Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0001236

Physical Location & Seq

Genomic Coordinates Forward (+)

19734020 .. 19744332

Gene Structure 10313 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000001729

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3633 bp

ATGGAATCGCCACCAGACAAGTTCATGGCCGATGGCTCGGAGTCGAGGAAGCCAAGGCTGTTCATCAGGGAAATGGTTATGAGCAACTTCAAGTCTTACGCCGGTGAACAACGCGTTGGTCCTTTCCACAAGAGCTTTTCTGCTGTTGTTGGACCTAATGGGAGTGGGAAGAGCAACGTCATAGATGCAATGCTCTTTGTATTCGGAAAACGAGCTAAGCAGATGCGACTTAACAGAGTTTCGGAGCTCATTCATAACTTCACTAATTGCCACAATATGGATAGTGCTCGAGTTTCTCTTCATTTCCAGGAGATCATTGATTTGGACGATGGGACGTTTGAAGCTGTTCCAGGAAGTGATTTTGTAATCACTAGGGTAGCGTATTGGGATAACTCCTCCAGGTATTACATCAATGACCGTTCAAGCAATTTTACTCAAGTTACCAAGAAACTGAAAGGGAAAGGAGTTGACCTGGACAACAATCGCTTCTTGATTCTTCAGGGTGAAGTTGAGCAGATCTCACTCATGAAGCCAAAAGCTCAAGGATCTCTGGATGAAGGCCTCCTCGAGTACCTTGAGGACATTATTGGGACCAGCAAATATGTCAAAAAAATTGATGAATCTTATAAAGAGATAGAGTCCCTAATTGAGAAAAGATCTGGTGTCGTGCAAATGGTTAAGCTGGCAGAGAAAGAAAGAGATGGCTTGGAGGATGGGAAGAATGAAGCGGAAGCTTACATGCTGAAAGAGTTGTCCCTTCTAAATTGGCAAGAGAAAGCCACACAATTGGCTTATGAAGATACCTTTGCAAAAATGCTTGATCTGCAGGAAACTATAACCAGCTTAGAAGAAAATCTGACCAATGATAGGGAGAAGATCCAGGAGCGTCATAATGCAGTGAAGGAGCTTGAATCTGTGTACAATAAACATATGAAACAACAAGAGGAATTGTATAATACCCTCAACAGATATGAGGATGAATTTAAGCATCTTGAAAAGAAGCGTGCAAAATATCTGGTAAATCTGCAATATGTGGAGCAGAAGATCAAGAAACTCGATGAAAAACTTGAAAAGGATTCATTAAAGATTAATGACAGAGAGAGGGAATGTGAGAACTCGACTAATCAGTTCCCAGAACTTGAGAAAAGTATTCGCCAACTGCGAAAACTCTTGTTGGATGAAGAAAAAGTCCTGGAGGAAATTATTGAGAATTCCAAACGGGATACCGAAACATATCGCTGTGAGCTTACAACAGTTCGTTCCAGATTAGAACCTTGGGAAAAACAACTGATTGAGCACCAGAAGAAATTTGATGTTGCATATACTGAACAGAAGCTTTTGGATGAAAAGCATCAAGCTGGTCGTGCAGCTTTTGAAGACGCTCGAAAGCAGATGGATGATATATTGGGAATGATACAAACAAAAGCTGCAGACATTGCAAAAATTCGGTGTGAGCTAGAAAAGAGCAAATTCGAAAGGACGGAATACTGTAAAGCAGAACAGGAATACATCAAAGAACAAGAAGCTCTCATACGTTGTGAACAAGCTGCAAGACAGAAGGTTGCAGAACTCAAGCATCTTCTGGATTCTGAAAAGAGTAGGGGAAGACTTTTAAAAGCAATTTTACAGGCTAAGTTGTCCAACCGGATACCGGGAATACATGGCCGACTGGGTGATTTAGGTGCCATTGATGAGCGATATGATGTTGCCATATCAACAGCTTGCCCTGGACTTGATAATATTGTGGTGGAAACAACTGGTGGAGCAATAGCTTGTGTTGAGCTACTCCGAAGAGAGCATCTTGGTGTTGCTAATTTCATGATAATGGAAAAGATCACTGATTGTTTACCAGTGAGGAAGGAGAATGTAAGAACTCCAGAAGGAGTTCCACGCCTTTTCGATCTAGTTAGGGTTGAGGACCAGAGAATGAAACTTGCTTTCTTTGAGGTACTGAAGAATACTGTTGTAGCTAAAGATCTAGATCAGGCAGAACGTATTGCATATGGAGGAAACCCCAAATTTCCAGTGGTAACACTTGATGGTGCAGTATTTGAAATATCTGGTACTATGAGTGGTGGTGGAAGTAAGCCATGTGGTAGTAAGATTGGCCGATCTATATGTACTACTGTAGTGTCAGAAGAAGCTATTACAGATGCCAGCAGGGGGCTTGCCAGTATGGCTGATAAATTAAACGATATCCGCCAACATATTGCTGATGCAGCAAAGAGATTTCAAGCCTCTGAAGATGCAATTGCACACTGGGAGTTGGAATTAGCTAAAAGCGAGAAAGAGATTGACAGTTTGAGTTCACAACATAGTTATCTTGAAAAGCAAATTGGTTCCTTGGAGGCTGCATCACATATCATTTCAGTTGAAGGAAATAAGATCGACGAACTCATCCAAGGATCTAAGCAGCTTAAGGAAAAGGCCCTGGAGCTTCAACATAAAATAGAAAATGCTGGTGGTGAAAGATTAAAAGTACAGAAGTCAAAGGTCGATAATATTAAGTCCGATATTGATGAAAAGAACACTGAAATCAACCACCACAAAATCCAGATTGAGACTGGCCAGAAAACTATAAAGGAGTTGAGAAAAGTGATTCAAGATTCAAAGAAGAAGAAAGAACGACATGACAAGGAGAAGGAAACGTTATGTGAAAATTTCAAAGAAAAAGAAGAGAAGGCATCTCTAGTTCAAGAGAACTACGAGGAAATACAGAAGCTGATTGATAAACATAAAGACGTGTTGGATAGAGCAAAATCTGAGTATTGCAATATGAAAAAGACTCTGTATGAATTGCGGGCATGGAAGGATAAAGCTGAGTTTGAACAAGAAGATATGAAGAAGAAATACAAGGATCTGAAATTTGAGGGGAAGGCTTACAAGGCCAGGCTTGATAAGTTGCAAATTGCCCTGATAAAGCATATGGATCAAATTCATAAAGATTTAGTGGACTCTGAAAAGGTTCAAGCAACCTTGGCAGATGAGCATCTTAGTTGTTGCGACCTTAAAAGGGCCATTGAAATGGCAGCACTACTAGAAGCACAAATGAGAGAGATGAATCCAAATCTTGATTCATTCTCAGAGTACCAAAGAAAGGTCTTATTATATAACGAAAGAGTGGAGGATCTGAGTATTGTCACAAAACAGCGTGATGACATAAAGAGGCAGTATGATGAATTGAGAAAGAAGAGGTTGGATGACTTTATGACAGGATTTAATGCAATATCTATGAAGTTAAAGGAAATGTATCAGATGATCACGCTGGGAGGTGATGCAAAACTTGAGCTAGTCGACTCCCTGGATCCTTTCTCCGAAGGTGTTGTCTTCAGCGTAAGACCACCAAAGAAGAGTTGGAAAAATATTTCAAACTTATCTGGTGGTGAAAAGACATTGAGCTCATTGGCTCTCGTGTTTGCACTGCATCATTACAAGCCTACGCCTCTTTATGTCATGGATGAAATTGATGCTGCCTTAGACTTTAAGAATGTCTCCATCGTTGGTCATTACGTGAAGGACCAGACAAAGGATGCACAGTTCATAATCATAAGATCGCTGATGGCGGTTTGGCGAAGGCTGCAGGGTGGCCCTCTGCATGGTCCGGCGGTGGTGGCTGTAGGGATTTGGGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000070 GO:0000278 GO:0000280 GO:0000819 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006323 GO:0006996 GO:0007049 GO:0007059 GO:0007076 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016043 GO:0022402 GO:0022414 GO:0030261 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048285 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051276 GO:0061458 GO:0071103 GO:0071840 GO:0098813 GO:0140014 GO:1903047

