Regulatory protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1031 |
| Acc36I | ACCTGC | 1 cut(s) | 1287 |
| AccB1I | GGYRCC | 1 cut(s) | 1604 |
| AccI | GTMKAC | 1 cut(s) | 404 |
| AccII | CGCG | 1 cut(s) | 1636 |
| AccIII | TCCGGA | 1 cut(s) | 987 |
| AciI | CCGC | 2 cut(s) | 477, 1241 |
| AclWI | GGATC | 2 cut(s) | 929, 1518 |
| AcoI | YGGCCR | 1 cut(s) | 1359 |
| AcsI | RAATTY | 2 cut(s) | 991, 1426 |
| AcuI | CTGAAG | 3 cut(s) | 437, 972, 1362 |
| AfaI | GTAC | 3 cut(s) | 1011, 1516, 1662 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 535, 1600 |
| AflII | CTTAAG | 1 cut(s) | 1649 |
| AflIII | ACRYGT | 1 cut(s) | 1634 |
| AgsI | TTSAA | 7 cut(s) | 72, 128, 289, 441, 549, 854, 1411 |
| AjnI | CCWGG | 4 cut(s) | 588, 615, 1299, 1551 |
| Alw21I | GWGCWC | 1 cut(s) | 1075 |
| Alw26I | GTCTC | 2 cut(s) | 1445, 1592 |
| AlwI | GGATC | 2 cut(s) | 929, 1518 |
| Ama87I | CYCGRG | 1 cut(s) | 996 |
| Aor13HI | TCCGGA | 1 cut(s) | 987 |
| AoxI | GGCC | 2 cut(s) | 479, 1359 |
| ApeKI | GCWGC | 8 cut(s) | 166, 169, 349, 785, 920, 923, 1238, 1688 |
| ApoI | RAATTY | 2 cut(s) | 991, 1426 |
| AseI | ATTAAT | 2 cut(s) | 489, 570 |
| AspLEI | GCGC | 1 cut(s) | 1032 |
| AspS9I | GGNCC | 2 cut(s) | 479, 793 |
| AsuHPI | GGTGA | 3 cut(s) | 34, 1112, 1760 |
| AsuII | TTCGAA | 1 cut(s) | 1154 |
| AvaI | CYCGRG | 1 cut(s) | 996 |
| AvaII | GGWCC | 1 cut(s) | 793 |
| AxyI | CCTNAGG | 1 cut(s) | 939 |
| BaeGI | GKGCMC | 2 cut(s) | 466, 1607 |
| BalI | TGGCCA | 1 cut(s) | 1361 |
| BanI | GGYRCC | 1 cut(s) | 1604 |
| BanII | GRGCYC | 2 cut(s) | 1075, 1401 |
| BauI | CACGAG | 1 cut(s) | 1074 |
| BbsI | GAAGAC | 2 cut(s) | 297, 1704 |
| Bbv12I | GWGCWC | 1 cut(s) | 1075 |
| BbvI | GCAGC | 8 cut(s) | 156, 178, 336, 797, 907, 935, 1225, 1675 |
| BccI | CCATC | 5 cut(s) | 97, 1048, 1091, 1228, 1426 |
| BceAI | ACGGC | 2 cut(s) | 191, 578 |
| BciT130I | CCWGG | 4 cut(s) | 590, 617, 1301, 1553 |
| BclI | TGATCA | 1 cut(s) | 652 |
| BcoDI | GTCTC | 2 cut(s) | 1445, 1592 |
| BfaI | CTAG | 4 cut(s) | 120, 186, 1208, 1485 |
| BfmI | CTRYAG | 1 cut(s) | 921 |
| BfrI | CTTAAG | 1 cut(s) | 1649 |
| BfuAI | ACCTGC | 1 cut(s) | 1287 |
| BglII | AGATCT | 1 cut(s) | 682 |
| BisI | GCNGC | 8 cut(s) | 167, 170, 350, 786, 921, 924, 1239, 1689 |
