Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000570

Physical Location & Seq

Genomic Coordinates Forward (+)

11440600 .. 11450786

Gene Structure 10187 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1146600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1773 bp

ATGGATAATGTCAATGAACTGTCATCATCCTTGAGCTTTGCCTCCTCCAATCTGTCAAATGGGTCTAGTAGTAACCATGCATCTGCCTCAGCCAGTTCCCCACCCGCGCCGAGTATTGAACATTACTTGAGTCTAAGCAAACTGAGCGATAATCTTGAGAGGCTGTTACTTGATCCTGAGTTTGACTACAGTGATGCTGAGATTGTTGTTGAGGGTAACACCGTGGGTGTCAATCGGTGTATATTGGCTTCCCGTAGTCAGTTTTTTCAGGAGCTTTTTAGGAAGGGGAGTGATGATTCGAACAAGGAAGGTAAACCGAGGTATCTCATGTCTGAATTGGTTCCAAACGGCGGGGTTGGCTATGAAGCCTTCAAGGTTTTCTTGAACTACTTGTACACTGGGAAGCTTAATCCATCCCCTCGAGAAGTGTCCACGTGCGTTGATGATGGTTGTCCGCATGATGCGTGCGGCCCTGCTATTAATTATGTTGTGGAATTGATGTATGCTTCTTCCACTTTCCAGATGAAGGAACTTGTTCAGCTTGTTCAGCGCCGTCTTTCAAACTTTGTGGAGAAAGCCCTTATTGAAGATGTAATACCAATTCTTAAAGCTGCCTTCCACTGCCAACTGAGCCAGCTGCTCTCACACTGCATCCAGAGGATAGCAAGGTCCGATCTTGACTACATGGCCATAGGGAAAGAGTTTCCGCATGAAGTTTCTAATGATATCAAATCGCTCCGTCTCAAATCTCACGAAAATGATGAATCAAGTATGATGGAAATTGAGTTGATAGAGGATAAGAGAATCAGGAGAATCCACAAGGCATTAGATTCTGATGACGTTGAACTAGTTGGACTTCTTCTCAAGGAATCTGATATTACGTTAGATGATGCTTATGCTCTCCACTATGCTGTTGCATACTGTGATCCTAAGGTTGTTAAGGAGGTTCTCAGTCTAGGCGAGGCTAACCTCAATCTTCGCAATTCCCGAGGGCATACTGTACTTCATGTGGCAGCCAGACGTAAGCTGCCAGCAGTGCTAGTTCCTCTTCTGGACAGGGGAGCCTCTGCCTTAGAAACTACTTCAGATGGTCAAACTCCTGTTGCAATTTGCCGGAGATTGACTAGACCAAAGGATTTTTTGGAGAACACAAAGCAGGGACAGGAATCTAACAAAGATCGATTATGCATCGAACTTCTAGAGAGAGAGATGCGGAGAAACTCAATCTCTGGGAACGTATCGATCATATCAGAGGTGATACCTGATGATTTGCACGTGAGGCTGGACTATCTAGAAAATAGAGTGGCATTTGCGCGGTTGTTGTTTCCTGCTGAGGCCAAGCTTGCTATGGAAATGGCTGATGCTCATTCCACATCAGTGTACACTGGCCTTGCGGCATCAAGAGGATCAAGTGGGAACTTACGAGAGGTTGATTTGAATGAAACACCCTCTGGACGAACCAAAAGACTACAAATAAGAATGCAAGCCCTTATTAAAACTGTGAAAATGGGGCGGCGTTTCTTCCCCAACTGCTCGGAAGTCCTTGATAAGTTTTTGGACGATGAGATGGACATGGCTGATTTCATCCTCGACAAGGGAACTCCGGAAGAGCAGAGAATCAAGAGGATGCGGTTCTTGGAACTTAAAGACGATGTACAGAAGGCATTTTGCAAGGACATGGCTGAAAATAACCGGTCGGTCTTATCAGCATCGTCGTCGTCGTCATCTTCTCCGAAGGACGGGGTAAATCACAAGGTAAGGAGAAGGTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008144 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009814 GO:0009816 GO:0009817 GO:0009966 GO:0009987 GO:0010498 GO:0010646 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0023051 GO:0030162 GO:0030163 GO:0031406 GO:0031461 GO:0031625 GO:0032991 GO:0033293 GO:0035821 GO:0036094 GO:0042562 GO:0042742 GO:0043161 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043207 GO:0043632 GO:0044003 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051246 GO:0051603 GO:0051701 GO:0051704 GO:0051707 GO:0051817 GO:0052031 GO:0052173 GO:0052200 GO:0052255 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052558 GO:0052559 GO:0052564 GO:0052572 GO:0060255 GO:0065007 GO:0071704 GO:0075136 GO:0080090 GO:0080185 GO:0097159 GO:0098542 GO:1901149 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234 GO:2000022

