RchiOBHm_Chr5g0055421

Ribosome production factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002361
Physical Location & Seq
Forward (+)
58465509 .. 58467268
1760 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33243

Sequence Viewer

Length: 612 bp
ATGTGTGCTAAGGTTTATGCTTTTTGTTGTTTTGGTGGTTTTCAGAGGGGTCCTGCTTTTATAGAGGAACTACTTTCGGTGATCCCAAATGCGCAGTACTATAAAAGAGACACTTATGACTTGAAAAATTTTCAAGTGGTTTCTTATGTAAGGATGGATTACCTTCAAGCTCCTAATGGTTTTCTTCTTTCACCTACTTTCTTCAGATTAATACAGTCACTTTTCCCTCAAGAACCAAATTTTAGTGGTCGGCGAGTTGTAACTTTCCACAACCAGCGAGATTTTATATTCTTCCGGCATCATCGATGTGCCTCCTTTGCTATTTTTAGTTCTTGCAGTGATCCTTTCTTATACTTGTACATTTTTGAAACCAAAGAGACTAAACAGGCTGAAGTAAAGGGCAAAAACACCAAGGATTCCAAAGTTGACACTATTACTGAAGCAAAACCATTTGCGCGCCTACAGGAATGTGGTCCTCGTTTTACCCTTAAATTGATCAGTCTGCAGCACGGGACATTTGATCCTAGAGGCGGGGAGTTTGAGTGGGTTCACAAGGTAATCCACTTATCAGTCAATTCATTTCAGTATATTGGTTTCATATCTTTATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Brix

Protein Analysis

203

Amino Acids

23.61

Weight (kDa)

