MD10G1236800.v1.1

Regulatory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000570
Physical Location & Seq
Forward (+)
33319281 .. 33322397
3117 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1236800.v1.1.491

Sequence Viewer

Length: 1755 bp
ATGGCTGATCCATCATCTTCCTTGAGCTTCACCTCATCCTCCCACATATCCAACGGCTCAGTAACCCCCAACTCCTCCTCCCCCGCCGCCAACACCCCGCCGCCCACCCCGGACCTCACCTCCCTGACCAACCTCAGCTCCAGTTTGGGCCGGCTGTTGGCCGACTCCGGCGGCGACTACAGCGACGCCGAAATCACCGTCGAGGGCGTTCCGGTTGCCGTCCACCGATGCATTTTGGCTTCGAGGAGCGAGTTTTTCGCGAAGGTATTCAGCCGGGAGAATGGGGGTTCCGAAAAAGAAGGAAAACCTAGGTACTTTTTGAGTGATTTGCTGCCATTTGGTCATGTGGGTTATGAAGCGTTTGTGGTTTTCTTGGGGTTTGTGTATACTGCAAAGCTGAAGGCTTTTCCGGTCGAGGTTTCGAGCTGCGTTCACATTGTGTGCGGCCATGAAGCTTGTCGACCCGCCATAGATTTTGCTTTGGAGTTGATGTGTGCCTCTTCGGTTTTCGGAATGCCGGAACTTGCCTCAGTACTGCAGCGACAACTTGCTGATTTTGTTGTTAAAGCCGTGGCGGATGATGTTATCCCAATCCTCGTGGTGGCGTTTAATTGTCAGTTAAGTCAGCTCATTGATCAGTGTATAGAGAGAGTGGCACACTCGGATCTTGATAGTATCTCTCTTGAGAAACGACTTCCTGACGAGGTTGTGGCGAAGATTAAAATTCTTCGCCGGAATTCTCAGCACTACTGCGACCCAAACATGCCGATTGTGGACCCCTTGCGTGAAAAGAGAATCAGGAGAATACACAAGGCACTGGACTCCGATGATGTTGAACTTATGAAACTTCTTTTAACCGAGTCTGATGTAACCTTAGATGAAGCCAATGCGCTCCATTATGCTGCAGCCTACTGCGATCCTAAAGTTGTGACTGAAGTGCTTGGTCTGGGTCTGGCTGATGTTAACCTCCGGGATTCCCGGGGTTTTACAGTGCTTCACATTGCTGTGATGCGCAAGGAGCCATCAATTATAATATTGCTTCTGTCTAATGGAGCTCGTGCATCAGAGCCGACCTTAGAAGGTGAGAGCGCTGTTAGTATTTGCCGGAGGTTGACAAGGCCAAAGGATTATCATACAAAAACAGAGCGGGGGCAAGAAGCAAACAAAGACCGAATTTGCATTGATGTTCTGGAGAGAGAAATGCTGAGGAATCCAATGGCTGGAGAGGCATCTATATCTTCCCAAATGACGCCCAATGATCTGCACATGAGATTACTGAAACTGGAGAATCGAGTGACATTTGCCCGATTGTTATTCCCTGCTGAAGCCAAGCTAGCCATGGCGATTGCTCATGCAGACACAACGTCTGAGTTTGCCGGGATCTCTTCAGTGAAAGGATCAAGTGGGAATTTGATGGAGGTTGATTTGAATGAGACTCCCACTGTGCAGAACAAAAGACTCCTTTCTAGGTTGGAATCGCTTGTGAAAACAGTCAGCATGGGTCGATACTATTTTCCACATTGCTCGGAAGTCCTCGATAAGTTGATTCAGGGCGACCTTCCTGATTTGCGCTTCCTTGAGACGGGCACCACCGACGAGCAGAAAATAAAGAGGCAGCGTTTCTTGGAGCTTAAGGAGGAAGTACAAAAGGCATTTGACAAGGACAAAGCTGATAAGAACATCTACGGGTTGTCTCCGTCATCCTCATCGCCTCCGGAACAAGTTGGTGCAAGTCCGAAGGTTAGAAAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008144 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009814 GO:0009816 GO:0009817 GO:0009966 GO:0009987 GO:0010498 GO:0010646 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0023051 