| BlsI | GCNGC | 8 cut(s) | 168, 171, 351, 787, 922, 925, 1240, 1690 |
| BmcAI | AGTACT | 1 cut(s) | 1011 |
| Bme1390I | CCNGG | 4 cut(s) | 590, 617, 1301, 1553 |
| Bme18I | GGWCC | 1 cut(s) | 793 |
| BmeT110I | CYCGRG | 1 cut(s) | 996 |
| BmgT120I | GGNCC | 2 cut(s) | 479, 793 |
| BmiI | GGNNCC | 2 cut(s) | 795, 1606 |
| BmrFI | CCNGG | 4 cut(s) | 590, 617, 1301, 1553 |
| BmrI | ACTGGG | 1 cut(s) | 1431 |
| BmsI | GCATC | 6 cut(s) | 193, 236, 524, 702, 1017, 1234 |
| BmuI | ACTGGG | 1 cut(s) | 1431 |
| BpiI | GAAGAC | 2 cut(s) | 297, 1704 |
| BpmI | CTGGAG | 2 cut(s) | 539, 1322 |
| Bpu10I | CCTNAGC | 1 cut(s) | 392 |
| Bpu14I | TTCGAA | 1 cut(s) | 1154 |
| BpuEI | CTTGAG | 2 cut(s) | 722, 1616 |
| BsaJI | CCNNGG | 4 cut(s) | 429, 588, 1347, 1356 |
| BsaWI | WCCGGW | 2 cut(s) | 987, 1188 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1062, 1092 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 535, 1600 |
| Bse1I | ACTGG | 2 cut(s) | 412, 1426 |
| Bse21I | CCTNAGG | 1 cut(s) | 939 |
| Bse3DI | GCAATG | 4 cut(s) | 780, 1017, 1362, 1537 |
| BseAI | TCCGGA | 1 cut(s) | 987 |
| BseBI | CCWGG | 4 cut(s) | 590, 617, 1301, 1553 |
| BseDI | CCNNGG | 4 cut(s) | 429, 588, 1347, 1356 |
| BseGI | GGATG | 5 cut(s) | 26, 603, 1249, 1441, 1718 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 535, 1600 |
| BseMI | GCAATG | 4 cut(s) | 780, 1017, 1362, 1537 |
| BseMII | CTCAG | 3 cut(s) | 163, 773, 855 |
| BseNI | ACTGG | 2 cut(s) | 412, 1426 |
| BseRI | GAGGAG | 2 cut(s) | 88, 1714 |
| BseSI | GKGCMC | 2 cut(s) | 466, 1607 |
| BseXI | GCAGC | 8 cut(s) | 156, 178, 336, 797, 907, 935, 1225, 1675 |
| BsgI | GTGCAG | 2 cut(s) | 1265, 1484 |
| Bsh1236I | CGCG | 1 cut(s) | 1636 |
| BshFI | GGCC | 2 cut(s) | 481, 1361 |
| BshNI | GGYRCC | 1 cut(s) | 1604 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1075 |
| BsiHKCI | CYCGRG | 1 cut(s) | 996 |
| BsiSI | CCGG | 4 cut(s) | 536, 988, 1123, 1189 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 535, 1600 |
| BsmAI | GTCTC | 2 cut(s) | 1445, 1592 |
| BsmBI | CGTCTC | 1 cut(s) | 1592 |
| BsnI | GGCC | 2 cut(s) | 481, 1361 |
| BsoBI | CYCGRG | 1 cut(s) | 996 |
| Bsp119I | TTCGAA | 1 cut(s) | 1154 |
| Bsp1286I | GDGCHC | 4 cut(s) | 466, 1075, 1401, 1607 |
| Bsp13I | TCCGGA | 1 cut(s) | 987 |
| Bsp143I | GATC | 5 cut(s) | 652, 677, 682, 934, 1523 |