9.01

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Brix PF04427 69 - 166 6.6e-11 Brix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 93
AccII CGCG 1 cut(s) 457
AciI CCGC 1 cut(s) 531
AclWI GGATC 3 cut(s) 76, 335, 515
AcsI RAATTY 2 cut(s) 127, 238
AcuI CTGAAG 3 cut(s) 187, 411, 459
AfaI GTAC 2 cut(s) 98, 359
AfiI CCNNNNNNNGG 1 cut(s) 530
AgsI TTSAA 4 cut(s) 124, 134, 167, 368
AluBI AGCT 1 cut(s) 170
AluI AGCT 1 cut(s) 170
Alw26I GTCTC 2 cut(s) 102, 371
AlwI GGATC 3 cut(s) 76, 335, 515
ApeKI GCWGC 1 cut(s) 505
ApoI RAATTY 2 cut(s) 127, 238
AseI ATTAAT 1 cut(s) 209
AspLEI GCGC 3 cut(s) 94, 457, 459
AspS9I GGNCC 2 cut(s) 50, 473
AsuHPI GGTGA 2 cut(s) 91, 183
AvaII GGWCC 2 cut(s) 50, 473
BbvI GCAGC 1 cut(s) 517
BccI CCATC 1 cut(s) 148
BclI TGATCA 1 cut(s) 495
BcoDI GTCTC 2 cut(s) 102, 371
BfaI CTAG 1 cut(s) 525
BfmI CTRYAG 2 cut(s) 461, 503
BisI GCNGC 1 cut(s) 506
BlsI GCNGC 1 cut(s) 507
BmcAI AGTACT 1 cut(s) 98
Bme18I GGWCC 2 cut(s) 50, 473
BmgT120I GGNCC 2 cut(s) 50, 473
BmiI GGNNCC 1 cut(s) 51
BmsI GCATC 1 cut(s) 307
Bpu10I CCTNAGC 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 213
Bsa29I ATCGAT 1 cut(s) 304
BsaJI CCNNGG 1 cut(s) 411
BsaXI ACNNNNNCTCC 2 cut(s) 527, 557
Bsc4I CCNNNNNNNGG 1 cut(s) 530
BseCI ATCGAT 1 cut(s) 304
BseDI CCNNGG 1 cut(s) 411
BseGI GGATG 1 cut(s) 159
BseLI CCNNNNNNNGG 1 cut(s) 530
BsePI GCGCGC 1 cut(s) 455
BseXI GCAGC 1 cut(s) 517
Bsh1236I CGCG 1 cut(s) 457
BshVI ATCGAT 1 cut(s) 304
BsiSI CCGG 1 cut(s) 295
BslFI GGGAC 1 cut(s) 526
BslI CCNNNNNNNGG 1 cut(s) 530
BsmAI GTCTC 2 cut(s) 102, 371
BsmFI GGGAC 1 cut(s) 526
Bsp1407I TGTACA 1 cut(s) 357
Bsp143I GATC 4 cut(s) 81, 340, 495, 520
BspACI CCGC 1 cut(s) 531
BspDI ATCGAT 1 cut(s) 304
BspFNI CGCG 1 cut(s) 457
BspLI GGNNCC 1 cut(s) 51
BspMAI CTGCAG 1 cut(s) 507
BspPI GGATC 3 cut(s) 76, 335, 515
BsrGI TGTACA 1 cut(s) 357
BssECI CCNNGG 1 cut(s) 411
BssHII GCGCGC 1 cut(s) 455
BssMI GATC 4 cut(s) 81, 340, 495, 520
BssT1I CCWWGG 1 cut(s) 411
Bst4CI ACNGT 1 cut(s) 216
BstAUI TGTACA 1 cut(s) 357
BstC8I GCNNGC 1 cut(s) 457
BstDEI CTNAG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 159
BstFNI CGCG 1 cut(s) 457
BstHHI GCGC 3 cut(s) 94, 457, 459
BstKTI GATC 4 cut(s) 84, 343, 498, 523
BstMAI GTCTC 2 cut(s) 102, 371
BstMBI GATC 4 cut(s) 81, 340, 495, 520
BstMWI GCNNNNNNNGC 1 cut(s) 317
BstSFI CTRYAG 2 cut(s) 461, 503
BstUI CGCG 1 cut(s) 457
BstV1I GCAGC 1 cut(s) 517
Bsu15I ATCGAT 1 cut(s) 304
BsuTUI ATCGAT 1 cut(s) 304
BtsCI GGATG 1 cut(s) 159
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 1 cut(s) 343
Cac8I GCNNGC 1 cut(s) 457
CfoI GCGC 3 cut(s) 94, 457, 459
Cfr13I GGNCC 2 cut(s) 50, 473
ClaI ATCGAT 1 cut(s) 304
Csp6I GTAC 2 cut(s) 97, 358
CviJI RGCY 2 cut(s) 170, 389
CviKI_1 RGCY 2 cut(s) 170, 389
CviQI GTAC 2 cut(s) 97, 358
DdeI CTNAG 1 cut(s) 9
DpnI GATC 4 cut(s) 83, 342, 497, 522
DpnII GATC 4 cut(s) 81, 340, 495, 520
Eco130I CCWWGG 1 cut(s) 411
Eco47I GGWCC 2 cut(s) 50, 473
Eco57I CTGAAG 3 cut(s) 187, 411, 459
