Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0001236

Physical Location & Seq

Genomic Coordinates Forward (+)

18519734 .. 18528833

Gene Structure 9100 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00232810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3279 bp

ATGGAATCGCAGCCGGACAAGTTCATGGCCGATGGCTTGGAGTCGAGGAAGCCGAGGCTGTTCATCAGGGAAATGGTTATGAGCAACTTCAAGTCTTACGCCGGTGAACAACGCGTTGGTCCTTTCCACAAGAGTTTTTCTGCTGTTGTTGGACCTAATGGGAGTGGGAAGAGCAACGTCATAGATGCAAAGCTCTTTGTATTCGGAAAACGAGCTAAGCAGATGCGACTTAACAGAGTTTCGGAGCTCATTCATAACTGCACTAATTGCCACAATATGGATAGTGCTCGAGTTTCTCTTCATTTCCAGGAGATCATTGATTTGGACGATGGGACGTTTGAAGCTGTTCCAGGAAGTGATTTTGTAATCACTAGGGTAGCGTATCGGGACAACTCCTCCAGGTATTACATCAATGACCGTTCAAGCAATTTTACTCAAGTTACCAAGAAACTGAAAGGGAAAGGAGTTGACCTGGACAACAATCGCTTCTTGATTCTTCAGGGTGATGTTGAGCAGATCTCACTCATGAAGCCAAAAGCTCAAGGATCTCAGGATGAAGGCCTCCTCGAGTACCTTAAGGACATTATTGGGACCAGCAAATATGTCGAAAAAATTGACGAATCTTATAAAGAGATAGAGTTCCTAAATGCTAAAAGATCTGGTGTCATGCAAATGGTTAAGCTGGCAGAGAAAGAAAGAGATGGCTTGGAGGATGGGAAGAATGAAGCCAAAGCTTACATGCTGAAAGAGTTGTCCCTTCTAAATTGGCAAGAGAAAGCCACACAATTGGCTTATGAAGATACCTCTGCAAAAATGCTTGATCTGCAGGAAACTATAAAAAGCTTAGAAGAAAATCTGAACAATGATAGATATAAGGATGAATTTAAATATCTTGAAAAGGAGCGTGCAAAATATCTGGTGAATCTGCAATATGTGGAGCAGAAGATCAAGAAACTTGATGAAAAACTTGAAAAGGATTCATTAAAGATTAATGACAGAGAGAGAGAATGTGAGAACTCGACTAATCAGATCCCAGAACTTGAGAAAAGTATTCCCCAACTGCGAAAACTCTTGTTGGATGAAGAAAAAGTCCTGGAGGAAATTATTGAGAATTGCAAAGGGAATACCGAAACATATCGCTGTGAGCTTACAACAGTTCGTTCCAGATTAGAACCTTGGGAAAAACAGCTGATTGAGCACCGGAAGAAATTTGATGTTGCATATACTGAACACAAGCTTTTGGATGAAAAGCATCAAGCTGGTCGTGCAGCTTTTGAAGACGCTCGAAAGCAGATGGATGATATATTGGGAATGATACATACAAAAACTGCAGACATTGCAAAAATTCGGTGTGAGCTAGAAAAGAGCAAGTTCAAAAGGACGGAATACCGTAAAGCAGAAAAGAACAAGAAGCTCTCATACCTTGTGAACAAGCTGCAAGACAGAAGGTTGCAGAACTCAAGCATCTTCTGGATTCCGAAAAGATCGAGTAGGGGAAGACTTTTAAAAGCAATTTTACAGGCTAAGTTGTCCAACCAGATACCAGGAATACATGGCCGACTGGGTGATTTAGGTGCCATTGATGAGCAATATGATGTTGCCATATCAACAGCTTGCCCTGGACTTGATTATATTGTGGTGGAAACAACTGGTGGAGCAATAGCTTGTGTTGAGCTACTCCGAAGAGAGCATCTTGGTGTTGCTCATTTCATGATAATGGAAAAGATCACTGATTGTTTACCAGTGAGGAAGGAGAATGTAAGAACTCCAGAAGGAGTTCCAGAGAATGAGGACCAGAGAATGAAACTTGCTTTCTTTGAGGCACTGAAGAATACTGTTGTAGCTAAAGATCTAGATCAGGCAGAACGTATTGCATATGGAGGAAACCACAAATTTCGAGTGGTAACACTTGATGGTGTAGTATTTGAAATATCTGGTACTATGAGTGGTGGTGGAAGTAAGCCATGTGGTAGTAAGATTGGCCGATCTATATGTACTACTGTAGTGTCAGAAGAAGCTATTACAGATGCCAGCAGGGGGCTTGCCAGTATGGCTGATAAATTAAACGATATCCGCCAACATATTGCTGATGCAGCAAAGAGATATCAAGCCTCTGAAGATGCAATTGCACACTGGGAGTTGGAATTAGCTAAAAGCGAGAAAGAGGATATTGATGAAAAGAACACTGAAATCAACCACCACAAAGTCCAGATTGAGACTGGCCAGAAAACTATAAAGGAGTTGAGAAAAGTGATTCAAGATTCAAAGAAGAAGAAAGAACGACATGACAAGGAGAAGGAAACGTTACGTGAAAATTTCAAAGAAAAAGAAGAGAAGGCATCTCTAGTTCAAGAGAACTTCGAGGAAACACAGAAGATAAGTCAAAATCTGACATATCGTATTTCTGGTCTTGTGCTGCTGATTGATAAACATAAAGAAGTGTTGGATAAAGCAAAATCTGAGTATTGCAATATGAAAAAGACTCTGTATGAATTGCGGGCATGGAAGGATAAAGCTGAGTTTGAACTAGAAGATATGAAGAAGAAATACAAGGATCTGAAATTTGAGGGGAAGGCTTACAAGGCCAGGCTTGATAAGTTGCAAATCGCCCTCATAAAGCATATGGATCAGGCCATTGAAATGGCAGCACTACTAGAAGCACAAATGAGAGAGATGAATCCAAATCTTGATTCAATCTCAGAGTACCAAAGAAAGGTCTCATTATATAACGAAAGAGTGGAGGATCTGAGTATTGTCACAAAACAGCGTGATGACATAAAGAGGCAGTCTGATGAATTGAGAAAGAAGAGGCATGTCACCTTTTTGGGCCGGGTTGCTTTTGCTACTTTCCCTCTGATGATCACGCTGGGAGGTGATGCAGAACTTGAGCTAGTCGACTCCCTGGATCCTTTCTCCGAAGGTGTTGTCTTCAGCGTGAGACCACCAAAGAAGAGTTGGAAAAATATTTCAAACTTATCTGGTGGTGAAAAGACATTGAGCTCATTGGCTCTCGTGTTTGCACTGCATCATTACAAGCCTACGCCTCTTTATGTCATGGATGAAATTGATGCTGCTTTAGACTTTAAGAATGTCTCCATCGTTGGTCATTACGTGAAGGACCAGACAAAGGATGCACAGTTCATAATCGTAAGCCTTAGAAATAATATGTTTGAGCTAGCGGATCGAGTTGTGGGGATATACAAAACTCATAATTGCACAAAGAGCATTACCATTAACCCAGGGAGTTTTGTGATCTGCAATCAAGCTGCTCAAGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000070 GO:0000278 GO:0000280 GO:0000819 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006323 GO:0006996 GO:0007049 GO:0007059 GO:0007076 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016043 GO:0022402 GO:0022414 GO:0030261 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048285 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051276 GO:0061458 GO:0071103 GO:0071840 GO:0098813 GO:0140014 GO:1903047