KEGG Pathways

Metabolic & Signaling

ko04111 map04111

Pfam Domains

Protein Families

SMC_N SMC_hinge

Protein Analysis

1211

Amino Acids

138.91

Weight (kDa)

6.25

Isoelectric Point (pI)

37.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SMC_N	PF02463	21 - 1183	1.1e-59	RecF/RecN/SMC N terminal domain
AAA_29	PF13555	24 - 80	9.8e-06	P-loop containing region of AAA domain
AAA_23	PF13476	25 - 285	5e-09	AAA domain
SMC_hinge	PF06470	551 - 667	2.8e-20	SMC proteins Flexible Hinge Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001236)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48600 AT5G48600
fragaria_vesca	FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_2g39941 FvH4_4g18080 FvH4_5g30910 FvH4_5g30910 FvH4_5g30910 FvH4_6g23280
malus_domestica	MD02G1198100.v1.1
prunus_persica	Prupe.3G267300_v2.0.a1 Prupe.3G267300_v2.0.a1
pyrus_communis	pycom02g16170
rosa_chinensis	RchiOBHm_Chr5g0008421 RchiOBHm_Chr6g0300801 RchiOBHm_Chr7g0223681 RchiOBHm_Chr7g0223741 RchiOBHm_Chr7g0226461
rosa_laevigata	RLG00000001729 RLG00000011300 RLG00000031570
rosa_multiflora	Rmu_sc0001470.1_g000005 Rmu_sc0001903.1_g000024 Rmu_sc0002826.1_g000022 Rmu_sc0008947.1_g000001
rosa_roxburghii	Rroxscaffold_3G00232810 Rroxscaffold_3G00235410 Rroxscaffold_7G00167400
rosa_rugosa	Rorug06G0299200 Rorug07G0218700 Rorug07G0219100 Rorug07G0219200 Rorug07G0237300
rosa_samantha	Rh6AG412200 Rh6BG419400 Rh6CG426900 Rh6DG412200 Rh7AG362000 Rh7AG384400 Rh7AG384500 Rh7BG353400 Rh7BG353500 Rh7BG371100 Rh7CG380600 Rh7CG403700 Rh7CG403800 Rh7DG356900 Rh7DG357400 Rh7DG383200
rosa_wichuraiana	Rw7G030730 Rw7G030770 Rw7G032280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	627
AccB1I	GGYRCC	1 cut(s)	1682
AccB7I	CCANNNNNTGG	1 cut(s)	277
AccI	GTMKAC	1 cut(s)	3294
AccII	CGCG	1 cut(s)	114
AciI	CCGC	5 cut(s)	728, 2200, 2800, 3563, 3605
AclI	AACGTT	1 cut(s)	2648
AclWI	GGATC	8 cut(s)	553, 871, 2413, 2865, 2937, 3135, 3299, 3312
AcoI	YGGCCR	4 cut(s)	27, 1663, 2107, 2566
AcsI	RAATTY	9 cut(s)	980, 1210, 1307, 1443, 1469, 2018, 2659, 2864, 2934
AcuI	CTGAAG	4 cut(s)	482, 1973, 2262, 3313
AfaI	GTAC	7 cut(s)	572, 920, 1950, 2065, 2122, 2481, 3089
AfiI	CCNNNNNNNGG	8 cut(s)	277, 867, 1651, 2163, 3013, 3097, 3526, 3596
AflII	CTTAAG	1 cut(s)	2417
AflIII	ACRYGT	2 cut(s)	112, 2742
AjiI	CACGTC	1 cut(s)	2743
AjuI	GAANNNNNNNTTGG	6 cut(s)	99, 131, 2319, 2351, 3058, 3090
