Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0000745

Physical Location & Seq

Genomic Coordinates Forward (+)

12688322 .. 12701247

Gene Structure 12926 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1129900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1518 bp

ATGAACCCATTTATATCTTTACTTCTATTCCTTCTACCAATCATCTCTCTATTTATCACAACCTCAGTTTCTGGTTCACCCTTGAAAATCCCAAGGCTGAGTCCTACTGGAGGAACATTTGTACATGGAAATTATTTTGCAAATGACCAACCAAAAGCTTTGTCTGCACTTGATCAAAATGATTTTGAAACATTTTACTACACCCAAACACTTGACCATTTCAACTTCAGGCCCGATAGCTTCACTACTTTTCAACAAAGATATCTGATCAACTCCAAGTACTGGGGTGGCTCCAATGTTAGTGCACCAATATTGGCTTACTTGGGCGCAGAAGAATCAATTGACACCGATCTTGCTATTATCGGCTTTCTCAGTGAAAACGCCAATCAGTTCCAAGCTCTCCAGATATTCATTGAGCACCGGTACTATGGGAAATCAATCCCGTTCGGATCAAGGGAAGAAGCATTTAAAAATGCAAGCACTATTGGGTACTTCAATTCAGCCCAGGCAATAGCAGATTATGCAGAGATCCTCATACATGTAAAGAAGCAACTTCACGCTGAAGATTCTCCGGTGATTGTTATCGGTGGATCATACGGTGGAATGCTTGCTTCATGGTTTCGGCTCAAATATCCCCATGTTGCAGTGGGAGCTCTAGCCTCGTCGGCTCCAATTCTTTACTTTGATAACATTATCCCACCAGAAAAGGGATATTATTTCATTGTGACCAAGGATTTTCAAGAAGCGAGTGAGACTTGCTACCAAACAATAAAGAAATCATGGTCTGTAATTGATGAAATTGCCTCTAAGGATGGAGGCCTCTCAATTCTTAGCAAGAAGTTCCACACTTGCAGTTTGTTGAATAATTCTTCTGAGCTGAAGGACTACTTGAAATCAGTGTATGCTCGGGCAGCTCAATACGATAGTCCACCAAGATATCCAGTTACTGTTGTCTGTGGAGGAATTGATGGAGCTTCTTCTGGAAATGACACTCTCAGCAAAATATTTGCAGGCCTTGTTGCATACAGAGGAAACAGGTCGTGCTATGTTAATCAACCCGGAAACCTATCTGAAACAGATATAGGGTGGGGATGGCAGACGTGTAGTGACATGGTGATACCCATAAGCATCAGCAACGGCACCATGTTTCAACCTGACCTGTTCGATCTGGAAGCCTACATCAAATCCTGCAAGGCAAAGTATGGAGTCCCTCCTCGACCACGTTGGGTCACTACGTACTTCGGAGGCCATGATATAAAACTAGCTCTCCACAGGTTTGCTAGTAACATCATTTTCTCCAATGGGCTAAGAGACCCATACAGTAGTGGCGGGGTGTTGGAGGACATATCACGCACTGTTGTTGCCGTCCATGCAAAGAATGGATCTCATTGCTTGGATATACTTCGTGCAAACCACACTACTGATCCAGGCTGGTTGGTGGAGCAGCGAAAAACTGAAGTGCATATCATCAAAGGATGGTTGGCCAAGTACTATGCTGACCTTCAGGCATTCAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001525 GO:0001568 GO:0001944 GO:0002155 GO:0002254 GO:0002353 GO:0002526 GO:0003008 GO:0003013 GO:0003073 GO:0003085 GO:0003674 GO:0003824 GO:0004177 GO:0005575 GO:0005623 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006954 GO:0007275 GO:0008015 GO:0008150 GO:0008152 GO:0008217 GO:0008233 GO:0008238 GO:0008239 GO:0009611 GO:0009653 GO:0009966 GO:0010594 GO:0010632 GO:0010646 GO:0016787 GO:0019222 GO:0019538 GO:0023051 GO:0030334 GO:0031323 GO:0032501 GO:0032502 GO:0032879 GO:0033500 GO:0035239 GO:0035295 GO:0040012 GO:0042060 GO:0042592 GO:0042593 GO:0043170 GO:0043535 GO:0044238 GO:0044464 GO:0045178 GO:0045776 GO:0048514 GO:0048583 GO:0048646 GO:0048731 GO:0048856 GO:0048871 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051239 GO:0051270 GO:0060055 GO:0065007 GO:0065008 GO:0070011 GO:0071704 GO:0072358 GO:0072359 GO:0072376 GO:0097009 GO:0140096 GO:1901564 GO:2000145 GO:2000377

