Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0001363

Physical Location & Seq

Genomic Coordinates Forward (+)

23889306 .. 23891402

Gene Structure 2097 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1117900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1776 bp

ATGCCCTCTTTCTCCTCCCCCTCTCTCCCTCCTCTGACCAAGCCTAAACGCCTCGCCACCACCAAGCCTCCCTGCGTCGTCCAGTCTCTCCTCAAGCTCTGCTCTCAGGGCAATCTCTCGCAAGCCCTCTCCACTCTCGACCTTCTAGTCCAAAAGGGTATCCGCTTACCCGCCAAGACTCTCGCTTTCCTCTTGGAACAGTGCGGCGCCACCAGGTCCCTCCGAGAAGGCAAATGGGTTCACATCCATATGAAGCTCACTGGATTCAAGCACCCCTCTACGTTTTTAGCCAACCATTTGATAAATATGTACTTTAAGTGTGCCGATGATGTAGAAGCCCGCAGGGTGTTTGATAAAATGGCTGTGAGGAATTTGTACTCTTGGAACAATATGCTTTCTGGGTATGCTAAGATGAGGAAGTTGAGTGAGGCCAGGAGTTTGTTTGATAAAATGCCGGAGAAGGACGTCGTGTCGTGGAACACCATGGTCATTGGTTATGCCCAGAGTGGAGTTTGTGATGAGGCTTTGAGGTTTTATAGAGAGTTGAGAAGGTTGTCAATTGGTTTCAATGAGCAAGTTCATGCGCAGGTTTTGGTTGCTGGGTTTTTGTCAAATGTTGTGCTTTCTAGTTCGGTTGTTGATGCTTATGCCAAATGCGGGGAGATGGGAGACGCGAGGAAAGTATTCAATAGCATGCATGTGAAGGATGTTCTTGTTTGGACGACACTGGTTTCGGGGTATGCAAAATTTGGTGATATGGAATCAGCTAGTGAATTGTTCAATCGGATGCCCGAGAAGAACCCTGTATCGTGGACAGCTGTGATTTCCGGCTATGCTAGAAATGGTTTGGGGCATGAAGCCCTTGCATTTTTTACAGAGATGATGTTCTATCAAGTTAGACCTGATCAGTTTACGTTTAGTAGTTGCCTTTGTGCTTGTGCTAGTATAGCTTCACTTAAGCATGGTAAACAAGTACATGCGTCTTTAGTACGAAATAATTTTAGACCCAACACAATTGTTGTGAGCTCTCTTATCGACATGTATTCAAAATGTGGGGATTTAGGGGCAGGAAGGCGAGTTTTCGAGCTCATGGGTGATAAGCAAGATACTGTATTATGGAACACGTTGATATCTGCCTTAGCACAGCATGGTAAAGGTATAGAGGCAATGCAGACGTTTGAGGACATGGTCAGATCAGGAATGAAGCCGGATAGGACCACCTTTGTGGTCATTCTCAATGCATGTAGTCATTCTGGTCTAGTGCAGGAAGGGCTTACTTTTTTCGAGTCCATGACTAGTGATCATGGCATTGTTCCTGATGTAGAACATTATGCATGCCTAGTTGATCTCTTGGGTCGAGCTGGACGTTTTGACGAGTTAATCAACCAGCTCAAGAATATGCCAGGTAAAGGTGGGGATCAAGTTTGGAATGCATTACTTGGTGTTAGTAGAATTCATGGAAACACAGAGATGGGAAGAAAAGTGGCTGAACACCTTATTGAGATGGAGCCTCAATCTTCTGGTGCCTATGTATTACTTTCAAGTATATATGCCGAACATGGGAAATGGGAGTTGGTAGAGAAGGTGAGACAGCTTATGGATGAGAGACAAGTGAGGAAAGAGCGAGCCCTTAGTTGGATAGAAGTTGAAAATAGAGTGAATGCTTTCACTGTATTGGATCGGTTGCATCCTCTGAAAGAAGAAATATACTCAGTTTTGGAACAGTTAGCTGGCCAGATGGAAGAAGATGTTTCAGTACCTAATTCCTTGAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

592

Amino Acids

66.23

Weight (kDa)

