Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Contig00168

Orthogroup ID

OG0000745

Physical Location & Seq

Genomic Coordinates Forward (+)

81522 .. 84900

Gene Structure 3379 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw0G004410.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1518 bp

ATGAAGCGACTGACATTCTCACTCCAATCATCCCTTCTAGTAATATCTCTTATATTTGTCACAACCTCAGTTTCCTTAAAAATTCCAAGGCTGAGTCCAACTGGAGGGCAATTTATACATGGTAACTATTGGACACAAAGCCGACCCAGCGAAGCTTCATCACCTGCATTTGATCCCAAAGATTTTCAGACATTTTATTACAACCAAACTCTTGACCATTTCAACTTTAGGTATGATAGCTTCAATACTTTTCAACAAAGATATTTGATCAATTCCAAGCATTGGGGTGGCTTCAATATCAGTGCACCAATTCTCGCTTACTTAGGCGCAGAAGAGTCAATTGATGTTGATATACCTATTATTGGGTTCCTACCTGAAAACGCCATTCACTTTCAAGCCCTCCAGATATATATAGAGCACCGGTACTATGGGGAATCAATCCCATTCGGATCCAGGGAAGTGGCATTTAAAAATGCAAGCACTCTTGGATACTTCAACTCAGCCCAAGCTATAGCAGATTATGCAGAAATTCTCATACATGTAAAGAAGCAACTACATGCTGAACATTCTCCGGTCATTGTTATTGGTGGCTCATATGGTGGAATGCTTGCTTCATGGTTTCGACTAAAGTATCCCCACGTCGCTCTTGGAGCTCTGGCCTCATCAGCTCCAATTCTATACTTCGATGACATTGTTCCTCCAGAAACAGGATACTACTCCATTGTCACCAAGGATTTTCAAGAAGCGAGTGAGACTTGCTACCAAATCATAAAAAAGTCTTGGTCTGAAATTGATAATATTGCTTCTAAGCGTGAAGGCCTCTCAAGTCTTAGCAAGAAATTCCAAACTTGCAGACCGTTGACCAAGTCGTCTGAGCTCAAGGACTACTTGAAGTCGATGTATGCTAGGGCAGCTCAATATGATCGTCCACCGAAATATCCGGTTACTGTAGTCTGCGGTGGCATTGATGGAGCTTCATCTACAGGTGATATTTTGACAAAAATATTTGCAGGTGTGGTTGCATATACCGGAAACCGGTCATGCTATGTCAACCGGCCCAGAAACCTAACAGAAACAGACGTGGGGTGGCGATGGCAGACATGTAGCGACTTGGTGATTCCCATAAGCGTGAACAACGACACCATGTTTCCACCGTACAAATTTGATGTCGATGAATATATCAATAAATGCAAGGCAACCTATGGCGTCCCTCCCAGACCTAATTGGGTGACTACATACTTTGGAGGCCATGACATAAAACTGAGTCTCTCCAGATTTGCTAGCAACATCATTTTCTCCAATGGGCTAAGAGACCCTTACAGTATTGGCGGCGTTTTGGAAGACATATCAGACAGTGTGGTTGCTGTCCATGCAAAAAATGGGTCTCACTGCTTGGATATTCTCACCTCAAACCAGACTACCGATCCAGACTGGCTGGTCAATCAGCGGAAAAGTGAGGTCAAAATCATCAAAGGATGGATAGCTAAATACTATGCTGACCTTCAAGCTCTCCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001525 GO:0001568 GO:0001944 GO:0002155 GO:0002254 GO:0002353 GO:0002526 GO:0003008 GO:0003013 GO:0003073 GO:0003085 GO:0003674 GO:0003824 GO:0004177 GO:0005575 GO:0005623 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006954 GO:0007275 GO:0008015 GO:0008150 GO:0008152 GO:0008217 GO:0008233 GO:0008238 GO:0008239 GO:0009611 GO:0009653 GO:0009966 GO:0010594 GO:0010632 GO:0010646 GO:0016787 GO:0019222 GO:0019538 GO:0023051 GO:0030334 GO:0031323 GO:0032501 GO:0032502 GO:0032879 GO:0033500 GO:0035239 GO:0035295 GO:0040012 GO:0042060 GO:0042592 GO:0042593 GO:0043170 GO:0043535 GO:0044238 GO:0044464 GO:0045178 GO:0045776 GO:0048514 GO:0048583 GO:0048646 GO:0048731 GO:0048856 GO:0048871 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051239 GO:0051270 GO:0060055 GO:0065007 GO:0065008 GO:0070011 GO:0071704 GO:0072358 GO:0072359 GO:0072376 GO:0097009 GO:0140096 GO:1901564 GO:2000145 GO:2000377

