Rw5G034050

Ribosome production factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002361
Physical Location & Seq
Forward (+)
58314512 .. 58318747
4236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G034050.1

Sequence Viewer

Length: 930 bp
ATGAGAAAGAGAAAAAGAGAGAAGAAAACAATGGTGATTCGAGAAAAGTTGTTCAGATCTCTATTGAAGGTAAAGTGGAGTTCTAGCTTTAAACTGAGGAAAGGAAAAGGAAAGAAGGTGAAGAACAATCAGGATTTGGATGATCCGTCTAATACCGATGCGAGTTTGCAGTTGTTTGTTAGAAATGATGCAGATGAGTTTAGTGCGGTTTTGAAGCGTGAATCGAATCCGAAGATATTGATCACCACTTGCCGATTTAACTCTTCTAGGGGTCCTGCTTTTATAGAGGAACTACTTTCGGTGATCCCAAATGCGCAGTACTATAAAAGCGGCACTTATGACTTGAAAAAGATTATAGAGTATGCAAATAAAAAGGAATTCACTTCTTTAATTGTTGTTCATACCAATCGCCGGGAACCAGATGTTCTCCTAATTATTGGCTTACCTAATGGACCTATTGCCCATTTTAAGCTCTCAAAGCTTGTTTTACGGAAGGATATCAATAATCATGGAAATCCAACCAGTCATGAGCCTGAGCTTGTATTAAACAACTTTACAACACGCCTTGGTCATCGTATTGGGAGATTAATACAGTCACTTTTCCCTCAAGAACCAAATTTTCGTGGTCGGCGAGTTGTAACTTTCCACAACCAGCGAGATTTTATGTTCTTCCGGCATCATCGGTACATTTTTGAAACCAAAGAGACTAAACAGGTTGAAGTAAAGGGCAAAAAGACCAAGGATTCCAAAGCTGACGCTATTACTGAAGCAAAACCATTTGCTCGCCTACAGGAATGTGGTCCTCGTTTTACCCTTAAATTGATCAGTCTGCAGCACGGGACATTTGATCCTAGAGGCGGGGAGTTTGAGTGGGTTCACAAGACGACGGTGAGTCTTCAAATCAGACGACGTTTTTTCACCGTCGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Brix

Protein Analysis

309

Amino Acids

36.06

Weight (kDa)

10.31

Isoelectric Point (pI)

