Rh1DG144800

Ribosome production factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0002361
Physical Location & Seq
Forward (+)
30503376 .. 30511577
8202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG144800.1

Sequence Viewer

Length: 885 bp
ATGACGATGAAGCCGCAGATGATCAGAGCCAATTCGAAGTTCAGGGAGAGACTGCAAGAAGTGCTGAAATGGTGCTCAATGAAGATGATTTTGAAGAGGGAAGTACTAATTGGGAAGAAGGATGAGATATATGGTGGCAGGTTATTAGCGAGTTTGCAGTTGTTTGTTAGAAATGATGCGGATGAGTTTAGTGCGGTTTTGAAGCGTGAATCGAATCCGAAGATATTGATCACCACTTGCCGATTTAACTCTTCTAGGGGTCCTTCTTTTATAGAGGAACTACTTTCGGTGATCCCAAATGCGCAGTACTATAAAAGAGGCACTTATGACTTGAAAAAGATTATAGAGTATGCAAATAAAAAGGAATTCACTTCTTTAATTGTTGTTCATAGCAATCGTCGGGAACCAGATGCTCTCCTAATTATTGGCTTACCTAATGGACCTACTGCCCATTTTAAGCTCTCGAAGCTTGTTTTACGGAAGGATATCAAGAATCATGGAAATCCAACCAGTCATGAGCCTGAGCTTGTATTAAACAACTTTACAACACGCCTTGGTCATCGTATTGGGAGATTAATACAATCACTTTTCCCTCAAGAACCAAATTTTCGTGGTCGGCGAGTTGTAACTTTCCACAACCAGCGAGATTTTATATTCTTCCGGCATCATCGGTACATTTTTGAAACCAAAGAGACTAAACAGGCTGAAGTAAAGGGCAAAAAGACCAAGAATTCCAAAGCTGACGCTATTACTGAAGCAAAACCATTTGCGCCCCTACAGGAATGTGGTCCTCGTTTTACCCTTAAATTGATCAGTCTGCAGCACGGGACATTTGATCCTAGAGGCGGGGAGTTTGAGTGGGTTCACAAGGATTTTGAATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Brix

Protein Analysis

294

Amino Acids

34.3

Weight (kDa)

9.95

Isoelectric Point (pI)