KEGG Pathways

Metabolic & Signaling

ko04614 ko04974 map04614 map04974

Pfam Domains

Protein Families

Peptidase_S28

Protein Analysis

506

Amino Acids

56.14

Weight (kDa)

6.67

Isoelectric Point (pI)

36.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S28	PF05577	69 - 484	6.4e-69	Serine carboxypeptidase S28

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000745)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22860 AT5G22860 AT5G22860 AT5G22860 AT5G22860 AT5G22860 AT5G22860 AT5G22860
fragaria_vesca	FvH4_3g30250 FvH4_3g30280 FvH4_3g30360 FvH4_3g30360
malus_domestica	MD03G1129900.v1.1 MD03G1130100.v1.1 MD11G1151900.v1.1 MD11G1152000.v1.1
prunus_persica	Prupe.6G031200_v2.0.a1 Prupe.6G115100_v2.0.a1 Prupe.6G115100_v2.0.a1 Prupe.6G115200_v2.0.a1
pyrus_communis	pycom11g12400 pycom11g12420
rosa_chinensis	RchiOBHm_Chr1g0342691 RchiOBHm_Chr1g0342711 RchiOBHm_Chr5g0056161 RchiOBHm_Chr5g0056231 RchiOBHm_Chr5g0056271 RchiOBHm_Chr5g0056301
rosa_laevigata	RLG00000029169 RLG00000029170 RLG00000029188 RLG00000035054 RLG00000035056 RLG00000035068 RLG00000035071 RLG00000035078 RLG00000035081
rosa_multiflora	Rmu_sc0000173.1_g000042 Rmu_sc0000680.1_g000004 Rmu_sc0000680.1_g000008 Rmu_sc0000680.1_g000030 Rmu_sc0001787.1_g000023 Rmu_sc0003586.1_g000001 Rmu_sc0020386.1_g000001
rosa_roxburghii	Rroxscaffold_1G00024030 Rroxscaffold_1G00024080 Rroxscaffold_1G00024190 Rroxscaffold_4G00313170 Rroxscaffold_4G00313200
rosa_rugosa	Rorug01G0146600.1 Rorug05G0297700 Rorug05G0298200 Rorug05G0298300
rosa_samantha	Rh1AG163500 Rh1AG164800 Rh1BG130700 Rh5BG379800 Rh5BG380300 Rh5CG403400 Rh5CG403800 Rh5DG392500 Rh5DG393300 Rh5DG393400
rosa_wichuraiana	Rw0G004400 Rw0G004410 Rw0G004460 Rw0G012640 Rw0G012650 Rw0G012700 Rw0G015550 Rw5G034620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1139
AccB7I	CCANNNNNTGG	1 cut(s)	282
AciI	CCGC	1 cut(s)	1329
AclWI	GGATC	5 cut(s)	457, 523, 598, 1390, 1418
AcoI	YGGCCR	1 cut(s)	1482
AcuI	CTGAAG	5 cut(s)	211, 582, 899, 1476, 1487
AfaI	GTAC	6 cut(s)	123, 281, 425, 491, 1238, 1490
AfiI	CCNNNNNNNGG	3 cut(s)	110, 282, 707
AflIII	ACRYGT	2 cut(s)	538, 1100
