MD05G1256400.v1.1

Regulatory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000570
Physical Location & Seq
Forward (+)
38749838 .. 38752938
3101 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1256400.v1.1.491

Sequence Viewer

Length: 1743 bp
ATGGCTAATTCATCCGACCCATCTTCCTGCTTGAGCTTCACCTCCTCTCCCCACATATCCAACGCCTCCATAACCCCCAACTCCTCCGCCTCCGACCCGCCGCCCACCCCCGACTTACTCACCAACCTCAGCTCCAATTTGGGCCGGCTACTGGCCGACTCCGGCGGCGACTACAGCGACGCCGAAATCACCGTCGAGGGCGTTCCGGTTGCTGTCCACCGATGTATTTTGGCTTCGAGGAGCGAGTTTTTCGCGGAGTTATTCAGGCGGGAAAATGGGGATTCCGAACAAGAAGGAAAGCCCAAGTATTGTATGAGTGATTTGCTGCCTTTTGGGCATGTGGGTTACGAAGCGTTTCTGGTTTTCTTGAGCTTTGTGTATACGGCAAAGGTGAGGGATTTTCCGGTCGAGGTTTCGAGCTGCGTTCACAATGTGTGCGGCCATGAAGCTTGTCGACCCGCCATTGAATTTTCTTTGGAGTTGATGTGTGCTTCTTCGGTTTTCGGAATGCCGGATCTGGTTTCCATATTGCAGCGACAGCTTATCGATTTTGTACTGAAAGCTCTGGCGGATGATGTTATCCCAATCCTCGTGGTTGCGTTTCATTGTCAGTTAAGTCAGCTCATTGATCAGTGTATAGAGAGAGTGGCACACTCAGATCTTGATAGTATATCTCTAGAGAAAGCACTTCCTGATGAGGTTGTGGAGAAGATTAAAATTCTTCGCCGCAATTCTCAGCACTGCTGCGACCCAAACATGCCAGCCGTGGACCCCTTGCGTGAGAAGAGAATCAGGAGAATACATAAGGCACTGGACTCAGATGATGTTGAACTTATGAAACTTCTTTTAACCGAGTCTGATGTAACCTTAGACGAAGCCAATGCGCTTCATTATGCTGCTGCTTACTGCGATCCTAAGGTTGTGGCTGAAGTGCTTGGCCTGGGTCTGGCTGATGTTAACCTCCGGGATTCCCGGGGTTATACAGTGCTTCACATTGCTGTGAAGCGGAAAGAGCCATCAATTATAATATTGCTTTTGTCTAATGGTGCTCGTGCATCAGAGCCGACTTTAGAAGGTGAGAGTGCTGTTAGTATTTGCCGGAGGTTGACAAGGCAAAAGGATTATCATACAAAAACTGAGCGGGGGCAAGAAGCAAACAAAGACCGGATTTGCATTGATGTTCTAGAGAGAGAGATGCAGAGGAATCCAATGGCTGGAGAGGCATCTATATCTTCCCAAATAACGCCCAATGATCTGCACATGAGACTGCTGAAACTGGAGAATCGAGTGGCATTTGCCAGATTGTTATTCCCTGCTGAAGCCAAGCTAGCCATGGCCATTTCACAGGCAGACACAACGCCTGAGTTTGCCGGGATCTCTTCCTTGAAAGGATCTAGTGGGAATTTGATGGAGGTTGATTTGAACGAGACTCCCACTGTGCAGAACAAAAGACTCCTTTCTAGGTTGGAATCCCTTGTGAAAACAGTCAGTATGGGTAGACACTATTTTCCGCATTGCTCGGAAGTCCTCGATAAGTTCCTTCAGGTCGACCTTCCTGATTTGTGTGTCCTCGAGACGGGCACCACTGACGAGCAGAAAATAAAGAGGCAGCGTTTCTTGGAGCTCAAGGAAGAAGTACAAAAGGCATTTGACAAGGACAAAGCTGATAAGAACCTCTCCGGGCTGTCTTCGTCATCCTCCTCACTTCCTCCGAAAAAAGTTGGGCGCAAGTCCAAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008144 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009814 GO:0009816 GO:0009817 GO:0009966 GO:0009987 GO:0010498 GO:0010646 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0023051 GO:0030162 GO:0030163 GO:0031406 GO:0031461 GO:0031625 GO:0032991 GO:0033293 GO:0035821 GO:0036094 GO:0042562 GO:0042742 GO:0042802 GO:0043161 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044003 