Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0000570

Physical Location & Seq

Genomic Coordinates Reverse (-)

74151586 .. 74156396

Gene Structure 4811 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG517900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1782 bp

ATGGATCATTTGAATGACCTTTCGTCATCTTTGAGCTTTGCGTCTTCTTATCTATCGAATGGATCAAGTGGTAACTATGTATCTGCCTCGGCCAATTCTCCAAGTACAGAGCATTTGAGTCTGAGCAGACTCAGTGATAACCTGGAGAGGCTGTTGCTTGATGCTCAATATGACTATGGTGATGCGGACATTGTTGTTGAAGGGGTCGCGGTTGGTGTCAATCGGTGTATATTGGCTGCACGCAGTCAGTTTTTCCACGAGCTTTTTAAGAAGGGGAATGATGATTCGAAGAAGGAAGGTAAACCGCAGTATATGATGTCTGAATTGGTTCCTTTCGGTGGAGTTGGCTATGAAGCATTCAAGGTAGTCTTGAACTACTTGTACACTGGAAAACTTAAGCCACCGCCTCCAGAAGTATCAACTGTATCAACATGTGTTGATGATAATTGTGCCCATGATGCATGCGGCCCTGCTATTAATTACGCTGTGGAATTAATGTATGCTGCTGCCACTTTCCAGATGAAAGAACTTGTTCATGTTGTGCAGCGCCGGCTTACTAACTTTGTCGAGAAAGCTCATGTTGAAGACGTGATCCCGATTCTTGTAGCCTCTTTCCACTGCAAACAGAGTCAGCTGTTGTCACACTGCATCCAGAGGGTAGCAAAGTCAGATCTTGACAATGTGGTTCTAGAAAAAGAGCTTCCTCATGAAGTTTTTGATATTATCAAATCACTCCGTCTCGAATCTCAGCAAATGGATGAATCAAGTATGCTGGAAATGGAGCCAGGGAAGGAAAAGAGGCTTAAAAGCATCGGGAGAATCCACAAGGCATTAGACTCTGATGATGTTGAACTGGTTACTCTTCTTCTAAAAGAGTCTGATTCTATTTCATTAGATGATGCTAATGCTCTCCACTATGCTGTTGCATACTGTGATCCAAAAATTGTTAAGGAGGTTCTTGGTCTACGTCTGGCGAATACCAATCTTCGAAATGCCCGAGGACATACTGTACTTCATGTGGCTGCAAGGCGTAAGGAACCCGCAGTCCTAGTTCCTCTTCTGAACAGCGGAGCCTCTGCCTTAGAAACTACATTAGATGGTCAAAGTGCTGTTGCAATTTGCCGGAGGTTGACTAGACCAAAGGATTATAATGAGAACACAAAGCAGGGAGAGGTGTCTAACAAAGACCGAATATGCATAGATCTTCTAGAGAGAGAGATGCAAAGAAATTCGATGGCTGTGGACATGTCAAACACATCACAGGTGATAGCTGATGATTTGAATGTGAGGCTGGACTATTTTGAAAATAGAGTGGCATTTGCACGGTTGTTGTTTCCTGCTGAAGCCAAGCTTGCTATGGAAATGGCGGATCATCCAACATCAGGGTACACTGGCCTTTTAGCATCAAAAGGCTCTAGTGGGAACTTACGAGAGGTCGATTTGAATGAAACACCCTCTGTACGATCCAAAAGACTCCAAGAAAAATTGCATGCGCTAATTAAAACAGTGCAAATGGGACGGCGCTTCTTCCCCCATTACTCAGAAGTTCTTGATAAGTTTCTGGATGATGAGATGGATATGGCTGATTACTTCCTTGAGAAAGGAACTCCTGAAGAGCAGAAAAGCAAGAAGATGCGCTTCTTGGAGCTTAAAGATGATGTACAGAAAGCATTTTGCAGGGATGTGGCTGAAAAACGGGGGCCAGATTTGACGGCCTCATCATCATCCTCATCTTCTCCGAAGGAGGGTGTTAATCGTAAGGTTAGGAAAAGGTCAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008144 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009814 GO:0009816 GO:0009817 GO:0009966 GO:0009987 GO:0010498 GO:0010646 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0023051 GO:0030162 GO:0030163 GO:0031406 GO:0031461 GO:0031625 GO:0032991 GO:0033293 GO:0035821 GO:0036094 GO:0042562 GO:0042742 GO:0043161 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043207 GO:0043632 GO:0044003 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051246 GO:0051603 GO:0051701 GO:0051704 GO:0051707 GO:0051817 GO:0052031 GO:0052173 GO:0052200 GO:0052255 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052558 GO:0052559 GO:0052564 GO:0052572 GO:0060255 GO:0065007 GO:0071704 GO:0075136 GO:0080090 GO:0080185 GO:0097159 GO:0098542 GO:1901149 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234 GO:2000022