| Bsp19I | CCATGG | 2 cut(s) | 429, 1356 |
| BspACI | CCGC | 2 cut(s) | 477, 1241 |
| BspANI | GGCC | 2 cut(s) | 481, 1361 |
| BspCNI | CTCAG | 3 cut(s) | 162, 772, 854 |
| BspEI | TCCGGA | 1 cut(s) | 987 |
| BspFNI | CGCG | 1 cut(s) | 1636 |
| BspHI | TCATGA | 1 cut(s) | 1500 |
| BspLI | GGNNCC | 2 cut(s) | 795, 1606 |
| BspMAI | CTGCAG | 1 cut(s) | 925 |
| BspMI | ACCTGC | 1 cut(s) | 1287 |
| BspPI | GGATC | 2 cut(s) | 929, 1518 |
| BspT104I | TTCGAA | 1 cut(s) | 1154 |
| BspT107I | GGYRCC | 1 cut(s) | 1604 |
| BspTI | CTTAAG | 1 cut(s) | 1649 |
| BsrDI | GCAATG | 4 cut(s) | 780, 1017, 1362, 1537 |
| BsrI | ACTGG | 2 cut(s) | 412, 1426 |
| BssECI | CCNNGG | 4 cut(s) | 429, 588, 1347, 1356 |
| BssMI | GATC | 5 cut(s) | 652, 677, 682, 934, 1523 |
| BssNAI | GTATAC | 1 cut(s) | 405 |
| BssSI | CACGAG | 1 cut(s) | 1074 |
| BssT1I | CCWWGG | 3 cut(s) | 429, 1347, 1356 |
| Bst1107I | GTATAC | 1 cut(s) | 405 |
| Bst2BI | CACGAG | 1 cut(s) | 1074 |
| Bst2UI | CCWGG | 4 cut(s) | 590, 617, 1301, 1553 |
| Bst4CI | ACNGT | 6 cut(s) | 200, 455, 770, 1009, 1462, 1510 |
| Bst6I | CTCTTC | 1 cut(s) | 1408 |
| BstAFI | CTTAAG | 1 cut(s) | 1649 |
| BstBI | TTCGAA | 1 cut(s) | 1154 |
| BstC8I | GCNNGC | 5 cut(s) | 281, 294, 475, 479, 483 |
| BstDEI | CTNAG | 7 cut(s) | 149, 392, 759, 841, 892, 939, 1692 |
| BstDSI | CCRYGG | 2 cut(s) | 429, 1356 |
| BstF5I | GGATG | 5 cut(s) | 26, 603, 1249, 1441, 1718 |
| BstFNI | CGCG | 1 cut(s) | 1636 |
| BstHHI | GCGC | 1 cut(s) | 1032 |
| BstKTI | GATC | 5 cut(s) | 655, 680, 685, 937, 1526 |
| BstMAI | GTCTC | 2 cut(s) | 1445, 1592 |
| BstMBI | GATC | 5 cut(s) | 652, 677, 682, 934, 1523 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 276, 289, 470, 1036, 1244, 1352 |
| BstNI | CCWGG | 4 cut(s) | 590, 617, 1301, 1553 |
| BstNSI | RCATGY | 2 cut(s) | 477, 1288 |
| BstSCI | CCNGG | 4 cut(s) | 588, 615, 1299, 1551 |
| BstSFI | CTRYAG | 1 cut(s) | 921 |
| BstSLI | GKGCMC | 2 cut(s) | 466, 1607 |
| BstUI | CGCG | 1 cut(s) | 1636 |
| BstV1I | GCAGC | 8 cut(s) | 156, 178, 336, 797, 907, 935, 1225, 1675 |
| BstV2I | GAAGAC | 2 cut(s) | 297, 1704 |
| BstX2I | RGATCY | 2 cut(s) | 682, 1523 |
| BstYI | RGATCY | 2 cut(s) | 682, 1523 |
| BstZ17I | GTATAC | 1 cut(s) | 405 |
| Bsu36I | CCTNAGG | 1 cut(s) | 939 |