GO:0030162 GO:0030163 GO:0031406 GO:0031461 GO:0031625 GO:0032991 GO:0033293 GO:0035821 GO:0036094 GO:0042562 GO:0042742 GO:0042802 GO:0043161 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044003 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051246 GO:0051603 GO:0051701 GO:0051704 GO:0051707 GO:0051817 GO:0052031 GO:0052173 GO:0052200 GO:0052255 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052558 GO:0052559 GO:0052564 GO:0052572 GO:0060255 GO:0065007 GO:0071704 GO:0075136 GO:0080090 GO:0080185 GO:0097159 GO:0098542 GO:1901149 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234 GO:2000022 GO:2000031 GO:2001023 GO:2001038
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 BTB DUF3420 NPR1_like_C

Protein Analysis

585

Amino Acids

64.27

Weight (kDa)

5.86

Isoelectric Point (pI)

46.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 58 - 134 3.7e-08 BTB/POZ domain
DUF3420 PF11900 222 - 270 2.6e-13 Domain of unknown function (DUF3420)
Ank_2 PF12796 270 - 353 3.4e-09 Ankyrin repeats (3 copies)
NPR1_like_C PF12313 369 - 570 1.6e-85 NPR1/NIM1 like defence protein C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000570)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45110 AT5G45110
fragaria_vesca FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_6g38820 FvH4_6g38820 FvH4_6g38821 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830
malus_domestica MD05G1256300.v1.1 MD05G1256400.v1.1 MD09G1146600.v1.1 MD10G1236700.v1.1 MD10G1236800.v1.1 MD17G1133300.v1.1
prunus_persica Prupe.3G178800_v2.0.a1 Prupe.3G178800_v2.0.a1 Prupe.3G178800_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1
pyrus_communis pycom05g23540 pycom05g23550 pycom05g23560 pycom05g23570 pycom09g06690 pycom10g19800 pycom17g12780
rosa_chinensis RchiOBHm_Chr2g0152421 RchiOBHm_Chr2g0152431 RchiOBHm_Chr2g0152441 RchiOBHm_Chr5g0019531
rosa_laevigata RLG00000020632 RLG00000020633 RLG00000032453
rosa_multiflora Rmu_co8414461.1_g000001 Rmu_sc0002759.1_g000050 Rmu_sc0005366.1_g000015 Rmu_sc0005366.1_g000016 Rmu_sc0005366.1_g000017 Rmu_sc0005366.1_g000020 Rmu_sc0005366.1_g000022
rosa_roxburghii Rroxscaffold_1G00058530 Rroxscaffold_2G00095720 Rroxscaffold_2G00095730 Rroxscaffold_2G00095740
