MD05G1256300.v1.1

Regulatory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000570
Physical Location & Seq
Forward (+)
38744751 .. 38748169
3419 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1256300.v1.1.491

Sequence Viewer

Length: 1764 bp
ATGGCTTATTCAGCTGAACCATCATCCTCTTTGAGCTTTACTTCCTCATCCCCTTTGTCAAATGGTTCGATATGCCACAACGTATCGTGCTCGGGCTCCGAATCGGTGCCAAATCTTGAAGTAATCAGTTTGTCCAAGCTTAGTTCCAGTTTGGAGCAGCTGTTGGTTGATCCTGGATGCGATTTTAGTGATGCTGATATCATCGTTGAGGGGATTCCTGTTGGTGTTCACCGATGTATATTGGCTTCGAGGAGTAGGTTTTTTCGCGAGCTATTCAAGCGAGAAAAGGGGTCTTCTGGGAAGGGAGGCAGGCCAAAGTACTCTATGAGCGATTTGCTGCCTTACGGGGATGTTGGATACGAAGCCTTCTTGGTTTTCTTAAGCTATGTGTATACCGGAAAGCTTAAGCCTTCTCCAATGGAGGTGTCAACGTGCGTTCACAATGTATGTGCCCATGACGCTTGTAGACCTGCTATTAATTTCGTTGTGGAATTGATGTACGCCGCTTCCGTTTTCCAAATGCCCGATCTGGTTTCGATATTCGAGCGGCGCCTTCTTAATTTTGTTGGGAAAGCTCTGCCGGACAATGTTATCCCGATTCTCGTGGTTGCTTTCCATTGCCAGTTGAATCAGCTCATCGCTCAGTGTATAGAGAGAGTGGCTCGATCGGATATTGATGATATCTCGATTGAGAAGGGACTTCCTGATGAGGTTGTGAAGAAAATCAAAGTTCTTCGCCGCAAACCTCAGGAGGACTGTGTCTCAAACTTGCCGCCTGTGGATCCCTTGCATGAAAAGAGAATCCGGAGAATACATAAGGCTTTGGACTCGGATGATGTTGAACTTGTGAAACTTCTTTTGACGGAGTCTAATATAACCCTAGACGAAGCAAATGCTCTTCATTATGCTGCAGCTTACTGCGATCCTAAGGTTGTGACTGAAGTGCTTGATCTGGGTCTCGCTGATGTTAACCTCCGGAATTCTCGTGGTTATACAGTGCTTCACATTGCTGTCATGCGCAAAGAGCCATCAATTATCGTATTGCTTCTCACTAAAGGAGCTCGTGCGTCGGAGCTAACATCAGATGGTCAGAGTGCTGTTAGTATTTGCCGGAGGTTGACAAGACCAAAGGATTACCATTCAAAAACAGAGCAGGGGCAAGAAGCAAACAAAGACCGAATATGCATTGATGTTCTAGAGAGGGAAATGCGGCGGAATCCCATGGCTGGAGATACATCTATATCTTCCCAAATAATGCCTGATGATCTGCACATGGAGTTGCTGAACCTGGAGAACAGAGTGGCAATTGCGCGATTGTTATTTCCTGCGGAGGCCAAGCTAGCCATGGTCGTTGCCCATACAGAGGCAACGCCCGAGTTTCCTAAGCCATCGTCATCGAAAGGCTCAAGTGGGAATCTGATGGAGGTTGATTTAAATGAGACCCCCACAATGCAGAACAAAAGACTCCACTCTAGGTTGGAAGCCCTTATGAAAACCGTCCGTTTGGGTAGATGCTATTTCCCTCACTGCTCGGAAGTCCTGGATAAGTTCATCGTCGACGACCTTCCTGAATTGTTTTTCCTCGAGTCCGGCTCCTCTGATGAGCAGAAGGTAAAGAGGAGGCGTTTCATGGAGCTGAAGGAGGAAGTACAAAAGGCATTTACCAAAGACAAGGCTGAGTTGAGCCTCTCCGGGTTGTCTTCATCATCCTCCTCGTCATCTCCCAGAAAATTTGGCACAAATCAGAAGGTTAGGAAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008144 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009814 GO:0009816 GO:0009817 GO:0009966 GO:0009987 GO:0010498 GO:0010646 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0023051 GO:0030162 