Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002033

Physical Location & Seq

Genomic Coordinates Forward (+)

1397343 .. 1399547

Gene Structure 2205 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1019300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1053 bp

ATGGCCATGACCAATGTCCAAGAAATGTCTATGAATGGTGAGCAGCCACCTGCTGAATATATTGTTAAAGAAAGTAGGTTTGGATCCATAGATTCTTCTCCACCATTGGCTGATGAAATCCCCATTATTGATATCAGTCTCTTCTCCCCATTATCTGCTCAATATTCTGAACAAGCAGAAAATCATGAGCTACAGAAACTCAGATCAGCACTCAGCTCGGCAGGATGCTTCCAGGCAATAGGCCATGGGATTTCGGATTCACTTCTTGACAAGGTACGTGAAGCAGCAAAACAATTCTTTGCACTTCCAGTAGAAGAGAAGGAAAAATACTCCCGCGCAGTTCATGGCGGAGCCGAAGGATATGGGAACGATGTCGTAGTATCGGAAAAGCAAGTTCTTGACTGGTCCTATCGTCTCACTCTCAGGGTCTTCCCAGAAAACCAAAGAAGGCTTCATCTTTGGCCTCAAAATCCAAATGATTTTGGAGAGATGTTACATGAATATGCAACGAAGGTAAAGTTGATGATGGGTATTGTGTTCAAGGCAATGGCAAAGTCGTTGAATTTGGAAGATAACAGCTTTGCAAAGCAGCTGTTTGGAGACCAATCCCTGATGCAAGCAAGGTTCAACTTCTATCCTCCGTGCTCGAGATCTGACCTCGTCCTCGGCGTCAAGCCTCACACGGATAGGTCCGGCGTGACATGTCTCTTGCAAGACAAGGATGTGGAAGGCCTTCAAGTTTTGATAGATGGAAAATGGGTTAGAGTCCCCATTGTTCCTCATGCTATCGTTCTTAATCTTGGCGATCAGATGCAGATCATGAGTAATGGTATTTACAAGAGCCCAATGCACAGGGTGGTGACAAATGGAGAGATTATGAGGCTATCAGTTGCCTTGTTCAATGAACCCGATCCTGAAACCCAGATTGGTCCCGTCGAGGACTTGATCGACGAGACAAGGCCGAGGTTGTACAAAAATGTCAAGAATTATGGTCGTATTAACTACGAATGCTATCAGAGAGGGGAAATTGCACTCGAAACAGTCAGATTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

351

Amino Acids

39.56

Weight (kDa)

5.48

Isoelectric Point (pI)