8.33

Isoelectric Point (pI)

45.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR	PF01535	126 - 155	9.5e-08	PPR repeat
PPR	PF01535	239 - 267	4.7e-07	PPR repeat
PPR_2	PF13041	267 - 314	2.9e-10	PPR repeat family
PPR	PF01535	269 - 295	2e-06	PPR repeat
PPR_2	PF13041	369 - 416	6.9e-13	PPR repeat family
PPR_3	PF13812	369 - 415	3.1e-06	Pentatricopeptide repeat domain
PPR	PF01535	371 - 400	1.9e-07	PPR repeat
E_motif	PF20431	486 - 548	1.7e-16	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001363)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21090
fragaria_vesca	FvH4_1g26660
malus_domestica	MD05G1117900.v1.1 MD10G1120900.v1.1
prunus_persica	Prupe.8G162400_v2.0.a1
pyrus_communis	pycom05g11190 pycom10g10410 pycom10g10420
rosa_chinensis	RchiOBHm_Chr1g0323421 RchiOBHm_Chr1g0323511 RchiOBHm_Chr1g0323621 RchiOBHm_Chr1g0323681 RchiOBHm_Chr1g0323771
rosa_laevigata	RLG00000030297 RLG00000030299 RLG00000030300 RLG00000030302 RLG00000030317 RLG00000030324
rosa_multiflora	Rmu_sc0000252.1_g000001 Rmu_sc0000252.1_g000002 Rmu_sc0000252.1_g000009 Rmu_sc0000252.1_g000012 Rmu_sc0000252.1_g000023 Rmu_sc0000252.1_g000030 Rmu_sc0000804.1_g000006 Rmu_sc0002634.1_g000016 Rmu_sc0002634.1_g000019 Rmu_sc0008902.1_g000002 Rmu_sc0036879.1_g000001 Rmu_sc0036879.1_g000002
rosa_roxburghii	Rroxscaffold_4G00326420 Rroxscaffold_4G00326490 Rroxscaffold_4G00326520 Rroxscaffold_4G00326590
rosa_rugosa	Rorug01G0038100 Rorug01G0038200 Rorug01G0038300 Rorug01G0039100.1 Rorug01G0039200 Rorug01G0039500
rosa_samantha	Rh1AG055800 Rh1AG055900 Rh1AG056300 Rh1AG056400 Rh1AG056500 Rh1AG056700 Rh1BG047700 Rh1CG056700 Rh1CG058000 Rh1DG061800
rosa_wichuraiana	Rw1G004810 Rw1G004820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	146
AatII	GACGTC	1 cut(s)	468
Acc16I	TGCGCA	1 cut(s)	585
Acc36I	ACCTGC	1 cut(s)	577
AccB1I	GGYRCC	2 cut(s)	206, 1523
AccII	CGCG	1 cut(s)	674
AciI	CCGC	5 cut(s)	163, 171, 204, 340, 657
AclWI	GGATC	2 cut(s)	1425, 1686
AcoI	YGGCCR	1 cut(s)	1732
AcsI	RAATTY	3 cut(s)	370, 746, 1452
AcyI	GRCGYC	2 cut(s)	207, 465
AfaI	GTAC	5 cut(s)	311, 377, 975, 990, 1758
AfiI	CCNNNNNNNGG	4 cut(s)	657, 1409, 1560, 1635
AflII	CTTAAG	1 cut(s)	956
AflIII	ACRYGT	2 cut(s)	1038, 1122
