RchiOBHm_Chr4g0406331

Ribosome production factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002361
Physical Location & Seq
Reverse (-)
27528143 .. 27530055
1913 bp
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UTR
Exon/CDS
Intron
PRQ37770

Sequence Viewer

Length: 690 bp
ATGGTGGCAGTTTTGAAGCGTGAATCGAATCCGAAGATATTGATCACCACTTGCCGATTTAACTCTTCTGGCTTTTATAGAGGAACTACTTTCGGTGATCCAAATGCGCAGTACTATAAAATAGGCACTTATGACTTGAAAAAGGTACCATTTTTGTCTTTTATATCAATTTGTTTTTCACTTTTTCAAATAATTATAGAGTATGCAAATAAAAAGGAATTCACTTCTTTAATTGTTGTTCATACCAATCGCCGGGAACCAGATGCACTCCTAATTATTGGCTTACCTAATGGACCTACTGCCTATTTTAAGCTCTCAAAGCTTGTTTTACGGAAGGATATCAAGTCACTTTTCCCTCAAGAACCAAATTTTCATGGTCGGCGAGTTGTAACTTTCCACAACCAGCGAGATTTTATATTCTTCCGGCATCATCGGTACATTTTTGAAACCAAAGAGACTAAACAGGCTGAAGTAAAGGGCAAAAAGACCAAAGATTCCAAAGCTGACGCTATTACTGAAGCAAAACCATTTGCGCGCCTACAGGAATGTGGTCCTCGTTTTACCCTTAAATTGATCAGTCTGCAGGACAGGACATTTGATCCTAGAGGCGGGGAGTTTGAGTGGGTTCACAAGGTAATCCACTTATCAGTCAATTCATTTCAGTATATTGGTTTCATATCTTTATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Brix

Protein Analysis

229

Amino Acids

26.6

Weight (kDa)

9.81

Isoelectric Point (pI)