AgeI	ACCGGT	1 cut(s)	420
AjiI	CACGTC	1 cut(s)	1101
AjnI	CCWGG	2 cut(s)	504, 1426
AluBI	AGCT	8 cut(s)	158, 240, 398, 653, 877, 914, 974, 1265
AluI	AGCT	8 cut(s)	158, 240, 398, 653, 877, 914, 974, 1265
Alw21I	GWGCWC	3 cut(s)	307, 420, 655
Alw26I	GTCTC	2 cut(s)	746, 1305
Alw44I	GTGCAC	1 cut(s)	303
AlwI	GGATC	5 cut(s)	457, 523, 598, 1390, 1418
AlwNI	CAGNNNCTG	2 cut(s)	71, 947
Ama87I	CYCGRG	1 cut(s)	906
AoxI	GGCC	5 cut(s)	230, 817, 1012, 1246, 1482
ApaLI	GTGCAC	1 cut(s)	303
ApeKI	GCWGC	2 cut(s)	911, 1444
ArsI	GACNNNNNNTTYG	2 cut(s)	146, 178
AsiGI	ACCGGT	1 cut(s)	420
AspLEI	GCGC	1 cut(s)	329
AspS9I	GGNCC	1 cut(s)	231
AsuC2I	CCSGG	1 cut(s)	1059
AsuHPI	GGTGA	3 cut(s)	69, 586, 1126
AvaI	CYCGRG	1 cut(s)	906
BaeGI	GKGCMC	1 cut(s)	307
BalI	TGGCCA	1 cut(s)	1484
BanI	GGYRCC	1 cut(s)	1139
BanII	GRGCYC	1 cut(s)	655
Bbv12I	GWGCWC	3 cut(s)	307, 420, 655
BbvI	GCAGC	2 cut(s)	923, 1456
BccI	CCATC	4 cut(s)	806, 962, 1086, 1470
BceAI	ACGGC	2 cut(s)	1153, 1349
BciT130I	CCWGG	2 cut(s)	506, 1428
BclI	TGATCA	2 cut(s)	172, 267
BcnI	CCSGG	1 cut(s)	1059
BcoDI	GTCTC	2 cut(s)	746, 1305
BfaI	CTAG	3 cut(s)	656, 1262, 1281
BglI	GCCNNNNNGGC	1 cut(s)	665
BisI	GCNGC	2 cut(s)	912, 1445
BlsI	GCNGC	2 cut(s)	913, 1446
BmcAI	AGTACT	2 cut(s)	281, 1490
Bme1390I	CCNGG	3 cut(s)	506, 1059, 1428
BmeT110I	CYCGRG	1 cut(s)	906
BmgBI	CACGTC	1 cut(s)	1101
BmgT120I	GGNCC	1 cut(s)	231
BmiI	GGNNCC	3 cut(s)	292, 669, 1141
BmrFI	CCNGG	3 cut(s)	506, 1059, 1428
BmrI	ACTGGG	1 cut(s)	292
BmsI	GCATC	1 cut(s)	1137
BmuI	ACTGGG	1 cut(s)	292
BpmI	CTGGAG	2 cut(s)	129, 386
BpuMI	CCSGG	1 cut(s)	1059
BsaAI	YACGTR	1 cut(s)	1236
BsaBI	GATNNNNATC	1 cut(s)	581
BsaI	GGTCTC	1 cut(s)	1305
BsaJI	CCNNGG	3 cut(s)	92, 504, 729
BsaWI	WCCGGW	2 cut(s)	420, 571
BsaXI	ACNNNNNCTCC	2 cut(s)	1251, 1281
Bsc4I	CCNNNNNNNGG	3 cut(s)	110, 282, 707
Bse118I	RCCGGY	1 cut(s)	420
Bse1I	ACTGG	3 cut(s)	112, 287, 941
Bse3DI	GCAATG	1 cut(s)	1387
Bse8I	GATNNNNATC	1 cut(s)	581
BseBI	CCWGG	2 cut(s)	506, 1428
BseDI	CCNNGG	3 cut(s)	92, 504, 729
BseGI	GGATG	3 cut(s)	817, 1097, 1481
BseJI	GATNNNNATC	1 cut(s)	581
BseLI	CCNNNNNNNGG	3 cut(s)	110, 282, 707
