RchiOBHm_Chr7g0219071

Ribosome production factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002361
Physical Location & Seq
Reverse (-)
37306218 .. 37308188
1971 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19608

Sequence Viewer

Length: 660 bp
ATGCGTGAATCGAATCCGAAGATATTGATCACCACTTGCCGATTTAACTCTTCTAGGGGTCCTGCTTTTATAGAGGAACTACTTTCGGTGATCCCAAATGCGCAGTACTATAAAAGAGGCACTTATGACTTGAAAAAGATTATAGAGTATGCAAATAAAAAGGAATTCACTTCTTTAATTGTTGTTCATACCAATCGCCGGGAACCAGATGCTCTCCTAATTTTTGGCTTACCTAATGGACCTACTGCCCATTTTAAGCTCTCAAAGCTTGTTTTACAGAAGGATATCAAGAATCATGGAAATCCAACCAGTCATGAGCTTGAGCTTGTATTAAACAACTTTACAACACGCCTTGGTCATCGCATTGGGAGGTACATTTTTGAAACCAAAGAGACTAAACAGGCTGAAGTAAAGGGCAAAAAGACCAAGGATTCCAAAGCTGACGCTATTACTGAAGCAAAACCATTTGTGCGCCTACAGATCTTAACGGTCTTTCCTGTCTTAAACACTCAGGAATGTGGTCCTCGTTTTACCCTTAAATTGATCAGTCTGCAGCACGGGACATTTGATCCTAGAGGCGGGGAGTTGGAGTGGGTTCACAAGGTAATCCACTTGTCAGTCAATTCATTTCAGTATATTGGTTTCATATCTTTATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Brix

Protein Analysis

219

Amino Acids

24.99

Weight (kDa)

9.67

Isoelectric Point (pI)