GO:0030163 GO:0031406 GO:0031461 GO:0031625 GO:0032991 GO:0033293 GO:0035821 GO:0036094 GO:0042562 GO:0042742 GO:0042802 GO:0043161 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044003 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051246 GO:0051603 GO:0051701 GO:0051704 GO:0051707 GO:0051817 GO:0052031 GO:0052173 GO:0052200 GO:0052255 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052558 GO:0052559 GO:0052564 GO:0052572 GO:0060255 GO:0065007 GO:0071704 GO:0075136 GO:0080090 GO:0080185 GO:0097159 GO:0098542 GO:1901149 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234 GO:2000022 GO:2000031 GO:2001023 GO:2001038
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 BTB DUF3420 NPR1_like_C

Protein Analysis

588

Amino Acids

65.23

Weight (kDa)

6.72

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 61 - 183 7.8e-10 BTB/POZ domain
DUF3420 PF11900 224 - 272 2.4e-13 Domain of unknown function (DUF3420)
Ank_2 PF12796 272 - 355 6.5e-10 Ankyrin repeats (3 copies)
NPR1_like_C PF12313 371 - 573 1.1e-87 NPR1/NIM1 like defence protein C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000570)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45110 AT5G45110
fragaria_vesca FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_6g38820 FvH4_6g38820 FvH4_6g38821 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830
malus_domestica MD05G1256300.v1.1 MD05G1256400.v1.1 MD09G1146600.v1.1 MD10G1236700.v1.1 MD10G1236800.v1.1 MD17G1133300.v1.1
prunus_persica Prupe.3G178800_v2.0.a1 Prupe.3G178800_v2.0.a1 Prupe.3G178800_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1
pyrus_communis pycom05g23540 pycom05g23550 pycom05g23560 pycom05g23570 pycom09g06690 pycom10g19800 pycom17g12780
rosa_chinensis RchiOBHm_Chr2g0152421 RchiOBHm_Chr2g0152431 RchiOBHm_Chr2g0152441 RchiOBHm_Chr5g0019531
rosa_laevigata RLG00000020632 RLG00000020633 RLG00000032453
rosa_multiflora Rmu_co8414461.1_g000001 Rmu_sc0002759.1_g000050 Rmu_sc0005366.1_g000015 Rmu_sc0005366.1_g000016 Rmu_sc0005366.1_g000017 Rmu_sc0005366.1_g000020 Rmu_sc0005366.1_g000022
rosa_roxburghii Rroxscaffold_1G00058530 Rroxscaffold_2G00095720 Rroxscaffold_2G00095730 Rroxscaffold_2G00095740
rosa_rugosa Rorug02G0432800 Rorug05G0051600