| BsuRI | GGCC | 2 cut(s) | 481, 1361 |
| BtgI | CCRYGG | 2 cut(s) | 429, 1356 |
| BtsCI | GGATG | 5 cut(s) | 26, 603, 1249, 1441, 1718 |
| BtsI | GCAGTG | 1 cut(s) | 795 |
| BtsIMutI | CAGTG | 4 cut(s) | 205, 460, 662, 795 |
| BveI | ACCTGC | 1 cut(s) | 1287 |
| Cac8I | GCNNGC | 5 cut(s) | 281, 294, 475, 479, 483 |
| CciI | TCATGA | 1 cut(s) | 1500 |
| CfoI | GCGC | 1 cut(s) | 1032 |
| Cfr13I | GGNCC | 2 cut(s) | 479, 793 |
| CseI | GACGC | 3 cut(s) | 479, 1236, 1642 |
| Csp6I | GTAC | 3 cut(s) | 1010, 1515, 1661 |
| CviAII | CATG | 8 cut(s) | 430, 467, 474, 1285, 1357, 1370, 1501, 1642 |
| CviQI | GTAC | 3 cut(s) | 1010, 1515, 1661 |
| DdeI | CTNAG | 7 cut(s) | 149, 392, 759, 841, 892, 939, 1692 |
| DpnI | GATC | 5 cut(s) | 654, 679, 684, 936, 1525 |
| DpnII | GATC | 5 cut(s) | 652, 677, 682, 934, 1523 |
| DraI | TTTAAA | 1 cut(s) | 1446 |
| EaeI | YGGCCR | 1 cut(s) | 1359 |
| Eam1104I | CTCTTC | 1 cut(s) | 1408 |
| EarI | CTCTTC | 1 cut(s) | 1408 |
| Ecl136II | GAGCTC | 1 cut(s) | 1073 |
| Eco130I | CCWWGG | 3 cut(s) | 429, 1347, 1356 |
| Eco24I | GRGCYC | 2 cut(s) | 1075, 1401 |
| Eco47I | GGWCC | 1 cut(s) | 793 |
| Eco53kI | GAGCTC | 1 cut(s) | 1073 |
| Eco57I | CTGAAG | 3 cut(s) | 437, 972, 1362 |
| Eco81I | CCTNAGG | 1 cut(s) | 939 |
| Eco88I | CYCGRG | 1 cut(s) | 996 |
| EcoICRI | GAGCTC | 1 cut(s) | 1073 |
| EcoRI | GAATTC | 1 cut(s) | 991 |
| EcoRII | CCWGG | 4 cut(s) | 588, 615, 1299, 1551 |
| EcoT14I | CCWWGG | 3 cut(s) | 429, 1347, 1356 |
| EcoT22I | ATGCAT | 3 cut(s) | 251, 517, 1199 |
| EcoT38I | GRGCYC | 2 cut(s) | 1075, 1401 |
| ErhI | CCWWGG | 3 cut(s) | 429, 1347, 1356 |
| Esp3I | CGTCTC | 1 cut(s) | 1592 |
| FaeI | CATG | 8 cut(s) | 433, 470, 477, 1288, 1360, 1373, 1504, 1645 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1685, 1717 |
| FatI | CATG | 8 cut(s) | 429, 466, 473, 1284, 1356, 1369, 1500, 1641 |
| FauI | CCCGC | 1 cut(s) | 470 |
| FbaI | TGATCA | 1 cut(s) | 652 |
| FblI | GTMKAC | 1 cut(s) | 404 |
| Fnu4HI | GCNGC | 8 cut(s) | 167, 170, 350, 786, 921, 924, 1239, 1689 |
| FokI | GGATG | 5 cut(s) | 13, 590, 1256, 1448, 1705 |
| FriOI | GRGCYC | 2 cut(s) | 1075, 1401 |
| Fsp4HI | GCNGC | 8 cut(s) | 167, 170, 350, 786, 921, 924, 1239, 1689 |
| FspBI | CTAG | 4 cut(s) | 120, 186, 1208, 1485 |
| FspI | TGCGCA | 1 cut(s) | 1031 |