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051246 GO:0051603 GO:0051701 GO:0051704 GO:0051707 GO:0051817 GO:0052031 GO:0052173 GO:0052200 GO:0052255 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052558 GO:0052559 GO:0052564 GO:0052572 GO:0060255 GO:0065007 GO:0071704 GO:0075136 GO:0080090 GO:0080185 GO:0097159 GO:0098542 GO:1901149 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234 GO:2000022 GO:2000031 GO:2001023 GO:2001038
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 BTB DUF3420 NPR1_like_C

Protein Analysis

581

Amino Acids

64.06

Weight (kDa)

5.63

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 56 - 132 3.9e-09 BTB/POZ domain
DUF3420 PF11900 220 - 268 1.1e-12 Domain of unknown function (DUF3420)
Ank_2 PF12796 268 - 351 1.8e-09 Ankyrin repeats (3 copies)
NPR1_like_C PF12313 367 - 568 2.3e-87 NPR1/NIM1 like defence protein C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000570)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45110 AT5G45110
fragaria_vesca FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_3g11950 FvH4_6g38820 FvH4_6g38820 FvH4_6g38821 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830 FvH4_6g38830
malus_domestica MD05G1256300.v1.1 MD05G1256400.v1.1 MD09G1146600.v1.1 MD10G1236700.v1.1 MD10G1236800.v1.1 MD17G1133300.v1.1
prunus_persica Prupe.3G178800_v2.0.a1 Prupe.3G178800_v2.0.a1 Prupe.3G178800_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107500_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1 Prupe.4G107800_v2.0.a1
pyrus_communis pycom05g23540 pycom05g23550 pycom05g23560 pycom05g23570 pycom09g06690 pycom10g19800 pycom17g12780
rosa_chinensis RchiOBHm_Chr2g0152421 RchiOBHm_Chr2g0152431 RchiOBHm_Chr2g0152441 RchiOBHm_Chr5g0019531
rosa_laevigata RLG00000020632 RLG00000020633 RLG00000032453
rosa_multiflora Rmu_co8414461.1_g000001 Rmu_sc0002759.1_g000050 Rmu_sc0005366.1_g000015 Rmu_sc0005366.1_g000016 Rmu_sc0005366.1_g000017 Rmu_sc0005366.1_g000020 Rmu_sc0005366.1_g000022
rosa_roxburghii Rroxscaffold_1G00058530 Rroxscaffold_2G00095720 Rroxscaffold_2G00095730 Rroxscaffold_2G00095740
rosa_rugosa Rorug02G0432800 Rorug05G0051600
rosa_samantha Rh2AG494300 Rh2AG494400 Rh2AG494500 Rh2BG506600 Rh2BG506700 Rh2CG480600 Rh2CG480700 Rh2DG517700 Rh2DG517800 Rh2DG517900 Rh5AG142400 Rh5BG140800 Rh5CG152900 Rh5DG141500
rosa_wichuraiana Rw2G040670 Rw2G040680 Rw2G040690 Rw5G012600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1025