rosa_samantha Rh2AG494300 Rh2AG494400 Rh2AG494500 Rh2BG506600 Rh2BG506700 Rh2CG480600 Rh2CG480700 Rh2DG517700 Rh2DG517800 Rh2DG517900 Rh5AG142400 Rh5BG140800 Rh5CG152900 Rh5DG141500
rosa_wichuraiana Rw2G040670 Rw2G040680 Rw2G040690 Rw5G012600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1019
Acc36I ACCTGC 1 cut(s) 478
AccB1I GGYRCC 2 cut(s) 106, 549
AccBSI CCGCTC 1 cut(s) 547
AccI GTMKAC 3 cut(s) 392, 466, 1557
AccII CGCG 2 cut(s) 267, 1312
AccIII TCCGGA 2 cut(s) 804, 975
AciI CCGC 7 cut(s) 504, 547, 739, 773, 1210, 1213, 1328
AclWI GGATC 4 cut(s) 164, 776, 789, 917
AcsI RAATTY 2 cut(s) 979, 1730
AcuI CTGAAG 2 cut(s) 960, 1658
AcyI GRCGYC 1 cut(s) 550
AfaI GTAC 3 cut(s) 320, 500, 1650
AfiI CCNNNNNNNGG 2 cut(s) 1226, 1363
AflII CTTAAG 2 cut(s) 379, 404
AgsI TTSAA 5 cut(s) 119, 277, 628, 842, 1143
AjnI CCWGG 3 cut(s) 172, 1287, 1539
Alw21I GWGCWC 2 cut(s) 92, 1063
Alw26I GTCTC 3 cut(s) 766, 962, 1433
AlwI GGATC 4 cut(s) 164, 776, 789, 917
Ama87I CYCGRG 3 cut(s) 91, 1373, 1583
Aor13HI TCCGGA 2 cut(s) 804, 975
AoxI GGCC 2 cut(s) 311, 1332
ApeKI GCWGC 4 cut(s) 157, 337, 908, 911
ApoI RAATTY 2 cut(s) 979, 1730
AseI ATTAAT 1 cut(s) 477
AspLEI GCGC 3 cut(s) 552, 1020, 1312
AsuC2I CCSGG 1 cut(s) 1693
AsuHPI GGTGA 1 cut(s) 221
AsuNHI GCTAGC 1 cut(s) 1339
AvaI CYCGRG 3 cut(s) 91, 1373, 1583
AxyI CCTNAGG 2 cut(s) 747, 927
BaeGI GKGCMC 1 cut(s) 454
BamHI GGATCC 1 cut(s) 781
BanI GGYRCC 2 cut(s) 106, 549
BanII GRGCYC 2 cut(s) 98, 1063
BarI GAAGNNNNNNTAC 2 cut(s) 229, 261
BauI CACGAG 3 cut(s) 602, 984, 1062
BbsI GAAGAC 2 cut(s) 285, 1692
Bbv12I GWGCWC 2 cut(s) 92, 1063
BbvI GCAGC 4 cut(s) 169, 324, 895, 923
BccI CCATC 5 cut(s) 28, 1036, 1079, 1396, 1414
BcgI CGANNNNNNTGC 2 cut(s) 875, 909
BciT130I CCWGG 3 cut(s) 174, 1289, 1541
BciVI GTATCC 1 cut(s) 350
BcnI CCSGG 1 cut(s) 1693
BcoDI GTCTC 3 cut(s) 766, 962, 1433
BfaI CTAG 4 cut(s) 881, 1196, 1340, 1473
BfmI CTRYAG 1 cut(s) 909
BfoI RGCGCY 1 cut(s) 553
BfrI CTTAAG 2 cut(s) 379, 404
BfuAI ACCTGC 1 cut(s) 478
BfuI GTATCC 1 cut(s) 350
BisI GCNGC 9 cut(s) 158, 338, 504, 548, 739, 773, 909, 912, 1211
BlsI GCNGC 9 cut(s) 159, 339, 505, 549, 740, 774, 910, 913, 1212
BmcAI AGTACT 1 cut(s) 320
Bme1390I CCNGG 4 cut(s) 174, 1289, 1541, 1693
BmeT110I CYCGRG 3 cut(s) 91, 1373, 1583
BmiI GGNNCC 5 cut(s) 97, 108, 551, 783, 1594