rosa_rugosa Rorug02G0432800 Rorug05G0051600
rosa_samantha Rh2AG494300 Rh2AG494400 Rh2AG494500 Rh2BG506600 Rh2BG506700 Rh2CG480600 Rh2CG480700 Rh2DG517700 Rh2DG517800 Rh2DG517900 Rh5AG142400 Rh5BG140800 Rh5CG152900 Rh5DG141500
rosa_wichuraiana Rw2G040670 Rw2G040680 Rw2G040690 Rw5G012600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1031
Acc16I TGCGCA 1 cut(s) 1013
AccB1I GGYRCC 1 cut(s) 1586
AccB7I CCANNNNNTGG 1 cut(s) 1220
AccBSI CCGCTC 1 cut(s) 1147
AccI GTMKAC 2 cut(s) 386, 460
AccII CGCG 1 cut(s) 260
AccIII TCCGGA 1 cut(s) 1714
AciI CCGC 9 cut(s) 84, 87, 98, 101, 171, 444, 465, 575, 1147
AclWI GGATC 5 cut(s) 2, 672, 911, 1388, 1405
AcoI YGGCCR 2 cut(s) 159, 445
AcsI RAATTY 4 cut(s) 723, 736, 1173, 1408
AcuI CTGAAG 4 cut(s) 419, 954, 1344, 1371
AcyI GRCGYC 2 cut(s) 186, 1250
AdeI CACNNNGTG 1 cut(s) 439
AfaI GTAC 3 cut(s) 314, 534, 1644
AfeI AGCGCT 1 cut(s) 1090
AfiI CCNNNNNNNGG 4 cut(s) 157, 601, 1220, 1582
AflII CTTAAG 1 cut(s) 1631
AgsI TTSAA 2 cut(s) 836, 1429
AhdI GACNNNNNGTC 1 cut(s) 1363
Alw21I GWGCWC 1 cut(s) 1057
Alw26I GTCTC 3 cut(s) 1427, 1574, 1698
AlwI GGATC 5 cut(s) 2, 672, 911, 1388, 1405
Ama87I CYCGRG 1 cut(s) 978
Aor13HI TCCGGA 1 cut(s) 1714
Aor51HI AGCGCT 1 cut(s) 1090
AoxI GGCC 4 cut(s) 148, 159, 445, 1118
ApeKI GCWGC 6 cut(s) 331, 426, 538, 902, 905, 1615
ApoI RAATTY 4 cut(s) 723, 736, 1173, 1408
ArsI GACNNNNNNTTYG 2 cut(s) 183, 215
Asp700I GAANNNNTTC 1 cut(s) 266
AspA2I CCTAGG 1 cut(s) 308
AspLEI GCGC 4 cut(s) 892, 1014, 1091, 1572
AspS9I GGNCC 3 cut(s) 112, 148, 775
AsuC2I CCSGG 6 cut(s) 110, 275, 971, 979, 980, 1378
AsuHPI GGTGA 4 cut(s) 22, 109, 187, 1094
AsuNHI GCTAGC 1 cut(s) 1333
AvaI CYCGRG 1 cut(s) 978
AvaII GGWCC 2 cut(s) 112, 775
AvrII CCTAGG 1 cut(s) 308
BaeGI GKGCMC 1 cut(s) 1589
BanI GGYRCC 1 cut(s) 1586
BanII GRGCYC 1 cut(s) 1057
BauI CACGAG 2 cut(s) 596, 1056
Bbv12I GWGCWC 1 cut(s) 1057
BbvCI CCTCAGC 2 cut(s) 134, 1205
BbvI GCAGC 6 cut(s) 318, 413, 550, 889, 917, 1627
BccI CCATC 3 cut(s) 19, 1030, 1408
BceAI ACGGC 3 cut(s) 70, 203, 554
BclI TGATCA 1 cut(s) 634
BcnI CCSGG 6 cut(s) 110, 275, 971, 979, 980, 1378
BcoDI GTCTC 3 cut(s) 1427, 1574, 1698
BfaI CTAG 3 cut(s) 309, 1334, 1467
BfmI CTRYAG 3 cut(s) 178, 536, 903
