Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0001236

Physical Location & Seq

Genomic Coordinates Reverse (-)

46806093 .. 46815171

Gene Structure 9079 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G032280.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3621 bp

ATGGAATCGCCGCCGGACAAGTTCATGGCCGATGGCTCGGAGTCGAGGAAGCCAAGGCTGTTCATCAGGGAAATGGTTATGAGCAACTTCAAGTCTTACGCCGGTGAACAATGCGTTGGTCCTTTCCACAAGAGCTTTTCTGCTGTTGTTGGACCTAATGGGAGTGGGAAGAGCAACGTCATAGATGCAATGCTCTTTGTATTCGGAAAACGAGCTAAGCAGATGCGACTTAACAGAGTTTCGGAGCTCATTCATAACTCCACTAATTGCCACAATATGGATAGTGCTCGAGTTTCTCTTCATTTCCAGGAGATCATTGATTTGGACGATGGGACGTTTGAAGCTGTTCCAGGAAGTGATTTTGTAATCACTAGGGTAGCGTATTGGGATAACTCCTCCAGGTATTACATCAATGACCGTTCAAGCAATTTTACTCAAGTTACCAAGAAACTGAAAGGGAAAGGAGTTGACCTGGACAACAATCGCTTCTTGATTCTTCAGGGTGAAGTTGAGCAGATCTCACTCATGAAGCCAAAAGCTCAAGGATCTCAGGATGAAGGCCTCCTCGAGTACCTTGAGGACATTATTGGGACCAGCAAATATGTCGAAAAAATTGATGAATCTTATAAAGAGATAGAGTCCCTAAATGAGAAAAGATCTGGTGTCGTGCAAGTGGTTAAGCTGGCAGAGAAAGAAAGAGATGGCTTGGAGGATGGGAAGAATGAAGCGGAAGCGTACATGCTGAAAGAGTTGTCCCTTCTAAATTGGCAAGAGAAAGCCACACAATTGGCTTATGAAGATACCTTTGCAAAAATGCTTGATCTGCAGGAAACTATAACCAGCTTAGAAGAAAATCTGAACAATGACAGGGAGAAGATCCAGGAGCGTCATAATGCAGTGAAGGAGCTTGAATCTGTGTACAATAAACATATGAAACAACAAGAGGAATTGTATAATACCCTCAACAGATATAAGGATGAATTTAAGCATCTTGAAAAGGAGCGTGATTCATTAAAGATTAATGACAGAGAGAGGGAATGTGAGAACTCGACTAATCAGATCCCAGAACTTGAGAAAAGTATTCCCCAACTGCGAAAACTCTTGTTGGATGAAGAAAAAGTCCTGGAGGAAATTATTGAGAATTCCAAAGGGGATACCGAAACATATCGCTGTGAGCTTACAACAGTTCGTTCCAGATTAGAACCTTGGGAAAAACAACTGATTGAGCACCAGAAGAAATTTGATGTTGCATATACTGAACAGAAGCTTTTGGATGAAAAGCATCAAGCTGGTCGTGCAGCTTTTGAAGACGCTCAAAAGCAGATGGATGATATATTGGGAATGATACAAACAAAAACTGCAGACATTGCAAAAATTTGGTGTGAGCTAGAAAAGAGCAAATTCGAAAGGACGGAATACTGTAAAGCAGAACAGGAATACATCAAAGAACAAGAAGCTCTCATACCTTGTGAACAAGATGCAAGACAGAAGGTTGCAGAACTCAAGCATCTTCTGGATTCTGAAAAGAGTAGGGGAAGACTTTTAAAAGCAATTTTACAGGCTAAGTTGTCCAACCGGATACCGGAAATACATGGCCGACTGGGTGATTTAGGTGCCATTGATGAGCAATATGATGTTGCCATATCAACAGCTTGCCCTAGACTTGATAATATTGTGGTGGAAACAACTGCTGGAGCAATAGCTTGTTTTGAGCTACTCCGAAGAGAGCATCTTGGTGTTGCTCATTTCATGATAATGGAAAAGATCACTGATTGTTTACCAGTGAGGAAGGAGAATGTAAGAACTCCAGAAGGAGTTCCACGCCTTTTCGATCTAGTTAGGGTTGAGGACCAGAGAATGAAACTTGCTTTCTTTGAGGCACTGAAGAATACTGTTGTAGCTAAAGATCTAGATCAGGCAGAACGTATTGCATATGGAGGAAACCCCAAATTTCCAGTGGTAACACTTGATGGTGCAGTATTTGAAATATCTGGTACTATGAGTGGTGGTGGAAGTAAGCCATGTGGTAGTAAGATTGGCCGATCTATATGTACTACTGTAGTGTCAGAAGAAGCTATTACAGATGCCAGCAGGGGGCTTGCCAGTATGGCTGATAAATTAAACGATATCCGCCAACATATTGCTGATGCAGCAAAGAGATATCAATCCTCTGAAGATGCAATTGCACACTGGGAGTTGGAATTAGCTAAAAGCGAGAAAGAGTTTGACAGTTTGAGTTCACAACATAGTTATCTTGAAAAGCAAATTCTTTCCTTGGAGGCTGCATCACAGCCAAAGAAGGATGAGCTTGATCAGCTAAAAAAACTTGATAGTATCATTTCTGTTGAAGGAAATAAGATCGACGAACTCATCCGAGGATCTAAGCAGCTTAAGGAAAAGGCCCTGGAGCTTCAGCATAAAATAGAAAATGCTGGTGGTGAAAGATTAAAAGTACAGAAGTCAAAGGTTGATAATATTAAGTCCGATATTGATGAAAAGAACACTGAAATCAACCACCACAAAATCCAGATTGAGACTGGCCAGAAAACTATAAAGGAGTTGAGAAAAGTGATTCAAGATTCAAAGAAGAAGAAAGAACGACATGACAAGGAGAAGGAAACGTTATGTGAAAATTTCAAAGAAAAAGAAGAGAAGGCATCTCCACTGATTGATAAACATAAAGACGTGTTGGATAAAGCAAAATCTGAGTATTGCAATATGAAGAAGACTCTGTATGAATTGCGGGCATGGAAGGATAAAGCTGAGTTTGAACTAGAAGATATGAAGAAGAAATACAAGGATCTCAAATTTGAGGGGAAGGCTTACAAGGTCAGGCTTGATAAGTTGCAAATTGCCCTTATAAAGCATATGGATCAAATTCATAAAGATTTAGTGGACTCTGAAAAGGTTCAAGCAACCTTGGCAGATGAGCATCTTAGTTGTTGCGACCTTAAAAGGGCCATTGAAATGGCAGCACTATTAGAAGCACAAATGAGAGAGATGAATCCAAATCTTGATTCAATCTCAGAGTACCAAAGAAAGGTCTTATTATATAACGAAAGAGTGGAGGATCTGAGTATTGTCACAAAACAGCGTGATGACATAAAGAGGCAGTATGATGAATTGAGAAAGAAGAGGTTGGATGACTTTATGGCAGGATTTAATGCAATATCTATGAAGTTAAAGGAAATGTATCAGATGATCACGCTGGGAGGTGATGCAGAACTTGAGCTAGTCGACTCCCTGGATCCTTTCTCCGAAGGTGTTGTCTTCAGCGTGAGACCACCAAAGAAGAGTTGGAAAAATATTTCAAACTTATCTGGTGGTGAAAAGATATTGAGCTCATTGGCTCTCGTGTTTGCACTGCATCATTACAAGCCTGCGCCTCTTTTTGTCATGGATGAAATTGATGCTGCCTTAGACTTTAAGAATGTCTCCATCGTTGGTCATTACGTGAAGGACCAGACAAAGGATGCACAGTTCATAATCGTAAGCCTTAGAAATAATATGTTTGAGCTAGCGGATCGACTTGTGGGGATATACAAAACTCATAATTGCACAAAGAGCATTACCATTAACCCTGGGAGTTTTGTGATCTGCAATCAAGCTGCTCAAGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000070 GO:0000278 GO:0000280 GO:0000819 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006323 GO:0006996 GO:0007049 GO:0007059 GO:0007076 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016043 GO:0022402 GO:0022414 GO:0030261 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048285 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051276 GO:0061458 GO:0071103 GO:0071840 GO:0098813 GO:0140014 GO:1903047