28.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Brix PF04427 16 - 182 1.9e-15 Brix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 102
AciI CCGC 1 cut(s) 579
AclWI GGATC 2 cut(s) 85, 563
AcsI RAATTY 1 cut(s) 164
AcuI CTGAAG 2 cut(s) 426, 474
AfaI GTAC 2 cut(s) 107, 374
AfiI CCNNNNNNNGG 2 cut(s) 198, 578
AgsI TTSAA 2 cut(s) 133, 383
AluBI AGCT 5 cut(s) 259, 268, 319, 325, 440
AluI AGCT 5 cut(s) 259, 268, 319, 325, 440
Alw26I GTCTC 1 cut(s) 386
AlwI GGATC 2 cut(s) 85, 563
ApeKI GCWGC 1 cut(s) 553
ApoI RAATTY 1 cut(s) 164
AspLEI GCGC 2 cut(s) 103, 474
AspS9I GGNCC 3 cut(s) 59, 239, 521
AsuC2I CCSGG 1 cut(s) 200
AsuHPI GGTGA 2 cut(s) 22, 100
AvaII GGWCC 3 cut(s) 59, 239, 521
BbvI GCAGC 1 cut(s) 565
BclI TGATCA 2 cut(s) 27, 543
BcnI CCSGG 1 cut(s) 200
BcoDI GTCTC 1 cut(s) 386
BfaI CTAG 2 cut(s) 54, 573
BfmI CTRYAG 2 cut(s) 476, 551
BglII AGATCT 1 cut(s) 480
BisI GCNGC 1 cut(s) 554
BlsI GCNGC 1 cut(s) 555
BmcAI AGTACT 1 cut(s) 107
Bme1390I CCNGG 1 cut(s) 200
Bme18I GGWCC 3 cut(s) 59, 239, 521
BmgT120I GGNCC 3 cut(s) 59, 239, 521
BmiI GGNNCC 2 cut(s) 60, 204
BmrFI CCNGG 1 cut(s) 200
BmsI GCATC 1 cut(s) 199
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 200
BsaBI GATNNNNATC 1 cut(s) 26
BsaJI CCNNGG 2 cut(s) 352, 426
BsaXI ACNNNNNCTCC 2 cut(s) 575, 605
Bsc4I CCNNNNNNNGG 2 cut(s) 198, 578
Bse1I ACTGG 1 cut(s) 309
Bse8I GATNNNNATC 1 cut(s) 26
BseDI CCNNGG 2 cut(s) 352, 426
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 2 cut(s) 198, 578
BseMII CTCAG 1 cut(s) 524
BseNI ACTGG 1 cut(s) 309
BseXI GCAGC 1 cut(s) 565
BsiSI CCGG 1 cut(s) 199
BslFI GGGAC 1 cut(s) 574
BslI CCNNNNNNNGG 2 cut(s) 198, 578
BsmAI GTCTC 1 cut(s) 386
BsmFI GGGAC 1 cut(s) 574
Bsp143I GATC 5 cut(s) 27, 90, 480, 543, 568
BspACI CCGC 1 cut(s) 579
BspCNI CTCAG 1 cut(s) 523
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 2 cut(s) 60, 204
BspMAI CTGCAG 1 cut(s) 555
BspPI GGATC 2 cut(s) 85, 563
BsrI ACTGG 1 cut(s) 309
BssECI CCNNGG 2 cut(s) 352, 426
BssMI GATC 5 cut(s) 27, 90, 480, 543, 568
BssT1I CCWWGG 2 cut(s) 352, 426
Bst4CI ACNGT 1 cut(s) 490
Bst6I CTCTTC 1 cut(s) 55
BstDEI CTNAG 1 cut(s) 510
BstHHI GCGC 2 cut(s) 103, 474
BstKTI GATC 5 cut(s) 30, 93, 483, 546, 571
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 5 cut(s) 27, 90, 480, 543, 568
BstMWI GCNNNNNNNGC 1 cut(s) 265
BstSCI CCNGG 1 cut(s) 198
BstSFI CTRYAG 2 cut(s) 476, 551
BstV1I GCAGC 1 cut(s) 565
BstX2I RGATCY 1 cut(s) 480
BstYI RGATCY 1 cut(s) 480
BtgZI GCGATG 1 cut(s) 344
CciI TCATGA 1 cut(s) 313
CfoI GCGC 2 cut(s) 103, 474
Cfr13I GGNCC 3 cut(s) 59, 239, 521
CseI GACGC 1 cut(s) 452
Csp6I GTAC 2 cut(s) 106, 373
CviAII CATG 2 cut(s) 296, 314
CviJI RGCY 7 cut(s) 228, 259, 268, 319, 325, 404, 440
CviKI_1 RGCY 7 cut(s) 228, 259, 268, 319, 325, 404, 440
CviQI GTAC 2 cut(s) 106, 373
DdeI CTNAG 1 cut(s) 510
DpnI GATC 5 cut(s) 29, 92, 482, 545, 570
DpnII GATC 5 cut(s) 27, 90, 480, 543, 568
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
Eco130I CCWWGG 2 cut(s) 352, 426
Eco32I GATATC 1 cut(s) 286
Eco47I GGWCC 3 cut(s) 59, 239, 521
Eco57I CTGAAG 2 cut(s) 426, 474
EcoO109I RGGNCCY 1 cut(s) 59
EcoRI GAATTC 1 cut(s) 164
EcoRV GATATC 1 cut(s) 286
