Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000514

Physical Location & Seq

Genomic Coordinates Forward (+)

27469980 .. 27472553

Gene Structure 2574 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1143500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1770 bp

ATGGACGTCCGAAGGCGATCGACGATGGATACACCTGCCACCAAGGCCAGAAGTGGGCCGATGAAGGTGAAAGTGGTGGACCACGAGGGCGACGTTGGTGTCGTCGGGGCCAAGGCCTCCGACGCCCTGCCGCTGCCGTTGTACCTGACTAACGCCGTCTTCTTCACTCTCTTCTTCTCCGTCGTCTACTTCCTCCTTACTCGTTGGCGCGAGAAGATCAGGACCTCGACGCCGCTCCACGTCGTGAACCTTTCCGAGATCGTCGCGATACTCGCGTTCGTCGCCTCCTTCATCTACTTGCTCGGATTCTTCGGGATCGATTTCGTGCAGTCGCTCATTCTCCGCCCCAGCAATGACGTCTGGGCCGCCGACGATGACGAGGAGGAGCACGAGCGCTTGATATTGAAGGACGACGCCCGGAAAGTGCCGTGTGGGGCCGGACTCGACTGCAGCCCAATTCCCCAAATTGCCCCTGTTGCTGCTGCCGCCCCCAAAGCTGTTGCACAGAAGGTGTTTGATAAAGAGGTAGTCCTCTCCACTCCCTGCGATTTCACCGCCCAGCCGTTGACGGAGGAAGATGAGGAGGTGGTCAAGTCCGTGGTGGCGGGAACCATCCCTTCCTACTCTCTGGAGTCAAAGCTCGGAGATTGCAGGAGGGCGGCGGCTATCAGGCGCGAGGCGCTTCAGAGGATCACAGGAAAGTCTCTGGGTGGTCTGCCATTGGAGGGGTTCGATTACGAATTCCTTATGTCTCGATTGCAGAGCCTCTTATTCCAGAGAGTACCAATGGCCACCACCGAAGGTTGCTTGGTTGCCAGCACCAACCGTGGCTTCAAAGCTATCAACTTGTCCGGCGGAGCCACCAGTGTGTTGCTGAGAGATGGGATGACCAGAGCACCTTGTGTGAGGTTCAACTCTGCTAAGAGAGCTGCCGAGTTGAAGTTCTACTTGGAAGAACCCAACAATTATGACACCTTGTCCACGGTTTTCAACAGGTCAAGCAGATTCGGTAGGCTTCAGACAATTAAGTGTGCCATTGCTGGGAAGAACTTGTACATGAGATTCACCTGCAGCACCGGTGATGCTATGGGGATGAACATGGTCTCCAAAGGTGTGCAAAACGTCTTGGATTTCCTCCAGAACGACTTCCCTGACATGGATGTGATTGGAATTTCCGGCAACTACTGCTCTGACAAGAAGCCCGCTGCGGTGAACTGGATTGAAGGCCGCGGAAAATCGGTGGTCTGTGAGGCTGTGATCAAGGGTGATGTGGTGCAGAAGGTGTTGAAAACCAATGTGGCGTCCCTGTGCGAGCTTAACATGCTCAAGAACCTTACTGGGTCTGCAATGGCTGGAGCCCTCGGTGGATTCAACGCACATGCCAGCAACATCGTCTCCGCCATCTACATCGCTACCGGCCAAGACCCAGCTCAGAATGTGGAGAGTTCTCACTGCATTACCATGATGGAACCCATCAATGATGGACAGGACCTTCACGTGTCTGTCACCATGCCTTCAATTGAGGTTGGTACTGTTGGAGGTGGGACCCAACTTGCATCTCAATCAGCTTGTCTGAACCTTCTTGGAGTGAAGGGTGCTAACAGGGAGGCACCAGGATCAAACGCAAGATTGTTGGCCACTGTTGTGGCTGGTTCTGTTCTTGCTGGAGAGCTTTCTCTCATGTCTGCTATCTCAGCTGGACAGCTTGTGAATAGTCACATGAAATACAACAGATCAAGCAAAGATGTCTCAGCTGTTGCATCCGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004420 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005777 GO:0005778 GO:0005783 GO:0005789 GO:0006629 GO:0006694 GO:0006720 GO:0006721 GO:0006722 GO:0008150 GO:0008152 GO:0008202 GO:0008299 GO:0008610 GO:0009058 GO:0009526 GO:0009536 GO:0009987 GO:0012505 GO:0016020 GO:0016104 GO:0016114 GO:0016125 GO:0016126 GO:0016491 GO:0016614 GO:0016616 GO:0031090 GO:0031903 GO:0031966 GO:0031967 GO:0031975 GO:0031984 GO:0042170 GO:0042175 GO:0042282 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044432 GO:0044435 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0098588 GO:0098805 GO:0098827 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00900 ko01100 ko01110 ko01130 ko04152 ko04976 map00900 map01100 map01110 map01130 map04152 map04976