KEGG Pathways

Metabolic & Signaling

ko04111 map04111

Pfam Domains

Protein Families

SMC_N SMC_hinge

Protein Analysis

1206

Amino Acids

138.19

Weight (kDa)

5.76

Isoelectric Point (pI)

37.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SMC_N	PF02463	21 - 1188	2e-69	RecF/RecN/SMC N terminal domain
AAA_15	PF13175	22 - 359	2.5e-06	AAA ATPase domain
AAA_23	PF13476	25 - 286	2.7e-09	AAA domain
SMC_hinge	PF06470	528 - 644	3.8e-20	SMC proteins Flexible Hinge Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001236)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48600 AT5G48600
fragaria_vesca	FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_4g18080 FvH4_5g30910 FvH4_5g30910 FvH4_5g30910 FvH4_6g23280
malus_domestica	MD02G1198100.v1.1
prunus_persica	Prupe.3G267300_v2.0.a1 Prupe.3G267300_v2.0.a1
pyrus_communis	pycom02g16170
rosa_chinensis	RchiOBHm_Chr5g0008421 RchiOBHm_Chr6g0300801 RchiOBHm_Chr7g0223681 RchiOBHm_Chr7g0223741 RchiOBHm_Chr7g0226461
rosa_laevigata	RLG00000001729 RLG00000011300 RLG00000031570
rosa_multiflora	Rmu_sc0001470.1_g000005 Rmu_sc0001903.1_g000024 Rmu_sc0002826.1_g000022 Rmu_sc0008947.1_g000001
rosa_roxburghii	Rroxscaffold_3G00232810 Rroxscaffold_3G00235410 Rroxscaffold_7G00167400
rosa_rugosa	Rorug06G0299200 Rorug07G0218700 Rorug07G0219100 Rorug07G0219200 Rorug07G0237300
rosa_samantha	Rh6AG412200 Rh6BG419400 Rh6CG426900 Rh6DG412200 Rh7AG362000 Rh7AG384400 Rh7AG384500 Rh7BG353400 Rh7BG353500 Rh7BG371100 Rh7CG380600 Rh7CG403700 Rh7CG403800 Rh7DG356900 Rh7DG357400 Rh7DG383200
rosa_wichuraiana	Rw7G030730 Rw7G030770 Rw7G032280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	627, 2861
AccB1I	GGYRCC	1 cut(s)	1613
AccB7I	CCANNNNNTGG	1 cut(s)	277
AccI	GTMKAC	1 cut(s)	3237
AciI	CCGC	5 cut(s)	11, 728, 2131, 2743, 3521
AclI	AACGTT	1 cut(s)	2621
AcoI	YGGCCR	4 cut(s)	27, 1594, 2038, 2539
AcuI	CTGAAG	5 cut(s)	482, 1904, 2193, 2396, 3256
AfaI	GTAC	7 cut(s)	572, 737, 920, 1996, 2053, 2454, 3032
AfiI	CCNNNNNNNGG	6 cut(s)	277, 1582, 2094, 2956, 3040, 3469
AflII	CTTAAG	1 cut(s)	2390
AflIII	ACRYGT	1 cut(s)	2685
AjiI	CACGTC	1 cut(s)	2686
AjnI	CCWGG	9 cut(s)	306, 349, 398, 471, 879, 1122, 2403, 3243, 3580
AjuI	GAANNNNNNNTTGG	4 cut(s)	99, 131, 3001, 3033
AloI	GAACNNNNNNTCC	2 cut(s)	3464, 3496
Alw21I	GWGCWC	4 cut(s)	249, 289, 1230, 3344
Alw26I	GTCTC	3 cut(s)	2528, 3274, 3439
Ama87I	CYCGRG	2 cut(s)	288, 566
AoxI	GGCC	7 cut(s)	27, 559, 1594, 2038, 2400, 2539, 2958
ApeKI	GCWGC	7 cut(s)	1298, 2150, 2282, 2386, 2972, 3413, 3608
ArsI	GACNNNNNNTTYG	2 cut(s)	3252, 3284
