Regulatory protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 784 |
| Acc36I | ACCTGC | 3 cut(s) | 440, 478, 784 |
| AccB1I | GGYRCC | 2 cut(s) | 106, 549 |
| AccBSI | CCGCTC | 1 cut(s) | 547 |
| AccI | GTMKAC | 2 cut(s) | 392, 466 |
| AccII | CGCG | 1 cut(s) | 267 |
| AccIII | TCCGGA | 1 cut(s) | 975 |
| AciI | CCGC | 3 cut(s) | 504, 547, 739 |
| AclWI | GGATC | 3 cut(s) | 164, 776, 917 |
| AcsI | RAATTY | 2 cut(s) | 729, 979 |
| AcyI | GRCGYC | 1 cut(s) | 550 |
| AfaI | GTAC | 3 cut(s) | 228, 320, 500 |
| AflII | CTTAAG | 2 cut(s) | 379, 404 |
| AgsI | TTSAA | 5 cut(s) | 69, 119, 277, 628, 794 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 289 |
| AjnI | CCWGG | 1 cut(s) | 172 |
| Alw26I | GTCTC | 1 cut(s) | 276 |
| AlwI | GGATC | 3 cut(s) | 164, 776, 917 |
| Aor13HI | TCCGGA | 1 cut(s) | 975 |
| AoxI | GGCC | 2 cut(s) | 311, 955 |
| ApeKI | GCWGC | 2 cut(s) | 908, 911 |
| ApoI | RAATTY | 2 cut(s) | 729, 979 |
| AspLEI | GCGC | 1 cut(s) | 552 |
| AspS9I | GGNCC | 1 cut(s) | 955 |
| AxyI | CCTNAGG | 1 cut(s) | 927 |
| BaeGI | GKGCMC | 1 cut(s) | 454 |
| BanI | GGYRCC | 2 cut(s) | 106, 549 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 229, 261 |
| BauI | CACGAG | 1 cut(s) | 602 |
| BbsI | GAAGAC | 1 cut(s) | 285 |
| BbvI | GCAGC | 2 cut(s) | 895, 923 |
| BccI | CCATC | 1 cut(s) | 28 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 929, 963 |
| BciT130I | CCWGG | 1 cut(s) | 174 |
| BcoDI | GTCTC | 1 cut(s) | 276 |
| BfaI | CTAG | 3 cut(s) | 147, 249, 984 |
| BfmI | CTRYAG | 1 cut(s) | 909 |
| BfoI | RGCGCY | 1 cut(s) | 553 |
| BfrI | CTTAAG | 2 cut(s) | 379, 404 |
| BfuAI | ACCTGC | 3 cut(s) | 440, 478, 784 |
| BisI | GCNGC | 5 cut(s) | 504, 548, 739, 909, 912 |
| BlpI | GCTNAGC | 1 cut(s) | 140 |
| BlsI | GCNGC | 5 cut(s) | 505, 549, 740, 910, 913 |
| BmcAI | AGTACT | 1 cut(s) | 320 |
| Bme1390I | CCNGG | 1 cut(s) | 174 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 289 |
| BmgT120I | GGNCC | 1 cut(s) | 955 |
| BmiI | GGNNCC | 3 cut(s) | 97, 108, 551 |
| BmrFI | CCNGG | 1 cut(s) | 174 |
| BmsI | GCATC | 1 cut(s) | 181 |
| BpiI | GAAGAC | 1 cut(s) | 285 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 140 |
| BpuEI | CTTGAG | 1 cut(s) | 710 |
| Bsa29I | ATCGAT | 1 cut(s) | 639 |
| BsaHI | GRCGYC | 1 cut(s) | 550 |
| BsaJI | CCNNGG | 1 cut(s) | 417 |
| BsaWI | WCCGGW | 1 cut(s) | 975 |
| Bse1I | ACTGG | 1 cut(s) | 400 |
| Bse21I | CCTNAGG | 1 cut(s) | 927 |
| Bse3DI | GCAATG | 1 cut(s) | 1004 |