33.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Brix PF04427 115 - 192 2.3e-11 Brix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 108
Acc65I GGTACC 1 cut(s) 145
AccB1I GGYRCC 1 cut(s) 145
AccII CGCG 1 cut(s) 535
AciI CCGC 1 cut(s) 609
AclWI GGATC 2 cut(s) 92, 593
AcsI RAATTY 2 cut(s) 218, 367
AcuI CTGAAG 2 cut(s) 489, 537
AfaI GTAC 3 cut(s) 113, 147, 437
AfiI CCNNNNNNNGG 2 cut(s) 252, 608
AgsI TTSAA 4 cut(s) 16, 139, 188, 446
AluBI AGCT 3 cut(s) 313, 322, 503
AluI AGCT 3 cut(s) 313, 322, 503
Alw26I GTCTC 1 cut(s) 449
AlwI GGATC 2 cut(s) 92, 593
ApoI RAATTY 2 cut(s) 218, 367
ArsI GACNNNNNNTTYG 2 cut(s) 578, 610
Asp718I GGTACC 1 cut(s) 145
AspLEI GCGC 3 cut(s) 109, 535, 537
AspS9I GGNCC 2 cut(s) 293, 551
AsuC2I CCSGG 1 cut(s) 254
AsuHPI GGTGA 2 cut(s) 37, 107
AvaII GGWCC 2 cut(s) 293, 551
BanI GGYRCC 1 cut(s) 145
BclI TGATCA 2 cut(s) 42, 573
BcnI CCSGG 1 cut(s) 254
BcoDI GTCTC 1 cut(s) 449
BfaI CTAG 1 cut(s) 603
BfmI CTRYAG 2 cut(s) 539, 581
BmcAI AGTACT 1 cut(s) 113
Bme1390I CCNGG 1 cut(s) 254
Bme18I GGWCC 2 cut(s) 293, 551
BmgT120I GGNCC 2 cut(s) 293, 551
BmiI GGNNCC 2 cut(s) 147, 258
BmrFI CCNGG 1 cut(s) 254
BmsI GCATC 2 cut(s) 253, 436
BpuEI CTTGAG 1 cut(s) 342
BpuMI CCSGG 1 cut(s) 254
BsaBI GATNNNNATC 1 cut(s) 41
BsaXI ACNNNNNCTCC 2 cut(s) 605, 635
Bsc4I CCNNNNNNNGG 2 cut(s) 252, 608
Bse8I GATNNNNATC 1 cut(s) 41
BseJI GATNNNNATC 1 cut(s) 41
BseLI CCNNNNNNNGG 2 cut(s) 252, 608
BsePI GCGCGC 1 cut(s) 533
Bsh1236I CGCG 1 cut(s) 535
BshNI GGYRCC 1 cut(s) 145
BsiSI CCGG 2 cut(s) 253, 424
BslI CCNNNNNNNGG 2 cut(s) 252, 608
BsmAI GTCTC 1 cut(s) 449
Bsp143I GATC 4 cut(s) 42, 97, 573, 598
BspACI CCGC 1 cut(s) 609
BspFNI CGCG 1 cut(s) 535
BspLI GGNNCC 2 cut(s) 147, 258
BspMAI CTGCAG 1 cut(s) 585
BspPI GGATC 2 cut(s) 92, 593
BspT107I GGYRCC 1 cut(s) 145
BssHII GCGCGC 1 cut(s) 533
BssMI GATC 4 cut(s) 42, 97, 573, 598
Bst6I CTCTTC 1 cut(s) 70
BstC8I GCNNGC 1 cut(s) 535
BstFNI CGCG 1 cut(s) 535
BstHHI GCGC 3 cut(s) 109, 535, 537
BstKTI GATC 4 cut(s) 45, 100, 576, 601
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 4 cut(s) 42, 97, 573, 598
BstMWI GCNNNNNNNGC 1 cut(s) 319
BstSCI CCNGG 1 cut(s) 252
BstSFI CTRYAG 2 cut(s) 539, 581
BstUI CGCG 1 cut(s) 535
Cac8I GCNNGC 1 cut(s) 535
CfoI GCGC 3 cut(s) 109, 535, 537
Cfr13I GGNCC 2 cut(s) 293, 551
CseI GACGC 1 cut(s) 515
Csp6I GTAC 3 cut(s) 112, 146, 436
CviAII CATG 1 cut(s) 374
CviJI RGCY 6 cut(s) 72, 282, 313, 322, 467, 503
CviKI_1 RGCY 6 cut(s) 72, 282, 313, 322, 467, 503
CviQI GTAC 3 cut(s) 112, 146, 436
DpnI GATC 4 cut(s) 44, 99, 575, 600
DpnII GATC 4 cut(s) 42, 97, 573, 598
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Eco32I GATATC 1 cut(s) 340
Eco47I GGWCC 2 cut(s) 293, 551
Eco57I CTGAAG 2 cut(s) 489, 537
EcoRI GAATTC 1 cut(s) 218