Pfam Domains

Protein Families

HMG-CoA_red

Protein Analysis

590

Amino Acids

63.15

Weight (kDa)

7.02

Isoelectric Point (pI)

38.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMG-CoA_red	PF00368	261 - 578	3.6e-123	Hydroxymethylglutaryl-coenzyme A reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000514)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17370
fragaria_vesca	FvH4_2g17051 FvH4_2g17060 FvH4_2g17080 FvH4_2g17090 FvH4_2g17100 FvH4_2g17100 FvH4_2g17100 FvH4_2g17100
malus_domestica	MD05G1143500.v1.1 MD10G1143400.v1.1
prunus_persica	Prupe.8G182300_v2.0.a1
pyrus_communis	pycom05g13820 pycom10g12350
rosa_chinensis	RchiOBHm_Chr6g0281271 RchiOBHm_Chr6g0281291 RchiOBHm_Chr6g0281321 RchiOBHm_Chr6g0281411 RchiOBHm_Chr6g0281421 RchiOBHm_Chr6g0281471 RchiOBHm_Chr6g0281491 RchiOBHm_Chr6g0281521
rosa_laevigata	RLG00000012968 RLG00000012970 RLG00000012971 RLG00000012973 RLG00000012974 RLG00000012978 RLG00000012980
rosa_multiflora	Rmu_co8270795.1_g000001 Rmu_co8461535.1_g000001 Rmu_sc0000847.1_g000006 Rmu_sc0000847.1_g000012 Rmu_sc0000847.1_g000019 Rmu_sc0000847.1_g000036 Rmu_sc0000847.1_g000045 Rmu_sc0000847.1_g000047 Rmu_sc0001710.1_g000007 Rmu_sc0001710.1_g000011
rosa_roxburghii	Rroxscaffold_7G00187040 Rroxscaffold_7G00187050 Rroxscaffold_7G00187090 Rroxscaffold_7G00187130 Rroxscaffold_7G00187160 Rroxscaffold_7G00187180
rosa_rugosa	Rorug06G0140200 Rorug06G0140400 Rorug06G0140700 Rorug06G0140800 Rorug06G0140900 Rorug06G0141000 Rorug06G0141100
rosa_samantha	Rh6AG252000 Rh6AG252200 Rh6AG252300 Rh6AG252600 Rh6AG252900 Rh6AG253000 Rh6AG253400 Rh6AG253500 Rh6BG255400 Rh6BG255700 Rh6BG255800 Rh6BG256000 Rh6BG256400 Rh6BG256600 Rh6CG254800 Rh6CG254900 Rh6CG255200 Rh6CG255400 Rh6CG255500 Rh6DG246600 Rh6DG246800 Rh6DG247000 Rh6DG247200 Rh6DG247500 Rh6DG247600 Rh6DG247700
rosa_wichuraiana	Rw6G021890 Rw6G021910 Rw6G021940 Rw6G021980 Rw6G022000 Rw6G022010 Rw6G022020 Rw6G022030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	43, 1076
AasI	GACNNNNNNGTC	1 cut(s)	98
AatII	GACGTC	2 cut(s)	9, 360
Acc36I	ACCTGC	2 cut(s)	43, 1076
AccB1I	GGYRCC	1 cut(s)	1609
AccBSI	CCGCTC	1 cut(s)	235
AccI	GTMKAC	1 cut(s)	186
AccII	CGCG	5 cut(s)	210, 266, 275, 675, 1230
AclWI	GGATC	3 cut(s)	323, 698, 1624
