MD07G1164400.v1.1

Ribosome production factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002361
Physical Location & Seq
Reverse (-)
23904107 .. 23908285
4179 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1164400.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGGGAGGCGATAACAATAAAAGGAAGCGAAGCGAAAGCGAAGGAGGAGGAGGAGAAGAACCGCCGCCGGAGAAGAAGATGGAGATTCTGCCGTCGATGATAAAGAACAAGGAGAAGAGGTCGGCGGTGCACGCCAAGCTCAAGCAGCAGAAGAAGCTCGAGAAGCGCAAGAAGGCCAAAGCGCGCGACGCCGCCGAGAAGCGCGCTCTCGAGCTCGGCGAGGAGCCGCCGGCGAAGAAGATTCCCAAGACGATTGAGAACACCAGGGAAGTCGATGAGACTATTTGTAAGCCTGACGACGAAGAGCTGTTTGCTGGAAATGATGCCGATGAATTCAGTTCGATTTTAAAGCGCGAAATGAATCCAAAGATATTAATCACTACTTGCCGTTTCCATTCTTCTAGGGGGCCTGAGTTTATAAAGGAACTACTTTCAGTGATCCCAAATGCAACCTACTACAAAAGAGGAACATATGACTTGAAAAAGATTGTAGAGTACGCAAATAAGAAGGACTTCACTTCTCTAATTGTTGTTCACACCAATCGCAGAGAACCAGATGCTCTCCTAATAATTGGCCTACCTTCCGGGCCTACTGCCCATTTTAAGCTCTCAAAGCTTGTTTTACGCAAGGATATCAAGAATCATGGAAATCCAACCAGTCATGAGCCCGAGCTTGTGCTGAACAACTTTACAACACGCCTTGGTCATCGAATTGGAAGATTAATTCAGTCACTTTTCCCACAAGAACCAAATTTTCGTGGTCGGCGAGTTGTAACTTTTCACAACCAGCGAGATTTTATATTCTTCCGGCATCATCGGTATATCTTTGAAGAAAAAGAGGCTAAACAGATCGAGTCCAAAGGTAAAAAGACCAAGGATGCAAATGGTGAGAGCGGACCTAAAGGGAAAGTAATTGCTCGCCTACAGGAGTGTGGCCCTCGTTTCACCCTTAAACTAATTAGTCTGCAGCATGGGACCTTCGATACTAAAGGCGGGGAATATGAGTGGGTTCACAAGCCGGAAATGGACACAAGCAGAAGGAGATTTTTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Brix

Protein Analysis

352

Amino Acids

40.38

Weight (kDa)

9.74

Isoelectric Point (pI)