BmrFI CCNGG 4 cut(s) 174, 1289, 1541, 1693
BmsI GCATC 3 cut(s) 167, 181, 1502
BmtI GCTAGC 1 cut(s) 1343
BpiI GAAGAC 2 cut(s) 285, 1692
BplI GAGNNNNNCTC 4 cut(s) 646, 678, 1577, 1609
BpmI CTGGAG 2 cut(s) 1248, 1310
Bpu10I CCTNAGC 1 cut(s) 1383
BpuEI CTTGAG 1 cut(s) 1390
BpuMI CCSGG 1 cut(s) 1693
BsaHI GRCGYC 1 cut(s) 550
BsaI GGTCTC 2 cut(s) 962, 1433
BsaJI CCNNGG 2 cut(s) 1221, 1344
BsaWI WCCGGW 3 cut(s) 395, 804, 975
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 2 cut(s) 1226, 1363
Bse1I ACTGG 2 cut(s) 147, 622
Bse21I CCTNAGG 2 cut(s) 747, 927
Bse3DI GCAATG 2 cut(s) 616, 1005
BseAI TCCGGA 2 cut(s) 804, 975
BseBI CCWGG 3 cut(s) 174, 1289, 1541
BseDI CCNNGG 2 cut(s) 1221, 1344
BseGI GGATG 6 cut(s) 23, 47, 182, 355, 838, 1706
BseLI CCNNNNNNNGG 2 cut(s) 1226, 1363
BseMI GCAATG 2 cut(s) 616, 1005
BseMII CTCAG 3 cut(s) 656, 761, 1668
BseNI ACTGG 2 cut(s) 147, 622
BseRI GAGGAG 4 cut(s) 265, 1585, 1633, 1702
BseSI GKGCMC 1 cut(s) 454
BseXI GCAGC 4 cut(s) 169, 324, 895, 923
BsgI GTGCAG 1 cut(s) 1253
Bsh1236I CGCG 2 cut(s) 267, 1312
Bsh1285I CGRYCG 1 cut(s) 668
BshFI GGCC 2 cut(s) 313, 1334
BshNI GGYRCC 2 cut(s) 106, 549
BsiEI CGRYCG 1 cut(s) 668
BsiHKAI GWGCWC 2 cut(s) 92, 1063
BsiHKCI CYCGRG 3 cut(s) 91, 1373, 1583
BsiSI CCGG 7 cut(s) 396, 581, 805, 976, 1111, 1590, 1692
BslFI GGGAC 1 cut(s) 711
BslI CCNNNNNNNGG 2 cut(s) 1226, 1363
BsmAI GTCTC 3 cut(s) 766, 962, 1433
BsmFI GGGAC 1 cut(s) 711
BsnI GGCC 2 cut(s) 313, 1334
Bso31I GGTCTC 2 cut(s) 962, 1433
BsoBI CYCGRG 3 cut(s) 91, 1373, 1583
Bsp1286I GDGCHC 4 cut(s) 92, 98, 454, 1063
Bsp13I TCCGGA 2 cut(s) 804, 975
Bsp143I GATC 7 cut(s) 169, 526, 665, 781, 922, 949, 1264
Bsp19I CCATGG 2 cut(s) 1221, 1344
Bsp68I TCGCGA 1 cut(s) 267
BspACI CCGC 7 cut(s) 504, 547, 739, 773, 1210, 1213, 1328
BspANI GGCC 2 cut(s) 313, 1334
BspCNI CTCAG 3 cut(s) 655, 760, 1669
BspEI TCCGGA 2 cut(s) 804, 975
BspFNI CGCG 2 cut(s) 267, 1312
BspLI GGNNCC 5 cut(s) 97, 108, 551, 783, 1594
BspMAI CTGCAG 1 cut(s) 913
BspMI ACCTGC 1 cut(s) 478
BspOI GCTAGC 1 cut(s) 1343
BspPI GGATC 4 cut(s) 164, 776, 789, 917
BspQI GCTCTTC 1 cut(s) 903
BspT107I GGYRCC 2 cut(s) 106, 549
BspTI CTTAAG 2 cut(s) 379, 404
BspTNI GGTCTC 2 cut(s) 962, 1433
BsrBI CCGCTC 1 cut(s) 547
BsrDI GCAATG 2 cut(s) 616, 1005
BsrI ACTGG 2 cut(s) 147, 622