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Ank_2 BTB DUF3420 NPR1_like_C

Protein Analysis

591

Amino Acids

66.02

Weight (kDa)

5.87

Isoelectric Point (pI)

48.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB	PF00651	62 - 186	1.2e-11	BTB/POZ domain
DUF3420	PF11900	225 - 273	4.5e-09	Domain of unknown function (DUF3420)
Ank_2	PF12796	273 - 356	2.2e-07	Ankyrin repeats (3 copies)
Ank_5	PF13857	321 - 370	7.4e-06	Ankyrin repeats (many copies)
NPR1_like_C	PF12313	372 - 575	3.8e-84	NPR1/NIM1 like defence protein C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000570)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45110 AT5G45110
fragaria_vesca	FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_6g38820 FvH4_6g38820 FvH4_6g38821 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830
malus_domestica	MD05G1256300.v1.1 MD05G1256400.v1.1 MD09G1146600.v1.1 MD10G1236700.v1.1 MD10G1236800.v1.1 MD17G1133300.v1.1
prunus_persica	Prupe.3G178800_v2.0.a1 Prupe.3G178800_v2.0.a1 Prupe.3G178800_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1
pyrus_communis	pycom05g23540 pycom05g23550 pycom05g23560 pycom05g23570 pycom09g06690 pycom10g19800 pycom17g12780
rosa_chinensis	RchiOBHm_Chr2g0152421 RchiOBHm_Chr2g0152431 RchiOBHm_Chr2g0152441 RchiOBHm_Chr5g0019531
rosa_laevigata	RLG00000020632 RLG00000020633 RLG00000032453
rosa_multiflora	Rmu_co8414461.1_g000001 Rmu_sc0002759.1_g000050 Rmu_sc0005366.1_g000015 Rmu_sc0005366.1_g000016 Rmu_sc0005366.1_g000017 Rmu_sc0005366.1_g000020 Rmu_sc0005366.1_g000022
rosa_roxburghii	Rroxscaffold_1G00058530 Rroxscaffold_2G00095720 Rroxscaffold_2G00095730 Rroxscaffold_2G00095740
rosa_rugosa	Rorug02G0432800 Rorug05G0051600
rosa_samantha	Rh2AG494300 Rh2AG494400 Rh2AG494500 Rh2BG506600 Rh2BG506700 Rh2CG480600 Rh2CG480700 Rh2DG517700 Rh2DG517800 Rh2DG517900 Rh5AG142400 Rh5BG140800 Rh5CG152900 Rh5DG141500
rosa_wichuraiana	Rw2G040670 Rw2G040680 Rw2G040690 Rw5G012600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	107, 1315
AccIII	TCCGGA	1 cut(s)	1603
AclWI	GGATC	3 cut(s)	167, 920, 1414
AcoI	YGGCCR	1 cut(s)	687
AcuI	CTGAAG	1 cut(s)	1068
AcvI	CACGTG	2 cut(s)	435, 1276
AfaI	GTAC	4 cut(s)	395, 1002, 1382, 1656
