Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0000570

Physical Location & Seq

Genomic Coordinates Reverse (-)

66965003 .. 66972277

Gene Structure 7275 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020633

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2256 bp

ATGGATCATTTGAATGACCTTTCGTCATCTTTGAGCTTTGCGTCTTCTTATCTATCGAATGGATCAAGTGGTAACTATGTATCTGCCTCGGCCAATTCTCAAAGTACGGAGCATTTGAGTCTGAGCAGACTCAGTGATAACCTTGAGAGGCTGTTGCTTGATGCTCAATATGACTATAGTGATGCGGATATTGTTGTTGAGGGGGTCGCGGTTGGTGTCAATCGGTGTATATTGGCTGCACGCAGTCAGTTTTTCCATGAGCTTTTTAAGAAAGGGAATGATGATTCGAAGAAGGAAGGTAAACCGCAGTATATGATGTCTGAATTGGTTCCTTTCGGGGGTGGAGTTGGCTATGAAGCATTCAAGGTAGTCTTGAACTACTTGTACACTGGAAAACTTAAGCCACCGCCTCCAGAAGTATCAACTGTATCAACATGTGTTGATGATAATTGTGCCCATGATGCATGCGGCCCTGCTATTAATTACGCTGTGGAATTGATGTATGCTGCTGCCACTTTCCAGATGAAAGAACTTGTTCATGTTGTGCAGCGCCGGCTTACTAACTTTGTCGAGAAAGCTCTCGTTGAAGACGTGATCCCTATTCTTGTAGCCTCTTTCCACTGCAAACAGAGTCAGCTGCTTTCACACTGCATCCAGAGGGTAGCAGGTTCAGATCTTGACAATGTGGTTCTAGAAAAAGAGCTTCCTCATGAAGTTTTTGATATTATCAAATCACTCCGTCTCGAATCTCAGCAAATGGATGAATCAAGTATGCTGGAAATGGAGCCGGGGGAGGAAAAGAGGCTTAAAAGCATCGCAAGAATCCACAAGGCATTAGACTCTGATGATGTTGAACTGGTTACTCTTCTTCTAAAAGAGTCTGGTTCTATTTCATTAGATGATGCTTATGCTCTCCACTATGCTGTTGCATACTGTGATCCAAAAATTGTTAAGGAGGTTCTTGGTCTACGTCTGGCCAATACCAACCTTCGAAATGCCCGAGGACATACTGTACTTCATGTGGCTGCAAGGCGTAAGGAACCCGCAGTCCTAGTTCCTCTTCTGAACAGCGGAGCCTCTGCCTTAGAAACTACATTAGATGGTCAAACTGCTGTTGCAATTTGCCGGAGGTTGACTAGACCAAAGGATTATAATGAGAACACAAAGCAGGGAGAGGTGTCTAACAAAGACCGAATATGCATAGATCTTCTAGAGAGAGAGATGCGAAGAAATTCGATGGCTGTGGACATGTCAAACACATCACAGGTGATAGCTGATGATTTGAATGTGAGGCTGGACTATTTTGAAAATAGAGTGGCATTTGCACGGTTGTTGTTTCCTGCTGAAGCCAAGCTTGCTATGGAAATGGCGGATCATCCAACATCAGGCTACACTGGCCTTTTAGCATCAAAAGGCTCTAGTGGGAACTTACGAGAGGTCGATTTGAATGAAACACCCTCTGTACGATCCAAAAGACTCCATGAAAAATTGCAAGCCCTAATTAAAACAGTGGAAATGGGACGGCGCTTCTTCCCCCATTGCTCAGAAGTTCTTGATAAGTTTCTGGATGATGAGATGGATATGGCTGATTACTTCCTTGAGAAAGGAACTCCTGAAGAGCAGAAAAACAAGAAGATGCGCTTCTTGGAGCTTAAAGATGATGTACAGAAAGCATTTTGCAGGGATGTGGCTGAAAAACAGGGGTCAGTTTTGACGGCCTCATCATCATCCTCATCTTCTCCGAAGGAGGGTGTTAATCCTCAATGTTTCAACAAGTGGGTAATGCTTAGAACTGAGTATTGCCAGCTTCTAGGGTGTTTTTATGCTTCTAGAGGGAGAGAGATGCGAAGAAATTCAATGGCTGTGAACATGTCAAACACATCACAGGTGATAGCTGATGATTTGAATGTGAGGCTGGCCTATTTTGAAAACAGAGTGGCGTTTGCACGGTTGTTGTTTCCTGCGGAAGCCAAGCTTGCTATGGAAATGGCAGATCATCCAACATCAGAGTCTAGTGGGAACTTACGAGAGGTCGATTTGAATGAAACACCCTCTGTACGATCCGAAAGACTCCACGAAAAATTGCAAGCCCTAATTAAAACAGTGGATATGGGACGGCGCTTCTTCCCCCTTTGCTCAGAAGTTCTTGATAAGTTTCTGGATGATGAGACGGACATGGCTGATTACTTCCTTGAAAAAGGAACTCCCGAAGGGGAGAAAAACAAGAGGATGCGCTTCTTGGAACTTAAAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008144 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009814 GO:0009816 GO:0009817 GO:0009966 GO:0009987 GO:0010498 GO:0010646 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0023051 GO:0030162 GO:0030163 GO:0031406 GO:0031461 GO:0031625 GO:0032991 GO:0033293 GO:0035821 GO:0036094 GO:0042562 GO:0042742 GO:0043161 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043207 GO:0043632 GO:0044003 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051246 GO:0051603 GO:0051701 GO:0051704 GO:0051707 GO:0051817 GO:0052031 GO:0052173 GO:0052200 GO:0052255 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052558 GO:0052559 GO:0052564 GO:0052572 GO:0060255 GO:0065007 GO:0071704 GO:0075136 GO:0080090 GO:0080185 GO:0097159 GO:0098542 GO:1901149 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234 GO:2000022