EcoO109I RGGNCCY 1 cut(s) 50
EcoT14I CCWWGG 1 cut(s) 411
ErhI CCWWGG 1 cut(s) 411
FalI AAGNNNNNCTT 2 cut(s) 97, 129
FaqI GGGAC 1 cut(s) 526
FauI CCCGC 1 cut(s) 524
FbaI TGATCA 1 cut(s) 495
Fnu4HI GCNGC 1 cut(s) 506
FokI GGATG 1 cut(s) 166
Fsp4HI GCNGC 1 cut(s) 506
FspBI CTAG 1 cut(s) 525
FspI TGCGCA 1 cut(s) 93
GlaI GCGC 3 cut(s) 93, 456, 458
GluI GCNGC 1 cut(s) 506
HapII CCGG 1 cut(s) 295
HhaI GCGC 3 cut(s) 94, 457, 459
Hin6I GCGC 3 cut(s) 92, 455, 457
HinP1I GCGC 3 cut(s) 92, 455, 457
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HinfI GANTC 1 cut(s) 416
HpaII CCGG 1 cut(s) 295
HphI GGTGA 2 cut(s) 91, 183
Hpy166II GTNNAC 2 cut(s) 427, 550
Hpy188I TCNGA 2 cut(s) 45, 206
Hpy188III TCNNGA 1 cut(s) 230
Hpy8I GTNNAC 2 cut(s) 427, 550
HpyAV CCTTC 1 cut(s) 173
HpyCH4III ACNGT 1 cut(s) 216
HpyCH4V TGCA 2 cut(s) 336, 505
HpyF10VI GCNNNNNNNGC 1 cut(s) 317
HpyF3I CTNAG 1 cut(s) 9
HspAI GCGC 3 cut(s) 92, 455, 457
Ksp22I TGATCA 1 cut(s) 495
Kzo9I GATC 4 cut(s) 81, 340, 495, 520
LmnI GCTCC 1 cut(s) 175
LpnPI CCDG 5 cut(s) 66, 287, 308, 371, 449
Lsp1109I GCAGC 1 cut(s) 517
LweI GCATC 1 cut(s) 307
MaeI CTAG 1 cut(s) 525
MaeIII GTNAC 2 cut(s) 216, 259
MalI GATC 4 cut(s) 83, 342, 497, 522
MboI GATC 4 cut(s) 81, 340, 495, 520
MboII GAAGA 3 cut(s) 176, 193, 283
MluCI AATT 4 cut(s) 127, 238, 491, 574
MnlI CCTC 6 cut(s) 39, 58, 237, 322, 486, 521
MseI TTAA 2 cut(s) 209, 489
MslI CAYNNNNRTG 1 cut(s) 306
MspI CCGG 1 cut(s) 295
MvnI CGCG 1 cut(s) 457
MwoI GCNNNNNNNGC 1 cut(s) 317
NdeII GATC 4 cut(s) 81, 340, 495, 520
NlaIV GGNNCC 1 cut(s) 51
NmuCI GTSAC 1 cut(s) 216
NsbI TGCGCA 1 cut(s) 93
PauI GCGCGC 1 cut(s) 455
PfeI GAWTC 1 cut(s) 416
PkrI GCNGC 1 cut(s) 507
PpuMI RGGWCCY 1 cut(s) 50
PshBI ATTAAT 1 cut(s) 209
Psp5II RGGWCCY 1 cut(s) 50
PspN4I GGNNCC 1 cut(s) 51
PspPI GGNCC 2 cut(s) 50, 473
PspPPI RGGWCCY 1 cut(s) 50
PstI CTGCAG 1 cut(s) 507
PteI GCGCGC 1 cut(s) 455
RsaI GTAC 2 cut(s) 98, 359
RsaNI GTAC 2 cut(s) 97, 358
RseI CAYNNNNRTG 1 cut(s) 306
SaqAI TTAA 2 cut(s) 209, 489
SatI GCNGC 1 cut(s) 506
Sau3AI GATC 4 cut(s) 81, 340, 495, 520
Sau96I GGNCC 2 cut(s) 50, 473
ScaI AGTACT 1 cut(s) 98
SetI ASST 5 cut(s) 15, 165, 172, 196, 558
SfaNI GCATC 1 cut(s) 307
SfcI CTRYAG 2 cut(s) 461, 503
SinI GGWCC 2 cut(s) 50, 473
SmiMI CAYNNNNRTG 1 cut(s) 306
SmlI CTYRAG 1 cut(s) 228
SmoI CTYRAG 1 cut(s) 228
Sse9I AATT 4 cut(s) 127, 238, 491, 574
SsiI CCGC 1 cut(s) 531
SspMI CTAG 1 cut(s) 525
StyI CCWWGG 1 cut(s) 411
TaaI ACNGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 304
TasI AATT 4 cut(s) 127, 238, 491, 574
TatI WGTACW 2 cut(s) 96, 357
TfiI GAWTC 1 cut(s) 416
Tru1I TTAA 2 cut(s) 209, 489
Tru9I TTAA 2 cut(s) 209, 489
TscAI CASTG 1 cut(s) 343
TseFI GTSAC 1 cut(s) 216
TseI GCWGC 1 cut(s) 505
Tsp45I GTSAC 1 cut(s) 216
TspDTI ATGAA 2 cut(s) 567, 586
TspRI CASTG 1 cut(s) 343
VpaK11BI GGWCC 2 cut(s) 50, 473
VspI ATTAAT 1 cut(s) 209
XapI RAATTY 2 cut(s) 127, 238
XspI CTAG 1 cut(s) 525
ZrmI AGTACT 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.