40.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	40 - 157	1e-25	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	208 - 303	3.2e-31	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002033)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g02111 FvH4_1g02112 FvH4_1g02120
malus_domestica	MD02G1019300.v1.1 MD02G1019600.v1.1
prunus_persica	Prupe.7G251800_v2.0.a1 Prupe.7G251800_v2.0.a1
pyrus_communis	pycom02g01720 pycom02g01730 pycom02g01740
rosa_chinensis	RchiOBHm_Chr2g0087171 RchiOBHm_Chr2g0087181 RchiOBHm_Chr2g0087191 RchiOBHm_Chr2g0087251 RchiOBHm_Chr6g0295511
rosa_laevigata	RLG00000011795 RLG00000015839 RLG00000015840 RLG00000015841 RLG00000015843 RLG00000015847
rosa_multiflora	Rmu_co8200202.1_g000001 Rmu_sc0003367.1_g000007 Rmu_sc0009355.1_g000005 Rmu_sc0009802.1_g000001 Rmu_sc0011562.1_g000001 Rmu_sc0018794.1_g000006 Rmu_ssc0000239.1_g000011
rosa_roxburghii	Rroxscaffold_2G00153820 Rroxscaffold_7G00172410
rosa_rugosa	Rorug01G0473200 Rorug01G0473600 Rorug06G0251600
rosa_samantha	Rh2AG026300 Rh2AG026500 Rh2AG027000 Rh2CG026800 Rh2CG026900 Rh2CG027000 Rh2CG027400 Rh2DG026600 Rh6BG373100 Rh6CG379300 Rh6DG365800
rosa_wichuraiana	Rw2G002110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	58
Acc36I	ACCTGC	1 cut(s)	58
AccII	CGCG	1 cut(s)	336
AciI	CCGC	2 cut(s)	334, 348
AclWI	GGATC	3 cut(s)	78, 91, 905
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	1 cut(s)	562
AcyI	GRCGYC	1 cut(s)	669
AdeI	CACNNNGTG	1 cut(s)	856
AfaI	GTAC	2 cut(s)	276, 971
AflIII	ACRYGT	1 cut(s)	701
AgsI	TTSAA	5 cut(s)	541, 562, 628, 737, 901
AjnI	CCWGG	1 cut(s)	231
AjuI	GAANNNNNNNTTGG	6 cut(s)	63, 95, 435, 467, 909, 941
AluBI	AGCT	4 cut(s)	190, 216, 579, 592
AluI	AGCT	4 cut(s)	190, 216, 579, 592
Alw21I	GWGCWC	1 cut(s)	647
Alw26I	GTCTC	5 cut(s)	143, 419, 594, 710, 947
AlwI	GGATC	3 cut(s)	78, 91, 905
Ama87I	CYCGRG	1 cut(s)	646
AoxI	GGCC	5 cut(s)	3, 241, 461, 730, 959
ApeKI	GCWGC	3 cut(s)	43, 284, 589
ApoI	RAATTY	1 cut(s)	562
Asp700I	GAANNNNTTC	1 cut(s)	732
AspLEI	GCGC	1 cut(s)	338
AspS9I	GGNCC	3 cut(s)	405, 690, 929
AsuHPI	GGTGA	2 cut(s)	50, 871
AvaI	CYCGRG	1 cut(s)	646
AvaII	GGWCC	3 cut(s)	405, 690, 929
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	83
BanII	GRGCYC	1 cut(s)	845
BarI	GAAGNNNNNNTAC	2 cut(s)	311, 343
BbsI	GAAGAC	1 cut(s)	421
Bbv12I	GWGCWC	1 cut(s)	647
BbvI	GCAGC	3 cut(s)	55, 296, 601
BccI	CCATC	2 cut(s)	520, 743
BcgI	CGANNNNNNTGC	2 cut(s)	198, 232
BciT130I	CCWGG	1 cut(s)	233
BcoDI	GTCTC	5 cut(s)	143, 419, 594, 710, 947
BfmI	CTRYAG	1 cut(s)	191
BfuAI	ACCTGC	1 cut(s)	58
BglII	AGATCT	1 cut(s)	650
BisI	GCNGC	3 cut(s)	44, 285, 590
BlsI	GCNGC	3 cut(s)	45, 286, 591
Bme1390I	CCNGG	1 cut(s)	233