KEGG Pathways

Metabolic & Signaling

ko04111 map04111

Pfam Domains

Protein Families

SMC_N SMC_hinge

Protein Analysis

1092

Amino Acids

125.31

Weight (kDa)

8.08

Isoelectric Point (pI)

35.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SMC_N	PF02463	21 - 1074	9.6e-70	RecF/RecN/SMC N terminal domain
SMC_hinge	PF06470	515 - 625	1.9e-12	SMC proteins Flexible Hinge Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001236)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48600 AT5G48600
fragaria_vesca	FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_4g18080 FvH4_5g30910 FvH4_5g30910 FvH4_5g30910 FvH4_6g23280
malus_domestica	MD02G1198100.v1.1
prunus_persica	Prupe.3G267300_v2.0.a1 Prupe.3G267300_v2.0.a1
pyrus_communis	pycom02g16170
rosa_chinensis	RchiOBHm_Chr5g0008421 RchiOBHm_Chr6g0300801 RchiOBHm_Chr7g0223681 RchiOBHm_Chr7g0223741 RchiOBHm_Chr7g0226461
rosa_laevigata	RLG00000001729 RLG00000011300 RLG00000031570
rosa_multiflora	Rmu_sc0001470.1_g000005 Rmu_sc0001903.1_g000024 Rmu_sc0002826.1_g000022 Rmu_sc0008947.1_g000001
rosa_roxburghii	Rroxscaffold_3G00232810 Rroxscaffold_3G00235410 Rroxscaffold_7G00167400
rosa_rugosa	Rorug06G0299200 Rorug07G0218700 Rorug07G0219100 Rorug07G0219200 Rorug07G0237300
rosa_samantha	Rh6AG412200 Rh6BG419400 Rh6CG426900 Rh6DG412200 Rh7AG362000 Rh7AG384400 Rh7AG384500 Rh7BG353400 Rh7BG353500 Rh7BG371100 Rh7CG380600 Rh7CG403700 Rh7CG403800 Rh7DG356900 Rh7DG357400 Rh7DG383200
rosa_wichuraiana	Rw7G030730 Rw7G030770 Rw7G032280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	627
AccB1I	GGYRCC	1 cut(s)	1574
AccB7I	CCANNNNNTGG	1 cut(s)	277
AccI	GTMKAC	1 cut(s)	2895
AccII	CGCG	1 cut(s)	114
AciI	CCGC	3 cut(s)	2074, 2497, 3179
AclI	AACGTT	1 cut(s)	2303
AclWI	GGATC	8 cut(s)	553, 1024, 2562, 2634, 2751, 2900, 2913, 3189
AcoI	YGGCCR	4 cut(s)	27, 1555, 1981, 2221
AcsI	RAATTY	6 cut(s)	881, 1208, 1344, 1892, 2314, 2561
AcuI	CTGAAG	4 cut(s)	482, 1847, 2136, 2914
AfaI	GTAC	4 cut(s)	572, 1939, 1996, 2705
AfiI	CCNNNNNNNGG	4 cut(s)	277, 2037, 2713, 3127
AflII	CTTAAG	1 cut(s)	575
AflIII	ACRYGT	1 cut(s)	112
AjuI	GAANNNNNNNTTGG	4 cut(s)	99, 131, 2674, 2706
AloI	GAACNNNNNNTCC	2 cut(s)	3122, 3154
Alw21I	GWGCWC	4 cut(s)	249, 289, 1200, 3002
Alw26I	GTCTC	4 cut(s)	2210, 2722, 2932, 3097
AlwI	GGATC	8 cut(s)	553, 1024, 2562, 2634, 2751, 2900, 2913, 3189
Ama87I	CYCGRG	2 cut(s)	288, 566