AloI	GAACNNNNNNTCC	2 cut(s)	3521, 3553
Alw21I	GWGCWC	4 cut(s)	249, 289, 1299, 3401
Alw26I	GTCTC	2 cut(s)	2555, 3496
AlwI	GGATC	8 cut(s)	553, 871, 2413, 2865, 2937, 3135, 3299, 3312
AlwNI	CAGNNNCTG	1 cut(s)	2722
Ama87I	CYCGRG	2 cut(s)	288, 566
ApeKI	GCWGC	9 cut(s)	1367, 1427, 1547, 2219, 2351, 2413, 3029, 3470, 3577
ApoI	RAATTY	9 cut(s)	980, 1210, 1307, 1443, 1469, 2018, 2659, 2864, 2934
ArsI	GACNNNNNNTTYG	2 cut(s)	3309, 3341
AseI	ATTAAT	1 cut(s)	1089
Asp700I	GAANNNNTTC	3 cut(s)	345, 1884, 2964
AsuC2I	CCSGG	1 cut(s)	1653
AsuHPI	GGTGA	6 cut(s)	116, 515, 1685, 2477, 3284, 3395
AsuII	TTCGAA	1 cut(s)	1473
AvaI	CYCGRG	2 cut(s)	288, 566
AvaII	GGWCC	6 cut(s)	119, 152, 591, 1918, 3517, 3599
BalI	TGGCCA	1 cut(s)	2568
BamHI	GGATCC	1 cut(s)	3304
BanI	GGYRCC	1 cut(s)	1682
BanII	GRGCYC	2 cut(s)	249, 3401
BarI	GAAGNNNNNNTAC	4 cut(s)	1515, 1547, 2834, 2866
BauI	CACGAG	1 cut(s)	3410
BbsI	GAAGAC	3 cut(s)	1383, 1612, 3319
Bbv12I	GWGCWC	4 cut(s)	249, 289, 1299, 3401
BbvI	GCAGC	9 cut(s)	1379, 1414, 1534, 2231, 2338, 2425, 3041, 3457, 3564
BccI	CCATC	8 cut(s)	26, 323, 695, 707, 1387, 2033, 3503, 3553
BciVI	GTATCC	2 cut(s)	1216, 1641
BclI	TGATCA	1 cut(s)	3258
BcnI	CCSGG	1 cut(s)	1653
BcoDI	GTCTC	2 cut(s)	2555, 3496
BfaI	CTAG	8 cut(s)	372, 1457, 1904, 1979, 2690, 3038, 3290, 3631
BfmI	CTRYAG	5 cut(s)	824, 1428, 2127, 3578, 3615
BfrI	CTTAAG	1 cut(s)	2417
BfuI	GTATCC	2 cut(s)	1216, 1641
BglI	GCCNNNNNGGC	1 cut(s)	2177
BglII	AGATCT	3 cut(s)	516, 656, 1975
BisI	GCNGC	9 cut(s)	1368, 1428, 1548, 2220, 2352, 2414, 3030, 3471, 3578
BlpI	GCTNAGC	1 cut(s)	216
BlsI	GCNGC	9 cut(s)	1369, 1429, 1549, 2221, 2353, 2415, 3031, 3472, 3579
Bme18I	GGWCC	6 cut(s)	119, 152, 591, 1918, 3517, 3599
BmeT110I	CYCGRG	2 cut(s)	288, 566
BmgBI	CACGTC	1 cut(s)	2743
BmiI	GGNNCC	4 cut(s)	592, 1684, 2341, 3306
BmrI	ACTGGG	2 cut(s)	1679, 2269
BmuI	ACTGGG	2 cut(s)	1679, 2269
BpiI	GAAGAC	3 cut(s)	1383, 1612, 3319
BplI	GAGNNNNNCTC	2 cut(s)	503, 535
BpmI	CTGGAG	4 cut(s)	382, 1214, 1860, 2453
Bpu1102I	GCTNAGC	1 cut(s)	216
Bpu14I	TTCGAA	1 cut(s)	1473
BpuEI	CTTGAG	6 cut(s)	420, 525, 596, 1160, 1556, 3305
BpuMI	CCSGG	1 cut(s)	1653
BsaAI	YACGTR	1 cut(s)	3511
BsaBI	GATNNNNATC	2 cut(s)	1980, 2988
BsaJI	CCNNGG	8 cut(s)	53, 1274, 1726, 2343, 2401, 2430, 2976, 3300
BsaWI	WCCGGW	1 cut(s)	1644
Bsc4I	CCNNNNNNNGG	8 cut(s)	277, 867, 1651, 2163, 3013, 3097, 3526, 3596
