Rw6G003520

Ribosome production factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002361
Physical Location & Seq
Reverse (-)
5741371 .. 5745484
4114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G003520.1

Sequence Viewer

Length: 981 bp
ATGAACTCAGTGCGGTTTGCTCTTGTCATTTCACTAGATAACTCGACCAAAGCGGCCAATGACAAGAAAAAGACAAAGAAACTGAGGAAAGGAAAAGGAAAGAAGGTGAAGAACAATAAGGATTTGGATGATCCCTCTAATACCGATGAGAGTTTGCAGTTGTTTGTTATAAATGATGCGGATGAGTTTAGTGCGGTTTTGAAGCGTGAATCGAATCCGAAGATATTGATCACCACTTGTCGATTTAACTCTTCTAGGGGTCCTGCTTTTATAGAGGAACTACTTTCGGTGATCCCAAATGCGCAGTACTATAAAAGAGACACTTATGACTTGAAAAAGATTATAGAGTATGCAAATACAAAGGAATTCACTTCTTTAATTGTTGTTCATACCAATCACCGGGAACCAGATGCTCTCCTAATTATTGGCTTACCTAATGGACCTACTGCCCATTTTAAGCTCTCAAAGCTTGTTTTACGGAAGGATATCAAGAATCATGGAAATCCAACTAGTCATGAGCTTGAGCTTGTATTAAACAACTTTACAACATGCCTTGGTCATCGTATTGGGAGTACTTTTCCTCAAGAACCAAATTTTAGTGGTCGGCGAGTTGTAACTTTCCACAACCACGAGATTTTATATTCTTCCGGCATCATCGGTGTGCCTCATTTGCTATTTTTAGTTCTTGCAGTGATCCTTTCTTATACTTGTGATCTTATATACTTTCCTTTGCTGTACATTTTTGAAACCAAAGAGACTAAACAGGCTGAAGTAAAGGGCAAAAAGACCAAGGATTCCAAAGCTGACGCTATTACTGAAGCAAAACCATTTGCGCGCCTACAGGAATGTGGTCCTCATTTTACCCTTAAATTGATCAGTCTGCAACACGGGACATTTGATCCTAGAGGCGGGGAGTTTGAGTGGGTTCACAAGACGACGGTGAGTCTTCAAATCAGACGACGTTTTTTCACCGTCGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Brix

Protein Analysis

326

Amino Acids

37.01

Weight (kDa)

9.46

Isoelectric Point (pI)