37.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Brix PF04427 83 - 271 1.1e-33 Brix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 303
Acc36I ACCTGC 1 cut(s) 129
AciI CCGC 4 cut(s) 14, 179, 194, 846
AclWI GGATC 2 cut(s) 286, 830
AcsI RAATTY 3 cut(s) 365, 604, 730
AcuI CTGAAG 2 cut(s) 726, 774
AfaI GTAC 3 cut(s) 105, 308, 674
AfiI CCNNNNNNNGG 1 cut(s) 845
AgsI TTSAA 5 cut(s) 94, 202, 334, 683, 878
AjuI GAANNNNNNNTTGG 4 cut(s) 23, 55, 93, 125
AluBI AGCT 4 cut(s) 460, 469, 526, 740
AluI AGCT 4 cut(s) 460, 469, 526, 740
Alw21I GWGCWC 1 cut(s) 77
Alw26I GTCTC 2 cut(s) 43, 686
AlwI GGATC 2 cut(s) 286, 830
ApeKI GCWGC 1 cut(s) 820
ApoI RAATTY 3 cut(s) 365, 604, 730
AseI ATTAAT 1 cut(s) 575
AspLEI GCGC 2 cut(s) 304, 772
AspS9I GGNCC 3 cut(s) 260, 440, 788
AsuHPI GGTGA 2 cut(s) 223, 301
AsuII TTCGAA 1 cut(s) 35
AvaII GGWCC 3 cut(s) 260, 440, 788
Bbv12I GWGCWC 1 cut(s) 77
BbvI GCAGC 1 cut(s) 832
BclI TGATCA 3 cut(s) 21, 228, 810
BcoDI GTCTC 2 cut(s) 43, 686
BfaI CTAG 2 cut(s) 255, 840
BfmI CTRYAG 2 cut(s) 776, 818
BfuAI ACCTGC 1 cut(s) 129
BisI GCNGC 2 cut(s) 14, 821
BlsI GCNGC 2 cut(s) 15, 822
BmcAI AGTACT 2 cut(s) 105, 308
Bme18I GGWCC 3 cut(s) 260, 440, 788
BmgT120I GGNCC 3 cut(s) 260, 440, 788
BmiI GGNNCC 2 cut(s) 261, 405
BmsI GCATC 3 cut(s) 166, 400, 673
Bpu10I CCTNAGC 1 cut(s) 522
Bpu14I TTCGAA 1 cut(s) 35
BpuEI CTTGAG 1 cut(s) 579
BsaBI GATNNNNATC 1 cut(s) 227
BsaJI CCNNGG 1 cut(s) 553
BsaXI ACNNNNNCTCC 2 cut(s) 842, 872
Bsc4I CCNNNNNNNGG 1 cut(s) 845
Bse1I ACTGG 1 cut(s) 510
Bse8I GATNNNNATC 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 553
BseGI GGATG 2 cut(s) 127, 187
BseJI GATNNNNATC 1 cut(s) 227
BseLI CCNNNNNNNGG 1 cut(s) 845
BseMII CTCAG 1 cut(s) 513
BseNI ACTGG 1 cut(s) 510
BseXI GCAGC 1 cut(s) 832
BsiHKAI GWGCWC 1 cut(s) 77
BsiSI CCGG 1 cut(s) 661
BslFI GGGAC 1 cut(s) 841
BslI CCNNNNNNNGG 1 cut(s) 845
BsmAI GTCTC 2 cut(s) 43, 686
BsmFI GGGAC 1 cut(s) 841
Bsp119I TTCGAA 1 cut(s) 35
Bsp1286I GDGCHC 1 cut(s) 77
Bsp143I GATC 5 cut(s) 21, 228, 291, 810, 835
BspACI CCGC 4 cut(s) 14, 179, 194, 846
BspCNI CTCAG 1 cut(s) 514
BspHI TCATGA 1 cut(s) 514
BspLI GGNNCC 2 cut(s) 261, 405
BspMAI CTGCAG 1 cut(s) 822
BspMI ACCTGC 1 cut(s) 129
BspPI GGATC 2 cut(s) 286, 830
BspT104I TTCGAA 1 cut(s) 35
BsrI ACTGG 1 cut(s) 510
BssECI CCNNGG 1 cut(s) 553
BssMI GATC 5 cut(s) 21, 228, 291, 810, 835
BssT1I CCWWGG 1 cut(s) 553
Bst6I CTCTTC 2 cut(s) 89, 256
BstAPI GCANNNNNTGC 1 cut(s) 61
BstBI TTCGAA 1 cut(s) 35
BstDEI CTNAG 1 cut(s) 522
BstF5I GGATG 2 cut(s) 127, 187
BstHHI GCGC 2 cut(s) 304, 772
BstKTI GATC 5 cut(s) 24, 231, 294, 813, 838
BstMAI GTCTC 2 cut(s) 43, 686
BstMBI GATC 5 cut(s) 21, 228, 291, 810, 835
BstMWI GCNNNNNNNGC 2 cut(s) 61, 466
BstSFI CTRYAG 2 cut(s) 776, 818
BstV1I GCAGC 1 cut(s) 832
BtsCI GGATG 2 cut(s) 127, 187
BveI ACCTGC 1 cut(s) 129
CciI TCATGA 1 cut(s) 514
CfoI GCGC 2 cut(s) 304, 772
Cfr13I GGNCC 3 cut(s) 260, 440, 788
CseI GACGC 1 cut(s) 752
Csp6I GTAC 3 cut(s) 104, 307, 673
CviAII CATG 2 cut(s) 497, 515
CviJI RGCY 9 cut(s) 13, 29, 429, 460, 469, 520, 526, 704, 740
CviKI_1 RGCY 9 cut(s) 13, 29, 429, 460, 469, 520, 526, 704, 740
CviQI GTAC 3 cut(s) 104, 307, 673
DdeI CTNAG 1 cut(s) 522
DpnI GATC 5 cut(s) 23, 230, 293, 812, 837
DpnII GATC 5 cut(s) 21, 228, 291, 810, 835
Eam1104I CTCTTC 2 cut(s) 89, 256
EarI CTCTTC 2 cut(s) 89, 256
Eco130I CCWWGG 1 cut(s) 553
Eco32I GATATC 1 cut(s) 487
Eco47I GGWCC 3 cut(s) 260, 440, 788
Eco57I CTGAAG 2 cut(s) 726, 774
EcoO109I RGGNCCY 1 cut(s) 260