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Ank_2 BTB DUF3420 NPR1_like_C

Protein Analysis

593

Amino Acids

66.12

Weight (kDa)

5.95

Isoelectric Point (pI)

40.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB	PF00651	56 - 144	6.3e-11	BTB/POZ domain
DUF3420	PF11900	224 - 275	5.1e-10	Domain of unknown function (DUF3420)
Ank_2	PF12796	275 - 359	4.4e-07	Ankyrin repeats (3 copies)
NPR1_like_C	PF12313	375 - 577	1.9e-75	NPR1/NIM1 like defence protein C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000570)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45110 AT5G45110
fragaria_vesca	FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_6g38820 FvH4_6g38820 FvH4_6g38821 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830
malus_domestica	MD05G1256300.v1.1 MD05G1256400.v1.1 MD09G1146600.v1.1 MD10G1236700.v1.1 MD10G1236800.v1.1 MD17G1133300.v1.1
prunus_persica	Prupe.3G178800_v2.0.a1 Prupe.3G178800_v2.0.a1 Prupe.3G178800_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1
pyrus_communis	pycom05g23540 pycom05g23550 pycom05g23560 pycom05g23570 pycom09g06690 pycom10g19800 pycom17g12780
rosa_chinensis	RchiOBHm_Chr2g0152421 RchiOBHm_Chr2g0152431 RchiOBHm_Chr2g0152441 RchiOBHm_Chr5g0019531
rosa_laevigata	RLG00000020632 RLG00000020633 RLG00000032453
rosa_multiflora	Rmu_co8414461.1_g000001 Rmu_sc0002759.1_g000050 Rmu_sc0005366.1_g000015 Rmu_sc0005366.1_g000016 Rmu_sc0005366.1_g000017 Rmu_sc0005366.1_g000020 Rmu_sc0005366.1_g000022
rosa_roxburghii	Rroxscaffold_1G00058530 Rroxscaffold_2G00095720 Rroxscaffold_2G00095730 Rroxscaffold_2G00095740
rosa_rugosa	Rorug02G0432800 Rorug05G0051600
rosa_samantha	Rh2AG494300 Rh2AG494400 Rh2AG494500 Rh2BG506600 Rh2BG506700 Rh2CG480600 Rh2CG480700 Rh2DG517700 Rh2DG517800 Rh2DG517900 Rh5AG142400 Rh5BG140800 Rh5CG152900 Rh5DG141500
rosa_wichuraiana	Rw2G040670 Rw2G040680 Rw2G040690 Rw5G012600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1149
AccI	GTMKAC	1 cut(s)	964
AccII	CGCG	1 cut(s)	209
AciI	CCGC	8 cut(s)	185, 209, 305, 404, 465, 1041, 1068, 1367
AclWI	GGATC	6 cut(s)	12, 70, 586, 929, 1377, 1458
AcoI	YGGCCR	1 cut(s)	90
AcsI	RAATTY	1 cut(s)	1228
AcuI	CTGAAG	2 cut(s)	1362, 1632
AfaI	GTAC	6 cut(s)	106, 383, 1011, 1388, 1461, 1662
AfiI	CCNNNNNNNGG	3 cut(s)	338, 1382, 1745
