Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001236

Physical Location & Seq

Genomic Coordinates Forward (+)

19285220 .. 19294392

Gene Structure 9173 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1198100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3738 bp

ATGGTGTTGGAGTCGCAGGACGAGTTCATGGCCGACGGCTCTGACTCGGGGCCCGGAGCATCCAGGGGTCCCAGGCTGTTTATCAGAGAAATGGTCATGAGGAACTTCAAGTCCTATGCCGGCGAACAACGCGTCGGTCCTTTCCACAAGAGCTTTTCTGCAGTCGTTGGACCAAATGGGAGTGGGAAAAGCAATGTGATAGATGCCATGCTCTTTGTATTTGGAAAGAGAGCTAAGCAGATGCGACTTAACAAAGTATCGGAGCTTATTCACAACTCCACTAATCACCAGAATCTGGATAGTGCTGGAGTTTCTGTTCATTTCCAGGAGATCATTGATTTGGATGATGGTACATTTGAAGCTGTTCCAGGAAGTGATTTTGTAATTACTAGGGTAGCGTTTCGTGATAACTCCTCCAAATATTATATTAATGACCGTGCAAGCAATTTCACGGAAGTTACCAAGAAATTGAAAGGAAAAGGAGTTGACCTGGACAACAATCGTTTTCTGATTCTTCAGGGTGAAGTTGAGCAGATTTCACTTATGAAGCCAAAAGCTCAAGGATCCCATGATGAAGGTTTTCTCGAATACCTTGAAGACATTATCGGAACTAACATATATGTTGAAAAGATTGATGAGTCGTTCAAAGAGTTAGAATCCCTAAATGAAAAAAGGTCCGGTGTGGTGCAAATGGTCAAGTTGGCAGAGAAAGAAAGAGATGGCTTGGAGGATGTGAAGAATGAAGCAGAAGCCTACATGCTGAAAGAGTTGTCACTTTTGAAATGGCAAGAGAAAGCCACAAAGTTGGCTCATGAAGATACCAGTGCAAAAATGGTTGATCTACATGGAAATATGTCCACCTTAGAAGGCAATCTAAAAACTGAAAGGGAGAAGATCCAGGAAAGTAATGATGCATTGAAGGAGCTTGAATCTGTGCACAATAAACACATGAAACAACAAGAGGAACTTGATAACAACCTCAGAAAATGTAAGGAAGAATTTAAGCAGTTTGAAAGGGAGGATGTTAAATATCGGGAGGATCTGAAGCATGTGAAGCAGAAGATCAAGAAGCTCAAGGATAAACTTGAAAAGGATTCAGTAAAGATTAATGAAATTGAGAAGGAGTGTGAGGACTCGACTAATCTGATCCCTGAACTTGAGAAAAGTATTCCAGAACTGCAAAAACTCTTAGCGGATGAAGAAAAAGTCTTGGACGGAATTGCAGAAAATTCAAAAGTGGAAACTGAAAGATACCGCTCTGAGCTTACGAAGGTTCGTGCTCAATTAGAACCATGGGAAAAGCAACTGATCGAGCGCAAGGGGAAACTTGAAGTTGCATCTACTGAACAGAACCTTTTAAATGAAAAGCACGAAGCTGGTCGTGCAGCTTTTGAAGATGCTCAAAAGCAGATGGCTGATATATCAGGAAGTATTGAAACAAAAAGCGCAGGCATTGCAAAAGTTCAAAGTGAAATAGAAAAGAGCAAGCTTGAAGGGATCGAAGCTCGTAAAGAGGAACAGGAATACATTAGGGAACAGGAAGCTCTGATTCCTTCTGAACAAGCTGCAAGACAGAAGGTTGCAGAACTTAAGTCGGTTTTAGATTCTGAAAGGAATCAAGGAACTGTTTTAAAAGCAATTTTACATGCTAAGGAGTCCAACCGAATACAGGGAATACATGGCCGGATGGGTGATTTAGGTGCTATTGATGCAAAATATGATGTTGCCATATCAACAGCTTGCGCTGGACTTGATTATATTGTGGTGGAAACAACTAGTGCAGCCCAAGCATGTGTTGAGCTACTCCGAAGGGAGAATCTTGGCGTTGCAACTTTCATGATATTGGAAAAGCAAGGTGATCTTTTACCAAAGCTGAAGGAGAATGTAAACACTCCGGAAGGAGTTCCACGCCTTTTTGATCTAATTAGAGTTCAAGATGAGAGAATGAAGCTCGCATTCTTTGCAGCTTTGGGAAACACAATTGTAGCTAAAGACCTTGATCAGGCAACACGTATTGCATATGGCGGAAACAGAGAATTTCGACGAGTGGTGACGCTTGATGGTGCTTTATTCGAAAAATCTGGTACTATGAGTGGCGGAGGAAGCAAGCCACGTGGTGGAAAGATGAGCACTTCTATTCGTGCAACTAGTGTTTCAGGAGAAGCTGTTGCAAATGCTGAGAAGGAGCTTGCAGCCATGGTTGATTCATTAAATACCATCCGCCAAAAAATTGCCGATGCAGTGCGGAGGTATCAAGCCTCAGAAAAAGCAGTTGCACGCTTGGAGATGGAATTAGCTAAAAGTCAGAAAGAGATTGACAGTTTGAATTCACAACATTGTTATCTTGAGAAACAAATTGGTTCCCTGGAGGCTGCTTCACAGCCAAAGAAGGATGAGCTTGATCGCCTAGCTGAACTCAAGCATCTTATTTCTGTTGAAGAAAAGGAGATCGATAAACTTATACAAGGTTCAAAGCAGCTGAAGGAGAAGGCCTCAGAGCTCCAGAGTAATATAGAAAATGCTGGTGGCGAAAGACTAAAAGCACAGAATCTAAAGGTCAATAAGATTCAGTCTGATATTGATAAAAACAATACTGAGATTAACCGCCGCAAAGTCCAGATTGAGACTGGCCAGAAAACTATAAAGAAGTTGACAAAGACGATTGAAGAATCCAAGACTGAGAAAGAACGACTTGATAAGGAGAAGGAAAATGTGTGTTCCAAATTCAAAGAAATAGAACAGAAGGCATTTGTAGTTAAGGAAAACTACGAGGGAATACAGAAGCTGATTGATGAACATAAAGATATCTTGGGTCAAGCAAAATCTGACTACAACCAAATGAAAGGGACTGTGGATGAACTGCGCGCATCACAGGTTGACGCTGACTTTAAATTACAAGATATGAAGAAGCTATACAAGGAGCTTGAGTTGAAGGGGAAGGGTTACAAGAAAAGGCTTGATGATTTGCAAACTTCCCTTATAAAGCATATGGAACAAATTCAAAAAGATTTAGTGGATCCTGAAAAGCTTCAAGCTACCTTGGCAGATGAAATTCTTAATAACCCTTCTGACCTTAAAAGGGCACTTGAAATGGTAGCACTATTAGAAGCACAATTGAAAGAGATGAATCCAAATCTAGATTCAATTGCAGAGTATCGGAGAAAGGTCTCATTATATAATGAACGAGTCGAGGATCTTAACACTGTCACTCAACAGCGTGATGACATAAAGAGGCAGTATGATGAATGGAGAAAGAAGAGGTTGGACGAATTCATGGCAGGATTCAATGCAATCTCTTTGAAATTAAAGGAAATGTATCAGATGATCACGCTGGGAGGTGATGCAGAACTTGAGCTAGTGGACTCCCTGGATCCTTTCTCTGAAGGCGTTGTTTTCAGCGTCAGACCGCCAAAGAAGAGTTGGAAGAATATTGCGAATTTATCTGGTGGTGAAAAGACTTTGAGCTCATTGGCTCTTATTTTTGCCCTGCACCATTACAAGCCTACCCCTCTTTATGTGATGGATGAAATCGATGCTGCGCTAGACTTCAAGAATGTCTCTATCGTGGGGCATTATATCAAGGACCGAACGAAGGATGCACAGTTTGTCATCATAAGCCTTCGGAACAATATGTTTGAGTTGGCAGATCGACTGGTTGGGATATACAAAACAGATAACTGCACAAAGAGCATCACTATCAATCCAGGGAGTTTCGTGGTATGCGAAAAAGCTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000070 GO:0000278 GO:0000280 GO:0000819 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006323 GO:0006996 GO:0007049 GO:0007059 GO:0007076 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016043 GO:0022402 GO:0022414 GO:0030261 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048285 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051276 GO:0061458 GO:0071103 GO:0071840 GO:0098813 GO:0140014 GO:1903047