BssECI CCNNGG 2 cut(s) 1221, 1344
BssMI GATC 7 cut(s) 169, 526, 665, 781, 922, 949, 1264
BssNAI GTATAC 1 cut(s) 393
BssNI GRCGYC 1 cut(s) 550
BssSI CACGAG 3 cut(s) 602, 984, 1062
BssT1I CCWWGG 2 cut(s) 1221, 1344
Bst1107I GTATAC 1 cut(s) 393
Bst2BI CACGAG 3 cut(s) 602, 984, 1062
Bst2UI CCWGG 3 cut(s) 174, 1289, 1541
Bst4CI ACNGT 3 cut(s) 758, 997, 1498
Bst6I CTCTTC 1 cut(s) 903
BstACI GRCGYC 1 cut(s) 550
BstAFI CTTAAG 2 cut(s) 379, 404
BstC8I GCNNGC 3 cut(s) 269, 311, 1341
BstDEI CTNAG 6 cut(s) 140, 642, 747, 927, 1383, 1677
BstDSI CCRYGG 2 cut(s) 1221, 1344
BstF5I GGATG 6 cut(s) 23, 47, 182, 355, 838, 1706
BstFNI CGCG 2 cut(s) 267, 1312
BstH2I RGCGCY 1 cut(s) 553
BstHHI GCGC 3 cut(s) 552, 1020, 1312
BstKTI GATC 7 cut(s) 172, 529, 668, 784, 925, 952, 1267
BstMAI GTCTC 3 cut(s) 766, 962, 1433
BstMBI GATC 7 cut(s) 169, 526, 665, 781, 922, 949, 1264
BstMCI CGRYCG 1 cut(s) 668
BstMWI GCNNNNNNNGC 5 cut(s) 11, 277, 458, 1024, 1340
BstNI CCWGG 3 cut(s) 174, 1289, 1541
BstSCI CCNGG 4 cut(s) 172, 1287, 1539, 1691
BstSFI CTRYAG 1 cut(s) 909
BstSLI GKGCMC 1 cut(s) 454
BstUI CGCG 2 cut(s) 267, 1312
BstV1I GCAGC 4 cut(s) 169, 324, 895, 923
BstV2I GAAGAC 2 cut(s) 285, 1692
BstX2I RGATCY 1 cut(s) 781
BstYI RGATCY 1 cut(s) 781
BstZ17I GTATAC 1 cut(s) 393
Bsu36I CCTNAGG 2 cut(s) 747, 927
BsuI GTATCC 1 cut(s) 350
BsuRI GGCC 2 cut(s) 313, 1334
BtgI CCRYGG 2 cut(s) 1221, 1344
BtgZI GCGATG 1 cut(s) 622
BtsCI GGATG 6 cut(s) 23, 47, 182, 355, 838, 1706
BtsI GCAGTG 1 cut(s) 1525
BtsIMutI CAGTG 3 cut(s) 650, 1002, 1525
BtuMI TCGCGA 1 cut(s) 267
BveI ACCTGC 1 cut(s) 478
Cac8I GCNNGC 3 cut(s) 269, 311, 1341
CfoI GCGC 3 cut(s) 552, 1020, 1312
CseI GACGC 2 cut(s) 467, 1056
Csp6I GTAC 3 cut(s) 319, 499, 1649
CviAII CATG 7 cut(s) 455, 791, 1015, 1222, 1273, 1345, 1630
CviQI GTAC 3 cut(s) 319, 499, 1649
DdeI CTNAG 6 cut(s) 140, 642, 747, 927, 1383, 1677
DinI GGCGCC 1 cut(s) 551
DpnI GATC 7 cut(s) 171, 528, 667, 783, 924, 951, 1266
DpnII GATC 7 cut(s) 169, 526, 665, 781, 922, 949, 1264
DraI TTTAAA 1 cut(s) 1434
Eam1104I CTCTTC 1 cut(s) 903
EarI CTCTTC 1 cut(s) 903
EciI GGCGGA 1 cut(s) 1228
Ecl136II GAGCTC 1 cut(s) 1061
Eco130I CCWWGG 2 cut(s) 1221, 1344
Eco24I GRGCYC 2 cut(s) 98, 1063
Eco31I GGTCTC 2 cut(s) 962, 1433
Eco32I GATATC 2 cut(s) 199, 682
