MD06G1153400.v1.1

Catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001670
Physical Location & Seq
Forward (+)
29650398 .. 29659317
8920 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1153400.v1.1.491

Sequence Viewer

Length: 867 bp
ATGGCGACTGCAATGCTGCCAATATCCGAGCCCGCCAGAAAGCAACCCCGACCGGGTCGGGGCGGATTCAATGCCCATGGACCATCCGAAAAAGAGGCCCGGGTTCAAGCCATCGCCGAAATCGTCAGCTCCATGGTCGACCTCTCCCGGAAGGGCCAAAACGTCGACCTAAACGGCCTCAAGACCACCGCGTGCCGCAAGTACGGCCGTTCACGTGCCCCCACATCGCCATCACCGGGAATATTTGCGTGTACTGCCCCGGTGGTCCCGATTCGGACTCTGAGTACAGCACTCAGTCTTACACTGGATACGAGCCTACTAGCATGCGCGCAATTTGGGAAAGATTATTTGAAGGAATTGGTATACAACCCATATGTCCAGGCTAGAAGCAGGATAGATCAGCTGAAGACATTGGATCACAGTGTAGACAAGGTTTGGCCTGTTCTATGTTTAGAGCTTAAAGAATTTATTTTCGGGTTTGCAGATTTCCATGATAGAGTTGAGTTCATCTTAATGGGCGGTACTTTCATGTCGTTGCCCGCGGATTACCATGATTACTTTATTAGAAATCTGCATGATGCGTTATCGGGACACACTTCTGCCAATGTTGAAGAGGCAGTTGCCTACTCTGAGCATGGTGCTACTAAATGTATTGGAATGACCATTGAAACGCCAGATTATTGCCTTGGACCTCACTTGAGACAAATGCTTTCTTATGGTTGTACAAGACTAGAAATTGGAGTTCAGAGTACATACGAGGATGTGGCTCGGGACGCCAATAGAGGTCATACCGTAGCTGCAGTGGCTGATTGTTTTTGCTTGGCAAAGGATGCTGGTTTTAAGAGAACGCGGTGGTTGCATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0002097 GO:0002098 GO:0003674 GO:0003824 GO:0004402 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005719 GO:0005737 GO:0005829 GO:0006139 GO:0006325 GO:0006355 GO:0006357 GO:0006396 GO:0006399 GO:0006400 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0007389 GO:0008033 GO:0008080 GO:0008150 GO:0008152 GO:0008283 GO:0009292 GO:0009294 GO:0009451 GO:0009653 GO:0009798 GO:0009889 GO:0009966 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010646 GO:0010928 GO:0016043 GO:0016070 GO:0016407 GO:0016410 GO:0016569 GO:0016570 GO:0016573 GO:0016740 GO:0016746 GO:0016747 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0022603 GO:0023051 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033588 GO:0034212 GO:0034470 GO:0034641 GO:0034660 GO:0035265 GO:0036211 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0044764 GO:0046483 GO:0048583 GO:0048589 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051239 GO:0051252 GO:0051276 GO:0051704 GO:0060255 GO:0061733 GO:0065001 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090708 GO:0099402 GO:1900618 GO:1901360 GO:1901371 GO:1901564 GO:1903506 GO:1905392 GO:1905421 GO:1905428 GO:2000024 GO:2000025 GO:2000026 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Radical_SAM Radical_SAM_C

Protein Analysis

289

Amino Acids

31.73

Weight (kDa)