| BseAI | TCCGGA | 1 cut(s) | 975 |
| BseBI | CCWGG | 1 cut(s) | 174 |
| BseCI | ATCGAT | 1 cut(s) | 639 |
| BseDI | CCNNGG | 1 cut(s) | 417 |
| BseGI | GGATG | 2 cut(s) | 23, 838 |
| BseMI | GCAATG | 1 cut(s) | 1004 |
| BseMII | CTCAG | 1 cut(s) | 23 |
| BseNI | ACTGG | 1 cut(s) | 400 |
| BseSI | GKGCMC | 1 cut(s) | 454 |
| BseXI | GCAGC | 2 cut(s) | 895, 923 |
| Bsh1236I | CGCG | 1 cut(s) | 267 |
| BshFI | GGCC | 2 cut(s) | 313, 957 |
| BshNI | GGYRCC | 2 cut(s) | 106, 549 |
| BshVI | ATCGAT | 1 cut(s) | 639 |
| BsiSI | CCGG | 1 cut(s) | 976 |
| BslFI | GGGAC | 1 cut(s) | 711 |
| BsmAI | GTCTC | 1 cut(s) | 276 |
| BsmFI | GGGAC | 1 cut(s) | 711 |
| BsmI | GAATGC | 1 cut(s) | 807 |
| BsnI | GGCC | 2 cut(s) | 313, 957 |
| Bsp1286I | GDGCHC | 1 cut(s) | 454 |
| Bsp13I | TCCGGA | 1 cut(s) | 975 |
| Bsp1407I | TGTACA | 1 cut(s) | 226 |
| Bsp143I | GATC | 5 cut(s) | 169, 640, 665, 781, 922 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 140 |
| Bsp68I | TCGCGA | 1 cut(s) | 267 |
| BspACI | CCGC | 3 cut(s) | 504, 547, 739 |
| BspANI | GGCC | 2 cut(s) | 313, 957 |
| BspCNI | CTCAG | 1 cut(s) | 24 |
| BspDI | ATCGAT | 1 cut(s) | 639 |
| BspEI | TCCGGA | 1 cut(s) | 975 |
| BspFNI | CGCG | 1 cut(s) | 267 |
| BspLI | GGNNCC | 3 cut(s) | 97, 108, 551 |
| BspMAI | CTGCAG | 1 cut(s) | 913 |
| BspMI | ACCTGC | 3 cut(s) | 440, 478, 784 |
| BspPI | GGATC | 3 cut(s) | 164, 776, 917 |
| BspT107I | GGYRCC | 2 cut(s) | 106, 549 |
| BspTI | CTTAAG | 2 cut(s) | 379, 404 |
| BsrBI | CCGCTC | 1 cut(s) | 547 |
| BsrDI | GCAATG | 1 cut(s) | 1004 |
| BsrGI | TGTACA | 1 cut(s) | 226 |
| BsrI | ACTGG | 1 cut(s) | 400 |
| BssECI | CCNNGG | 1 cut(s) | 417 |
| BssMI | GATC | 5 cut(s) | 169, 640, 665, 781, 922 |
| BssNAI | GTATAC | 1 cut(s) | 393 |
| BssNI | GRCGYC | 1 cut(s) | 550 |
| BssSI | CACGAG | 1 cut(s) | 602 |
| Bst1107I | GTATAC | 1 cut(s) | 393 |
| Bst2BI | CACGAG | 1 cut(s) | 602 |
| Bst2UI | CCWGG | 1 cut(s) | 174 |
| Bst4CI | ACNGT | 1 cut(s) | 323 |
| BstACI | GRCGYC | 1 cut(s) | 550 |
| BstAFI | CTTAAG | 2 cut(s) | 379, 404 |
| BstAUI | TGTACA | 1 cut(s) | 226 |
| BstC8I | GCNNGC | 2 cut(s) | 269, 948 |
| BstDEI | CTNAG | 4 cut(s) | 32, 140, 880, 927 |
| BstDSI | CCRYGG | 1 cut(s) | 417 |
| BstF5I | GGATG | 2 cut(s) | 23, 838 |
| BstFNI | CGCG | 1 cut(s) | 267 |
| BstH2I | RGCGCY | 1 cut(s) | 553 |
| BstHHI | GCGC | 1 cut(s) | 552 |
| BstKTI | GATC | 5 cut(s) | 172, 643, 668, 784, 925 |
| BstMAI | GTCTC | 1 cut(s) | 276 |
