Rh5BG351900

Ribosome production factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002361
Physical Location & Seq
Reverse (-)
54254399 .. 54263054
8656 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG351900.1

Sequence Viewer

Length: 714 bp
ATGGGAGTCAAGGACAAGAAGAAGAACAAGGAGCTTGCCCAGAGAAATTTAGAGCCTGTTGAAAAGGATAAAAGTTCAGGACCTTCAGAGATGAGAAAGAGAAAAAGAGAGAAGAAAACAATGGTGATTCGAGAAAAGTTGTTCAGATCTCTATTGAAGAGGGGTCCTGCTTTTATAGAGGAACTAGTTTTGGTGATCCCAAATGCGCAGTACTATAAAAGAGGCACTTATGACTTGAAAAAGATTATAGAGTATGCAAATAAAATGGAATTCACTTCTTTAATTGTTGTTCATACCAATCGCTGGGAACCAGATGCTCTCCTAATTATTGGCTTACCTAATGGACCTACTACCCATTTTAAGCTCTCAAAGCTTGTTTTACGGAAGGATATCAAGAATCATGGAAATCCAACCAGTCATGAGCCTGAGCTTCGAGATTTTATATTCTTCCGGCATCATCGGTACATTTTTGAAACCAAAGAGACTAAACAGGCTGAAGTAAAGGGCAAAAAGACCAAGGATTCCAAAGCTGACGCTATTACTGAAGCAAAACCATTTTCGCGCCTACAGAAAAATTTCAATGGTTCCCATCACCCAATTATTGGCTTTCCGTTTGGTCCTCGGCCAGTCAACTTAGGTGGTCATGGCCCTCATGGCATGCCATCGCCAGGTCAAGCAGTTCTCTTTGCATTTGTTATTGGTACTCGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Brix

Protein Analysis

237

Amino Acids

27.13

Weight (kDa)