AcoI	YGGCCR	3 cut(s)	789, 1417, 1635
AcsI	RAATTY	2 cut(s)	740, 1170
AcuI	CTGAAG	2 cut(s)	668, 1001
AcvI	CACGTG	1 cut(s)	1498
AcyI	GRCGYC	6 cut(s)	6, 123, 230, 357, 414, 1301
AdeI	CACNNNGTG	2 cut(s)	244, 902
AfaI	GTAC	4 cut(s)	143, 783, 1055, 1531
AfeI	AGCGCT	1 cut(s)	395
AfiI	CCNNNNNNNGG	4 cut(s)	54, 725, 1041, 1156
AflIII	ACRYGT	1 cut(s)	1497
AgeI	ACCGGT	1 cut(s)	1076
AgsI	TTSAA	9 cut(s)	406, 835, 913, 940, 991, 1223, 1288, 1372, 1518
AhdI	GACNNNNNGTC	1 cut(s)	976
AjiI	CACGTC	1 cut(s)	241
AjnI	CCWGG	1 cut(s)	1612
AjuI	GAANNNNNNNTTGG	4 cut(s)	815, 847, 932, 964
AleI	CACNNNNGTG	2 cut(s)	866, 1643
AloI	GAACNNNNNNTCC	2 cut(s)	1088, 1120
Alw21I	GWGCWC	2 cut(s)	390, 898
Alw26I	GTCTC	5 cut(s)	708, 756, 1108, 1399, 1753
AlwI	GGATC	3 cut(s)	323, 698, 1624
Aor51HI	AGCGCT	1 cut(s)	395
ApeKI	GCWGC	7 cut(s)	133, 450, 479, 482, 929, 1071, 1205
ApoI	RAATTY	2 cut(s)	740, 1170
AsiGI	ACCGGT	1 cut(s)	1076
Asp700I	GAANNNNTTC	1 cut(s)	1145
AspLEI	GCGC	4 cut(s)	210, 396, 675, 682
AspS9I	GGNCC	8 cut(s)	56, 79, 108, 222, 363, 435, 1489, 1545
AsuC2I	CCSGG	1 cut(s)	418
AsuHPI	GGTGA	7 cut(s)	79, 544, 1057, 1091, 1222, 1277, 1498
AvaII	GGWCC	4 cut(s)	79, 222, 1489, 1545
BaeI	ACNNNNGTAYC	2 cut(s)	260, 293
BalI	TGGCCA	2 cut(s)	791, 1637
BanI	GGYRCC	1 cut(s)	1609
BanII	GRGCYC	1 cut(s)	1360
BarI	GAAGNNNNNNTAC	2 cut(s)	1037, 1069
BauI	CACGAG	2 cut(s)	83, 389
BbrPI	CACGTG	1 cut(s)	1498
BbsI	GAAGAC	1 cut(s)	151
Bbv12I	GWGCWC	2 cut(s)	390, 898
BbvI	GCAGC	7 cut(s)	120, 462, 466, 469, 916, 1083, 1192
BccI	CCATC	7 cut(s)	19, 620, 875, 1409, 1459, 1475, 1481
BceAI	ACGGC	4 cut(s)	121, 140, 412, 547
BciT130I	CCWGG	1 cut(s)	1614
BciVI	GTATCC	1 cut(s)	22
BclI	TGATCA	1 cut(s)	1257
BcnI	CCSGG	1 cut(s)	418
BcoDI	GTCTC	5 cut(s)	708, 756, 1108, 1399, 1753
BfmI	CTRYAG	2 cut(s)	448, 1069
BfoI	RGCGCY	2 cut(s)	397, 683
BfuAI	ACCTGC	2 cut(s)	43, 1076
BfuI	GTATCC	1 cut(s)	22
BglI	GCCNNNNNGGC	1 cut(s)	44
Bme1390I	CCNGG	2 cut(s)	418, 1614
Bme18I	GGWCC	4 cut(s)	79, 222, 1489, 1545
BmeRI	GACNNNNNGTC	1 cut(s)	976
BmgBI	CACGTC	1 cut(s)	241
BmgT120I	GGNCC	8 cut(s)	56, 79, 108, 222, 363, 435, 1489, 1545
BmiI	GGNNCC	9 cut(s)	109, 436, 610, 859, 1357, 1470, 1546, 1547, 1611
BmrFI	CCNGG	2 cut(s)	418, 1614
BmrI	ACTGGG	1 cut(s)	1347