| GlaI | GCGC | 1 cut(s) | 1031 |
| GluI | GCNGC | 8 cut(s) | 167, 170, 350, 786, 921, 924, 1239, 1689 |
| GsuI | CTGGAG | 2 cut(s) | 539, 1322 |
| HaeIII | GGCC | 2 cut(s) | 481, 1361 |
| HapII | CCGG | 4 cut(s) | 536, 988, 1123, 1189 |
| HgaI | GACGC | 3 cut(s) | 479, 1236, 1642 |
| HhaI | GCGC | 1 cut(s) | 1032 |
| Hin1II | CATG | 8 cut(s) | 433, 470, 477, 1288, 1360, 1373, 1504, 1645 |
| Hin6I | GCGC | 1 cut(s) | 1030 |
| HinP1I | GCGC | 1 cut(s) | 1030 |
| HincII | GTYRAC | 3 cut(s) | 181, 982, 1131 |
| HindII | GTYRAC | 3 cut(s) | 181, 982, 1131 |
| HindIII | AAGCTT | 1 cut(s) | 375 |
| HinfI | GANTC | 8 cut(s) | 182, 640, 813, 839, 878, 1228, 1453, 1476 |
| HpaI | GTTAAC | 1 cut(s) | 982 |
| HpaII | CCGG | 4 cut(s) | 536, 988, 1123, 1189 |
| HphI | GGTGA | 3 cut(s) | 34, 1112, 1760 |
| Hpy166II | GTNNAC | 8 cut(s) | 181, 241, 323, 405, 451, 793, 982, 1131 |
| Hpy188III | TCNNGA | 9 cut(s) | 261, 686, 701, 716, 817, 988, 1208, 1501, 1580 |
| Hpy8I | GTNNAC | 8 cut(s) | 181, 241, 323, 405, 451, 793, 982, 1131 |
| Hpy99I | CGWCG | 1 cut(s) | 564 |
| HpyAV | CCTTC | 6 cut(s) | 33, 49, 311, 700, 1586, 1753 |
| HpyCH4III | ACNGT | 6 cut(s) | 200, 455, 770, 1009, 1462, 1510 |
| HpyCH4IV | ACGT | 1 cut(s) | 329 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 276, 289, 470, 1036, 1244, 1352 |
| HpyF3I | CTNAG | 7 cut(s) | 149, 392, 759, 841, 892, 939, 1692 |
| HpySE526I | ACGT | 1 cut(s) | 329 |
| Hsp92II | CATG | 8 cut(s) | 433, 470, 477, 1288, 1360, 1373, 1504, 1645 |
| HspAI | GCGC | 1 cut(s) | 1030 |
| Kpn2I | TCCGGA | 1 cut(s) | 987 |
| Ksp22I | TGATCA | 1 cut(s) | 652 |
| KspAI | GTTAAC | 1 cut(s) | 982 |
| Kzo9I | GATC | 5 cut(s) | 652, 677, 682, 934, 1523 |
| LmnI | GCTCC | 3 cut(s) | 163, 1070, 1645 |
| Lsp1109I | GCAGC | 8 cut(s) | 156, 178, 336, 797, 907, 935, 1225, 1675 |
| LweI | GCATC | 6 cut(s) | 193, 236, 524, 702, 1017, 1234 |
| MaeI | CTAG | 4 cut(s) | 120, 186, 1208, 1485 |
| MaeII | ACGT | 1 cut(s) | 329 |
| MaeIII | GTNAC | 2 cut(s) | 770, 946 |
| MalI | GATC | 5 cut(s) | 654, 679, 684, 936, 1525 |
| MboI | GATC | 5 cut(s) | 652, 677, 682, 934, 1523 |
| MboII | GAAGA | 8 cut(s) | 42, 84, 297, 510, 1184, 1248, 1395, 1704 |
| MfeI | CAATTG | 1 cut(s) | 1289 |
| MflI | RGATCY | 2 cut(s) | 682, 1523 |
