MD01G1095300.v1.1

Ribosome production factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002361
Physical Location & Seq
Reverse (-)
21037297 .. 21040356
3060 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1095300.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGGAGGCGATAGTAACAAGAGGAAGCGAAGCGAAAGCGACGGAGGAGGAGAAGAACAACCGCCGGAGAGGAAGATGGAGATTCTGCCGTCGATGATAAAGAACAAGGAGAAGAGGTCGGCGGTCCACGCCAAGCTCAAGCAGCAGAAGAAGCTTGAGAAGCGCAAGAAGGCCAAGGCCCGTGACGCTGCTGAGAAGCGCGCTCTCGAGCTCGGCGAGGAGCCCCCGGCAAAGAAGATCCCGAAGACGATTGAGAACACCAGGGAAGTCGATGAGACTGTTTGTAAGCCCGACGATGAAGAGCTGTTTGCTGGAAATGATGCCGATGAATTCAGTTCGATTTTAAAGCGCGAATCGAATCCAAAGATATTAATCACCACTTGCCGTTTCAATTCTTCTAGGGGGCCTGAGTTTATAAAGGAACTACTCTCCGTGATCCCAAATGCAACCTACTATAAAAGAGGAACATATGACTTGAAAAAGATTGTAGAGTACGCAAATAAGAAAGACTTCACTTCTCTAATTGTTGTGCACACCAATCGCAGGGAACCAGATGCTCTCCTAATAATTGGCTTACCTGCTGGGCCTACTGCCCATTTCAAGCTCTCAAAGCTTGTTTTACGCAAGGATATCAAGAATCATGGAAATCCAACCAGTCATGAGCCCGAGCTTGTATTAAACAACTTTACAACACGCCTTGGTCATCGTATAGGAAGATTAATACAGTCACTTTTCCCACAAGAACCAAACTTCCGTGGTCGGCGAGTTGTAACTTTTCACAACCAGAGAGATTTTATATTCTTCCGGCATCATCGGTATATCTTTGAAGAAAAAGAGACTAAACAGATAGAGTCCAAAGGTAAAAAGACCAAGGATGCAAATGCTGAGAGCGGACCTAATGGGAAAGTAATTGCCCGCCTACAGGAGTGTGGCCCTCGTTTCACCCTTAAACTAATTAGTCTGCAGCATGGGACCTTCGATACTAAAGGCGGGGAATATGAGTGGGTTCACAAGCCGGAAATGGACACTAGCAGAAGGAGATTTTTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Brix

Protein Analysis

351

Amino Acids

40.27

Weight (kDa)

9.73

Isoelectric Point (pI)