| BstMBI | GATC | 5 cut(s) | 169, 640, 665, 781, 922 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 11, 277, 458 |
| BstNI | CCWGG | 1 cut(s) | 174 |
| BstNSI | RCATGY | 2 cut(s) | 465, 999 |
| BstSCI | CCNGG | 1 cut(s) | 172 |
| BstSFI | CTRYAG | 1 cut(s) | 909 |
| BstSLI | GKGCMC | 1 cut(s) | 454 |
| BstUI | CGCG | 1 cut(s) | 267 |
| BstV1I | GCAGC | 2 cut(s) | 895, 923 |
| BstV2I | GAAGAC | 1 cut(s) | 285 |
| BstZ17I | GTATAC | 1 cut(s) | 393 |
| Bsu15I | ATCGAT | 1 cut(s) | 639 |
| Bsu36I | CCTNAGG | 1 cut(s) | 927 |
| BsuRI | GGCC | 2 cut(s) | 313, 957 |
| BsuTUI | ATCGAT | 1 cut(s) | 639 |
| BtgI | CCRYGG | 1 cut(s) | 417 |
| BtgZI | GCGATG | 2 cut(s) | 30, 363 |
| BtsCI | GGATG | 2 cut(s) | 23, 838 |
| BtsIMutI | CAGTG | 1 cut(s) | 650 |
| BtuMI | TCGCGA | 1 cut(s) | 267 |
| BveI | ACCTGC | 3 cut(s) | 440, 478, 784 |
| Cac8I | GCNNGC | 2 cut(s) | 269, 948 |
| CfoI | GCGC | 1 cut(s) | 552 |
| Cfr13I | GGNCC | 1 cut(s) | 955 |
| ClaI | ATCGAT | 1 cut(s) | 639 |
| CseI | GACGC | 1 cut(s) | 467 |
| Csp6I | GTAC | 3 cut(s) | 227, 319, 499 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 585, 620 |
| CviAII | CATG | 3 cut(s) | 455, 462, 996 |
| CviQI | GTAC | 3 cut(s) | 227, 319, 499 |
| DdeI | CTNAG | 4 cut(s) | 32, 140, 880, 927 |
| DinI | GGCGCC | 1 cut(s) | 551 |
| DpnI | GATC | 5 cut(s) | 171, 642, 667, 783, 924 |
| DpnII | GATC | 5 cut(s) | 169, 640, 665, 781, 922 |
| DriI | GACNNNNNGTC | 1 cut(s) | 289 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 289 |
| Eco32I | GATATC | 1 cut(s) | 199 |
| Eco81I | CCTNAGG | 1 cut(s) | 927 |
| EcoRI | GAATTC | 1 cut(s) | 979 |
| EcoRII | CCWGG | 1 cut(s) | 172 |
| EcoRV | GATATC | 1 cut(s) | 199 |
| EgeI | GGCGCC | 1 cut(s) | 551 |
| EheI | GGCGCC | 1 cut(s) | 551 |
| FaeI | CATG | 3 cut(s) | 458, 465, 999 |
| FaqI | GGGAC | 1 cut(s) | 711 |
| FatI | CATG | 3 cut(s) | 454, 461, 995 |
| FblI | GTMKAC | 2 cut(s) | 392, 466 |
| Fnu4HI | GCNGC | 5 cut(s) | 504, 548, 739, 909, 912 |
| FokI | GGATG | 2 cut(s) | 10, 845 |
| Fsp4HI | GCNGC | 5 cut(s) | 504, 548, 739, 909, 912 |
| FspBI | CTAG | 3 cut(s) | 147, 249, 984 |
| GlaI | GCGC | 1 cut(s) | 551 |
| GluI | GCNGC | 5 cut(s) | 504, 548, 739, 909, 912 |
| HaeII | RGCGCY | 1 cut(s) | 553 |
| HaeIII | GGCC | 2 cut(s) | 313, 957 |
| HapII | CCGG | 1 cut(s) | 976 |
| HgaI | GACGC | 1 cut(s) | 467 |
| HhaI | GCGC | 1 cut(s) | 552 |
| Hin1I | GRCGYC | 1 cut(s) | 550 |
| Hin1II | CATG | 3 cut(s) | 458, 465, 999 |
| Hin6I | GCGC | 1 cut(s) | 550 |