Eco53kI GAGCTC 1 cut(s) 1061
Eco57I CTGAAG 2 cut(s) 960, 1658
Eco81I CCTNAGG 2 cut(s) 747, 927
Eco88I CYCGRG 3 cut(s) 91, 1373, 1583
EcoICRI GAGCTC 1 cut(s) 1061
EcoRI GAATTC 1 cut(s) 979
EcoRII CCWGG 3 cut(s) 172, 1287, 1539
EcoRV GATATC 2 cut(s) 199, 682
EcoT14I CCWWGG 2 cut(s) 1221, 1344
EcoT22I ATGCAT 1 cut(s) 1187
EcoT38I GRGCYC 2 cut(s) 98, 1063
EgeI GGCGCC 1 cut(s) 551
EheI GGCGCC 1 cut(s) 551
ErhI CCWWGG 2 cut(s) 1221, 1344
FaeI CATG 7 cut(s) 458, 794, 1018, 1225, 1276, 1348, 1633
FaqI GGGAC 1 cut(s) 711
FatI CATG 7 cut(s) 454, 790, 1014, 1221, 1272, 1344, 1629
FblI GTMKAC 3 cut(s) 392, 466, 1557
Fnu4HI GCNGC 9 cut(s) 158, 338, 504, 548, 739, 773, 909, 912, 1211
FokI GGATG 6 cut(s) 10, 34, 189, 362, 845, 1693
FriOI GRGCYC 2 cut(s) 98, 1063
Fsp4HI GCNGC 9 cut(s) 158, 338, 504, 548, 739, 773, 909, 912, 1211
FspBI CTAG 4 cut(s) 881, 1196, 1340, 1473
FspI TGCGCA 1 cut(s) 1019
GlaI GCGC 3 cut(s) 551, 1019, 1311
GluI GCNGC 9 cut(s) 158, 338, 504, 548, 739, 773, 909, 912, 1211
GsuI CTGGAG 2 cut(s) 1248, 1310
HaeII RGCGCY 1 cut(s) 553
HaeIII GGCC 2 cut(s) 313, 1334
HapII CCGG 7 cut(s) 396, 581, 805, 976, 1111, 1590, 1692
HgaI GACGC 2 cut(s) 467, 1056
HhaI GCGC 3 cut(s) 552, 1020, 1312
Hin1I GRCGYC 1 cut(s) 550
Hin1II CATG 7 cut(s) 458, 794, 1018, 1225, 1276, 1348, 1633
Hin6I GCGC 3 cut(s) 550, 1018, 1310
HinP1I GCGC 3 cut(s) 550, 1018, 1310
HincII GTYRAC 4 cut(s) 429, 970, 1119, 1558
HindII GTYRAC 4 cut(s) 429, 970, 1119, 1558
HindIII AAGCTT 2 cut(s) 137, 401
HpaI GTTAAC 1 cut(s) 970
HpaII CCGG 7 cut(s) 396, 581, 805, 976, 1111, 1590, 1692
HphI GGTGA 1 cut(s) 221
Hpy166II GTNNAC 8 cut(s) 229, 393, 429, 439, 467, 970, 1119, 1558
Hpy8I GTNNAC 8 cut(s) 229, 393, 429, 439, 467, 970, 1119, 1558
Hpy99I CGWCG 3 cut(s) 1072, 1559, 1562
HpyAV CCTTC 9 cut(s) 295, 376, 420, 563, 688, 1574, 1603, 1633, 1741
HpyCH4III ACNGT 3 cut(s) 758, 997, 1498
HpyCH4IV ACGT 2 cut(s) 81, 431
HpyCH4V TGCA 5 cut(s) 790, 911, 1185, 1270, 1453
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 277, 458, 1024, 1340
HpyF3I CTNAG 6 cut(s) 140, 642, 747, 927, 1383, 1677
HpySE526I ACGT 2 cut(s) 81, 431
Hsp92I GRCGYC 1 cut(s) 550
Hsp92II CATG 7 cut(s) 458, 794, 1018, 1225, 1276, 1348, 1633
HspAI GCGC 3 cut(s) 550, 1018, 1310
KasI GGCGCC 1 cut(s) 549
Kpn2I TCCGGA 2 cut(s) 804, 975