7.61

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ELP3_N PF23613 35 - 75 3e-08 ELP3 N-terminal domain
Radical_SAM PF04055 166 - 282 1.4e-08 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 4 cut(s) 138, 165, 363, 426
AccII CGCG 4 cut(s) 191, 329, 542, 850
AciI CCGC 8 cut(s) 33, 63, 189, 196, 519, 540, 542, 850
AclWI GGATC 1 cut(s) 423
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 1 cut(s) 464
AcuI CTGAAG 1 cut(s) 425
AcvI CACGTG 1 cut(s) 215
AcyI GRCGYC 1 cut(s) 774
AdeI CACNNNGTG 1 cut(s) 192
AfaI GTAC 6 cut(s) 203, 253, 286, 523, 724, 751
AfiI CCNNNNNNNGG 3 cut(s) 53, 59, 236
AgsI TTSAA 5 cut(s) 70, 107, 352, 611, 668
AjnI CCWGG 1 cut(s) 378
AluBI AGCT 4 cut(s) 129, 403, 457, 797
AluI AGCT 4 cut(s) 129, 403, 457, 797
Alw26I GTCTC 1 cut(s) 694
AlwI GGATC 1 cut(s) 423
AlwNI CAGNNNCTG 1 cut(s) 806
Ama87I CYCGRG 2 cut(s) 99, 768
AoxI GGCC 5 cut(s) 96, 154, 175, 205, 437
ApeKI GCWGC 2 cut(s) 16, 797
ApoI RAATTY 1 cut(s) 464
AspLEI GCGC 2 cut(s) 329, 331
AspS9I GGNCC 5 cut(s) 80, 97, 154, 265, 689
AsuC2I CCSGG 6 cut(s) 54, 100, 101, 148, 237, 260
AsuHPI GGTGA 1 cut(s) 225
AvaI CYCGRG 2 cut(s) 99, 768
AvaII GGWCC 3 cut(s) 80, 265, 689
BaeGI GKGCMC 1 cut(s) 220
BanII GRGCYC 1 cut(s) 33
BbrPI CACGTG 1 cut(s) 215
BbsI GAAGAC 1 cut(s) 413
BbvI GCAGC 2 cut(s) 3, 784
BccI CCATC 3 cut(s) 91, 119, 238
BceAI ACGGC 3 cut(s) 190, 192, 220
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 380
BciVI GTATCC 1 cut(s) 301
BcnI CCSGG 6 cut(s) 54, 100, 101, 148, 237, 260
BcoDI GTCTC 1 cut(s) 694
BfaI CTAG 3 cut(s) 320, 384, 731
BfmI CTRYAG 1 cut(s) 798
BfuI GTATCC 1 cut(s) 301
BisI GCNGC 3 cut(s) 17, 196, 798
BlsI GCNGC 3 cut(s) 18, 197, 799
Bme1390I CCNGG 7 cut(s) 54, 100, 101, 148, 237, 260, 380
Bme18I GGWCC 3 cut(s) 80, 265, 689
BmeT110I CYCGRG 2 cut(s) 99, 768
BmgT120I GGNCC 5 cut(s) 80, 97, 154, 265, 689
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 7 cut(s) 54, 100, 101, 148, 237, 260, 380
BmsI GCATC 2 cut(s) 568, 820
BpiI GAAGAC 1 cut(s) 413
BpuEI CTTGAG 2 cut(s) 164, 718
BpuMI CCSGG 6 cut(s) 54, 100, 101, 148, 237, 260
BsaAI YACGTR 1 cut(s) 215
BsaHI GRCGYC 1 cut(s) 774
BsaJI CCNNGG 6 cut(s) 76, 99, 132, 258, 540, 685
Bsc4I CCNNNNNNNGG 3 cut(s) 53, 59, 236
Bse1I ACTGG 1 cut(s) 309
Bse3DI GCAATG 1 cut(s) 18
BseBI CCWGG 1 cut(s) 380
BseDI CCNNGG 6 cut(s) 76, 99, 132, 258, 540, 685
BseGI GGATG 3 cut(s) 83, 766, 835
BseLI CCNNNNNNNGG 3 cut(s) 53, 59, 236
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 3 cut(s) 272, 307, 621
BseNI ACTGG 1 cut(s) 309
BsePI GCGCGC 1 cut(s) 327
BseSI GKGCMC 1 cut(s) 220
BseX3I CGGCCG 1 cut(s) 205
BseXI GCAGC 2 cut(s) 3, 784
