Rh2CG027000

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002033
Physical Location & Seq
Forward (+)
2064699 .. 2066192
1494 bp
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UTR
Exon/CDS
Intron
Rh2CG027000.1

Sequence Viewer

Length: 846 bp
ATGAGTGTCCAAGAGATGAGCATCAAAGGTGAGGAACCTCCCCCTCAGTATATTGTTAAAGAAAGCAAGTTTGGAGCTATAGAGTCTACCCCAGAATTGGGTCAAATCCCCATCATTGATGTCAGTCTCTTCTCCCCATCATCAATTGATTCTAACCAAGCGAAGTTCGAACTAGACAAACTTAGAACAGCTCTCAGCTCATCAGGCTGCATCCAGACTAGTGTTCATCAAGTGACCATGCGGTTGGTCATGGGATATCAAGTTCCTTTCTGGACAAGAGAGGTTGTACATGAATATGCAACAAAGGTAAAGTTCATGATGGGTGTTCTTTTTAAGGCCATGGCAAAATCGTTGAATTTGGAAGAGAACGGCTTTTCAGATAAGCTGCTTGGAGAGAGAGCTGTGATGCAAGCAAGATTCAACTTCTATCCGCGGTGTTCAAGATGCGATCAGGTTCTTGGTGTGAAGCCACATACGGATAGGTCAGGAGTGACAGTTTTGTTGCAAGACAAAGAAGTTGAAGGTCTTCAAGTTTTGGTAGATGGCAAATGGGTTAGAGTCCCCATCGTGCCTCATGCTATAGTTGTTAATCTTGGAGATCAAATGCAGATTATGAGTAATGGTGTGTTCAAGAGCCCAATGCACAGGGTGGTGACAAATCCAGAAAGGATGAGGCTATCTGTGGCCTTGTTTAATGAACCAGATCCTGAAACTGAGATTAGTCCAGTGGAGAAACTGATAGATGAGACAAGGCCAAGAGTATACAAAAATGTCAAGAATTATGGTCGCATCAACTACGAATGCTATCAGAGAGGAGAAATAGCACTCGAAACAGTGAAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

281

Amino Acids

31.82

Weight (kDa)

6.97

Isoelectric Point (pI)