Bse118I	RCCGGY	1 cut(s)	101
Bse1I	ACTGG	7 cut(s)	1674, 1762, 1850, 2024, 2172, 2264, 2569
Bse3DI	GCAATG	2 cut(s)	195, 1434
Bse8I	GATNNNNATC	2 cut(s)	1980, 2988
BseDI	CCNNGG	8 cut(s)	53, 1274, 1726, 2343, 2401, 2430, 2976, 3300
BseJI	GATNNNNATC	2 cut(s)	1980, 2988
BseLI	CCNNNNNNNGG	8 cut(s)	277, 867, 1651, 2163, 3013, 3097, 3526, 3596
BseMI	GCAATG	2 cut(s)	195, 1434
BseMII	CTCAG	4 cut(s)	2754, 2811, 3096, 3122
BseNI	ACTGG	7 cut(s)	1674, 1762, 1850, 2024, 2172, 2264, 2569
BseRI	GAGGAG	2 cut(s)	385, 554
BseXI	GCAGC	9 cut(s)	1379, 1414, 1534, 2231, 2338, 2425, 3041, 3457, 3564
BseYI	CCCAGC	1 cut(s)	3265
BsgI	GTGCAG	2 cut(s)	1386, 2064
Bsh1236I	CGCG	1 cut(s)	114
BshNI	GGYRCC	1 cut(s)	1682
BsiHKAI	GWGCWC	4 cut(s)	249, 289, 1299, 3401
BsiHKCI	CYCGRG	2 cut(s)	288, 566
BsiSI	CCGG	4 cut(s)	102, 1645, 1652, 3602
BslFI	GGGAC	4 cut(s)	346, 604, 625, 739
BslI	CCNNNNNNNGG	8 cut(s)	277, 867, 1651, 2163, 3013, 3097, 3526, 3596
BsmAI	GTCTC	2 cut(s)	2555, 3496
BsmFI	GGGAC	4 cut(s)	346, 604, 625, 739
BsoBI	CYCGRG	2 cut(s)	288, 566
Bsp119I	TTCGAA	1 cut(s)	1473
Bsp1286I	GDGCHC	4 cut(s)	249, 289, 1299, 3401
Bsp1407I	TGTACA	1 cut(s)	918
Bsp1720I	GCTNAGC	1 cut(s)	216
BspACI	CCGC	5 cut(s)	728, 2200, 2800, 3563, 3605
BspCNI	CTCAG	4 cut(s)	2755, 2812, 3095, 3123
BspFNI	CGCG	1 cut(s)	114
BspHI	TCATGA	2 cut(s)	525, 1818
BspLI	GGNNCC	4 cut(s)	592, 1684, 2341, 3306
BspMAI	CTGCAG	3 cut(s)	828, 1432, 3582
BspPI	GGATC	8 cut(s)	553, 871, 2413, 2865, 2937, 3135, 3299, 3312
BspQI	GCTCTTC	1 cut(s)	164
BspT104I	TTCGAA	1 cut(s)	1473
BspT107I	GGYRCC	1 cut(s)	1682
BspTI	CTTAAG	1 cut(s)	2417
BsrDI	GCAATG	2 cut(s)	195, 1434
BsrFI	RCCGGY	1 cut(s)	101
BsrGI	TGTACA	1 cut(s)	918
BsrI	ACTGG	7 cut(s)	1674, 1762, 1850, 2024, 2172, 2264, 2569
BssAI	RCCGGY	1 cut(s)	101
BssECI	CCNNGG	8 cut(s)	53, 1274, 1726, 2343, 2401, 2430, 2976, 3300
BssSI	CACGAG	1 cut(s)	3410
BssT1I	CCWWGG	5 cut(s)	53, 1274, 2343, 2401, 2976
Bst2BI	CACGAG	1 cut(s)	3410
Bst4CI	ACNGT	7 cut(s)	419, 1255, 1490, 1963, 2128, 2300, 3537
Bst6I	CTCTTC	6 cut(s)	164, 303, 1786, 2670, 3185, 3344
BstAFI	CTTAAG	1 cut(s)	2417
BstAPI	GCANNNNNTGC	1 cut(s)	1436
BstAUI	TGTACA	1 cut(s)	918
BstBAI	YACGTR	1 cut(s)	3511
BstBI	TTCGAA	1 cut(s)	1473
BstC8I	GCNNGC	6 cut(s)	684, 1005, 1723, 2158, 2169, 2802
BstFNI	CGCG	1 cut(s)	114
BstMAI	GTCTC	2 cut(s)	2555, 3496