BmsI	GCATC	2 cut(s)	1072, 1565
BmuI	ACTGGG	1 cut(s)	1347
BpiI	GAAGAC	1 cut(s)	151
BpmI	CTGGAG	4 cut(s)	650, 1121, 1374, 1686
BpuEI	CTTGAG	1 cut(s)	1310
BpuMI	CCSGG	1 cut(s)	418
Bsa29I	ATCGAT	1 cut(s)	318
BsaAI	YACGTR	1 cut(s)	1498
BsaHI	GRCGYC	6 cut(s)	6, 123, 230, 357, 414, 1301
BsaI	GGTCTC	1 cut(s)	1108
BsaJI	CCNNGG	7 cut(s)	42, 111, 597, 826, 981, 1228, 1360
BsaWI	WCCGGW	1 cut(s)	1076
BsaXI	ACNNNNNCTCC	4 cut(s)	1088, 1118, 1379, 1409
Bsc4I	CCNNNNNNNGG	4 cut(s)	54, 725, 1041, 1156
Bse118I	RCCGGY	2 cut(s)	1076, 1415
Bse1I	ACTGG	3 cut(s)	864, 1220, 1342
Bse3DI	GCAATG	3 cut(s)	358, 1035, 1353
BseBI	CCWGG	1 cut(s)	1614
BseCI	ATCGAT	1 cut(s)	318
BseDI	CCNNGG	7 cut(s)	42, 111, 597, 826, 981, 1228, 1360
BseGI	GGATG	5 cut(s)	612, 891, 1098, 1165, 1760
BseLI	CCNNNNNNNGG	4 cut(s)	54, 725, 1041, 1156
BseMI	GCAATG	3 cut(s)	358, 1035, 1353
BseMII	CTCAG	4 cut(s)	866, 1445, 1707, 1764
BseNI	ACTGG	3 cut(s)	864, 1220, 1342
BseRI	GAGGAG	3 cut(s)	395, 398, 596
BseXI	GCAGC	7 cut(s)	120, 462, 466, 469, 916, 1083, 1192
BseYI	CCCAGC	4 cut(s)	347, 558, 1040, 1426
BsgI	GTGCAG	2 cut(s)	347, 1295
Bsh1236I	CGCG	5 cut(s)	210, 266, 275, 675, 1230
Bsh1285I	CGRYCG	1 cut(s)	20
BshNI	GGYRCC	1 cut(s)	1609
BshTI	ACCGGT	1 cut(s)	1076
BshVI	ATCGAT	1 cut(s)	318
BsiEI	CGRYCG	1 cut(s)	20
BsiHKAI	GWGCWC	2 cut(s)	390, 898
BsiSI	CCGG	6 cut(s)	418, 438, 852, 1077, 1176, 1416
BslFI	GGGAC	2 cut(s)	1288, 1558
BslI	CCNNNNNNNGG	4 cut(s)	54, 725, 1041, 1156
BsmAI	GTCTC	5 cut(s)	708, 756, 1108, 1399, 1753
BsmBI	CGTCTC	1 cut(s)	1399
BsmFI	GGGAC	2 cut(s)	1288, 1558
Bso31I	GGTCTC	1 cut(s)	1108
Bsp1286I	GDGCHC	3 cut(s)	390, 898, 1360
Bsp1407I	TGTACA	1 cut(s)	1053
Bsp143I	GATC	8 cut(s)	17, 216, 258, 315, 690, 1257, 1616, 1733
Bsp68I	TCGCGA	1 cut(s)	266
BspCNI	CTCAG	4 cut(s)	867, 1444, 1706, 1763
BspDI	ATCGAT	1 cut(s)	318
BspFNI	CGCG	5 cut(s)	210, 266, 275, 675, 1230
BspLI	GGNNCC	9 cut(s)	109, 436, 610, 859, 1357, 1470, 1546, 1547, 1611
BspMAI	CTGCAG	2 cut(s)	452, 1073
BspMI	ACCTGC	2 cut(s)	43, 1076
BspPI	GGATC	3 cut(s)	323, 698, 1624
BspT107I	GGYRCC	1 cut(s)	1609
BspTNI	GGTCTC	1 cut(s)	1108
BsrBI	CCGCTC	1 cut(s)	235
BsrDI	GCAATG	3 cut(s)	358, 1035, 1353
BsrFI	RCCGGY	2 cut(s)	1076, 1415
BsrGI	TGTACA	1 cut(s)	1053
BsrI	ACTGG	3 cut(s)	864, 1220, 1342