AseI	ATTAAT	1 cut(s)	1020
Asp700I	GAANNNNTTC	3 cut(s)	345, 1815, 2907
AspLEI	GCGC	1 cut(s)	3385
AspS9I	GGNCC	7 cut(s)	119, 152, 591, 1849, 2401, 2958, 3460
AsuHPI	GGTGA	6 cut(s)	116, 515, 1616, 2450, 3227, 3338
AsuII	TTCGAA	1 cut(s)	1404
AsuNHI	GCTAGC	1 cut(s)	3517
AvaI	CYCGRG	2 cut(s)	288, 566
AvaII	GGWCC	5 cut(s)	119, 152, 591, 1849, 3460
BalI	TGGCCA	1 cut(s)	2541
BamHI	GGATCC	1 cut(s)	3247
BanI	GGYRCC	1 cut(s)	1613
BanII	GRGCYC	2 cut(s)	249, 3344
BarI	GAAGNNNNNNTAC	4 cut(s)	1446, 1478, 2777, 2809
BauI	CACGAG	1 cut(s)	3353
BbsI	GAAGAC	4 cut(s)	1314, 1543, 2732, 3262
Bbv12I	GWGCWC	4 cut(s)	249, 289, 1230, 3344
BbvI	GCAGC	7 cut(s)	1310, 2162, 2269, 2398, 2984, 3400, 3595
BccI	CCATC	7 cut(s)	26, 323, 695, 707, 1318, 1964, 3446
BcgI	CGANNNNNNTGC	2 cut(s)	586, 620
BciT130I	CCWGG	9 cut(s)	308, 351, 400, 473, 881, 1124, 2405, 3245, 3582
BciVI	GTATCC	2 cut(s)	1147, 1572
BclI	TGATCA	2 cut(s)	2311, 3201
BcoDI	GTCTC	3 cut(s)	2528, 3274, 3439
BfaI	CTAG	8 cut(s)	372, 1388, 1659, 1835, 1910, 2774, 3233, 3518
BfmI	CTRYAG	3 cut(s)	824, 1359, 2058
BfrI	CTTAAG	1 cut(s)	2390
BfuI	GTATCC	2 cut(s)	1147, 1572
BglI	GCCNNNNNGGC	1 cut(s)	2108
BglII	AGATCT	3 cut(s)	516, 656, 1906
BisI	GCNGC	8 cut(s)	11, 1299, 2151, 2283, 2387, 2973, 3414, 3609
BlpI	GCTNAGC	1 cut(s)	216
BlsI	GCNGC	8 cut(s)	12, 1300, 2152, 2284, 2388, 2974, 3415, 3610
Bme1390I	CCNGG	9 cut(s)	308, 351, 400, 473, 881, 1124, 2405, 3245, 3582
Bme18I	GGWCC	5 cut(s)	119, 152, 591, 1849, 3460
BmeT110I	CYCGRG	2 cut(s)	288, 566
BmgBI	CACGTC	1 cut(s)	2686
BmgT120I	GGNCC	7 cut(s)	119, 152, 591, 1849, 2401, 2958, 3460
BmiI	GGNNCC	3 cut(s)	592, 1615, 3249
BmrFI	CCNGG	9 cut(s)	308, 351, 400, 473, 881, 1124, 2405, 3245, 3582
BmrI	ACTGGG	2 cut(s)	1610, 2200
BmtI	GCTAGC	1 cut(s)	3521
BmuI	ACTGGG	2 cut(s)	1610, 2200
BpiI	GAAGAC	4 cut(s)	1314, 1543, 2732, 3262
BplI	GAGNNNNNCTC	2 cut(s)	503, 535
BpmI	CTGGAG	5 cut(s)	382, 1145, 1713, 1791, 2426
Bpu1102I	GCTNAGC	1 cut(s)	216
Bpu14I	TTCGAA	1 cut(s)	1404
BpuEI	CTTGAG	7 cut(s)	420, 525, 596, 1091, 1487, 3248, 3597
BsaAI	YACGTR	1 cut(s)	3454
BsaBI	GATNNNNATC	3 cut(s)	1911, 2164, 2931
BsaI	GGTCTC	1 cut(s)	3274
BsaJI	CCNNGG	9 cut(s)	53, 1205, 2274, 2374, 2403, 2919, 3243, 3580, 3581
BsaWI	WCCGGW	2 cut(s)	1575, 1582
Bsc4I	CCNNNNNNNGG	6 cut(s)	277, 1582, 2094, 2956, 3040, 3469
Bse118I	RCCGGY	1 cut(s)	101
Bse1I	ACTGG	6 cut(s)	1605, 1781, 1955, 2103, 2195, 2542
Bse3DI	GCAATG	2 cut(s)	195, 1365
Bse8I	GATNNNNATC	3 cut(s)	1911, 2164, 2931
BseBI	CCWGG	9 cut(s)	308, 351, 400, 473, 881, 1124, 2405, 3245, 3582