BstMWI	GCNNNNNNNGC	9 cut(s)	823, 1364, 1436, 2177, 2219, 2885, 2978, 3577, 3611
BstNSI	RCATGY	1 cut(s)	742
BstSFI	CTRYAG	5 cut(s)	824, 1428, 2127, 3578, 3615
BstUI	CGCG	1 cut(s)	114
BstV1I	GCAGC	9 cut(s)	1379, 1414, 1534, 2231, 2338, 2425, 3041, 3457, 3564
BstV2I	GAAGAC	3 cut(s)	1383, 1612, 3319
BstX2I	RGATCY	9 cut(s)	516, 545, 656, 876, 1975, 2405, 2857, 3127, 3304
BstXI	CCANNNNNNTGG	2 cut(s)	787, 3025
BstYI	RGATCY	9 cut(s)	516, 545, 656, 876, 1975, 2405, 2857, 3127, 3304
BsuI	GTATCC	2 cut(s)	1216, 1641
BtrI	CACGTC	1 cut(s)	2743
BtsI	GCAGTG	2 cut(s)	903, 3419
BtsIMutI	CAGTG	7 cut(s)	903, 1836, 1857, 2031, 2257, 2529, 3419
Cac8I	GCNNGC	6 cut(s)	684, 1005, 1723, 2158, 2169, 2802
CaiI	CAGNNNCTG	1 cut(s)	2722
CciI	TCATGA	2 cut(s)	525, 1818
Cfr10I	RCCGGY	1 cut(s)	101
CseI	GACGC	2 cut(s)	875, 1388
Csp6I	GTAC	7 cut(s)	571, 919, 1949, 2064, 2121, 2480, 3088
CviQI	GTAC	7 cut(s)	571, 919, 1949, 2064, 2121, 2480, 3088
DraI	TTTAAA	1 cut(s)	1614
EaeI	YGGCCR	4 cut(s)	27, 1663, 2107, 2566
Eam1104I	CTCTTC	6 cut(s)	164, 303, 1786, 2670, 3185, 3344
EarI	CTCTTC	6 cut(s)	164, 303, 1786, 2670, 3185, 3344
EciI	GGCGGA	1 cut(s)	2189
Ecl136II	GAGCTC	2 cut(s)	247, 3399
Eco130I	CCWWGG	5 cut(s)	53, 1274, 2343, 2401, 2976
Eco147I	AGGCCT	1 cut(s)	561
Eco24I	GRGCYC	2 cut(s)	249, 3401
Eco32I	GATATC	1 cut(s)	2197
Eco47I	GGWCC	6 cut(s)	119, 152, 591, 1918, 3517, 3599
Eco53kI	GAGCTC	2 cut(s)	247, 3399
Eco57I	CTGAAG	4 cut(s)	482, 1973, 2262, 3313
Eco88I	CYCGRG	2 cut(s)	288, 566
EcoICRI	GAGCTC	2 cut(s)	247, 3399
EcoO109I	RGGNCCY	1 cut(s)	2428
EcoRI	GAATTC	1 cut(s)	1210
EcoRV	GATATC	1 cut(s)	2197
EcoT14I	CCWWGG	5 cut(s)	53, 1274, 2343, 2401, 2976
EcoT38I	GRGCYC	2 cut(s)	249, 3401
ErhI	CCWWGG	5 cut(s)	53, 1274, 2343, 2401, 2976
FaqI	GGGAC	4 cut(s)	346, 604, 625, 739
FauI	CCCGC	1 cut(s)	2793
FauNDI	CATATG	3 cut(s)	930, 2002, 2925
FbaI	TGATCA	1 cut(s)	3258
FblI	GTMKAC	1 cut(s)	3294
Fnu4HI	GCNGC	9 cut(s)	1368, 1428, 1548, 2220, 2352, 2414, 3030, 3471, 3578
FriOI	GRGCYC	2 cut(s)	249, 3401
Fsp4HI	GCNGC	9 cut(s)	1368, 1428, 1548, 2220, 2352, 2414, 3030, 3471, 3578
FspBI	CTAG	8 cut(s)	372, 1457, 1904, 1979, 2690, 3038, 3290, 3631
GluI	GCNGC	9 cut(s)	1368, 1428, 1548, 2220, 2352, 2414, 3030, 3471, 3578
GsaI	CCCAGC	1 cut(s)	3269
GsuI	CTGGAG	4 cut(s)	382, 1214, 1860, 2453
HapII	CCGG	4 cut(s)	102, 1645, 1652, 3602
HgaI	GACGC	2 cut(s)	875, 1388
HincII	GTYRAC	2 cut(s)	469, 3295