BssAI	RCCGGY	2 cut(s)	1076, 1415
BssECI	CCNNGG	7 cut(s)	42, 111, 597, 826, 981, 1228, 1360
BssMI	GATC	8 cut(s)	17, 216, 258, 315, 690, 1257, 1616, 1733
BssNI	GRCGYC	6 cut(s)	6, 123, 230, 357, 414, 1301
BssSI	CACGAG	2 cut(s)	83, 389
BssT1I	CCWWGG	2 cut(s)	42, 111
Bst2BI	CACGAG	2 cut(s)	83, 389
Bst2UI	CCWGG	1 cut(s)	1614
Bst4CI	ACNGT	4 cut(s)	827, 985, 1534, 1642
Bst6I	CTCTTC	1 cut(s)	176
BstACI	GRCGYC	6 cut(s)	6, 123, 230, 357, 414, 1301
BstAPI	GCANNNNNTGC	1 cut(s)	1185
BstAUI	TGTACA	1 cut(s)	1053
BstBAI	YACGTR	1 cut(s)	1498
BstC8I	GCNNGC	4 cut(s)	817, 1203, 1313, 1384
BstDEI	CTNAG	5 cut(s)	875, 921, 1431, 1693, 1750
BstDSI	CCRYGG	4 cut(s)	597, 826, 981, 1228
BstF5I	GGATG	5 cut(s)	612, 891, 1098, 1165, 1760
BstFNI	CGCG	5 cut(s)	210, 266, 275, 675, 1230
BstH2I	RGCGCY	2 cut(s)	397, 683
BstHHI	GCGC	4 cut(s)	210, 396, 675, 682
BstKTI	GATC	8 cut(s)	20, 219, 261, 318, 693, 1260, 1619, 1736
BstMAI	GTCTC	5 cut(s)	708, 756, 1108, 1399, 1753
BstMBI	GATC	8 cut(s)	17, 216, 258, 315, 690, 1257, 1616, 1733
BstMCI	CGRYCG	1 cut(s)	20
BstNI	CCWGG	1 cut(s)	1614
BstNSI	RCATGY	2 cut(s)	1324, 1382
BstSCI	CCNGG	2 cut(s)	416, 1612
BstSFI	CTRYAG	2 cut(s)	448, 1069
BstUI	CGCG	5 cut(s)	210, 266, 275, 675, 1230
BstV1I	GCAGC	7 cut(s)	120, 462, 466, 469, 916, 1083, 1192
BstV2I	GAAGAC	1 cut(s)	151
BstXI	CCANNNNNNTGG	1 cut(s)	1645
Bsu15I	ATCGAT	1 cut(s)	318
BsuI	GTATCC	1 cut(s)	22
BsuTUI	ATCGAT	1 cut(s)	318
BtgI	CCRYGG	4 cut(s)	597, 826, 981, 1228
BtgZI	GCGATG	1 cut(s)	1393
BtrI	CACGTC	1 cut(s)	241
BtsCI	GGATG	5 cut(s)	612, 891, 1098, 1165, 1760
BtsI	GCAGTG	1 cut(s)	1450
BtsIMutI	CAGTG	3 cut(s)	871, 1450, 1638
BtuMI	TCGCGA	1 cut(s)	266
BveI	ACCTGC	2 cut(s)	43, 1076
Cac8I	GCNNGC	4 cut(s)	817, 1203, 1313, 1384
CfoI	GCGC	4 cut(s)	210, 396, 675, 682
Cfr10I	RCCGGY	2 cut(s)	1076, 1415
Cfr13I	GGNCC	8 cut(s)	56, 79, 108, 222, 363, 435, 1489, 1545
Cfr42I	CCGCGG	1 cut(s)	1231
ClaI	ATCGAT	1 cut(s)	318
CseI	GACGC	4 cut(s)	131, 238, 422, 1290
Csp6I	GTAC	4 cut(s)	142, 782, 1054, 1530
CspAI	ACCGGT	1 cut(s)	1076
CviAII	CATG	9 cut(s)	1057, 1099, 1156, 1321, 1379, 1462, 1510, 1681, 1720
CviQI	GTAC	4 cut(s)	142, 782, 1054, 1530
DdeI	CTNAG	5 cut(s)	875, 921, 1431, 1693, 1750
DpnI	GATC	8 cut(s)	19, 218, 260, 317, 692, 1259, 1618, 1735