BseDI	CCNNGG	9 cut(s)	53, 1205, 2274, 2374, 2403, 2919, 3243, 3580, 3581
BseJI	GATNNNNATC	3 cut(s)	1911, 2164, 2931
BseLI	CCNNNNNNNGG	6 cut(s)	277, 1582, 2094, 2956, 3040, 3469
BseMI	GCAATG	2 cut(s)	195, 1365
BseMII	CTCAG	5 cut(s)	563, 2697, 2754, 3039, 3065
BseNI	ACTGG	6 cut(s)	1605, 1781, 1955, 2103, 2195, 2542
BseRI	GAGGAG	2 cut(s)	385, 554
BseXI	GCAGC	7 cut(s)	1310, 2162, 2269, 2398, 2984, 3400, 3595
BseYI	CCCAGC	1 cut(s)	3208
BsgI	GTGCAG	2 cut(s)	1317, 1995
BshFI	GGCC	7 cut(s)	29, 561, 1596, 2040, 2402, 2541, 2960
BshNI	GGYRCC	1 cut(s)	1613
BsiHKAI	GWGCWC	4 cut(s)	249, 289, 1230, 3344
BsiHKCI	CYCGRG	2 cut(s)	288, 566
BsiSI	CCGG	4 cut(s)	14, 102, 1576, 1583
BslFI	GGGAC	4 cut(s)	346, 604, 625, 739
BslI	CCNNNNNNNGG	6 cut(s)	277, 1582, 2094, 2956, 3040, 3469
BsmAI	GTCTC	3 cut(s)	2528, 3274, 3439
BsmFI	GGGAC	4 cut(s)	346, 604, 625, 739
BsnI	GGCC	7 cut(s)	29, 561, 1596, 2040, 2402, 2541, 2960
Bso31I	GGTCTC	1 cut(s)	3274
BsoBI	CYCGRG	2 cut(s)	288, 566
Bsp119I	TTCGAA	1 cut(s)	1404
Bsp1286I	GDGCHC	4 cut(s)	249, 289, 1230, 3344
Bsp1407I	TGTACA	1 cut(s)	918
Bsp1720I	GCTNAGC	1 cut(s)	216
BspACI	CCGC	5 cut(s)	11, 728, 2131, 2743, 3521
BspANI	GGCC	7 cut(s)	29, 561, 1596, 2040, 2402, 2541, 2960
BspCNI	CTCAG	5 cut(s)	562, 2698, 2755, 3038, 3066
BspHI	TCATGA	2 cut(s)	525, 1749
BspLI	GGNNCC	3 cut(s)	592, 1615, 3249
BspMAI	CTGCAG	2 cut(s)	828, 1363
BspOI	GCTAGC	1 cut(s)	3521
BspQI	GCTCTTC	1 cut(s)	164
BspT104I	TTCGAA	1 cut(s)	1404
BspT107I	GGYRCC	1 cut(s)	1613
BspTI	CTTAAG	1 cut(s)	2390
BspTNI	GGTCTC	1 cut(s)	3274
BsrDI	GCAATG	2 cut(s)	195, 1365
BsrFI	RCCGGY	1 cut(s)	101
BsrGI	TGTACA	1 cut(s)	918
BsrI	ACTGG	6 cut(s)	1605, 1781, 1955, 2103, 2195, 2542
BssAI	RCCGGY	1 cut(s)	101
BssECI	CCNNGG	9 cut(s)	53, 1205, 2274, 2374, 2403, 2919, 3243, 3580, 3581
BssSI	CACGAG	1 cut(s)	3353
BssT1I	CCWWGG	4 cut(s)	53, 1205, 2274, 2919
Bst2BI	CACGAG	1 cut(s)	3353
Bst2UI	CCWGG	9 cut(s)	308, 351, 400, 473, 881, 1124, 2405, 3245, 3582
Bst4CI	ACNGT	7 cut(s)	419, 1186, 1421, 1894, 2059, 2231, 3480
Bst6I	CTCTTC	6 cut(s)	164, 303, 1717, 2643, 3128, 3287
BstAFI	CTTAAG	1 cut(s)	2390
BstAPI	GCANNNNNTGC	1 cut(s)	1367
BstAUI	TGTACA	1 cut(s)	918
BstBAI	YACGTR	1 cut(s)	3454
BstBI	TTCGAA	1 cut(s)	1404
BstC8I	GCNNGC	7 cut(s)	684, 1654, 2089, 2100, 2745, 3381, 3519
BstHHI	GCGC	1 cut(s)	3385
BstMAI	GTCTC	3 cut(s)	2528, 3274, 3439
BstMWI	GCNNNNNNNGC	8 cut(s)	823, 1295, 1367, 2108, 2150, 2314, 2921, 3564
BstNI	CCWGG	9 cut(s)	308, 351, 400, 473, 881, 1124, 2405, 3245, 3582
BstNSI	RCATGY	1 cut(s)	742
BstSCI	CCNGG	9 cut(s)	306, 349, 398, 471, 879, 1122, 2403, 3243, 3580