AfiI	CCNNNNNNNGG	4 cut(s)	350, 526, 1594, 1739
AgeI	ACCGGT	1 cut(s)	1692
AgsI	TTSAA	7 cut(s)	119, 373, 385, 561, 587, 845, 1438
AhlI	ACTAGT	1 cut(s)	847
AleI	CACNNNNGTG	1 cut(s)	1376
AluBI	AGCT	8 cut(s)	36, 274, 406, 541, 611, 637, 1027, 1342
AluI	AGCT	8 cut(s)	36, 274, 406, 541, 611, 637, 1027, 1342
Alw26I	GTCTC	1 cut(s)	746
AlwI	GGATC	3 cut(s)	167, 920, 1414
Ama87I	CYCGRG	2 cut(s)	420, 987
Aor13HI	TCCGGA	1 cut(s)	1603
AoxI	GGCC	4 cut(s)	469, 687, 1335, 1387
ApeKI	GCWGC	4 cut(s)	611, 637, 1013, 1027
AseI	ATTAAT	1 cut(s)	480
AsiGI	ACCGGT	1 cut(s)	1692
Asp700I	GAANNNNTTC	2 cut(s)	339, 534
AspLEI	GCGC	3 cut(s)	109, 552, 1315
AspS9I	GGNCC	2 cut(s)	470, 669
AsuHPI	GGTGA	1 cut(s)	1267
AsuII	TTCGAA	1 cut(s)	299
AvaI	CYCGRG	2 cut(s)	420, 987
AvaII	GGWCC	1 cut(s)	669
AxyI	CCTNAGG	1 cut(s)	930
BaeI	ACNNNNGTAYC	2 cut(s)	305, 338
BalI	TGGCCA	1 cut(s)	689
BarI	GAAGNNNNNNTAC	4 cut(s)	232, 264, 579, 611
BbrPI	CACGTG	2 cut(s)	435, 1276
BbvCI	CCTCAGC	2 cut(s)	88, 1332
BbvI	GCAGC	4 cut(s)	598, 624, 1014, 1025
BccI	CCATC	5 cut(s)	421, 440, 769, 1082, 1561
BceAI	ACGGC	2 cut(s)	364, 537
BcgI	CGANNNNNNTGC	2 cut(s)	1698, 1732
BcoDI	GTCTC	1 cut(s)	746
BcuI	ACTAGT	1 cut(s)	847
BfaI	CTAG	7 cut(s)	66, 848, 956, 1040, 1125, 1199, 1292
BfmI	CTRYAG	1 cut(s)	187
BfoI	RGCGCY	1 cut(s)	553
BisI	GCNGC	7 cut(s)	469, 612, 638, 1014, 1028, 1395, 1514
BlsI	GCNGC	7 cut(s)	470, 613, 639, 1015, 1029, 1396, 1515
Bme18I	GGWCC	1 cut(s)	669
BmeT110I	CYCGRG	2 cut(s)	420, 987
BmgT120I	GGNCC	2 cut(s)	470, 669
BmiI	GGNNCC	2 cut(s)	342, 1063
BmrI	ACTGGG	1 cut(s)	408
BmuI	ACTGGG	1 cut(s)	408
Bpu10I	CCTNAGC	2 cut(s)	88, 1332
Bpu14I	TTCGAA	1 cut(s)	299
BpuEI	CTTGAG	4 cut(s)	52, 148, 176, 848
Bsa29I	ATCGAT	2 cut(s)	1180, 1241
BsaAI	YACGTR	2 cut(s)	435, 1276
BsaJI	CCNNGG	3 cut(s)	222, 317, 988
BsaWI	WCCGGW	2 cut(s)	1603, 1692
BsaXI	ACNNNNNCTCC	1 cut(s)	1753
Bsc4I	CCNNNNNNNGG	4 cut(s)	350, 526, 1594, 1739
Bse118I	RCCGGY	1 cut(s)	1692
Bse1I	ACTGG	3 cut(s)	93, 403, 1390
Bse21I	CCTNAGG	1 cut(s)	930
BseAI	TCCGGA	1 cut(s)	1603
BseCI	ATCGAT	2 cut(s)	1180, 1241
BseDI	CCNNGG	3 cut(s)	222, 317, 988
BseGI	GGATG	5 cut(s)	26, 413, 651, 1584, 1632
BseLI	CCNNNNNNNGG	4 cut(s)	350, 526, 1594, 1739