| HinP1I | GCGC | 1 cut(s) | 550 |
| HincII | GTYRAC | 2 cut(s) | 429, 970 |
| HindII | GTYRAC | 2 cut(s) | 429, 970 |
| HindIII | AAGCTT | 2 cut(s) | 137, 401 |
| HinfI | GANTC | 6 cut(s) | 101, 214, 628, 755, 827, 866 |
| HpaI | GTTAAC | 1 cut(s) | 970 |
| HpaII | CCGG | 1 cut(s) | 976 |
| Hpy166II | GTNNAC | 7 cut(s) | 227, 229, 393, 429, 439, 467, 970 |
| Hpy188I | TCNGA | 6 cut(s) | 33, 100, 583, 670, 760, 832 |
| Hpy188III | TCNNGA | 6 cut(s) | 116, 266, 297, 689, 704, 976 |
| Hpy8I | GTNNAC | 7 cut(s) | 227, 229, 393, 429, 439, 467, 970 |
| HpyAV | CCTTC | 4 cut(s) | 376, 420, 563, 688 |
| HpyCH4III | ACNGT | 1 cut(s) | 323 |
| HpyCH4V | TGCA | 1 cut(s) | 911 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 11, 277, 458 |
| HpyF3I | CTNAG | 4 cut(s) | 32, 140, 880, 927 |
| Hsp92I | GRCGYC | 1 cut(s) | 550 |
| Hsp92II | CATG | 3 cut(s) | 458, 465, 999 |
| HspAI | GCGC | 1 cut(s) | 550 |
| KasI | GGCGCC | 1 cut(s) | 549 |
| Kpn2I | TCCGGA | 1 cut(s) | 975 |
| KspAI | GTTAAC | 1 cut(s) | 970 |
| Kzo9I | GATC | 5 cut(s) | 169, 640, 665, 781, 922 |
| LmnI | GCTCC | 1 cut(s) | 101 |
| Lsp1109I | GCAGC | 2 cut(s) | 895, 923 |
| LweI | GCATC | 1 cut(s) | 181 |
| MaeI | CTAG | 3 cut(s) | 147, 249, 984 |
| MaeIII | GTNAC | 1 cut(s) | 934 |
| MalI | GATC | 5 cut(s) | 171, 642, 667, 783, 924 |
| MbiI | CCGCTC | 1 cut(s) | 547 |
| MboI | GATC | 5 cut(s) | 169, 640, 665, 781, 922 |
| MboII | GAAGA | 4 cut(s) | 285, 725, 730, 819 |
| MhlI | GDGCHC | 1 cut(s) | 454 |
| MluCI | AATT | 7 cut(s) | 478, 491, 559, 597, 729, 742, 979 |
| Mly113I | GGCGCC | 1 cut(s) | 550 |
| MlyI | GAGTC | 2 cut(s) | 821, 875 |
| MmeI | TCCRAC | 1 cut(s) | 334 |
| MnlI | CCTC | 7 cut(s) | 37, 202, 298, 415, 703, 968, 983 |
| MroI | TCCGGA | 1 cut(s) | 975 |
| MseI | TTAA | 5 cut(s) | 380, 405, 558, 860, 969 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1009 |
| MspA1I | CMGCKG | 1 cut(s) | 14 |
| MspCI | CTTAAG | 2 cut(s) | 379, 404 |
| MspI | CCGG | 1 cut(s) | 976 |
| MspR9I | CCNGG | 1 cut(s) | 174 |
| Mva1269I | GAATGC | 1 cut(s) | 807 |
| MvaI | CCWGG | 1 cut(s) | 174 |
| MvnI | CGCG | 1 cut(s) | 267 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 11, 277, 458 |
| NarI | GGCGCC | 1 cut(s) | 550 |
| NdeII | GATC | 5 cut(s) | 169, 640, 665, 781, 922 |
| NlaIII | CATG | 3 cut(s) | 458, 465, 999 |
| NlaIV | GGNNCC | 3 cut(s) | 97, 108, 551 |
| NmuCI | GTSAC | 1 cut(s) | 934 |
| NruI | TCGCGA | 1 cut(s) | 267 |
| NspI | RCATGY | 2 cut(s) | 465, 999 |