37.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Brix PF04427 87 - 275 7.1e-31 Brix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 315
AciI CCGC 3 cut(s) 206, 330, 858
AclWI GGATC 3 cut(s) 137, 298, 842
AcsI RAATTY 2 cut(s) 377, 616
AcuI CTGAAG 1 cut(s) 786
AfaI GTAC 2 cut(s) 320, 686
AfiI CCNNNNNNNGG 2 cut(s) 411, 857
AgsI TTSAA 6 cut(s) 67, 214, 346, 695, 719, 899
AhdI GACNNNNNGTC 1 cut(s) 891
AleI CACNNNNGTG 1 cut(s) 923
AluBI AGCT 5 cut(s) 87, 472, 481, 538, 752
AluI AGCT 5 cut(s) 87, 472, 481, 538, 752
Alw26I GTCTC 1 cut(s) 698
AlwI GGATC 3 cut(s) 137, 298, 842
ApeKI GCWGC 1 cut(s) 832
ApoI RAATTY 2 cut(s) 377, 616
AseI ATTAAT 1 cut(s) 587
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 3 cut(s) 272, 452, 800
AsuC2I CCSGG 1 cut(s) 413
AsuHPI GGTGA 6 cut(s) 46, 130, 235, 313, 901, 910
AvaII GGWCC 3 cut(s) 272, 452, 800
BbsI GAAGAC 1 cut(s) 887
BbvI GCAGC 1 cut(s) 844
BclI TGATCA 2 cut(s) 240, 822
BcnI CCSGG 1 cut(s) 413
BcoDI GTCTC 1 cut(s) 698
BfaI CTAG 3 cut(s) 84, 267, 852
BfmI CTRYAG 2 cut(s) 788, 830
BglII AGATCT 1 cut(s) 56
BisI GCNGC 2 cut(s) 331, 833
BlsI GCNGC 2 cut(s) 332, 834
BmcAI AGTACT 1 cut(s) 320
Bme1390I CCNGG 1 cut(s) 413
Bme18I GGWCC 3 cut(s) 272, 452, 800
BmeRI GACNNNNNGTC 1 cut(s) 891
BmgT120I GGNCC 3 cut(s) 272, 452, 800
BmiI GGNNCC 2 cut(s) 273, 417
BmrFI CCNGG 1 cut(s) 413
BmsI GCATC 3 cut(s) 148, 178, 685
BpiI GAAGAC 1 cut(s) 887
Bpu10I CCTNAGC 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 591
BpuMI CCSGG 1 cut(s) 413
BsaBI GATNNNNATC 1 cut(s) 239
BsaJI CCNNGG 2 cut(s) 565, 738
BsaXI ACNNNNNCTCC 2 cut(s) 854, 884
Bsc4I CCNNNNNNNGG 2 cut(s) 411, 857
Bse1I ACTGG 1 cut(s) 522
Bse8I GATNNNNATC 1 cut(s) 239
BseDI CCNNGG 2 cut(s) 565, 738
BseGI GGATG 1 cut(s) 145
BseJI GATNNNNATC 1 cut(s) 239
BseLI CCNNNNNNNGG 2 cut(s) 411, 857
BseMII CTCAG 2 cut(s) 86, 525
BseNI ACTGG 1 cut(s) 522
BseXI GCAGC 1 cut(s) 844
BsiSI CCGG 2 cut(s) 412, 673
BslFI GGGAC 1 cut(s) 853
BslI CCNNNNNNNGG 2 cut(s) 411, 857
BsmAI GTCTC 1 cut(s) 698
BsmFI GGGAC 1 cut(s) 853
Bsp143I GATC 6 cut(s) 56, 142, 240, 303, 822, 847
BspACI CCGC 3 cut(s) 206, 330, 858
BspCNI CTCAG 2 cut(s) 87, 526
BspHI TCATGA 1 cut(s) 526
BspLI GGNNCC 2 cut(s) 273, 417
BspMAI CTGCAG 1 cut(s) 834
BspPI GGATC 3 cut(s) 137, 298, 842
BsrI ACTGG 1 cut(s) 522
BssECI CCNNGG 2 cut(s) 565, 738
BssMI GATC 6 cut(s) 56, 142, 240, 303, 822, 847
BssT1I CCWWGG 2 cut(s) 565, 738
Bst4CI ACNGT 3 cut(s) 594, 889, 922
Bst6I CTCTTC 1 cut(s) 268
BstC8I GCNNGC 1 cut(s) 784
BstDEI CTNAG 2 cut(s) 95, 534
BstF5I GGATG 1 cut(s) 145