DpnII	GATC	8 cut(s)	17, 216, 258, 315, 690, 1257, 1616, 1733
DraIII	CACNNNGTG	2 cut(s)	244, 902
DrdI	GACNNNNNNGTC	1 cut(s)	98
DriI	GACNNNNNGTC	1 cut(s)	976
DseDI	GACNNNNNNGTC	1 cut(s)	98
EaeI	YGGCCR	3 cut(s)	789, 1417, 1635
Eam1104I	CTCTTC	1 cut(s)	176
Eam1105I	GACNNNNNGTC	1 cut(s)	976
EarI	CTCTTC	1 cut(s)	176
EciI	GGCGGA	3 cut(s)	332, 870, 1387
Eco130I	CCWWGG	2 cut(s)	42, 111
Eco147I	AGGCCT	1 cut(s)	116
Eco24I	GRGCYC	1 cut(s)	1360
Eco31I	GGTCTC	1 cut(s)	1108
Eco47I	GGWCC	4 cut(s)	79, 222, 1489, 1545
Eco47III	AGCGCT	1 cut(s)	395
Eco57I	CTGAAG	2 cut(s)	668, 1001
Eco72I	CACGTG	1 cut(s)	1498
EcoO109I	RGGNCCY	3 cut(s)	222, 1489, 1545
EcoRI	GAATTC	1 cut(s)	740
EcoRII	CCWGG	1 cut(s)	1612
EcoT14I	CCWWGG	2 cut(s)	42, 111
EcoT38I	GRGCYC	1 cut(s)	1360
ErhI	CCWWGG	2 cut(s)	42, 111
Esp3I	CGTCTC	1 cut(s)	1399
FaeI	CATG	9 cut(s)	1060, 1102, 1159, 1324, 1382, 1465, 1513, 1684, 1723
FalI	AAGNNNNNCTT	2 cut(s)	932, 964
FaqI	GGGAC	2 cut(s)	1288, 1558
FatI	CATG	9 cut(s)	1056, 1098, 1155, 1320, 1378, 1461, 1509, 1680, 1719
FauI	CCCGC	2 cut(s)	598, 1210
FbaI	TGATCA	1 cut(s)	1257
FblI	GTMKAC	1 cut(s)	186
FokI	GGATG	5 cut(s)	599, 898, 1105, 1172, 1747
FriOI	GRGCYC	1 cut(s)	1360
GlaI	GCGC	4 cut(s)	209, 395, 674, 681
GsaI	CCCAGC	4 cut(s)	351, 562, 1044, 1430
GsuI	CTGGAG	4 cut(s)	650, 1121, 1374, 1686
HaeII	RGCGCY	2 cut(s)	397, 683
HapII	CCGG	6 cut(s)	418, 438, 852, 1077, 1176, 1416
HgaI	GACGC	4 cut(s)	131, 238, 422, 1290
HhaI	GCGC	4 cut(s)	210, 396, 675, 682
Hin1I	GRCGYC	6 cut(s)	6, 123, 230, 357, 414, 1301
Hin1II	CATG	9 cut(s)	1060, 1102, 1159, 1324, 1382, 1465, 1513, 1684, 1723
Hin6I	GCGC	4 cut(s)	208, 394, 673, 680
HinP1I	GCGC	4 cut(s)	208, 394, 673, 680
HincII	GTYRAC	1 cut(s)	567
HindII	GTYRAC	1 cut(s)	567
HinfI	GANTC	6 cut(s)	306, 441, 632, 1005, 1062, 1368
HpaII	CCGG	6 cut(s)	418, 438, 852, 1077, 1176, 1416
HphI	GGTGA	7 cut(s)	79, 544, 1057, 1091, 1222, 1277, 1498
Hpy166II	GTNNAC	6 cut(s)	79, 187, 247, 567, 981, 1213
Hpy188III	TCNNGA	9 cut(s)	220, 244, 265, 313, 629, 753, 775, 1138, 1327
Hpy8I	GTNNAC	6 cut(s)	79, 187, 247, 567, 981, 1213
HpyCH4III	ACNGT	4 cut(s)	827, 985, 1534, 1642
HpyCH4IV	ACGT	6 cut(s)	6, 93, 240, 357, 1122, 1497
HpyF3I	CTNAG	5 cut(s)	875, 921, 1431, 1693, 1750