50.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Brix PF04427 131 - 319 5.5e-34 Brix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 416
Acc36I ACCTGC 1 cut(s) 586
AccBSI CCGCTC 1 cut(s) 891
AccII CGCG 2 cut(s) 201, 351
AciI CCGC 5 cut(s) 62, 122, 891, 916, 990
AclWI GGATC 2 cut(s) 232, 430
AcsI RAATTY 1 cut(s) 329
AfaI GTAC 1 cut(s) 494
AfiI CCNNNNNNNGG 2 cut(s) 543, 922
AgsI TTSAA 4 cut(s) 391, 478, 601, 827
AjnI CCWGG 1 cut(s) 260
AloI GAACNNNNNNTCC 2 cut(s) 735, 767
AluBI AGCT 7 cut(s) 136, 154, 211, 304, 604, 613, 670
AluI AGCT 7 cut(s) 136, 154, 211, 304, 604, 613, 670
Alw21I GWGCWC 2 cut(s) 213, 534
Alw26I GTCTC 2 cut(s) 269, 830
Alw44I GTGCAC 1 cut(s) 530
AlwI GGATC 2 cut(s) 232, 430
Ama87I CYCGRG 2 cut(s) 206, 665
AoxI GGCC 5 cut(s) 171, 177, 404, 584, 931
ApaLI GTGCAC 1 cut(s) 530
ApeKI GCWGC 3 cut(s) 142, 188, 964
ApoI RAATTY 1 cut(s) 329
AseI ATTAAT 2 cut(s) 371, 719
Asp700I GAANNNNTTC 1 cut(s) 509
AspLEI GCGC 4 cut(s) 165, 201, 203, 351
AspS9I GGNCC 7 cut(s) 124, 178, 404, 584, 893, 932, 972
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 367, 934
AvaI CYCGRG 2 cut(s) 206, 665
AvaII GGWCC 3 cut(s) 124, 893, 972
BaeGI GKGCMC 1 cut(s) 534
BanII GRGCYC 3 cut(s) 213, 225, 666
BbsI GAAGAC 1 cut(s) 251
Bbv12I GWGCWC 2 cut(s) 213, 534
BbvI GCAGC 3 cut(s) 154, 175, 976
BccI CCATC 1 cut(s) 70
BceAI ACGGC 2 cut(s) 73, 369
BcgI CGANNNNNNTGC 2 cut(s) 521, 555
BciT130I CCWGG 1 cut(s) 262
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 2 cut(s) 269, 830
BfaI CTAG 2 cut(s) 399, 1029
BfmI CTRYAG 2 cut(s) 920, 962
BfuAI ACCTGC 1 cut(s) 586
BisI GCNGC 3 cut(s) 143, 189, 965
BlsI GCNGC 3 cut(s) 144, 190, 966
Bme1390I CCNGG 2 cut(s) 227, 262
Bme18I GGWCC 3 cut(s) 124, 893, 972
BmeT110I CYCGRG 2 cut(s) 206, 665
BmgT120I GGNCC 7 cut(s) 124, 178, 404, 584, 893, 932, 972
BmiI GGNNCC 4 cut(s) 222, 405, 549, 973
BmrFI CCNGG 2 cut(s) 227, 262
BmsI GCATC 4 cut(s) 310, 544, 817, 865
BpiI GAAGAC 1 cut(s) 251
BpuEI CTTGAG 2 cut(s) 122, 176
BpuMI CCSGG 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 371
BsaJI CCNNGG 6 cut(s) 174, 225, 261, 697, 754, 870
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 2 cut(s) 543, 922
Bse1I ACTGG 1 cut(s) 654
Bse8I GATNNNNATC 1 cut(s) 371
BseBI CCWGG 1 cut(s) 262
BseDI CCNNGG 6 cut(s) 174, 225, 261, 697, 754, 870
BseGI GGATG 1 cut(s) 880
BseJI GATNNNNATC 1 cut(s) 371
BseLI CCNNNNNNNGG 2 cut(s) 543, 922
BseMII CTCAG 3 cut(s) 183, 399, 876
BseNI ACTGG 1 cut(s) 654