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Ank_2 BTB DUF3420 NPR1_like_C

Protein Analysis

752

Amino Acids

84.32

Weight (kDa)

5.57

Isoelectric Point (pI)

40.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB	PF00651	56 - 144	2.5e-10	BTB/POZ domain
DUF3420	PF11900	225 - 272	1.9e-09	Domain of unknown function (DUF3420)
Ank_2	PF12796	276 - 360	2.2e-06	Ankyrin repeats (3 copies)
NPR1_like_C	PF12313	376 - 578	1e-78	NPR1/NIM1 like defence protein C terminal
NPR1_like_C	PF12313	613 - 750	1.3e-44	NPR1/NIM1 like defence protein C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000570)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45110 AT5G45110
fragaria_vesca	FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_6g38820 FvH4_6g38820 FvH4_6g38821 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830
malus_domestica	MD05G1256300.v1.1 MD05G1256400.v1.1 MD09G1146600.v1.1 MD10G1236700.v1.1 MD10G1236800.v1.1 MD17G1133300.v1.1
prunus_persica	Prupe.3G178800_v2.0.a1 Prupe.3G178800_v2.0.a1 Prupe.3G178800_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1
pyrus_communis	pycom05g23540 pycom05g23550 pycom05g23560 pycom05g23570 pycom09g06690 pycom10g19800 pycom17g12780
rosa_chinensis	RchiOBHm_Chr2g0152421 RchiOBHm_Chr2g0152431 RchiOBHm_Chr2g0152441 RchiOBHm_Chr5g0019531
rosa_laevigata	RLG00000020632 RLG00000020633 RLG00000032453
rosa_multiflora	Rmu_co8414461.1_g000001 Rmu_sc0002759.1_g000050 Rmu_sc0005366.1_g000015 Rmu_sc0005366.1_g000016 Rmu_sc0005366.1_g000017 Rmu_sc0005366.1_g000020 Rmu_sc0005366.1_g000022
rosa_roxburghii	Rroxscaffold_1G00058530 Rroxscaffold_2G00095720 Rroxscaffold_2G00095730 Rroxscaffold_2G00095740
rosa_rugosa	Rorug02G0432800 Rorug05G0051600
rosa_samantha	Rh2AG494300 Rh2AG494400 Rh2AG494500 Rh2BG506600 Rh2BG506700 Rh2CG480600 Rh2CG480700 Rh2DG517700 Rh2DG517800 Rh2DG517900 Rh5AG142400 Rh5BG140800 Rh5CG152900 Rh5DG141500
rosa_wichuraiana	Rw2G040670 Rw2G040680 Rw2G040690 Rw5G012600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1152
Acc36I	ACCTGC	1 cut(s)	656
AccI	GTMKAC	1 cut(s)	967
AccII	CGCG	1 cut(s)	209