BseMII	CTCAG	7 cut(s)	102, 134, 168, 189, 620, 964, 1323
BseNI	ACTGG	3 cut(s)	93, 403, 1390
BseRI	GAGGAG	1 cut(s)	34
BseXI	GCAGC	4 cut(s)	598, 624, 1014, 1025
Bsh1236I	CGCG	2 cut(s)	107, 1315
Bsh1285I	CGRYCG	1 cut(s)	1697
BshFI	GGCC	4 cut(s)	471, 689, 1337, 1389
BshTI	ACCGGT	1 cut(s)	1692
BshVI	ATCGAT	2 cut(s)	1180, 1241
BsiEI	CGRYCG	1 cut(s)	1697
BsiHKCI	CYCGRG	2 cut(s)	420, 987
BsiSI	CCGG	3 cut(s)	1114, 1604, 1693
BslFI	GGGAC	1 cut(s)	1173
BslI	CCNNNNNNNGG	4 cut(s)	350, 526, 1594, 1739
BsmAI	GTCTC	1 cut(s)	746
BsmBI	CGTCTC	1 cut(s)	746
BsmFI	GGGAC	1 cut(s)	1173
BsmI	GAATGC	1 cut(s)	1485
BsnI	GGCC	4 cut(s)	471, 689, 1337, 1389
BsoBI	CYCGRG	2 cut(s)	420, 987
Bsp119I	TTCGAA	1 cut(s)	299
Bsp13I	TCCGGA	1 cut(s)	1603
Bsp1407I	TGTACA	3 cut(s)	393, 1380, 1654
Bsp143I	GATC	6 cut(s)	172, 673, 925, 1177, 1242, 1406
BspANI	GGCC	4 cut(s)	471, 689, 1337, 1389
BspCNI	CTCAG	7 cut(s)	101, 135, 169, 190, 621, 963, 1324
BspDI	ATCGAT	2 cut(s)	1180, 1241
BspEI	TCCGGA	1 cut(s)	1603
BspFNI	CGCG	2 cut(s)	107, 1315
BspLI	GGNNCC	2 cut(s)	342, 1063
BspPI	GGATC	3 cut(s)	167, 920, 1414
BspQI	GCTCTTC	1 cut(s)	1602
BspT104I	TTCGAA	1 cut(s)	299
BsrFI	RCCGGY	1 cut(s)	1692
BsrGI	TGTACA	3 cut(s)	393, 1380, 1654
BsrI	ACTGG	3 cut(s)	93, 403, 1390
BssAI	RCCGGY	1 cut(s)	1692
BssECI	CCNNGG	3 cut(s)	222, 317, 988
BssMI	GATC	6 cut(s)	172, 673, 925, 1177, 1242, 1406
Bst4CI	ACNGT	6 cut(s)	21, 191, 223, 923, 1000, 1501
Bst6I	CTCTTC	2 cut(s)	1053, 1602
BstAUI	TGTACA	3 cut(s)	393, 1380, 1654
BstBAI	YACGTR	2 cut(s)	435, 1276
BstBI	TTCGAA	1 cut(s)	299
BstC8I	GCNNGC	5 cut(s)	466, 635, 1032, 1344, 1485
BstDSI	CCRYGG	1 cut(s)	222
BstENI	CCTNNNNNAGG	1 cut(s)	1592
BstF5I	GGATG	5 cut(s)	26, 413, 651, 1584, 1632
BstFNI	CGCG	2 cut(s)	107, 1315
BstH2I	RGCGCY	1 cut(s)	553
BstHHI	GCGC	3 cut(s)	109, 552, 1315
BstKTI	GATC	6 cut(s)	175, 676, 928, 1180, 1245, 1409
BstMAI	GTCTC	1 cut(s)	746
BstMBI	GATC	6 cut(s)	172, 673, 925, 1177, 1242, 1406
BstMCI	CGRYCG	1 cut(s)	1697
BstMWI	GCNNNNNNNGC	7 cut(s)	144, 357, 547, 630, 1036, 1279, 1343
BstSFI	CTRYAG	1 cut(s)	187
BstUI	CGCG	2 cut(s)	107, 1315
BstV1I	GCAGC	4 cut(s)	598, 624, 1014, 1025
Bsu15I	ATCGAT	2 cut(s)	1180, 1241
Bsu36I	CCTNAGG	1 cut(s)	930
BsuRI	GGCC	4 cut(s)	471, 689, 1337, 1389