BstSFI	CTRYAG	3 cut(s)	824, 1359, 2058
BstV1I	GCAGC	7 cut(s)	1310, 2162, 2269, 2398, 2984, 3400, 3595
BstV2I	GAAGAC	4 cut(s)	1314, 1543, 2732, 3262
BstXI	CCANNNNNNTGG	2 cut(s)	787, 2968
BsuI	GTATCC	2 cut(s)	1147, 1572
BsuRI	GGCC	7 cut(s)	29, 561, 1596, 2040, 2402, 2541, 2960
BtrI	CACGTC	1 cut(s)	2686
BtsI	GCAGTG	2 cut(s)	903, 3362
BtsIMutI	CAGTG	9 cut(s)	903, 1767, 1788, 1880, 1962, 2188, 2502, 2663, 3362
Cac8I	GCNNGC	7 cut(s)	684, 1654, 2089, 2100, 2745, 3381, 3519
CciI	TCATGA	2 cut(s)	525, 1749
CfoI	GCGC	1 cut(s)	3385
Cfr10I	RCCGGY	1 cut(s)	101
Cfr13I	GGNCC	7 cut(s)	119, 152, 591, 1849, 2401, 2958, 3460
CseI	GACGC	2 cut(s)	875, 1319
Csp6I	GTAC	7 cut(s)	571, 736, 919, 1995, 2052, 2453, 3031
CviAII	CATG	9 cut(s)	25, 526, 739, 1592, 1750, 2022, 2603, 2748, 3397
CviQI	GTAC	7 cut(s)	571, 736, 919, 1995, 2052, 2453, 3031
DraI	TTTAAA	1 cut(s)	1545
EaeI	YGGCCR	4 cut(s)	27, 1594, 2038, 2539
Eam1104I	CTCTTC	6 cut(s)	164, 303, 1717, 2643, 3128, 3287
EarI	CTCTTC	6 cut(s)	164, 303, 1717, 2643, 3128, 3287
EciI	GGCGGA	1 cut(s)	2120
Ecl136II	GAGCTC	2 cut(s)	247, 3342
Eco130I	CCWWGG	4 cut(s)	53, 1205, 2274, 2919
Eco147I	AGGCCT	1 cut(s)	561
Eco24I	GRGCYC	2 cut(s)	249, 3344
Eco31I	GGTCTC	1 cut(s)	3274
Eco32I	GATATC	2 cut(s)	2128, 2162
Eco47I	GGWCC	5 cut(s)	119, 152, 591, 1849, 3460
Eco53kI	GAGCTC	2 cut(s)	247, 3342
Eco57I	CTGAAG	5 cut(s)	482, 1904, 2193, 2396, 3256
Eco88I	CYCGRG	2 cut(s)	288, 566
EcoICRI	GAGCTC	2 cut(s)	247, 3342
EcoO109I	RGGNCCY	1 cut(s)	2401
EcoRI	GAATTC	1 cut(s)	1141
EcoRII	CCWGG	9 cut(s)	306, 349, 398, 471, 879, 1122, 2403, 3243, 3580
EcoRV	GATATC	2 cut(s)	2128, 2162
EcoT14I	CCWWGG	4 cut(s)	53, 1205, 2274, 2919
EcoT38I	GRGCYC	2 cut(s)	249, 3344
ErhI	CCWWGG	4 cut(s)	53, 1205, 2274, 2919
FaeI	CATG	9 cut(s)	28, 529, 742, 1595, 1753, 2025, 2606, 2751, 3400
FaqI	GGGAC	4 cut(s)	346, 604, 625, 739
FatI	CATG	9 cut(s)	24, 525, 738, 1591, 1749, 2021, 2602, 2747, 3396
FauI	CCCGC	1 cut(s)	2736
FauNDI	CATATG	3 cut(s)	930, 1933, 2868
FbaI	TGATCA	2 cut(s)	2311, 3201
FblI	GTMKAC	1 cut(s)	3237
Fnu4HI	GCNGC	8 cut(s)	11, 1299, 2151, 2283, 2387, 2973, 3414, 3609
FriOI	GRGCYC	2 cut(s)	249, 3344
Fsp4HI	GCNGC	8 cut(s)	11, 1299, 2151, 2283, 2387, 2973, 3414, 3609
FspBI	CTAG	8 cut(s)	372, 1388, 1659, 1835, 1910, 2774, 3233, 3518
GlaI	GCGC	1 cut(s)	3384
GluI	GCNGC	8 cut(s)	11, 1299, 2151, 2283, 2387, 2973, 3414, 3609
GsaI	CCCAGC	1 cut(s)	3212
GsuI	CTGGAG	5 cut(s)	382, 1145, 1713, 1791, 2426
HaeIII	GGCC	7 cut(s)	29, 561, 1596, 2040, 2402, 2541, 2960
HapII	CCGG	4 cut(s)	14, 102, 1576, 1583
HgaI	GACGC	2 cut(s)	875, 1319