KEGG Pathways

Metabolic & Signaling

ko04111 map04111

Pfam Domains

Protein Families

SMC_N SMC_hinge

Protein Analysis

1246

Amino Acids

140.85

Weight (kDa)

5.81

Isoelectric Point (pI)

34.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SMC_N	PF02463	27 - 700	5.7e-30	RecF/RecN/SMC N terminal domain
AAA_15	PF13175	28 - 402	1e-09	AAA ATPase domain
AAA_29	PF13555	30 - 87	7.9e-06	P-loop containing region of AAA domain
AAA_23	PF13476	31 - 248	2.3e-11	AAA domain
SMC_hinge	PF06470	557 - 673	3.8e-22	SMC proteins Flexible Hinge Domain
SMC_N	PF02463	811 - 1228	1.8e-34	RecF/RecN/SMC N terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0001236)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48600 AT5G48600
fragaria_vesca	FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_4g18080 FvH4_5g30910 FvH4_5g30910 FvH4_5g30910 FvH4_6g23280
malus_domestica	MD02G1198100.v1.1
prunus_persica	Prupe.3G267300_v2.0.a1 Prupe.3G267300_v2.0.a1
pyrus_communis	pycom02g16170
rosa_chinensis	RchiOBHm_Chr5g0008421 RchiOBHm_Chr6g0300801 RchiOBHm_Chr7g0223681 RchiOBHm_Chr7g0223741 RchiOBHm_Chr7g0226461
rosa_laevigata	RLG00000001729 RLG00000011300 RLG00000031570
rosa_multiflora	Rmu_sc0001470.1_g000005 Rmu_sc0001903.1_g000024 Rmu_sc0002826.1_g000022 Rmu_sc0008947.1_g000001
rosa_roxburghii	Rroxscaffold_3G00232810 Rroxscaffold_3G00235410 Rroxscaffold_7G00167400
rosa_rugosa	Rorug06G0299200 Rorug07G0218700 Rorug07G0219100 Rorug07G0219200 Rorug07G0237300
rosa_samantha	Rh6AG412200 Rh6BG419400 Rh6CG426900 Rh6DG412200 Rh7AG362000 Rh7AG384400 Rh7AG384500 Rh7BG353400 Rh7BG353500 Rh7BG371100 Rh7CG380600 Rh7CG403700 Rh7CG403800 Rh7DG356900 Rh7DG357400 Rh7DG383200
rosa_wichuraiana	Rw7G030730 Rw7G030770 Rw7G032280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2981
AccB7I	CCANNNNNTGG	2 cut(s)	295, 2117
AccBSI	CCGCTC	1 cut(s)	1257
AccII	CGCG	2 cut(s)	132, 2865
AccIII	TCCGGA	1 cut(s)	1894
AciI	CCGC	9 cut(s)	1193, 1255, 2025, 2097, 2221, 2245, 2605, 2608, 3407
AcoI	YGGCCR	3 cut(s)	30, 1681, 2629
AcsI	RAATTY	9 cut(s)	998, 1228, 2036, 2326, 2723, 2997, 3051, 3269, 3436
AcuI	CTGAAG	5 cut(s)	500, 1064, 1895, 2501, 3402
AcvI	CACGTG	1 cut(s)	2114
AdeI	CACNNNGTG	1 cut(s)	2117
AfaI	GTAC	2 cut(s)	352, 2086
AfiI	CCNNNNNNNGG	6 cut(s)	295, 1669, 2002, 2117, 3079, 3592
AflII	CTTAAG	1 cut(s)	1589
AflIII	ACRYGT	2 cut(s)	130, 2009
AhlI	ACTAGT	2 cut(s)	1775, 2147
AjnI	CCWGG	9 cut(s)	62, 71, 324, 367, 489, 897, 2364, 3366, 3703
AjuI	GAANNNNNNNTTGG	2 cut(s)	3124, 3156
AloI	GAACNNNNNNTCC	4 cut(s)	95, 127, 300, 332
Alw21I	GWGCWC	5 cut(s)	939, 1282, 2132, 2502, 3467