BsuTUI	ATCGAT	2 cut(s)	1180, 1241
BtgI	CCRYGG	1 cut(s)	222
BtsCI	GGATG	5 cut(s)	26, 413, 651, 1584, 1632
BtsI	GCAGTG	3 cut(s)	619, 646, 1041
BtsIMutI	CAGTG	7 cut(s)	196, 396, 619, 646, 1041, 1383, 1383
Cac8I	GCNNGC	5 cut(s)	466, 635, 1032, 1344, 1485
CfoI	GCGC	3 cut(s)	109, 552, 1315
Cfr10I	RCCGGY	1 cut(s)	1692
Cfr13I	GGNCC	2 cut(s)	470, 669
ClaI	ATCGAT	2 cut(s)	1180, 1241
Csp6I	GTAC	4 cut(s)	394, 1001, 1381, 1655
CspAI	ACCGGT	1 cut(s)	1692
CspCI	CAANNNNNGTGG	4 cut(s)	421, 456, 549, 584
CviAII	CATG	8 cut(s)	77, 328, 458, 685, 710, 1007, 1573, 1678
CviQI	GTAC	4 cut(s)	394, 1001, 1381, 1655
DpnI	GATC	6 cut(s)	174, 675, 927, 1179, 1244, 1408
DpnII	GATC	6 cut(s)	172, 673, 925, 1177, 1242, 1406
EaeI	YGGCCR	1 cut(s)	687
Eam1104I	CTCTTC	2 cut(s)	1053, 1602
EarI	CTCTTC	2 cut(s)	1053, 1602
Eco32I	GATATC	1 cut(s)	727
Eco47I	GGWCC	1 cut(s)	669
Eco57I	CTGAAG	1 cut(s)	1068
Eco72I	CACGTG	2 cut(s)	435, 1276
Eco81I	CCTNAGG	1 cut(s)	930
Eco88I	CYCGRG	2 cut(s)	420, 987
EcoNI	CCTNNNNNAGG	1 cut(s)	1592
EcoRV	GATATC	1 cut(s)	727
EcoT22I	ATGCAT	2 cut(s)	82, 1190
Esp3I	CGTCTC	1 cut(s)	746
FaeI	CATG	8 cut(s)	80, 331, 461, 688, 713, 1010, 1576, 1681
FalI	AAGNNNNNCTT	2 cut(s)	365, 397
FaqI	GGGAC	1 cut(s)	1173
FatI	CATG	8 cut(s)	76, 327, 457, 684, 709, 1006, 1572, 1677
FauI	CCCGC	2 cut(s)	112, 344
Fnu4HI	GCNGC	7 cut(s)	469, 612, 638, 1014, 1028, 1395, 1514
FokI	GGATG	5 cut(s)	13, 400, 638, 1571, 1639
Fsp4HI	GCNGC	7 cut(s)	469, 612, 638, 1014, 1028, 1395, 1514
FspBI	CTAG	7 cut(s)	66, 848, 956, 1040, 1125, 1199, 1292
GlaI	GCGC	3 cut(s)	108, 551, 1314
GluI	GCNGC	7 cut(s)	469, 612, 638, 1014, 1028, 1395, 1514
HaeII	RGCGCY	1 cut(s)	553
HaeIII	GGCC	4 cut(s)	471, 689, 1337, 1389
HapII	CCGG	3 cut(s)	1114, 1604, 1693
HhaI	GCGC	3 cut(s)	109, 552, 1315
Hin1II	CATG	8 cut(s)	80, 331, 461, 688, 713, 1010, 1576, 1681
Hin6I	GCGC	3 cut(s)	107, 550, 1313
HinP1I	GCGC	3 cut(s)	107, 550, 1313
HindIII	AAGCTT	2 cut(s)	404, 1340
HinfI	GANTC	9 cut(s)	130, 296, 764, 804, 813, 830, 869, 1166, 1617
HpaII	CCGG	3 cut(s)	1114, 1604, 1693
HphI	GGTGA	1 cut(s)	1267
Hpy166II	GTNNAC	5 cut(s)	314, 396, 432, 1381, 1383
Hpy188I	TCNGA	8 cut(s)	334, 673, 835, 874, 1087, 1252, 1537, 1734
