pycom02g01720

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002033
Physical Location & Seq
Forward (+)
1025806 .. 1026932
1127 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g01720.1

Sequence Viewer

Length: 519 bp
ATGGCGGCGCCGAAGGATAATCGCAACGATGTCGTAGTATCGGAAAAGCAAGTTCTTGACTGGTCCTGTCGTCTCACTCTCAGGCAAGGTTCAACTTCTATCCTCCGTGCTCGAGATCTGACCTCTGTCCTCGGCGTCAAGCCTCACACGGATAGGTCCGGTGTGACATGTCTCTTGCAAGACAAGGATGCGGAAGGCCTTCAAGTATTGATAGATGAAAAATGGGTTAGAGTCCCCATTCTTCCTCATGCTATCGTTCTTAATCTCGGTGATCAGATGCAGATCATGAGTAATGGGATTTACAAGAGCGCCATGCACAGAGTGGTGACAAATGCAGAGAGGATGAGGTTATCTGTTGTTTTGTTCAATGAACCCGATCCTGAAACCGAGATTGGTCCGGTCGAGGACTTGATCGACGAGACAAGGCCGAGGTTGTACAAGAGTGTCACGAATTATGCTTCTATCAACTCCGAAAGCTATTATAGAGGGGAAATTGCACTCGAAACAGTCAGAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

173

Amino Acids

19.4

Weight (kDa)