Alw26I	GTCTC	3 cut(s)	2618, 3172, 3562
Alw44I	GTGCAC	1 cut(s)	935
AlwNI	CAGNNNCTG	2 cut(s)	295, 2785
Ama87I	CYCGRG	1 cut(s)	46
Aor13HI	TCCGGA	1 cut(s)	1894
AoxI	GGCC	5 cut(s)	30, 50, 1681, 2490, 2629
ApaI	GGGCCC	1 cut(s)	54
ApaLI	GTGCAC	1 cut(s)	935
ApeKI	GCWGC	9 cut(s)	1385, 1565, 1781, 1964, 2192, 2372, 2476, 3536, 3731
ApoI	RAATTY	9 cut(s)	998, 1228, 2036, 2326, 2723, 2997, 3051, 3269, 3436
ArsI	GACNNNNNNTTYG	2 cut(s)	117, 149
AseI	ATTAAT	2 cut(s)	429, 1107
Asp700I	GAANNNNTTC	5 cut(s)	363, 579, 1902, 2997, 3027
AspLEI	GCGC	6 cut(s)	1317, 1448, 1745, 2865, 2867, 3541
AspS9I	GGNCC	7 cut(s)	50, 51, 68, 137, 170, 675, 3583
AsuC2I	CCSGG	1 cut(s)	54
AsuHPI	GGTGA	7 cut(s)	278, 533, 1703, 1868, 2062, 3350, 3461
AsuII	TTCGAA	1 cut(s)	2073
AvaI	CYCGRG	1 cut(s)	46
AvaII	GGWCC	5 cut(s)	68, 137, 170, 675, 3583
BaeGI	GKGCMC	3 cut(s)	54, 939, 3085
BalI	TGGCCA	1 cut(s)	2631
BamHI	GGATCC	3 cut(s)	563, 3016, 3370
BanII	GRGCYC	3 cut(s)	54, 2502, 3467
BarI	GAAGNNNNNNTAC	2 cut(s)	2897, 2929
BbrPI	CACGTG	1 cut(s)	2114
BbsI	GAAGAC	1 cut(s)	603
Bbv12I	GWGCWC	5 cut(s)	939, 1282, 2132, 2502, 3467
BbvI	GCAGC	9 cut(s)	1397, 1552, 1793, 1976, 2204, 2359, 2488, 3523, 3718
BccI	CCATC	8 cut(s)	341, 713, 1405, 1681, 2054, 2225, 2281, 3514
BceAI	ACGGC	1 cut(s)	52
BcgI	CGANNNNNNTGC	1 cut(s)	3713
BciT130I	CCWGG	9 cut(s)	64, 73, 326, 369, 491, 899, 2366, 3368, 3705
BclI	TGATCA	2 cut(s)	1999, 3324
BcnI	CCSGG	1 cut(s)	54
BcoDI	GTCTC	3 cut(s)	2618, 3172, 3562
BcuI	ACTAGT	2 cut(s)	1775, 2147
BfaI	CTAG	7 cut(s)	390, 1776, 2148, 2408, 3137, 3356, 3542
BfmI	CTRYAG	1 cut(s)	159
BfrI	CTTAAG	1 cut(s)	1589
BlpI	GCTNAGC	1 cut(s)	234
Bme18I	GGWCC	5 cut(s)	68, 137, 170, 675, 3583
BmeT110I	CYCGRG	1 cut(s)	46
BmgT120I	GGNCC	7 cut(s)	50, 51, 68, 137, 170, 675, 3583
BmiI	GGNNCC	8 cut(s)	51, 52, 69, 70, 565, 2362, 3018, 3372
BpiI	GAAGAC	1 cut(s)	603
BplI	GAGNNNNNCTC	2 cut(s)	2477, 2509
BpmI	CTGGAG	3 cut(s)	327, 2387, 2486
Bpu10I	CCTNAGC	1 cut(s)	1650
Bpu1102I	GCTNAGC	1 cut(s)	234
Bpu14I	TTCGAA	1 cut(s)	2073
BpuEI	CTTGAG	7 cut(s)	543, 1058, 1178, 2366, 2402, 2945, 3371
BpuMI	CCSGG	1 cut(s)	54
Bsa29I	ATCGAT	2 cut(s)	2451, 3531
BsaAI	YACGTR	2 cut(s)	2012, 2114
BsaI	GGTCTC	1 cut(s)	3172
BsaJI	CCNNGG	8 cut(s)	63, 71, 1292, 2196, 2364, 3039, 3366, 3704
BsaWI	WCCGGW	2 cut(s)	677, 1894
BsaXI	ACNNNNNCTCC	6 cut(s)	300, 330, 2151, 2181, 2912, 2942
Bsc4I	CCNNNNNNNGG	6 cut(s)	295, 1669, 2002, 2117, 3079, 3592
Bse118I	RCCGGY	1 cut(s)	119
Bse1I	ACTGG	3 cut(s)	822, 2632, 3657
Bse3DI	GCAATG	2 cut(s)	199, 1452
BseAI	TCCGGA	1 cut(s)	1894
BseBI	CCWGG	9 cut(s)	64, 73, 326, 369, 491, 899, 2366, 3368, 3705