AflII	CTTAAG	1 cut(s)	395
AflIII	ACRYGT	2 cut(s)	431, 1245
AgsI	TTSAA	9 cut(s)	13, 200, 361, 373, 584, 851, 1282, 1304, 1444
AhdI	GACNNNNNGTC	1 cut(s)	22
AjiI	CACGTC	1 cut(s)	589
AjnI	CCWGG	2 cut(s)	141, 784
AloI	GAACNNNNNNTCC	2 cut(s)	1029, 1061
AluBI	AGCT	8 cut(s)	36, 262, 575, 634, 700, 1271, 1351, 1648
AluI	AGCT	8 cut(s)	36, 262, 575, 634, 700, 1271, 1351, 1648
Alw26I	GTCTC	1 cut(s)	743
AlwI	GGATC	6 cut(s)	12, 70, 586, 929, 1377, 1458
Ama87I	CYCGRG	1 cut(s)	996
AoxI	GGCC	5 cut(s)	90, 466, 1393, 1700, 1713
ApeKI	GCWGC	5 cut(s)	236, 503, 506, 544, 1022
ApoI	RAATTY	1 cut(s)	1228
AseI	ATTAAT	2 cut(s)	477, 494
Asp700I	GAANNNNTTC	2 cut(s)	327, 531
AspLEI	GCGC	4 cut(s)	549, 1495, 1524, 1638
AspS9I	GGNCC	2 cut(s)	467, 1700
AsuHPI	GGTGA	2 cut(s)	191, 1276
AsuII	TTCGAA	2 cut(s)	287, 988
AvaI	CYCGRG	1 cut(s)	996
BaeGI	GKGCMC	1 cut(s)	454
BauI	CACGAG	1 cut(s)	257
BbsI	GAAGAC	2 cut(s)	36, 591
BbvI	GCAGC	5 cut(s)	223, 490, 493, 556, 1009
BccI	CCATC	3 cut(s)	1091, 1228, 1567
BceAI	ACGGC	2 cut(s)	1535, 1728
BciT130I	CCWGG	2 cut(s)	143, 786
BcoDI	GTCTC	1 cut(s)	743
BfaI	CTAG	5 cut(s)	689, 1049, 1134, 1208, 1416
BfoI	RGCGCY	2 cut(s)	550, 1525
BfrI	CTTAAG	1 cut(s)	395
BglII	AGATCT	2 cut(s)	670, 1201
BisI	GCNGC	6 cut(s)	237, 466, 504, 507, 545, 1023
BlsI	GCNGC	6 cut(s)	238, 467, 505, 508, 546, 1024
Bme1390I	CCNGG	2 cut(s)	143, 786
BmeRI	GACNNNNNGTC	1 cut(s)	22
BmeT110I	CYCGRG	1 cut(s)	996
BmgBI	CACGTC	1 cut(s)	589
BmgT120I	GGNCC	2 cut(s)	467, 1700
BmiI	GGNNCC	5 cut(s)	330, 783, 1038, 1072, 1701
BmrFI	CCNGG	2 cut(s)	143, 786
BmsI	GCATC	9 cut(s)	151, 172, 448, 657, 819, 889, 1209, 1412, 1623
BpiI	GAAGAC	2 cut(s)	36, 591
BpmI	CTGGAG	2 cut(s)	164, 393
Bpu14I	TTCGAA	2 cut(s)	287, 988
BpuEI	CTTGAG	1 cut(s)	1616
BsaJI	CCNNGG	3 cut(s)	87, 785, 997
BsaXI	ACNNNNNCTCC	2 cut(s)	137, 167
Bsc4I	CCNNNNNNNGG	3 cut(s)	338, 1382, 1745
Bse118I	RCCGGY	1 cut(s)	549
Bse1I	ACTGG	3 cut(s)	391, 858, 1396
BseBI	CCWGG	2 cut(s)	143, 786
BseDI	CCNNGG	3 cut(s)	87, 785, 997
BseGI	GGATG	6 cut(s)	648, 763, 1372, 1570, 1687, 1724
BseLI	CCNNNNNNNGG	3 cut(s)	338, 1382, 1745
BseMII	CTCAG	4 cut(s)	113, 145, 761, 1554
BseNI	ACTGG	3 cut(s)	391, 858, 1396
BseSI	GKGCMC	1 cut(s)	454
BseXI	GCAGC	5 cut(s)	223, 490, 493, 556, 1009
BsgI	GTGCAG	2 cut(s)	222, 563