HpySE526I	ACGT	6 cut(s)	6, 93, 240, 357, 1122, 1497
Hsp92I	GRCGYC	6 cut(s)	6, 123, 230, 357, 414, 1301
Hsp92II	CATG	9 cut(s)	1060, 1102, 1159, 1324, 1382, 1465, 1513, 1684, 1723
HspAI	GCGC	4 cut(s)	208, 394, 673, 680
KflI	GGGWCCC	1 cut(s)	1545
Ksp22I	TGATCA	1 cut(s)	1257
KspI	CCGCGG	1 cut(s)	1231
Kzo9I	GATC	8 cut(s)	17, 216, 258, 315, 690, 1257, 1616, 1733
LmnI	GCTCC	4 cut(s)	240, 385, 857, 1355
Lsp1109I	GCAGC	7 cut(s)	120, 462, 466, 469, 916, 1083, 1192
LweI	GCATC	2 cut(s)	1072, 1565
MaeII	ACGT	6 cut(s)	6, 93, 240, 357, 1122, 1497
MaeIII	GTNAC	2 cut(s)	1504, 1715
MalI	GATC	8 cut(s)	19, 218, 260, 317, 692, 1259, 1618, 1735
MbiI	CCGCTC	1 cut(s)	235
MboI	GATC	8 cut(s)	17, 216, 258, 315, 690, 1257, 1616, 1733
MboII	GAAGA	9 cut(s)	151, 154, 163, 166, 226, 301, 587, 965, 1057
MfeI	CAATTG	1 cut(s)	1518
MhlI	GDGCHC	3 cut(s)	390, 898, 1360
MlsI	TGGCCA	2 cut(s)	791, 1637
MluCI	AATT	7 cut(s)	456, 465, 740, 964, 1023, 1170, 1518
MluNI	TGGCCA	2 cut(s)	791, 1637
MlyI	GAGTC	2 cut(s)	435, 641
MmeI	TCCRAC	2 cut(s)	144, 1516
Mox20I	TGGCCA	2 cut(s)	791, 1637
MroXI	GAANNNNTTC	1 cut(s)	1145
MscI	TGGCCA	2 cut(s)	791, 1637
MseI	TTAA	3 cut(s)	1026, 1317, 1768
MslI	CAYNNNNRTG	4 cut(s)	866, 1160, 1460, 1643
Msp20I	TGGCCA	2 cut(s)	791, 1637
MspA1I	CMGCKG	5 cut(s)	133, 1205, 1230, 1697, 1754
MspI	CCGG	6 cut(s)	418, 438, 852, 1077, 1176, 1416
MspR9I	CCNGG	2 cut(s)	418, 1614
MunI	CAATTG	1 cut(s)	1518
MvaI	CCWGG	1 cut(s)	1614
MvnI	CGCG	5 cut(s)	210, 266, 275, 675, 1230
NciI	CCSGG	1 cut(s)	418
NdeII	GATC	8 cut(s)	17, 216, 258, 315, 690, 1257, 1616, 1733
NlaIII	CATG	9 cut(s)	1060, 1102, 1159, 1324, 1382, 1465, 1513, 1684, 1723
NlaIV	GGNNCC	9 cut(s)	109, 436, 610, 859, 1357, 1470, 1546, 1547, 1611
NmeAIII	GCCGAG	1 cut(s)	958
NmuCI	GTSAC	2 cut(s)	1504, 1715
NruI	TCGCGA	1 cut(s)	266
NspI	RCATGY	2 cut(s)	1324, 1382
OliI	CACNNNNGTG	2 cut(s)	866, 1643
PaqCI	CACCTGC	2 cut(s)	43, 1076
PceI	AGGCCT	1 cut(s)	116
PcsI	WCGNNNNNNNCGW	2 cut(s)	90, 99
PdmI	GAANNNNTTC	1 cut(s)	1145
PfeI	GAWTC	4 cut(s)	306, 1005, 1062, 1368
PinAI	ACCGGT	1 cut(s)	1076
Ple19I	CGATCG	1 cut(s)	20
PleI	GAGTC	2 cut(s)	435, 640
PmaCI	CACGTG	1 cut(s)	1498
PmlI	CACGTG	1 cut(s)	1498
PpsI	GAGTC	2 cut(s)	435, 640
Ppu21I	YACGTR	1 cut(s)	1498
PpuMI	RGGWCCY	3 cut(s)	222, 1489, 1545
