RchiOBHm_Chr2g0087251

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002033
Physical Location & Seq
Forward (+)
2080041 .. 2080553
513 bp
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UTR
Exon/CDS
Intron
PRQ46270

Sequence Viewer

Length: 408 bp
ATGATGGGTGTTCTGTTTAAGACCATGGCGAAATCGTTGAATTCGGAAGAGAACGACTTTTTTACTAAGCTGCTTGGAGAGGTAGCTCTGATGCAAGCAAGATTCAACTTTTATTCCGCTGTGTTCAAAAGCCGATCAGGTCTTGGTGTGTCGGCACACATAGACAGGTCAGGAATAAGAGTTCTGTTGCAGGACAAAGAAGTAGAGGGTCTTCAAGTTTTGGTGGATGGTAAATGGGATACAGTCCCCATTGTGCCACAGGCCATTGTTGTTAATCTTAGTGATCAAGTGCAGATTATGAATAATGGCATATTCAAGGGCCCACTGCACAGGGTGATGAAGCTATCTGTGGCCTTGCTTAATGAGAACCAGATCCTGATACTGAGATTAGTCCAGTGGAGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

135

Amino Acids

15.2

Weight (kDa)

9.45

Isoelectric Point (pI)

25.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 34 - 113 9.3e-19 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 117
AclWI GGATC 1 cut(s) 367
AcsI RAATTY 1 cut(s) 40
AdeI CACNNNGTG 1 cut(s) 334
AgsI TTSAA 5 cut(s) 40, 106, 127, 215, 316
AloI GAACNNNNNNTCC 2 cut(s) 165, 197
AluBI AGCT 3 cut(s) 70, 86, 343
AluI AGCT 3 cut(s) 70, 86, 343
AlwI GGATC 1 cut(s) 367
AlwNI CAGNNNCTG 1 cut(s) 376
AoxI GGCC 3 cut(s) 261, 319, 351
ApaI GGGCCC 1 cut(s) 323
ApeKI GCWGC 1 cut(s) 70
ApoI RAATTY 1 cut(s) 40
AspS9I GGNCC 2 cut(s) 319, 320
AsuHPI GGTGA 1 cut(s) 346
BaeGI GKGCMC 1 cut(s) 323
BanII GRGCYC 1 cut(s) 323
BarI GAAGNNNNNNTAC 2 cut(s) 195, 227
BbsI GAAGAC 1 cut(s) 203
BbvI GCAGC 1 cut(s) 57
BccI CCATC 1 cut(s) 221
BciVI GTATCC 1 cut(s) 232
BclI TGATCA 1 cut(s) 283
BfuI GTATCC 1 cut(s) 232
BisI GCNGC 1 cut(s) 71
BlsI GCNGC 1 cut(s) 72
BmgT120I GGNCC 2 cut(s) 319, 320
BmiI GGNNCC 1 cut(s) 321
BmsI GCATC 1 cut(s) 81
BpiI GAAGAC 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 24
Bse1I ACTGG 1 cut(s) 394
BseDI CCNNGG 1 cut(s) 24
BseGI GGATG 1 cut(s) 232
BseMII CTCAG 1 cut(s) 374
BseNI ACTGG 1 cut(s) 394
BseSI GKGCMC 1 cut(s) 323
BseXI GCAGC 1 cut(s) 57
BsgI GTGCAG 2 cut(s) 311, 311
BshFI GGCC 3 cut(s) 263, 321, 353
BslFI GGGAC 1 cut(s) 230
BsmFI GGGAC 1 cut(s) 230
BsnI GGCC 3 cut(s) 263, 321, 353
Bsp120I GGGCCC 1 cut(s) 319
Bsp1286I GDGCHC 1 cut(s) 323
Bsp143I GATC 3 cut(s) 134, 283, 372
Bsp19I CCATGG 1 cut(s) 24
BspACI CCGC 1 cut(s) 117
BspANI GGCC 3 cut(s) 263, 321, 353
BspCNI CTCAG 1 cut(s) 375
BspLI GGNNCC 1 cut(s) 321
BspPI GGATC 1 cut(s) 367
BsrI ACTGG 1 cut(s) 394
BssECI CCNNGG 1 cut(s) 24
BssMI GATC 3 cut(s) 134, 283, 372
BssT1I CCWWGG 1 cut(s) 24
Bst4CI ACNGT 1 cut(s) 244
Bst6I CTCTTC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 3 cut(s) 66, 278, 383
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 1 cut(s) 232
BstKTI GATC 3 cut(s) 137, 286, 375
BstMBI GATC 3 cut(s) 134, 283, 372
BstSLI GKGCMC 1 cut(s) 323
BstV1I GCAGC 1 cut(s) 57