Bsh1236I	CGCG	1 cut(s)	209
BshFI	GGCC	5 cut(s)	92, 468, 1395, 1702, 1715
BsiHKCI	CYCGRG	1 cut(s)	996
BsiSI	CCGG	2 cut(s)	550, 1123
BslFI	GGGAC	1 cut(s)	1530
BslI	CCNNNNNNNGG	3 cut(s)	338, 1382, 1745
BsmAI	GTCTC	1 cut(s)	743
BsmBI	CGTCTC	1 cut(s)	743
BsmFI	GGGAC	1 cut(s)	1530
BsmI	GAATGC	1 cut(s)	356
BsnI	GGCC	5 cut(s)	92, 468, 1395, 1702, 1715
BsoBI	CYCGRG	1 cut(s)	996
Bsp119I	TTCGAA	2 cut(s)	287, 988
Bsp1286I	GDGCHC	1 cut(s)	454
Bsp1407I	TGTACA	2 cut(s)	381, 1660
Bsp143I	GATC	8 cut(s)	4, 62, 591, 670, 934, 1201, 1369, 1463
BspACI	CCGC	8 cut(s)	185, 209, 305, 404, 465, 1041, 1068, 1367
BspANI	GGCC	5 cut(s)	92, 468, 1395, 1702, 1715
BspCNI	CTCAG	4 cut(s)	114, 144, 760, 1553
BspFNI	CGCG	1 cut(s)	209
BspHI	TCATGA	1 cut(s)	706
BspLI	GGNNCC	5 cut(s)	330, 783, 1038, 1072, 1701
BspPI	GGATC	6 cut(s)	12, 70, 586, 929, 1377, 1458
BspQI	GCTCTTC	1 cut(s)	1608
BspT104I	TTCGAA	2 cut(s)	287, 988
BspTI	CTTAAG	1 cut(s)	395
BsrFI	RCCGGY	1 cut(s)	549
BsrGI	TGTACA	2 cut(s)	381, 1660
BsrI	ACTGG	3 cut(s)	391, 858, 1396
BssAI	RCCGGY	1 cut(s)	549
BssECI	CCNNGG	3 cut(s)	87, 785, 997
BssMI	GATC	8 cut(s)	4, 62, 591, 670, 934, 1201, 1369, 1463
BssSI	CACGAG	1 cut(s)	257
Bst2BI	CACGAG	1 cut(s)	257
Bst2UI	CCWGG	2 cut(s)	143, 786
Bst4CI	ACNGT	5 cut(s)	424, 932, 1009, 1326, 1507
Bst6I	CTCTTC	3 cut(s)	867, 1062, 1608
BstAFI	CTTAAG	1 cut(s)	395
BstAUI	TGTACA	2 cut(s)	381, 1660
BstBI	TTCGAA	2 cut(s)	287, 988
BstC8I	GCNNGC	5 cut(s)	241, 463, 551, 1353, 1491
BstDEI	CTNAG	5 cut(s)	122, 131, 747, 1081, 1540
BstF5I	GGATG	6 cut(s)	648, 763, 1372, 1570, 1687, 1724
BstFNI	CGCG	1 cut(s)	209
BstH2I	RGCGCY	2 cut(s)	550, 1525
BstHHI	GCGC	4 cut(s)	549, 1495, 1524, 1638
BstKTI	GATC	8 cut(s)	7, 65, 594, 673, 937, 1204, 1372, 1466
BstMAI	GTCTC	1 cut(s)	743
BstMBI	GATC	8 cut(s)	4, 62, 591, 670, 934, 1201, 1369, 1463
BstMWI	GCNNNNNNNGC	3 cut(s)	458, 550, 1352
BstNI	CCWGG	2 cut(s)	143, 786
BstNSI	RCATGY	4 cut(s)	435, 465, 1249, 1493
BstSCI	CCNGG	2 cut(s)	141, 784
BstSLI	GKGCMC	1 cut(s)	454
BstUI	CGCG	1 cut(s)	209
BstV1I	GCAGC	5 cut(s)	223, 490, 493, 556, 1009
BstV2I	GAAGAC	2 cut(s)	36, 591
BstX2I	RGATCY	2 cut(s)	670, 1201
BstYI	RGATCY	2 cut(s)	670, 1201
BsuRI	GGCC	5 cut(s)	92, 468, 1395, 1702, 1715
BtrI	CACGTC	1 cut(s)	589
BtsCI	GGATG	6 cut(s)	648, 763, 1372, 1570, 1687, 1724