BseCI	ATCGAT	2 cut(s)	2451, 3531
BseDI	CCNNGG	8 cut(s)	63, 71, 1292, 2196, 2364, 3039, 3366, 3704
BseLI	CCNNNNNNNGG	6 cut(s)	295, 1669, 2002, 2117, 3079, 3592
BseMI	GCAATG	2 cut(s)	199, 1452
BseMII	CTCAG	7 cut(s)	994, 1251, 2169, 2274, 2508, 2586, 2670
BseNI	ACTGG	3 cut(s)	822, 2632, 3657
BsePI	GCGCGC	1 cut(s)	2863
BseRI	GAGGAG	1 cut(s)	403
BseSI	GKGCMC	3 cut(s)	54, 939, 3085
BseXI	GCAGC	9 cut(s)	1397, 1552, 1793, 1976, 2204, 2359, 2488, 3523, 3718
BseYI	CCCAGC	1 cut(s)	3331
BsgI	GTGCAG	4 cut(s)	1404, 1800, 3473, 3664
Bsh1236I	CGCG	2 cut(s)	132, 2865
BshFI	GGCC	5 cut(s)	32, 52, 1683, 2492, 2631
BshVI	ATCGAT	2 cut(s)	2451, 3531
BsiHKAI	GWGCWC	5 cut(s)	939, 1282, 2132, 2502, 3467
BsiHKCI	CYCGRG	1 cut(s)	46
BsiSI	CCGG	5 cut(s)	54, 120, 678, 1684, 1895
BslFI	GGGAC	2 cut(s)	54, 2860
BslI	CCNNNNNNNGG	6 cut(s)	295, 1669, 2002, 2117, 3079, 3592
BsmAI	GTCTC	3 cut(s)	2618, 3172, 3562
BsmFI	GGGAC	2 cut(s)	54, 2860
BsmI	GAATGC	1 cut(s)	1955
BsnI	GGCC	5 cut(s)	32, 52, 1683, 2492, 2631
Bso31I	GGTCTC	1 cut(s)	3172
BsoBI	CYCGRG	1 cut(s)	46
Bsp119I	TTCGAA	1 cut(s)	2073
Bsp120I	GGGCCC	1 cut(s)	50
Bsp1286I	GDGCHC	7 cut(s)	54, 939, 1282, 2132, 2502, 3085, 3467
Bsp13I	TCCGGA	1 cut(s)	1894
Bsp1720I	GCTNAGC	1 cut(s)	234
Bsp19I	CCATGG	2 cut(s)	1292, 2196
BspACI	CCGC	9 cut(s)	1193, 1255, 2025, 2097, 2221, 2245, 2605, 2608, 3407
BspANI	GGCC	5 cut(s)	32, 52, 1683, 2492, 2631
BspCNI	CTCAG	7 cut(s)	993, 1252, 2170, 2273, 2507, 2587, 2671
BspDI	ATCGAT	2 cut(s)	2451, 3531
BspEI	TCCGGA	1 cut(s)	1894
BspFNI	CGCG	2 cut(s)	132, 2865
BspHI	TCATGA	3 cut(s)	96, 811, 1836
BspLI	GGNNCC	8 cut(s)	51, 52, 69, 70, 565, 2362, 3018, 3372
BspMAI	CTGCAG	1 cut(s)	163
BspT104I	TTCGAA	1 cut(s)	2073
BspTI	CTTAAG	1 cut(s)	1589
BspTNI	GGTCTC	1 cut(s)	3172
BsrBI	CCGCTC	1 cut(s)	1257
BsrDI	GCAATG	2 cut(s)	199, 1452
BsrFI	RCCGGY	1 cut(s)	119
BsrI	ACTGG	3 cut(s)	822, 2632, 3657
BssAI	RCCGGY	1 cut(s)	119
BssECI	CCNNGG	8 cut(s)	63, 71, 1292, 2196, 2364, 3039, 3366, 3704
BssHII	GCGCGC	1 cut(s)	2863
BssT1I	CCWWGG	3 cut(s)	1292, 2196, 3039
Bst2UI	CCWGG	9 cut(s)	64, 73, 326, 369, 491, 899, 2366, 3368, 3705
Bst4CI	ACNGT	6 cut(s)	437, 1627, 2321, 2851, 3205, 3603
Bst6I	CTCTTC	2 cut(s)	3251, 3410
BstAFI	CTTAAG	1 cut(s)	1589
BstAPI	GCANNNNNTGC	2 cut(s)	1454, 1961
BstBAI	YACGTR	2 cut(s)	2012, 2114
BstBI	TTCGAA	1 cut(s)	2073
BstDSI	CCRYGG	2 cut(s)	1292, 2196
BstENI	CCTNNNNNAGG	1 cut(s)	2000
BstFNI	CGCG	2 cut(s)	132, 2865
BstHHI	GCGC	6 cut(s)	1317, 1448, 1745, 2865, 2867, 3541
BstMAI	GTCTC	3 cut(s)	2618, 3172, 3562
BstNI	CCWGG	9 cut(s)	64, 73, 326, 369, 491, 899, 2366, 3368, 3705