AoxI	GGCC	8 cut(s)	27, 559, 1555, 1981, 2221, 2583, 2631, 2827
ApeKI	GCWGC	8 cut(s)	10, 1268, 1435, 2093, 2416, 2645, 3071, 3266
ApoI	RAATTY	6 cut(s)	881, 1208, 1344, 1892, 2314, 2561
ArsI	GACNNNNNNTTYG	2 cut(s)	2910, 2942
AseI	ATTAAT	1 cut(s)	990
Asp700I	GAANNNNTTC	2 cut(s)	345, 1776
AspS9I	GGNCC	6 cut(s)	119, 152, 591, 1792, 2827, 3118
AsuC2I	CCSGG	1 cut(s)	2831
AsuHPI	GGTGA	7 cut(s)	116, 515, 931, 1577, 2809, 2885, 2996
AsuNHI	GCTAGC	1 cut(s)	3175
AvaI	CYCGRG	2 cut(s)	288, 566
AvaII	GGWCC	5 cut(s)	119, 152, 591, 1792, 3118
BalI	TGGCCA	1 cut(s)	2223
BamHI	GGATCC	1 cut(s)	2905
BanI	GGYRCC	1 cut(s)	1574
BanII	GRGCYC	2 cut(s)	249, 3002
BarI	GAAGNNNNNNTAC	4 cut(s)	1403, 1435, 2531, 2563
BauI	CACGAG	1 cut(s)	3011
BbsI	GAAGAC	3 cut(s)	1284, 1504, 2920
Bbv12I	GWGCWC	4 cut(s)	249, 289, 1200, 3002
BbvI	GCAGC	8 cut(s)	22, 1280, 1422, 2105, 2403, 2657, 3058, 3253
BccI	CCATC	7 cut(s)	26, 323, 695, 707, 1288, 1907, 3104
BcgI	CGANNNNNNTGC	2 cut(s)	586, 620
BclI	TGATCA	1 cut(s)	2859
BcnI	CCSGG	1 cut(s)	2831
BcoDI	GTCTC	4 cut(s)	2210, 2722, 2932, 3097
BfaI	CTAG	8 cut(s)	372, 1358, 1853, 2345, 2528, 2654, 2891, 3176
BfmI	CTRYAG	3 cut(s)	824, 1329, 2001
BfrI	CTTAAG	1 cut(s)	575
BglI	GCCNNNNNGGC	1 cut(s)	2051
BglII	AGATCT	3 cut(s)	516, 656, 1849
BisI	GCNGC	8 cut(s)	11, 1269, 1436, 2094, 2417, 2646, 3072, 3267
BlpI	GCTNAGC	1 cut(s)	216
BlsI	GCNGC	8 cut(s)	12, 1270, 1437, 2095, 2418, 2647, 3073, 3268
Bme18I	GGWCC	5 cut(s)	119, 152, 591, 1792, 3118
BmeT110I	CYCGRG	2 cut(s)	288, 566
BmgT120I	GGNCC	6 cut(s)	119, 152, 591, 1792, 2827, 3118
BmiI	GGNNCC	3 cut(s)	592, 1576, 2907
BmrI	ACTGGG	2 cut(s)	1571, 2143
BmtI	GCTAGC	1 cut(s)	3179
BmuI	ACTGGG	2 cut(s)	1571, 2143
BpiI	GAAGAC	3 cut(s)	1284, 1504, 2920
BplI	GAGNNNNNCTC	2 cut(s)	503, 535
BpmI	CTGGAG	3 cut(s)	382, 1115, 1752
Bpu1102I	GCTNAGC	1 cut(s)	216
BpuEI	CTTGAG	6 cut(s)	420, 525, 1061, 1444, 2906, 3255
BpuMI	CCSGG	1 cut(s)	2831
BsaAI	YACGTR	2 cut(s)	2309, 3112
BsaBI	GATNNNNATC	1 cut(s)	1854
BsaI	GGTCTC	2 cut(s)	2722, 2932
BsaJI	CCNNGG	6 cut(s)	53, 1175, 1618, 2901, 3238, 3239
BsaWI	WCCGGW	1 cut(s)	1200
BsaXI	ACNNNNNCTCC	2 cut(s)	380, 410
Bsc4I	CCNNNNNNNGG	4 cut(s)	277, 2037, 2713, 3127
Bse118I	RCCGGY	1 cut(s)	101
Bse1I	ACTGG	6 cut(s)	1566, 1654, 1742, 2046, 2138, 2224
Bse3DI	GCAATG	1 cut(s)	1335
Bse8I	GATNNNNATC	1 cut(s)	1854
BseDI	CCNNGG	6 cut(s)	53, 1175, 1618, 2901, 3238, 3239