HhaI	GCGC	1 cut(s)	3385
Hin1II	CATG	9 cut(s)	28, 529, 742, 1595, 1753, 2025, 2606, 2751, 3400
Hin6I	GCGC	1 cut(s)	3383
HinP1I	GCGC	1 cut(s)	3383
HincII	GTYRAC	2 cut(s)	469, 3238
HindII	GTYRAC	2 cut(s)	469, 3238
HindIII	AAGCTT	1 cut(s)	1265
HpaII	CCGG	4 cut(s)	14, 102, 1576, 1583
HphI	GGTGA	6 cut(s)	116, 515, 1616, 2450, 3227, 3338
Hpy166II	GTNNAC	8 cut(s)	107, 469, 919, 1472, 1778, 2240, 2896, 3238
Hpy8I	GTNNAC	8 cut(s)	107, 469, 919, 1472, 1778, 2240, 2896, 3238
Hpy99I	CGWCG	1 cut(s)	2366
HpyCH4III	ACNGT	7 cut(s)	419, 1186, 1421, 1894, 2059, 2231, 3480
HpyCH4IV	ACGT	6 cut(s)	177, 335, 1924, 2621, 2685, 3453
HpyF10VI	GCNNNNNNNGC	8 cut(s)	823, 1295, 1367, 2108, 2150, 2314, 2921, 3564
HpySE526I	ACGT	6 cut(s)	177, 335, 1924, 2621, 2685, 3453
Hsp92II	CATG	9 cut(s)	28, 529, 742, 1595, 1753, 2025, 2606, 2751, 3400
HspAI	GCGC	1 cut(s)	3383
Ksp22I	TGATCA	2 cut(s)	2311, 3201
LguI	GCTCTTC	1 cut(s)	164
LmnI	GCTCC	6 cut(s)	244, 883, 904, 1000, 1694, 2407
Lsp1109I	GCAGC	7 cut(s)	1310, 2162, 2269, 2398, 2984, 3400, 3595
MaeI	CTAG	8 cut(s)	372, 1388, 1659, 1835, 1910, 2774, 3233, 3518
MaeII	ACGT	6 cut(s)	177, 335, 1924, 2621, 2685, 3453
MaeIII	GTNAC	3 cut(s)	439, 1960, 3082
MfeI	CAATTG	2 cut(s)	785, 2181
MhlI	GDGCHC	4 cut(s)	249, 289, 1230, 3344
MlsI	TGGCCA	1 cut(s)	2541
MluNI	TGGCCA	1 cut(s)	2541
MlyI	GAGTC	5 cut(s)	50, 647, 2722, 2891, 3233
MmeI	TCCRAC	7 cut(s)	130, 1086, 1596, 2178, 2670, 3120, 3278
Mox20I	TGGCCA	1 cut(s)	2541
MroXI	GAANNNNTTC	3 cut(s)	345, 1815, 2907
MscI	TGGCCA	1 cut(s)	2541
MslI	CAYNNNNRTG	3 cut(s)	1734, 1754, 2966
Msp20I	TGGCCA	1 cut(s)	2541
MspCI	CTTAAG	1 cut(s)	2390
MspI	CCGG	4 cut(s)	14, 102, 1576, 1583
MspR9I	CCNGG	9 cut(s)	308, 351, 400, 473, 881, 1124, 2405, 3245, 3582
MunI	CAATTG	2 cut(s)	785, 2181
MvaI	CCWGG	9 cut(s)	308, 351, 400, 473, 881, 1124, 2405, 3245, 3582
MwoI	GCNNNNNNNGC	8 cut(s)	823, 1295, 1367, 2108, 2150, 2314, 2921, 3564
NdeI	CATATG	3 cut(s)	930, 1933, 2868
NheI	GCTAGC	1 cut(s)	3517
NlaIII	CATG	9 cut(s)	28, 529, 742, 1595, 1753, 2025, 2606, 2751, 3400
NlaIV	GGNNCC	3 cut(s)	592, 1615, 3249
NmuCI	GTSAC	1 cut(s)	3082
NspI	RCATGY	1 cut(s)	742
NspV	TTCGAA	1 cut(s)	1404
PaeR7I	CTCGAG	2 cut(s)	288, 566
PagI	TCATGA	2 cut(s)	525, 1749
PasI	CCCWGGG	1 cut(s)	3581
PceI	AGGCCT	1 cut(s)	561
PciSI	GCTCTTC	1 cut(s)	164
PdmI	GAANNNNTTC	3 cut(s)	345, 1815, 2907
PflMI	CCANNNNNTGG	1 cut(s)	277
PfoI	TCCNGGA	4 cut(s)	306, 349, 879, 1122
PkrI	GCNGC	8 cut(s)	12, 1300, 2152, 2284, 2388, 2974, 3415, 3610
PleI	GAGTC	5 cut(s)	49, 646, 2722, 2891, 3233
PpsI	GAGTC	5 cut(s)	49, 646, 2722, 2891, 3233