BsePI GCGCGC 1 cut(s) 199
BseRI GAGGAG 3 cut(s) 60, 63, 233
BseSI GKGCMC 1 cut(s) 534
BseXI GCAGC 3 cut(s) 154, 175, 976
BseYI CCCAGC 1 cut(s) 581
Bsh1236I CGCG 2 cut(s) 201, 351
BshFI GGCC 5 cut(s) 173, 179, 406, 586, 933
BsiHKAI GWGCWC 2 cut(s) 213, 534
BsiHKCI CYCGRG 2 cut(s) 206, 665
BsiSI CCGG 4 cut(s) 65, 227, 805, 1016
BslFI GGGAC 1 cut(s) 985
BslI CCNNNNNNNGG 2 cut(s) 543, 922
BsmAI GTCTC 2 cut(s) 269, 830
BsmFI GGGAC 1 cut(s) 985
BsnI GGCC 5 cut(s) 173, 179, 406, 586, 933
BsoBI CYCGRG 2 cut(s) 206, 665
Bsp1286I GDGCHC 4 cut(s) 213, 225, 534, 666
Bsp143I GATC 2 cut(s) 237, 435
BspACI CCGC 5 cut(s) 62, 122, 891, 916, 990
BspANI GGCC 5 cut(s) 173, 179, 406, 586, 933
BspCNI CTCAG 3 cut(s) 184, 400, 877
BspFNI CGCG 2 cut(s) 201, 351
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 4 cut(s) 222, 405, 549, 973
BspMAI CTGCAG 1 cut(s) 966
BspMI ACCTGC 1 cut(s) 586
BspPI GGATC 2 cut(s) 232, 430
BspQI GCTCTTC 1 cut(s) 294
BsrBI CCGCTC 1 cut(s) 891
BsrI ACTGG 1 cut(s) 654
BssECI CCNNGG 6 cut(s) 174, 225, 261, 697, 754, 870
BssHII GCGCGC 1 cut(s) 199
BssMI GATC 2 cut(s) 237, 435
BssT1I CCWWGG 3 cut(s) 174, 697, 870
Bst2UI CCWGG 1 cut(s) 262
Bst4CI ACNGT 2 cut(s) 280, 726
Bst6I CTCTTC 2 cut(s) 107, 294
BstC8I GCNNGC 2 cut(s) 201, 916
BstDEI CTNAG 3 cut(s) 192, 408, 885
BstDSI CCRYGG 1 cut(s) 754
BstF5I GGATG 1 cut(s) 880
BstFNI CGCG 2 cut(s) 201, 351
BstHHI GCGC 4 cut(s) 165, 201, 203, 351
BstKTI GATC 2 cut(s) 240, 438
BstMAI GTCTC 2 cut(s) 269, 830
BstMBI GATC 2 cut(s) 237, 435
BstMWI GCNNNNNNNGC 6 cut(s) 128, 142, 151, 160, 185, 610
BstNI CCWGG 1 cut(s) 262
BstSCI CCNGG 2 cut(s) 225, 260
BstSFI CTRYAG 2 cut(s) 920, 962
BstSLI GKGCMC 1 cut(s) 534
BstUI CGCG 2 cut(s) 201, 351
BstV1I GCAGC 3 cut(s) 154, 175, 976
BstV2I GAAGAC 1 cut(s) 251
BstX2I RGATCY 1 cut(s) 237
BstYI RGATCY 1 cut(s) 237
BsuRI GGCC 5 cut(s) 173, 179, 406, 586, 933
BtgI CCRYGG 1 cut(s) 754
BtsCI GGATG 1 cut(s) 880
BveI ACCTGC 1 cut(s) 586
Cac8I GCNNGC 2 cut(s) 201, 916
CciI TCATGA 1 cut(s) 658
CfoI GCGC 4 cut(s) 165, 201, 203, 351
Cfr13I GGNCC 7 cut(s) 124, 178, 404, 584, 893, 932, 972
CseI GACGC 1 cut(s) 194
Csp6I GTAC 1 cut(s) 493
CviAII CATG 3 cut(s) 641, 659, 968
CviQI GTAC 1 cut(s) 493
DdeI CTNAG 3 cut(s) 192, 408, 885
DpnI GATC 2 cut(s) 239, 437
DpnII GATC 2 cut(s) 237, 435
DraI TTTAAA 1 cut(s) 345
Eam1104I CTCTTC 2 cut(s) 107, 294
EarI CTCTTC 2 cut(s) 107, 294
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 3 cut(s) 174, 697, 870