BstNSI	RCATGY	4 cut(s)	760, 1052, 1649, 1794
BstSFI	CTRYAG	1 cut(s)	159
BstSLI	GKGCMC	3 cut(s)	54, 939, 3085
BstUI	CGCG	2 cut(s)	132, 2865
BstV1I	GCAGC	9 cut(s)	1397, 1552, 1793, 1976, 2204, 2359, 2488, 3523, 3718
BstV2I	GAAGAC	1 cut(s)	603
BstX2I	RGATCY	6 cut(s)	563, 894, 1039, 3016, 3193, 3370
BstXI	CCANNNNNNTGG	1 cut(s)	805
BstYI	RGATCY	6 cut(s)	563, 894, 1039, 3016, 3193, 3370
Bsu15I	ATCGAT	2 cut(s)	2451, 3531
BsuRI	GGCC	5 cut(s)	32, 52, 1683, 2492, 2631
BsuTUI	ATCGAT	2 cut(s)	2451, 3531
BtgI	CCRYGG	2 cut(s)	1292, 2196
BtsI	GCAGTG	1 cut(s)	2247
BtsIMutI	CAGTG	3 cut(s)	829, 2247, 3201
CaiI	CAGNNNCTG	2 cut(s)	295, 2785
CciI	TCATGA	3 cut(s)	96, 811, 1836
CfoI	GCGC	6 cut(s)	1317, 1448, 1745, 2865, 2867, 3541
Cfr10I	RCCGGY	1 cut(s)	119
Cfr13I	GGNCC	7 cut(s)	50, 51, 68, 137, 170, 675, 3583
ClaI	ATCGAT	2 cut(s)	2451, 3531
CseI	GACGC	4 cut(s)	121, 2062, 2888, 3388
Csp6I	GTAC	2 cut(s)	351, 2085
CspCI	CAANNNNNGTGG	2 cut(s)	2095, 2130
CviQI	GTAC	2 cut(s)	351, 2085
DraI	TTTAAA	3 cut(s)	1359, 1632, 2890
DraIII	CACNNNGTG	1 cut(s)	2117
EaeI	YGGCCR	3 cut(s)	30, 1681, 2629
Eam1104I	CTCTTC	2 cut(s)	3251, 3410
EarI	CTCTTC	2 cut(s)	3251, 3410
EciI	GGCGGA	3 cut(s)	2040, 2112, 2210
Ecl136II	GAGCTC	2 cut(s)	2500, 3465
Eco130I	CCWWGG	3 cut(s)	1292, 2196, 3039
Eco147I	AGGCCT	1 cut(s)	2492
Eco24I	GRGCYC	3 cut(s)	54, 2502, 3467
Eco31I	GGTCTC	1 cut(s)	3172
Eco32I	GATATC	1 cut(s)	2806
Eco47I	GGWCC	5 cut(s)	68, 137, 170, 675, 3583
Eco53kI	GAGCTC	2 cut(s)	2500, 3465
Eco57I	CTGAAG	5 cut(s)	500, 1064, 1895, 2501, 3402
Eco72I	CACGTG	1 cut(s)	2114
Eco88I	CYCGRG	1 cut(s)	46
EcoICRI	GAGCTC	2 cut(s)	2500, 3465
EcoNI	CCTNNNNNAGG	1 cut(s)	2000
EcoO109I	RGGNCCY	2 cut(s)	50, 68
EcoRI	GAATTC	2 cut(s)	2326, 3269
EcoRII	CCWGG	9 cut(s)	62, 71, 324, 367, 489, 897, 2364, 3366, 3703
EcoRV	GATATC	1 cut(s)	2806
EcoT14I	CCWWGG	3 cut(s)	1292, 2196, 3039
EcoT22I	ATGCAT	1 cut(s)	916
EcoT38I	GRGCYC	3 cut(s)	54, 2502, 3467
ErhI	CCWWGG	3 cut(s)	1292, 2196, 3039
FalI	AAGNNNNNCTT	6 cut(s)	951, 983, 1845, 1877, 2676, 2708
FaqI	GGGAC	2 cut(s)	54, 2860
FauNDI	CATATG	2 cut(s)	2020, 2988
FbaI	TGATCA	2 cut(s)	1999, 3324
FriOI	GRGCYC	3 cut(s)	54, 2502, 3467
FspBI	CTAG	7 cut(s)	390, 1776, 2148, 2408, 3137, 3356, 3542
GlaI	GCGC	6 cut(s)	1316, 1447, 1744, 2864, 2866, 3540
GsaI	CCCAGC	1 cut(s)	3335
GsuI	CTGGAG	3 cut(s)	327, 2387, 2486
HaeIII	GGCC	5 cut(s)	32, 52, 1683, 2492, 2631
HapII	CCGG	5 cut(s)	54, 120, 678, 1684, 1895
HgaI	GACGC	4 cut(s)	121, 2062, 2888, 3388
HhaI	GCGC	6 cut(s)	1317, 1448, 1745, 2865, 2867, 3541
Hin6I	GCGC	6 cut(s)	1315, 1446, 1743, 2863, 2865, 3539
