RLG00000027705

Ribosome production factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002361
Physical Location & Seq
Forward (+)
14179365 .. 14181985
2621 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027705

Sequence Viewer

Length: 1059 bp
ATGGGCTTCAAGAGGAAGAAGAGCGAGAACGAAGGAGGAGACGAGCCCAAACCAGTAGCAGTGGAGATTGAGAAGAGGAAGAAGGGGATACTGCCGTCGATGATAAAGAACAAGGACAAGAGAGCTGCCGTCCACGCCAAGCTCAAGCAAGAGAAGAAGCTCGATAAGCGCAAGAAGGTCAAGGCCCGCGACGCCGCCGAAAAGCGAGCTCTCGAGCTCGGCGAAGAGCCTCCGGCGAGGAAGATTCCGAAGACGATTGAGAACACCAGGGAGGAGGATGAGACTGTTTGTAGGCCCGACGACGAAGAGTTGTTTGTTAGAAATGATGCGGATGAGTTTAGTGCGGTTTTGAAGCGTGAATCGAATCCGAAGATATTGATCACCACTTGCCGATTTAACTCTTCTAGGGGTCCTGCTTTTATAGAGGAACTACTTTCGGTGATCCCAAATGCACAGTACTATAAAAGAGGCACTTATGACTTGAAAAAGATTATAGAGTATGCAAATAAAAAGGAATTCACTTCTTTAATTGTTGTTCATACCAATCGCCGGGAACCAGATGCTCTCCTAATTATTGGCTTACCTAATGGACCTACTGCCCATTTTAAGCTCTCAAAGCTTGTTTTACGGAAGGATATCAAGAATCATGGAAATCCAACCAGTCATGAGCCTGAGCTTGTATTAAACAACTTTACAACACGCCTTGGTCATCGTATTGGGAGATTAATACAGTCACTTTTCCCTCAAGAACCAAATTTTCGTGGTCGGCGAGTTGTAACTTTCCACAACCAGCGAGATTTTATATTCTTCCGGCATCATCGGTACATTTTTGAAACCAAAGAGACTAAACAGGCTGAAGTAAAGGGCAAAAAGACCAAGGATTCCAAAGCTGACTCTATTACTGAACCGAAACCATTTGCGCGCCTACAGGAATGTGGTCCTCGTTTTACCCTTAAATTGATCAGTCTGCAGCATGGGACATTTGATCCTAGAGGCGGGGAGTTTGAGTGGGTTCACAAGCCTGAGATGGACACTAGCAGAAGGAGGTTTTTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Brix

Protein Analysis

353

Amino Acids

40.87

Weight (kDa)

9.84

Isoelectric Point (pI)