35.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 139 - 234 1.1e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 86, 762
AccII CGCG 1 cut(s) 433
AciI CCGC 3 cut(s) 241, 431, 433
AclWI GGATC 1 cut(s) 698
AcsI RAATTY 1 cut(s) 355
AdeI CACNNNGTG 1 cut(s) 649
AfaI GTAC 1 cut(s) 288
AfiI CCNNNNNNNGG 2 cut(s) 97, 98
AgsI TTSAA 6 cut(s) 355, 421, 441, 521, 530, 631
AhlI ACTAGT 1 cut(s) 218
AjuI GAANNNNNNNTTGG 2 cut(s) 54, 86
AloI GAACNNNNNNTCC 2 cut(s) 246, 278
AluBI AGCT 5 cut(s) 77, 191, 198, 385, 401
AluI AGCT 5 cut(s) 77, 191, 198, 385, 401
Alw26I GTCTC 2 cut(s) 131, 740
AlwI GGATC 1 cut(s) 698
AlwNI CAGNNNCTG 1 cut(s) 707
AoxI GGCC 3 cut(s) 336, 684, 752
ApeKI GCWGC 2 cut(s) 207, 385
ApoI RAATTY 1 cut(s) 355
Asp700I GAANNNNTTC 1 cut(s) 525
AsuHPI GGTGA 2 cut(s) 41, 664
AsuII TTCGAA 1 cut(s) 168
BanII GRGCYC 1 cut(s) 638
BbsI GAAGAC 1 cut(s) 518
BbvI GCAGC 2 cut(s) 194, 372
BccI CCATC 5 cut(s) 119, 145, 313, 536, 572
BceAI ACGGC 1 cut(s) 385
BcoDI GTCTC 2 cut(s) 131, 740
BcuI ACTAGT 1 cut(s) 218
BfaI CTAG 2 cut(s) 173, 219
BfmI CTRYAG 2 cut(s) 78, 579
BglII AGATCT 1 cut(s) 840
BisI GCNGC 2 cut(s) 208, 386
BlsI GCNGC 2 cut(s) 209, 387
BmiI GGNNCC 1 cut(s) 36
BmsI GCATC 5 cut(s) 30, 219, 396, 434, 798
BpiI GAAGAC 1 cut(s) 518
Bpu14I TTCGAA 1 cut(s) 168
BsaBI GATNNNNATC 1 cut(s) 20
BsaJI CCNNGG 2 cut(s) 339, 431
BsaXI ACNNNNNCTCC 2 cut(s) 480, 510
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 98
Bse1I ACTGG 1 cut(s) 725
Bse8I GATNNNNATC 1 cut(s) 20
BseDI CCNNGG 2 cut(s) 339, 431
BseGI GGATG 2 cut(s) 210, 675
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 2 cut(s) 97, 98
BseMII CTCAG 3 cut(s) 59, 208, 705
BseNI ACTGG 1 cut(s) 725
BseRI GAGGAG 1 cut(s) 828
BseXI GCAGC 2 cut(s) 194, 372
Bsh1236I CGCG 1 cut(s) 433
BshFI GGCC 3 cut(s) 338, 686, 754
BslFI GGGAC 1 cut(s) 545
BslI CCNNNNNNNGG 2 cut(s) 97, 98
BsmAI GTCTC 2 cut(s) 131, 740
BsmFI GGGAC 1 cut(s) 545
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 3 cut(s) 338, 686, 754
Bsp119I TTCGAA 1 cut(s) 168
Bsp1286I GDGCHC 1 cut(s) 638
Bsp1407I TGTACA 1 cut(s) 286
Bsp143I GATC 4 cut(s) 448, 598, 703, 840
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 3 cut(s) 241, 431, 433
BspANI GGCC 3 cut(s) 338, 686, 754
BspCNI CTCAG 3 cut(s) 58, 207, 706
BspFNI CGCG 1 cut(s) 433
BspHI TCATGA 1 cut(s) 315
BspLI GGNNCC 1 cut(s) 36
BspPI GGATC 1 cut(s) 698
BspT104I TTCGAA 1 cut(s) 168
BsrGI TGTACA 1 cut(s) 286
BsrI ACTGG 1 cut(s) 725
BssECI CCNNGG 2 cut(s) 339, 431
BssMI GATC 4 cut(s) 448, 598, 703, 840
BssNAI GTATAC 1 cut(s) 763
BssT1I CCWWGG 1 cut(s) 339
Bst1107I GTATAC 1 cut(s) 763
Bst4CI ACNGT 2 cut(s) 496, 835
Bst6I CTCTTC 2 cut(s) 134, 357
BstAUI TGTACA 1 cut(s) 286
BstBI TTCGAA 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 411
BstDEI CTNAG 4 cut(s) 45, 182, 194, 714
BstDSI CCRYGG 2 cut(s) 339, 431
BstF5I GGATG 2 cut(s) 210, 675
BstFNI CGCG 1 cut(s) 433
BstKTI GATC 4 cut(s) 451, 601, 706, 843
BstMAI GTCTC 2 cut(s) 131, 740
BstMBI GATC 4 cut(s) 448, 598, 703, 840
BstMWI GCNNNNNNNGC 1 cut(s) 204
BstSFI CTRYAG 2 cut(s) 78, 579
BstUI CGCG 1 cut(s) 433
BstV1I GCAGC 2 cut(s) 194, 372
BstV2I GAAGAC 1 cut(s) 518
BstX2I RGATCY 2 cut(s) 703, 840
BstXI CCANNNNNNTGG 1 cut(s) 244
BstYI RGATCY 2 cut(s) 703, 840
BstZ17I GTATAC 1 cut(s) 763
BsuRI GGCC 3 cut(s) 338, 686, 754
BtgI CCRYGG 2 cut(s) 339, 431
BtsCI GGATG 2 cut(s) 210, 675
BtsIMutI CAGTG 2 cut(s) 732, 840
Cac8I GCNNGC 1 cut(s) 411
CaiI CAGNNNCTG 1 cut(s) 707
CciI TCATGA 1 cut(s) 315
Cfr42I CCGCGG 1 cut(s) 434
Csp6I GTAC 1 cut(s) 287
CviAII CATG 6 cut(s) 238, 250, 290, 316, 340, 575
CviQI GTAC 1 cut(s) 287
DdeI CTNAG 4 cut(s) 45, 182, 194, 714
DpnI GATC 4 cut(s) 450, 600, 705, 842