KspAI GTTAAC 1 cut(s) 970
Kzo9I GATC 7 cut(s) 169, 526, 665, 781, 922, 949, 1264
LguI GCTCTTC 1 cut(s) 903
LmnI GCTCC 6 cut(s) 101, 154, 1058, 1072, 1598, 1633
Lsp1109I GCAGC 4 cut(s) 169, 324, 895, 923
LweI GCATC 3 cut(s) 167, 181, 1502
MaeI CTAG 4 cut(s) 881, 1196, 1340, 1473
MaeII ACGT 2 cut(s) 81, 431
MaeIII GTNAC 1 cut(s) 934
MalI GATC 7 cut(s) 171, 528, 667, 783, 924, 951, 1266
MbiI CCGCTC 1 cut(s) 547
MboI GATC 7 cut(s) 169, 526, 665, 781, 922, 949, 1264
MboII GAAGA 6 cut(s) 285, 725, 730, 890, 1236, 1692
MfeI CAATTG 1 cut(s) 1305
MflI RGATCY 1 cut(s) 781
MhlI GDGCHC 4 cut(s) 92, 98, 454, 1063
MluCI AATT 9 cut(s) 478, 491, 559, 979, 1032, 1305, 1571, 1730, 1757
Mly113I GGCGCC 1 cut(s) 550
MlyI GAGTC 4 cut(s) 821, 875, 1458, 1595
MmeI TCCRAC 3 cut(s) 334, 1050, 1458
Mph1103I ATGCAT 1 cut(s) 1187
MroI TCCGGA 2 cut(s) 804, 975
MseI TTAA 6 cut(s) 380, 405, 477, 558, 969, 1433
MspA1I CMGCKG 2 cut(s) 14, 160
MspCI CTTAAG 2 cut(s) 379, 404
MspI CCGG 7 cut(s) 396, 581, 805, 976, 1111, 1590, 1692
MspR9I CCNGG 4 cut(s) 174, 1289, 1541, 1693
MunI CAATTG 1 cut(s) 1305
MvaI CCWGG 3 cut(s) 174, 1289, 1541
MvnI CGCG 2 cut(s) 267, 1312
MwoI GCNNNNNNNGC 5 cut(s) 11, 277, 458, 1024, 1340
NarI GGCGCC 1 cut(s) 550
NciI CCSGG 1 cut(s) 1693
NcoI CCATGG 2 cut(s) 1221, 1344
NdeII GATC 7 cut(s) 169, 526, 665, 781, 922, 949, 1264
NheI GCTAGC 1 cut(s) 1339
NlaIII CATG 7 cut(s) 458, 794, 1018, 1225, 1276, 1348, 1633
NlaIV GGNNCC 5 cut(s) 97, 108, 551, 783, 1594
NmuCI GTSAC 1 cut(s) 934
NruI TCGCGA 1 cut(s) 267
NsbI TGCGCA 1 cut(s) 1019
NsiI ATGCAT 1 cut(s) 1187
PaeR7I CTCGAG 1 cut(s) 1583
PciSI GCTCTTC 1 cut(s) 903
PcsI WCGNNNNNNNCGW 1 cut(s) 507
PfeI GAWTC 7 cut(s) 101, 214, 598, 628, 801, 1216, 1414
PflFI GACNNNGTC 2 cut(s) 758, 865
PfoI TCCNGGA 2 cut(s) 172, 1539
PkrI GCNGC 9 cut(s) 159, 339, 505, 549, 740, 774, 910, 913, 1212
Ple19I CGATCG 1 cut(s) 668
PleI GAGTC 4 cut(s) 821, 874, 1458, 1594
PluTI GGCGCC 1 cut(s) 553
PpsI GAGTC 4 cut(s) 821, 874, 1458, 1594
PshBI ATTAAT 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 1063
Psp6I CCWGG 3 cut(s) 172, 1287, 1539
PspGI CCWGG 3 cut(s) 172, 1287, 1539
PspN4I GGNNCC 5 cut(s) 97, 108, 551, 783, 1594
PspXI VCTCGAGB 1 cut(s) 1583
PstI CTGCAG 1 cut(s) 913
PsuI RGATCY 1 cut(s) 781