49.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Brix PF04427 133 - 320 6.3e-34 Brix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 419
AccBSI CCGCTC 1 cut(s) 894
AccII CGCG 4 cut(s) 184, 186, 204, 354
AciI CCGC 7 cut(s) 62, 65, 125, 192, 227, 894, 993
AclWI GGATC 1 cut(s) 433
AcsI RAATTY 2 cut(s) 332, 751
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 1 cut(s) 497
AgsI TTSAA 2 cut(s) 481, 830
AjnI CCWGG 1 cut(s) 263
AluBI AGCT 7 cut(s) 139, 157, 214, 307, 607, 616, 673
AluI AGCT 7 cut(s) 139, 157, 214, 307, 607, 616, 673
Alw21I GWGCWC 2 cut(s) 132, 216
Alw26I GTCTC 1 cut(s) 272
Alw44I GTGCAC 1 cut(s) 128
AlwI GGATC 1 cut(s) 433
Ama87I CYCGRG 3 cut(s) 158, 209, 668
AoxI GGCC 5 cut(s) 174, 407, 574, 587, 934
ApaLI GTGCAC 1 cut(s) 128
ApeKI GCWGC 2 cut(s) 145, 967
ApoI RAATTY 2 cut(s) 332, 751
AseI ATTAAT 2 cut(s) 374, 722
Asp700I GAANNNNTTC 1 cut(s) 512
AspLEI GCGC 6 cut(s) 168, 184, 186, 204, 206, 354
AspS9I GGNCC 5 cut(s) 407, 587, 896, 935, 975
AsuC2I CCSGG 1 cut(s) 586
AsuHPI GGTGA 2 cut(s) 899, 937
AvaI CYCGRG 3 cut(s) 158, 209, 668
AvaII GGWCC 2 cut(s) 896, 975
BaeGI GKGCMC 1 cut(s) 132
BanII GRGCYC 2 cut(s) 216, 669
Bbv12I GWGCWC 2 cut(s) 132, 216
BbvI GCAGC 2 cut(s) 157, 979
BccI CCATC 1 cut(s) 73
BceAI ACGGC 2 cut(s) 76, 372
BciT130I CCWGG 1 cut(s) 265
BcnI CCSGG 1 cut(s) 586
BcoDI GTCTC 1 cut(s) 272
BfaI CTAG 1 cut(s) 402
BfmI CTRYAG 2 cut(s) 923, 965
BisI GCNGC 5 cut(s) 65, 146, 192, 227, 968
BlsI GCNGC 5 cut(s) 66, 147, 193, 228, 969
Bme1390I CCNGG 2 cut(s) 265, 586
Bme18I GGWCC 2 cut(s) 896, 975
BmeT110I CYCGRG 3 cut(s) 158, 209, 668
BmgT120I GGNCC 5 cut(s) 407, 587, 896, 935, 975
BmiI GGNNCC 3 cut(s) 225, 408, 976
BmrFI CCNGG 2 cut(s) 265, 586
BmsI GCATC 4 cut(s) 313, 547, 820, 868
BpuEI CTTGAG 1 cut(s) 125
BpuMI CCSGG 1 cut(s) 586
BsaBI GATNNNNATC 1 cut(s) 374
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 3 cut(s) 264, 700, 873
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bse118I RCCGGY 1 cut(s) 229
Bse1I ACTGG 1 cut(s) 657
Bse8I GATNNNNATC 1 cut(s) 374
BseBI CCWGG 1 cut(s) 265
BseDI CCNNGG 3 cut(s) 264, 700, 873
BseGI GGATG 1 cut(s) 883
BseJI GATNNNNATC 1 cut(s) 374
BseMII CTCAG 1 cut(s) 402
BseNI ACTGG 1 cut(s) 657
BsePI GCGCGC 2 cut(s) 182, 202
BseRI GAGGAG 4 cut(s) 60, 63, 66, 236
BseSI GKGCMC 1 cut(s) 132
BseXI GCAGC 2 cut(s) 157, 979
Bsh1236I CGCG 4 cut(s) 184, 186, 204, 354
BshFI GGCC 5 cut(s) 176, 409, 576, 589, 936
BsiHKAI GWGCWC 2 cut(s) 132, 216