AccB1I GGYRCC 1 cut(s) 1580
AccB7I CCANNNNNTGG 1 cut(s) 1214
AccBSI CCGCTC 1 cut(s) 1141
AccI GTMKAC 4 cut(s) 380, 454, 1498, 1548
AccII CGCG 1 cut(s) 254
AclWI GGATC 4 cut(s) 522, 905, 1382, 1399
AcoI YGGCCR 3 cut(s) 153, 439, 1335
AcsI RAATTY 3 cut(s) 467, 717, 1402
AcuI CTGAAG 3 cut(s) 948, 1338, 1526
AcyI GRCGYC 1 cut(s) 180
AdeI CACNNNGTG 1 cut(s) 433
AfaI GTAC 2 cut(s) 555, 1638
AfiI CCNNNNNNNGG 4 cut(s) 151, 946, 1214, 1576
AgsI TTSAA 4 cut(s) 467, 830, 1387, 1423
AjnI CCWGG 1 cut(s) 939
Alw21I GWGCWC 2 cut(s) 1051, 1626
Alw26I GTCTC 3 cut(s) 1258, 1421, 1568
AlwI GGATC 4 cut(s) 522, 905, 1382, 1399
Ama87I CYCGRG 2 cut(s) 972, 1571
AoxI GGCC 5 cut(s) 142, 153, 439, 937, 1335
ApeKI GCWGC 7 cut(s) 325, 420, 532, 744, 896, 899, 1609
ApoI RAATTY 3 cut(s) 467, 717, 1402
ArsI GACNNNNNNTTYG 2 cut(s) 177, 209
Asp700I GAANNNNTTC 1 cut(s) 354
AspLEI GCGC 2 cut(s) 886, 1728
AspS9I GGNCC 2 cut(s) 142, 769
AsuC2I CCSGG 5 cut(s) 965, 973, 974, 1372, 1681
AsuHPI GGTGA 5 cut(s) 31, 112, 181, 403, 1088
AsuNHI GCTAGC 1 cut(s) 1327
AvaI CYCGRG 2 cut(s) 972, 1571
AvaII GGWCC 1 cut(s) 769
AxyI CCTNAGG 1 cut(s) 915
BaeGI GKGCMC 1 cut(s) 1583
BalI TGGCCA 1 cut(s) 1337
BanI GGYRCC 1 cut(s) 1580
BanII GRGCYC 1 cut(s) 1626
BarI GAAGNNNNNNTAC 2 cut(s) 217, 249
BauI CACGAG 2 cut(s) 590, 1050
BbsI GAAGAC 1 cut(s) 1680
Bbv12I GWGCWC 2 cut(s) 1051, 1626
BbvCI CCTCAGC 1 cut(s) 128
BbvI GCAGC 7 cut(s) 312, 407, 544, 731, 883, 886, 1621
BccI CCATC 3 cut(s) 28, 1024, 1402
BceAI ACGGC 2 cut(s) 399, 749
BcgI CGANNNNNNTGC 2 cut(s) 863, 897
BciT130I CCWGG 1 cut(s) 941
BclI TGATCA 1 cut(s) 628
BcnI CCSGG 5 cut(s) 965, 973, 974, 1372, 1681
BcoDI GTCTC 3 cut(s) 1258, 1421, 1568
BfaI CTAG 5 cut(s) 677, 1184, 1328, 1395, 1461
BfmI CTRYAG 1 cut(s) 172
BglI GCCNNNNNGGC 1 cut(s) 334
BglII AGATCT 1 cut(s) 658
Bme1390I CCNGG 6 cut(s) 941, 965, 973, 974, 1372, 1681
Bme18I GGWCC 1 cut(s) 769
BmeT110I CYCGRG 2 cut(s) 972, 1571
BmgT120I GGNCC 2 cut(s) 142, 769
BmiI GGNNCC 2 cut(s) 771, 1582
BmrFI CCNGG 6 cut(s) 941, 965, 973, 974, 1372, 1681
BmsI GCATC 3 cut(s) 1064, 1185, 1232
BmtI GCTAGC 1 cut(s) 1331
BpiI GAAGAC 1 cut(s) 1680
BpmI CTGGAG 2 cut(s) 1236, 1298
Bpu10I CCTNAGC 1 cut(s) 128
BpuEI CTTGAG 3 cut(s) 52, 388, 1610
BpuMI CCSGG 5 cut(s) 965, 973, 974, 1372, 1681
Bsa29I ATCGAT 1 cut(s) 546
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 5 cut(s) 765, 940, 972, 973, 1332
BsaWI WCCGGW 3 cut(s) 205, 403, 1164