EcoT14I CCWWGG 2 cut(s) 352, 426
ErhI CCWWGG 2 cut(s) 352, 426
FaeI CATG 2 cut(s) 299, 317
FalI AAGNNNNNCTT 2 cut(s) 106, 138
FaqI GGGAC 1 cut(s) 574
FatI CATG 2 cut(s) 295, 313
FauI CCCGC 1 cut(s) 572
FbaI TGATCA 2 cut(s) 27, 543
Fnu4HI GCNGC 1 cut(s) 554
Fsp4HI GCNGC 1 cut(s) 554
FspBI CTAG 2 cut(s) 54, 573
FspI TGCGCA 1 cut(s) 102
GlaI GCGC 2 cut(s) 102, 473
GluI GCNGC 1 cut(s) 554
HapII CCGG 1 cut(s) 199
HgaI GACGC 1 cut(s) 452
HhaI GCGC 2 cut(s) 103, 474
Hin1II CATG 2 cut(s) 299, 317
Hin6I GCGC 2 cut(s) 101, 472
HinP1I GCGC 2 cut(s) 101, 472
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 4 cut(s) 8, 13, 292, 431
HpaII CCGG 1 cut(s) 199
HphI GGTGA 2 cut(s) 22, 100
Hpy166II GTNNAC 1 cut(s) 598
Hpy188I TCNGA 1 cut(s) 18
Hpy188III TCNNGA 3 cut(s) 289, 314, 512
Hpy8I GTNNAC 1 cut(s) 598
HpyAV CCTTC 1 cut(s) 274
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4V TGCA 2 cut(s) 152, 553
HpyF10VI GCNNNNNNNGC 1 cut(s) 265
HpyF3I CTNAG 1 cut(s) 510
Hsp92II CATG 2 cut(s) 299, 317
HspAI GCGC 2 cut(s) 101, 472
Ksp22I TGATCA 2 cut(s) 27, 543
Kzo9I GATC 5 cut(s) 27, 90, 480, 543, 568
LpnPI CCDG 7 cut(s) 75, 212, 219, 322, 386, 497, 510
Lsp1109I GCAGC 1 cut(s) 565
LweI GCATC 1 cut(s) 199
MaeI CTAG 2 cut(s) 54, 573
MalI GATC 5 cut(s) 29, 92, 482, 545, 570
MboI GATC 5 cut(s) 27, 90, 480, 543, 568
MboII GAAGA 2 cut(s) 31, 42
MflI RGATCY 1 cut(s) 480
MluCI AATT 5 cut(s) 164, 177, 219, 539, 622
MmeI TCCRAC 2 cut(s) 329, 567
MnlI CCTC 5 cut(s) 67, 110, 363, 534, 569
MseI TTAA 7 cut(s) 45, 176, 255, 332, 485, 503, 537
MspI CCGG 1 cut(s) 199
MspR9I CCNGG 1 cut(s) 200
MwoI GCNNNNNNNGC 1 cut(s) 265
NciI CCSGG 1 cut(s) 200
NdeII GATC 5 cut(s) 27, 90, 480, 543, 568
NlaIII CATG 2 cut(s) 299, 317
NlaIV GGNNCC 2 cut(s) 60, 204
NsbI TGCGCA 1 cut(s) 102
PagI TCATGA 1 cut(s) 313
PfeI GAWTC 4 cut(s) 8, 13, 292, 431
PkrI GCNGC 1 cut(s) 555
PpuMI RGGWCCY 1 cut(s) 59
Psp5II RGGWCCY 1 cut(s) 59
PspN4I GGNNCC 2 cut(s) 60, 204
PspPI GGNCC 3 cut(s) 59, 239, 521
PspPPI RGGWCCY 1 cut(s) 59
PstI CTGCAG 1 cut(s) 555
PsuI RGATCY 1 cut(s) 480
RsaI GTAC 2 cut(s) 107, 374
RsaNI GTAC 2 cut(s) 106, 373
SaqAI TTAA 7 cut(s) 45, 176, 255, 332, 485, 503, 537
SatI GCNGC 1 cut(s) 554
Sau3AI GATC 5 cut(s) 27, 90, 480, 543, 568
Sau96I GGNCC 3 cut(s) 59, 239, 521
ScaI AGTACT 1 cut(s) 107
ScrFI CCNGG 1 cut(s) 200
SetI ASST 9 cut(s) 235, 244, 261, 270, 321, 327, 374, 442, 606
SfaNI GCATC 1 cut(s) 199
SfcI CTRYAG 2 cut(s) 476, 551
SinI GGWCC 3 cut(s) 59, 239, 521
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 5 cut(s) 164, 177, 219, 539, 622
SsiI CCGC 1 cut(s) 579
SspMI CTAG 2 cut(s) 54, 573
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 2 cut(s) 352, 426
TaaI ACNGT 1 cut(s) 490
TaqI TCGA 1 cut(s) 11
TasI AATT 5 cut(s) 164, 177, 219, 539, 622
TatI WGTACW 1 cut(s) 105
TfiI GAWTC 4 cut(s) 8, 13, 292, 431
Tru1I TTAA 7 cut(s) 45, 176, 255, 332, 485, 503, 537
Tru9I TTAA 7 cut(s) 45, 176, 255, 332, 485, 503, 537
TseI GCWGC 1 cut(s) 553
TspDTI ATGAA 3 cut(s) 176, 615, 634
VpaK11BI GGWCC 3 cut(s) 59, 239, 521
XapI RAATTY 1 cut(s) 164
XspI CTAG 2 cut(s) 54, 573
ZrmI AGTACT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.