34.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Brix PF04427 83 - 292 1.6e-22 Brix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 170
Acc16I TGCGCA 1 cut(s) 303
AccII CGCG 1 cut(s) 835
AciI CCGC 5 cut(s) 13, 53, 179, 194, 909
AclWI GGATC 4 cut(s) 125, 286, 688, 893
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 2 cut(s) 365, 592
AcuI CTGAAG 2 cut(s) 789, 837
AfaI GTAC 3 cut(s) 308, 574, 737
AfiI CCNNNNNNNGG 2 cut(s) 399, 908
AgsI TTSAA 4 cut(s) 202, 334, 746, 950
AhdI GACNNNNNGTC 1 cut(s) 942
AhlI ACTAGT 1 cut(s) 509
AleI CACNNNNGTG 1 cut(s) 974
AluBI AGCT 5 cut(s) 460, 469, 520, 526, 803
AluI AGCT 5 cut(s) 460, 469, 520, 526, 803
Alw26I GTCTC 2 cut(s) 312, 749
AlwI GGATC 4 cut(s) 125, 286, 688, 893
AoxI GGCC 1 cut(s) 54
ApoI RAATTY 2 cut(s) 365, 592
AspLEI GCGC 3 cut(s) 304, 835, 837
AspS9I GGNCC 3 cut(s) 260, 440, 851
AsuC2I CCSGG 1 cut(s) 401
AsuHPI GGTGA 6 cut(s) 118, 223, 301, 389, 952, 961
AvaII GGWCC 3 cut(s) 260, 440, 851
BauI CACGAG 1 cut(s) 629
BbsI GAAGAC 1 cut(s) 938
BclI TGATCA 2 cut(s) 228, 873
BcnI CCSGG 1 cut(s) 401
BcoDI GTCTC 2 cut(s) 312, 749
BcuI ACTAGT 1 cut(s) 509
BfaI CTAG 4 cut(s) 35, 255, 510, 903
BfmI CTRYAG 1 cut(s) 839
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
BmcAI AGTACT 2 cut(s) 308, 574
Bme1390I CCNGG 1 cut(s) 401
Bme18I GGWCC 3 cut(s) 260, 440, 851
BmeRI GACNNNNNGTC 1 cut(s) 942
BmgT120I GGNCC 3 cut(s) 260, 440, 851
BmiI GGNNCC 2 cut(s) 261, 405
BmrFI CCNGG 1 cut(s) 401
BmsI GCATC 3 cut(s) 166, 400, 660
BpiI GAAGAC 1 cut(s) 938
BpuEI CTTGAG 2 cut(s) 542, 567
BpuMI CCSGG 1 cut(s) 401
BsaBI GATNNNNATC 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 553, 789
BsaXI ACNNNNNCTCC 2 cut(s) 905, 935
Bsc4I CCNNNNNNNGG 2 cut(s) 399, 908
Bse8I GATNNNNATC 1 cut(s) 227
BseDI CCNNGG 2 cut(s) 553, 789
BseGI GGATG 2 cut(s) 133, 187
BseJI GATNNNNATC 1 cut(s) 227
BseLI CCNNNNNNNGG 2 cut(s) 399, 908
BseMII CTCAG 2 cut(s) 21, 74
BsePI GCGCGC 1 cut(s) 833
Bsh1236I CGCG 1 cut(s) 835
BshFI GGCC 1 cut(s) 56
BsiSI CCGG 2 cut(s) 400, 648
BslFI GGGAC 1 cut(s) 904
BslI CCNNNNNNNGG 2 cut(s) 399, 908
BsmAI GTCTC 2 cut(s) 312, 749
BsmFI GGGAC 1 cut(s) 904
BsnI GGCC 1 cut(s) 56
Bsp1407I TGTACA 1 cut(s) 735
Bsp143I GATC 7 cut(s) 130, 228, 291, 693, 712, 873, 898
BspACI CCGC 5 cut(s) 13, 53, 179, 194, 909
BspANI GGCC 1 cut(s) 56
BspCNI CTCAG 2 cut(s) 20, 75
BspFNI CGCG 1 cut(s) 835
BspHI TCATGA 1 cut(s) 514
BspLI GGNNCC 2 cut(s) 261, 405
BspPI GGATC 4 cut(s) 125, 286, 688, 893
BsrGI TGTACA 1 cut(s) 735
BssECI CCNNGG 2 cut(s) 553, 789
BssHII GCGCGC 1 cut(s) 833
BssMI GATC 7 cut(s) 130, 228, 291, 693, 712, 873, 898
BssSI CACGAG 1 cut(s) 629
BssT1I CCWWGG 2 cut(s) 553, 789
Bst2BI CACGAG 1 cut(s) 629
Bst4CI ACNGT 2 cut(s) 940, 973
Bst6I CTCTTC 1 cut(s) 256
BstAUI TGTACA 1 cut(s) 735