BsaXI ACNNNNNCTCC 6 cut(s) 31, 61, 116, 146, 470, 500
Bsc4I CCNNNNNNNGG 4 cut(s) 151, 946, 1214, 1576
Bse118I RCCGGY 1 cut(s) 144
Bse1I ACTGG 3 cut(s) 156, 816, 1281
Bse21I CCTNAGG 1 cut(s) 915
Bse3DI GCAATG 2 cut(s) 993, 1513
BseBI CCWGG 1 cut(s) 941
BseCI ATCGAT 1 cut(s) 546
BseDI CCNNGG 5 cut(s) 765, 940, 972, 973, 1332
BseGI GGATG 3 cut(s) 11, 577, 1694
BseLI CCNNNNNNNGG 4 cut(s) 151, 946, 1214, 1576
BseMI GCAATG 2 cut(s) 993, 1513
BseMII CTCAG 6 cut(s) 142, 669, 749, 831, 1128, 1353
BseNI ACTGG 3 cut(s) 156, 816, 1281
BseRI GAGGAG 4 cut(s) 34, 73, 253, 1690
BseSI GKGCMC 1 cut(s) 1583
BseXI GCAGC 7 cut(s) 312, 407, 544, 731, 883, 886, 1621
BsgI GTGCAG 2 cut(s) 1241, 1460
Bsh1236I CGCG 1 cut(s) 254
Bsh1285I CGRYCG 1 cut(s) 408
BshFI GGCC 5 cut(s) 144, 155, 441, 939, 1337
BshNI GGYRCC 1 cut(s) 1580
BshVI ATCGAT 1 cut(s) 546
BsiEI CGRYCG 1 cut(s) 408
BsiHKAI GWGCWC 2 cut(s) 1051, 1626
BsiHKCI CYCGRG 2 cut(s) 972, 1571
BslI CCNNNNNNNGG 4 cut(s) 151, 946, 1214, 1576
BsmAI GTCTC 3 cut(s) 1258, 1421, 1568
BsmBI CGTCTC 1 cut(s) 1568
BsmI GAATGC 1 cut(s) 513
BsnI GGCC 5 cut(s) 144, 155, 441, 939, 1337
BsoBI CYCGRG 2 cut(s) 972, 1571
Bsp1286I GDGCHC 3 cut(s) 1051, 1583, 1626
Bsp143I GATC 7 cut(s) 514, 628, 658, 910, 1252, 1374, 1391
Bsp19I CCATGG 1 cut(s) 1332
BspANI GGCC 5 cut(s) 144, 155, 441, 939, 1337
BspCNI CTCAG 6 cut(s) 141, 668, 748, 830, 1129, 1354
BspDI ATCGAT 1 cut(s) 546
BspFNI CGCG 1 cut(s) 254
BspLI GGNNCC 2 cut(s) 771, 1582
BspOI GCTAGC 1 cut(s) 1331
BspPI GGATC 4 cut(s) 522, 905, 1382, 1399
BspT107I GGYRCC 1 cut(s) 1580
BsrBI CCGCTC 1 cut(s) 1141
BsrDI GCAATG 2 cut(s) 993, 1513
BsrFI RCCGGY 1 cut(s) 144
BsrI ACTGG 3 cut(s) 156, 816, 1281
BssAI RCCGGY 1 cut(s) 144
BssECI CCNNGG 5 cut(s) 765, 940, 972, 973, 1332
BssMI GATC 7 cut(s) 514, 628, 658, 910, 1252, 1374, 1391
BssNAI GTATAC 1 cut(s) 381
BssNI GRCGYC 1 cut(s) 180
BssSI CACGAG 2 cut(s) 590, 1050
BssT1I CCWWGG 1 cut(s) 1332
Bst1107I GTATAC 1 cut(s) 381
Bst2BI CACGAG 2 cut(s) 590, 1050
Bst2UI CCWGG 1 cut(s) 941
Bst4CI ACNGT 4 cut(s) 193, 985, 1438, 1486
Bst6I CTCTTC 2 cut(s) 779, 1384
BstACI GRCGYC 1 cut(s) 180
BstC8I GCNNGC 3 cut(s) 146, 762, 1329
BstDEI CTNAG 8 cut(s) 128, 655, 735, 817, 868, 915, 1137, 1362
BstDSI CCRYGG 2 cut(s) 765, 1332
BstF5I GGATG 3 cut(s) 11, 577, 1694
BstFNI CGCG 1 cut(s) 254
BstHHI GCGC 2 cut(s) 886, 1728
BstKTI GATC 7 cut(s) 517, 631, 661, 913, 1255, 1377, 1394
BstMAI GTCTC 3 cut(s) 1258, 1421, 1568