5.65

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 42 - 125 1.4e-25 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 7
AciI CCGC 2 cut(s) 5, 191
AclWI GGATC 1 cut(s) 371
AcyI GRCGYC 2 cut(s) 8, 135
AdeI CACNNNGTG 1 cut(s) 322
AfaI GTAC 1 cut(s) 437
AflIII ACRYGT 1 cut(s) 167
AgsI TTSAA 3 cut(s) 93, 203, 367
AjuI GAANNNNNNNTTGG 2 cut(s) 375, 407
AluBI AGCT 1 cut(s) 477
AluI AGCT 1 cut(s) 477
Alw21I GWGCWC 1 cut(s) 112
Alw26I GTCTC 3 cut(s) 77, 176, 413
AlwI GGATC 1 cut(s) 371
Ama87I CYCGRG 1 cut(s) 111
AoxI GGCC 2 cut(s) 196, 425
Asp700I GAANNNNTTC 1 cut(s) 198
AspLEI GCGC 2 cut(s) 10, 311
AspS9I GGNCC 3 cut(s) 63, 156, 395
AsuHPI GGTGA 2 cut(s) 281, 337
AvaI CYCGRG 1 cut(s) 111
AvaII GGWCC 3 cut(s) 63, 156, 395
BanI GGYRCC 1 cut(s) 7
Bbv12I GWGCWC 1 cut(s) 112
BcgI CGANNNNNNTGC 2 cut(s) 13, 47
BclI TGATCA 1 cut(s) 271
BcoDI GTCTC 3 cut(s) 77, 176, 413
BfoI RGCGCY 2 cut(s) 11, 312
BglII AGATCT 1 cut(s) 115
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme18I GGWCC 3 cut(s) 63, 156, 395
BmeT110I CYCGRG 1 cut(s) 111
BmgT120I GGNCC 3 cut(s) 63, 156, 395
BmiI GGNNCC 1 cut(s) 9
BmsI GCATC 2 cut(s) 178, 267
BoxI GACNNNNGTC 1 cut(s) 125
BsaBI GATNNNNATC 1 cut(s) 281
BsaHI GRCGYC 2 cut(s) 8, 135
BsaJI CCNNGG 2 cut(s) 130, 428
BsaWI WCCGGW 2 cut(s) 158, 397
Bse1I ACTGG 1 cut(s) 65
Bse8I GATNNNNATC 1 cut(s) 281
BseDI CCNNGG 2 cut(s) 130, 428
BseGI GGATG 2 cut(s) 193, 348
BseJI GATNNNNATC 1 cut(s) 281
BseMII CTCAG 1 cut(s) 94
BseNI ACTGG 1 cut(s) 65
Bsh1285I CGRYCG 1 cut(s) 402
BshFI GGCC 2 cut(s) 198, 427
BshNI GGYRCC 1 cut(s) 7
BsiEI CGRYCG 1 cut(s) 402
BsiHKAI GWGCWC 1 cut(s) 112
BsiHKCI CYCGRG 1 cut(s) 111
BsiSI CCGG 2 cut(s) 159, 398
BslFI GGGAC 1 cut(s) 218
BsmAI GTCTC 3 cut(s) 77, 176, 413
BsmBI CGTCTC 1 cut(s) 77
BsmFI GGGAC 1 cut(s) 218
BsnI GGCC 2 cut(s) 198, 427
BsoBI CYCGRG 1 cut(s) 111
Bsp1286I GDGCHC 1 cut(s) 112
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 5 cut(s) 115, 271, 282, 376, 411
BspACI CCGC 2 cut(s) 5, 191
BspANI GGCC 2 cut(s) 198, 427
BspCNI CTCAG 1 cut(s) 93
BspHI TCATGA 1 cut(s) 285
BspLI GGNNCC 1 cut(s) 9
BspPI GGATC 1 cut(s) 371
BspT107I GGYRCC 1 cut(s) 7
BsrGI TGTACA 1 cut(s) 435
BsrI ACTGG 1 cut(s) 65
BssECI CCNNGG 2 cut(s) 130, 428
BssMI GATC 5 cut(s) 115, 271, 282, 376, 411
BssNI GRCGYC 2 cut(s) 8, 135
Bst4CI ACNGT 1 cut(s) 508
BstACI GRCGYC 2 cut(s) 8, 135
BstAUI TGTACA 1 cut(s) 435
BstDEI CTNAG 1 cut(s) 80
BstF5I GGATG 2 cut(s) 193, 348
BstH2I RGCGCY 2 cut(s) 11, 312
BstHHI GCGC 2 cut(s) 10, 311
BstKTI GATC 5 cut(s) 118, 274, 285, 379, 414
BstMAI GTCTC 3 cut(s) 77, 176, 413
BstMBI GATC 5 cut(s) 115, 271, 282, 376, 411
BstMCI CGRYCG 1 cut(s) 402
BstNSI RCATGY 1 cut(s) 171
BstPAI GACNNNNGTC 1 cut(s) 125
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
BsuRI GGCC 2 cut(s) 198, 427
BtsCI GGATG 2 cut(s) 193, 348
CciI TCATGA 1 cut(s) 285
CfoI GCGC 2 cut(s) 10, 311
Cfr13I GGNCC 3 cut(s) 63, 156, 395
CseI GACGC 1 cut(s) 124
Csp6I GTAC 1 cut(s) 436
CviAII CATG 4 cut(s) 168, 248, 286, 313
CviJI RGCY 4 cut(s) 142, 198, 427, 477
CviKI_1 RGCY 4 cut(s) 142, 198, 427, 477
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 1 cut(s) 80
DinI GGCGCC 1 cut(s) 9
DpnI GATC 5 cut(s) 117, 273, 284, 378, 413
DpnII GATC 5 cut(s) 115, 271, 282, 376, 411
DraIII CACNNNGTG 1 cut(s) 322
Eco147I AGGCCT 1 cut(s) 198
Eco47I GGWCC 3 cut(s) 63, 156, 395
Eco88I CYCGRG 1 cut(s) 111
EgeI GGCGCC 1 cut(s) 9
EheI GGCGCC 1 cut(s) 9