BseMI	GCAATG	1 cut(s)	1387
BseMII	CTCAG	5 cut(s)	78, 89, 385, 864, 1009
BseNI	ACTGG	3 cut(s)	112, 287, 941
BseRI	GAGGAG	1 cut(s)	1203
BseSI	GKGCMC	1 cut(s)	307
BseXI	GCAGC	2 cut(s)	923, 1456
BsgI	GTGCAG	1 cut(s)	150
BshFI	GGCC	5 cut(s)	232, 819, 1014, 1248, 1484
BshNI	GGYRCC	1 cut(s)	1139
BshTI	ACCGGT	1 cut(s)	420
BsiHKAI	GWGCWC	3 cut(s)	307, 420, 655
BsiHKCI	CYCGRG	1 cut(s)	906
BsiSI	CCGG	3 cut(s)	421, 572, 1059
BslFI	GGGAC	1 cut(s)	1193
BslI	CCNNNNNNNGG	3 cut(s)	110, 282, 707
BsmAI	GTCTC	2 cut(s)	746, 1305
BsmFI	GGGAC	1 cut(s)	1193
BsmI	GAATGC	2 cut(s)	609, 1508
BsnI	GGCC	5 cut(s)	232, 819, 1014, 1248, 1484
Bso31I	GGTCTC	1 cut(s)	1305
BsoBI	CYCGRG	1 cut(s)	906
Bsp1286I	GDGCHC	3 cut(s)	307, 420, 655
Bsp1407I	TGTACA	1 cut(s)	121
Bsp143I	GATC	9 cut(s)	172, 267, 349, 449, 528, 590, 1165, 1382, 1423
BspACI	CCGC	1 cut(s)	1329
BspANI	GGCC	5 cut(s)	232, 819, 1014, 1248, 1484
BspCNI	CTCAG	5 cut(s)	77, 90, 384, 865, 1008
BspLI	GGNNCC	3 cut(s)	292, 669, 1141
BspPI	GGATC	5 cut(s)	457, 523, 598, 1390, 1418
BspT107I	GGYRCC	1 cut(s)	1139
BspTNI	GGTCTC	1 cut(s)	1305
BsrDI	GCAATG	1 cut(s)	1387
BsrFI	RCCGGY	1 cut(s)	420
BsrGI	TGTACA	1 cut(s)	121
BsrI	ACTGG	3 cut(s)	112, 287, 941
BssAI	RCCGGY	1 cut(s)	420
BssECI	CCNNGG	3 cut(s)	92, 504, 729
BssMI	GATC	9 cut(s)	172, 267, 349, 449, 528, 590, 1165, 1382, 1423
BssT1I	CCWWGG	2 cut(s)	92, 729
Bst2UI	CCWGG	2 cut(s)	506, 1428
Bst4CI	ACNGT	4 cut(s)	599, 949, 1322, 1357
BstAPI	GCANNNNNTGC	1 cut(s)	521
BstAUI	TGTACA	1 cut(s)	121
BstBAI	YACGTR	1 cut(s)	1236
BstC8I	GCNNGC	3 cut(s)	478, 609, 1012
BstDEI	CTNAG	8 cut(s)	64, 98, 371, 807, 830, 873, 995, 1307
BstENI	CCTNNNNNAGG	1 cut(s)	108
BstF5I	GGATG	3 cut(s)	817, 1097, 1481
BstHHI	GCGC	1 cut(s)	329
BstKTI	GATC	9 cut(s)	175, 270, 352, 452, 531, 593, 1168, 1385, 1426
BstMAI	GTCTC	2 cut(s)	746, 1305
BstMBI	GATC	9 cut(s)	172, 267, 349, 449, 528, 590, 1165, 1382, 1423
BstMWI	GCNNNNNNNGC	6 cut(s)	164, 521, 650, 665, 911, 1370
BstNI	CCWGG	2 cut(s)	506, 1428
BstNSI	RCATGY	1 cut(s)	542
BstSCI	CCNGG	3 cut(s)	504, 1057, 1426
BstSLI	GKGCMC	1 cut(s)	307
BstSNI	TACGTA	1 cut(s)	1236
BstV1I	GCAGC	2 cut(s)	923, 1456