BstV2I GAAGAC 1 cut(s) 203
BstX2I RGATCY 1 cut(s) 372
BstYI RGATCY 1 cut(s) 372
BsuI GTATCC 1 cut(s) 232
BsuRI GGCC 3 cut(s) 263, 321, 353
BtgI CCRYGG 1 cut(s) 24
BtsCI GGATG 1 cut(s) 232
BtsI GCAGTG 1 cut(s) 323
BtsIMutI CAGTG 2 cut(s) 323, 401
Cac8I GCNNGC 1 cut(s) 96
CaiI CAGNNNCTG 1 cut(s) 376
Cfr13I GGNCC 2 cut(s) 319, 320
CviAII CATG 1 cut(s) 25
CviJI RGCY 7 cut(s) 70, 86, 132, 263, 321, 343, 353
CviKI_1 RGCY 7 cut(s) 70, 86, 132, 263, 321, 343, 353
DdeI CTNAG 3 cut(s) 66, 278, 383
DpnI GATC 3 cut(s) 136, 285, 374
DpnII GATC 3 cut(s) 134, 283, 372
DraIII CACNNNGTG 1 cut(s) 334
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 24
Eco24I GRGCYC 1 cut(s) 323
EcoO109I RGGNCCY 1 cut(s) 319
EcoRI GAATTC 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 24
EcoT38I GRGCYC 1 cut(s) 323
ErhI CCWWGG 1 cut(s) 24
FaeI CATG 1 cut(s) 28
FaiI YATR 4 cut(s) 26, 161, 299, 311
FaqI GGGAC 1 cut(s) 230
FatI CATG 1 cut(s) 24
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 1 cut(s) 71
FokI GGATG 1 cut(s) 239
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 1 cut(s) 71
GluI GCNGC 1 cut(s) 71
HaeIII GGCC 3 cut(s) 263, 321, 353
Hin1II CATG 1 cut(s) 28
HinfI GANTC 1 cut(s) 102
HphI GGTGA 1 cut(s) 346
Hpy188I TCNGA 2 cut(s) 46, 90
Hpy188III TCNNGA 2 cut(s) 171, 376
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4V TGCA 4 cut(s) 94, 190, 292, 328
HpyF3I CTNAG 3 cut(s) 66, 278, 383
Hsp92II CATG 1 cut(s) 28
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 3 cut(s) 134, 283, 372
LpnPI CCDG 8 cut(s) 123, 151, 156, 176, 245, 316, 383, 389
Lsp1109I GCAGC 1 cut(s) 57
LweI GCATC 1 cut(s) 81
MalI GATC 3 cut(s) 136, 285, 374
MboI GATC 3 cut(s) 134, 283, 372
MboII GAAGA 2 cut(s) 59, 203
MflI RGATCY 1 cut(s) 372
MhlI GDGCHC 1 cut(s) 323
MluCI AATT 1 cut(s) 40
MnlI CCTC 2 cut(s) 73, 199
MseI TTAA 3 cut(s) 18, 273, 360
MspA1I CMGCKG 1 cut(s) 119
NcoI CCATGG 1 cut(s) 24
NdeII GATC 3 cut(s) 134, 283, 372
NlaIII CATG 1 cut(s) 28
NlaIV GGNNCC 1 cut(s) 321
PfeI GAWTC 1 cut(s) 102
PkrI GCNGC 1 cut(s) 72
PspN4I GGNNCC 1 cut(s) 321
PspOMI GGGCCC 1 cut(s) 319
PspPI GGNCC 2 cut(s) 319, 320
PstNI CAGNNNCTG 1 cut(s) 376
PsuI RGATCY 1 cut(s) 372
SaqAI TTAA 3 cut(s) 18, 273, 360
SatI GCNGC 1 cut(s) 71
Sau3AI GATC 3 cut(s) 134, 283, 372
Sau96I GGNCC 2 cut(s) 319, 320
SduI GDGCHC 1 cut(s) 323
SetI ASST 6 cut(s) 72, 84, 88, 142, 170, 345
SfaNI GCATC 1 cut(s) 81
Sse9I AATT 1 cut(s) 40
SsiI CCGC 1 cut(s) 117
StyI CCWWGG 1 cut(s) 24
TaaI ACNGT 1 cut(s) 244
TasI AATT 1 cut(s) 40
TfiI GAWTC 1 cut(s) 102
Tru1I TTAA 3 cut(s) 18, 273, 360
Tru9I TTAA 3 cut(s) 18, 273, 360
TscAI CASTG 2 cut(s) 330, 401
TseI GCWGC 1 cut(s) 70
TspDTI ATGAA 2 cut(s) 314, 353
TspRI CASTG 2 cut(s) 330, 401
XapI RAATTY 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.