BfoI RGCGCY 1 cut(s) 1092
BfrI CTTAAG 1 cut(s) 1631
BlnI CCTAGG 1 cut(s) 308
BmcAI AGTACT 1 cut(s) 534
Bme1390I CCNGG 6 cut(s) 110, 275, 971, 979, 980, 1378
Bme18I GGWCC 2 cut(s) 112, 775
BmeRI GACNNNNNGTC 1 cut(s) 1363
BmeT110I CYCGRG 1 cut(s) 978
BmgT120I GGNCC 3 cut(s) 112, 148, 775
BmiI GGNNCC 4 cut(s) 289, 777, 1020, 1588
BmrFI CCNGG 6 cut(s) 110, 275, 971, 979, 980, 1378
BmsI GCATC 4 cut(s) 218, 999, 1070, 1238
BmtI GCTAGC 1 cut(s) 1337
BpmI CTGGAG 4 cut(s) 124, 1211, 1242, 1304
Bpu10I CCTNAGC 2 cut(s) 134, 1205
BpuEI CTTGAG 3 cut(s) 43, 704, 1598
BpuMI CCSGG 6 cut(s) 110, 275, 971, 979, 980, 1378
BsaHI GRCGYC 2 cut(s) 186, 1250
BsaJI CCNNGG 6 cut(s) 108, 308, 570, 978, 979, 1338
BsaWI WCCGGW 3 cut(s) 211, 409, 1714
BsaXI ACNNNNNCTCC 8 cut(s) 62, 92, 104, 122, 134, 152, 476, 506
Bsc4I CCNNNNNNNGG 4 cut(s) 157, 601, 1220, 1582
Bse118I RCCGGY 1 cut(s) 150
Bse1I ACTGG 3 cut(s) 141, 822, 1287
Bse3DI GCAATG 2 cut(s) 999, 1519
BseAI TCCGGA 1 cut(s) 1714
BseDI CCNNGG 6 cut(s) 108, 308, 570, 978, 979, 1338
BseGI GGATG 3 cut(s) 35, 583, 1700
BseLI CCNNNNNNNGG 4 cut(s) 157, 601, 1220, 1582
BseMI GCAATG 2 cut(s) 999, 1519
BseMII CTCAG 6 cut(s) 72, 148, 543, 755, 1196, 1359
BseNI ACTGG 3 cut(s) 141, 822, 1287
BseRI GAGGAG 3 cut(s) 64, 67, 259
BseSI GKGCMC 1 cut(s) 1589
BseXI GCAGC 6 cut(s) 318, 413, 550, 889, 917, 1627
BsgI GTGCAG 2 cut(s) 1247, 1466
Bsh1236I CGCG 1 cut(s) 260
Bsh1285I CGRYCG 1 cut(s) 414
BshFI GGCC 4 cut(s) 150, 161, 447, 1120
BshNI GGYRCC 1 cut(s) 1586
BsiEI CGRYCG 1 cut(s) 414
BsiHKAI GWGCWC 1 cut(s) 1057
BsiHKCI CYCGRG 1 cut(s) 978
BslI CCNNNNNNNGG 4 cut(s) 157, 601, 1220, 1582
BsmAI GTCTC 3 cut(s) 1427, 1574, 1698
BsmBI CGTCTC 1 cut(s) 1574
BsmI GAATGC 1 cut(s) 519
BsnI GGCC 4 cut(s) 150, 161, 447, 1120
BsoBI CYCGRG 1 cut(s) 978
Bsp1286I GDGCHC 2 cut(s) 1057, 1589
Bsp13I TCCGGA 1 cut(s) 1714
Bsp143I GATC 7 cut(s) 7, 634, 664, 916, 1258, 1380, 1397
Bsp19I CCATGG 1 cut(s) 1338
Bsp68I TCGCGA 1 cut(s) 260
BspACI CCGC 9 cut(s) 84, 87, 98, 101, 171, 444, 465, 575, 1147
BspANI GGCC 4 cut(s) 150, 161, 447, 1120
BspCNI CTCAG 6 cut(s) 71, 147, 542, 754, 1197, 1360
BspEI TCCGGA 1 cut(s) 1714
BspFNI CGCG 1 cut(s) 260
BspLI GGNNCC 4 cut(s) 289, 777, 1020, 1588