EcoRI GAATTC 2 cut(s) 365, 730
EcoRV GATATC 1 cut(s) 487
EcoT14I CCWWGG 1 cut(s) 553
ErhI CCWWGG 1 cut(s) 553
FaeI CATG 2 cut(s) 500, 518
FalI AAGNNNNNCTT 2 cut(s) 307, 339
FaqI GGGAC 1 cut(s) 841
FatI CATG 2 cut(s) 496, 514
FauI CCCGC 1 cut(s) 839
FbaI TGATCA 3 cut(s) 21, 228, 810
Fnu4HI GCNGC 2 cut(s) 14, 821
FokI GGATG 2 cut(s) 134, 194
Fsp4HI GCNGC 2 cut(s) 14, 821
FspBI CTAG 2 cut(s) 255, 840
FspI TGCGCA 1 cut(s) 303
GlaI GCGC 2 cut(s) 303, 771
GluI GCNGC 2 cut(s) 14, 821
HapII CCGG 1 cut(s) 661
HgaI GACGC 1 cut(s) 752
HhaI GCGC 2 cut(s) 304, 772
Hin1II CATG 2 cut(s) 500, 518
Hin6I GCGC 2 cut(s) 302, 770
HinP1I GCGC 2 cut(s) 302, 770
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 3 cut(s) 209, 214, 493
HpaII CCGG 1 cut(s) 661
HphI GGTGA 2 cut(s) 223, 301
Hpy166II GTNNAC 1 cut(s) 865
Hpy188I TCNGA 2 cut(s) 26, 219
Hpy188III TCNNGA 5 cut(s) 401, 463, 490, 515, 596
Hpy8I GTNNAC 1 cut(s) 865
Hpy99I CGWCG 1 cut(s) 402
HpyAV CCTTC 3 cut(s) 112, 273, 475
HpyCH4V TGCA 4 cut(s) 55, 157, 353, 820
HpyF10VI GCNNNNNNNGC 2 cut(s) 61, 466
HpyF3I CTNAG 1 cut(s) 522
Hsp92II CATG 2 cut(s) 500, 518
HspAI GCGC 2 cut(s) 302, 770
Ksp22I TGATCA 3 cut(s) 21, 228, 810
Kzo9I GATC 5 cut(s) 21, 228, 291, 810, 835
LpnPI CCDG 9 cut(s) 28, 124, 420, 523, 534, 653, 674, 686, 764
Lsp1109I GCAGC 1 cut(s) 832
LweI GCATC 3 cut(s) 166, 400, 673
MaeI CTAG 2 cut(s) 255, 840
MaeIII GTNAC 1 cut(s) 625
MalI GATC 5 cut(s) 23, 230, 293, 812, 837
MboI GATC 5 cut(s) 21, 228, 291, 810, 835
MboII GAAGA 6 cut(s) 94, 106, 127, 232, 243, 649
MhlI GDGCHC 1 cut(s) 77
MluCI AATT 8 cut(s) 31, 108, 365, 378, 420, 604, 730, 806
MmeI TCCRAC 1 cut(s) 530
MnlI CCTC 6 cut(s) 90, 268, 311, 603, 801, 836
MseI TTAA 6 cut(s) 246, 377, 456, 533, 575, 804
MspI CCGG 1 cut(s) 661
MwoI GCNNNNNNNGC 2 cut(s) 61, 466
NdeII GATC 5 cut(s) 21, 228, 291, 810, 835
NlaIII CATG 2 cut(s) 500, 518
NlaIV GGNNCC 2 cut(s) 261, 405
NsbI TGCGCA 1 cut(s) 303
NspV TTCGAA 1 cut(s) 35
PagI TCATGA 1 cut(s) 514
PcsI WCGNNNNNNNCGW 1 cut(s) 616
PfeI GAWTC 3 cut(s) 209, 214, 493
PkrI GCNGC 2 cut(s) 15, 822
PpuMI RGGWCCY 1 cut(s) 260
PshBI ATTAAT 1 cut(s) 575
Psp5II RGGWCCY 1 cut(s) 260
PspN4I GGNNCC 2 cut(s) 261, 405
PspPI GGNCC 3 cut(s) 260, 440, 788
PspPPI RGGWCCY 1 cut(s) 260
PstI CTGCAG 1 cut(s) 822
RsaI GTAC 3 cut(s) 105, 308, 674
RsaNI GTAC 3 cut(s) 104, 307, 673
SaqAI TTAA 6 cut(s) 246, 377, 456, 533, 575, 804
SatI GCNGC 2 cut(s) 14, 821
Sau3AI GATC 5 cut(s) 21, 228, 291, 810, 835
Sau96I GGNCC 3 cut(s) 260, 440, 788
ScaI AGTACT 2 cut(s) 105, 308
SduI GDGCHC 1 cut(s) 77
SetI ASST 7 cut(s) 143, 436, 445, 462, 471, 528, 742
SfaNI GCATC 3 cut(s) 166, 400, 673
SfcI CTRYAG 2 cut(s) 776, 818
SfuI TTCGAA 1 cut(s) 35
SinI GGWCC 3 cut(s) 260, 440, 788
SmlI CTYRAG 1 cut(s) 594
SmoI CTYRAG 1 cut(s) 594
Sse9I AATT 8 cut(s) 31, 108, 365, 378, 420, 604, 730, 806
SsiI CCGC 4 cut(s) 14, 179, 194, 846
SspMI CTAG 2 cut(s) 255, 840
StyI CCWWGG 1 cut(s) 553
TaqI TCGA 3 cut(s) 35, 212, 464
TasI AATT 8 cut(s) 31, 108, 365, 378, 420, 604, 730, 806
TatI WGTACW 2 cut(s) 103, 306
TauI GCSGC 1 cut(s) 16
TfiI GAWTC 3 cut(s) 209, 214, 493
Tru1I TTAA 6 cut(s) 246, 377, 456, 533, 575, 804
Tru9I TTAA 6 cut(s) 246, 377, 456, 533, 575, 804
TseI GCWGC 1 cut(s) 820
TspDTI ATGAA 3 cut(s) 23, 95, 377
TspGWI ACGGA 1 cut(s) 493
VpaK11BI GGWCC 3 cut(s) 260, 440, 788
VspI ATTAAT 1 cut(s) 575
XapI RAATTY 3 cut(s) 365, 604, 730
XspI CTAG 2 cut(s) 255, 840
ZrmI AGTACT 2 cut(s) 105, 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.