Bme18I	GGWCC	3 cut(s)	405, 690, 929
BmeT110I	CYCGRG	1 cut(s)	646
BmgT120I	GGNCC	3 cut(s)	405, 690, 929
BmiI	GGNNCC	3 cut(s)	85, 352, 931
BmrFI	CCNGG	1 cut(s)	233
BmsI	GCATC	3 cut(s)	215, 603, 801
BoxI	GACNNNNGTC	1 cut(s)	14
BpiI	GAAGAC	1 cut(s)	421
BsaAI	YACGTR	1 cut(s)	278
BsaBI	GATNNNNATC	1 cut(s)	815
BsaHI	GRCGYC	1 cut(s)	669
BsaI	GGTCTC	1 cut(s)	594
BsaJI	CCNNGG	3 cut(s)	244, 664, 962
Bse1I	ACTGG	2 cut(s)	308, 407
Bse3DI	GCAATG	1 cut(s)	552
Bse8I	GATNNNNATC	1 cut(s)	815
BseBI	CCWGG	1 cut(s)	233
BseDI	CCNNGG	3 cut(s)	244, 664, 962
BseGI	GGATG	2 cut(s)	230, 727
BseJI	GATNNNNATC	1 cut(s)	815
BseMI	GCAATG	1 cut(s)	552
BseMII	CTCAG	3 cut(s)	214, 226, 436
BseNI	ACTGG	2 cut(s)	308, 407
BseXI	GCAGC	3 cut(s)	55, 296, 601
Bsh1236I	CGCG	1 cut(s)	336
BshFI	GGCC	5 cut(s)	5, 243, 463, 732, 961
BsiHKAI	GWGCWC	1 cut(s)	647
BsiHKCI	CYCGRG	1 cut(s)	646
BsiSI	CCGG	1 cut(s)	693
BslFI	GGGAC	2 cut(s)	752, 915
BsmAI	GTCTC	5 cut(s)	143, 419, 594, 710, 947
BsmBI	CGTCTC	1 cut(s)	419
BsmFI	GGGAC	2 cut(s)	752, 915
BsmI	GAATGC	1 cut(s)	1013
BsnI	GGCC	5 cut(s)	5, 243, 463, 732, 961
Bso31I	GGTCTC	1 cut(s)	594
BsoBI	CYCGRG	1 cut(s)	646
Bsp1286I	GDGCHC	2 cut(s)	647, 845
Bsp1407I	TGTACA	1 cut(s)	969
Bsp143I	GATC	7 cut(s)	83, 203, 650, 805, 816, 910, 945
Bsp19I	CCATGG	1 cut(s)	244
BspACI	CCGC	2 cut(s)	334, 348
BspANI	GGCC	5 cut(s)	5, 243, 463, 732, 961
BspCNI	CTCAG	3 cut(s)	213, 225, 435
BspFNI	CGCG	1 cut(s)	336
BspHI	TCATGA	2 cut(s)	184, 819
BspLI	GGNNCC	3 cut(s)	85, 352, 931
BspMI	ACCTGC	1 cut(s)	58
BspPI	GGATC	3 cut(s)	78, 91, 905
BspTNI	GGTCTC	1 cut(s)	594
BsrDI	GCAATG	1 cut(s)	552
BsrGI	TGTACA	1 cut(s)	969
BsrI	ACTGG	2 cut(s)	308, 407
BssECI	CCNNGG	3 cut(s)	244, 664, 962
BssMI	GATC	7 cut(s)	83, 203, 650, 805, 816, 910, 945
BssNI	GRCGYC	1 cut(s)	669
BssT1I	CCWWGG	1 cut(s)	244
Bst2UI	CCWGG	1 cut(s)	233
Bst4CI	ACNGT	1 cut(s)	1042
Bst6I	CTCTTC	2 cut(s)	146, 309
BstACI	GRCGYC	1 cut(s)	669
BstAUI	TGTACA	1 cut(s)	969
BstBAI	YACGTR	1 cut(s)	278
BstC8I	GCNNGC	1 cut(s)	618
BstDEI	CTNAG	3 cut(s)	200, 212, 422
BstDSI	CCRYGG	1 cut(s)	244
BstF5I	GGATG	2 cut(s)	230, 727
BstFNI	CGCG	1 cut(s)	336
BstHHI	GCGC	1 cut(s)	338
BstKTI	GATC	7 cut(s)	86, 206, 653, 808, 819, 913, 948
BstMAI	GTCTC	5 cut(s)	143, 419, 594, 710, 947
BstMBI	GATC	7 cut(s)	83, 203, 650, 805, 816, 910, 945
BstNI	CCWGG	1 cut(s)	233
BstNSI	RCATGY	1 cut(s)	705
BstPAI	GACNNNNGTC	1 cut(s)	14
BstSCI	CCNGG	1 cut(s)	231
BstSFI	CTRYAG	1 cut(s)	191
BstUI	CGCG	1 cut(s)	336
BstV1I	GCAGC	3 cut(s)	55, 296, 601
BstV2I	GAAGAC	1 cut(s)	421
BstX2I	RGATCY	2 cut(s)	83, 650
BstYI	RGATCY	2 cut(s)	83, 650
BsuRI	GGCC	5 cut(s)	5, 243, 463, 732, 961
BtgI	CCRYGG	1 cut(s)	244
BtsCI	GGATG	2 cut(s)	230, 727
BveI	ACCTGC	1 cut(s)	58