BspMAI CTGCAG 2 cut(s) 540, 907
BspOI GCTAGC 1 cut(s) 1337
BspPI GGATC 5 cut(s) 2, 672, 911, 1388, 1405
BspT107I GGYRCC 1 cut(s) 1586
BspTI CTTAAG 1 cut(s) 1631
BsrBI CCGCTC 1 cut(s) 1147
BsrDI GCAATG 2 cut(s) 999, 1519
BsrFI RCCGGY 1 cut(s) 150
BsrI ACTGG 3 cut(s) 141, 822, 1287
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 6 cut(s) 108, 308, 570, 978, 979, 1338
BssMI GATC 7 cut(s) 7, 634, 664, 916, 1258, 1380, 1397
BssNAI GTATAC 1 cut(s) 387
BssNI GRCGYC 2 cut(s) 186, 1250
BssSI CACGAG 2 cut(s) 596, 1056
BssT1I CCWWGG 2 cut(s) 308, 1338
Bst1107I GTATAC 1 cut(s) 387
Bst2BI CACGAG 2 cut(s) 596, 1056
Bst4CI ACNGT 4 cut(s) 199, 991, 1444, 1492
Bst6I CTCTTC 2 cut(s) 505, 1390
BstACI GRCGYC 2 cut(s) 186, 1250
BstAFI CTTAAG 1 cut(s) 1631
BstC8I GCNNGC 2 cut(s) 152, 1335
BstDEI CTNAG 8 cut(s) 58, 134, 529, 741, 874, 1075, 1205, 1368
BstDSI CCRYGG 2 cut(s) 570, 1338
BstF5I GGATG 3 cut(s) 35, 583, 1700
BstFNI CGCG 1 cut(s) 260
BstH2I RGCGCY 1 cut(s) 1092
BstHHI GCGC 4 cut(s) 892, 1014, 1091, 1572
BstKTI GATC 7 cut(s) 10, 637, 667, 919, 1261, 1383, 1400
BstMAI GTCTC 3 cut(s) 1427, 1574, 1698
BstMBI GATC 7 cut(s) 7, 634, 664, 916, 1258, 1380, 1397
BstMCI CGRYCG 1 cut(s) 414
BstMWI GCNNNNNNNGC 4 cut(s) 180, 1018, 1226, 1334
BstNSI RCATGY 1 cut(s) 766
BstSCI CCNGG 6 cut(s) 108, 273, 969, 977, 978, 1376
BstSFI CTRYAG 3 cut(s) 178, 536, 903
BstSLI GKGCMC 1 cut(s) 1589
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 6 cut(s) 318, 413, 550, 889, 917, 1627
BstX2I RGATCY 2 cut(s) 664, 1380
BstYI RGATCY 2 cut(s) 664, 1380
BstZ17I GTATAC 1 cut(s) 387
BsuRI GGCC 4 cut(s) 150, 161, 447, 1120
BtgI CCRYGG 2 cut(s) 570, 1338
BtgZI GCGATG 1 cut(s) 1692
BtsCI GGATG 3 cut(s) 35, 583, 1700
BtsIMutI CAGTG 5 cut(s) 644, 815, 996, 1395, 1440
BtuMI TCGCGA 1 cut(s) 260
Cac8I GCNNGC 2 cut(s) 152, 1335
CfoI GCGC 4 cut(s) 892, 1014, 1091, 1572
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 3 cut(s) 112, 148, 775
Cfr9I CCCGGG 1 cut(s) 978
CseI GACGC 2 cut(s) 194, 1258
Csp6I GTAC 3 cut(s) 313, 533, 1643
CspCI CAANNNNNGTGG 2 cut(s) 579, 614
CviAII CATG 7 cut(s) 344, 449, 763, 1267, 1339, 1352, 1498
CviQI GTAC 3 cut(s) 313, 533, 1643
DdeI CTNAG 8 cut(s) 58, 134, 529, 741, 874, 1075, 1205, 1368
