RLG00000015847

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002033
Physical Location & Seq
Forward (+)
1861568 .. 1862132
565 bp
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UTR
Exon/CDS
Intron
RLM00000015847

Sequence Viewer

Length: 468 bp
ATGGGTGTTCTGTTTAAGGCCATGGCGAAATCGTTGACTTTGGAAGAAACAACTTTTTTTAATAAGCTGCTTGGAGAGATATCTCTGATGCAAGCAAGATTCAACTTTTATTCCGCGGTGTTCAAAAGCCGATCAGGTTCTTGGTGTGAAGGCACACATAGACAGGACAAAGAAGTAGAGGGTCTTCAAGTTTTGGTGGATGGTAAATGGGTTACAGTCCCCATTGTGCCACAGGCCATTGTTGTTAATCTTAGTGATCAAGTGCAGATTTTGACACTGTCTGACTTCATACTGCAGATTATGAGTAATGGCATATTCAAGGGCCCACTGCACAGGGTGATGAAGCTATCTGTGGCCTTGCTTAATGAACCAGATGCTGATACTGAGATTGGTCCAGTGGAGAAACTGATAGATGAGACAAGGCCAAGGTCATACAAAAATGTCAAAAATTATGGCAATATCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

156

Amino Acids

17.43

Weight (kDa)

7.83

Isoelectric Point (pI)