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 6 cut(s) 59, 145, 243, 306, 825, 850
BstMAI GTCTC 1 cut(s) 698
BstMBI GATC 6 cut(s) 56, 142, 240, 303, 822, 847
BstMWI GCNNNNNNNGC 1 cut(s) 478
BstSCI CCNGG 1 cut(s) 411
BstSFI CTRYAG 2 cut(s) 788, 830
BstV1I GCAGC 1 cut(s) 844
BstV2I GAAGAC 1 cut(s) 887
BstX2I RGATCY 1 cut(s) 56
BstYI RGATCY 1 cut(s) 56
BtsCI GGATG 1 cut(s) 145
Cac8I GCNNGC 1 cut(s) 784
CciI TCATGA 1 cut(s) 526
CfoI GCGC 1 cut(s) 316
Cfr13I GGNCC 3 cut(s) 272, 452, 800
CseI GACGC 1 cut(s) 764
Csp6I GTAC 2 cut(s) 319, 685
CviAII CATG 2 cut(s) 509, 527
CviJI RGCY 7 cut(s) 87, 441, 472, 481, 532, 538, 752
CviKI_1 RGCY 7 cut(s) 87, 441, 472, 481, 532, 538, 752
CviQI GTAC 2 cut(s) 319, 685
DdeI CTNAG 2 cut(s) 95, 534
DpnI GATC 6 cut(s) 58, 144, 242, 305, 824, 849
DpnII GATC 6 cut(s) 56, 142, 240, 303, 822, 847
DraI TTTAAA 1 cut(s) 91
DriI GACNNNNNGTC 1 cut(s) 891
Eam1104I CTCTTC 1 cut(s) 268
Eam1105I GACNNNNNGTC 1 cut(s) 891
EarI CTCTTC 1 cut(s) 268
Eco130I CCWWGG 2 cut(s) 565, 738
Eco32I GATATC 1 cut(s) 499
Eco47I GGWCC 3 cut(s) 272, 452, 800
Eco57I CTGAAG 1 cut(s) 786
EcoO109I RGGNCCY 1 cut(s) 272
EcoRI GAATTC 1 cut(s) 377
EcoRV GATATC 1 cut(s) 499
EcoT14I CCWWGG 2 cut(s) 565, 738
ErhI CCWWGG 2 cut(s) 565, 738
FaeI CATG 2 cut(s) 512, 530
FaiI YATR 9 cut(s) 284, 324, 339, 356, 363, 402, 510, 528, 665
FalI AAGNNNNNCTT 2 cut(s) 319, 351
FaqI GGGAC 1 cut(s) 853
FatI CATG 2 cut(s) 508, 526
FauI CCCGC 1 cut(s) 851
FbaI TGATCA 2 cut(s) 240, 822
Fnu4HI GCNGC 2 cut(s) 331, 833
FokI GGATG 1 cut(s) 152
Fsp4HI GCNGC 2 cut(s) 331, 833
FspBI CTAG 3 cut(s) 84, 267, 852
FspI TGCGCA 1 cut(s) 315
GlaI GCGC 1 cut(s) 315
GluI GCNGC 2 cut(s) 331, 833
HapII CCGG 2 cut(s) 412, 673
HgaI GACGC 1 cut(s) 764
HhaI GCGC 1 cut(s) 316
Hin1II CATG 2 cut(s) 512, 530
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HindIII AAGCTT 1 cut(s) 479
HinfI GANTC 5 cut(s) 37, 221, 226, 743, 892
HpaII CCGG 2 cut(s) 412, 673
HphI GGTGA 6 cut(s) 46, 130, 235, 313, 901, 910
Hpy166II GTNNAC 1 cut(s) 877
Hpy188I TCNGA 3 cut(s) 56, 231, 905
Hpy188III TCNNGA 4 cut(s) 41, 131, 527, 608
Hpy8I GTNNAC 1 cut(s) 877
Hpy99I CGWCG 3 cut(s) 889, 912, 926
HpyAV CCTTC 3 cut(s) 61, 109, 487
HpyCH4III ACNGT 3 cut(s) 594, 889, 922
HpyCH4IV ACGT 1 cut(s) 910
HpyCH4V TGCA 4 cut(s) 169, 191, 365, 832
HpyF10VI GCNNNNNNNGC 1 cut(s) 478
HpyF3I CTNAG 2 cut(s) 95, 534
HpySE526I ACGT 1 cut(s) 910
Hsp92II CATG 2 cut(s) 512, 530
HspAI GCGC 1 cut(s) 314
Ksp22I TGATCA 2 cut(s) 240, 822
Kzo9I GATC 6 cut(s) 56, 142, 240, 303, 822, 847