BtsI	GCAGTG	2 cut(s)	616, 643
BtsIMutI	CAGTG	6 cut(s)	139, 384, 616, 643, 1389, 1512
Cac8I	GCNNGC	5 cut(s)	241, 463, 551, 1353, 1491
CciI	TCATGA	1 cut(s)	706
CfoI	GCGC	4 cut(s)	549, 1495, 1524, 1638
Cfr10I	RCCGGY	1 cut(s)	549
Cfr13I	GGNCC	2 cut(s)	467, 1700
CseI	GACGC	1 cut(s)	30
Csp6I	GTAC	6 cut(s)	105, 382, 1010, 1387, 1460, 1661
CviAII	CATG	9 cut(s)	432, 455, 462, 536, 578, 707, 1016, 1246, 1490
CviQI	GTAC	6 cut(s)	105, 382, 1010, 1387, 1460, 1661
DdeI	CTNAG	5 cut(s)	122, 131, 747, 1081, 1540
DpnI	GATC	8 cut(s)	6, 64, 593, 672, 936, 1203, 1371, 1465
DpnII	GATC	8 cut(s)	4, 62, 591, 670, 934, 1201, 1369, 1463
DriI	GACNNNNNGTC	1 cut(s)	22
EaeI	YGGCCR	1 cut(s)	90
Eam1104I	CTCTTC	3 cut(s)	867, 1062, 1608
Eam1105I	GACNNNNNGTC	1 cut(s)	22
EarI	CTCTTC	3 cut(s)	867, 1062, 1608
EciI	GGCGGA	1 cut(s)	1382
Eco57I	CTGAAG	2 cut(s)	1362, 1632
Eco88I	CYCGRG	1 cut(s)	996
EcoRII	CCWGG	2 cut(s)	141, 784
EcoT22I	ATGCAT	2 cut(s)	463, 1199
Esp3I	CGTCTC	1 cut(s)	743
FaeI	CATG	9 cut(s)	435, 458, 465, 539, 581, 710, 1019, 1249, 1493
FalI	AAGNNNNNCTT	6 cut(s)	353, 385, 1335, 1367, 1622, 1654
FaqI	GGGAC	1 cut(s)	1530
FatI	CATG	9 cut(s)	431, 454, 461, 535, 577, 706, 1015, 1245, 1489
FauI	CCCGC	1 cut(s)	1048
FblI	GTMKAC	1 cut(s)	964
Fnu4HI	GCNGC	6 cut(s)	237, 466, 504, 507, 545, 1023
FokI	GGATG	6 cut(s)	635, 770, 1359, 1577, 1694, 1711
Fsp4HI	GCNGC	6 cut(s)	237, 466, 504, 507, 545, 1023
FspBI	CTAG	5 cut(s)	689, 1049, 1134, 1208, 1416
GlaI	GCGC	4 cut(s)	548, 1494, 1523, 1637
GluI	GCNGC	6 cut(s)	237, 466, 504, 507, 545, 1023
GsuI	CTGGAG	2 cut(s)	164, 393
HaeII	RGCGCY	2 cut(s)	550, 1525
HaeIII	GGCC	5 cut(s)	92, 468, 1395, 1702, 1715
HapII	CCGG	2 cut(s)	550, 1123
HgaI	GACGC	1 cut(s)	30
HhaI	GCGC	4 cut(s)	549, 1495, 1524, 1638
Hin1II	CATG	9 cut(s)	435, 458, 465, 539, 581, 710, 1019, 1249, 1493
Hin6I	GCGC	4 cut(s)	547, 1493, 1522, 1636
HinP1I	GCGC	4 cut(s)	547, 1493, 1522, 1636
HincII	GTYRAC	1 cut(s)	1131
HindII	GTYRAC	1 cut(s)	1131
HindIII	AAGCTT	1 cut(s)	1349
HpaII	CCGG	2 cut(s)	550, 1123
HphI	GGTGA	2 cut(s)	191, 1276
Hpy166II	GTNNAC	6 cut(s)	302, 384, 965, 1131, 1243, 1389
Hpy188I	TCNGA	8 cut(s)	123, 322, 670, 841, 880, 1062, 1543, 1740
Hpy8I	GTNNAC	6 cut(s)	302, 384, 965, 1131, 1243, 1389
HpyAV	CCTTC	6 cut(s)	194, 265, 286, 290, 784, 1735