AciI	CCGC	9 cut(s)	185, 209, 305, 407, 468, 1044, 1071, 1370, 1964
AclWI	GGATC	7 cut(s)	12, 70, 589, 932, 1380, 1461, 2055
AcoI	YGGCCR	2 cut(s)	90, 975
AcsI	RAATTY	2 cut(s)	1231, 1852
AcuI	CTGAAG	2 cut(s)	1365, 1635
AfaI	GTAC	6 cut(s)	106, 386, 1014, 1464, 1665, 2058
AfiI	CCNNNNNNNGG	3 cut(s)	338, 1385, 1748
AflII	CTTAAG	1 cut(s)	398
AflIII	ACRYGT	3 cut(s)	434, 1248, 1869
AhdI	GACNNNNNGTC	1 cut(s)	22
AjiI	CACGTC	1 cut(s)	592
AloI	GAACNNNNNNTCC	2 cut(s)	1032, 1064
Alw26I	GTCTC	2 cut(s)	746, 2162
AlwI	GGATC	7 cut(s)	12, 70, 589, 932, 1380, 1461, 2055
Ama87I	CYCGRG	1 cut(s)	999
AoxI	GGCC	6 cut(s)	90, 469, 975, 1396, 1716, 1917
ApeKI	GCWGC	6 cut(s)	236, 506, 509, 547, 637, 1025
ApoI	RAATTY	2 cut(s)	1231, 1852
AseI	ATTAAT	1 cut(s)	480
Asp700I	GAANNNNTTC	4 cut(s)	327, 534, 1231, 1852
AspLEI	GCGC	5 cut(s)	552, 1527, 1641, 2121, 2235
AspS9I	GGNCC	1 cut(s)	470
AsuC2I	CCSGG	1 cut(s)	789
AsuHPI	GGTGA	2 cut(s)	1279, 1900
AsuII	TTCGAA	2 cut(s)	287, 991
AvaI	CYCGRG	1 cut(s)	999
BaeGI	GKGCMC	1 cut(s)	457
BalI	TGGCCA	1 cut(s)	977
BbsI	GAAGAC	2 cut(s)	36, 594
BbvI	GCAGC	6 cut(s)	223, 493, 496, 559, 624, 1012
BccI	CCATC	3 cut(s)	1094, 1231, 1570
BceAI	ACGGC	3 cut(s)	1538, 1731, 2132
BcnI	CCSGG	1 cut(s)	789
BcoDI	GTCTC	2 cut(s)	746, 2162
BfaI	CTAG	8 cut(s)	692, 1052, 1137, 1211, 1419, 1811, 1830, 2013
BfmI	CTRYAG	1 cut(s)	175
BfoI	RGCGCY	3 cut(s)	553, 1528, 2122
BfrI	CTTAAG	1 cut(s)	398
BfuAI	ACCTGC	1 cut(s)	656
BglII	AGATCT	2 cut(s)	673, 1204
BisI	GCNGC	7 cut(s)	237, 469, 507, 510, 548, 638, 1026
BlsI	GCNGC	7 cut(s)	238, 470, 508, 511, 549, 639, 1027
Bme1390I	CCNGG	1 cut(s)	789
BmeRI	GACNNNNNGTC	1 cut(s)	22
BmeT110I	CYCGRG	1 cut(s)	999
BmgBI	CACGTC	1 cut(s)	592
BmgT120I	GGNCC	1 cut(s)	470
BmiI	GGNNCC	4 cut(s)	330, 786, 1041, 1075
BmrFI	CCNGG	1 cut(s)	789
BpiI	GAAGAC	2 cut(s)	36, 594
BpmI	CTGGAG	1 cut(s)	396
Bpu14I	TTCGAA	2 cut(s)	287, 991
BpuEI	CTTGAG	2 cut(s)	164, 1619
BpuMI	CCSGG	1 cut(s)	789
BsaJI	CCNNGG	3 cut(s)	87, 788, 1000
Bsc4I	CCNNNNNNNGG	3 cut(s)	338, 1385, 1748
Bse118I	RCCGGY	1 cut(s)	552
Bse1I	ACTGG	3 cut(s)	394, 861, 1399
Bse3DI	GCAATG	1 cut(s)	1537