KEGG Pathways

Metabolic & Signaling

ko04614 ko04974 map04614 map04974

Pfam Domains

Protein Families

Peptidase_S28

Protein Analysis

505

Amino Acids

56.75

Weight (kDa)

7.6

Isoelectric Point (pI)

42.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S28	PF05577	69 - 484	1.1e-70	Serine carboxypeptidase S28

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000745)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22860 AT5G22860 AT5G22860 AT5G22860 AT5G22860 AT5G22860 AT5G22860 AT5G22860
fragaria_vesca	FvH4_3g30250 FvH4_3g30280 FvH4_3g30360 FvH4_3g30360
malus_domestica	MD03G1129900.v1.1 MD03G1130100.v1.1 MD11G1151900.v1.1 MD11G1152000.v1.1
prunus_persica	Prupe.6G031200_v2.0.a1 Prupe.6G115100_v2.0.a1 Prupe.6G115100_v2.0.a1 Prupe.6G115200_v2.0.a1
pyrus_communis	pycom11g12400 pycom11g12420
rosa_chinensis	RchiOBHm_Chr1g0342691 RchiOBHm_Chr1g0342711 RchiOBHm_Chr5g0056161 RchiOBHm_Chr5g0056231 RchiOBHm_Chr5g0056271 RchiOBHm_Chr5g0056301
rosa_laevigata	RLG00000029169 RLG00000029170 RLG00000029188 RLG00000035054 RLG00000035056 RLG00000035068 RLG00000035071 RLG00000035078 RLG00000035081
rosa_multiflora	Rmu_sc0000173.1_g000042 Rmu_sc0000680.1_g000004 Rmu_sc0000680.1_g000008 Rmu_sc0000680.1_g000030 Rmu_sc0001787.1_g000023 Rmu_sc0003586.1_g000001 Rmu_sc0020386.1_g000001
rosa_roxburghii	Rroxscaffold_1G00024030 Rroxscaffold_1G00024080 Rroxscaffold_1G00024190 Rroxscaffold_4G00313170 Rroxscaffold_4G00313200
rosa_rugosa	Rorug01G0146600.1 Rorug05G0297700 Rorug05G0298200 Rorug05G0298300
rosa_samantha	Rh1AG163500 Rh1AG164800 Rh1BG130700 Rh5BG379800 Rh5BG380300 Rh5CG403400 Rh5CG403800 Rh5DG392500 Rh5DG393300 Rh5DG393400
rosa_wichuraiana	Rw0G004400 Rw0G004410 Rw0G004460 Rw0G012640 Rw0G012650 Rw0G012700 Rw0G015550 Rw5G034620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	172, 1001
AasI	GACNNNNNNGTC	2 cut(s)	868, 1436
Acc36I	ACCTGC	2 cut(s)	172, 1001
AccB7I	CCANNNNNTGG	1 cut(s)	282
AciI	CCGC	3 cut(s)	957, 1329, 1447
AclWI	GGATC	4 cut(s)	167, 444, 457, 1418
AcsI	RAATTY	4 cut(s)	81, 528, 839, 1160
AcyI	GRCGYC	1 cut(s)	1206