Hpy8I	GTNNAC	5 cut(s)	314, 396, 432, 1381, 1383
Hpy99I	CGWCG	3 cut(s)	1717, 1720, 1723
HpyAV	CCTTC	8 cut(s)	277, 302, 379, 520, 625, 1654, 1729, 1758
HpyCH4III	ACNGT	6 cut(s)	21, 191, 223, 923, 1000, 1501
HpyCH4IV	ACGT	6 cut(s)	434, 840, 881, 1021, 1236, 1275
HpyCH4V	TGCA	8 cut(s)	80, 651, 917, 1106, 1188, 1273, 1483, 1671
HpyF10VI	GCNNNNNNNGC	7 cut(s)	144, 357, 547, 630, 1036, 1279, 1343
HpySE526I	ACGT	6 cut(s)	434, 840, 881, 1021, 1236, 1275
Hsp92II	CATG	8 cut(s)	80, 331, 461, 688, 713, 1010, 1576, 1681
HspAI	GCGC	3 cut(s)	107, 550, 1313
Kpn2I	TCCGGA	1 cut(s)	1603
Kzo9I	GATC	6 cut(s)	172, 673, 925, 1177, 1242, 1406
LguI	GCTCTTC	1 cut(s)	1602
LmnI	GCTCC	3 cut(s)	271, 741, 1061
Lsp1109I	GCAGC	4 cut(s)	598, 624, 1014, 1025
MaeI	CTAG	7 cut(s)	66, 848, 956, 1040, 1125, 1199, 1292
MaeII	ACGT	6 cut(s)	434, 840, 881, 1021, 1236, 1275
MaeIII	GTNAC	3 cut(s)	71, 165, 215
MalI	GATC	6 cut(s)	174, 675, 927, 1179, 1244, 1408
MboI	GATC	6 cut(s)	172, 673, 925, 1177, 1242, 1406
MboII	GAAGA	8 cut(s)	501, 599, 851, 968, 1040, 1513, 1619, 1719
MlsI	TGGCCA	1 cut(s)	689
MluCI	AATT	7 cut(s)	335, 481, 494, 600, 780, 982, 1107
MluNI	TGGCCA	1 cut(s)	689
MlyI	GAGTC	1 cut(s)	139
MmeI	TCCRAC	1 cut(s)	832
Mox20I	TGGCCA	1 cut(s)	689
Mph1103I	ATGCAT	2 cut(s)	82, 1190
MroI	TCCGGA	1 cut(s)	1603
MroXI	GAANNNNTTC	2 cut(s)	339, 534
MscI	TGGCCA	1 cut(s)	689
MseI	TTAA	6 cut(s)	408, 480, 606, 939, 1494, 1644
MslI	CAYNNNNRTG	2 cut(s)	756, 1376
Msp20I	TGGCCA	1 cut(s)	689
MspA1I	CMGCKG	1 cut(s)	637
MspI	CCGG	3 cut(s)	1114, 1604, 1693
Mva1269I	GAATGC	1 cut(s)	1485
MvnI	CGCG	2 cut(s)	107, 1315
MwoI	GCNNNNNNNGC	7 cut(s)	144, 357, 547, 630, 1036, 1279, 1343
NdeII	GATC	6 cut(s)	172, 673, 925, 1177, 1242, 1406
NlaIII	CATG	8 cut(s)	80, 331, 461, 688, 713, 1010, 1576, 1681
NlaIV	GGNNCC	2 cut(s)	342, 1063
NmeAIII	GCCGAG	1 cut(s)	135
NsiI	ATGCAT	2 cut(s)	82, 1190
NspV	TTCGAA	1 cut(s)	299
OliI	CACNNNNGTG	1 cut(s)	1376
PaeR7I	CTCGAG	1 cut(s)	420
PciSI	GCTCTTC	1 cut(s)	1602
PcsI	WCGNNNNNNNCGW	2 cut(s)	985, 1718
PctI	GAATGC	1 cut(s)	1485
PdmI	GAANNNNTTC	2 cut(s)	339, 534
PfeI	GAWTC	8 cut(s)	296, 764, 804, 813, 830, 869, 1166, 1617
PinAI	ACCGGT	1 cut(s)	1692
PkrI	GCNGC	7 cut(s)	470, 613, 639, 1015, 1029, 1396, 1515