BstMBI GATC 7 cut(s) 514, 628, 658, 910, 1252, 1374, 1391
BstMCI CGRYCG 1 cut(s) 408
BstMWI GCNNNNNNNGC 6 cut(s) 174, 334, 538, 1012, 1220, 1328
BstNI CCWGG 1 cut(s) 941
BstNSI RCATGY 2 cut(s) 341, 760
BstSCI CCNGG 6 cut(s) 939, 963, 971, 972, 1370, 1679
BstSFI CTRYAG 1 cut(s) 172
BstSLI GKGCMC 1 cut(s) 1583
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 7 cut(s) 312, 407, 544, 731, 883, 886, 1621
BstV2I GAAGAC 1 cut(s) 1680
BstX2I RGATCY 4 cut(s) 514, 658, 1374, 1391
BstYI RGATCY 4 cut(s) 514, 658, 1374, 1391
BstZ17I GTATAC 1 cut(s) 381
Bsu15I ATCGAT 1 cut(s) 546
Bsu36I CCTNAGG 1 cut(s) 915
BsuRI GGCC 5 cut(s) 144, 155, 441, 939, 1337
BsuTUI ATCGAT 1 cut(s) 546
BtgI CCRYGG 2 cut(s) 765, 1332
BtsCI GGATG 3 cut(s) 11, 577, 1694
BtsI GCAGTG 1 cut(s) 739
BtsIMutI CAGTG 6 cut(s) 638, 739, 809, 990, 1434, 1584
Cac8I GCNNGC 3 cut(s) 146, 762, 1329
CfoI GCGC 2 cut(s) 886, 1728
Cfr10I RCCGGY 1 cut(s) 144
Cfr13I GGNCC 2 cut(s) 142, 769
Cfr9I CCCGGG 1 cut(s) 972
ClaI ATCGAT 1 cut(s) 546
CseI GACGC 1 cut(s) 188
Csp6I GTAC 2 cut(s) 554, 1637
CspCI CAANNNNNGTGG 2 cut(s) 573, 608
CviAII CATG 5 cut(s) 338, 443, 757, 1261, 1333
CviQI GTAC 2 cut(s) 554, 1637
DdeI CTNAG 8 cut(s) 128, 655, 735, 817, 868, 915, 1137, 1362
DpnI GATC 7 cut(s) 516, 630, 660, 912, 1254, 1376, 1393
DpnII GATC 7 cut(s) 514, 628, 658, 910, 1252, 1374, 1391
DraIII CACNNNGTG 1 cut(s) 433
EaeI YGGCCR 3 cut(s) 153, 439, 1335
Eam1104I CTCTTC 2 cut(s) 779, 1384
EarI CTCTTC 2 cut(s) 779, 1384
EciI GGCGGA 2 cut(s) 76, 584
Ecl136II GAGCTC 1 cut(s) 1624
Eco130I CCWWGG 1 cut(s) 1332
Eco24I GRGCYC 1 cut(s) 1626
Eco47I GGWCC 1 cut(s) 769
Eco53kI GAGCTC 1 cut(s) 1624
Eco57I CTGAAG 3 cut(s) 948, 1338, 1526
Eco81I CCTNAGG 1 cut(s) 915
Eco88I CYCGRG 2 cut(s) 972, 1571
EcoICRI GAGCTC 1 cut(s) 1624
EcoRII CCWGG 1 cut(s) 939
EcoT14I CCWWGG 1 cut(s) 1332
EcoT38I GRGCYC 1 cut(s) 1626
ErhI CCWWGG 1 cut(s) 1332
Esp3I CGTCTC 1 cut(s) 1568
FaeI CATG 5 cut(s) 341, 446, 760, 1264, 1336
FatI CATG 5 cut(s) 337, 442, 756, 1260, 1332
FauI CCCGC 4 cut(s) 105, 261, 466, 1134
FbaI TGATCA 1 cut(s) 628
FblI GTMKAC 4 cut(s) 380, 454, 1498, 1548
FokI GGATG 2 cut(s) 584, 1681
FriOI GRGCYC 1 cut(s) 1626
FspBI CTAG 5 cut(s) 677, 1184, 1328, 1395, 1461
GlaI GCGC 2 cut(s) 885, 1727
GsuI CTGGAG 2 cut(s) 1236, 1298
HaeIII GGCC 5 cut(s) 144, 155, 441, 939, 1337
HgaI GACGC 1 cut(s) 188
HhaI GCGC 2 cut(s) 886, 1728
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 5 cut(s) 341, 446, 760, 1264, 1336