AfaI	GTAC	2 cut(s)	425, 1157
AfiI	CCNNNNNNNGG	5 cut(s)	104, 282, 362, 707, 1035
AflIII	ACRYGT	2 cut(s)	538, 1100
AgeI	ACCGGT	2 cut(s)	420, 1035
AgsI	TTSAA	9 cut(s)	223, 244, 254, 295, 395, 496, 740, 892, 1505
AjiI	CACGTC	2 cut(s)	640, 1081
AjnI	CCWGG	1 cut(s)	452
AjuI	GAANNNNNNNTTGG	2 cut(s)	18, 50
Alw21I	GWGCWC	4 cut(s)	307, 420, 655, 879
Alw26I	GTCTC	4 cut(s)	746, 1271, 1305, 1389
Alw44I	GTGCAC	1 cut(s)	303
AlwI	GGATC	4 cut(s)	167, 444, 457, 1418
AoxI	GGCC	4 cut(s)	657, 817, 1055, 1246
ApaLI	GTGCAC	1 cut(s)	303
ApeKI	GCWGC	1 cut(s)	911
ApoI	RAATTY	4 cut(s)	81, 528, 839, 1160
ArsI	GACNNNNNNTTYG	4 cut(s)	1152, 1184, 1367, 1399
AsiGI	ACCGGT	2 cut(s)	420, 1035
AspLEI	GCGC	1 cut(s)	329
AspS9I	GGNCC	1 cut(s)	1056
AsuHPI	GGTGA	6 cut(s)	153, 718, 998, 1126, 1240, 1396
AsuNHI	GCTAGC	1 cut(s)	1280
BaeGI	GKGCMC	1 cut(s)	307
BaeI	ACNNNNGTAYC	2 cut(s)	614, 647
BamHI	GGATCC	1 cut(s)	449
BanII	GRGCYC	2 cut(s)	655, 879
BarI	GAAGNNNNNNTAC	2 cut(s)	224, 256
BbsI	GAAGAC	1 cut(s)	1347
Bbv12I	GWGCWC	4 cut(s)	307, 420, 655, 879
BbvI	GCAGC	1 cut(s)	923
BccI	CCATC	3 cut(s)	962, 1086, 1470
BciT130I	CCWGG	1 cut(s)	454
BciVI	GTATCC	3 cut(s)	482, 642, 704
BclI	TGATCA	1 cut(s)	267
BcoDI	GTCTC	4 cut(s)	746, 1271, 1305, 1389
BfaI	CTAG	3 cut(s)	38, 906, 1281
BfmI	CTRYAG	3 cut(s)	510, 948, 981
BfuAI	ACCTGC	2 cut(s)	172, 1001
BfuI	GTATCC	3 cut(s)	482, 642, 704
BisI	GCNGC	2 cut(s)	912, 1330
BlsI	GCNGC	2 cut(s)	913, 1331
Bme1390I	CCNGG	1 cut(s)	454
BmgBI	CACGTC	2 cut(s)	640, 1081
BmgT120I	GGNCC	1 cut(s)	1056
BmiI	GGNNCC	2 cut(s)	368, 451
BmrFI	CCNGG	1 cut(s)	454
BmtI	GCTAGC	1 cut(s)	1284
BpiI	GAAGAC	1 cut(s)	1347
BpmI	CTGGAG	4 cut(s)	123, 386, 684, 1255
BpuEI	CTTGAG	2 cut(s)	808, 863
BsaHI	GRCGYC	1 cut(s)	1206
BsaI	GGTCTC	2 cut(s)	1305, 1389
BsaJI	CCNNGG	3 cut(s)	86, 453, 729
BsaWI	WCCGGW	5 cut(s)	420, 571, 940, 1028, 1035
Bsc4I	CCNNNNNNNGG	5 cut(s)	104, 282, 362, 707, 1035
Bse118I	RCCGGY	3 cut(s)	420, 1035, 1053
Bse1I	ACTGG	2 cut(s)	106, 1436
BseBI	CCWGG	1 cut(s)	454
BseDI	CCNNGG	3 cut(s)	86, 453, 729
BseGI	GGATG	2 cut(s)	29, 1481