47.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Brix PF04427 133 - 321 6.4e-34 Brix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 189, 922
AciI CCGC 5 cut(s) 187, 195, 329, 344, 996
AclWI GGATC 2 cut(s) 436, 980
AcsI RAATTY 2 cut(s) 515, 754
AcuI CTGAAG 1 cut(s) 876
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 2 cut(s) 458, 824
AfiI CCNNNNNNNGG 2 cut(s) 549, 995
AgsI TTSAA 4 cut(s) 10, 352, 484, 833
AjnI CCWGG 1 cut(s) 266
AluBI AGCT 9 cut(s) 125, 142, 160, 209, 217, 610, 619, 676, 890
AluI AGCT 9 cut(s) 125, 142, 160, 209, 217, 610, 619, 676, 890
Alw21I GWGCWC 2 cut(s) 211, 219
Alw26I GTCTC 3 cut(s) 33, 275, 836
AlwI GGATC 2 cut(s) 436, 980
Ama87I CYCGRG 1 cut(s) 212
AoxI GGCC 2 cut(s) 183, 293
ApeKI GCWGC 2 cut(s) 125, 970
ApoI RAATTY 2 cut(s) 515, 754
AseI ATTAAT 1 cut(s) 725
AspLEI GCGC 3 cut(s) 171, 922, 924
AspS9I GGNCC 5 cut(s) 184, 294, 410, 590, 938
AsuC2I CCSGG 1 cut(s) 551
AsuHPI GGTGA 2 cut(s) 373, 451
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 3 cut(s) 410, 590, 938
BaeI ACNNNNGTAYC 2 cut(s) 80, 113
BanII GRGCYC 3 cut(s) 48, 211, 219
BbsI GAAGAC 1 cut(s) 257
Bbv12I GWGCWC 2 cut(s) 211, 219
BbvI GCAGC 2 cut(s) 112, 982
BccI CCATC 1 cut(s) 1021
BceAI ACGGC 2 cut(s) 79, 113
BciT130I CCWGG 1 cut(s) 268
BciVI GTATCC 1 cut(s) 81
BclI TGATCA 2 cut(s) 378, 960
BcnI CCSGG 1 cut(s) 551
BcoDI GTCTC 3 cut(s) 33, 275, 836
BfaI CTAG 3 cut(s) 405, 990, 1035
BfmI CTRYAG 2 cut(s) 926, 968
BfuI GTATCC 1 cut(s) 81
BisI GCNGC 3 cut(s) 126, 195, 971
BlsI GCNGC 3 cut(s) 127, 196, 972
BmcAI AGTACT 1 cut(s) 458
Bme1390I CCNGG 2 cut(s) 268, 551
Bme18I GGWCC 3 cut(s) 410, 590, 938
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 5 cut(s) 184, 294, 410, 590, 938
BmiI GGNNCC 2 cut(s) 411, 555
BmrFI CCNGG 2 cut(s) 268, 551
BmsI GCATC 3 cut(s) 316, 550, 823
BpiI GAAGAC 1 cut(s) 257
Bpu10I CCTNAGC 1 cut(s) 672
BpuEI CTTGAG 2 cut(s) 128, 729
BpuMI CCSGG 1 cut(s) 551
BsaBI GATNNNNATC 1 cut(s) 377
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 3 cut(s) 267, 703, 876
BsaXI ACNNNNNCTCC 2 cut(s) 992, 1022
Bsc4I CCNNNNNNNGG 2 cut(s) 549, 995
Bse1I ACTGG 2 cut(s) 53, 660
Bse8I GATNNNNATC 1 cut(s) 377
BseBI CCWGG 1 cut(s) 268
BseDI CCNNGG 3 cut(s) 267, 703, 876
BseGI GGATG 2 cut(s) 283, 337
BseJI GATNNNNATC 1 cut(s) 377
BseLI CCNNNNNNNGG 2 cut(s) 549, 995
BseMII CTCAG 2 cut(s) 663, 1014
BseNI ACTGG 2 cut(s) 53, 660
BsePI GCGCGC 1 cut(s) 920
BseRI GAGGAG 2 cut(s) 51, 287
BseXI GCAGC 2 cut(s) 112, 982
Bsh1236I CGCG 2 cut(s) 189, 922
BshFI GGCC 2 cut(s) 185, 295
BsiHKAI GWGCWC 2 cut(s) 211, 219
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 3 cut(s) 233, 550, 811
BslFI GGGAC 1 cut(s) 991
BslI CCNNNNNNNGG 2 cut(s) 549, 995
BsmAI GTCTC 3 cut(s) 33, 275, 836
BsmBI CGTCTC 1 cut(s) 33
BsmFI GGGAC 1 cut(s) 991
BsnI GGCC 2 cut(s) 185, 295
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 3 cut(s) 48, 211, 219
Bsp143I GATC 4 cut(s) 378, 441, 960, 985
BspACI CCGC 5 cut(s) 187, 195, 329, 344, 996
BspANI GGCC 2 cut(s) 185, 295
BspCNI CTCAG 2 cut(s) 664, 1015
BspFNI CGCG 2 cut(s) 189, 922
BspHI TCATGA 1 cut(s) 664
BspLI GGNNCC 2 cut(s) 411, 555
BspMAI CTGCAG 1 cut(s) 972
BspPI GGATC 2 cut(s) 436, 980