10.11

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Brix PF04427 57 - 144 1.2e-10 Brix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 207
AccB7I CCANNNNNTGG 2 cut(s) 303, 602
AccII CGCG 1 cut(s) 562
AclWI GGATC 1 cut(s) 190
AcoI YGGCCR 1 cut(s) 623
AcsI RAATTY 3 cut(s) 46, 269, 574
AcuI CTGAAG 3 cut(s) 69, 516, 564
AfaI GTAC 3 cut(s) 212, 464, 703
AfiI CCNNNNNNNGG 3 cut(s) 303, 602, 668
AgsI TTSAA 5 cut(s) 62, 157, 238, 473, 580
AhlI ACTAGT 1 cut(s) 184
AjnI CCWGG 1 cut(s) 667
AluBI AGCT 5 cut(s) 34, 364, 373, 430, 530
AluI AGCT 5 cut(s) 34, 364, 373, 430, 530
Alw26I GTCTC 1 cut(s) 476
AlwI GGATC 1 cut(s) 190
AoxI GGCC 2 cut(s) 623, 646
ApoI RAATTY 3 cut(s) 46, 269, 574
Asp700I GAANNNNTTC 1 cut(s) 575
AspLEI GCGC 2 cut(s) 208, 564
AspS9I GGNCC 5 cut(s) 80, 164, 344, 617, 647
AsuHPI GGTGA 3 cut(s) 136, 205, 584
AvaII GGWCC 4 cut(s) 80, 164, 344, 617
BauI CACGAG 1 cut(s) 705
BccI CCATC 2 cut(s) 597, 670
BciT130I CCWGG 1 cut(s) 669
BcoDI GTCTC 1 cut(s) 476
BcuI ACTAGT 1 cut(s) 184
BfaI CTAG 1 cut(s) 185
BfmI CTRYAG 1 cut(s) 566
BglI GCCNNNNNGGC 1 cut(s) 654
BglII AGATCT 1 cut(s) 146
BmcAI AGTACT 1 cut(s) 212
Bme1390I CCNGG 1 cut(s) 669
Bme18I GGWCC 4 cut(s) 80, 164, 344, 617
BmgT120I GGNCC 5 cut(s) 80, 164, 344, 617, 647
BmiI GGNNCC 3 cut(s) 165, 309, 586
BmrFI CCNGG 1 cut(s) 669
BmsI GCATC 2 cut(s) 304, 463
Bpu10I CCTNAGC 1 cut(s) 426
BsaJI CCNNGG 2 cut(s) 516, 620
Bsc4I CCNNNNNNNGG 3 cut(s) 303, 602, 668
Bse1I ACTGG 2 cut(s) 414, 626
BseBI CCWGG 1 cut(s) 669
BseDI CCNNGG 2 cut(s) 516, 620
BseLI CCNNNNNNNGG 3 cut(s) 303, 602, 668
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 2 cut(s) 414, 626
BseYI CCCAGC 1 cut(s) 303
Bsh1236I CGCG 1 cut(s) 562
BshFI GGCC 2 cut(s) 625, 648
BsiSI CCGG 1 cut(s) 451
BslI CCNNNNNNNGG 3 cut(s) 303, 602, 668
BsmAI GTCTC 1 cut(s) 476
BsnI GGCC 2 cut(s) 625, 648
Bsp143I GATC 2 cut(s) 146, 195
BspANI GGCC 2 cut(s) 625, 648
BspCNI CTCAG 1 cut(s) 418
BspFNI CGCG 1 cut(s) 562
BspHI TCATGA 1 cut(s) 418
BspLI GGNNCC 3 cut(s) 165, 309, 586
BspPI GGATC 1 cut(s) 190
BsrI ACTGG 2 cut(s) 414, 626
BssECI CCNNGG 2 cut(s) 516, 620
BssMI GATC 2 cut(s) 146, 195
BssSI CACGAG 1 cut(s) 705
BssT1I CCWWGG 1 cut(s) 516
Bst2BI CACGAG 1 cut(s) 705
Bst2UI CCWGG 1 cut(s) 669
Bst6I CTCTTC 1 cut(s) 152
BstC8I GCNNGC 2 cut(s) 36, 659
BstDEI CTNAG 2 cut(s) 426, 634
BstFNI CGCG 1 cut(s) 562
BstHHI GCGC 2 cut(s) 208, 564
BstKTI GATC 2 cut(s) 149, 198
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 2 cut(s) 146, 195
BstMWI GCNNNNNNNGC 2 cut(s) 370, 654
BstNI CCWGG 1 cut(s) 669
BstNSI RCATGY 1 cut(s) 661
BstSCI CCNGG 1 cut(s) 667
BstSFI CTRYAG 1 cut(s) 566
BstUI CGCG 1 cut(s) 562
BstX2I RGATCY 1 cut(s) 146
BstYI RGATCY 1 cut(s) 146
BsuRI GGCC 2 cut(s) 625, 648
BtgZI GCGATG 1 cut(s) 648
Cac8I GCNNGC 2 cut(s) 36, 659
CciI TCATGA 1 cut(s) 418
CfoI GCGC 2 cut(s) 208, 564
Cfr13I GGNCC 5 cut(s) 80, 164, 344, 617, 647
CseI GACGC 1 cut(s) 542
Csp6I GTAC 3 cut(s) 211, 463, 702
CspCI CAANNNNNGTGG 2 cut(s) 619, 654
CviAII CATG 5 cut(s) 401, 419, 644, 653, 658
CviQI GTAC 3 cut(s) 211, 463, 702
DdeI CTNAG 2 cut(s) 426, 634
DpnI GATC 2 cut(s) 148, 197
DpnII GATC 2 cut(s) 146, 195
EaeI YGGCCR 1 cut(s) 623
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 516
Eco32I GATATC 1 cut(s) 391
Eco47I GGWCC 4 cut(s) 80, 164, 344, 617
Eco57I CTGAAG 3 cut(s) 69, 516, 564
EcoO109I RGGNCCY 2 cut(s) 80, 164