BseGI	GGATG	8 cut(s)	559, 718, 883, 1084, 1249, 1303, 3064, 3136
BseJI	GATNNNNATC	1 cut(s)	1854
BseLI	CCNNNNNNNGG	4 cut(s)	277, 2037, 2713, 3127
BseMI	GCAATG	1 cut(s)	1335
BseMII	CTCAG	5 cut(s)	563, 2451, 2508, 2712, 2738
BseNI	ACTGG	6 cut(s)	1566, 1654, 1742, 2046, 2138, 2224
BseRI	GAGGAG	2 cut(s)	385, 554
BseXI	GCAGC	8 cut(s)	22, 1280, 1422, 2105, 2403, 2657, 3058, 3253
BseYI	CCCAGC	1 cut(s)	2866
BsgI	GTGCAG	2 cut(s)	244, 1287
Bsh1236I	CGCG	1 cut(s)	114
BshFI	GGCC	8 cut(s)	29, 561, 1557, 1983, 2223, 2585, 2633, 2829
BshNI	GGYRCC	1 cut(s)	1574
BsiHKAI	GWGCWC	4 cut(s)	249, 289, 1200, 3002
BsiHKCI	CYCGRG	2 cut(s)	288, 566
BsiSI	CCGG	4 cut(s)	14, 102, 1201, 2830
BslFI	GGGAC	4 cut(s)	346, 401, 604, 739
BslI	CCNNNNNNNGG	4 cut(s)	277, 2037, 2713, 3127
BsmAI	GTCTC	4 cut(s)	2210, 2722, 2932, 3097
BsmFI	GGGAC	4 cut(s)	346, 401, 604, 739
BsnI	GGCC	8 cut(s)	29, 561, 1557, 1983, 2223, 2585, 2633, 2829
Bso31I	GGTCTC	2 cut(s)	2722, 2932
BsoBI	CYCGRG	2 cut(s)	288, 566
Bsp1286I	GDGCHC	4 cut(s)	249, 289, 1200, 3002
Bsp1720I	GCTNAGC	1 cut(s)	216
BspACI	CCGC	3 cut(s)	2074, 2497, 3179
BspANI	GGCC	8 cut(s)	29, 561, 1557, 1983, 2223, 2585, 2633, 2829
BspCNI	CTCAG	5 cut(s)	562, 2452, 2509, 2711, 2739
BspFNI	CGCG	1 cut(s)	114
BspHI	TCATGA	2 cut(s)	525, 1710
BspLI	GGNNCC	3 cut(s)	592, 1576, 2907
BspMAI	CTGCAG	2 cut(s)	828, 1333
BspOI	GCTAGC	1 cut(s)	3179
BspPI	GGATC	8 cut(s)	553, 1024, 2562, 2634, 2751, 2900, 2913, 3189
BspQI	GCTCTTC	1 cut(s)	164
BspT107I	GGYRCC	1 cut(s)	1574
BspTI	CTTAAG	1 cut(s)	575
BspTNI	GGTCTC	2 cut(s)	2722, 2932
BsrDI	GCAATG	1 cut(s)	1335
BsrFI	RCCGGY	1 cut(s)	101
BsrI	ACTGG	6 cut(s)	1566, 1654, 1742, 2046, 2138, 2224
BssAI	RCCGGY	1 cut(s)	101
BssECI	CCNNGG	6 cut(s)	53, 1175, 1618, 2901, 3238, 3239
BssSI	CACGAG	1 cut(s)	3011
BssT1I	CCWWGG	1 cut(s)	1175
Bst2BI	CACGAG	1 cut(s)	3011
Bst4CI	ACNGT	6 cut(s)	419, 1156, 1391, 1837, 2002, 3138
Bst6I	CTCTTC	6 cut(s)	164, 303, 1678, 2325, 2801, 2945
BstAFI	CTTAAG	1 cut(s)	575
BstAPI	GCANNNNNTGC	2 cut(s)	267, 1337
BstBAI	YACGTR	2 cut(s)	2309, 3112
BstC8I	GCNNGC	7 cut(s)	684, 906, 1615, 2032, 2043, 2499, 3177
BstDEI	CTNAG	9 cut(s)	216, 549, 844, 1524, 2460, 2517, 2698, 2747, 3155
BstF5I	GGATG	8 cut(s)	559, 718, 883, 1084, 1249, 1303, 3064, 3136
BstFNI	CGCG	1 cut(s)	114
BstMAI	GTCTC	4 cut(s)	2210, 2722, 2932, 3097
BstMWI	GCNNNNNNNGC	9 cut(s)	267, 823, 1195, 1265, 1337, 2051, 2093, 2582, 3222