Cac8I	GCNNGC	1 cut(s)	618
CciI	TCATGA	2 cut(s)	184, 819
CfoI	GCGC	1 cut(s)	338
Cfr13I	GGNCC	3 cut(s)	405, 690, 929
CseI	GACGC	1 cut(s)	658
Csp6I	GTAC	2 cut(s)	275, 970
CviAII	CATG	8 cut(s)	7, 185, 245, 344, 497, 702, 782, 820
CviQI	GTAC	2 cut(s)	275, 970
DdeI	CTNAG	3 cut(s)	200, 212, 422
DpnI	GATC	7 cut(s)	85, 205, 652, 807, 818, 912, 947
DpnII	GATC	7 cut(s)	83, 203, 650, 805, 816, 910, 945
DraIII	CACNNNGTG	1 cut(s)	856
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	2 cut(s)	146, 309
EarI	CTCTTC	2 cut(s)	146, 309
EciI	GGCGGA	1 cut(s)	363
Eco130I	CCWWGG	1 cut(s)	244
Eco147I	AGGCCT	1 cut(s)	732
Eco24I	GRGCYC	1 cut(s)	845
Eco31I	GGTCTC	1 cut(s)	594
Eco32I	GATATC	1 cut(s)	133
Eco47I	GGWCC	3 cut(s)	405, 690, 929
Eco88I	CYCGRG	1 cut(s)	646
EcoRII	CCWGG	1 cut(s)	231
EcoRV	GATATC	1 cut(s)	133
EcoT14I	CCWWGG	1 cut(s)	244
EcoT38I	GRGCYC	1 cut(s)	845
ErhI	CCWWGG	1 cut(s)	244
Esp3I	CGTCTC	1 cut(s)	419
FaeI	CATG	8 cut(s)	10, 188, 248, 347, 500, 705, 785, 823
FaqI	GGGAC	2 cut(s)	752, 915
FatI	CATG	8 cut(s)	6, 184, 244, 343, 496, 701, 781, 819
FauI	CCCGC	1 cut(s)	341
Fnu4HI	GCNGC	3 cut(s)	44, 285, 590
FokI	GGATG	2 cut(s)	237, 734
FriOI	GRGCYC	1 cut(s)	845
Fsp4HI	GCNGC	3 cut(s)	44, 285, 590
GlaI	GCGC	1 cut(s)	337
GluI	GCNGC	3 cut(s)	44, 285, 590
HaeIII	GGCC	5 cut(s)	5, 243, 463, 732, 961
HapII	CCGG	1 cut(s)	693
HgaI	GACGC	1 cut(s)	658
HhaI	GCGC	1 cut(s)	338
Hin1I	GRCGYC	1 cut(s)	669
Hin1II	CATG	8 cut(s)	10, 188, 248, 347, 500, 705, 785, 823
Hin6I	GCGC	1 cut(s)	336
HinP1I	GCGC	1 cut(s)	336
HinfI	GANTC	4 cut(s)	92, 257, 765, 1047
HpaII	CCGG	1 cut(s)	693
HphI	GGTGA	2 cut(s)	50, 871
Hpy188I	TCNGA	8 cut(s)	169, 203, 256, 385, 655, 810, 1017, 1046
Hpy188III	TCNNGA	7 cut(s)	185, 266, 398, 648, 820, 914, 982
Hpy99I	CGWCG	2 cut(s)	938, 953
HpyAV	CCTTC	6 cut(s)	313, 350, 441, 505, 722, 743
HpyCH4III	ACNGT	1 cut(s)	1042
HpyCH4IV	ACGT	1 cut(s)	277
HpyCH4V	TGCA	8 cut(s)	302, 506, 584, 616, 712, 814, 850, 1031
HpyF3I	CTNAG	3 cut(s)	200, 212, 422
HpySE526I	ACGT	1 cut(s)	277
Hsp92I	GRCGYC	1 cut(s)	669
Hsp92II	CATG	8 cut(s)	10, 188, 248, 347, 500, 705, 785, 823
HspAI	GCGC	1 cut(s)	336
Kzo9I	GATC	7 cut(s)	83, 203, 650, 805, 816, 910, 945
LmnI	GCTCC	1 cut(s)	350
Lsp1109I	GCAGC	3 cut(s)	55, 296, 601
LweI	GCATC	3 cut(s)	215, 603, 801
MaeII	ACGT	1 cut(s)	277
MaeIII	GTNAC	3 cut(s)	492, 697, 859
MalI	GATC	7 cut(s)	85, 205, 652, 807, 818, 912, 947
MboI	GATC	7 cut(s)	83, 203, 650, 805, 816, 910, 945
MboII	GAAGA	5 cut(s)	87, 133, 326, 421, 581
MflI	RGATCY	2 cut(s)	83, 650
MhlI	GDGCHC	2 cut(s)	647, 845
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	4 cut(s)	293, 562, 985, 1026
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	1 cut(s)	774