BseLI	CCNNNNNNNGG	5 cut(s)	104, 282, 362, 707, 1035
BseMII	CTCAG	5 cut(s)	81, 83, 513, 864, 1253
BseNI	ACTGG	2 cut(s)	106, 1436
BseSI	GKGCMC	1 cut(s)	307
BseXI	GCAGC	1 cut(s)	923
BseYI	CCCAGC	1 cut(s)	146
BshFI	GGCC	4 cut(s)	659, 819, 1057, 1248
BshTI	ACCGGT	2 cut(s)	420, 1035
BsiHKAI	GWGCWC	4 cut(s)	307, 420, 655, 879
BsiSI	CCGG	6 cut(s)	421, 572, 941, 1029, 1036, 1054
BslFI	GGGAC	1 cut(s)	1193
BslI	CCNNNNNNNGG	5 cut(s)	104, 282, 362, 707, 1035
BsmAI	GTCTC	4 cut(s)	746, 1271, 1305, 1389
BsmFI	GGGAC	1 cut(s)	1193
BsmI	GAATGC	1 cut(s)	609
BsnI	GGCC	4 cut(s)	659, 819, 1057, 1248
Bso31I	GGTCTC	2 cut(s)	1305, 1389
Bsp1286I	GDGCHC	4 cut(s)	307, 420, 655, 879
Bsp143I	GATC	5 cut(s)	172, 267, 449, 922, 1423
BspACI	CCGC	3 cut(s)	957, 1329, 1447
BspANI	GGCC	4 cut(s)	659, 819, 1057, 1248
BspCNI	CTCAG	5 cut(s)	80, 84, 512, 865, 1254
BspLI	GGNNCC	2 cut(s)	368, 451
BspMI	ACCTGC	2 cut(s)	172, 1001
BspOI	GCTAGC	1 cut(s)	1284
BspPI	GGATC	4 cut(s)	167, 444, 457, 1418
BspTNI	GGTCTC	2 cut(s)	1305, 1389
BsrFI	RCCGGY	3 cut(s)	420, 1035, 1053
BsrI	ACTGG	2 cut(s)	106, 1436
BssAI	RCCGGY	3 cut(s)	420, 1035, 1053
BssECI	CCNNGG	3 cut(s)	86, 453, 729
BssMI	GATC	5 cut(s)	172, 267, 449, 922, 1423
BssNI	GRCGYC	1 cut(s)	1206
BssT1I	CCWWGG	2 cut(s)	86, 729
Bst2UI	CCWGG	1 cut(s)	454
Bst4CI	ACNGT	5 cut(s)	858, 949, 1155, 1322, 1355
Bst6I	CTCTTC	1 cut(s)	327
BstACI	GRCGYC	1 cut(s)	1206
BstAPI	GCANNNNNTGC	1 cut(s)	521
BstC8I	GCNNGC	3 cut(s)	478, 609, 1282
BstDEI	CTNAG	9 cut(s)	67, 92, 322, 499, 807, 830, 873, 1262, 1307
BstF5I	GGATG	2 cut(s)	29, 1481
BstHHI	GCGC	1 cut(s)	329
BstKTI	GATC	5 cut(s)	175, 270, 452, 925, 1426
BstMAI	GTCTC	4 cut(s)	746, 1271, 1305, 1389
BstMBI	GATC	5 cut(s)	172, 267, 449, 922, 1423
BstMWI	GCNNNNNNNGC	6 cut(s)	147, 521, 650, 665, 911, 1370
BstNI	CCWGG	1 cut(s)	454
BstNSI	RCATGY	3 cut(s)	542, 560, 1104
BstSCI	CCNGG	1 cut(s)	452
BstSFI	CTRYAG	3 cut(s)	510, 948, 981
BstSLI	GKGCMC	1 cut(s)	307
BstV1I	GCAGC	1 cut(s)	923
BstV2I	GAAGAC	1 cut(s)	1347
BstX2I	RGATCY	1 cut(s)	449
BstXI	CCANNNNNNTGG	1 cut(s)	460
BstYI	RGATCY	1 cut(s)	449