AgsI	TTSAA	7 cut(s)	268, 568, 688, 781, 1047, 1542, 1649
AhdI	GACNNNNNGTC	1 cut(s)	469
AhlI	ACTAGT	1 cut(s)	1295
AjnI	CCWGG	3 cut(s)	212, 431, 1402
AjuI	GAANNNNNNNTTGG	2 cut(s)	1556, 1588
AleI	CACNNNNGTG	1 cut(s)	1223
Alw21I	GWGCWC	2 cut(s)	1028, 1089
Alw26I	GTCTC	4 cut(s)	90, 663, 1582, 1600
AlwI	GGATC	2 cut(s)	1425, 1686
Ama87I	CYCGRG	1 cut(s)	791
AoxI	GGCC	2 cut(s)	429, 1732
ApoI	RAATTY	3 cut(s)	370, 746, 1452
ArsI	GACNNNNNNTTYG	2 cut(s)	715, 747
Asp700I	GAANNNNTTC	2 cut(s)	683, 1664
AspLEI	GCGC	2 cut(s)	209, 586
AspS9I	GGNCC	2 cut(s)	216, 1215
AsuHPI	GGTGA	3 cut(s)	764, 1106, 1597
AvaI	CYCGRG	1 cut(s)	791
AvaII	GGWCC	2 cut(s)	216, 1215
BalI	TGGCCA	1 cut(s)	1734
BanI	GGYRCC	2 cut(s)	206, 1523
BanII	GRGCYC	3 cut(s)	1028, 1089, 1630
Bbv12I	GWGCWC	2 cut(s)	1028, 1089
BccI	CCATC	4 cut(s)	658, 1465, 1498, 1732
BciT130I	CCWGG	3 cut(s)	214, 433, 1404
BciVI	GTATCC	1 cut(s)	170
BclI	TGATCA	2 cut(s)	904, 1300
BcoDI	GTCTC	4 cut(s)	90, 663, 1582, 1600
BcuI	ACTAGT	1 cut(s)	1295
BfaI	CTAG	8 cut(s)	146, 627, 768, 837, 942, 1259, 1296, 1340
BfoI	RGCGCY	1 cut(s)	210
BfrI	CTTAAG	1 cut(s)	956
BfuAI	ACCTGC	1 cut(s)	577
BfuI	GTATCC	1 cut(s)	170
BisI	GCNGC	1 cut(s)	205
BlsI	GCNGC	1 cut(s)	206
Bme1390I	CCNGG	3 cut(s)	214, 433, 1404
Bme18I	GGWCC	2 cut(s)	216, 1215
BmeRI	GACNNNNNGTC	1 cut(s)	469
BmeT110I	CYCGRG	1 cut(s)	791
BmgT120I	GGNCC	2 cut(s)	216, 1215
BmiI	GGNNCC	4 cut(s)	208, 218, 1509, 1525
BmrFI	CCNGG	3 cut(s)	214, 433, 1404
BmsI	GCATC	3 cut(s)	631, 777, 1696
Bpu10I	CCTNAGC	1 cut(s)	1138
BpuEI	CTTGAG	2 cut(s)	77, 1376
BsaHI	GRCGYC	2 cut(s)	207, 465
BsaJI	CCNNGG	1 cut(s)	483
BsaXI	ACNNNNNCTCC	2 cut(s)	52, 82
Bsc4I	CCNNNNNNNGG	4 cut(s)	657, 1409, 1560, 1635
Bse1I	ACTGG	3 cut(s)	82, 265, 732
Bse3DI	GCAATG	1 cut(s)	1173
BseBI	CCWGG	3 cut(s)	214, 433, 1404
BseDI	CCNNGG	1 cut(s)	483
BseGI	GGATG	5 cut(s)	243, 712, 792, 1606, 1687
BseLI	CCNNNNNNNGG	4 cut(s)	657, 1409, 1560, 1635
BseMI	GCAATG	1 cut(s)	1173
BseMII	CTCAG	2 cut(s)	119, 1725
BseNI	ACTGG	3 cut(s)	82, 265, 732
BseRI	GAGGAG	3 cut(s)	4, 21, 80
BseYI	CCCAGC	1 cut(s)	599
BsgI	GTGCAG	1 cut(s)	1283
Bsh1236I	CGCG	1 cut(s)	674
BshFI	GGCC	2 cut(s)	431, 1734
BshNI	GGYRCC	2 cut(s)	206, 1523
BsiHKAI	GWGCWC	2 cut(s)	1028, 1089