DpnII GATC 4 cut(s) 448, 598, 703, 840
DraIII CACNNNGTG 1 cut(s) 649
Eam1104I CTCTTC 2 cut(s) 134, 357
EarI CTCTTC 2 cut(s) 134, 357
Eco130I CCWWGG 1 cut(s) 339
Eco24I GRGCYC 1 cut(s) 638
Eco32I GATATC 1 cut(s) 257
EcoRV GATATC 1 cut(s) 257
EcoT14I CCWWGG 1 cut(s) 339
EcoT38I GRGCYC 1 cut(s) 638
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 6 cut(s) 241, 253, 293, 319, 343, 578
FaqI GGGAC 1 cut(s) 545
FatI CATG 6 cut(s) 237, 249, 289, 315, 339, 574
FblI GTMKAC 2 cut(s) 86, 762
Fnu4HI GCNGC 2 cut(s) 208, 386
FokI GGATG 2 cut(s) 197, 682
FriOI GRGCYC 1 cut(s) 638
Fsp4HI GCNGC 2 cut(s) 208, 386
FspBI CTAG 2 cut(s) 173, 219
GluI GCNGC 2 cut(s) 208, 386
HaeIII GGCC 3 cut(s) 338, 686, 754
Hin1II CATG 6 cut(s) 241, 253, 293, 319, 343, 578
HinfI GANTC 4 cut(s) 83, 149, 417, 558
HphI GGTGA 2 cut(s) 41, 664
Hpy166II GTNNAC 2 cut(s) 87, 763
Hpy188I TCNGA 2 cut(s) 379, 810
Hpy188III TCNNGA 9 cut(s) 214, 271, 316, 441, 486, 631, 662, 707, 775
Hpy8I GTNNAC 2 cut(s) 87, 763
HpyAV CCTTC 1 cut(s) 515
HpyCH4III ACNGT 2 cut(s) 496, 835
HpyCH4V TGCA 6 cut(s) 210, 299, 409, 505, 607, 643
HpyF10VI GCNNNNNNNGC 1 cut(s) 204
HpyF3I CTNAG 4 cut(s) 45, 182, 194, 714
Hsp92II CATG 6 cut(s) 241, 253, 293, 319, 343, 578
KspI CCGCGG 1 cut(s) 434
Kzo9I GATC 4 cut(s) 448, 598, 703, 840
LmnI GCTCC 1 cut(s) 74
Lsp1109I GCAGC 2 cut(s) 194, 372
LweI GCATC 5 cut(s) 30, 219, 396, 434, 798
MaeI CTAG 2 cut(s) 173, 219
MaeIII GTNAC 3 cut(s) 232, 490, 652
MalI GATC 4 cut(s) 450, 600, 705, 842
MboI GATC 4 cut(s) 448, 598, 703, 840
MboII GAAGA 3 cut(s) 121, 374, 518
MfeI CAATTG 1 cut(s) 144
MflI RGATCY 2 cut(s) 703, 840
MhlI GDGCHC 1 cut(s) 638
MluCI AATT 4 cut(s) 95, 144, 355, 778
MlyI GAGTC 2 cut(s) 92, 567
MnlI CCTC 7 cut(s) 25, 48, 54, 274, 582, 666, 806
MroXI GAANNNNTTC 1 cut(s) 525
MseI TTAA 4 cut(s) 57, 333, 588, 693
MslI CAYNNNNRTG 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 433
MunI CAATTG 1 cut(s) 144
Mva1269I GAATGC 1 cut(s) 806
MvnI CGCG 1 cut(s) 433
MwoI GCNNNNNNNGC 1 cut(s) 204
NcoI CCATGG 1 cut(s) 339
NdeII GATC 4 cut(s) 448, 598, 703, 840
NlaIII CATG 6 cut(s) 241, 253, 293, 319, 343, 578
NlaIV GGNNCC 1 cut(s) 36
NmuCI GTSAC 3 cut(s) 232, 490, 652
NspV TTCGAA 1 cut(s) 168
PagI TCATGA 1 cut(s) 315
PctI GAATGC 1 cut(s) 806
PdmI GAANNNNTTC 1 cut(s) 525
PfeI GAWTC 2 cut(s) 149, 417
PkrI GCNGC 2 cut(s) 209, 387
PleI GAGTC 2 cut(s) 91, 566
PpsI GAGTC 2 cut(s) 91, 566
PspN4I GGNNCC 1 cut(s) 36
PstNI CAGNNNCTG 1 cut(s) 707
PsuI RGATCY 2 cut(s) 703, 840
RsaI GTAC 1 cut(s) 288
RsaNI GTAC 1 cut(s) 287
RseI CAYNNNNRTG 1 cut(s) 294
SacII CCGCGG 1 cut(s) 434
SaqAI TTAA 4 cut(s) 57, 333, 588, 693
SatI GCNGC 2 cut(s) 208, 386
Sau3AI GATC 4 cut(s) 448, 598, 703, 840
SchI GAGTC 2 cut(s) 92, 567
SduI GDGCHC 1 cut(s) 638
SfaNI GCATC 5 cut(s) 30, 219, 396, 434, 798
SfcI CTRYAG 2 cut(s) 78, 579
Sfr303I CCGCGG 1 cut(s) 434
SfuI TTCGAA 1 cut(s) 168
SgrBI CCGCGG 1 cut(s) 434
SmiMI CAYNNNNRTG 1 cut(s) 294
SpeI ACTAGT 1 cut(s) 218
Sse9I AATT 4 cut(s) 95, 144, 355, 778
SsiI CCGC 3 cut(s) 241, 431, 433
SspMI CTAG 2 cut(s) 173, 219
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 2 cut(s) 496, 835
TaqI TCGA 2 cut(s) 168, 828
TasI AATT 4 cut(s) 95, 144, 355, 778
TatI WGTACW 1 cut(s) 286
TfiI GAWTC 2 cut(s) 149, 417
Tru1I TTAA 4 cut(s) 57, 333, 588, 693
Tru9I TTAA 4 cut(s) 57, 333, 588, 693
TscAI CASTG 2 cut(s) 732, 840
TseFI GTSAC 3 cut(s) 232, 490, 652
TseI GCWGC 2 cut(s) 207, 385
Tsp45I GTSAC 3 cut(s) 232, 490, 652
TspDTI ATGAA 4 cut(s) 215, 304, 306, 711
TspGWI ACGGA 1 cut(s) 491
TspRI CASTG 2 cut(s) 732, 840
XapI RAATTY 1 cut(s) 355
XmiI GTMKAC 2 cut(s) 86, 762
XmnI GAANNNNTTC 1 cut(s) 525
XspI CTAG 2 cut(s) 173, 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.