Ppu21I	YACGTR	1 cut(s)	3454
PshBI	ATTAAT	1 cut(s)	1020
PsiI	TTATAA	2 cut(s)	627, 2861
Psp124BI	GAGCTC	2 cut(s)	249, 3344
Psp1406I	AACGTT	1 cut(s)	2621
Psp6I	CCWGG	9 cut(s)	306, 349, 398, 471, 879, 1122, 2403, 3243, 3580
PspFI	CCCAGC	1 cut(s)	3208
PspGI	CCWGG	9 cut(s)	306, 349, 398, 471, 879, 1122, 2403, 3243, 3580
PspN4I	GGNNCC	3 cut(s)	592, 1615, 3249
PspPI	GGNCC	7 cut(s)	119, 152, 591, 1849, 2401, 2958, 3460
PspXI	VCTCGAGB	2 cut(s)	288, 566
PstI	CTGCAG	2 cut(s)	828, 1363
RsaI	GTAC	7 cut(s)	572, 737, 920, 1996, 2053, 2454, 3032
RsaNI	GTAC	7 cut(s)	571, 736, 919, 1995, 2052, 2453, 3031
RseI	CAYNNNNRTG	3 cut(s)	1734, 1754, 2966
SacI	GAGCTC	2 cut(s)	249, 3344
SalI	GTCGAC	1 cut(s)	3236
SapI	GCTCTTC	1 cut(s)	164
SatI	GCNGC	8 cut(s)	11, 1299, 2151, 2283, 2387, 2973, 3414, 3609
Sau96I	GGNCC	7 cut(s)	119, 152, 591, 1849, 2401, 2958, 3460
SchI	GAGTC	5 cut(s)	50, 647, 2722, 2891, 3233
ScrFI	CCNGG	9 cut(s)	308, 351, 400, 473, 881, 1124, 2405, 3245, 3582
SduI	GDGCHC	4 cut(s)	249, 289, 1230, 3344
SfcI	CTRYAG	3 cut(s)	824, 1359, 2058
Sfr274I	CTCGAG	2 cut(s)	288, 566
SfuI	TTCGAA	1 cut(s)	1404
SgrAI	CRCCGGYG	1 cut(s)	101
SinI	GGWCC	5 cut(s)	119, 152, 591, 1849, 3460
SlaI	CTCGAG	2 cut(s)	288, 566
SmiMI	CAYNNNNRTG	3 cut(s)	1734, 1754, 2966
SseBI	AGGCCT	1 cut(s)	561
SsiI	CCGC	5 cut(s)	11, 728, 2131, 2743, 3521
SspI	AATATT	3 cut(s)	1672, 2476, 3307
SspMI	CTAG	8 cut(s)	372, 1388, 1659, 1835, 1910, 2774, 3233, 3518
SstI	GAGCTC	2 cut(s)	249, 3344
StuI	AGGCCT	1 cut(s)	561
StyD4I	CCNGG	9 cut(s)	306, 349, 398, 471, 879, 1122, 2403, 3243, 3580
StyI	CCWWGG	4 cut(s)	53, 1205, 2274, 2919
TaaI	ACNGT	7 cut(s)	419, 1186, 1421, 1894, 2059, 2231, 3480
TaiI	ACGT	6 cut(s)	180, 338, 1927, 2624, 2688, 3456
TatI	WGTACW	3 cut(s)	918, 2051, 2452
TauI	GCSGC	1 cut(s)	13
TscAI	CASTG	9 cut(s)	903, 1774, 1788, 1887, 1962, 2195, 2509, 2670, 3369
TseFI	GTSAC	1 cut(s)	3082
TseI	GCWGC	7 cut(s)	1298, 2150, 2282, 2386, 2972, 3413, 3608
Tsp45I	GTSAC	1 cut(s)	3082
TspGWI	ACGGA	1 cut(s)	1427
TspRI	CASTG	9 cut(s)	903, 1774, 1788, 1887, 1962, 2195, 2509, 2670, 3369
Van91I	CCANNNNNTGG	1 cut(s)	277
Vha464I	CTTAAG	1 cut(s)	2390
VpaK11BI	GGWCC	5 cut(s)	119, 152, 591, 1849, 3460
VspI	ATTAAT	1 cut(s)	1020
XbaI	TCTAGA	1 cut(s)	1909
XceI	RCATGY	1 cut(s)	742
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1954, 2534, 3294
XhoI	CTCGAG	2 cut(s)	288, 566
XmiI	GTMKAC	1 cut(s)	3237
XmnI	GAANNNNTTC	3 cut(s)	345, 1815, 2907
XspI	CTAG	8 cut(s)	372, 1388, 1659, 1835, 1910, 2774, 3233, 3518

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.