BseDI	CCNNGG	3 cut(s)	87, 788, 1000
BseGI	GGATG	9 cut(s)	651, 766, 1375, 1573, 1690, 1727, 1996, 2167, 2235
BseLI	CCNNNNNNNGG	3 cut(s)	338, 1385, 1748
BseMI	GCAATG	1 cut(s)	1537
BseMII	CTCAG	6 cut(s)	113, 145, 764, 1557, 1785, 2151
BseNI	ACTGG	3 cut(s)	394, 861, 1399
BseSI	GKGCMC	1 cut(s)	457
BseXI	GCAGC	6 cut(s)	223, 493, 496, 559, 624, 1012
BsgI	GTGCAG	2 cut(s)	222, 566
Bsh1236I	CGCG	1 cut(s)	209
BshFI	GGCC	6 cut(s)	92, 471, 977, 1398, 1718, 1919
BsiHKCI	CYCGRG	1 cut(s)	999
BsiSI	CCGG	3 cut(s)	553, 788, 1126
BslFI	GGGAC	2 cut(s)	1533, 2127
BslI	CCNNNNNNNGG	3 cut(s)	338, 1385, 1748
BsmAI	GTCTC	2 cut(s)	746, 2162
BsmBI	CGTCTC	2 cut(s)	746, 2162
BsmFI	GGGAC	2 cut(s)	1533, 2127
BsmI	GAATGC	1 cut(s)	359
BsnI	GGCC	6 cut(s)	92, 471, 977, 1398, 1718, 1919
BsoBI	CYCGRG	1 cut(s)	999
Bsp119I	TTCGAA	2 cut(s)	287, 991
Bsp1286I	GDGCHC	1 cut(s)	457
Bsp1407I	TGTACA	2 cut(s)	384, 1663
BspACI	CCGC	9 cut(s)	185, 209, 305, 407, 468, 1044, 1071, 1370, 1964
BspANI	GGCC	6 cut(s)	92, 471, 977, 1398, 1718, 1919
BspCNI	CTCAG	6 cut(s)	114, 144, 763, 1556, 1786, 2150
BspFNI	CGCG	1 cut(s)	209
BspHI	TCATGA	1 cut(s)	709
BspLI	GGNNCC	4 cut(s)	330, 786, 1041, 1075
BspMI	ACCTGC	1 cut(s)	656
BspPI	GGATC	7 cut(s)	12, 70, 589, 932, 1380, 1461, 2055
BspQI	GCTCTTC	1 cut(s)	1611
BspT104I	TTCGAA	2 cut(s)	287, 991
BspTI	CTTAAG	1 cut(s)	398
BsrDI	GCAATG	1 cut(s)	1537
BsrFI	RCCGGY	1 cut(s)	552
BsrGI	TGTACA	2 cut(s)	384, 1663
BsrI	ACTGG	3 cut(s)	394, 861, 1399
BssAI	RCCGGY	1 cut(s)	552
BssECI	CCNNGG	3 cut(s)	87, 788, 1000
Bst4CI	ACNGT	7 cut(s)	427, 935, 1012, 1329, 1510, 1950, 2104
Bst6I	CTCTTC	3 cut(s)	870, 1065, 1611
BstAFI	CTTAAG	1 cut(s)	398
BstAUI	TGTACA	2 cut(s)	384, 1663
BstBI	TTCGAA	2 cut(s)	287, 991
BstC8I	GCNNGC	9 cut(s)	241, 466, 554, 1356, 1494, 1805, 1917, 1977, 2088
BstDEI	CTNAG	8 cut(s)	122, 131, 750, 1084, 1543, 1787, 1794, 2137
BstF5I	GGATG	9 cut(s)	651, 766, 1375, 1573, 1690, 1727, 1996, 2167, 2235
BstFNI	CGCG	1 cut(s)	209
BstH2I	RGCGCY	3 cut(s)	553, 1528, 2122
BstHHI	GCGC	5 cut(s)	552, 1527, 1641, 2121, 2235
BstMAI	GTCTC	2 cut(s)	746, 2162
BstMWI	GCNNNNNNNGC	5 cut(s)	461, 553, 1355, 1395, 1976