HpyCH4III	ACNGT	5 cut(s)	424, 932, 1009, 1326, 1507
HpyCH4IV	ACGT	2 cut(s)	588, 967
HpyF10VI	GCNNNNNNNGC	3 cut(s)	458, 550, 1352
HpyF3I	CTNAG	5 cut(s)	122, 131, 747, 1081, 1540
HpySE526I	ACGT	2 cut(s)	588, 967
Hsp92II	CATG	9 cut(s)	435, 458, 465, 539, 581, 710, 1019, 1249, 1493
HspAI	GCGC	4 cut(s)	547, 1493, 1522, 1636
KroI	GCCGGC	1 cut(s)	549
KroNI	GCCGGC	1 cut(s)	551
Kzo9I	GATC	8 cut(s)	4, 62, 591, 670, 934, 1201, 1369, 1463
LguI	GCTCTTC	1 cut(s)	1608
LmnI	GCTCC	3 cut(s)	781, 1070, 1645
Lsp1109I	GCAGC	5 cut(s)	223, 490, 493, 556, 1009
LweI	GCATC	9 cut(s)	151, 172, 448, 657, 819, 889, 1209, 1412, 1623
MaeI	CTAG	5 cut(s)	689, 1049, 1134, 1208, 1416
MaeII	ACGT	2 cut(s)	588, 967
MaeIII	GTNAC	3 cut(s)	71, 639, 856
MalI	GATC	8 cut(s)	6, 64, 593, 672, 936, 1203, 1371, 1465
MboI	GATC	8 cut(s)	4, 62, 591, 670, 934, 1201, 1369, 1463
MflI	RGATCY	2 cut(s)	670, 1201
MhlI	GDGCHC	1 cut(s)	454
MlyI	GAGTC	6 cut(s)	123, 127, 637, 830, 884, 1467
MmeI	TCCRAC	1 cut(s)	1400
Mph1103I	ATGCAT	2 cut(s)	463, 1199
MroNI	GCCGGC	1 cut(s)	549
MroXI	GAANNNNTTC	2 cut(s)	327, 531
MseI	TTAA	9 cut(s)	267, 396, 477, 494, 804, 948, 1500, 1650, 1752
MslI	CAYNNNNRTG	1 cut(s)	12
MspA1I	CMGCKG	2 cut(s)	634, 1068
MspCI	CTTAAG	1 cut(s)	395
MspI	CCGG	2 cut(s)	550, 1123
MspR9I	CCNGG	2 cut(s)	143, 786
Mva1269I	GAATGC	1 cut(s)	356
MvaI	CCWGG	2 cut(s)	143, 786
MvnI	CGCG	1 cut(s)	209
MwoI	GCNNNNNNNGC	3 cut(s)	458, 550, 1352
NaeI	GCCGGC	1 cut(s)	551
NdeII	GATC	8 cut(s)	4, 62, 591, 670, 934, 1201, 1369, 1463
NgoMIV	GCCGGC	1 cut(s)	549
NlaIII	CATG	9 cut(s)	435, 458, 465, 539, 581, 710, 1019, 1249, 1493
NlaIV	GGNNCC	5 cut(s)	330, 783, 1038, 1072, 1701
NmeAIII	GCCGAG	1 cut(s)	68
NmuCI	GTSAC	1 cut(s)	639
NsiI	ATGCAT	2 cut(s)	463, 1199
NspI	RCATGY	4 cut(s)	435, 465, 1249, 1493
NspV	TTCGAA	2 cut(s)	287, 988
PaeI	GCATGC	2 cut(s)	465, 1493
PagI	TCATGA	1 cut(s)	706
PciI	ACATGT	2 cut(s)	431, 1245
PciSI	GCTCTTC	1 cut(s)	1608
PcsI	WCGNNNNNNNCGW	1 cut(s)	994
PctI	GAATGC	1 cut(s)	356
PdiI	GCCGGC	1 cut(s)	551
PdmI	GAANNNNTTC	2 cut(s)	327, 531
PfeI	GAWTC	6 cut(s)	284, 598, 743, 761, 819, 881
PkrI	GCNGC	6 cut(s)	238, 467, 505, 508, 546, 1024
PleI	GAGTC	6 cut(s)	123, 126, 636, 830, 883, 1467
PpsI	GAGTC	6 cut(s)	123, 126, 636, 830, 883, 1467