BspQI GCTCTTC 2 cut(s) 14, 219
BsrI ACTGG 2 cut(s) 53, 660
BssECI CCNNGG 3 cut(s) 267, 703, 876
BssHII GCGCGC 1 cut(s) 920
BssMI GATC 4 cut(s) 378, 441, 960, 985
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 2 cut(s) 703, 876
Bst2UI CCWGG 1 cut(s) 268
Bst4CI ACNGT 3 cut(s) 286, 456, 732
Bst6I CTCTTC 5 cut(s) 14, 68, 219, 300, 406
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 3 cut(s) 187, 207, 922
BstDEI CTNAG 2 cut(s) 672, 1023
BstF5I GGATG 2 cut(s) 283, 337
BstFNI CGCG 2 cut(s) 189, 922
BstHHI GCGC 3 cut(s) 171, 922, 924
BstKTI GATC 4 cut(s) 381, 444, 963, 988
BstMAI GTCTC 3 cut(s) 33, 275, 836
BstMBI GATC 4 cut(s) 378, 441, 960, 985
BstMWI GCNNNNNNNGC 4 cut(s) 134, 166, 191, 616
BstNI CCWGG 1 cut(s) 268
BstSCI CCNGG 2 cut(s) 266, 549
BstSFI CTRYAG 2 cut(s) 926, 968
BstUI CGCG 2 cut(s) 189, 922
BstV1I GCAGC 2 cut(s) 112, 982
BstV2I GAAGAC 1 cut(s) 257
BsuI GTATCC 1 cut(s) 81
BsuRI GGCC 2 cut(s) 185, 295
BtsCI GGATG 2 cut(s) 283, 337
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 1 cut(s) 66
Cac8I GCNNGC 3 cut(s) 187, 207, 922
CciI TCATGA 1 cut(s) 664
CfoI GCGC 3 cut(s) 171, 922, 924
Cfr13I GGNCC 5 cut(s) 184, 294, 410, 590, 938
CseI GACGC 1 cut(s) 200
Csp6I GTAC 2 cut(s) 457, 823
CviAII CATG 3 cut(s) 647, 665, 974
CviQI GTAC 2 cut(s) 457, 823
DdeI CTNAG 2 cut(s) 672, 1023
DpnI GATC 4 cut(s) 380, 443, 962, 987
DpnII GATC 4 cut(s) 378, 441, 960, 985
Eam1104I CTCTTC 5 cut(s) 14, 68, 219, 300, 406
EarI CTCTTC 5 cut(s) 14, 68, 219, 300, 406
Ecl136II GAGCTC 2 cut(s) 209, 217
Eco130I CCWWGG 2 cut(s) 703, 876
Eco24I GRGCYC 3 cut(s) 48, 211, 219
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 3 cut(s) 410, 590, 938
Eco53kI GAGCTC 2 cut(s) 209, 217
Eco57I CTGAAG 1 cut(s) 876
Eco88I CYCGRG 1 cut(s) 212
EcoICRI GAGCTC 2 cut(s) 209, 217
EcoO109I RGGNCCY 1 cut(s) 410
EcoRI GAATTC 1 cut(s) 515
EcoRII CCWGG 1 cut(s) 266
EcoRV GATATC 1 cut(s) 637
EcoT14I CCWWGG 2 cut(s) 703, 876
EcoT38I GRGCYC 3 cut(s) 48, 211, 219
ErhI CCWWGG 2 cut(s) 703, 876
Esp3I CGTCTC 1 cut(s) 33
FaeI CATG 3 cut(s) 650, 668, 977
FalI AAGNNNNNCTT 2 cut(s) 457, 489
FaqI GGGAC 1 cut(s) 991
FatI CATG 3 cut(s) 646, 664, 973
FauI CCCGC 2 cut(s) 194, 989
FbaI TGATCA 2 cut(s) 378, 960
Fnu4HI GCNGC 3 cut(s) 126, 195, 971
FokI GGATG 2 cut(s) 290, 344
FriOI GRGCYC 3 cut(s) 48, 211, 219
Fsp4HI GCNGC 3 cut(s) 126, 195, 971
FspBI CTAG 3 cut(s) 405, 990, 1035
GlaI GCGC 3 cut(s) 170, 921, 923
GluI GCNGC 3 cut(s) 126, 195, 971
HaeIII GGCC 2 cut(s) 185, 295
HapII CCGG 3 cut(s) 233, 550, 811
HgaI GACGC 1 cut(s) 200
HhaI GCGC 3 cut(s) 171, 922, 924
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 3 cut(s) 650, 668, 977
Hin6I GCGC 3 cut(s) 169, 920, 922
HinP1I GCGC 3 cut(s) 169, 920, 922
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 6 cut(s) 244, 359, 364, 643, 881, 893
HpaII CCGG 3 cut(s) 233, 550, 811
HphI GGTGA 2 cut(s) 373, 451
Hpy166II GTNNAC 2 cut(s) 133, 1015
Hpy188I TCNGA 2 cut(s) 249, 369
Hpy188III TCNNGA 5 cut(s) 10, 212, 640, 665, 746
Hpy8I GTNNAC 2 cut(s) 133, 1015
Hpy99I CGWCG 4 cut(s) 100, 194, 302, 305
HpyAV CCTTC 5 cut(s) 26, 76, 169, 625, 1035
HpyCH4III ACNGT 3 cut(s) 286, 456, 732
HpyCH4V TGCA 3 cut(s) 452, 503, 970