PsyI GACNNNGTC 2 cut(s) 758, 865
PvuI CGATCG 1 cut(s) 668
PvuII CAGCTG 2 cut(s) 14, 160
RruI TCGCGA 1 cut(s) 267
RsaI GTAC 3 cut(s) 320, 500, 1650
RsaNI GTAC 3 cut(s) 319, 499, 1649
SacI GAGCTC 1 cut(s) 1063
SalI GTCGAC 1 cut(s) 1556
SapI GCTCTTC 1 cut(s) 903
SaqAI TTAA 6 cut(s) 380, 405, 477, 558, 969, 1433
SatI GCNGC 9 cut(s) 158, 338, 504, 548, 739, 773, 909, 912, 1211
Sau3AI GATC 7 cut(s) 169, 526, 665, 781, 922, 949, 1264
ScaI AGTACT 1 cut(s) 320
SchI GAGTC 4 cut(s) 821, 875, 1458, 1595
ScrFI CCNGG 4 cut(s) 174, 1289, 1541, 1693
SduI GDGCHC 4 cut(s) 92, 98, 454, 1063
SfaNI GCATC 3 cut(s) 167, 181, 1502
SfcI CTRYAG 1 cut(s) 909
SfoI GGCGCC 1 cut(s) 551
Sfr274I CTCGAG 1 cut(s) 1583
SgrDI CGTCGACG 1 cut(s) 1556
SlaI CTCGAG 1 cut(s) 1583
SmiI ATTTAAAT 1 cut(s) 1434
SmlI CTYRAG 4 cut(s) 379, 404, 1405, 1583
SmoI CTYRAG 4 cut(s) 379, 404, 1405, 1583
Sse9I AATT 9 cut(s) 478, 491, 559, 979, 1032, 1305, 1571, 1730, 1757
SsiI CCGC 7 cut(s) 504, 547, 739, 773, 1210, 1213, 1328
SspDI GGCGCC 1 cut(s) 549
SspMI CTAG 4 cut(s) 881, 1196, 1340, 1473
SstI GAGCTC 1 cut(s) 1063
StyD4I CCNGG 4 cut(s) 172, 1287, 1539, 1691
StyI CCWWGG 2 cut(s) 1221, 1344
SwaI ATTTAAAT 1 cut(s) 1434
TaaI ACNGT 3 cut(s) 758, 997, 1498
TaiI ACGT 2 cut(s) 84, 434
TaqI TCGA 9 cut(s) 68, 248, 536, 543, 664, 686, 1397, 1557, 1584
TaqII GACCGA 1 cut(s) 1191
TasI AATT 9 cut(s) 478, 491, 559, 979, 1032, 1305, 1571, 1730, 1757
TatI WGTACW 2 cut(s) 318, 1648
TauI GCSGC 5 cut(s) 506, 550, 741, 775, 1213
TfiI GAWTC 7 cut(s) 101, 214, 598, 628, 801, 1216, 1414
Tru1I TTAA 6 cut(s) 380, 405, 477, 558, 969, 1433
Tru9I TTAA 6 cut(s) 380, 405, 477, 558, 969, 1433
TscAI CASTG 3 cut(s) 650, 1002, 1532
TseFI GTSAC 1 cut(s) 934
TseI GCWGC 4 cut(s) 157, 337, 908, 911
Tsp45I GTSAC 1 cut(s) 934
TspDTI ATGAA 6 cut(s) 807, 890, 1505, 1540, 1618, 1692
TspGWI ACGGA 3 cut(s) 499, 878, 1490
TspRI CASTG 3 cut(s) 650, 1002, 1532
Tth111I GACNNNGTC 2 cut(s) 758, 865
Vha464I CTTAAG 2 cut(s) 379, 404
VspI ATTAAT 1 cut(s) 477
XapI RAATTY 2 cut(s) 979, 1730
XbaI TCTAGA 1 cut(s) 1195
XcmI CCANNNNNNNNNTGG 1 cut(s) 1342
XhoI CTCGAG 1 cut(s) 1583
XmiI GTMKAC 3 cut(s) 392, 466, 1557
XspI CTAG 4 cut(s) 881, 1196, 1340, 1473
ZrmI AGTACT 1 cut(s) 320
Zsp2I ATGCAT 1 cut(s) 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.