DpnI GATC 7 cut(s) 9, 636, 666, 918, 1260, 1382, 1399
DpnII GATC 7 cut(s) 7, 634, 664, 916, 1258, 1380, 1397
DraIII CACNNNGTG 1 cut(s) 439
DriI GACNNNNNGTC 1 cut(s) 1363
EaeI YGGCCR 2 cut(s) 159, 445
Eam1104I CTCTTC 2 cut(s) 505, 1390
Eam1105I GACNNNNNGTC 1 cut(s) 1363
EarI CTCTTC 2 cut(s) 505, 1390
EciI GGCGGA 1 cut(s) 590
Ecl136II GAGCTC 1 cut(s) 1055
Eco130I CCWWGG 2 cut(s) 308, 1338
Eco24I GRGCYC 1 cut(s) 1057
Eco47I GGWCC 2 cut(s) 112, 775
Eco47III AGCGCT 1 cut(s) 1090
Eco53kI GAGCTC 1 cut(s) 1055
Eco57I CTGAAG 4 cut(s) 419, 954, 1344, 1371
Eco88I CYCGRG 1 cut(s) 978
EcoICRI GAGCTC 1 cut(s) 1055
EcoRI GAATTC 1 cut(s) 736
EcoT14I CCWWGG 2 cut(s) 308, 1338
EcoT22I ATGCAT 1 cut(s) 233
EcoT38I GRGCYC 1 cut(s) 1057
ErhI CCWWGG 2 cut(s) 308, 1338
Esp3I CGTCTC 1 cut(s) 1574
FaeI CATG 7 cut(s) 347, 452, 766, 1270, 1342, 1355, 1501
FatI CATG 7 cut(s) 343, 448, 762, 1266, 1338, 1351, 1497
FauI CCCGC 4 cut(s) 91, 105, 472, 1140
FbaI TGATCA 1 cut(s) 634
FblI GTMKAC 2 cut(s) 386, 460
FokI GGATG 3 cut(s) 22, 590, 1687
FriOI GRGCYC 1 cut(s) 1057
FspBI CTAG 3 cut(s) 309, 1334, 1467
FspI TGCGCA 1 cut(s) 1013
GlaI GCGC 4 cut(s) 891, 1013, 1090, 1571
GsuI CTGGAG 4 cut(s) 124, 1211, 1242, 1304
HaeII RGCGCY 1 cut(s) 1092
HaeIII GGCC 4 cut(s) 150, 161, 447, 1120
HgaI GACGC 2 cut(s) 194, 1258
HhaI GCGC 4 cut(s) 892, 1014, 1091, 1572
Hin1I GRCGYC 2 cut(s) 186, 1250
Hin1II CATG 7 cut(s) 347, 452, 766, 1270, 1342, 1355, 1501
Hin6I GCGC 4 cut(s) 890, 1012, 1089, 1570
HinP1I GCGC 4 cut(s) 890, 1012, 1089, 1570
HincII GTYRAC 3 cut(s) 461, 964, 1113
HindII GTYRAC 3 cut(s) 461, 964, 1113
HindIII AAGCTT 1 cut(s) 453
HpaI GTTAAC 1 cut(s) 964
HphI GGTGA 4 cut(s) 22, 109, 187, 1094
Hpy166II GTNNAC 7 cut(s) 223, 387, 433, 461, 775, 964, 1113
Hpy188I TCNGA 9 cut(s) 292, 512, 664, 826, 865, 1066, 1369, 1528, 1737
Hpy188III TCNNGA 8 cut(s) 259, 668, 683, 698, 799, 1190, 1562, 1715
Hpy8I GTNNAC 7 cut(s) 223, 387, 433, 461, 775, 964, 1113
Hpy99I CGWCG 3 cut(s) 188, 203, 1598
HpyAV CCTTC 6 cut(s) 256, 293, 394, 1073, 1568, 1732
HpyCH4III ACNGT 4 cut(s) 199, 991, 1444, 1492
HpyCH4IV ACGT 1 cut(s) 1364
HpyF10VI GCNNNNNNNGC 4 cut(s) 180, 1018, 1226, 1334
HpyF3I CTNAG 8 cut(s) 58, 134, 529, 741, 874, 1075, 1205, 1368
HpySE526I ACGT 1 cut(s) 1364