Lsp1109I GCAGC 1 cut(s) 844
LweI GCATC 3 cut(s) 148, 178, 685
MaeI CTAG 3 cut(s) 84, 267, 852
MaeII ACGT 1 cut(s) 910
MaeIII GTNAC 2 cut(s) 594, 637
MalI GATC 6 cut(s) 58, 144, 242, 305, 824, 849
MboI GATC 6 cut(s) 56, 142, 240, 303, 822, 847
MboII GAAGA 6 cut(s) 34, 133, 244, 255, 661, 887
MflI RGATCY 1 cut(s) 56
MluCI AATT 5 cut(s) 377, 390, 432, 616, 818
MlyI GAGTC 1 cut(s) 901
MmeI TCCRAC 1 cut(s) 542
MnlI CCTC 5 cut(s) 90, 280, 615, 813, 848
MseI TTAA 7 cut(s) 90, 258, 389, 468, 545, 587, 816
MslI CAYNNNNRTG 1 cut(s) 923
MspI CCGG 2 cut(s) 412, 673
MspR9I CCNGG 1 cut(s) 413
MwoI GCNNNNNNNGC 1 cut(s) 478
NciI CCSGG 1 cut(s) 413
NdeII GATC 6 cut(s) 56, 142, 240, 303, 822, 847
NlaIII CATG 2 cut(s) 512, 530
NlaIV GGNNCC 2 cut(s) 273, 417
NmuCI GTSAC 1 cut(s) 594
NsbI TGCGCA 1 cut(s) 315
OliI CACNNNNGTG 1 cut(s) 923
PagI TCATGA 1 cut(s) 526
PcsI WCGNNNNNNNCGW 1 cut(s) 628
PfeI GAWTC 4 cut(s) 37, 221, 226, 743
PkrI GCNGC 2 cut(s) 332, 834
PleI GAGTC 1 cut(s) 900
PpsI GAGTC 1 cut(s) 900
PpuMI RGGWCCY 1 cut(s) 272
PshBI ATTAAT 1 cut(s) 587
Psp5II RGGWCCY 1 cut(s) 272
PspN4I GGNNCC 2 cut(s) 273, 417
PspPI GGNCC 3 cut(s) 272, 452, 800
PspPPI RGGWCCY 1 cut(s) 272
PstI CTGCAG 1 cut(s) 834
PsuI RGATCY 1 cut(s) 56
RsaI GTAC 2 cut(s) 320, 686
RsaNI GTAC 2 cut(s) 319, 685
RseI CAYNNNNRTG 1 cut(s) 923
SaqAI TTAA 7 cut(s) 90, 258, 389, 468, 545, 587, 816
SatI GCNGC 2 cut(s) 331, 833
Sau3AI GATC 6 cut(s) 56, 142, 240, 303, 822, 847
Sau96I GGNCC 3 cut(s) 272, 452, 800
ScaI AGTACT 1 cut(s) 320
SchI GAGTC 1 cut(s) 901
ScrFI CCNGG 1 cut(s) 413
SfaNI GCATC 3 cut(s) 148, 178, 685
SfcI CTRYAG 2 cut(s) 788, 830
SinI GGWCC 3 cut(s) 272, 452, 800
SmiMI CAYNNNNRTG 1 cut(s) 923
SmlI CTYRAG 1 cut(s) 606
SmoI CTYRAG 1 cut(s) 606
Sse9I AATT 5 cut(s) 377, 390, 432, 616, 818
SsiI CCGC 3 cut(s) 206, 330, 858
SspMI CTAG 3 cut(s) 84, 267, 852
StyD4I CCNGG 1 cut(s) 411
StyI CCWWGG 2 cut(s) 565, 738
TaaI ACNGT 3 cut(s) 594, 889, 922
TaiI ACGT 1 cut(s) 913
TaqI TCGA 2 cut(s) 40, 224
TasI AATT 5 cut(s) 377, 390, 432, 616, 818
TatI WGTACW 1 cut(s) 318
TauI GCSGC 1 cut(s) 333
TfiI GAWTC 4 cut(s) 37, 221, 226, 743
Tru1I TTAA 7 cut(s) 90, 258, 389, 468, 545, 587, 816
Tru9I TTAA 7 cut(s) 90, 258, 389, 468, 545, 587, 816
TseFI GTSAC 1 cut(s) 594
TseI GCWGC 1 cut(s) 832
Tsp45I GTSAC 1 cut(s) 594
TspDTI ATGAA 1 cut(s) 389
TspGWI ACGGA 2 cut(s) 135, 505
VpaK11BI GGWCC 3 cut(s) 272, 452, 800
VspI ATTAAT 1 cut(s) 587
XapI RAATTY 2 cut(s) 377, 616
XspI CTAG 3 cut(s) 84, 267, 852
ZrmI AGTACT 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.