| PaqCI | CACCTGC | 1 cut(s) | 784 |
| PctI | GAATGC | 1 cut(s) | 807 |
| PfeI | GAWTC | 4 cut(s) | 101, 214, 628, 755 |
| PkrI | GCNGC | 5 cut(s) | 505, 549, 740, 910, 913 |
| PleI | GAGTC | 2 cut(s) | 821, 874 |
| PluTI | GGCGCC | 1 cut(s) | 553 |
| PpsI | GAGTC | 2 cut(s) | 821, 874 |
| Psp6I | CCWGG | 1 cut(s) | 172 |
| PspGI | CCWGG | 1 cut(s) | 172 |
| PspN4I | GGNNCC | 3 cut(s) | 97, 108, 551 |
| PspPI | GGNCC | 1 cut(s) | 955 |
| PstI | CTGCAG | 1 cut(s) | 913 |
| PvuII | CAGCTG | 1 cut(s) | 14 |
| RruI | TCGCGA | 1 cut(s) | 267 |
| RsaI | GTAC | 3 cut(s) | 228, 320, 500 |
| RsaNI | GTAC | 3 cut(s) | 227, 319, 499 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1009 |
| SaqAI | TTAA | 5 cut(s) | 380, 405, 558, 860, 969 |
| SatI | GCNGC | 5 cut(s) | 504, 548, 739, 909, 912 |
| Sau3AI | GATC | 5 cut(s) | 169, 640, 665, 781, 922 |
| Sau96I | GGNCC | 1 cut(s) | 955 |
| ScaI | AGTACT | 1 cut(s) | 320 |
| SchI | GAGTC | 2 cut(s) | 821, 875 |
| ScrFI | CCNGG | 1 cut(s) | 174 |
| SduI | GDGCHC | 1 cut(s) | 454 |
| SfaNI | GCATC | 1 cut(s) | 181 |
| SfcI | CTRYAG | 1 cut(s) | 909 |
| SfoI | GGCGCC | 1 cut(s) | 551 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1009 |
| SmlI | CTYRAG | 3 cut(s) | 379, 404, 689 |
| SmoI | CTYRAG | 3 cut(s) | 379, 404, 689 |
| Sse9I | AATT | 7 cut(s) | 478, 491, 559, 597, 729, 742, 979 |
| SsiI | CCGC | 3 cut(s) | 504, 547, 739 |
| SspDI | GGCGCC | 1 cut(s) | 549 |
| SspMI | CTAG | 3 cut(s) | 147, 249, 984 |
| StyD4I | CCNGG | 1 cut(s) | 172 |
| TaaI | ACNGT | 1 cut(s) | 323 |
| TaqI | TCGA | 4 cut(s) | 536, 543, 639, 840 |
| TaqII | GACCGA | 1 cut(s) | 953 |
| TasI | AATT | 7 cut(s) | 478, 491, 559, 597, 729, 742, 979 |
| TatI | WGTACW | 2 cut(s) | 226, 318 |
| TauI | GCSGC | 3 cut(s) | 506, 550, 741 |
| TfiI | GAWTC | 4 cut(s) | 101, 214, 628, 755 |
| Tru1I | TTAA | 5 cut(s) | 380, 405, 558, 860, 969 |
| Tru9I | TTAA | 5 cut(s) | 380, 405, 558, 860, 969 |
| TscAI | CASTG | 1 cut(s) | 650 |
| TseFI | GTSAC | 1 cut(s) | 934 |
| TseI | GCWGC | 2 cut(s) | 908, 911 |
| Tsp45I | GTSAC | 1 cut(s) | 934 |
| TspDTI | ATGAA | 3 cut(s) | 33, 375, 900 |
| TspGWI | ACGGA | 1 cut(s) | 878 |
| TspRI | CASTG | 1 cut(s) | 650 |
| Vha464I | CTTAAG | 2 cut(s) | 379, 404 |
| XapI | RAATTY | 2 cut(s) | 729, 979 |
| XceI | RCATGY | 2 cut(s) | 465, 999 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 59 |
| XmiI | GTMKAC | 2 cut(s) | 392, 466 |
| XspI | CTAG | 3 cut(s) | 147, 249, 984 |
| ZrmI | AGTACT | 1 cut(s) | 320 |