BstX2I	RGATCY	2 cut(s)	528, 1382
BstYI	RGATCY	2 cut(s)	528, 1382
BsuRI	GGCC	5 cut(s)	232, 819, 1014, 1248, 1484
BtrI	CACGTC	1 cut(s)	1101
BtsCI	GGATG	3 cut(s)	817, 1097, 1481
BtsI	GCAGTG	1 cut(s)	651
BtsIMutI	CAGTG	4 cut(s)	379, 651, 903, 1353
Cac8I	GCNNGC	3 cut(s)	478, 609, 1012
CaiI	CAGNNNCTG	2 cut(s)	71, 947
CfoI	GCGC	1 cut(s)	329
Cfr10I	RCCGGY	1 cut(s)	420
Cfr13I	GGNCC	1 cut(s)	231
Csp6I	GTAC	6 cut(s)	122, 280, 424, 490, 1237, 1489
CspAI	ACCGGT	1 cut(s)	420
CviAII	CATG	9 cut(s)	125, 539, 615, 638, 780, 1111, 1144, 1250, 1370
CviQI	GTAC	6 cut(s)	122, 280, 424, 490, 1237, 1489
DdeI	CTNAG	8 cut(s)	64, 98, 371, 807, 830, 873, 995, 1307
DpnI	GATC	9 cut(s)	174, 269, 351, 451, 530, 592, 1167, 1384, 1425
DpnII	GATC	9 cut(s)	172, 267, 349, 449, 528, 590, 1165, 1382, 1423
DraI	TTTAAA	1 cut(s)	469
EaeI	YGGCCR	1 cut(s)	1482
Ecl136II	GAGCTC	1 cut(s)	653
Eco105I	TACGTA	1 cut(s)	1236
Eco130I	CCWWGG	2 cut(s)	92, 729
Eco147I	AGGCCT	2 cut(s)	819, 1014
Eco24I	GRGCYC	1 cut(s)	655
Eco31I	GGTCTC	1 cut(s)	1305
Eco32I	GATATC	2 cut(s)	263, 938
Eco53kI	GAGCTC	1 cut(s)	653
Eco57I	CTGAAG	5 cut(s)	211, 582, 899, 1476, 1487
Eco88I	CYCGRG	1 cut(s)	906
EcoICRI	GAGCTC	1 cut(s)	653
EcoNI	CCTNNNNNAGG	1 cut(s)	108
EcoRII	CCWGG	2 cut(s)	504, 1426
EcoRV	GATATC	2 cut(s)	263, 938
EcoT14I	CCWWGG	2 cut(s)	92, 729
EcoT38I	GRGCYC	1 cut(s)	655
ErhI	CCWWGG	2 cut(s)	92, 729
FaeI	CATG	9 cut(s)	128, 542, 618, 641, 783, 1114, 1147, 1253, 1373
FalI	AAGNNNNNCTT	2 cut(s)	872, 904
FaqI	GGGAC	1 cut(s)	1193
FatI	CATG	9 cut(s)	124, 538, 614, 637, 779, 1110, 1143, 1249, 1369
FauI	CCCGC	1 cut(s)	1322
FbaI	TGATCA	2 cut(s)	172, 267
Fnu4HI	GCNGC	2 cut(s)	912, 1445
FokI	GGATG	3 cut(s)	824, 1104, 1488
FriOI	GRGCYC	1 cut(s)	655
Fsp4HI	GCNGC	2 cut(s)	912, 1445
FspBI	CTAG	3 cut(s)	656, 1262, 1281
GlaI	GCGC	1 cut(s)	328
GluI	GCNGC	2 cut(s)	912, 1445
GsuI	CTGGAG	2 cut(s)	129, 386
HaeIII	GGCC	5 cut(s)	232, 819, 1014, 1248, 1484
HapII	CCGG	3 cut(s)	421, 572, 1059
HhaI	GCGC	1 cut(s)	329
Hin1II	CATG	9 cut(s)	128, 542, 618, 641, 783, 1114, 1147, 1253, 1373
Hin6I	GCGC	1 cut(s)	327
HinP1I	GCGC	1 cut(s)	327
