 451
Alw26I GTCTC 1 cut(s) 464
AlwI GGATC 1 cut(s) 206
AoxI GGCC 3 cut(s) 54, 154, 357
ApeKI GCWGC 1 cut(s) 329
AsiGI ACCGGT 1 cut(s) 165
AsuHPI GGTGA 1 cut(s) 167
BaeGI GKGCMC 1 cut(s) 245
BalI TGGCCA 1 cut(s) 56
BanI GGYRCC 1 cut(s) 119
BarI GAAGNNNNNNTAC 3 cut(s) 517, 523, 549
BauI CACGAG 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 282
BbvI GCAGC 1 cut(s) 341
BccI CCATC 3 cut(s) 140, 200, 329
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 464
BcuI ACTAGT 1 cut(s) 527
BfaI CTAG 1 cut(s) 528
BfuAI ACCTGC 1 cut(s) 99
BisI GCNGC 3 cut(s) 71, 310, 330
BlsI GCNGC 3 cut(s) 72, 311, 331
BmcAI AGTACT 1 cut(s) 532
Bme1390I CCNGG 1 cut(s) 150
BmiI GGNNCC 1 cut(s) 121
BmrFI CCNGG 1 cut(s) 150
BpiI GAAGAC 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 431
BsaBI GATNNNNATC 1 cut(s) 20
BsaWI WCCGGW 2 cut(s) 116, 165
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse118I RCCGGY 1 cut(s) 165
Bse1I ACTGG 1 cut(s) 96
Bse8I GATNNNNATC 1 cut(s) 20
BseBI CCWGG 1 cut(s) 150
BseGI GGATG 1 cut(s) 349
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 1 cut(s) 366
BseNI ACTGG 1 cut(s) 96
BseSI GKGCMC 1 cut(s) 245
BseXI GCAGC 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 217
BshFI GGCC 3 cut(s) 56, 156, 359
BshNI GGYRCC 1 cut(s) 119
BshTI ACCGGT 1 cut(s) 165
BsiSI CCGG 2 cut(s) 117, 166
BslFI GGGAC 2 cut(s) 18, 411
BslI CCNNNNNNNGG 1 cut(s) 366
BsmAI GTCTC 1 cut(s) 464
BsmBI CGTCTC 1 cut(s) 464
BsmFI GGGAC 2 cut(s) 18, 411
BsnI GGCC 3 cut(s) 56, 156, 359
Bsp1286I GDGCHC 1 cut(s) 245
Bsp143I GATC 3 cut(s) 199, 211, 543
BspACI CCGC 4 cut(s) 70, 215, 305, 309
BspANI GGCC 3 cut(s) 56, 156, 359
BspFNI CGCG 1 cut(s) 217
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 1 cut(s) 121
BspMI ACCTGC 1 cut(s) 99
BspPI GGATC 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 119
BsrFI RCCGGY 1 cut(s) 165
BsrI ACTGG 1 cut(s) 96
BssAI RCCGGY 1 cut(s) 165
BssMI GATC 3 cut(s) 199, 211, 543
BssNAI GTATAC 1 cut(s) 261
BssSI CACGAG 1 cut(s) 246
Bst1107I GTATAC 1 cut(s) 261
Bst2BI CACGAG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 150
Bst6I CTCTTC 2 cut(s) 470, 475
BstC8I GCNNGC 3 cut(s) 307, 314, 453
BstDEI CTNAG 1 cut(s) 430
BstEII GGTNACC 1 cut(s) 173
BstF5I GGATG 1 cut(s) 349
BstFNI CGCG 1 cut(s) 217
BstKTI GATC 3 cut(s) 202, 214, 546
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 3 cut(s) 199, 211, 543
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstNI CCWGG 1 cut(s) 150
BstPI GGTNACC 1 cut(s) 173
BstSCI CCNGG 1 cut(s) 148
BstSLI GKGCMC 1 cut(s) 245
BstUI CGCG 1 cut(s) 217
BstV1I GCAGC 1 cut(s) 341
BstV2I GAAGAC 1 cut(s) 282
BstZ17I GTATAC 1 cut(s) 261
BsuRI GGCC 3 cut(s) 56, 156, 359
BtsCI GGATG 1 cut(s) 349
BtsIMutI CAGTG 1 cut(s) 103
BveI ACCTGC 1 cut(s) 99
Cac8I GCNNGC 3 cut(s) 307, 314, 453
CciI TCATGA 1 cut(s) 289
Cfr10I RCCGGY 1 cut(s) 165
CseI GACGC 1 cut(s) 15
Csp6I GTAC 2 cut(s) 525, 531
CspAI ACCGGT 1 cut(s) 165
CviAII CATG 2 cut(s) 290, 467
CviQI GTAC 2 cut(s) 525, 531
DdeI CTNAG 1 cut(s) 430
DpnI GATC 3 cut(s) 201, 213, 545
DpnII GATC 3 cut(s) 199, 211, 543
EaeI YGGCCR 2 cut(s) 54, 154
Eam1104I CTCTTC 2 cut(s) 470, 475
EarI CTCTTC 2 cut(s) 470, 475
Eco91I GGTNACC 1 cut(s) 173
EcoO65I GGTNACC 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 148