31.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 55 - 114 7e-11 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 116
AciI CCGC 2 cut(s) 114, 116
AdeI CACNNNGTG 1 cut(s) 337
AgsI TTSAA 4 cut(s) 103, 124, 188, 319
AluBI AGCT 2 cut(s) 67, 346
AluI AGCT 2 cut(s) 67, 346
Alw26I GTCTC 1 cut(s) 410
AlwNI CAGNNNCTG 1 cut(s) 377
AoxI GGCC 5 cut(s) 18, 234, 322, 354, 422
ApaI GGGCCC 1 cut(s) 326
ApeKI GCWGC 1 cut(s) 67
AspS9I GGNCC 3 cut(s) 322, 323, 392
AsuHPI GGTGA 1 cut(s) 349
AvaII GGWCC 1 cut(s) 392
BaeGI GKGCMC 1 cut(s) 326
BanII GRGCYC 1 cut(s) 326
BarI GAAGNNNNNNTAC 2 cut(s) 168, 200
BbsI GAAGAC 1 cut(s) 176
BbvI GCAGC 1 cut(s) 54
BccI CCATC 1 cut(s) 194
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 1 cut(s) 410
BfmI CTRYAG 1 cut(s) 293
BisI GCNGC 1 cut(s) 68
BlsI GCNGC 1 cut(s) 69
Bme18I GGWCC 1 cut(s) 392
BmgT120I GGNCC 3 cut(s) 322, 323, 392
BmiI GGNNCC 1 cut(s) 324
BmsI GCATC 2 cut(s) 78, 364
BpiI GAAGAC 1 cut(s) 176
BsaJI CCNNGG 3 cut(s) 21, 114, 425
Bse1I ACTGG 1 cut(s) 395
BseDI CCNNGG 3 cut(s) 21, 114, 425
BseGI GGATG 1 cut(s) 205
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 1 cut(s) 395
BseSI GKGCMC 1 cut(s) 326
BseXI GCAGC 1 cut(s) 54
BsgI GTGCAG 2 cut(s) 284, 314
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 5 cut(s) 20, 236, 324, 356, 424
BslFI GGGAC 1 cut(s) 203
BsmAI GTCTC 1 cut(s) 410
BsmFI GGGAC 1 cut(s) 203
BsnI GGCC 5 cut(s) 20, 236, 324, 356, 424
Bsp120I GGGCCC 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 326
Bsp143I GATC 2 cut(s) 131, 256
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 2 cut(s) 114, 116
BspANI GGCC 5 cut(s) 20, 236, 324, 356, 424
BspCNI CTCAG 1 cut(s) 376
BspFNI CGCG 1 cut(s) 116
BspLI GGNNCC 1 cut(s) 324
BspMAI CTGCAG 1 cut(s) 297
BsrI ACTGG 1 cut(s) 395
BssECI CCNNGG 3 cut(s) 21, 114, 425
BssMI GATC 2 cut(s) 131, 256
BssT1I CCWWGG 2 cut(s) 21, 425
Bst4CI ACNGT 2 cut(s) 217, 279
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 2 cut(s) 251, 384
BstDSI CCRYGG 2 cut(s) 21, 114
BstF5I GGATG 1 cut(s) 205
BstFNI CGCG 1 cut(s) 116
BstKTI GATC 2 cut(s) 134, 259
BstMAI GTCTC 1 cut(s) 410
BstMBI GATC 2 cut(s) 131, 256
BstSFI CTRYAG 1 cut(s) 293
BstSLI GKGCMC 1 cut(s) 326
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 1 cut(s) 54
BstV2I GAAGAC 1 cut(s) 176
BsuRI GGCC 5 cut(s) 20, 236, 324, 356, 424
BtgI CCRYGG 2 cut(s) 21, 114
BtsCI GGATG 1 cut(s) 205
BtsI GCAGTG 1 cut(s) 326
BtsIMutI CAGTG 3 cut(s) 275, 326, 402
Cac8I GCNNGC 1 cut(s) 93
CaiI CAGNNNCTG 1 cut(s) 377
Cfr13I GGNCC 3 cut(s) 322, 323, 392
Cfr42I CCGCGG 1 cut(s) 117
CviAII CATG 1 cut(s) 22
CviJI RGCY 8 cut(s) 20, 67, 129, 236, 324, 346, 356, 424
CviKI_1 RGCY 8 cut(s) 20, 67, 129, 236, 324, 346, 356, 424
DdeI CTNAG 2 cut(s) 251, 384
DpnI GATC 2 cut(s) 133, 258
DpnII GATC 2 cut(s) 131, 256
DraIII CACNNNGTG 1 cut(s) 337
Eco130I CCWWGG 2 cut(s) 21, 425
Eco24I GRGCYC 1 cut(s) 326
Eco32I GATATC 1 cut(s) 81
Eco47I GGWCC 1 cut(s) 392
EcoO109I RGGNCCY 1 cut(s) 322
EcoRV GATATC 1 cut(s) 81
EcoT14I CCWWGG 2 cut(s) 21, 425
EcoT38I GRGCYC 1 cut(s) 326
ErhI CCWWGG 2 cut(s) 21, 425
FaeI CATG 1 cut(s) 25
FaiI YATR 7 cut(s) 23, 159, 290, 302, 314, 433, 453
FaqI GGGAC 1 cut(s) 203
FatI CATG 1 cut(s) 21
FbaI TGATCA 1 cut(s) 256
Fnu4HI GCNGC 1 cut(s) 68
FokI GGATG 1 cut(s) 212
FriOI GRGCYC 1 cut(s) 326
Fsp4HI GCNGC 1 cut(s) 68
GluI GCNGC 1 cut(s) 68
HaeIII GGCC 5 cut(s) 20, 236, 324, 356, 424
Hin1II CATG 1 cut(s) 25
HincII GTYRAC 1 cut(s) 36
HindII GTYRAC 1 cut(s) 36
HinfI GANTC 1 cut(s) 99
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 1 cut(s) 36
Hpy188I TCNGA 2 cut(s) 87, 283
Hpy8I GTNNAC 1 cut(s) 36
HpyAV CCTTC 1 cut(s) 143
HpyCH4III ACNGT 2 cut(s) 217, 279
HpyCH4V TGCA 4 cut(s) 91, 265, 295, 331
HpyF3I CTNAG 2 cut(s) 251, 384
Hsp92II CATG 1 cut(s) 25
Ksp22I TGATCA 1 cut(s) 256
KspI CCGCGG 1 cut(s) 117
Kzo9I GATC 2 cut(s) 131, 256
LpnPI CCDG 6 cut(s) 120, 149, 218, 319, 384, 408
Lsp1109I GCAGC 1 cut(s) 54
LweI GCATC 2 cut(s) 78, 364
MaeIII GTNAC 1 cut(s) 211
MalI GATC 2 cut(s) 133, 258
MboI GATC 2 cut(s) 131, 256
MboII GAAGA 2 cut(s) 56, 176
MhlI GDGCHC 1 cut(s) 326
MluCI AATT 1 cut(s) 448
MnlI CCTC 1 cut(s) 172
MseI TTAA 4 cut(s) 15, 60, 246, 363
MspA1I CMGCKG 1 cut(s) 116
MvnI CGCG 1 cut(s) 116
NcoI CCATGG 1 cut(s) 21
NdeII GATC 2 cut(s) 131, 256
NlaIII CATG 1 cut(s) 25
NlaIV GGNNCC 1 cut(s) 324
PfeI GAWTC 1 cut(s) 99
PflFI GACNNNGTC 1 cut(s) 277
PkrI GCNGC 1 cut(s) 69
PspN4I GGNNCC 1 cut(s) 324
PspOMI GGGCCC 1 cut(s) 322
PspPI GGNCC 3 cut(s) 322, 323, 392
PstI CTGCAG 1 cut(s) 297
PstNI CAGNNNCTG 1 cut(s) 377
PsyI GACNNNGTC 1 cut(s) 277
SacII CCGCGG 1 cut(s) 117
SaqAI TTAA 4 cut(s) 15, 60, 246, 363
SatI GCNGC 1 cut(s) 68
Sau3AI GATC 2 cut(s) 131, 256
Sau96I GGNCC 3 cut(s) 322, 323, 392
SduI GDGCHC 1 cut(s) 326
SetI ASST 4 cut(s) 69, 139, 348, 431
SfaNI GCATC 2 cut(s) 78, 364
SfcI CTRYAG 1 cut(s) 293
Sfr303I CCGCGG 1 cut(s) 117
SgrBI CCGCGG 1 cut(s) 117
SinI GGWCC 1 cut(s) 392
Sse9I AATT 1 cut(s) 448
SsiI CCGC 2 cut(s) 114, 116
StyI CCWWGG 2 cut(s) 21, 425
TaaI ACNGT 2 cut(s) 217, 279
TasI AATT 1 cut(s) 448
TfiI GAWTC 1 cut(s) 99
Tru1I TTAA 4 cut(s) 15, 60, 246, 363
Tru9I TTAA 4 cut(s) 15, 60, 246, 363
TscAI CASTG 3 cut(s) 282, 333, 402
TseI GCWGC 1 cut(s) 67
TspDTI ATGAA 3 cut(s) 277, 356, 381
TspRI CASTG 3 cut(s) 282, 333, 402
Tth111I GACNNNGTC 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.