BsiHKCI CYCGRG 3 cut(s) 158, 209, 668
BsiSI CCGG 5 cut(s) 68, 230, 585, 808, 1019
BslFI GGGAC 1 cut(s) 988
BsmAI GTCTC 1 cut(s) 272
BsmFI GGGAC 1 cut(s) 988
BsnI GGCC 5 cut(s) 176, 409, 576, 589, 936
BsoBI CYCGRG 3 cut(s) 158, 209, 668
Bsp1286I GDGCHC 3 cut(s) 132, 216, 669
Bsp143I GATC 2 cut(s) 438, 849
BspACI CCGC 7 cut(s) 62, 65, 125, 192, 227, 894, 993
BspANI GGCC 5 cut(s) 176, 409, 576, 589, 936
BspCNI CTCAG 1 cut(s) 403
BspFNI CGCG 4 cut(s) 184, 186, 204, 354
BspHI TCATGA 1 cut(s) 661
BspLI GGNNCC 3 cut(s) 225, 408, 976
BspMAI CTGCAG 1 cut(s) 969
BspPI GGATC 1 cut(s) 433
BspQI GCTCTTC 1 cut(s) 297
BsrBI CCGCTC 1 cut(s) 894
BsrFI RCCGGY 1 cut(s) 229
BsrI ACTGG 1 cut(s) 657
BssAI RCCGGY 1 cut(s) 229
BssECI CCNNGG 3 cut(s) 264, 700, 873
BssHII GCGCGC 2 cut(s) 182, 202
BssMI GATC 2 cut(s) 438, 849
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 2 cut(s) 700, 873
Bst2UI CCWGG 1 cut(s) 265
Bst6I CTCTTC 2 cut(s) 110, 297
BstACI GRCGYC 1 cut(s) 189
BstC8I GCNNGC 5 cut(s) 132, 184, 204, 231, 919
BstDEI CTNAG 1 cut(s) 411
BstF5I GGATG 1 cut(s) 883
BstFNI CGCG 4 cut(s) 184, 186, 204, 354
BstHHI GCGC 6 cut(s) 168, 184, 186, 204, 206, 354
BstKTI GATC 2 cut(s) 441, 852
BstMAI GTCTC 1 cut(s) 272
BstMBI GATC 2 cut(s) 438, 849
BstMWI GCNNNNNNNGC 7 cut(s) 131, 136, 145, 154, 163, 188, 613
BstNI CCWGG 1 cut(s) 265
BstSCI CCNGG 2 cut(s) 263, 584
BstSFI CTRYAG 2 cut(s) 923, 965
BstSLI GKGCMC 1 cut(s) 132
BstUI CGCG 4 cut(s) 184, 186, 204, 354
BstV1I GCAGC 2 cut(s) 157, 979
BsuRI GGCC 5 cut(s) 176, 409, 576, 589, 936
BtsCI GGATG 1 cut(s) 883
BtsIMutI CAGTG 1 cut(s) 441
Cac8I GCNNGC 5 cut(s) 132, 184, 204, 231, 919
CciI TCATGA 1 cut(s) 661
CfoI GCGC 6 cut(s) 168, 184, 186, 204, 206, 354
Cfr10I RCCGGY 1 cut(s) 229
Cfr13I GGNCC 5 cut(s) 407, 587, 896, 935, 975
CseI GACGC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 496
CviAII CATG 3 cut(s) 644, 662, 971
CviQI GTAC 1 cut(s) 496
DdeI CTNAG 1 cut(s) 411
DpnI GATC 2 cut(s) 440, 851
DpnII GATC 2 cut(s) 438, 849
DraI TTTAAA 1 cut(s) 348
Eam1104I CTCTTC 2 cut(s) 110, 297
EarI CTCTTC 2 cut(s) 110, 297
Ecl136II GAGCTC 1 cut(s) 214
Eco130I CCWWGG 2 cut(s) 700, 873
Eco24I GRGCYC 2 cut(s) 216, 669
Eco32I GATATC 1 cut(s) 634
Eco47I GGWCC 2 cut(s) 896, 975
Eco53kI GAGCTC 1 cut(s) 214
Eco88I CYCGRG 3 cut(s) 158, 209, 668
EcoICRI GAGCTC 1 cut(s) 214
EcoO109I RGGNCCY 2 cut(s) 407, 975
EcoRI GAATTC 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 263
EcoRV GATATC 1 cut(s) 634