BsiHKCI	CYCGRG	1 cut(s)	791
BsiSI	CCGG	3 cut(s)	455, 828, 1208
BslFI	GGGAC	1 cut(s)	202
BslI	CCNNNNNNNGG	4 cut(s)	657, 1409, 1560, 1635
BsmAI	GTCTC	4 cut(s)	90, 663, 1582, 1600
BsmBI	CGTCTC	1 cut(s)	663
BsmFI	GGGAC	1 cut(s)	202
BsmI	GAATGC	2 cut(s)	1435, 1666
BsnI	GGCC	2 cut(s)	431, 1734
BsoBI	CYCGRG	1 cut(s)	791
Bsp1286I	GDGCHC	3 cut(s)	1028, 1089, 1630
Bsp143I	GATC	6 cut(s)	904, 1193, 1300, 1345, 1417, 1678
Bsp19I	CCATGG	1 cut(s)	483
BspACI	CCGC	5 cut(s)	163, 171, 204, 340, 657
BspANI	GGCC	2 cut(s)	431, 1734
BspCNI	CTCAG	2 cut(s)	118, 1724
BspFNI	CGCG	1 cut(s)	674
BspLI	GGNNCC	4 cut(s)	208, 218, 1509, 1525
BspMI	ACCTGC	1 cut(s)	577
BspPI	GGATC	2 cut(s)	1425, 1686
BspT107I	GGYRCC	2 cut(s)	206, 1523
BspTI	CTTAAG	1 cut(s)	956
BsrDI	GCAATG	1 cut(s)	1173
BsrI	ACTGG	3 cut(s)	82, 265, 732
BssECI	CCNNGG	1 cut(s)	483
BssMI	GATC	6 cut(s)	904, 1193, 1300, 1345, 1417, 1678
BssNI	GRCGYC	2 cut(s)	207, 465
BssT1I	CCWWGG	1 cut(s)	483
Bst2UI	CCWGG	3 cut(s)	214, 433, 1404
Bst4CI	ACNGT	4 cut(s)	201, 1111, 1672, 1725
BstACI	GRCGYC	2 cut(s)	207, 465
BstAFI	CTTAAG	1 cut(s)	956
BstC8I	GCNNGC	6 cut(s)	123, 340, 695, 1336, 1626, 1732
BstDEI	CTNAG	5 cut(s)	105, 408, 1138, 1631, 1711
BstDSI	CCRYGG	1 cut(s)	483
BstF5I	GGATG	5 cut(s)	243, 712, 792, 1606, 1687
BstFNI	CGCG	1 cut(s)	674
BstH2I	RGCGCY	1 cut(s)	210
BstHHI	GCGC	2 cut(s)	209, 586
BstKTI	GATC	6 cut(s)	907, 1196, 1303, 1348, 1420, 1681
BstMAI	GTCTC	4 cut(s)	90, 663, 1582, 1600
BstMBI	GATC	6 cut(s)	904, 1193, 1300, 1345, 1417, 1678
BstMWI	GCNNNNNNNGC	3 cut(s)	108, 947, 1270
BstNI	CCWGG	3 cut(s)	214, 433, 1404
BstNSI	RCATGY	6 cut(s)	697, 701, 980, 1042, 1245, 1338
BstSCI	CCNGG	3 cut(s)	212, 431, 1402
BstUI	CGCG	1 cut(s)	674
BstXI	CCANNNNNNTGG	1 cut(s)	1225
BsuI	GTATCC	1 cut(s)	170
BsuRI	GGCC	2 cut(s)	431, 1734
BtgI	CCRYGG	1 cut(s)	483
BtsCI	GGATG	5 cut(s)	243, 712, 792, 1606, 1687
BtsIMutI	CAGTG	4 cut(s)	206, 258, 725, 1668
BveI	ACCTGC	1 cut(s)	577
Cac8I	GCNNGC	6 cut(s)	123, 340, 695, 1336, 1626, 1732
CfoI	GCGC	2 cut(s)	209, 586
Cfr13I	GGNCC	2 cut(s)	216, 1215
CseI	GACGC	3 cut(s)	64, 680, 969
CsiI	ACCWGGT	1 cut(s)	212
Csp6I	GTAC	5 cut(s)	310, 376, 974, 989, 1757
CviQI	GTAC	5 cut(s)	310, 376, 974, 989, 1757