Hin6I GCGC 2 cut(s) 884, 1726
HinP1I GCGC 2 cut(s) 884, 1726
HincII GTYRAC 4 cut(s) 455, 958, 1107, 1549
HindII GTYRAC 4 cut(s) 455, 958, 1107, 1549
HindIII AAGCTT 1 cut(s) 447
HpaI GTTAAC 1 cut(s) 958
HphI GGTGA 5 cut(s) 31, 112, 181, 403, 1088
Hpy166II GTNNAC 9 cut(s) 217, 381, 427, 455, 769, 958, 1107, 1499, 1549
Hpy188III TCNNGA 8 cut(s) 367, 662, 677, 692, 793, 1184, 1556, 1573
Hpy8I GTNNAC 9 cut(s) 217, 381, 427, 455, 769, 958, 1107, 1499, 1549
Hpy99I CGWCG 2 cut(s) 182, 197
HpyAV CCTTC 4 cut(s) 287, 1067, 1550, 1562
HpyCH4III ACNGT 4 cut(s) 193, 985, 1438, 1486
HpyCH4V TGCA 6 cut(s) 532, 1055, 1173, 1198, 1258, 1441
HpyF10VI GCNNNNNNNGC 6 cut(s) 174, 334, 538, 1012, 1220, 1328
HpyF3I CTNAG 8 cut(s) 128, 655, 735, 817, 868, 915, 1137, 1362
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 5 cut(s) 341, 446, 760, 1264, 1336
HspAI GCGC 2 cut(s) 884, 1726
KroI GCCGGC 1 cut(s) 144
KroNI GCCGGC 1 cut(s) 146
Ksp22I TGATCA 1 cut(s) 628
KspAI GTTAAC 1 cut(s) 958
Kzo9I GATC 7 cut(s) 514, 628, 658, 910, 1252, 1374, 1391
LmnI GCTCC 3 cut(s) 137, 240, 1621
Lsp1109I GCAGC 7 cut(s) 312, 407, 544, 731, 883, 886, 1621
LweI GCATC 3 cut(s) 1064, 1185, 1232
MaeI CTAG 5 cut(s) 677, 1184, 1328, 1395, 1461
MaeIII GTNAC 2 cut(s) 344, 862
MalI GATC 7 cut(s) 516, 630, 660, 912, 1254, 1376, 1393
MbiI CCGCTC 1 cut(s) 1141
MboI GATC 7 cut(s) 514, 628, 658, 910, 1252, 1374, 1391
MboII GAAGA 9 cut(s) 15, 486, 713, 721, 796, 1224, 1371, 1643, 1680
MflI RGATCY 4 cut(s) 514, 658, 1374, 1391
MhlI GDGCHC 3 cut(s) 1051, 1583, 1626
MlsI TGGCCA 1 cut(s) 1337
MluCI AATT 7 cut(s) 7, 136, 467, 717, 730, 1020, 1402
MluNI TGGCCA 1 cut(s) 1337
MlyI GAGTC 5 cut(s) 152, 809, 863, 1423, 1446
MmeI TCCRAC 4 cut(s) 39, 84, 117, 1446
Mox20I TGGCCA 1 cut(s) 1337
MroNI GCCGGC 1 cut(s) 144
MroXI GAANNNNTTC 1 cut(s) 354
MscI TGGCCA 1 cut(s) 1337
MseI TTAA 4 cut(s) 614, 714, 848, 957
MslI CAYNNNNRTG 1 cut(s) 998
Msp20I TGGCCA 1 cut(s) 1337
MspR9I CCNGG 6 cut(s) 941, 965, 973, 974, 1372, 1681
Mva1269I GAATGC 1 cut(s) 513
MvaI CCWGG 1 cut(s) 941
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 6 cut(s) 174, 334, 538, 1012, 1220, 1328
NaeI GCCGGC 1 cut(s) 146
NciI CCSGG 5 cut(s) 965, 973, 974, 1372, 1681
NcoI CCATGG 1 cut(s) 1332
NdeII GATC 7 cut(s) 514, 628, 658, 910, 1252, 1374, 1391
NgoMIV GCCGGC 1 cut(s) 144
NheI GCTAGC 1 cut(s) 1327
NlaIII CATG 5 cut(s) 341, 446, 760, 1264, 1336