Esp3I CGTCTC 1 cut(s) 77
FaeI CATG 4 cut(s) 171, 251, 289, 316
FaiI YATR 6 cut(s) 169, 249, 287, 314, 456, 483
FaqI GGGAC 1 cut(s) 218
FatI CATG 4 cut(s) 167, 247, 285, 312
FbaI TGATCA 1 cut(s) 271
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 2 cut(s) 200, 355
Fsp4HI GCNGC 1 cut(s) 6
GlaI GCGC 2 cut(s) 9, 310
GluI GCNGC 1 cut(s) 6
HaeII RGCGCY 2 cut(s) 11, 312
HaeIII GGCC 2 cut(s) 198, 427
HapII CCGG 2 cut(s) 159, 398
HgaI GACGC 1 cut(s) 124
HhaI GCGC 2 cut(s) 10, 311
Hin1I GRCGYC 2 cut(s) 8, 135
Hin1II CATG 4 cut(s) 171, 251, 289, 316
Hin6I GCGC 2 cut(s) 8, 309
HinP1I GCGC 2 cut(s) 8, 309
HinfI GANTC 2 cut(s) 231, 513
HpaII CCGG 2 cut(s) 159, 398
HphI GGTGA 2 cut(s) 281, 337
Hpy188I TCNGA 5 cut(s) 43, 120, 276, 472, 512
Hpy188III TCNNGA 5 cut(s) 56, 113, 286, 380, 448
Hpy99I CGWCG 1 cut(s) 419
HpyAV CCTTC 3 cut(s) 7, 188, 209
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4V TGCA 5 cut(s) 178, 280, 316, 335, 497
HpyF3I CTNAG 1 cut(s) 80
Hsp92I GRCGYC 2 cut(s) 8, 135
Hsp92II CATG 4 cut(s) 171, 251, 289, 316
HspAI GCGC 2 cut(s) 8, 309
KasI GGCGCC 1 cut(s) 7
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 5 cut(s) 115, 271, 282, 376, 411
LpnPI CCDG 6 cut(s) 46, 67, 79, 172, 393, 411
LweI GCATC 2 cut(s) 178, 267
MaeIII GTNAC 3 cut(s) 163, 325, 445
MalI GATC 5 cut(s) 117, 273, 284, 378, 413
MboI GATC 5 cut(s) 115, 271, 282, 376, 411
MboII GAAGA 1 cut(s) 233
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 1 cut(s) 112
MluCI AATT 2 cut(s) 451, 492
Mly113I GGCGCC 1 cut(s) 8
MlyI GAGTC 1 cut(s) 240
MroXI GAANNNNTTC 1 cut(s) 198
MseI TTAA 1 cut(s) 261
MspI CCGG 2 cut(s) 159, 398
NarI GGCGCC 1 cut(s) 8
NdeII GATC 5 cut(s) 115, 271, 282, 376, 411
NlaIII CATG 4 cut(s) 171, 251, 289, 316
NlaIV GGNNCC 1 cut(s) 9
NmeAIII GCCGAG 2 cut(s) 111, 453
NmuCI GTSAC 3 cut(s) 163, 325, 445
NspI RCATGY 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 111
PagI TCATGA 1 cut(s) 285
PceI AGGCCT 1 cut(s) 198
PciI ACATGT 1 cut(s) 167
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 1 cut(s) 513
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 239
PluTI GGCGCC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 239
PscI ACATGT 1 cut(s) 167
PshAI GACNNNNGTC 1 cut(s) 125
PspN4I GGNNCC 1 cut(s) 9
PspPI GGNCC 3 cut(s) 63, 156, 395
PsuI RGATCY 1 cut(s) 115
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 5 cut(s) 115, 271, 282, 376, 411
Sau96I GGNCC 3 cut(s) 63, 156, 395
SchI GAGTC 1 cut(s) 240
SduI GDGCHC 1 cut(s) 112
SetI ASST 6 cut(s) 91, 125, 158, 350, 434, 479
SfaNI GCATC 2 cut(s) 178, 267
SfoI GGCGCC 1 cut(s) 9
Sfr274I CTCGAG 1 cut(s) 111
SinI GGWCC 3 cut(s) 63, 156, 395
SlaI CTCGAG 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 111
SmoI CTYRAG 1 cut(s) 111
Sse9I AATT 2 cut(s) 451, 492
SseBI AGGCCT 1 cut(s) 198
SsiI CCGC 2 cut(s) 5, 191
SspDI GGCGCC 1 cut(s) 7
StuI AGGCCT 1 cut(s) 198
TaaI ACNGT 1 cut(s) 508
TaqI TCGA 4 cut(s) 112, 402, 414, 501
TasI AATT 2 cut(s) 451, 492
TatI WGTACW 1 cut(s) 435
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 513
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TseFI GTSAC 3 cut(s) 163, 325, 445
Tsp45I GTSAC 3 cut(s) 163, 325, 445
TspDTI ATGAA 2 cut(s) 231, 384
TspGWI ACGGA 2 cut(s) 95, 164
VpaK11BI GGWCC 3 cut(s) 63, 156, 395
XceI RCATGY 1 cut(s) 171
XcmI CCANNNNNNNNNTGG 1 cut(s) 319
XhoI CTCGAG 1 cut(s) 111
XmnI GAANNNNTTC 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.