BsuI	GTATCC	3 cut(s)	482, 642, 704
BsuRI	GGCC	4 cut(s)	659, 819, 1057, 1248
BtgZI	GCGATG	1 cut(s)	1105
BtrI	CACGTC	2 cut(s)	640, 1081
BtsCI	GGATG	2 cut(s)	29, 1481
BtsI	GCAGTG	1 cut(s)	1387
BtsIMutI	CAGTG	3 cut(s)	307, 1360, 1387
BveI	ACCTGC	2 cut(s)	172, 1001
Cac8I	GCNNGC	3 cut(s)	478, 609, 1282
CfoI	GCGC	1 cut(s)	329
Cfr10I	RCCGGY	3 cut(s)	420, 1035, 1053
Cfr13I	GGNCC	1 cut(s)	1056
CseI	GACGC	1 cut(s)	1195
Csp6I	GTAC	2 cut(s)	424, 1156
CspAI	ACCGGT	2 cut(s)	420, 1035
CviAII	CATG	9 cut(s)	119, 539, 557, 615, 1041, 1101, 1144, 1250, 1370
CviQI	GTAC	2 cut(s)	424, 1156
DdeI	CTNAG	9 cut(s)	67, 92, 322, 499, 807, 830, 873, 1262, 1307
DpnI	GATC	5 cut(s)	174, 269, 451, 924, 1425
DpnII	GATC	5 cut(s)	172, 267, 449, 922, 1423
DraI	TTTAAA	1 cut(s)	469
DrdI	GACNNNNNNGTC	2 cut(s)	868, 1436
DseDI	GACNNNNNNGTC	2 cut(s)	868, 1436
Eam1104I	CTCTTC	1 cut(s)	327
EarI	CTCTTC	1 cut(s)	327
Ecl136II	GAGCTC	2 cut(s)	653, 877
Eco130I	CCWWGG	2 cut(s)	86, 729
Eco147I	AGGCCT	1 cut(s)	819
Eco24I	GRGCYC	2 cut(s)	655, 879
Eco31I	GGTCTC	2 cut(s)	1305, 1389
Eco53kI	GAGCTC	2 cut(s)	653, 877
EcoICRI	GAGCTC	2 cut(s)	653, 877
EcoRII	CCWGG	1 cut(s)	452
EcoT14I	CCWWGG	2 cut(s)	86, 729
EcoT38I	GRGCYC	2 cut(s)	655, 879
ErhI	CCWWGG	2 cut(s)	86, 729
FaeI	CATG	9 cut(s)	122, 542, 560, 618, 1044, 1104, 1147, 1253, 1373
FalI	AAGNNNNNCTT	4 cut(s)	872, 904, 1300, 1332
FaqI	GGGAC	1 cut(s)	1193
FatI	CATG	9 cut(s)	118, 538, 556, 614, 1040, 1100, 1143, 1249, 1369
FauNDI	CATATG	1 cut(s)	595
FbaI	TGATCA	1 cut(s)	267
Fnu4HI	GCNGC	2 cut(s)	912, 1330
FokI	GGATG	2 cut(s)	16, 1488
FriOI	GRGCYC	2 cut(s)	655, 879
Fsp4HI	GCNGC	2 cut(s)	912, 1330
FspBI	CTAG	3 cut(s)	38, 906, 1281
GlaI	GCGC	1 cut(s)	328
GluI	GCNGC	2 cut(s)	912, 1330
GsaI	CCCAGC	1 cut(s)	150
GsuI	CTGGAG	4 cut(s)	123, 386, 684, 1255
HaeIII	GGCC	4 cut(s)	659, 819, 1057, 1248
HapII	CCGG	6 cut(s)	421, 572, 941, 1029, 1036, 1054
HgaI	GACGC	1 cut(s)	1195
HhaI	GCGC	1 cut(s)	329
Hin1I	GRCGYC	1 cut(s)	1206
Hin1II	CATG	9 cut(s)	122, 542, 560, 618, 1044, 1104, 1147, 1253, 1373
Hin6I	GCGC	1 cut(s)	327