Esp3I CGTCTC 1 cut(s) 464
FaeI CATG 2 cut(s) 293, 470
FaiI YATR 9 cut(s) 207, 261, 291, 395, 420, 440, 457, 459, 468
FaqI GGGAC 2 cut(s) 18, 411
FatI CATG 2 cut(s) 289, 466
FauI CCCGC 1 cut(s) 298
FauNDI CATATG 1 cut(s) 457
FblI GTMKAC 1 cut(s) 260
Fnu4HI GCNGC 3 cut(s) 71, 310, 330
FokI GGATG 1 cut(s) 356
Fsp4HI GCNGC 3 cut(s) 71, 310, 330
FspBI CTAG 1 cut(s) 528
GluI GCNGC 3 cut(s) 71, 310, 330
HaeIII GGCC 3 cut(s) 56, 156, 359
HapII CCGG 2 cut(s) 117, 166
HgaI GACGC 1 cut(s) 15
Hin1II CATG 2 cut(s) 293, 470
HinfI GANTC 2 cut(s) 188, 485
HpaII CCGG 2 cut(s) 117, 166
HphI GGTGA 1 cut(s) 167
Hpy166II GTNNAC 2 cut(s) 163, 261
Hpy188I TCNGA 1 cut(s) 548
Hpy188III TCNNGA 2 cut(s) 25, 290
Hpy8I GTNNAC 2 cut(s) 163, 261
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 1 cut(s) 189
HpyCH4IV ACGT 1 cut(s) 362
HpyCH4V TGCA 1 cut(s) 108
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 1 cut(s) 430
HpySE526I ACGT 1 cut(s) 362
Hsp92II CATG 2 cut(s) 293, 470
Kzo9I GATC 3 cut(s) 199, 211, 543
LpnPI CCDG 7 cut(s) 94, 109, 130, 135, 162, 179, 437
Lsp1109I GCAGC 1 cut(s) 341
MaeI CTAG 1 cut(s) 528
MaeII ACGT 1 cut(s) 362
MaeIII GTNAC 2 cut(s) 35, 173
MalI GATC 3 cut(s) 201, 213, 545
MboI GATC 3 cut(s) 199, 211, 543
MboII GAAGA 5 cut(s) 25, 287, 401, 487, 492
MhlI GDGCHC 1 cut(s) 245
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 1 cut(s) 461
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 1 cut(s) 319
Mox20I TGGCCA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 56
MslI CAYNNNNRTG 1 cut(s) 294
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 332
MspI CCGG 2 cut(s) 117, 166
MspR9I CCNGG 1 cut(s) 150
MvaI CCWGG 1 cut(s) 150
MvnI CGCG 1 cut(s) 217
MwoI GCNNNNNNNGC 1 cut(s) 128
NdeI CATATG 1 cut(s) 457
NdeII GATC 3 cut(s) 199, 211, 543
NlaIII CATG 2 cut(s) 293, 470
NlaIV GGNNCC 1 cut(s) 121
NmuCI GTSAC 1 cut(s) 173
PagI TCATGA 1 cut(s) 289
PfeI GAWTC 1 cut(s) 485
PflMI CCANNNNNTGG 1 cut(s) 366
PinAI ACCGGT 1 cut(s) 165
PkrI GCNGC 3 cut(s) 72, 311, 331
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
PsiI TTATAA 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 148
PspEI GGTNACC 1 cut(s) 173
PspGI CCWGG 1 cut(s) 148
PspN4I GGNNCC 1 cut(s) 121
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 2 cut(s) 526, 532
RsaNI GTAC 2 cut(s) 525, 531
RseI CAYNNNNRTG 1 cut(s) 294
SatI GCNGC 3 cut(s) 71, 310, 330
Sau3AI GATC 3 cut(s) 199, 211, 543
ScaI AGTACT 1 cut(s) 532
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 1 cut(s) 150
SduI GDGCHC 1 cut(s) 245
SetI ASST 9 cut(s) 51, 113, 154, 289, 318, 334, 351, 365, 453
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
SpeI ACTAGT 1 cut(s) 527
Sse9I AATT 1 cut(s) 461
SsiI CCGC 4 cut(s) 70, 215, 305, 309
SspMI CTAG 1 cut(s) 528
StyD4I CCNGG 1 cut(s) 148
TaiI ACGT 1 cut(s) 365
TaqI TCGA 2 cut(s) 24, 542
TasI AATT 1 cut(s) 461
TatI WGTACW 1 cut(s) 530
TauI GCSGC 2 cut(s) 73, 312
TfiI GAWTC 1 cut(s) 485
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 1 cut(s) 329
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 2 cut(s) 297, 474
TspGWI ACGGA 1 cut(s) 246
TspRI CASTG 1 cut(s) 103
Van91I CCANNNNNTGG 1 cut(s) 366
XmiI GTMKAC 1 cut(s) 260
XspI CTAG 1 cut(s) 528
ZrmI AGTACT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.