Rw1G026720

Ribosome production factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002361
Physical Location & Seq
Reverse (-)
53996710 .. 54000713
4004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G026720.1

Sequence Viewer

Length: 861 bp
ATGGGCTTCAAGAGGAAGAAGAGCGAGAACGAAGGAGGAGACGAGCCCAAACCAGTAGCAGTGGAGATCGAGAAGAAGAAGAAGGGGATACTCCCGTCGATGATAAAGAACAAGGACAAGAGAGCCGCCGTCCACGCCAAGCTCAAGCAAGAGAAGAAGCTCGATAAGCGCAAGAAGGTCAAGGCCCGCGACGCCGCCGAAAAGCGAGCTCTCGAGCTCGGCGAAGAGCCTCCGGCGAGGAAGATTCCGAAGACGATTGAGAACACCAGGGAGGAGGATGAGACTGTTTGTAGGCCCGACGACGAAGAGTTGTTTGTTAGAAATGATGCGGATGAGTTTAGTGCGGTTTTGAAGCGTGAATCGAATCCGAAGATATTGATCACCACTTGCCGATTTAACTCTTCTAGGGGTCCTGCTTTTATAGAGGAACTACTTTCGGTGATCCCAAATGCGCAGTACTATAAAAGAGGCACTTATGACTTGAAAAAGATTATAGAGTATGCAAATAAAAAGGAATTCACTTCTTTAATTGTTGTTCATACCAATCGCCGGGAACCAGATGCTCTCCTAATAATTGGCTTACCTAATGGACCGACTGCCCATTTTAAGCTCTCAAAGCTCGTTTTACGGAAGGATATCAAGAATCATGGAAATCCAACCAGTCATGAGCCTGAGCTTGTATTAAACAACTTTACAACACGCCTTGGTCATCGTATTGGGAGATTAATACAGTCACTTTTCCCTCAAGAACCAAATTTTCGTGGTCGGCGAGTTGTAACTTTCCACAACCAGCGAGATTTTATATTCTTCCGGCATCATCGGTACATTTTGAAACCAAAGAAACTAAACAGGCTGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Brix

Protein Analysis

286

Amino Acids

33.21

Weight (kDa)