HinP1I	GCGC	1 cut(s)	327
HincII	GTYRAC	2 cut(s)	861, 1051
HindII	GTYRAC	2 cut(s)	861, 1051
HindIII	AAGCTT	1 cut(s)	153
HinfI	GANTC	5 cut(s)	94, 335, 434, 1117, 1264
HpaII	CCGG	6 cut(s)	421, 572, 941, 1029, 1036, 1054
HphI	GGTGA	6 cut(s)	153, 718, 998, 1126, 1240, 1396
Hpy166II	GTNNAC	5 cut(s)	305, 861, 929, 1051, 1132
Hpy188I	TCNGA	5 cut(s)	189, 449, 787, 874, 1351
Hpy188III	TCNNGA	6 cut(s)	212, 403, 701, 740, 1272, 1427
Hpy8I	GTNNAC	5 cut(s)	305, 861, 929, 1051, 1132
Hpy99I	CGWCG	1 cut(s)	644
HpyAV	CCTTC	3 cut(s)	44, 809, 1511
HpyCH4III	ACNGT	5 cut(s)	858, 949, 1155, 1322, 1355
HpyCH4IV	ACGT	2 cut(s)	639, 1080
HpyCH4V	TGCA	9 cut(s)	167, 305, 476, 524, 852, 1010, 1022, 1191, 1373
HpyF10VI	GCNNNNNNNGC	6 cut(s)	147, 521, 650, 665, 911, 1370
HpyF3I	CTNAG	9 cut(s)	67, 92, 322, 499, 807, 830, 873, 1262, 1307
HpySE526I	ACGT	2 cut(s)	639, 1080
Hsp92I	GRCGYC	1 cut(s)	1206
Hsp92II	CATG	9 cut(s)	122, 542, 560, 618, 1044, 1104, 1147, 1253, 1373
HspAI	GCGC	1 cut(s)	327
Ksp22I	TGATCA	1 cut(s)	267
Kzo9I	GATC	5 cut(s)	172, 267, 449, 922, 1423
LmnI	GCTCC	3 cut(s)	650, 673, 971
Lsp1109I	GCAGC	1 cut(s)	923
MaeI	CTAG	3 cut(s)	38, 906, 1281
MaeII	ACGT	2 cut(s)	639, 1080
MaeIII	GTNAC	5 cut(s)	58, 122, 724, 943, 1228
MalI	GATC	5 cut(s)	174, 269, 451, 924, 1425
MboI	GATC	5 cut(s)	172, 267, 449, 922, 1423
MboII	GAAGA	2 cut(s)	344, 1352
MfeI	CAATTG	1 cut(s)	339
MflI	RGATCY	1 cut(s)	449
MhlI	GDGCHC	4 cut(s)	307, 420, 655, 879
MlyI	GAGTC	3 cut(s)	103, 344, 1273
MmeI	TCCRAC	1 cut(s)	122
MseI	TTAA	2 cut(s)	77, 468
MslI	CAYNNNNRTG	2 cut(s)	285, 1127
MspA1I	CMGCKG	1 cut(s)	1447
MspI	CCGG	6 cut(s)	421, 572, 941, 1029, 1036, 1054
MspR9I	CCNGG	1 cut(s)	454
MunI	CAATTG	1 cut(s)	339
Mva1269I	GAATGC	1 cut(s)	609
MvaI	CCWGG	1 cut(s)	454
MwoI	GCNNNNNNNGC	6 cut(s)	147, 521, 650, 665, 911, 1370
NdeI	CATATG	1 cut(s)	595
NdeII	GATC	5 cut(s)	172, 267, 449, 922, 1423
NheI	GCTAGC	1 cut(s)	1280
NlaIII	CATG	9 cut(s)	122, 542, 560, 618, 1044, 1104, 1147, 1253, 1373
NlaIV	GGNNCC	2 cut(s)	368, 451
NmuCI	GTSAC	3 cut(s)	58, 724, 1228
NspI	RCATGY	3 cut(s)	542, 560, 1104