BstNSI	RCATGY	2 cut(s)	742, 2816
BstSFI	CTRYAG	3 cut(s)	824, 1329, 2001
BstUI	CGCG	1 cut(s)	114
BstV1I	GCAGC	8 cut(s)	22, 1280, 1422, 2105, 2403, 2657, 3058, 3253
BstV2I	GAAGAC	3 cut(s)	1284, 1504, 2920
BstX2I	RGATCY	8 cut(s)	516, 545, 656, 1029, 1849, 2554, 2743, 2905
BstXI	CCANNNNNNTGG	2 cut(s)	787, 2641
BstYI	RGATCY	8 cut(s)	516, 545, 656, 1029, 1849, 2554, 2743, 2905
BsuRI	GGCC	8 cut(s)	29, 561, 1557, 1983, 2223, 2585, 2633, 2829
BtsCI	GGATG	8 cut(s)	559, 718, 883, 1084, 1249, 1303, 3064, 3136
BtsI	GCAGTG	1 cut(s)	3020
BtsIMutI	CAGTG	6 cut(s)	1728, 1749, 1823, 2131, 2184, 3020
Cac8I	GCNNGC	7 cut(s)	684, 906, 1615, 2032, 2043, 2499, 3177
CciI	TCATGA	2 cut(s)	525, 1710
Cfr10I	RCCGGY	1 cut(s)	101
Cfr13I	GGNCC	6 cut(s)	119, 152, 591, 1792, 2827, 3118
CseI	GACGC	1 cut(s)	1289
Csp6I	GTAC	4 cut(s)	571, 1938, 1995, 2704
CviQI	GTAC	4 cut(s)	571, 1938, 1995, 2704
DdeI	CTNAG	9 cut(s)	216, 549, 844, 1524, 2460, 2517, 2698, 2747, 3155
DraI	TTTAAA	2 cut(s)	886, 1506
EaeI	YGGCCR	4 cut(s)	27, 1555, 1981, 2221
Eam1104I	CTCTTC	6 cut(s)	164, 303, 1678, 2325, 2801, 2945
EarI	CTCTTC	6 cut(s)	164, 303, 1678, 2325, 2801, 2945
EciI	GGCGGA	1 cut(s)	2063
Ecl136II	GAGCTC	2 cut(s)	247, 3000
Eco130I	CCWWGG	1 cut(s)	1175
Eco147I	AGGCCT	1 cut(s)	561
Eco24I	GRGCYC	2 cut(s)	249, 3002
Eco31I	GGTCTC	2 cut(s)	2722, 2932
Eco32I	GATATC	2 cut(s)	2071, 2105
Eco47I	GGWCC	5 cut(s)	119, 152, 591, 1792, 3118
Eco53kI	GAGCTC	2 cut(s)	247, 3000
Eco57I	CTGAAG	4 cut(s)	482, 1847, 2136, 2914
Eco88I	CYCGRG	2 cut(s)	288, 566
EcoICRI	GAGCTC	2 cut(s)	247, 3000
EcoRV	GATATC	2 cut(s)	2071, 2105
EcoT14I	CCWWGG	1 cut(s)	1175
EcoT38I	GRGCYC	2 cut(s)	249, 3002
ErhI	CCWWGG	1 cut(s)	1175
FaqI	GGGAC	4 cut(s)	346, 401, 604, 739
FauI	CCCGC	1 cut(s)	2490
FauNDI	CATATG	2 cut(s)	1876, 2622
FbaI	TGATCA	1 cut(s)	2859
FblI	GTMKAC	1 cut(s)	2895
Fnu4HI	GCNGC	8 cut(s)	11, 1269, 1436, 2094, 2417, 2646, 3072, 3267
FokI	GGATG	8 cut(s)	566, 725, 890, 1091, 1256, 1310, 3071, 3143
FriOI	GRGCYC	2 cut(s)	249, 3002
Fsp4HI	GCNGC	8 cut(s)	11, 1269, 1436, 2094, 2417, 2646, 3072, 3267
FspBI	CTAG	8 cut(s)	372, 1358, 1853, 2345, 2528, 2654, 2891, 3176
GluI	GCNGC	8 cut(s)	11, 1269, 1436, 2094, 2417, 2646, 3072, 3267
GsaI	CCCAGC	1 cut(s)	2870
GsuI	CTGGAG	3 cut(s)	382, 1115, 1752
HaeIII	GGCC	8 cut(s)	29, 561, 1557, 1983, 2223, 2585, 2633, 2829
HapII	CCGG	4 cut(s)	14, 102, 1201, 2830