PleI	GAGTC	1 cut(s)	138
PmaCI	CACGTG	2 cut(s)	435, 1276
PmlI	CACGTG	2 cut(s)	435, 1276
PpsI	GAGTC	1 cut(s)	138
Ppu21I	YACGTR	2 cut(s)	435, 1276
PshBI	ATTAAT	1 cut(s)	480
PspCI	CACGTG	2 cut(s)	435, 1276
PspN4I	GGNNCC	2 cut(s)	342, 1063
PspPI	GGNCC	2 cut(s)	470, 669
PsrI	GAACNNNNNNTAC	2 cut(s)	377, 409
PvuII	CAGCTG	1 cut(s)	637
RsaI	GTAC	4 cut(s)	395, 1002, 1382, 1656
RsaNI	GTAC	4 cut(s)	394, 1001, 1381, 1655
RseI	CAYNNNNRTG	2 cut(s)	756, 1376
SapI	GCTCTTC	1 cut(s)	1602
SaqAI	TTAA	6 cut(s)	408, 480, 606, 939, 1494, 1644
SatI	GCNGC	7 cut(s)	469, 612, 638, 1014, 1028, 1395, 1514
Sau3AI	GATC	6 cut(s)	172, 673, 925, 1177, 1242, 1406
Sau96I	GGNCC	2 cut(s)	470, 669
SchI	GAGTC	1 cut(s)	139
SfcI	CTRYAG	1 cut(s)	187
Sfr274I	CTCGAG	1 cut(s)	420
SfuI	TTCGAA	1 cut(s)	299
SinI	GGWCC	1 cut(s)	669
SlaI	CTCGAG	1 cut(s)	420
SmiMI	CAYNNNNRTG	2 cut(s)	756, 1376
SmlI	CTYRAG	5 cut(s)	31, 127, 155, 420, 863
SmoI	CTYRAG	5 cut(s)	31, 127, 155, 420, 863
SpeI	ACTAGT	1 cut(s)	847
Sse9I	AATT	7 cut(s)	335, 481, 494, 600, 780, 982, 1107
SspMI	CTAG	7 cut(s)	66, 848, 956, 1040, 1125, 1199, 1292
TaaI	ACNGT	6 cut(s)	21, 191, 223, 923, 1000, 1501
TaiI	ACGT	6 cut(s)	437, 843, 884, 1024, 1239, 1278
TaqI	TCGA	6 cut(s)	299, 421, 1180, 1191, 1241, 1590
TaqII	GACCGA	1 cut(s)	1687
TasI	AATT	7 cut(s)	335, 481, 494, 600, 780, 982, 1107
TatI	WGTACW	4 cut(s)	393, 1000, 1380, 1654
TauI	GCSGC	3 cut(s)	471, 1397, 1516
TfiI	GAWTC	8 cut(s)	296, 764, 804, 813, 830, 869, 1166, 1617
Tru1I	TTAA	6 cut(s)	408, 480, 606, 939, 1494, 1644
Tru9I	TTAA	6 cut(s)	408, 480, 606, 939, 1494, 1644
TscAI	CASTG	7 cut(s)	196, 403, 626, 653, 1041, 1383, 1390
TseI	GCWGC	4 cut(s)	611, 637, 1013, 1027
TspDTI	ATGAA	8 cut(s)	30, 378, 539, 726, 777, 995, 1455, 1573
TspGWI	ACGGA	1 cut(s)	728
TspRI	CASTG	7 cut(s)	196, 403, 626, 653, 1041, 1383, 1390
VpaK11BI	GGWCC	1 cut(s)	669
VspI	ATTAAT	1 cut(s)	480
XagI	CCTNNNNNAGG	1 cut(s)	1592
XbaI	TCTAGA	2 cut(s)	1198, 1291
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1345
XhoI	CTCGAG	1 cut(s)	420
XmnI	GAANNNNTTC	2 cut(s)	339, 534
XspI	CTAG	7 cut(s)	66, 848, 956, 1040, 1125, 1199, 1292
Zsp2I	ATGCAT	2 cut(s)	82, 1190

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.