EcoT14I CCWWGG 2 cut(s) 700, 873
EcoT38I GRGCYC 2 cut(s) 216, 669
ErhI CCWWGG 2 cut(s) 700, 873
FaeI CATG 3 cut(s) 647, 665, 974
FaiI YATR 9 cut(s) 419, 472, 474, 645, 663, 800, 822, 972, 1002
FalI AAGNNNNNCTT 2 cut(s) 497, 529
FaqI GGGAC 1 cut(s) 988
FatI CATG 3 cut(s) 643, 661, 970
FauI CCCGC 1 cut(s) 986
FauNDI CATATG 1 cut(s) 472
Fnu4HI GCNGC 5 cut(s) 65, 146, 192, 227, 968
FokI GGATG 1 cut(s) 890
FriOI GRGCYC 2 cut(s) 216, 669
Fsp4HI GCNGC 5 cut(s) 65, 146, 192, 227, 968
FspBI CTAG 1 cut(s) 402
GlaI GCGC 6 cut(s) 167, 183, 185, 203, 205, 353
GluI GCNGC 5 cut(s) 65, 146, 192, 227, 968
HaeIII GGCC 5 cut(s) 176, 409, 576, 589, 936
HapII CCGG 5 cut(s) 68, 230, 585, 808, 1019
HgaI GACGC 1 cut(s) 197
HhaI GCGC 6 cut(s) 168, 184, 186, 204, 206, 354
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 3 cut(s) 647, 665, 974
Hin6I GCGC 6 cut(s) 166, 182, 184, 202, 204, 352
HinP1I GCGC 6 cut(s) 166, 182, 184, 202, 204, 352
HindIII AAGCTT 1 cut(s) 614
HinfI GANTC 5 cut(s) 85, 241, 361, 640, 854
HpaII CCGG 5 cut(s) 68, 230, 585, 808, 1019
HphI GGTGA 2 cut(s) 899, 937
Hpy166II GTNNAC 3 cut(s) 130, 535, 1012
Hpy188III TCNNGA 4 cut(s) 160, 209, 637, 662
Hpy8I GTNNAC 3 cut(s) 130, 535, 1012
Hpy99I CGWCG 3 cut(s) 97, 191, 302
HpyAV CCTTC 6 cut(s) 35, 166, 502, 591, 988, 1032
HpyCH4V TGCA 4 cut(s) 130, 449, 881, 967
HpyF10VI GCNNNNNNNGC 7 cut(s) 131, 136, 145, 154, 163, 188, 613
HpyF3I CTNAG 1 cut(s) 411
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 3 cut(s) 647, 665, 974
HspAI GCGC 6 cut(s) 166, 182, 184, 202, 204, 352
KroI GCCGGC 1 cut(s) 229
KroNI GCCGGC 1 cut(s) 231
Kzo9I GATC 2 cut(s) 438, 849
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 2 cut(s) 157, 979
LweI GCATC 4 cut(s) 313, 547, 820, 868
MaeI CTAG 1 cut(s) 402
MaeIII GTNAC 2 cut(s) 729, 772
MalI GATC 2 cut(s) 440, 851
MbiI CCGCTC 1 cut(s) 894
MboI GATC 2 cut(s) 438, 849
MhlI GDGCHC 3 cut(s) 132, 216, 669
MluCI AATT 8 cut(s) 332, 525, 570, 711, 723, 751, 912, 957
MlyI GAGTC 1 cut(s) 863
MmeI TCCRAC 1 cut(s) 677
MnlI CCTC 8 cut(s) 38, 41, 44, 111, 214, 458, 832, 948
MreI CGCCGGCG 1 cut(s) 229
MroNI GCCGGC 1 cut(s) 229
MroXI GAANNNNTTC 1 cut(s) 512
MseI TTAA 5 cut(s) 347, 374, 603, 722, 951
MspI CCGG 5 cut(s) 68, 230, 585, 808, 1019
MspR9I CCNGG 2 cut(s) 265, 586
MvaI CCWGG 1 cut(s) 265
MvnI CGCG 4 cut(s) 184, 186, 204, 354
MwoI GCNNNNNNNGC 7 cut(s) 131, 136, 145, 154, 163, 188, 613
NaeI GCCGGC 1 cut(s) 231
NciI CCSGG 1 cut(s) 586