HinP1I	GCGC	6 cut(s)	1315, 1446, 1743, 2863, 2865, 3539
HincII	GTYRAC	3 cut(s)	487, 2652, 2878
HindII	GTYRAC	3 cut(s)	487, 2652, 2878
HindIII	AAGCTT	2 cut(s)	1487, 3026
HpaII	CCGG	5 cut(s)	54, 120, 678, 1684, 1895
HphI	GGTGA	7 cut(s)	278, 533, 1703, 1868, 2062, 3350, 3461
Hpy166II	GTNNAC	7 cut(s)	487, 858, 937, 1888, 2652, 2878, 3361
Hpy8I	GTNNAC	7 cut(s)	487, 858, 937, 1888, 2652, 2878, 3361
Hpy99I	CGWCG	3 cut(s)	38, 137, 2046
HpyCH4III	ACNGT	6 cut(s)	437, 1627, 2321, 2851, 3205, 3603
HpyCH4IV	ACGT	2 cut(s)	2011, 2113
HpySE526I	ACGT	2 cut(s)	2011, 2113
HspAI	GCGC	6 cut(s)	1315, 1446, 1743, 2863, 2865, 3539
KflI	GGGWCCC	1 cut(s)	68
Kpn2I	TCCGGA	1 cut(s)	1894
KroI	GCCGGC	1 cut(s)	119
KroNI	GCCGGC	1 cut(s)	121
Ksp22I	TGATCA	2 cut(s)	1999, 3324
LmnI	GCTCC	6 cut(s)	56, 262, 922, 2185, 2505, 2920
Lsp1109I	GCAGC	9 cut(s)	1397, 1552, 1793, 1976, 2204, 2359, 2488, 3523, 3718
MaeI	CTAG	7 cut(s)	390, 1776, 2148, 2408, 3137, 3356, 3542
MaeII	ACGT	2 cut(s)	2011, 2113
MaeIII	GTNAC	5 cut(s)	457, 771, 2050, 2942, 3205
MbiI	CCGCTC	1 cut(s)	1257
MfeI	CAATTG	3 cut(s)	1980, 3113, 3144
MflI	RGATCY	6 cut(s)	563, 894, 1039, 3016, 3193, 3370
MhlI	GDGCHC	7 cut(s)	54, 939, 1282, 2132, 2502, 3085, 3467
MlsI	TGGCCA	1 cut(s)	2631
MluI	ACGCGT	1 cut(s)	130
MluNI	TGGCCA	1 cut(s)	2631
MlyI	GAGTC	7 cut(s)	20, 38, 647, 1127, 1664, 3195, 3356
MmeI	TCCRAC	4 cut(s)	148, 1683, 3243, 3401
Mox20I	TGGCCA	1 cut(s)	2631
Mph1103I	ATGCAT	1 cut(s)	916
MroI	TCCGGA	1 cut(s)	1894
MroNI	GCCGGC	1 cut(s)	119
MroXI	GAANNNNTTC	5 cut(s)	363, 579, 1902, 2997, 3027
MscI	TGGCCA	1 cut(s)	2631
Msp20I	TGGCCA	1 cut(s)	2631
MspA1I	CMGCKG	1 cut(s)	2479
MspCI	CTTAAG	1 cut(s)	1589
MspI	CCGG	5 cut(s)	54, 120, 678, 1684, 1895
MunI	CAATTG	3 cut(s)	1980, 3113, 3144
Mva1269I	GAATGC	1 cut(s)	1955
MvaI	CCWGG	9 cut(s)	64, 73, 326, 369, 491, 899, 2366, 3368, 3705
MvnI	CGCG	2 cut(s)	132, 2865
NaeI	GCCGGC	1 cut(s)	121
NciI	CCSGG	1 cut(s)	54
NcoI	CCATGG	2 cut(s)	1292, 2196
NdeI	CATATG	2 cut(s)	2020, 2988
NgoMIV	GCCGGC	1 cut(s)	119
NlaIV	GGNNCC	8 cut(s)	51, 52, 69, 70, 565, 2362, 3018, 3372
NmuCI	GTSAC	3 cut(s)	771, 2050, 3205
NsiI	ATGCAT	1 cut(s)	916
NspI	RCATGY	4 cut(s)	760, 1052, 1649, 1794
NspV	TTCGAA	1 cut(s)	2073
PagI	TCATGA	3 cut(s)	96, 811, 1836
PauI	GCGCGC	1 cut(s)	2863
PceI	AGGCCT	1 cut(s)	2492
PcsI	WCGNNNNNNNCGW	1 cut(s)	3720
PctI	GAATGC	1 cut(s)	1955
PdiI	GCCGGC	1 cut(s)	121
PdmI	GAANNNNTTC	5 cut(s)	363, 579, 1902, 2997, 3027
PflMI	CCANNNNNTGG	2 cut(s)	295, 2117
PfoI	TCCNGGA	3 cut(s)	324, 367, 897
PleI	GAGTC	7 cut(s)	19, 38, 646, 1127, 1663, 3194, 3356
PmaCI	CACGTG	1 cut(s)	2114