Hsp92I GRCGYC 2 cut(s) 186, 1250
Hsp92II CATG 7 cut(s) 347, 452, 766, 1270, 1342, 1355, 1501
HspAI GCGC 4 cut(s) 890, 1012, 1089, 1570
Kpn2I TCCGGA 1 cut(s) 1714
KroI GCCGGC 1 cut(s) 150
KroNI GCCGGC 1 cut(s) 152
Ksp22I TGATCA 1 cut(s) 634
KspAI GTTAAC 1 cut(s) 964
Kzo9I GATC 7 cut(s) 7, 634, 664, 916, 1258, 1380, 1397
LmnI GCTCC 6 cut(s) 143, 246, 897, 1018, 1052, 1627
Lsp1109I GCAGC 6 cut(s) 318, 413, 550, 889, 917, 1627
LweI GCATC 4 cut(s) 218, 999, 1070, 1238
MaeI CTAG 3 cut(s) 309, 1334, 1467
MaeII ACGT 1 cut(s) 1364
MaeIII GTNAC 4 cut(s) 61, 868, 928, 1294
MalI GATC 7 cut(s) 9, 636, 666, 918, 1260, 1382, 1399
MbiI CCGCTC 1 cut(s) 1147
MboI GATC 7 cut(s) 7, 634, 664, 916, 1258, 1380, 1397
MboII GAAGA 6 cut(s) 9, 492, 719, 727, 1230, 1377
MflI RGATCY 2 cut(s) 664, 1380
MhlI GDGCHC 2 cut(s) 1057, 1589
MluCI AATT 7 cut(s) 610, 723, 736, 1026, 1173, 1408, 1748
MlyI GAGTC 5 cut(s) 158, 815, 869, 1429, 1452
MmeI TCCRAC 2 cut(s) 75, 1452
Mph1103I ATGCAT 1 cut(s) 233
MroI TCCGGA 1 cut(s) 1714
MroNI GCCGGC 1 cut(s) 150
MroXI GAANNNNTTC 1 cut(s) 266
MseI TTAA 7 cut(s) 564, 609, 620, 720, 854, 963, 1632
MslI CAYNNNNRTG 1 cut(s) 1004
MspCI CTTAAG 1 cut(s) 1631
MspR9I CCNGG 6 cut(s) 110, 275, 971, 979, 980, 1378
Mva1269I GAATGC 1 cut(s) 519
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 4 cut(s) 180, 1018, 1226, 1334
NaeI GCCGGC 1 cut(s) 152
NciI CCSGG 6 cut(s) 110, 275, 971, 979, 980, 1378
NcoI CCATGG 1 cut(s) 1338
NdeII GATC 7 cut(s) 7, 634, 664, 916, 1258, 1380, 1397
NgoMIV GCCGGC 1 cut(s) 150
NheI GCTAGC 1 cut(s) 1333
NlaIII CATG 7 cut(s) 347, 452, 766, 1270, 1342, 1355, 1501
NlaIV GGNNCC 4 cut(s) 289, 777, 1020, 1588
NmuCI GTSAC 2 cut(s) 928, 1294
NruI TCGCGA 1 cut(s) 260
NsbI TGCGCA 1 cut(s) 1013
NsiI ATGCAT 1 cut(s) 233
NspI RCATGY 1 cut(s) 766
PctI GAATGC 1 cut(s) 519
PdiI GCCGGC 1 cut(s) 152
PdmI GAANNNNTTC 1 cut(s) 266
PfeI GAWTC 6 cut(s) 795, 974, 1210, 1288, 1475, 1546
PflMI CCANNNNNTGG 1 cut(s) 1220
PfoI TCCNGGA 1 cut(s) 969
PleI GAGTC 5 cut(s) 158, 815, 868, 1429, 1452
PpsI GAGTC 5 cut(s) 158, 815, 868, 1429, 1452
PsiI TTATAA 1 cut(s) 1031
Psp124BI GAGCTC 1 cut(s) 1057
PspN4I GGNNCC 4 cut(s) 289, 777, 1020, 1588
PspPI GGNCC 3 cut(s) 112, 148, 775
PstI CTGCAG 2 cut(s) 540, 907