DdeI	CTNAG	5 cut(s)	105, 408, 1138, 1631, 1711
DinI	GGCGCC	1 cut(s)	208
DpnI	GATC	6 cut(s)	906, 1195, 1302, 1347, 1419, 1680
DpnII	GATC	6 cut(s)	904, 1193, 1300, 1345, 1417, 1678
DrdI	GACNNNNNNGTC	1 cut(s)	146
DriI	GACNNNNNGTC	1 cut(s)	469
DseDI	GACNNNNNNGTC	1 cut(s)	146
EaeI	YGGCCR	1 cut(s)	1732
Eam1105I	GACNNNNNGTC	1 cut(s)	469
Ecl136II	GAGCTC	2 cut(s)	1026, 1087
Eco130I	CCWWGG	1 cut(s)	483
Eco24I	GRGCYC	3 cut(s)	1028, 1089, 1630
Eco32I	GATATC	1 cut(s)	1131
Eco47I	GGWCC	2 cut(s)	216, 1215
Eco53kI	GAGCTC	2 cut(s)	1026, 1087
Eco88I	CYCGRG	1 cut(s)	791
EcoICRI	GAGCTC	2 cut(s)	1026, 1087
EcoO109I	RGGNCCY	1 cut(s)	216
EcoRI	GAATTC	1 cut(s)	1452
EcoRII	CCWGG	3 cut(s)	212, 431, 1402
EcoRV	GATATC	1 cut(s)	1131
EcoT14I	CCWWGG	1 cut(s)	483
EcoT22I	ATGCAT	4 cut(s)	699, 1243, 1336, 1435
EcoT38I	GRGCYC	3 cut(s)	1028, 1089, 1630
EgeI	GGCGCC	1 cut(s)	208
EheI	GGCGCC	1 cut(s)	208
ErhI	CCWWGG	1 cut(s)	483
Esp3I	CGTCTC	1 cut(s)	663
FaqI	GGGAC	1 cut(s)	202
FauI	CCCGC	3 cut(s)	178, 347, 650
FauNDI	CATATG	1 cut(s)	249
FbaI	TGATCA	2 cut(s)	904, 1300
Fnu4HI	GCNGC	1 cut(s)	205
FokI	GGATG	5 cut(s)	230, 719, 799, 1613, 1674
FriOI	GRGCYC	3 cut(s)	1028, 1089, 1630
Fsp4HI	GCNGC	1 cut(s)	205
FspBI	CTAG	8 cut(s)	146, 627, 768, 837, 942, 1259, 1296, 1340
FspI	TGCGCA	1 cut(s)	585
GlaI	GCGC	2 cut(s)	208, 585
GluI	GCNGC	1 cut(s)	205
GsaI	CCCAGC	1 cut(s)	603
HaeII	RGCGCY	1 cut(s)	210
HaeIII	GGCC	2 cut(s)	431, 1734
HapII	CCGG	3 cut(s)	455, 828, 1208
HgaI	GACGC	3 cut(s)	64, 680, 969
HhaI	GCGC	2 cut(s)	209, 586
Hin1I	GRCGYC	2 cut(s)	207, 465
Hin6I	GCGC	2 cut(s)	207, 584
HinP1I	GCGC	2 cut(s)	207, 584
HinfI	GANTC	4 cut(s)	178, 264, 761, 1286
HpaII	CCGG	3 cut(s)	455, 828, 1208
HphI	GGTGA	3 cut(s)	764, 1106, 1597
Hpy166II	GTNNAC	4 cut(s)	241, 813, 912, 968
Hpy188I	TCNGA	5 cut(s)	36, 224, 786, 1193, 1695
Hpy188III	TCNNGA	4 cut(s)	137, 1197, 1316, 1393
Hpy8I	GTNNAC	4 cut(s)	241, 813, 912, 968
Hpy99I	CGWCG	2 cut(s)	80, 470
HpyAV	CCTTC	8 cut(s)	152, 221, 454, 543, 697, 1065, 1262, 1576
HpyCH4III	ACNGT	4 cut(s)	201, 1111, 1672, 1725
HpyCH4IV	ACGT	6 cut(s)	281, 465, 914, 1124, 1175, 1366
HpyCH4V	TGCA	9 cut(s)	697, 743, 866, 1171, 1241, 1264, 1334, 1433, 1687
HpyF10VI	GCNNNNNNNGC	3 cut(s)	108, 947, 1270