PmlI	CACGTG	1 cut(s)	2114
PpsI	GAGTC	7 cut(s)	19, 38, 646, 1127, 1663, 3194, 3356
Ppu21I	YACGTR	2 cut(s)	2012, 2114
PpuMI	RGGWCCY	1 cut(s)	68
PshBI	ATTAAT	2 cut(s)	429, 1107
PsiI	TTATAA	1 cut(s)	2981
Psp124BI	GAGCTC	2 cut(s)	2502, 3467
Psp5II	RGGWCCY	1 cut(s)	68
Psp6I	CCWGG	9 cut(s)	62, 71, 324, 367, 489, 897, 2364, 3366, 3703
PspCI	CACGTG	1 cut(s)	2114
PspFI	CCCAGC	1 cut(s)	3331
PspGI	CCWGG	9 cut(s)	62, 71, 324, 367, 489, 897, 2364, 3366, 3703
PspN4I	GGNNCC	8 cut(s)	51, 52, 69, 70, 565, 2362, 3018, 3372
PspOMI	GGGCCC	1 cut(s)	50
PspPI	GGNCC	7 cut(s)	50, 51, 68, 137, 170, 675, 3583
PspPPI	RGGWCCY	1 cut(s)	68
PstI	CTGCAG	1 cut(s)	163
PstNI	CAGNNNCTG	2 cut(s)	295, 2785
PsuI	RGATCY	6 cut(s)	563, 894, 1039, 3016, 3193, 3370
PteI	GCGCGC	1 cut(s)	2863
PvuII	CAGCTG	1 cut(s)	2479
RsaI	GTAC	2 cut(s)	352, 2086
RsaNI	GTAC	2 cut(s)	351, 2085
SacI	GAGCTC	2 cut(s)	2502, 3467
Sau96I	GGNCC	7 cut(s)	50, 51, 68, 137, 170, 675, 3583
SchI	GAGTC	7 cut(s)	20, 38, 647, 1127, 1664, 3195, 3356
SduI	GDGCHC	7 cut(s)	54, 939, 1282, 2132, 2502, 3085, 3467
SfcI	CTRYAG	1 cut(s)	159
SfuI	TTCGAA	1 cut(s)	2073
SinI	GGWCC	5 cut(s)	68, 137, 170, 675, 3583
SmlI	CTYRAG	8 cut(s)	558, 1073, 1157, 1589, 2345, 2417, 2924, 3350
SmoI	CTYRAG	8 cut(s)	558, 1073, 1157, 1589, 2345, 2417, 2924, 3350
SpeI	ACTAGT	2 cut(s)	1775, 2147
SseBI	AGGCCT	1 cut(s)	2492
SsiI	CCGC	9 cut(s)	1193, 1255, 2025, 2097, 2221, 2245, 2605, 2608, 3407
SspI	AATATT	2 cut(s)	422, 3430
SspMI	CTAG	7 cut(s)	390, 1776, 2148, 2408, 3137, 3356, 3542
SstI	GAGCTC	2 cut(s)	2502, 3467
StuI	AGGCCT	1 cut(s)	2492
StyI	CCWWGG	3 cut(s)	1292, 2196, 3039
TaaI	ACNGT	6 cut(s)	437, 1627, 2321, 2851, 3205, 3603
TaiI	ACGT	2 cut(s)	2014, 2116
TaqII	GACCGA	2 cut(s)	125, 3600
TauI	GCSGC	1 cut(s)	2610
TscAI	CASTG	3 cut(s)	829, 2247, 3208
TseFI	GTSAC	3 cut(s)	771, 2050, 3205
TseI	GCWGC	9 cut(s)	1385, 1565, 1781, 1964, 2192, 2372, 2476, 3536, 3731
Tsp45I	GTSAC	3 cut(s)	771, 2050, 3205
TspGWI	ACGGA	2 cut(s)	467, 1230
TspRI	CASTG	3 cut(s)	829, 2247, 3208
Van91I	CCANNNNNTGG	2 cut(s)	295, 2117
Vha464I	CTTAAG	1 cut(s)	1589
VneI	GTGCAC	1 cut(s)	935
VpaK11BI	GGWCC	5 cut(s)	68, 137, 170, 675, 3583
VspI	ATTAAT	2 cut(s)	429, 1107
XagI	CCTNNNNNAGG	1 cut(s)	2000
XapI	RAATTY	9 cut(s)	998, 1228, 2036, 2326, 2723, 2997, 3051, 3269, 3436
XbaI	TCTAGA	1 cut(s)	3136
XceI	RCATGY	4 cut(s)	760, 1052, 1649, 1794
XcmI	CCANNNNNNNNNTGG	3 cut(s)	829, 2624, 3417
XmnI	GAANNNNTTC	5 cut(s)	363, 579, 1902, 2997, 3027
XspI	CTAG	7 cut(s)	390, 1776, 2148, 2408, 3137, 3356, 3542
Zsp2I	ATGCAT	1 cut(s)	916

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.