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 166, 191, 616
HpyF3I CTNAG 2 cut(s) 672, 1023
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 3 cut(s) 650, 668, 977
HspAI GCGC 3 cut(s) 169, 920, 922
Ksp22I TGATCA 2 cut(s) 378, 960
Kzo9I GATC 4 cut(s) 378, 441, 960, 985
LguI GCTCTTC 2 cut(s) 14, 219
Lsp1109I GCAGC 2 cut(s) 112, 982
LweI GCATC 3 cut(s) 316, 550, 823
MaeI CTAG 3 cut(s) 405, 990, 1035
MaeIII GTNAC 2 cut(s) 732, 775
MalI GATC 4 cut(s) 380, 443, 962, 987
MboI GATC 4 cut(s) 378, 441, 960, 985
MhlI GDGCHC 3 cut(s) 48, 211, 219
MluCI AATT 5 cut(s) 515, 528, 570, 754, 956
MlyI GAGTC 1 cut(s) 887
MmeI TCCRAC 1 cut(s) 680
MseI TTAA 6 cut(s) 396, 527, 606, 683, 725, 954
MspI CCGG 3 cut(s) 233, 550, 811
MspR9I CCNGG 2 cut(s) 268, 551
MvaI CCWGG 1 cut(s) 268
MvnI CGCG 2 cut(s) 189, 922
MwoI GCNNNNNNNGC 4 cut(s) 134, 166, 191, 616
NciI CCSGG 1 cut(s) 551
NdeII GATC 4 cut(s) 378, 441, 960, 985
NlaIII CATG 3 cut(s) 650, 668, 977
NlaIV GGNNCC 2 cut(s) 411, 555
NmeAIII GCCGAG 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 732
PaeR7I CTCGAG 1 cut(s) 212
PagI TCATGA 1 cut(s) 664
PauI GCGCGC 1 cut(s) 920
PciSI GCTCTTC 2 cut(s) 14, 219
PcsI WCGNNNNNNNCGW 2 cut(s) 219, 766
PfeI GAWTC 5 cut(s) 244, 359, 364, 643, 881
PkrI GCNGC 3 cut(s) 127, 196, 972
PleI GAGTC 1 cut(s) 887
PpsI GAGTC 1 cut(s) 887
PpuMI RGGWCCY 1 cut(s) 410
PshBI ATTAAT 1 cut(s) 725
Psp124BI GAGCTC 2 cut(s) 211, 219
Psp5II RGGWCCY 1 cut(s) 410
Psp6I CCWGG 1 cut(s) 266
PspGI CCWGG 1 cut(s) 266
PspN4I GGNNCC 2 cut(s) 411, 555
PspPI GGNCC 5 cut(s) 184, 294, 410, 590, 938
PspPPI RGGWCCY 1 cut(s) 410
PstI CTGCAG 1 cut(s) 972
PteI GCGCGC 1 cut(s) 920
RsaI GTAC 2 cut(s) 458, 824
RsaNI GTAC 2 cut(s) 457, 823
SacI GAGCTC 2 cut(s) 211, 219
SapI GCTCTTC 2 cut(s) 14, 219
SaqAI TTAA 6 cut(s) 396, 527, 606, 683, 725, 954
SatI GCNGC 3 cut(s) 126, 195, 971
Sau3AI GATC 4 cut(s) 378, 441, 960, 985
Sau96I GGNCC 5 cut(s) 184, 294, 410, 590, 938
ScaI AGTACT 1 cut(s) 458
SchI GAGTC 1 cut(s) 887
ScrFI CCNGG 2 cut(s) 268, 551
SduI GDGCHC 3 cut(s) 48, 211, 219
SfaNI GCATC 3 cut(s) 316, 550, 823
SfcI CTRYAG 2 cut(s) 926, 968
Sfr274I CTCGAG 1 cut(s) 212
SinI GGWCC 3 cut(s) 410, 590, 938
SlaI CTCGAG 1 cut(s) 212
SmlI CTYRAG 3 cut(s) 143, 212, 744
SmoI CTYRAG 3 cut(s) 143, 212, 744
Sse9I AATT 5 cut(s) 515, 528, 570, 754, 956
SsiI CCGC 5 cut(s) 187, 195, 329, 344, 996
SspMI CTAG 3 cut(s) 405, 990, 1035
SstI GAGCTC 2 cut(s) 211, 219
StyD4I CCNGG 2 cut(s) 266, 549
StyI CCWWGG 2 cut(s) 703, 876
TaaI ACNGT 3 cut(s) 286, 456, 732
TaqI TCGA 4 cut(s) 98, 162, 213, 362
TasI AATT 5 cut(s) 515, 528, 570, 754, 956
TatI WGTACW 1 cut(s) 456
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 5 cut(s) 244, 359, 364, 643, 881
Tru1I TTAA 6 cut(s) 396, 527, 606, 683, 725, 954
Tru9I TTAA 6 cut(s) 396, 527, 606, 683, 725, 954
TscAI CASTG 1 cut(s) 66
TseFI GTSAC 1 cut(s) 732
TseI GCWGC 2 cut(s) 125, 970
Tsp45I GTSAC 1 cut(s) 732
TspDTI ATGAA 1 cut(s) 527
TspGWI ACGGA 1 cut(s) 643
TspRI CASTG 1 cut(s) 66
VpaK11BI GGWCC 3 cut(s) 410, 590, 938
VspI ATTAAT 1 cut(s) 725
XapI RAATTY 2 cut(s) 515, 754
XhoI CTCGAG 1 cut(s) 212
XspI CTAG 3 cut(s) 405, 990, 1035
ZrmI AGTACT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.