| MhlI | GDGCHC | 4 cut(s) | 466, 1075, 1401, 1607 |
| MlsI | TGGCCA | 1 cut(s) | 1361 |
| MluI | ACGCGT | 1 cut(s) | 1634 |
| MluNI | TGGCCA | 1 cut(s) | 1361 |
| MlyI | GAGTC | 6 cut(s) | 176, 649, 833, 887, 1447, 1470 |
| MmeI | TCCRAC | 1 cut(s) | 816 |
| Mox20I | TGGCCA | 1 cut(s) | 1361 |
| Mph1103I | ATGCAT | 3 cut(s) | 251, 517, 1199 |
| MroI | TCCGGA | 1 cut(s) | 987 |
| MscI | TGGCCA | 1 cut(s) | 1361 |
| MseI | TTAA | 8 cut(s) | 489, 570, 738, 872, 981, 1445, 1566, 1650 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1022 |
| Msp20I | TGGCCA | 1 cut(s) | 1361 |
| MspA1I | CMGCKG | 2 cut(s) | 17, 169 |
| MspCI | CTTAAG | 1 cut(s) | 1649 |
| MspI | CCGG | 4 cut(s) | 536, 988, 1123, 1189 |
| MspR9I | CCNGG | 4 cut(s) | 590, 617, 1301, 1553 |
| MunI | CAATTG | 1 cut(s) | 1289 |
| MvaI | CCWGG | 4 cut(s) | 590, 617, 1301, 1553 |
| MvnI | CGCG | 1 cut(s) | 1636 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 276, 289, 470, 1036, 1244, 1352 |
| NcoI | CCATGG | 2 cut(s) | 429, 1356 |
| NdeII | GATC | 5 cut(s) | 652, 677, 682, 934, 1523 |
| NlaIII | CATG | 8 cut(s) | 433, 470, 477, 1288, 1360, 1373, 1504, 1645 |
| NlaIV | GGNNCC | 2 cut(s) | 795, 1606 |
| NmuCI | GTSAC | 2 cut(s) | 770, 946 |
| NsbI | TGCGCA | 1 cut(s) | 1031 |
| NsiI | ATGCAT | 3 cut(s) | 251, 517, 1199 |
| NspI | RCATGY | 2 cut(s) | 477, 1288 |
| NspV | TTCGAA | 1 cut(s) | 1154 |
| PaeI | GCATGC | 1 cut(s) | 477 |
| PagI | TCATGA | 1 cut(s) | 1500 |
| PfeI | GAWTC | 2 cut(s) | 813, 1228 |
| PfoI | TCCNGGA | 1 cut(s) | 1551 |
| PkrI | GCNGC | 8 cut(s) | 168, 171, 351, 787, 922, 925, 1240, 1690 |
| PleI | GAGTC | 6 cut(s) | 176, 648, 833, 886, 1447, 1470 |
| PpsI | GAGTC | 6 cut(s) | 176, 648, 833, 886, 1447, 1470 |
| PshBI | ATTAAT | 2 cut(s) | 489, 570 |
| Psp124BI | GAGCTC | 1 cut(s) | 1075 |
| Psp6I | CCWGG | 4 cut(s) | 588, 615, 1299, 1551 |
| PspGI | CCWGG | 4 cut(s) | 588, 615, 1299, 1551 |
| PspN4I | GGNNCC | 2 cut(s) | 795, 1606 |
| PspPI | GGNCC | 2 cut(s) | 479, 793 |
| PstI | CTGCAG | 1 cut(s) | 925 |
| PsuI | RGATCY | 2 cut(s) | 682, 1523 |
| PvuII | CAGCTG | 2 cut(s) | 17, 169 |
| RsaI | GTAC | 3 cut(s) | 1011, 1516, 1662 |
| RsaNI | GTAC | 3 cut(s) | 1010, 1515, 1661 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1022 |
| SacI | GAGCTC | 1 cut(s) | 1075 |