BstNSI	RCATGY	4 cut(s)	438, 468, 1252, 1873
BstSCI	CCNGG	1 cut(s)	787
BstSFI	CTRYAG	1 cut(s)	175
BstSLI	GKGCMC	1 cut(s)	457
BstUI	CGCG	1 cut(s)	209
BstV1I	GCAGC	6 cut(s)	223, 493, 496, 559, 624, 1012
BstV2I	GAAGAC	2 cut(s)	36, 594
BstX2I	RGATCY	2 cut(s)	673, 1204
BstYI	RGATCY	2 cut(s)	673, 1204
BsuRI	GGCC	6 cut(s)	92, 471, 977, 1398, 1718, 1919
BtgZI	GCGATG	1 cut(s)	799
BtrI	CACGTC	1 cut(s)	592
BtsCI	GGATG	9 cut(s)	651, 766, 1375, 1573, 1690, 1727, 1996, 2167, 2235
BtsI	GCAGTG	2 cut(s)	619, 646
BtsIMutI	CAGTG	7 cut(s)	139, 387, 619, 646, 1392, 1515, 2109
BveI	ACCTGC	1 cut(s)	656
Cac8I	GCNNGC	9 cut(s)	241, 466, 554, 1356, 1494, 1805, 1917, 1977, 2088
CciI	TCATGA	1 cut(s)	709
CfoI	GCGC	5 cut(s)	552, 1527, 1641, 2121, 2235
Cfr10I	RCCGGY	1 cut(s)	552
Cfr13I	GGNCC	1 cut(s)	470
CseI	GACGC	1 cut(s)	30
Csp6I	GTAC	6 cut(s)	105, 385, 1013, 1463, 1664, 2057
CviQI	GTAC	6 cut(s)	105, 385, 1013, 1463, 1664, 2057
DdeI	CTNAG	8 cut(s)	122, 131, 750, 1084, 1543, 1787, 1794, 2137
DriI	GACNNNNNGTC	1 cut(s)	22
EaeI	YGGCCR	2 cut(s)	90, 975
Eam1104I	CTCTTC	3 cut(s)	870, 1065, 1611
Eam1105I	GACNNNNNGTC	1 cut(s)	22
EarI	CTCTTC	3 cut(s)	870, 1065, 1611
EciI	GGCGGA	1 cut(s)	1385
Eco57I	CTGAAG	2 cut(s)	1365, 1635
Eco88I	CYCGRG	1 cut(s)	999
EcoT22I	ATGCAT	2 cut(s)	466, 1202
Esp3I	CGTCTC	2 cut(s)	746, 2162
FalI	AAGNNNNNCTT	8 cut(s)	356, 388, 1338, 1370, 1625, 1657, 1959, 1991
FaqI	GGGAC	2 cut(s)	1533, 2127
FauI	CCCGC	1 cut(s)	1051
FblI	GTMKAC	1 cut(s)	967
Fnu4HI	GCNGC	7 cut(s)	237, 469, 507, 510, 548, 638, 1026
FokI	GGATG	9 cut(s)	638, 773, 1362, 1580, 1697, 1714, 1983, 2174, 2242
Fsp4HI	GCNGC	7 cut(s)	237, 469, 507, 510, 548, 638, 1026
FspBI	CTAG	8 cut(s)	692, 1052, 1137, 1211, 1419, 1811, 1830, 2013
GlaI	GCGC	5 cut(s)	551, 1526, 1640, 2120, 2234
GluI	GCNGC	7 cut(s)	237, 469, 507, 510, 548, 638, 1026
GsuI	CTGGAG	1 cut(s)	396
HaeII	RGCGCY	3 cut(s)	553, 1528, 2122
HaeIII	GGCC	6 cut(s)	92, 471, 977, 1398, 1718, 1919
HapII	CCGG	3 cut(s)	553, 788, 1126
HgaI	GACGC	1 cut(s)	30
HhaI	GCGC	5 cut(s)	552, 1527, 1641, 2121, 2235
Hin6I	GCGC	5 cut(s)	550, 1525, 1639, 2119, 2233