PaqCI	CACCTGC	2 cut(s)	172, 1001
PceI	AGGCCT	1 cut(s)	819
PciI	ACATGT	2 cut(s)	538, 1100
PctI	GAATGC	1 cut(s)	609
PfeI	GAWTC	2 cut(s)	434, 1117
PflFI	GACNNNGTC	1 cut(s)	865
PflMI	CCANNNNNTGG	1 cut(s)	282
PinAI	ACCGGT	2 cut(s)	420, 1035
PkrI	GCNGC	2 cut(s)	913, 1331
PleI	GAGTC	3 cut(s)	102, 343, 1272
PpsI	GAGTC	3 cut(s)	102, 343, 1272
PscI	ACATGT	2 cut(s)	538, 1100
Psp124BI	GAGCTC	2 cut(s)	655, 879
Psp6I	CCWGG	1 cut(s)	452
PspFI	CCCAGC	1 cut(s)	146
PspGI	CCWGG	1 cut(s)	452
PspN4I	GGNNCC	2 cut(s)	368, 451
PspPI	GGNCC	1 cut(s)	1056
PsuI	RGATCY	1 cut(s)	449
PsyI	GACNNNGTC	1 cut(s)	865
RsaI	GTAC	2 cut(s)	425, 1157
RsaNI	GTAC	2 cut(s)	424, 1156
RseI	CAYNNNNRTG	2 cut(s)	285, 1127
SacI	GAGCTC	2 cut(s)	655, 879
SaqAI	TTAA	2 cut(s)	77, 468
SatI	GCNGC	2 cut(s)	912, 1330
Sau3AI	GATC	5 cut(s)	172, 267, 449, 922, 1423
Sau96I	GGNCC	1 cut(s)	1056
SchI	GAGTC	3 cut(s)	103, 344, 1273
ScrFI	CCNGG	1 cut(s)	454
SduI	GDGCHC	4 cut(s)	307, 420, 655, 879
SfcI	CTRYAG	3 cut(s)	510, 948, 981
SmiMI	CAYNNNNRTG	2 cut(s)	285, 1127
SmlI	CTYRAG	2 cut(s)	823, 878
SmoI	CTYRAG	2 cut(s)	823, 878
SseBI	AGGCCT	1 cut(s)	819
SsiI	CCGC	3 cut(s)	957, 1329, 1447
SspI	AATATT	2 cut(s)	799, 1005
SspMI	CTAG	3 cut(s)	38, 906, 1281
SstI	GAGCTC	2 cut(s)	655, 879
StuI	AGGCCT	1 cut(s)	819
StyD4I	CCNGG	1 cut(s)	452
StyI	CCWWGG	2 cut(s)	86, 729
TaaI	ACNGT	5 cut(s)	858, 949, 1155, 1322, 1355
TaiI	ACGT	2 cut(s)	642, 1083
TaqI	TCGA	4 cut(s)	622, 684, 896, 1170
TauI	GCSGC	1 cut(s)	1332
TfiI	GAWTC	2 cut(s)	434, 1117
Tru1I	TTAA	2 cut(s)	77, 468
Tru9I	TTAA	2 cut(s)	77, 468
TscAI	CASTG	3 cut(s)	307, 1360, 1394
TseFI	GTSAC	3 cut(s)	58, 724, 1228
TseI	GCWGC	1 cut(s)	911
Tsp45I	GTSAC	3 cut(s)	58, 724, 1228
TspDTI	ATGAA	5 cut(s)	17, 147, 603, 966, 1188
TspRI	CASTG	3 cut(s)	307, 1360, 1394
Tth111I	GACNNNGTC	1 cut(s)	865
Van91I	CCANNNNNTGG	1 cut(s)	282
VneI	GTGCAC	1 cut(s)	303
XapI	RAATTY	4 cut(s)	81, 528, 839, 1160
XceI	RCATGY	3 cut(s)	542, 560, 1104
XcmI	CCANNNNNNNNNTGG	2 cut(s)	644, 1376
XspI	CTAG	3 cut(s)	38, 906, 1281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.