10.0

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Brix PF04427 133 - 278 2.7e-26 Brix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 453
AccII CGCG 1 cut(s) 189
AciI CCGC 5 cut(s) 126, 187, 195, 329, 344
AclWI GGATC 1 cut(s) 436
AcsI RAATTY 2 cut(s) 515, 754
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 2 cut(s) 458, 824
AfiI CCNNNNNNNGG 1 cut(s) 549
AgsI TTSAA 4 cut(s) 10, 352, 484, 832
AjnI CCWGG 1 cut(s) 266
AluBI AGCT 7 cut(s) 142, 160, 209, 217, 610, 619, 676
AluI AGCT 7 cut(s) 142, 160, 209, 217, 610, 619, 676
Alw21I GWGCWC 2 cut(s) 211, 219
Alw26I GTCTC 2 cut(s) 33, 275
AlwI GGATC 1 cut(s) 436
Ama87I CYCGRG 1 cut(s) 212
AoxI GGCC 2 cut(s) 183, 293
ApoI RAATTY 2 cut(s) 515, 754
AseI ATTAAT 1 cut(s) 725
AspLEI GCGC 2 cut(s) 171, 454
AspS9I GGNCC 4 cut(s) 184, 294, 410, 590
AsuC2I CCSGG 1 cut(s) 551
AsuHPI GGTGA 2 cut(s) 373, 451
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 2 cut(s) 410, 590
BaeI ACNNNNGTAYC 2 cut(s) 80, 113
BanII GRGCYC 3 cut(s) 48, 211, 219
BbsI GAAGAC 1 cut(s) 257
Bbv12I GWGCWC 2 cut(s) 211, 219
BceAI ACGGC 1 cut(s) 113
BciT130I CCWGG 1 cut(s) 268
BciVI GTATCC 1 cut(s) 81
BclI TGATCA 1 cut(s) 378
BcnI CCSGG 1 cut(s) 551
BcoDI GTCTC 2 cut(s) 33, 275
BfaI CTAG 1 cut(s) 405
BfuI GTATCC 1 cut(s) 81
BisI GCNGC 2 cut(s) 126, 195
BlsI GCNGC 2 cut(s) 127, 196
BmcAI AGTACT 1 cut(s) 458
Bme1390I CCNGG 2 cut(s) 268, 551
Bme18I GGWCC 2 cut(s) 410, 590
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 4 cut(s) 184, 294, 410, 590
BmiI GGNNCC 2 cut(s) 411, 555
BmrFI CCNGG 2 cut(s) 268, 551
BmsI GCATC 3 cut(s) 316, 550, 823
BpiI GAAGAC 1 cut(s) 257
Bpu10I CCTNAGC 1 cut(s) 672
BpuEI CTTGAG 2 cut(s) 128, 729
BpuMI CCSGG 1 cut(s) 551
BsaBI GATNNNNATC 1 cut(s) 377
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 2 cut(s) 267, 703
Bsc4I CCNNNNNNNGG 1 cut(s) 549
Bse1I ACTGG 2 cut(s) 53, 660
Bse8I GATNNNNATC 1 cut(s) 377
BseBI CCWGG 1 cut(s) 268
BseDI CCNNGG 2 cut(s) 267, 703
BseGI GGATG 2 cut(s) 283, 337
BseJI GATNNNNATC 1 cut(s) 377
BseLI CCNNNNNNNGG 1 cut(s) 549
BseMII CTCAG 1 cut(s) 663
BseNI ACTGG 2 cut(s) 53, 660
BseRI GAGGAG 2 cut(s) 51, 287
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 2 cut(s) 185, 295
BsiHKAI GWGCWC 2 cut(s) 211, 219
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 3 cut(s) 233, 550, 811
BslI CCNNNNNNNGG 1 cut(s) 549
BsmAI GTCTC 2 cut(s) 33, 275
BsmBI CGTCTC 1 cut(s) 33
BsnI GGCC 2 cut(s) 185, 295
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 3 cut(s) 48, 211, 219
Bsp143I GATC 3 cut(s) 66, 378, 441
BspACI CCGC 5 cut(s) 126, 187, 195, 329, 344
BspANI GGCC 2 cut(s) 185, 295
BspCNI CTCAG 1 cut(s) 664
BspFNI CGCG 1 cut(s) 189
BspHI TCATGA 1 cut(s) 664
BspLI GGNNCC 2 cut(s) 411, 555
BspPI GGATC 1 cut(s) 436
BspQI GCTCTTC 2 cut(s) 14, 219
BsrI ACTGG 2 cut(s) 53, 660
BssECI CCNNGG 2 cut(s) 267, 703