NdeI CATATG 1 cut(s) 472
NdeII GATC 2 cut(s) 438, 849
NgoMIV GCCGGC 1 cut(s) 229
NlaIII CATG 3 cut(s) 647, 665, 974
NlaIV GGNNCC 3 cut(s) 225, 408, 976
NmeAIII GCCGAG 2 cut(s) 195, 220
NmuCI GTSAC 1 cut(s) 729
PaeR7I CTCGAG 2 cut(s) 158, 209
PagI TCATGA 1 cut(s) 661
PauI GCGCGC 2 cut(s) 182, 202
PciSI GCTCTTC 1 cut(s) 297
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 763
PdiI GCCGGC 1 cut(s) 231
PdmI GAANNNNTTC 1 cut(s) 512
PfeI GAWTC 4 cut(s) 85, 241, 361, 640
PkrI GCNGC 5 cut(s) 66, 147, 193, 228, 969
PleI GAGTC 1 cut(s) 862
PpsI GAGTC 1 cut(s) 862
PpuMI RGGWCCY 1 cut(s) 975
PshBI ATTAAT 2 cut(s) 374, 722
PsiI TTATAA 1 cut(s) 419
Psp124BI GAGCTC 1 cut(s) 216
Psp5II RGGWCCY 1 cut(s) 975
Psp6I CCWGG 1 cut(s) 263
PspGI CCWGG 1 cut(s) 263
PspN4I GGNNCC 3 cut(s) 225, 408, 976
PspPI GGNCC 5 cut(s) 407, 587, 896, 935, 975
PspPPI RGGWCCY 1 cut(s) 975
PstI CTGCAG 1 cut(s) 969
PteI GCGCGC 2 cut(s) 182, 202
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
SacI GAGCTC 1 cut(s) 216
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 5 cut(s) 347, 374, 603, 722, 951
SatI GCNGC 5 cut(s) 65, 146, 192, 227, 968
Sau3AI GATC 2 cut(s) 438, 849
Sau96I GGNCC 5 cut(s) 407, 587, 896, 935, 975
SchI GAGTC 1 cut(s) 863
ScrFI CCNGG 2 cut(s) 265, 586
SduI GDGCHC 3 cut(s) 132, 216, 669
SfaNI GCATC 4 cut(s) 313, 547, 820, 868
SfcI CTRYAG 2 cut(s) 923, 965
Sfr274I CTCGAG 2 cut(s) 158, 209
SgrAI CRCCGGYG 1 cut(s) 229
SinI GGWCC 2 cut(s) 896, 975
SlaI CTCGAG 2 cut(s) 158, 209
SmlI CTYRAG 3 cut(s) 140, 158, 209
SmoI CTYRAG 3 cut(s) 140, 158, 209
Sse9I AATT 8 cut(s) 332, 525, 570, 711, 723, 751, 912, 957
SsiI CCGC 7 cut(s) 62, 65, 125, 192, 227, 894, 993
SspMI CTAG 1 cut(s) 402
SstI GAGCTC 1 cut(s) 216
StyD4I CCNGG 2 cut(s) 263, 584
StyI CCWWGG 2 cut(s) 700, 873
TaqI TCGA 8 cut(s) 95, 159, 210, 273, 341, 709, 852, 981
TasI AATT 8 cut(s) 332, 525, 570, 711, 723, 751, 912, 957
TauI GCSGC 3 cut(s) 67, 194, 229
TfiI GAWTC 4 cut(s) 85, 241, 361, 640
Tru1I TTAA 5 cut(s) 347, 374, 603, 722, 951
Tru9I TTAA 5 cut(s) 347, 374, 603, 722, 951
TscAI CASTG 1 cut(s) 441
TseFI GTSAC 1 cut(s) 729
TseI GCWGC 2 cut(s) 145, 967
Tsp45I GTSAC 1 cut(s) 729
TspDTI ATGAA 2 cut(s) 345, 374
TspRI CASTG 1 cut(s) 441
VneI GTGCAC 1 cut(s) 128
VpaK11BI GGWCC 2 cut(s) 896, 975
VspI ATTAAT 2 cut(s) 374, 722
XapI RAATTY 2 cut(s) 332, 751
XhoI CTCGAG 2 cut(s) 158, 209
XmnI GAANNNNTTC 1 cut(s) 512
XspI CTAG 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.