HinP1I	GCGC	5 cut(s)	550, 1525, 1639, 2119, 2233
HincII	GTYRAC	1 cut(s)	1134
HindII	GTYRAC	1 cut(s)	1134
HindIII	AAGCTT	2 cut(s)	1352, 1973
HpaII	CCGG	3 cut(s)	553, 788, 1126
HphI	GGTGA	2 cut(s)	1279, 1900
Hpy166II	GTNNAC	6 cut(s)	302, 387, 968, 1134, 1246, 1867
Hpy8I	GTNNAC	6 cut(s)	302, 387, 968, 1134, 1246, 1867
HpyAV	CCTTC	5 cut(s)	286, 290, 998, 1738, 2204
HpyCH4III	ACNGT	7 cut(s)	427, 935, 1012, 1329, 1510, 1950, 2104
HpyCH4IV	ACGT	2 cut(s)	591, 970
HpyF10VI	GCNNNNNNNGC	5 cut(s)	461, 553, 1355, 1395, 1976
HpyF3I	CTNAG	8 cut(s)	122, 131, 750, 1084, 1543, 1787, 1794, 2137
HpySE526I	ACGT	2 cut(s)	591, 970
HspAI	GCGC	5 cut(s)	550, 1525, 1639, 2119, 2233
KroI	GCCGGC	1 cut(s)	552
KroNI	GCCGGC	1 cut(s)	554
LguI	GCTCTTC	1 cut(s)	1611
LmnI	GCTCC	4 cut(s)	109, 784, 1073, 1648
Lsp1109I	GCAGC	6 cut(s)	223, 493, 496, 559, 624, 1012
MaeI	CTAG	8 cut(s)	692, 1052, 1137, 1211, 1419, 1811, 1830, 2013
MaeII	ACGT	2 cut(s)	591, 970
MaeIII	GTNAC	2 cut(s)	71, 859
MflI	RGATCY	2 cut(s)	673, 1204
MhlI	GDGCHC	1 cut(s)	457
MlsI	TGGCCA	1 cut(s)	977
MluNI	TGGCCA	1 cut(s)	977
MlyI	GAGTC	8 cut(s)	123, 127, 640, 833, 887, 1470, 2018, 2064
MmeI	TCCRAC	2 cut(s)	1403, 2024
Mox20I	TGGCCA	1 cut(s)	977
Mph1103I	ATGCAT	2 cut(s)	466, 1202
MroNI	GCCGGC	1 cut(s)	552
MroXI	GAANNNNTTC	4 cut(s)	327, 534, 1231, 1852
MscI	TGGCCA	1 cut(s)	977
MslI	CAYNNNNRTG	1 cut(s)	12
Msp20I	TGGCCA	1 cut(s)	977
MspA1I	CMGCKG	2 cut(s)	637, 1071
MspCI	CTTAAG	1 cut(s)	398
MspI	CCGG	3 cut(s)	553, 788, 1126
MspR9I	CCNGG	1 cut(s)	789
Mva1269I	GAATGC	1 cut(s)	359
MvnI	CGCG	1 cut(s)	209
MwoI	GCNNNNNNNGC	5 cut(s)	461, 553, 1355, 1395, 1976
NaeI	GCCGGC	1 cut(s)	554
NciI	CCSGG	1 cut(s)	789
NgoMIV	GCCGGC	1 cut(s)	552
NlaIV	GGNNCC	4 cut(s)	330, 786, 1041, 1075
NmeAIII	GCCGAG	1 cut(s)	68
NsiI	ATGCAT	2 cut(s)	466, 1202
NspI	RCATGY	4 cut(s)	438, 468, 1252, 1873
NspV	TTCGAA	2 cut(s)	287, 991
PaeI	GCATGC	1 cut(s)	468
PagI	TCATGA	1 cut(s)	709
PciI	ACATGT	3 cut(s)	434, 1248, 1869
PciSI	GCTCTTC	1 cut(s)	1611
PcsI	WCGNNNNNNNCGW	2 cut(s)	588, 997
PctI	GAATGC	1 cut(s)	359
PdiI	GCCGGC	1 cut(s)	554
PdmI	GAANNNNTTC	4 cut(s)	327, 534, 1231, 1852