Eco24I GRGCYC 3 cut(s) 213, 225, 666
Eco32I GATATC 1 cut(s) 631
Eco47I GGWCC 3 cut(s) 124, 893, 972
Eco53kI GAGCTC 1 cut(s) 211
Eco88I CYCGRG 2 cut(s) 206, 665
EcoICRI GAGCTC 1 cut(s) 211
EcoO109I RGGNCCY 2 cut(s) 404, 972
EcoRI GAATTC 1 cut(s) 329
EcoRII CCWGG 1 cut(s) 260
EcoRV GATATC 1 cut(s) 631
EcoT14I CCWWGG 3 cut(s) 174, 697, 870
EcoT38I GRGCYC 3 cut(s) 213, 225, 666
ErhI CCWWGG 3 cut(s) 174, 697, 870
FaeI CATG 3 cut(s) 644, 662, 971
FalI AAGNNNNNCTT 2 cut(s) 494, 526
FaqI GGGAC 1 cut(s) 985
FatI CATG 3 cut(s) 640, 658, 967
FauI CCCGC 2 cut(s) 923, 983
FauNDI CATATG 1 cut(s) 469
Fnu4HI GCNGC 3 cut(s) 143, 189, 965
FokI GGATG 1 cut(s) 887
FriOI GRGCYC 3 cut(s) 213, 225, 666
Fsp4HI GCNGC 3 cut(s) 143, 189, 965
FspBI CTAG 2 cut(s) 399, 1029
GlaI GCGC 4 cut(s) 164, 200, 202, 350
GluI GCNGC 3 cut(s) 143, 189, 965
GsaI CCCAGC 1 cut(s) 585
HaeIII GGCC 5 cut(s) 173, 179, 406, 586, 933
HapII CCGG 4 cut(s) 65, 227, 805, 1016
HgaI GACGC 1 cut(s) 194
HhaI GCGC 4 cut(s) 165, 201, 203, 351
Hin1II CATG 3 cut(s) 644, 662, 971
Hin6I GCGC 4 cut(s) 163, 199, 201, 349
HinP1I GCGC 4 cut(s) 163, 199, 201, 349
HindIII AAGCTT 2 cut(s) 152, 611
HinfI GANTC 5 cut(s) 82, 353, 358, 637, 851
HpaII CCGG 4 cut(s) 65, 227, 805, 1016
HphI GGTGA 2 cut(s) 367, 934
Hpy166II GTNNAC 3 cut(s) 127, 532, 1009
Hpy188III TCNNGA 4 cut(s) 206, 241, 634, 659
Hpy8I GTNNAC 3 cut(s) 127, 532, 1009
Hpy99I CGWCG 3 cut(s) 44, 94, 296
HpyAV CCTTC 3 cut(s) 163, 985, 1029
HpyCH4III ACNGT 2 cut(s) 280, 726
HpyCH4V TGCA 4 cut(s) 446, 532, 878, 964
HpyF10VI GCNNNNNNNGC 6 cut(s) 128, 142, 151, 160, 185, 610
HpyF3I CTNAG 3 cut(s) 192, 408, 885
Hsp92II CATG 3 cut(s) 644, 662, 971
HspAI GCGC 4 cut(s) 163, 199, 201, 349
Kzo9I GATC 2 cut(s) 237, 435
LguI GCTCTTC 1 cut(s) 294
LmnI GCTCC 1 cut(s) 220
Lsp1109I GCAGC 3 cut(s) 154, 175, 976
LweI GCATC 4 cut(s) 310, 544, 817, 865
MaeI CTAG 2 cut(s) 399, 1029
MaeIII GTNAC 4 cut(s) 14, 182, 726, 769
MalI GATC 2 cut(s) 239, 437
MbiI CCGCTC 1 cut(s) 891
MboI GATC 2 cut(s) 237, 435
MflI RGATCY 1 cut(s) 237
MhlI GDGCHC 4 cut(s) 213, 225, 534, 666
MluCI AATT 6 cut(s) 329, 391, 522, 567, 909, 954
MlyI GAGTC 1 cut(s) 860
MmeI TCCRAC 1 cut(s) 674
MnlI CCTC 8 cut(s) 15, 38, 41, 63, 108, 211, 455, 945
MroXI GAANNNNTTC 1 cut(s) 509
MseI TTAA 5 cut(s) 344, 371, 677, 719, 948
MspI CCGG 4 cut(s) 65, 227, 805, 1016
MspR9I CCNGG 2 cut(s) 227, 262
MvaI CCWGG 1 cut(s) 262
MvnI CGCG 2 cut(s) 201, 351
MwoI GCNNNNNNNGC 6 cut(s) 128, 142, 151, 160, 185, 610