NlaIV GGNNCC 2 cut(s) 771, 1582
NspI RCATGY 2 cut(s) 341, 760
PaeR7I CTCGAG 1 cut(s) 1571
PctI GAATGC 1 cut(s) 513
PdiI GCCGGC 1 cut(s) 146
PdmI GAANNNNTTC 1 cut(s) 354
PfeI GAWTC 6 cut(s) 281, 789, 968, 1204, 1282, 1469
PflMI CCANNNNNTGG 1 cut(s) 1214
PfoI TCCNGGA 1 cut(s) 963
PleI GAGTC 5 cut(s) 152, 809, 862, 1423, 1446
PpsI GAGTC 5 cut(s) 152, 809, 862, 1423, 1446
PsiI TTATAA 1 cut(s) 1025
Psp124BI GAGCTC 1 cut(s) 1626
Psp6I CCWGG 1 cut(s) 939
PspGI CCWGG 1 cut(s) 939
PspN4I GGNNCC 2 cut(s) 771, 1582
PspPI GGNCC 2 cut(s) 142, 769
PsuI RGATCY 4 cut(s) 514, 658, 1374, 1391
RsaI GTAC 2 cut(s) 555, 1638
RsaNI GTAC 2 cut(s) 554, 1637
RseI CAYNNNNRTG 1 cut(s) 998
SacI GAGCTC 1 cut(s) 1626
SalI GTCGAC 2 cut(s) 453, 1547
SaqAI TTAA 4 cut(s) 614, 714, 848, 957
Sau3AI GATC 7 cut(s) 514, 628, 658, 910, 1252, 1374, 1391
Sau96I GGNCC 2 cut(s) 142, 769
SchI GAGTC 5 cut(s) 152, 809, 863, 1423, 1446
ScrFI CCNGG 6 cut(s) 941, 965, 973, 974, 1372, 1681
SduI GDGCHC 3 cut(s) 1051, 1583, 1626
SfaNI GCATC 3 cut(s) 1064, 1185, 1232
SfcI CTRYAG 1 cut(s) 172
Sfr274I CTCGAG 1 cut(s) 1571
SinI GGWCC 1 cut(s) 769
SlaI CTCGAG 1 cut(s) 1571
SmaI CCCGGG 1 cut(s) 974
SmiMI CAYNNNNRTG 1 cut(s) 998
SmlI CTYRAG 4 cut(s) 31, 367, 1571, 1625
SmoI CTYRAG 4 cut(s) 31, 367, 1571, 1625
Sse9I AATT 7 cut(s) 7, 136, 467, 717, 730, 1020, 1402
SspI AATATT 1 cut(s) 1029
SspMI CTAG 5 cut(s) 677, 1184, 1328, 1395, 1461
SstI GAGCTC 1 cut(s) 1626
StyD4I CCNGG 6 cut(s) 939, 963, 971, 972, 1370, 1679
StyI CCWWGG 1 cut(s) 1332
TaaI ACNGT 4 cut(s) 193, 985, 1438, 1486
TasI AATT 7 cut(s) 7, 136, 467, 717, 730, 1020, 1402
TatI WGTACW 2 cut(s) 553, 1636
TauI GCSGC 4 cut(s) 103, 168, 441, 729
TfiI GAWTC 6 cut(s) 281, 789, 968, 1204, 1282, 1469
Tru1I TTAA 4 cut(s) 614, 714, 848, 957
Tru9I TTAA 4 cut(s) 614, 714, 848, 957
TscAI CASTG 6 cut(s) 638, 746, 816, 990, 1441, 1591
TseI GCWGC 7 cut(s) 325, 420, 532, 744, 896, 899, 1609
TspDTI ATGAA 4 cut(s) 459, 593, 851, 878
TspMI CCCGGG 1 cut(s) 972
TspRI CASTG 6 cut(s) 638, 746, 816, 990, 1441, 1591
Van91I CCANNNNNTGG 1 cut(s) 1214
VpaK11BI GGWCC 1 cut(s) 769
XapI RAATTY 3 cut(s) 467, 717, 1402
XbaI TCTAGA 2 cut(s) 676, 1183
XceI RCATGY 2 cut(s) 341, 760
XcmI CCANNNNNNNNNTGG 1 cut(s) 1330
XhoI CTCGAG 1 cut(s) 1571
XmaI CCCGGG 1 cut(s) 972
XmiI GTMKAC 4 cut(s) 380, 454, 1498, 1548
XmnI GAANNNNTTC 1 cut(s) 354
XspI CTAG 5 cut(s) 677, 1184, 1328, 1395, 1461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.