BstC8I GCNNGC 1 cut(s) 835
BstDEI CTNAG 2 cut(s) 7, 83
BstF5I GGATG 2 cut(s) 133, 187
BstFNI CGCG 1 cut(s) 835
BstHHI GCGC 3 cut(s) 304, 835, 837
BstKTI GATC 7 cut(s) 133, 231, 294, 696, 715, 876, 901
BstMAI GTCTC 2 cut(s) 312, 749
BstMBI GATC 7 cut(s) 130, 228, 291, 693, 712, 873, 898
BstMWI GCNNNNNNNGC 2 cut(s) 466, 670
BstNSI RCATGY 1 cut(s) 552
BstSCI CCNGG 1 cut(s) 399
BstSFI CTRYAG 1 cut(s) 839
BstUI CGCG 1 cut(s) 835
BstV2I GAAGAC 1 cut(s) 938
BsuRI GGCC 1 cut(s) 56
BtsCI GGATG 2 cut(s) 133, 187
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 2 cut(s) 15, 696
Cac8I GCNNGC 1 cut(s) 835
CciI TCATGA 1 cut(s) 514
CfoI GCGC 3 cut(s) 304, 835, 837
Cfr13I GGNCC 3 cut(s) 260, 440, 851
CseI GACGC 1 cut(s) 815
Csp6I GTAC 3 cut(s) 307, 573, 736
CviAII CATG 3 cut(s) 497, 515, 549
CviJI RGCY 8 cut(s) 56, 429, 460, 469, 520, 526, 767, 803
CviKI_1 RGCY 8 cut(s) 56, 429, 460, 469, 520, 526, 767, 803
CviQI GTAC 3 cut(s) 307, 573, 736
DdeI CTNAG 2 cut(s) 7, 83
DpnI GATC 7 cut(s) 132, 230, 293, 695, 714, 875, 900
DpnII GATC 7 cut(s) 130, 228, 291, 693, 712, 873, 898
DriI GACNNNNNGTC 1 cut(s) 942
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 256
Eam1105I GACNNNNNGTC 1 cut(s) 942
EarI CTCTTC 1 cut(s) 256
Eco130I CCWWGG 2 cut(s) 553, 789
Eco32I GATATC 1 cut(s) 487
Eco47I GGWCC 3 cut(s) 260, 440, 851
Eco57I CTGAAG 2 cut(s) 789, 837
EcoO109I RGGNCCY 1 cut(s) 260
EcoRI GAATTC 1 cut(s) 365
EcoRV GATATC 1 cut(s) 487
EcoT14I CCWWGG 2 cut(s) 553, 789
ErhI CCWWGG 2 cut(s) 553, 789
FaeI CATG 3 cut(s) 500, 518, 552
FalI AAGNNNNNCTT 2 cut(s) 307, 339
FaqI GGGAC 1 cut(s) 904
FatI CATG 3 cut(s) 496, 514, 548
FauI CCCGC 1 cut(s) 902
FbaI TGATCA 2 cut(s) 228, 873
Fnu4HI GCNGC 1 cut(s) 54
FokI GGATG 2 cut(s) 140, 194
Fsp4HI GCNGC 1 cut(s) 54
FspBI CTAG 4 cut(s) 35, 255, 510, 903
FspI TGCGCA 1 cut(s) 303
GlaI GCGC 3 cut(s) 303, 834, 836
GluI GCNGC 1 cut(s) 54
HaeIII GGCC 1 cut(s) 56
HapII CCGG 2 cut(s) 400, 648
HgaI GACGC 1 cut(s) 815
HhaI GCGC 3 cut(s) 304, 835, 837
Hin1II CATG 3 cut(s) 500, 518, 552
Hin6I GCGC 3 cut(s) 302, 833, 835
HinP1I GCGC 3 cut(s) 302, 833, 835
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 5 cut(s) 209, 214, 493, 794, 943
HpaII CCGG 2 cut(s) 400, 648
HphI GGTGA 6 cut(s) 118, 223, 301, 389, 952, 961
Hpy166II GTNNAC 1 cut(s) 928
Hpy188I TCNGA 2 cut(s) 219, 956
Hpy188III TCNNGA 3 cut(s) 490, 515, 584
Hpy8I GTNNAC 1 cut(s) 928
Hpy99I CGWCG 3 cut(s) 940, 963, 977
HpyAV CCTTC 2 cut(s) 97, 475
HpyCH4III ACNGT 2 cut(s) 940, 973
HpyCH4IV ACGT 1 cut(s) 961
HpyCH4V TGCA 4 cut(s) 157, 353, 689, 883
HpyF10VI GCNNNNNNNGC 2 cut(s) 466, 670
HpyF3I CTNAG 2 cut(s) 7, 83
HpySE526I ACGT 1 cut(s) 961
Hsp92II CATG 3 cut(s) 500, 518, 552
HspAI GCGC 3 cut(s) 302, 833, 835
Ksp22I TGATCA 2 cut(s) 228, 873