PsuI RGATCY 2 cut(s) 664, 1380
RruI TCGCGA 1 cut(s) 260
RsaI GTAC 3 cut(s) 314, 534, 1644
RsaNI GTAC 3 cut(s) 313, 533, 1643
RseI CAYNNNNRTG 1 cut(s) 1004
SacI GAGCTC 1 cut(s) 1057
SalI GTCGAC 1 cut(s) 459
SaqAI TTAA 7 cut(s) 564, 609, 620, 720, 854, 963, 1632
Sau3AI GATC 7 cut(s) 7, 634, 664, 916, 1258, 1380, 1397
Sau96I GGNCC 3 cut(s) 112, 148, 775
ScaI AGTACT 1 cut(s) 534
SchI GAGTC 5 cut(s) 158, 815, 869, 1429, 1452
ScrFI CCNGG 6 cut(s) 110, 275, 971, 979, 980, 1378
SduI GDGCHC 2 cut(s) 1057, 1589
SfaNI GCATC 4 cut(s) 218, 999, 1070, 1238
SfcI CTRYAG 3 cut(s) 178, 536, 903
SinI GGWCC 2 cut(s) 112, 775
SmaI CCCGGG 1 cut(s) 980
SmiMI CAYNNNNRTG 1 cut(s) 1004
SmlI CTYRAG 4 cut(s) 22, 683, 1577, 1631
SmoI CTYRAG 4 cut(s) 22, 683, 1577, 1631
Sse9I AATT 7 cut(s) 610, 723, 736, 1026, 1173, 1408, 1748
SsiI CCGC 9 cut(s) 84, 87, 98, 101, 171, 444, 465, 575, 1147
SspI AATATT 1 cut(s) 1035
SspMI CTAG 3 cut(s) 309, 1334, 1467
SstI GAGCTC 1 cut(s) 1057
StyD4I CCNGG 6 cut(s) 108, 273, 969, 977, 978, 1376
StyI CCWWGG 2 cut(s) 308, 1338
TaaI ACNGT 4 cut(s) 199, 991, 1444, 1492
TaiI ACGT 1 cut(s) 1367
TaqI TCGA 8 cut(s) 201, 242, 414, 422, 460, 1291, 1504, 1536
TaqII GACCGA 1 cut(s) 1185
TasI AATT 7 cut(s) 610, 723, 736, 1026, 1173, 1408, 1748
TatI WGTACW 2 cut(s) 532, 1642
TauI GCSGC 4 cut(s) 89, 103, 174, 447
TfiI GAWTC 6 cut(s) 795, 974, 1210, 1288, 1475, 1546
Tru1I TTAA 7 cut(s) 564, 609, 620, 720, 854, 963, 1632
Tru9I TTAA 7 cut(s) 564, 609, 620, 720, 854, 963, 1632
TscAI CASTG 5 cut(s) 644, 822, 996, 1395, 1447
TseFI GTSAC 2 cut(s) 928, 1294
TseI GCWGC 6 cut(s) 331, 426, 538, 902, 905, 1615
Tsp45I GTSAC 2 cut(s) 928, 1294
TspDTI ATGAA 4 cut(s) 369, 465, 857, 894
TspGWI ACGGA 1 cut(s) 1686
TspMI CCCGGG 1 cut(s) 978
TspRI CASTG 5 cut(s) 644, 822, 996, 1395, 1447
Van91I CCANNNNNTGG 1 cut(s) 1220
Vha464I CTTAAG 1 cut(s) 1631
VpaK11BI GGWCC 2 cut(s) 112, 775
XapI RAATTY 4 cut(s) 723, 736, 1173, 1408
XceI RCATGY 1 cut(s) 766
XcmI CCANNNNNNNNNTGG 1 cut(s) 1336
XmaI CCCGGG 1 cut(s) 978
XmaJI CCTAGG 1 cut(s) 308
XmiI GTMKAC 2 cut(s) 386, 460
XmnI GAANNNNTTC 1 cut(s) 266
XspI CTAG 3 cut(s) 309, 1334, 1467
ZrmI AGTACT 1 cut(s) 534
Zsp2I ATGCAT 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.