PscI	ACATGT	2 cut(s)	431, 1245
PshBI	ATTAAT	2 cut(s)	477, 494
PsiI	TTATAA	1 cut(s)	1149
Psp6I	CCWGG	2 cut(s)	141, 784
PspGI	CCWGG	2 cut(s)	141, 784
PspN4I	GGNNCC	5 cut(s)	330, 783, 1038, 1072, 1701
PspPI	GGNCC	2 cut(s)	467, 1700
PsrI	GAACNNNNNNTAC	2 cut(s)	365, 397
PsuI	RGATCY	2 cut(s)	670, 1201
PvuII	CAGCTG	1 cut(s)	634
RsaI	GTAC	6 cut(s)	106, 383, 1011, 1388, 1461, 1662
RsaNI	GTAC	6 cut(s)	105, 382, 1010, 1387, 1460, 1661
RseI	CAYNNNNRTG	1 cut(s)	12
SapI	GCTCTTC	1 cut(s)	1608
SaqAI	TTAA	9 cut(s)	267, 396, 477, 494, 804, 948, 1500, 1650, 1752
SatI	GCNGC	6 cut(s)	237, 466, 504, 507, 545, 1023
Sau3AI	GATC	8 cut(s)	4, 62, 591, 670, 934, 1201, 1369, 1463
Sau96I	GGNCC	2 cut(s)	467, 1700
SchI	GAGTC	6 cut(s)	123, 127, 637, 830, 884, 1467
ScrFI	CCNGG	2 cut(s)	143, 786
SduI	GDGCHC	1 cut(s)	454
SfaNI	GCATC	9 cut(s)	151, 172, 448, 657, 819, 889, 1209, 1412, 1623
SfuI	TTCGAA	2 cut(s)	287, 988
SmiMI	CAYNNNNRTG	1 cut(s)	12
SmlI	CTYRAG	2 cut(s)	395, 1595
SmoI	CTYRAG	2 cut(s)	395, 1595
SphI	GCATGC	2 cut(s)	465, 1493
SsiI	CCGC	8 cut(s)	185, 209, 305, 404, 465, 1041, 1068, 1367
SspMI	CTAG	5 cut(s)	689, 1049, 1134, 1208, 1416
StyD4I	CCNGG	2 cut(s)	141, 784
TaaI	ACNGT	5 cut(s)	424, 932, 1009, 1326, 1507
TaiI	ACGT	2 cut(s)	591, 970
TaqI	TCGA	7 cut(s)	56, 287, 567, 741, 988, 1232, 1437
TaqII	GACCGA	1 cut(s)	1203
TatI	WGTACW	4 cut(s)	104, 381, 1009, 1660
TauI	GCSGC	1 cut(s)	468
TfiI	GAWTC	6 cut(s)	284, 598, 743, 761, 819, 881
Tru1I	TTAA	9 cut(s)	267, 396, 477, 494, 804, 948, 1500, 1650, 1752
Tru9I	TTAA	9 cut(s)	267, 396, 477, 494, 804, 948, 1500, 1650, 1752
TscAI	CASTG	6 cut(s)	139, 391, 623, 650, 1396, 1512
TseFI	GTSAC	1 cut(s)	639
TseI	GCWGC	5 cut(s)	236, 503, 506, 544, 1022
Tsp45I	GTSAC	1 cut(s)	639
TspDTI	ATGAA	8 cut(s)	366, 524, 536, 723, 774, 879, 1004, 1461
TspGWI	ACGGA	1 cut(s)	725
TspRI	CASTG	6 cut(s)	139, 391, 623, 650, 1396, 1512
Vha464I	CTTAAG	1 cut(s)	395
VspI	ATTAAT	2 cut(s)	477, 494
XapI	RAATTY	1 cut(s)	1228
XbaI	TCTAGA	2 cut(s)	688, 1207
XceI	RCATGY	4 cut(s)	435, 465, 1249, 1493
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1354
XmiI	GTMKAC	1 cut(s)	964
XmnI	GAANNNNTTC	2 cut(s)	327, 531
XspI	CTAG	5 cut(s)	689, 1049, 1134, 1208, 1416
Zsp2I	ATGCAT	2 cut(s)	463, 1199

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.