Mox20I	TGGCCA	1 cut(s)	5
MroXI	GAANNNNTTC	1 cut(s)	732
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	3 cut(s)	66, 795, 999
MslI	CAYNNNNRTG	1 cut(s)	501
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	592
MspI	CCGG	1 cut(s)	693
MspR9I	CCNGG	1 cut(s)	233
Mva1269I	GAATGC	1 cut(s)	1013
MvaI	CCWGG	1 cut(s)	233
MvnI	CGCG	1 cut(s)	336
NcoI	CCATGG	1 cut(s)	244
NdeII	GATC	7 cut(s)	83, 203, 650, 805, 816, 910, 945
NlaIII	CATG	8 cut(s)	10, 188, 248, 347, 500, 705, 785, 823
NlaIV	GGNNCC	3 cut(s)	85, 352, 931
NmeAIII	GCCGAG	3 cut(s)	197, 645, 987
NmuCI	GTSAC	2 cut(s)	697, 859
NspI	RCATGY	1 cut(s)	705
PaeR7I	CTCGAG	1 cut(s)	646
PagI	TCATGA	2 cut(s)	184, 819
PaqCI	CACCTGC	1 cut(s)	58
PceI	AGGCCT	1 cut(s)	732
PciI	ACATGT	1 cut(s)	701
PcsI	WCGNNNNNNNCGW	1 cut(s)	666
PctI	GAATGC	1 cut(s)	1013
PdmI	GAANNNNTTC	1 cut(s)	732
PfeI	GAWTC	3 cut(s)	92, 257, 1047
PflFI	GACNNNGTC	1 cut(s)	659
PkrI	GCNGC	3 cut(s)	45, 286, 591
PleI	GAGTC	1 cut(s)	773
PpsI	GAGTC	1 cut(s)	773
Ppu21I	YACGTR	1 cut(s)	278
PscI	ACATGT	1 cut(s)	701
PshAI	GACNNNNGTC	1 cut(s)	14
Psp6I	CCWGG	1 cut(s)	231
PspGI	CCWGG	1 cut(s)	231
PspN4I	GGNNCC	3 cut(s)	85, 352, 931
PspPI	GGNCC	3 cut(s)	405, 690, 929
PsuI	RGATCY	2 cut(s)	83, 650
PsyI	GACNNNGTC	1 cut(s)	659
PvuII	CAGCTG	1 cut(s)	592
RsaI	GTAC	2 cut(s)	276, 971
RsaNI	GTAC	2 cut(s)	275, 970
RseI	CAYNNNNRTG	1 cut(s)	501
SaqAI	TTAA	3 cut(s)	66, 795, 999
SatI	GCNGC	3 cut(s)	44, 285, 590
Sau3AI	GATC	7 cut(s)	83, 203, 650, 805, 816, 910, 945
Sau96I	GGNCC	3 cut(s)	405, 690, 929
SchI	GAGTC	1 cut(s)	774
ScrFI	CCNGG	1 cut(s)	233
SduI	GDGCHC	2 cut(s)	647, 845
SfaNI	GCATC	3 cut(s)	215, 603, 801
SfcI	CTRYAG	1 cut(s)	191
Sfr274I	CTCGAG	1 cut(s)	646
SinI	GGWCC	3 cut(s)	405, 690, 929
SlaI	CTCGAG	1 cut(s)	646
SmiMI	CAYNNNNRTG	1 cut(s)	501
SmlI	CTYRAG	1 cut(s)	646
SmoI	CTYRAG	1 cut(s)	646
Sse9I	AATT	4 cut(s)	293, 562, 985, 1026
SseBI	AGGCCT	1 cut(s)	732
SsiI	CCGC	2 cut(s)	334, 348
SspI	AATATT	1 cut(s)	164
StuI	AGGCCT	1 cut(s)	732
StyD4I	CCNGG	1 cut(s)	231
StyI	CCWWGG	1 cut(s)	244
TaaI	ACNGT	1 cut(s)	1042
TaiI	ACGT	1 cut(s)	280
TaqI	TCGA	4 cut(s)	647, 936, 948, 1035
TasI	AATT	4 cut(s)	293, 562, 985, 1026
TatI	WGTACW	1 cut(s)	969
TfiI	GAWTC	3 cut(s)	92, 257, 1047
Tru1I	TTAA	3 cut(s)	66, 795, 999
Tru9I	TTAA	3 cut(s)	66, 795, 999
TseFI	GTSAC	2 cut(s)	697, 859
TseI	GCWGC	3 cut(s)	43, 284, 589
Tsp45I	GTSAC	2 cut(s)	697, 859
TspDTI	ATGAA	6 cut(s)	47, 129, 332, 443, 513, 918
TspGWI	ACGGA	2 cut(s)	630, 698
Tth111I	GACNNNGTC	1 cut(s)	659
VpaK11BI	GGWCC	3 cut(s)	405, 690, 929
XapI	RAATTY	1 cut(s)	562
XceI	RCATGY	1 cut(s)	705
XhoI	CTCGAG	1 cut(s)	646
XmnI	GAANNNNTTC	1 cut(s)	732

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.