Kzo9I GATC 7 cut(s) 130, 228, 291, 693, 712, 873, 898
LpnPI CCDG 6 cut(s) 276, 413, 420, 661, 749, 827
LweI GCATC 3 cut(s) 166, 400, 660
MaeI CTAG 4 cut(s) 35, 255, 510, 903
MaeII ACGT 1 cut(s) 961
MaeIII GTNAC 1 cut(s) 613
MalI GATC 7 cut(s) 132, 230, 293, 695, 714, 875, 900
MboI GATC 7 cut(s) 130, 228, 291, 693, 712, 873, 898
MboII GAAGA 5 cut(s) 121, 232, 243, 636, 938
MluCI AATT 5 cut(s) 365, 378, 420, 592, 869
MlyI GAGTC 1 cut(s) 952
MmeI TCCRAC 1 cut(s) 530
MnlI CCTC 7 cut(s) 78, 145, 268, 591, 675, 864, 899
MseI TTAA 5 cut(s) 246, 377, 456, 533, 867
MslI CAYNNNNRTG 2 cut(s) 659, 974
MspI CCGG 2 cut(s) 400, 648
MspR9I CCNGG 1 cut(s) 401
MvnI CGCG 1 cut(s) 835
MwoI GCNNNNNNNGC 2 cut(s) 466, 670
NciI CCSGG 1 cut(s) 401
NdeII GATC 7 cut(s) 130, 228, 291, 693, 712, 873, 898
NlaIII CATG 3 cut(s) 500, 518, 552
NlaIV GGNNCC 2 cut(s) 261, 405
NsbI TGCGCA 1 cut(s) 303
NspI RCATGY 1 cut(s) 552
OliI CACNNNNGTG 1 cut(s) 974
PagI TCATGA 1 cut(s) 514
PauI GCGCGC 1 cut(s) 833
PfeI GAWTC 4 cut(s) 209, 214, 493, 794
PkrI GCNGC 1 cut(s) 55
PleI GAGTC 1 cut(s) 951
PpsI GAGTC 1 cut(s) 951
PpuMI RGGWCCY 1 cut(s) 260
PsiI TTATAA 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 260
PspN4I GGNNCC 2 cut(s) 261, 405
PspPI GGNCC 3 cut(s) 260, 440, 851
PspPPI RGGWCCY 1 cut(s) 260
PteI GCGCGC 1 cut(s) 833
RsaI GTAC 3 cut(s) 308, 574, 737
RsaNI GTAC 3 cut(s) 307, 573, 736
RseI CAYNNNNRTG 2 cut(s) 659, 974
SaqAI TTAA 5 cut(s) 246, 377, 456, 533, 867
SatI GCNGC 1 cut(s) 54
Sau3AI GATC 7 cut(s) 130, 228, 291, 693, 712, 873, 898
Sau96I GGNCC 3 cut(s) 260, 440, 851
ScaI AGTACT 2 cut(s) 308, 574
SchI GAGTC 1 cut(s) 952
ScrFI CCNGG 1 cut(s) 401
SetI ASST 9 cut(s) 108, 436, 445, 462, 471, 522, 528, 805, 964
SfaNI GCATC 3 cut(s) 166, 400, 660
SfcI CTRYAG 1 cut(s) 839
SinI GGWCC 3 cut(s) 260, 440, 851
SmiMI CAYNNNNRTG 2 cut(s) 659, 974
SmlI CTYRAG 2 cut(s) 521, 582
SmoI CTYRAG 2 cut(s) 521, 582
SpeI ACTAGT 1 cut(s) 509
Sse9I AATT 5 cut(s) 365, 378, 420, 592, 869
SsiI CCGC 5 cut(s) 13, 53, 179, 194, 909
SspMI CTAG 4 cut(s) 35, 255, 510, 903
StyD4I CCNGG 1 cut(s) 399
StyI CCWWGG 2 cut(s) 553, 789
TaaI ACNGT 2 cut(s) 940, 973
TaiI ACGT 1 cut(s) 964
TaqI TCGA 3 cut(s) 44, 212, 241
TasI AATT 5 cut(s) 365, 378, 420, 592, 869
TatI WGTACW 3 cut(s) 306, 572, 735
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 4 cut(s) 209, 214, 493, 794
Tru1I TTAA 5 cut(s) 246, 377, 456, 533, 867
Tru9I TTAA 5 cut(s) 246, 377, 456, 533, 867
TscAI CASTG 2 cut(s) 15, 696
TspDTI ATGAA 2 cut(s) 17, 377
TspGWI ACGGA 1 cut(s) 493
TspRI CASTG 2 cut(s) 15, 696
VpaK11BI GGWCC 3 cut(s) 260, 440, 851
XapI RAATTY 2 cut(s) 365, 592
XceI RCATGY 1 cut(s) 552
XspI CTAG 4 cut(s) 35, 255, 510, 903
ZrmI AGTACT 2 cut(s) 308, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.