HindIII	AAGCTT	1 cut(s)	156
HinfI	GANTC	4 cut(s)	100, 335, 566, 1206
HpaII	CCGG	3 cut(s)	421, 572, 1059
HphI	GGTGA	3 cut(s)	69, 586, 1126
Hpy166II	GTNNAC	3 cut(s)	77, 305, 929
Hpy188I	TCNGA	5 cut(s)	267, 449, 874, 1072, 1244
Hpy188III	TCNNGA	4 cut(s)	403, 740, 981, 1169
Hpy8I	GTNNAC	3 cut(s)	77, 305, 929
Hpy99I	CGWCG	1 cut(s)	667
HpyAV	CCTTC	3 cut(s)	41, 874, 1511
HpyCH4III	ACNGT	4 cut(s)	599, 949, 1322, 1357
HpyCH4IV	ACGT	3 cut(s)	1100, 1222, 1235
HpyF10VI	GCNNNNNNNGC	6 cut(s)	164, 521, 650, 665, 911, 1370
HpyF3I	CTNAG	8 cut(s)	64, 98, 371, 807, 830, 873, 995, 1307
HpySE526I	ACGT	3 cut(s)	1100, 1222, 1235
Hsp92II	CATG	9 cut(s)	128, 542, 618, 641, 783, 1114, 1147, 1253, 1373
HspAI	GCGC	1 cut(s)	327
Ksp22I	TGATCA	2 cut(s)	172, 267
Kzo9I	GATC	9 cut(s)	172, 267, 349, 449, 528, 590, 1165, 1382, 1423
LmnI	GCTCC	5 cut(s)	296, 650, 673, 971, 1441
Lsp1109I	GCAGC	2 cut(s)	923, 1456
LweI	GCATC	1 cut(s)	1137
MaeI	CTAG	3 cut(s)	656, 1262, 1281
MaeII	ACGT	3 cut(s)	1100, 1222, 1235
MaeIII	GTNAC	5 cut(s)	724, 943, 1106, 1228, 1283
MalI	GATC	9 cut(s)	174, 269, 351, 451, 530, 592, 1167, 1384, 1425
MboI	GATC	9 cut(s)	172, 267, 349, 449, 528, 590, 1165, 1382, 1423
MboII	GAAGA	5 cut(s)	344, 470, 575, 861, 969
MfeI	CAATTG	1 cut(s)	339
MflI	RGATCY	2 cut(s)	528, 1382
MhlI	GDGCHC	3 cut(s)	307, 420, 655
MlsI	TGGCCA	1 cut(s)	1484
MluCI	AATT	9 cut(s)	130, 339, 496, 672, 789, 798, 825, 865, 963
MluNI	TGGCCA	1 cut(s)	1484
MlyI	GAGTC	2 cut(s)	109, 1215
MmeI	TCCRAC	1 cut(s)	1317
Mox20I	TGGCCA	1 cut(s)	1484
MscI	TGGCCA	1 cut(s)	1484
MseI	TTAA	2 cut(s)	468, 1050
Msp20I	TGGCCA	1 cut(s)	1484
MspI	CCGG	3 cut(s)	421, 572, 1059
MspR9I	CCNGG	3 cut(s)	506, 1059, 1428
MunI	CAATTG	1 cut(s)	339
Mva1269I	GAATGC	2 cut(s)	609, 1508
MvaI	CCWGG	2 cut(s)	506, 1428
MwoI	GCNNNNNNNGC	6 cut(s)	164, 521, 650, 665, 911, 1370
NciI	CCSGG	1 cut(s)	1059
NdeII	GATC	9 cut(s)	172, 267, 349, 449, 528, 590, 1165, 1382, 1423
NlaIII	CATG	9 cut(s)	128, 542, 618, 641, 783, 1114, 1147, 1253, 1373
NlaIV	GGNNCC	3 cut(s)	292, 669, 1141
NmuCI	GTSAC	3 cut(s)	724, 1106, 1228
NspI	RCATGY	1 cut(s)	542
PceI	AGGCCT	2 cut(s)	819, 1014