HgaI	GACGC	1 cut(s)	1289
HincII	GTYRAC	2 cut(s)	469, 2896
HindII	GTYRAC	2 cut(s)	469, 2896
HindIII	AAGCTT	3 cut(s)	732, 841, 1235
HpaII	CCGG	4 cut(s)	14, 102, 1201, 2830
HphI	GGTGA	7 cut(s)	116, 515, 931, 1577, 2809, 2885, 2996
Hpy166II	GTNNAC	5 cut(s)	107, 469, 1429, 1739, 2896
Hpy8I	GTNNAC	5 cut(s)	107, 469, 1429, 1739, 2896
HpyCH4III	ACNGT	6 cut(s)	419, 1156, 1391, 1837, 2002, 3138
HpyCH4IV	ACGT	6 cut(s)	177, 335, 1867, 2303, 2308, 3111
HpyF10VI	GCNNNNNNNGC	9 cut(s)	267, 823, 1195, 1265, 1337, 2051, 2093, 2582, 3222
HpyF3I	CTNAG	9 cut(s)	216, 549, 844, 1524, 2460, 2517, 2698, 2747, 3155
HpySE526I	ACGT	6 cut(s)	177, 335, 1867, 2303, 2308, 3111
Ksp22I	TGATCA	1 cut(s)	2859
LguI	GCTCTTC	1 cut(s)	164
LmnI	GCTCC	4 cut(s)	244, 901, 937, 1655
Lsp1109I	GCAGC	8 cut(s)	22, 1280, 1422, 2105, 2403, 2657, 3058, 3253
MaeI	CTAG	8 cut(s)	372, 1358, 1853, 2345, 2528, 2654, 2891, 3176
MaeII	ACGT	6 cut(s)	177, 335, 1867, 2303, 2308, 3111
MaeIII	GTNAC	5 cut(s)	439, 1903, 2304, 2755, 2815
MfeI	CAATTG	2 cut(s)	785, 2124
MflI	RGATCY	8 cut(s)	516, 545, 656, 1029, 1849, 2554, 2743, 2905
MhlI	GDGCHC	4 cut(s)	249, 289, 1200, 3002
MlsI	TGGCCA	1 cut(s)	2223
MluI	ACGCGT	1 cut(s)	112
MluNI	TGGCCA	1 cut(s)	2223
MlyI	GAGTC	3 cut(s)	50, 2476, 2891
MmeI	TCCRAC	6 cut(s)	130, 1056, 1557, 2121, 2424, 2936
Mox20I	TGGCCA	1 cut(s)	2223
MroXI	GAANNNNTTC	2 cut(s)	345, 1776
MscI	TGGCCA	1 cut(s)	2223
MslI	CAYNNNNRTG	4 cut(s)	671, 1695, 1715, 2639
Msp20I	TGGCCA	1 cut(s)	2223
MspA1I	CMGCKG	1 cut(s)	1189
MspCI	CTTAAG	1 cut(s)	575
MspI	CCGG	4 cut(s)	14, 102, 1201, 2830
MunI	CAATTG	2 cut(s)	785, 2124
MvnI	CGCG	1 cut(s)	114
MwoI	GCNNNNNNNGC	9 cut(s)	267, 823, 1195, 1265, 1337, 2051, 2093, 2582, 3222
NciI	CCSGG	1 cut(s)	2831
NdeI	CATATG	2 cut(s)	1876, 2622
NheI	GCTAGC	1 cut(s)	3175
NlaIV	GGNNCC	3 cut(s)	592, 1576, 2907
NmeAIII	GCCGAG	1 cut(s)	78
NmuCI	GTSAC	2 cut(s)	2755, 2815
NspI	RCATGY	2 cut(s)	742, 2816
PaeR7I	CTCGAG	2 cut(s)	288, 566
PagI	TCATGA	2 cut(s)	525, 1710
PasI	CCCWGGG	1 cut(s)	3239
PceI	AGGCCT	1 cut(s)	561
PciSI	GCTCTTC	1 cut(s)	164
PcsI	WCGNNNNNNNCGW	1 cut(s)	50
PdmI	GAANNNNTTC	2 cut(s)	345, 1776
PflMI	CCANNNNNTGG	1 cut(s)	277
PfoI	TCCNGGA	3 cut(s)	306, 349, 1092
PkrI	GCNGC	8 cut(s)	12, 1270, 1437, 2095, 2418, 2647, 3073, 3268
PleI	GAGTC	3 cut(s)	49, 2476, 2891
PpsI	GAGTC	3 cut(s)	49, 2476, 2891
Ppu21I	YACGTR	2 cut(s)	2309, 3112
PshBI	ATTAAT	1 cut(s)	990