BssMI GATC 3 cut(s) 66, 378, 441
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 703
Bst2UI CCWGG 1 cut(s) 268
Bst4CI ACNGT 2 cut(s) 286, 732
Bst6I CTCTTC 4 cut(s) 14, 219, 300, 406
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 2 cut(s) 187, 207
BstDEI CTNAG 1 cut(s) 672
BstF5I GGATG 2 cut(s) 283, 337
BstFNI CGCG 1 cut(s) 189
BstHHI GCGC 2 cut(s) 171, 454
BstKTI GATC 3 cut(s) 69, 381, 444
BstMAI GTCTC 2 cut(s) 33, 275
BstMBI GATC 3 cut(s) 66, 378, 441
BstMWI GCNNNNNNNGC 4 cut(s) 134, 166, 191, 616
BstNI CCWGG 1 cut(s) 268
BstSCI CCNGG 2 cut(s) 266, 549
BstUI CGCG 1 cut(s) 189
BstV2I GAAGAC 1 cut(s) 257
BsuI GTATCC 1 cut(s) 81
BsuRI GGCC 2 cut(s) 185, 295
BtsCI GGATG 2 cut(s) 283, 337
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 1 cut(s) 66
Cac8I GCNNGC 2 cut(s) 187, 207
CciI TCATGA 1 cut(s) 664
CfoI GCGC 2 cut(s) 171, 454
Cfr13I GGNCC 4 cut(s) 184, 294, 410, 590
CseI GACGC 1 cut(s) 200
Csp6I GTAC 2 cut(s) 457, 823
CviAII CATG 2 cut(s) 647, 665
CviQI GTAC 2 cut(s) 457, 823
DdeI CTNAG 1 cut(s) 672
DpnI GATC 3 cut(s) 68, 380, 443
DpnII GATC 3 cut(s) 66, 378, 441
Eam1104I CTCTTC 4 cut(s) 14, 219, 300, 406
EarI CTCTTC 4 cut(s) 14, 219, 300, 406
Ecl136II GAGCTC 2 cut(s) 209, 217
Eco130I CCWWGG 1 cut(s) 703
Eco24I GRGCYC 3 cut(s) 48, 211, 219
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 2 cut(s) 410, 590
Eco53kI GAGCTC 2 cut(s) 209, 217
Eco88I CYCGRG 1 cut(s) 212
EcoICRI GAGCTC 2 cut(s) 209, 217
EcoO109I RGGNCCY 1 cut(s) 410
EcoRI GAATTC 1 cut(s) 515
EcoRII CCWGG 1 cut(s) 266
EcoRV GATATC 1 cut(s) 637
EcoT14I CCWWGG 1 cut(s) 703
EcoT38I GRGCYC 3 cut(s) 48, 211, 219
ErhI CCWWGG 1 cut(s) 703
Esp3I CGTCTC 1 cut(s) 33
FaeI CATG 2 cut(s) 650, 668
FaiI YATR 9 cut(s) 422, 462, 477, 494, 501, 540, 648, 666, 803
FalI AAGNNNNNCTT 2 cut(s) 457, 489
FatI CATG 2 cut(s) 646, 664
FauI CCCGC 1 cut(s) 194
FbaI TGATCA 1 cut(s) 378
Fnu4HI GCNGC 2 cut(s) 126, 195
FokI GGATG 2 cut(s) 290, 344
FriOI GRGCYC 3 cut(s) 48, 211, 219
Fsp4HI GCNGC 2 cut(s) 126, 195
FspBI CTAG 1 cut(s) 405
FspI TGCGCA 1 cut(s) 453
GlaI GCGC 2 cut(s) 170, 453
GluI GCNGC 2 cut(s) 126, 195
HaeIII GGCC 2 cut(s) 185, 295
HapII CCGG 3 cut(s) 233, 550, 811
HgaI GACGC 1 cut(s) 200
HhaI GCGC 2 cut(s) 171, 454
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 2 cut(s) 650, 668
Hin6I GCGC 2 cut(s) 169, 452
HinP1I GCGC 2 cut(s) 169, 452
HinfI GANTC 4 cut(s) 244, 359, 364, 643
HpaII CCGG 3 cut(s) 233, 550, 811
HphI GGTGA 2 cut(s) 373, 451
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 2 cut(s) 249, 369
Hpy188III TCNNGA 6 cut(s) 10, 70, 212, 640, 665, 746
Hpy8I GTNNAC 1 cut(s) 133
Hpy99I CGWCG 4 cut(s) 100, 194, 302, 305
HpyAV CCTTC 4 cut(s) 26, 76, 169, 625
HpyCH4III ACNGT 2 cut(s) 286, 732
HpyCH4V TGCA 1 cut(s) 503
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 166, 191, 616
HpyF3I CTNAG 1 cut(s) 672