PciI	ACATGT	1 cut(s)	538
PctI	GAATGC	2 cut(s)	609, 1508
PfeI	GAWTC	2 cut(s)	335, 566
PflMI	CCANNNNNTGG	1 cut(s)	282
PinAI	ACCGGT	1 cut(s)	420
PkrI	GCNGC	2 cut(s)	913, 1446
PleI	GAGTC	2 cut(s)	108, 1214
PpsI	GAGTC	2 cut(s)	108, 1214
Ppu21I	YACGTR	1 cut(s)	1236
PscI	ACATGT	1 cut(s)	538
Psp124BI	GAGCTC	1 cut(s)	655
Psp6I	CCWGG	2 cut(s)	504, 1426
PspGI	CCWGG	2 cut(s)	504, 1426
PspN4I	GGNNCC	3 cut(s)	292, 669, 1141
PspPI	GGNCC	1 cut(s)	231
PstNI	CAGNNNCTG	2 cut(s)	71, 947
PsuI	RGATCY	2 cut(s)	528, 1382
RsaI	GTAC	6 cut(s)	123, 281, 425, 491, 1238, 1490
RsaNI	GTAC	6 cut(s)	122, 280, 424, 490, 1237, 1489
SacI	GAGCTC	1 cut(s)	655
SaqAI	TTAA	2 cut(s)	468, 1050
SatI	GCNGC	2 cut(s)	912, 1445
Sau3AI	GATC	9 cut(s)	172, 267, 349, 449, 528, 590, 1165, 1382, 1423
Sau96I	GGNCC	1 cut(s)	231
ScaI	AGTACT	2 cut(s)	281, 1490
SchI	GAGTC	2 cut(s)	109, 1215
ScrFI	CCNGG	3 cut(s)	506, 1059, 1428
SduI	GDGCHC	3 cut(s)	307, 420, 655
SfaNI	GCATC	1 cut(s)	1137
SnaBI	TACGTA	1 cut(s)	1236
Sse9I	AATT	9 cut(s)	130, 339, 496, 672, 789, 798, 825, 865, 963
SseBI	AGGCCT	2 cut(s)	819, 1014
SsiI	CCGC	1 cut(s)	1329
SspI	AATATT	2 cut(s)	312, 1005
SspMI	CTAG	3 cut(s)	656, 1262, 1281
SstI	GAGCTC	1 cut(s)	655
StuI	AGGCCT	2 cut(s)	819, 1014
StyD4I	CCNGG	3 cut(s)	504, 1057, 1426
StyI	CCWWGG	2 cut(s)	92, 729
TaaI	ACNGT	4 cut(s)	599, 949, 1322, 1357
TaiI	ACGT	3 cut(s)	1103, 1225, 1238
TaqI	TCGA	2 cut(s)	1164, 1216
TasI	AATT	9 cut(s)	130, 339, 496, 672, 789, 798, 825, 865, 963
TatI	WGTACW	3 cut(s)	121, 279, 1488
TfiI	GAWTC	2 cut(s)	335, 566
Tru1I	TTAA	2 cut(s)	468, 1050
Tru9I	TTAA	2 cut(s)	468, 1050
TscAI	CASTG	4 cut(s)	379, 651, 903, 1360
TseFI	GTSAC	3 cut(s)	724, 1106, 1228
TseI	GCWGC	2 cut(s)	911, 1444
Tsp45I	GTSAC	3 cut(s)	724, 1106, 1228
TspDTI	ATGAA	5 cut(s)	17, 400, 603, 709, 810
TspRI	CASTG	4 cut(s)	379, 651, 903, 1360
Van91I	CCANNNNNTGG	1 cut(s)	282
VneI	GTGCAC	1 cut(s)	303
XagI	CCTNNNNNAGG	1 cut(s)	108
XceI	RCATGY	1 cut(s)	542
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1376
XspI	CTAG	3 cut(s)	656, 1262, 1281
ZrmI	AGTACT	2 cut(s)	281, 1490

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.