HindII	GTYRAC	2 cut(s)	469, 3295
HindIII	AAGCTT	2 cut(s)	732, 1334
HpaII	CCGG	4 cut(s)	102, 1645, 1652, 3602
HphI	GGTGA	6 cut(s)	116, 515, 1685, 2477, 3284, 3395
Hpy166II	GTNNAC	8 cut(s)	107, 469, 919, 1541, 1847, 2309, 2953, 3295
Hpy8I	GTNNAC	8 cut(s)	107, 469, 919, 1541, 1847, 2309, 2953, 3295
Hpy99I	CGWCG	1 cut(s)	2393
HpyCH4III	ACNGT	7 cut(s)	419, 1255, 1490, 1963, 2128, 2300, 3537
HpyCH4IV	ACGT	7 cut(s)	177, 335, 1534, 1993, 2648, 2742, 3510
HpyF10VI	GCNNNNNNNGC	9 cut(s)	823, 1364, 1436, 2177, 2219, 2885, 2978, 3577, 3611
HpySE526I	ACGT	7 cut(s)	177, 335, 1534, 1993, 2648, 2742, 3510
Ksp22I	TGATCA	1 cut(s)	3258
LguI	GCTCTTC	1 cut(s)	164
LmnI	GCTCC	6 cut(s)	244, 883, 904, 1036, 1763, 2434
Lsp1109I	GCAGC	9 cut(s)	1379, 1414, 1534, 2231, 2338, 2425, 3041, 3457, 3564
MaeI	CTAG	8 cut(s)	372, 1457, 1904, 1979, 2690, 3038, 3290, 3631
MaeII	ACGT	7 cut(s)	177, 335, 1534, 1993, 2648, 2742, 3510
MaeIII	GTNAC	3 cut(s)	439, 2029, 3139
MfeI	CAATTG	2 cut(s)	785, 2250
MflI	RGATCY	9 cut(s)	516, 545, 656, 876, 1975, 2405, 2857, 3127, 3304
MhlI	GDGCHC	4 cut(s)	249, 289, 1299, 3401
MlsI	TGGCCA	1 cut(s)	2568
MluI	ACGCGT	1 cut(s)	112
MluNI	TGGCCA	1 cut(s)	2568
MlyI	GAGTC	5 cut(s)	50, 647, 2779, 2948, 3290
MmeI	TCCRAC	7 cut(s)	130, 1155, 1665, 2247, 2727, 3177, 3335
Mox20I	TGGCCA	1 cut(s)	2568
MroXI	GAANNNNTTC	3 cut(s)	345, 1884, 2964
MscI	TGGCCA	1 cut(s)	2568
MslI	CAYNNNNRTG	3 cut(s)	1803, 1823, 3023
Msp20I	TGGCCA	1 cut(s)	2568
MspCI	CTTAAG	1 cut(s)	2417
MspI	CCGG	4 cut(s)	102, 1645, 1652, 3602
MunI	CAATTG	2 cut(s)	785, 2250
MvnI	CGCG	1 cut(s)	114
MwoI	GCNNNNNNNGC	9 cut(s)	823, 1364, 1436, 2177, 2219, 2885, 2978, 3577, 3611
NciI	CCSGG	1 cut(s)	1653
NdeI	CATATG	3 cut(s)	930, 2002, 2925
NlaIV	GGNNCC	4 cut(s)	592, 1684, 2341, 3306
NmuCI	GTSAC	1 cut(s)	3139
NspI	RCATGY	1 cut(s)	742
NspV	TTCGAA	1 cut(s)	1473
PaeR7I	CTCGAG	2 cut(s)	288, 566
PagI	TCATGA	2 cut(s)	525, 1818
PceI	AGGCCT	1 cut(s)	561
PciSI	GCTCTTC	1 cut(s)	164
PcsI	WCGNNNNNNNCGW	1 cut(s)	1159
PdmI	GAANNNNTTC	3 cut(s)	345, 1884, 2964
PflMI	CCANNNNNTGG	1 cut(s)	277
PfoI	TCCNGGA	4 cut(s)	306, 349, 879, 1191
PkrI	GCNGC	9 cut(s)	1369, 1429, 1549, 2221, 2353, 2415, 3031, 3472, 3579
PleI	GAGTC	5 cut(s)	49, 646, 2779, 2948, 3290
PpsI	GAGTC	5 cut(s)	49, 646, 2779, 2948, 3290
Ppu21I	YACGTR	1 cut(s)	3511
PshBI	ATTAAT	1 cut(s)	1089
PsiI	TTATAA	1 cut(s)	627