HpyF3I	CTNAG	5 cut(s)	105, 408, 1138, 1631, 1711
HpySE526I	ACGT	6 cut(s)	281, 465, 914, 1124, 1175, 1366
Hsp92I	GRCGYC	2 cut(s)	207, 465
HspAI	GCGC	2 cut(s)	207, 584
KasI	GGCGCC	1 cut(s)	206
Ksp22I	TGATCA	2 cut(s)	904, 1300
Kzo9I	GATC	6 cut(s)	904, 1193, 1300, 1345, 1417, 1678
LmnI	GCTCC	1 cut(s)	1507
LweI	GCATC	3 cut(s)	631, 777, 1696
MabI	ACCWGGT	1 cut(s)	212
MaeI	CTAG	8 cut(s)	146, 627, 768, 837, 942, 1259, 1296, 1340
MaeII	ACGT	6 cut(s)	281, 465, 914, 1124, 1175, 1366
MalI	GATC	6 cut(s)	906, 1195, 1302, 1347, 1419, 1680
MboI	GATC	6 cut(s)	904, 1193, 1300, 1345, 1417, 1678
MboII	GAAGA	6 cut(s)	808, 1488, 1509, 1712, 1754, 1757
MfeI	CAATTG	2 cut(s)	558, 1014
MhlI	GDGCHC	3 cut(s)	1028, 1089, 1630
MlsI	TGGCCA	1 cut(s)	1734
MluCI	AATT	8 cut(s)	370, 558, 746, 773, 997, 1014, 1452, 1762
MluNI	TGGCCA	1 cut(s)	1734
Mly113I	GGCGCC	1 cut(s)	207
MlyI	GAGTC	2 cut(s)	172, 1295
MmeI	TCCRAC	1 cut(s)	1616
Mox20I	TGGCCA	1 cut(s)	1734
Mph1103I	ATGCAT	4 cut(s)	699, 1243, 1336, 1435
MroXI	GAANNNNTTC	2 cut(s)	683, 1664
MscI	TGGCCA	1 cut(s)	1734
MseI	TTAA	3 cut(s)	315, 957, 1379
MslI	CAYNNNNRTG	4 cut(s)	248, 698, 1223, 1469
Msp20I	TGGCCA	1 cut(s)	1734
MspA1I	CMGCKG	1 cut(s)	818
MspCI	CTTAAG	1 cut(s)	956
MspI	CCGG	3 cut(s)	455, 828, 1208
MspR9I	CCNGG	3 cut(s)	214, 433, 1404
MunI	CAATTG	2 cut(s)	558, 1014
Mva1269I	GAATGC	2 cut(s)	1435, 1666
MvaI	CCWGG	3 cut(s)	214, 433, 1404
MvnI	CGCG	1 cut(s)	674
MwoI	GCNNNNNNNGC	3 cut(s)	108, 947, 1270
NarI	GGCGCC	1 cut(s)	207
NcoI	CCATGG	1 cut(s)	483
NdeI	CATATG	1 cut(s)	249
NdeII	GATC	6 cut(s)	904, 1193, 1300, 1345, 1417, 1678
NlaIV	GGNNCC	4 cut(s)	208, 218, 1509, 1525
NsbI	TGCGCA	1 cut(s)	585
NsiI	ATGCAT	4 cut(s)	699, 1243, 1336, 1435
NspI	RCATGY	6 cut(s)	697, 701, 980, 1042, 1245, 1338
OliI	CACNNNNGTG	1 cut(s)	1223
PaeI	GCATGC	2 cut(s)	697, 1338
PciI	ACATGT	1 cut(s)	1038
PcsI	WCGNNNNNNNCGW	1 cut(s)	1363
PctI	GAATGC	2 cut(s)	1435, 1666
PdmI	GAANNNNTTC	2 cut(s)	683, 1664
PfeI	GAWTC	2 cut(s)	264, 761
PflFI	GACNNNGTC	1 cut(s)	1187
PkrI	GCNGC	1 cut(s)	206
PleI	GAGTC	2 cut(s)	172, 1294
PluTI	GGCGCC	1 cut(s)	210
PpsI	GAGTC	2 cut(s)	172, 1294
PpuMI	RGGWCCY	1 cut(s)	216
PscI	ACATGT	1 cut(s)	1038
Psp124BI	GAGCTC	2 cut(s)	1028, 1089