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 2 cut(s) 650, 668
HspAI GCGC 2 cut(s) 169, 452
Ksp22I TGATCA 1 cut(s) 378
Kzo9I GATC 3 cut(s) 66, 378, 441
LguI GCTCTTC 2 cut(s) 14, 219
LweI GCATC 3 cut(s) 316, 550, 823
MaeI CTAG 1 cut(s) 405
MaeIII GTNAC 2 cut(s) 732, 775
MalI GATC 3 cut(s) 68, 380, 443
MboI GATC 3 cut(s) 66, 378, 441
MhlI GDGCHC 3 cut(s) 48, 211, 219
MluCI AATT 4 cut(s) 515, 528, 573, 754
MmeI TCCRAC 1 cut(s) 680
MnlI CCTC 9 cut(s) 6, 29, 231, 240, 265, 268, 418, 461, 753
MseI TTAA 5 cut(s) 396, 527, 606, 683, 725
MspI CCGG 3 cut(s) 233, 550, 811
MspR9I CCNGG 2 cut(s) 268, 551
MvaI CCWGG 1 cut(s) 268
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 4 cut(s) 134, 166, 191, 616
NciI CCSGG 1 cut(s) 551
NdeII GATC 3 cut(s) 66, 378, 441
NlaIII CATG 2 cut(s) 650, 668
NlaIV GGNNCC 2 cut(s) 411, 555
NmeAIII GCCGAG 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 732
NsbI TGCGCA 1 cut(s) 453
PaeR7I CTCGAG 1 cut(s) 212
PagI TCATGA 1 cut(s) 664
PciSI GCTCTTC 2 cut(s) 14, 219
PcsI WCGNNNNNNNCGW 2 cut(s) 219, 766
PfeI GAWTC 4 cut(s) 244, 359, 364, 643
PkrI GCNGC 2 cut(s) 127, 196
PpuMI RGGWCCY 1 cut(s) 410
PshBI ATTAAT 1 cut(s) 725
Psp124BI GAGCTC 2 cut(s) 211, 219
Psp5II RGGWCCY 1 cut(s) 410
Psp6I CCWGG 1 cut(s) 266
PspGI CCWGG 1 cut(s) 266
PspN4I GGNNCC 2 cut(s) 411, 555
PspPI GGNCC 4 cut(s) 184, 294, 410, 590
PspPPI RGGWCCY 1 cut(s) 410
RsaI GTAC 2 cut(s) 458, 824
RsaNI GTAC 2 cut(s) 457, 823
SacI GAGCTC 2 cut(s) 211, 219
SapI GCTCTTC 2 cut(s) 14, 219
SaqAI TTAA 5 cut(s) 396, 527, 606, 683, 725
SatI GCNGC 2 cut(s) 126, 195
Sau3AI GATC 3 cut(s) 66, 378, 441
Sau96I GGNCC 4 cut(s) 184, 294, 410, 590
ScaI AGTACT 1 cut(s) 458
ScrFI CCNGG 2 cut(s) 268, 551
SduI GDGCHC 3 cut(s) 48, 211, 219
SetI ASST 9 cut(s) 144, 162, 180, 211, 219, 586, 612, 621, 678
SfaNI GCATC 3 cut(s) 316, 550, 823
Sfr274I CTCGAG 1 cut(s) 212
SinI GGWCC 2 cut(s) 410, 590
SlaI CTCGAG 1 cut(s) 212
SmlI CTYRAG 3 cut(s) 143, 212, 744
SmoI CTYRAG 3 cut(s) 143, 212, 744
Sse9I AATT 4 cut(s) 515, 528, 573, 754
SsiI CCGC 5 cut(s) 126, 187, 195, 329, 344
SspMI CTAG 1 cut(s) 405
SstI GAGCTC 2 cut(s) 211, 219
StyD4I CCNGG 2 cut(s) 266, 549
StyI CCWWGG 1 cut(s) 703
TaaI ACNGT 2 cut(s) 286, 732
TaqI TCGA 5 cut(s) 69, 98, 162, 213, 362
TaqII GACCGA 1 cut(s) 607
TasI AATT 4 cut(s) 515, 528, 573, 754
TatI WGTACW 1 cut(s) 456
TauI GCSGC 2 cut(s) 128, 197
TfiI GAWTC 4 cut(s) 244, 359, 364, 643
Tru1I TTAA 5 cut(s) 396, 527, 606, 683, 725
Tru9I TTAA 5 cut(s) 396, 527, 606, 683, 725
TscAI CASTG 1 cut(s) 66
TseFI GTSAC 1 cut(s) 732
Tsp45I GTSAC 1 cut(s) 732
TspDTI ATGAA 1 cut(s) 527
TspGWI ACGGA 1 cut(s) 643
TspRI CASTG 1 cut(s) 66
VpaK11BI GGWCC 2 cut(s) 410, 590
VspI ATTAAT 1 cut(s) 725
XapI RAATTY 2 cut(s) 515, 754
XhoI CTCGAG 1 cut(s) 212
XspI CTAG 1 cut(s) 405
ZrmI AGTACT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.