Psp124BI	GAGCTC	2 cut(s)	249, 3401
Psp1406I	AACGTT	1 cut(s)	2648
PspFI	CCCAGC	1 cut(s)	3265
PspN4I	GGNNCC	4 cut(s)	592, 1684, 2341, 3306
PspXI	VCTCGAGB	2 cut(s)	288, 566
PstI	CTGCAG	3 cut(s)	828, 1432, 3582
PstNI	CAGNNNCTG	1 cut(s)	2722
PsuI	RGATCY	9 cut(s)	516, 545, 656, 876, 1975, 2405, 2857, 3127, 3304
RsaI	GTAC	7 cut(s)	572, 920, 1950, 2065, 2122, 2481, 3089
RsaNI	GTAC	7 cut(s)	571, 919, 1949, 2064, 2121, 2480, 3088
RseI	CAYNNNNRTG	3 cut(s)	1803, 1823, 3023
SacI	GAGCTC	2 cut(s)	249, 3401
SalI	GTCGAC	1 cut(s)	3293
SapI	GCTCTTC	1 cut(s)	164
SatI	GCNGC	9 cut(s)	1368, 1428, 1548, 2220, 2352, 2414, 3030, 3471, 3578
SchI	GAGTC	5 cut(s)	50, 647, 2779, 2948, 3290
SduI	GDGCHC	4 cut(s)	249, 289, 1299, 3401
SfcI	CTRYAG	5 cut(s)	824, 1428, 2127, 3578, 3615
Sfr274I	CTCGAG	2 cut(s)	288, 566
SfuI	TTCGAA	1 cut(s)	1473
SgrAI	CRCCGGYG	1 cut(s)	101
SinI	GGWCC	6 cut(s)	119, 152, 591, 1918, 3517, 3599
SlaI	CTCGAG	2 cut(s)	288, 566
SmiMI	CAYNNNNRTG	3 cut(s)	1803, 1823, 3023
SmlI	CTYRAG	9 cut(s)	288, 435, 540, 566, 575, 1139, 1571, 2417, 3284
SmoI	CTYRAG	9 cut(s)	288, 435, 540, 566, 575, 1139, 1571, 2417, 3284
SseBI	AGGCCT	1 cut(s)	561
SsiI	CCGC	5 cut(s)	728, 2200, 2800, 3563, 3605
SspI	AATATT	3 cut(s)	1741, 2503, 3364
SspMI	CTAG	8 cut(s)	372, 1457, 1904, 1979, 2690, 3038, 3290, 3631
SstI	GAGCTC	2 cut(s)	249, 3401
StuI	AGGCCT	1 cut(s)	561
StyI	CCWWGG	5 cut(s)	53, 1274, 2343, 2401, 2976
TaaI	ACNGT	7 cut(s)	419, 1255, 1490, 1963, 2128, 2300, 3537
TaiI	ACGT	7 cut(s)	180, 338, 1537, 1996, 2651, 2745, 3513
TatI	WGTACW	3 cut(s)	918, 2120, 2479
TscAI	CASTG	7 cut(s)	903, 1843, 1857, 2031, 2264, 2536, 3426
TseFI	GTSAC	1 cut(s)	3139
TseI	GCWGC	9 cut(s)	1367, 1427, 1547, 2219, 2351, 2413, 3029, 3470, 3577
Tsp45I	GTSAC	1 cut(s)	3139
TspGWI	ACGGA	1 cut(s)	1496
TspRI	CASTG	7 cut(s)	903, 1843, 1857, 2031, 2264, 2536, 3426
Van91I	CCANNNNNTGG	1 cut(s)	277
Vha464I	CTTAAG	1 cut(s)	2417
VpaK11BI	GGWCC	6 cut(s)	119, 152, 591, 1918, 3517, 3599
VspI	ATTAAT	1 cut(s)	1089
XapI	RAATTY	9 cut(s)	980, 1210, 1307, 1443, 1469, 2018, 2659, 2864, 2934
XbaI	TCTAGA	1 cut(s)	1978
XceI	RCATGY	1 cut(s)	742
XcmI	CCANNNNNNNNNTGG	3 cut(s)	2023, 2561, 3351
XhoI	CTCGAG	2 cut(s)	288, 566
XmiI	GTMKAC	1 cut(s)	3294
XmnI	GAANNNNTTC	3 cut(s)	345, 1884, 2964
XspI	CTAG	8 cut(s)	372, 1457, 1904, 1979, 2690, 3038, 3290, 3631

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.