PfeI	GAWTC	4 cut(s)	284, 746, 764, 822
PkrI	GCNGC	7 cut(s)	238, 470, 508, 511, 549, 639, 1027
PleI	GAGTC	8 cut(s)	123, 126, 639, 833, 886, 1470, 2017, 2064
PpsI	GAGTC	8 cut(s)	123, 126, 639, 833, 886, 1470, 2017, 2064
PscI	ACATGT	3 cut(s)	434, 1248, 1869
PshBI	ATTAAT	1 cut(s)	480
PsiI	TTATAA	1 cut(s)	1152
PspN4I	GGNNCC	4 cut(s)	330, 786, 1041, 1075
PspPI	GGNCC	1 cut(s)	470
PsrI	GAACNNNNNNTAC	2 cut(s)	368, 400
PsuI	RGATCY	2 cut(s)	673, 1204
PvuII	CAGCTG	1 cut(s)	637
RsaI	GTAC	6 cut(s)	106, 386, 1014, 1464, 1665, 2058
RsaNI	GTAC	6 cut(s)	105, 385, 1013, 1463, 1664, 2057
RseI	CAYNNNNRTG	1 cut(s)	12
SapI	GCTCTTC	1 cut(s)	1611
SatI	GCNGC	7 cut(s)	237, 469, 507, 510, 548, 638, 1026
Sau96I	GGNCC	1 cut(s)	470
SchI	GAGTC	8 cut(s)	123, 127, 640, 833, 887, 1470, 2018, 2064
ScrFI	CCNGG	1 cut(s)	789
SduI	GDGCHC	1 cut(s)	457
SfcI	CTRYAG	1 cut(s)	175
SfuI	TTCGAA	2 cut(s)	287, 991
SmiMI	CAYNNNNRTG	1 cut(s)	12
SmlI	CTYRAG	3 cut(s)	143, 398, 1598
SmoI	CTYRAG	3 cut(s)	143, 398, 1598
SphI	GCATGC	1 cut(s)	468
SsiI	CCGC	9 cut(s)	185, 209, 305, 407, 468, 1044, 1071, 1370, 1964
SspMI	CTAG	8 cut(s)	692, 1052, 1137, 1211, 1419, 1811, 1830, 2013
StyD4I	CCNGG	1 cut(s)	787
TaaI	ACNGT	7 cut(s)	427, 935, 1012, 1329, 1510, 1950, 2104
TaiI	ACGT	2 cut(s)	594, 973
TaqI	TCGA	8 cut(s)	56, 287, 570, 744, 991, 1235, 1440, 2034
TaqII	GACCGA	1 cut(s)	1206
TatI	WGTACW	3 cut(s)	384, 1012, 1663
TauI	GCSGC	1 cut(s)	471
TfiI	GAWTC	4 cut(s)	284, 746, 764, 822
TscAI	CASTG	7 cut(s)	139, 394, 626, 653, 1399, 1515, 2109
TseI	GCWGC	6 cut(s)	236, 506, 509, 547, 637, 1025
TspGWI	ACGGA	3 cut(s)	122, 728, 2186
TspRI	CASTG	7 cut(s)	139, 394, 626, 653, 1399, 1515, 2109
Vha464I	CTTAAG	1 cut(s)	398
VspI	ATTAAT	1 cut(s)	480
XapI	RAATTY	2 cut(s)	1231, 1852
XbaI	TCTAGA	3 cut(s)	691, 1210, 1829
XceI	RCATGY	4 cut(s)	438, 468, 1252, 1873
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1357, 1978
XmiI	GTMKAC	1 cut(s)	967
XmnI	GAANNNNTTC	4 cut(s)	327, 534, 1231, 1852
XspI	CTAG	8 cut(s)	692, 1052, 1137, 1211, 1419, 1811, 1830, 2013
Zsp2I	ATGCAT	2 cut(s)	466, 1202

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.