Psp5II	RGGWCCY	3 cut(s)	222, 1489, 1545
Psp6I	CCWGG	1 cut(s)	1612
PspCI	CACGTG	1 cut(s)	1498
PspFI	CCCAGC	4 cut(s)	347, 558, 1040, 1426
PspGI	CCWGG	1 cut(s)	1612
PspN4I	GGNNCC	9 cut(s)	109, 436, 610, 859, 1357, 1470, 1546, 1547, 1611
PspPI	GGNCC	8 cut(s)	56, 79, 108, 222, 363, 435, 1489, 1545
PspPPI	RGGWCCY	3 cut(s)	222, 1489, 1545
PstI	CTGCAG	2 cut(s)	452, 1073
PvuI	CGATCG	1 cut(s)	20
PvuII	CAGCTG	2 cut(s)	1697, 1754
RruI	TCGCGA	1 cut(s)	266
RsaI	GTAC	4 cut(s)	143, 783, 1055, 1531
RsaNI	GTAC	4 cut(s)	142, 782, 1054, 1530
RseI	CAYNNNNRTG	4 cut(s)	866, 1160, 1460, 1643
SacII	CCGCGG	1 cut(s)	1231
SaqAI	TTAA	3 cut(s)	1026, 1317, 1768
Sau3AI	GATC	8 cut(s)	17, 216, 258, 315, 690, 1257, 1616, 1733
Sau96I	GGNCC	8 cut(s)	56, 79, 108, 222, 363, 435, 1489, 1545
SchI	GAGTC	2 cut(s)	435, 641
ScrFI	CCNGG	2 cut(s)	418, 1614
SduI	GDGCHC	3 cut(s)	390, 898, 1360
SfaNI	GCATC	2 cut(s)	1072, 1565
SfcI	CTRYAG	2 cut(s)	448, 1069
Sfr303I	CCGCGG	1 cut(s)	1231
SgrAI	CRCCGGYG	1 cut(s)	1076
SgrBI	CCGCGG	1 cut(s)	1231
SinI	GGWCC	4 cut(s)	79, 222, 1489, 1545
SmiMI	CAYNNNNRTG	4 cut(s)	866, 1160, 1460, 1643
SmlI	CTYRAG	1 cut(s)	1325
SmoI	CTYRAG	1 cut(s)	1325
Sse9I	AATT	7 cut(s)	456, 465, 740, 964, 1023, 1170, 1518
SseBI	AGGCCT	1 cut(s)	116
StuI	AGGCCT	1 cut(s)	116
StyD4I	CCNGG	2 cut(s)	416, 1612
StyI	CCWWGG	2 cut(s)	42, 111
TaaI	ACNGT	4 cut(s)	827, 985, 1534, 1642
TaiI	ACGT	6 cut(s)	9, 96, 243, 360, 1125, 1500
TaqI	TCGA	6 cut(s)	20, 227, 318, 444, 732, 754
TasI	AATT	7 cut(s)	456, 465, 740, 964, 1023, 1170, 1518
TatI	WGTACW	1 cut(s)	1053
TauI	GCSGC	7 cut(s)	133, 235, 368, 488, 662, 665, 1230
TfiI	GAWTC	4 cut(s)	306, 1005, 1062, 1368
Tru1I	TTAA	3 cut(s)	1026, 1317, 1768
Tru9I	TTAA	3 cut(s)	1026, 1317, 1768
TscAI	CASTG	3 cut(s)	871, 1457, 1645
TseFI	GTSAC	2 cut(s)	1504, 1715
TseI	GCWGC	7 cut(s)	133, 450, 479, 482, 929, 1071, 1205
Tsp45I	GTSAC	2 cut(s)	1504, 1715
TspDTI	ATGAA	4 cut(s)	77, 280, 1109, 1736
TspGWI	ACGGA	3 cut(s)	169, 584, 586
TspRI	CASTG	3 cut(s)	871, 1457, 1645
VpaK11BI	GGWCC	4 cut(s)	79, 222, 1489, 1545
XapI	RAATTY	2 cut(s)	740, 1170
XceI	RCATGY	2 cut(s)	1324, 1382
XmiI	GTMKAC	1 cut(s)	186
XmnI	GAANNNNTTC	1 cut(s)	1145
ZraI	GACGTC	2 cut(s)	7, 358

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.