PsiI	TTATAA	1 cut(s)	627
Psp124BI	GAGCTC	2 cut(s)	249, 3002
Psp1406I	AACGTT	1 cut(s)	2303
PspFI	CCCAGC	1 cut(s)	2866
PspN4I	GGNNCC	3 cut(s)	592, 1576, 2907
PspPI	GGNCC	6 cut(s)	119, 152, 591, 1792, 2827, 3118
PspXI	VCTCGAGB	2 cut(s)	288, 566
PstI	CTGCAG	2 cut(s)	828, 1333
PsuI	RGATCY	8 cut(s)	516, 545, 656, 1029, 1849, 2554, 2743, 2905
PvuII	CAGCTG	1 cut(s)	1189
RsaI	GTAC	4 cut(s)	572, 1939, 1996, 2705
RsaNI	GTAC	4 cut(s)	571, 1938, 1995, 2704
RseI	CAYNNNNRTG	4 cut(s)	671, 1695, 1715, 2639
SacI	GAGCTC	2 cut(s)	249, 3002
SalI	GTCGAC	1 cut(s)	2894
SapI	GCTCTTC	1 cut(s)	164
SatI	GCNGC	8 cut(s)	11, 1269, 1436, 2094, 2417, 2646, 3072, 3267
Sau96I	GGNCC	6 cut(s)	119, 152, 591, 1792, 2827, 3118
SchI	GAGTC	3 cut(s)	50, 2476, 2891
SduI	GDGCHC	4 cut(s)	249, 289, 1200, 3002
SfcI	CTRYAG	3 cut(s)	824, 1329, 2001
Sfr274I	CTCGAG	2 cut(s)	288, 566
SgrAI	CRCCGGYG	1 cut(s)	101
SinI	GGWCC	5 cut(s)	119, 152, 591, 1792, 3118
SlaI	CTCGAG	2 cut(s)	288, 566
SmiI	ATTTAAAT	1 cut(s)	886
SmiMI	CAYNNNNRTG	4 cut(s)	671, 1695, 1715, 2639
SmlI	CTYRAG	9 cut(s)	288, 435, 540, 566, 575, 1040, 1459, 2885, 3270
SmoI	CTYRAG	9 cut(s)	288, 435, 540, 566, 575, 1040, 1459, 2885, 3270
SseBI	AGGCCT	1 cut(s)	561
SsiI	CCGC	3 cut(s)	2074, 2497, 3179
SspI	AATATT	1 cut(s)	2965
SspMI	CTAG	8 cut(s)	372, 1358, 1853, 2345, 2528, 2654, 2891, 3176
SstI	GAGCTC	2 cut(s)	249, 3002
StuI	AGGCCT	1 cut(s)	561
StyI	CCWWGG	1 cut(s)	1175
SwaI	ATTTAAAT	1 cut(s)	886
TaaI	ACNGT	6 cut(s)	419, 1156, 1391, 1837, 2002, 3138
TaiI	ACGT	6 cut(s)	180, 338, 1870, 2306, 2311, 3114
TatI	WGTACW	1 cut(s)	1994
TscAI	CASTG	6 cut(s)	1735, 1749, 1830, 2138, 2191, 3027
TseFI	GTSAC	2 cut(s)	2755, 2815
TseI	GCWGC	8 cut(s)	10, 1268, 1435, 2093, 2416, 2645, 3071, 3266
Tsp45I	GTSAC	2 cut(s)	2755, 2815
TspGWI	ACGGA	1 cut(s)	1397
TspRI	CASTG	6 cut(s)	1735, 1749, 1830, 2138, 2191, 3027
Van91I	CCANNNNNTGG	1 cut(s)	277
Vha464I	CTTAAG	1 cut(s)	575
VpaK11BI	GGWCC	5 cut(s)	119, 152, 591, 1792, 3118
VspI	ATTAAT	1 cut(s)	990
XapI	RAATTY	6 cut(s)	881, 1208, 1344, 1892, 2314, 2561
XbaI	TCTAGA	1 cut(s)	1852
XceI	RCATGY	2 cut(s)	742, 2816
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2216, 2952
XhoI	CTCGAG	2 cut(s)	288, 566
XmiI	GTMKAC	1 cut(s)	2895
XmnI	GAANNNNTTC	2 cut(s)	345, 1776
XspI	CTAG	8 cut(s)	372, 1358, 1853, 2345, 2528, 2654, 2891, 3176

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.