EcoRV GATATC 1 cut(s) 340
FaeI CATG 1 cut(s) 377
FatI CATG 1 cut(s) 373
FauI CCCGC 1 cut(s) 602
FbaI TGATCA 2 cut(s) 42, 573
FspBI CTAG 1 cut(s) 603
FspI TGCGCA 1 cut(s) 108
GlaI GCGC 3 cut(s) 108, 534, 536
HapII CCGG 2 cut(s) 253, 424
HgaI GACGC 1 cut(s) 515
HhaI GCGC 3 cut(s) 109, 535, 537
Hin1II CATG 1 cut(s) 377
Hin6I GCGC 3 cut(s) 107, 533, 535
HinP1I GCGC 3 cut(s) 107, 533, 535
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 3 cut(s) 23, 28, 494
HpaII CCGG 2 cut(s) 253, 424
HphI GGTGA 2 cut(s) 37, 107
Hpy166II GTNNAC 1 cut(s) 628
Hpy188I TCNGA 1 cut(s) 33
Hpy188III TCNNGA 1 cut(s) 359
Hpy8I GTNNAC 1 cut(s) 628
HpyAV CCTTC 1 cut(s) 328
HpyCH4V TGCA 3 cut(s) 206, 266, 583
HpyF10VI GCNNNNNNNGC 1 cut(s) 319
Hsp92II CATG 1 cut(s) 377
HspAI GCGC 3 cut(s) 107, 533, 535
KpnI GGTACC 1 cut(s) 149
Ksp22I TGATCA 2 cut(s) 42, 573
Kzo9I GATC 4 cut(s) 42, 97, 573, 598
LpnPI CCDG 9 cut(s) 54, 266, 273, 416, 437, 449, 527, 569, 574
LweI GCATC 2 cut(s) 253, 436
MaeI CTAG 1 cut(s) 603
MaeIII GTNAC 2 cut(s) 345, 388
MalI GATC 4 cut(s) 44, 99, 575, 600
MboI GATC 4 cut(s) 42, 97, 573, 598
MboII GAAGA 3 cut(s) 46, 57, 412
MluCI AATT 8 cut(s) 168, 192, 218, 231, 273, 367, 569, 652
MnlI CCTC 4 cut(s) 74, 366, 564, 599
MseI TTAA 4 cut(s) 60, 230, 309, 567
MspI CCGG 2 cut(s) 253, 424
MspR9I CCNGG 1 cut(s) 254
MvnI CGCG 1 cut(s) 535
MwoI GCNNNNNNNGC 1 cut(s) 319
NciI CCSGG 1 cut(s) 254
NdeII GATC 4 cut(s) 42, 97, 573, 598
NlaIII CATG 1 cut(s) 377
NlaIV GGNNCC 2 cut(s) 147, 258
NmuCI GTSAC 1 cut(s) 345
NsbI TGCGCA 1 cut(s) 108
PauI GCGCGC 1 cut(s) 533
PfeI GAWTC 3 cut(s) 23, 28, 494
PspN4I GGNNCC 2 cut(s) 147, 258
PspPI GGNCC 2 cut(s) 293, 551
PstI CTGCAG 1 cut(s) 585
PteI GCGCGC 1 cut(s) 533
RsaI GTAC 3 cut(s) 113, 147, 437
RsaNI GTAC 3 cut(s) 112, 146, 436
SaqAI TTAA 4 cut(s) 60, 230, 309, 567
Sau3AI GATC 4 cut(s) 42, 97, 573, 598
Sau96I GGNCC 2 cut(s) 293, 551
ScaI AGTACT 1 cut(s) 113
ScrFI CCNGG 1 cut(s) 254
SetI ASST 7 cut(s) 147, 289, 298, 315, 324, 505, 636
SfaNI GCATC 2 cut(s) 253, 436
SfcI CTRYAG 2 cut(s) 539, 581
SinI GGWCC 2 cut(s) 293, 551
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
Sse9I AATT 8 cut(s) 168, 192, 218, 231, 273, 367, 569, 652
SsiI CCGC 1 cut(s) 609
SspMI CTAG 1 cut(s) 603
StyD4I CCNGG 1 cut(s) 252
TaqI TCGA 1 cut(s) 26
TasI AATT 8 cut(s) 168, 192, 218, 231, 273, 367, 569, 652
TatI WGTACW 1 cut(s) 111
TfiI GAWTC 3 cut(s) 23, 28, 494
Tru1I TTAA 4 cut(s) 60, 230, 309, 567
Tru9I TTAA 4 cut(s) 60, 230, 309, 567
TseFI GTSAC 1 cut(s) 345
Tsp45I GTSAC 1 cut(s) 345
TspDTI ATGAA 4 cut(s) 230, 362, 645, 664
TspGWI ACGGA 1 cut(s) 346
VpaK11BI GGWCC 2 cut(s) 293, 551
XapI RAATTY 2 cut(s) 218, 367
XspI CTAG 1 cut(s) 603
ZrmI AGTACT 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.