| SaqAI | TTAA | 8 cut(s) | 489, 570, 738, 872, 981, 1445, 1566, 1650 |
| SatI | GCNGC | 8 cut(s) | 167, 170, 350, 786, 921, 924, 1239, 1689 |
| Sau3AI | GATC | 5 cut(s) | 652, 677, 682, 934, 1523 |
| Sau96I | GGNCC | 2 cut(s) | 479, 793 |
| ScaI | AGTACT | 1 cut(s) | 1011 |
| SchI | GAGTC | 6 cut(s) | 176, 649, 833, 887, 1447, 1470 |
| ScrFI | CCNGG | 4 cut(s) | 590, 617, 1301, 1553 |
| SduI | GDGCHC | 4 cut(s) | 466, 1075, 1401, 1607 |
| SfaNI | GCATC | 6 cut(s) | 193, 236, 524, 702, 1017, 1234 |
| SfcI | CTRYAG | 1 cut(s) | 921 |
| SfuI | TTCGAA | 1 cut(s) | 1154 |
| SinI | GGWCC | 1 cut(s) | 793 |
| SmiI | ATTTAAAT | 1 cut(s) | 1446 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1022 |
| SmlI | CTYRAG | 3 cut(s) | 701, 1595, 1649 |
| SmoI | CTYRAG | 3 cut(s) | 701, 1595, 1649 |
| SphI | GCATGC | 1 cut(s) | 477 |
| SsiI | CCGC | 2 cut(s) | 477, 1241 |
| SspMI | CTAG | 4 cut(s) | 120, 186, 1208, 1485 |
| SstI | GAGCTC | 1 cut(s) | 1075 |
| StyD4I | CCNGG | 4 cut(s) | 588, 615, 1299, 1551 |
| StyI | CCWWGG | 3 cut(s) | 429, 1347, 1356 |
| SwaI | ATTTAAAT | 1 cut(s) | 1446 |
| TaaI | ACNGT | 6 cut(s) | 200, 455, 770, 1009, 1462, 1510 |
| TaiI | ACGT | 1 cut(s) | 332 |
| TaqI | TCGA | 1 cut(s) | 1154 |
| TatI | WGTACW | 2 cut(s) | 1009, 1660 |
| TfiI | GAWTC | 2 cut(s) | 813, 1228 |
| Tru1I | TTAA | 8 cut(s) | 489, 570, 738, 872, 981, 1445, 1566, 1650 |
| Tru9I | TTAA | 8 cut(s) | 489, 570, 738, 872, 981, 1445, 1566, 1650 |
| TscAI | CASTG | 4 cut(s) | 205, 460, 662, 795 |
| TseFI | GTSAC | 2 cut(s) | 770, 946 |
| TseI | GCWGC | 8 cut(s) | 166, 169, 349, 785, 920, 923, 1238, 1688 |
| Tsp45I | GTSAC | 2 cut(s) | 770, 946 |
| TspDTI | ATGAA | 8 cut(s) | 15, 387, 912, 1395, 1517, 1552, 1630, 1704 |
| TspGWI | ACGGA | 2 cut(s) | 511, 1469 |
| TspRI | CASTG | 4 cut(s) | 205, 460, 662, 795 |
| Vha464I | CTTAAG | 1 cut(s) | 1649 |
| VpaK11BI | GGWCC | 1 cut(s) | 793 |
| VspI | ATTAAT | 2 cut(s) | 489, 570 |
| XapI | RAATTY | 2 cut(s) | 991, 1426 |
| XbaI | TCTAGA | 1 cut(s) | 1207 |
| XceI | RCATGY | 2 cut(s) | 477, 1288 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1354 |
| XmiI | GTMKAC | 1 cut(s) | 404 |
| XspI | CTAG | 4 cut(s) | 120, 186, 1208, 1485 |
| ZrmI | AGTACT | 1 cut(s) | 1011 |
| Zsp2I | ATGCAT | 3 cut(s) | 251, 517, 1199 |