Psp5II	RGGWCCY	1 cut(s)	216
Psp6I	CCWGG	3 cut(s)	212, 431, 1402
PspFI	CCCAGC	1 cut(s)	599
PspGI	CCWGG	3 cut(s)	212, 431, 1402
PspN4I	GGNNCC	4 cut(s)	208, 218, 1509, 1525
PspPI	GGNCC	2 cut(s)	216, 1215
PspPPI	RGGWCCY	1 cut(s)	216
PsyI	GACNNNGTC	1 cut(s)	1187
PvuII	CAGCTG	1 cut(s)	818
RsaI	GTAC	5 cut(s)	311, 377, 975, 990, 1758
RsaNI	GTAC	5 cut(s)	310, 376, 974, 989, 1757
RseI	CAYNNNNRTG	4 cut(s)	248, 698, 1223, 1469
SacI	GAGCTC	2 cut(s)	1028, 1089
SaqAI	TTAA	3 cut(s)	315, 957, 1379
SatI	GCNGC	1 cut(s)	205
Sau3AI	GATC	6 cut(s)	904, 1193, 1300, 1345, 1417, 1678
Sau96I	GGNCC	2 cut(s)	216, 1215
SchI	GAGTC	2 cut(s)	172, 1295
ScrFI	CCNGG	3 cut(s)	214, 433, 1404
SduI	GDGCHC	3 cut(s)	1028, 1089, 1630
SexAI	ACCWGGT	1 cut(s)	212
SfaNI	GCATC	3 cut(s)	631, 777, 1696
SfoI	GGCGCC	1 cut(s)	208
SinI	GGWCC	2 cut(s)	216, 1215
SmiMI	CAYNNNNRTG	4 cut(s)	248, 698, 1223, 1469
SmlI	CTYRAG	4 cut(s)	92, 956, 1391, 1768
SmoI	CTYRAG	4 cut(s)	92, 956, 1391, 1768
SpeI	ACTAGT	1 cut(s)	1295
SphI	GCATGC	2 cut(s)	697, 1338
Sse9I	AATT	8 cut(s)	370, 558, 746, 773, 997, 1014, 1452, 1762
SsiI	CCGC	5 cut(s)	163, 171, 204, 340, 657
SspDI	GGCGCC	1 cut(s)	206
SspMI	CTAG	8 cut(s)	146, 627, 768, 837, 942, 1259, 1296, 1340
SstI	GAGCTC	2 cut(s)	1028, 1089
StyD4I	CCNGG	3 cut(s)	212, 431, 1402
StyI	CCWWGG	1 cut(s)	483
TaaI	ACNGT	4 cut(s)	201, 1111, 1672, 1725
TaiI	ACGT	6 cut(s)	284, 468, 917, 1127, 1178, 1369
TaqI	TCGA	5 cut(s)	138, 1035, 1083, 1284, 1357
TasI	AATT	8 cut(s)	370, 558, 746, 773, 997, 1014, 1452, 1762
TatI	WGTACW	3 cut(s)	309, 375, 973
TauI	GCSGC	1 cut(s)	207
TfiI	GAWTC	2 cut(s)	264, 761
Tru1I	TTAA	3 cut(s)	315, 957, 1379
Tru9I	TTAA	3 cut(s)	315, 957, 1379
TscAI	CASTG	4 cut(s)	206, 265, 732, 1675
TspDTI	ATGAA	5 cut(s)	266, 569, 870, 1217, 1445
TspRI	CASTG	4 cut(s)	206, 265, 732, 1675
Tth111I	GACNNNGTC	1 cut(s)	1187
Vha464I	CTTAAG	1 cut(s)	956
VpaK11BI	GGWCC	2 cut(s)	216, 1215
XapI	RAATTY	3 cut(s)	370, 746, 1452
XceI	RCATGY	6 cut(s)	697, 701, 980, 1042, 1245, 1338
XmnI	GAANNNNTTC	2 cut(s)	683, 1664
XspI	CTAG	8 cut(s)	146, 627, 768, 837, 942, 1259, 1296, 1340
ZraI	GACGTC	1 cut(s)	466
Zsp2I	ATGCAT	4 cut(s)	699, 1243, 1336, 1435

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.