NciI CCSGG 1 cut(s) 227
NdeI CATATG 1 cut(s) 469
NdeII GATC 2 cut(s) 237, 435
NlaIII CATG 3 cut(s) 644, 662, 971
NlaIV GGNNCC 4 cut(s) 222, 405, 549, 973
NmeAIII GCCGAG 1 cut(s) 192
NmuCI GTSAC 2 cut(s) 182, 726
PaeR7I CTCGAG 1 cut(s) 206
PagI TCATGA 1 cut(s) 658
PauI GCGCGC 1 cut(s) 199
PciSI GCTCTTC 1 cut(s) 294
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PdmI GAANNNNTTC 1 cut(s) 509
PfeI GAWTC 4 cut(s) 82, 353, 358, 637
PkrI GCNGC 3 cut(s) 144, 190, 966
PleI GAGTC 1 cut(s) 859
PpsI GAGTC 1 cut(s) 859
PpuMI RGGWCCY 1 cut(s) 972
PshBI ATTAAT 2 cut(s) 371, 719
PsiI TTATAA 1 cut(s) 416
Psp124BI GAGCTC 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 972
Psp6I CCWGG 1 cut(s) 260
PspFI CCCAGC 1 cut(s) 581
PspGI CCWGG 1 cut(s) 260
PspN4I GGNNCC 4 cut(s) 222, 405, 549, 973
PspPI GGNCC 7 cut(s) 124, 178, 404, 584, 893, 932, 972
PspPPI RGGWCCY 1 cut(s) 972
PstI CTGCAG 1 cut(s) 966
PsuI RGATCY 1 cut(s) 237
PteI GCGCGC 1 cut(s) 199
RsaI GTAC 1 cut(s) 494
RsaNI GTAC 1 cut(s) 493
SacI GAGCTC 1 cut(s) 213
SapI GCTCTTC 1 cut(s) 294
SaqAI TTAA 5 cut(s) 344, 371, 677, 719, 948
SatI GCNGC 3 cut(s) 143, 189, 965
Sau3AI GATC 2 cut(s) 237, 435
Sau96I GGNCC 7 cut(s) 124, 178, 404, 584, 893, 932, 972
SchI GAGTC 1 cut(s) 860
ScrFI CCNGG 2 cut(s) 227, 262
SduI GDGCHC 4 cut(s) 213, 225, 534, 666
SfaNI GCATC 4 cut(s) 310, 544, 817, 865
SfcI CTRYAG 2 cut(s) 920, 962
Sfr274I CTCGAG 1 cut(s) 206
SinI GGWCC 3 cut(s) 124, 893, 972
SlaI CTCGAG 1 cut(s) 206
SmlI CTYRAG 3 cut(s) 137, 155, 206
SmoI CTYRAG 3 cut(s) 137, 155, 206
Sse9I AATT 6 cut(s) 329, 391, 522, 567, 909, 954
SsiI CCGC 5 cut(s) 62, 122, 891, 916, 990
SspMI CTAG 2 cut(s) 399, 1029
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 2 cut(s) 225, 260
StyI CCWWGG 3 cut(s) 174, 697, 870
TaaI ACNGT 2 cut(s) 280, 726
TaqI TCGA 6 cut(s) 92, 207, 270, 338, 356, 978
TasI AATT 6 cut(s) 329, 391, 522, 567, 909, 954
TfiI GAWTC 4 cut(s) 82, 353, 358, 637
Tru1I TTAA 5 cut(s) 344, 371, 677, 719, 948
Tru9I TTAA 5 cut(s) 344, 371, 677, 719, 948
TseFI GTSAC 2 cut(s) 182, 726
TseI GCWGC 3 cut(s) 142, 188, 964
Tsp45I GTSAC 2 cut(s) 182, 726
TspDTI ATGAA 2 cut(s) 312, 342
TspGWI ACGGA 3 cut(s) 57, 421, 743
VneI GTGCAC 1 cut(s) 530
VpaK11BI GGWCC 3 cut(s) 124, 893, 972
VspI ATTAAT 2 cut(s) 371, 719
XapI RAATTY 1 cut(s) 329
XhoI CTCGAG 1 cut(s) 206
XmnI GAANNNNTTC 1 cut(s) 509
XspI CTAG 2 cut(s) 399, 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.