EcoRI GAATTC 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 667
EcoRV GATATC 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 516
ErhI CCWWGG 1 cut(s) 516
FaeI CATG 5 cut(s) 404, 422, 647, 656, 661
FalI AAGNNNNNCTT 2 cut(s) 211, 243
FatI CATG 5 cut(s) 400, 418, 643, 652, 657
FspBI CTAG 1 cut(s) 185
FspI TGCGCA 1 cut(s) 207
GlaI GCGC 2 cut(s) 207, 563
GsaI CCCAGC 1 cut(s) 307
HaeIII GGCC 2 cut(s) 625, 648
HapII CCGG 1 cut(s) 451
HgaI GACGC 1 cut(s) 542
HhaI GCGC 2 cut(s) 208, 564
Hin1II CATG 5 cut(s) 404, 422, 647, 656, 661
Hin6I GCGC 2 cut(s) 206, 562
HinP1I GCGC 2 cut(s) 206, 562
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 4 cut(s) 6, 127, 397, 521
HpaII CCGG 1 cut(s) 451
HphI GGTGA 3 cut(s) 136, 205, 584
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 2 cut(s) 88, 146
Hpy188III TCNNGA 5 cut(s) 78, 131, 394, 419, 434
Hpy8I GTNNAC 1 cut(s) 631
HpyAV CCTTC 2 cut(s) 93, 379
HpyCH4V TGCA 2 cut(s) 257, 689
HpyF10VI GCNNNNNNNGC 2 cut(s) 370, 654
HpyF3I CTNAG 2 cut(s) 426, 634
Hsp92II CATG 5 cut(s) 404, 422, 647, 656, 661
HspAI GCGC 2 cut(s) 206, 562
Kzo9I GATC 2 cut(s) 146, 195
LmnI GCTCC 1 cut(s) 31
LweI GCATC 2 cut(s) 304, 463
MaeI CTAG 1 cut(s) 185
MalI GATC 2 cut(s) 148, 197
MboI GATC 2 cut(s) 146, 195
MboII GAAGA 5 cut(s) 31, 34, 124, 169, 439
MflI RGATCY 1 cut(s) 146
MluCI AATT 6 cut(s) 46, 269, 282, 324, 574, 597
MlyI GAGTC 1 cut(s) 15
MmeI TCCRAC 1 cut(s) 434
MnlI CCTC 5 cut(s) 153, 172, 215, 630, 660
MroXI GAANNNNTTC 1 cut(s) 575
MseI TTAA 2 cut(s) 281, 360
MspI CCGG 1 cut(s) 451
MspR9I CCNGG 1 cut(s) 669
MvaI CCWGG 1 cut(s) 669
MvnI CGCG 1 cut(s) 562
MwoI GCNNNNNNNGC 2 cut(s) 370, 654
NdeII GATC 2 cut(s) 146, 195
NlaIII CATG 5 cut(s) 404, 422, 647, 656, 661
NlaIV GGNNCC 3 cut(s) 165, 309, 586
NmeAIII GCCGAG 1 cut(s) 601
NsbI TGCGCA 1 cut(s) 207
NspI RCATGY 1 cut(s) 661
PaeI GCATGC 1 cut(s) 661
PagI TCATGA 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 575
PfeI GAWTC 3 cut(s) 127, 397, 521
PflMI CCANNNNNTGG 2 cut(s) 303, 602
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
PpuMI RGGWCCY 2 cut(s) 80, 164
Psp5II RGGWCCY 2 cut(s) 80, 164
Psp6I CCWGG 1 cut(s) 667
PspFI CCCAGC 1 cut(s) 303
PspGI CCWGG 1 cut(s) 667
PspN4I GGNNCC 3 cut(s) 165, 309, 586
PspPI GGNCC 5 cut(s) 80, 164, 344, 617, 647
PspPPI RGGWCCY 2 cut(s) 80, 164
PsuI RGATCY 1 cut(s) 146
RsaI GTAC 3 cut(s) 212, 464, 703
RsaNI GTAC 3 cut(s) 211, 463, 702
SaqAI TTAA 2 cut(s) 281, 360
Sau3AI GATC 2 cut(s) 146, 195
Sau96I GGNCC 5 cut(s) 80, 164, 344, 617, 647
ScaI AGTACT 1 cut(s) 212
SchI GAGTC 1 cut(s) 15
ScrFI CCNGG 1 cut(s) 669
SfaNI GCATC 2 cut(s) 304, 463
SfcI CTRYAG 1 cut(s) 566
SinI GGWCC 4 cut(s) 80, 164, 344, 617
SpeI ACTAGT 1 cut(s) 184
SphI GCATGC 1 cut(s) 661
Sse9I AATT 6 cut(s) 46, 269, 282, 324, 574, 597
SspMI CTAG 1 cut(s) 185
StyD4I CCNGG 1 cut(s) 667
StyI CCWWGG 1 cut(s) 516
TaqI TCGA 2 cut(s) 130, 433
TasI AATT 6 cut(s) 46, 269, 282, 324, 574, 597
TatI WGTACW 1 cut(s) 210
TfiI GAWTC 3 cut(s) 127, 397, 521
Tru1I TTAA 2 cut(s) 281, 360
Tru9I TTAA 2 cut(s) 281, 360
TspDTI ATGAA 1 cut(s) 281
TspGWI ACGGA 2 cut(s) 397, 600
Van91I CCANNNNNTGG 2 cut(s) 303, 602
VpaK11BI GGWCC 4 cut(s) 80, 164, 344, 617
XapI RAATTY 3 cut(s) 46, 269, 574
XceI RCATGY 1 cut(s) 661
XmnI GAANNNNTTC 1 cut(s) 575
XspI CTAG 1 cut(s) 185
ZrmI AGTACT 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.