Bsh1236I CGCG 4 cut(s) 191, 329, 542, 850
Bsh1285I CGRYCG 2 cut(s) 53, 208
BshFI GGCC 5 cut(s) 98, 156, 177, 207, 439
BsiEI CGRYCG 2 cut(s) 53, 208
BsiHKCI CYCGRG 2 cut(s) 99, 768
BsiSI CCGG 5 cut(s) 53, 100, 148, 236, 260
BslFI GGGAC 3 cut(s) 251, 603, 785
BslI CCNNNNNNNGG 3 cut(s) 53, 59, 236
BsmAI GTCTC 1 cut(s) 694
BsmFI GGGAC 3 cut(s) 251, 603, 785
BsnI GGCC 5 cut(s) 98, 156, 177, 207, 439
BsoBI CYCGRG 2 cut(s) 99, 768
Bsp1286I GDGCHC 2 cut(s) 33, 220
Bsp1407I TGTACA 1 cut(s) 722
Bsp143I GATC 2 cut(s) 397, 415
Bsp19I CCATGG 2 cut(s) 76, 132
BspACI CCGC 8 cut(s) 33, 63, 189, 196, 519, 540, 542, 850
BspANI GGCC 5 cut(s) 98, 156, 177, 207, 439
BspCNI CTCAG 3 cut(s) 273, 306, 622
BspFNI CGCG 4 cut(s) 191, 329, 542, 850
BspLI GGNNCC 1 cut(s) 267
BspMAI CTGCAG 1 cut(s) 802
BspPI GGATC 1 cut(s) 423
BsrDI GCAATG 1 cut(s) 18
BsrGI TGTACA 1 cut(s) 722
BsrI ACTGG 1 cut(s) 309
BssECI CCNNGG 6 cut(s) 76, 99, 132, 258, 540, 685
BssHII GCGCGC 1 cut(s) 327
BssMI GATC 2 cut(s) 397, 415
BssNAI GTATAC 1 cut(s) 364
BssNI GRCGYC 1 cut(s) 774
BssT1I CCWWGG 3 cut(s) 76, 132, 685
Bst1107I GTATAC 1 cut(s) 364
Bst2UI CCWGG 1 cut(s) 380
Bst4CI ACNGT 2 cut(s) 422, 793
Bst6I CTCTTC 1 cut(s) 606
BstACI GRCGYC 1 cut(s) 774
BstAPI GCANNNNNTGC 1 cut(s) 830
BstAUI TGTACA 1 cut(s) 722
BstBAI YACGTR 1 cut(s) 215
BstC8I GCNNGC 6 cut(s) 33, 193, 325, 329, 540, 861
BstDEI CTNAG 3 cut(s) 281, 293, 630
BstDSI CCRYGG 3 cut(s) 76, 132, 540
BstF5I GGATG 3 cut(s) 83, 766, 835
BstFNI CGCG 4 cut(s) 191, 329, 542, 850
BstHHI GCGC 2 cut(s) 329, 331
BstKTI GATC 2 cut(s) 400, 418
BstMAI GTCTC 1 cut(s) 694
BstMBI GATC 2 cut(s) 397, 415
BstMCI CGRYCG 2 cut(s) 53, 208
BstMWI GCNNNNNNNGC 6 cut(s) 204, 254, 773, 803, 830, 856
BstNI CCWGG 1 cut(s) 380
BstNSI RCATGY 2 cut(s) 327, 863
BstSCI CCNGG 7 cut(s) 52, 98, 99, 146, 235, 258, 378
BstSFI CTRYAG 1 cut(s) 798
BstSLI GKGCMC 1 cut(s) 220
BstUI CGCG 4 cut(s) 191, 329, 542, 850
BstV1I GCAGC 2 cut(s) 3, 784
BstV2I GAAGAC 1 cut(s) 413
BstZ17I GTATAC 1 cut(s) 364
BstZI CGGCCG 1 cut(s) 205
BsuI GTATCC 1 cut(s) 301
BsuRI GGCC 5 cut(s) 98, 156, 177, 207, 439
BtgI CCRYGG 3 cut(s) 76, 132, 540
BtgZI GCGATG 2 cut(s) 97, 210
BtsCI GGATG 3 cut(s) 83, 766, 835
BtsI GCAGTG 1 cut(s) 807
BtsIMutI CAGTG 3 cut(s) 302, 427, 807
Cac8I GCNNGC 6 cut(s) 33, 193, 325, 329, 540, 861
CaiI CAGNNNCTG 1 cut(s) 806
CfoI GCGC 2 cut(s) 329, 331
Cfr13I GGNCC 5 cut(s) 80, 97, 154, 265, 689
Cfr42I CCGCGG 1 cut(s) 543
Cfr9I CCCGGG 1 cut(s) 99
CseI GACGC 1 cut(s) 782
Csp6I GTAC 6 cut(s) 202, 252, 285, 522, 723, 750
CviQI GTAC 6 cut(s) 202, 252, 285, 522, 723, 750
DdeI CTNAG 3 cut(s) 281, 293, 630
DpnI GATC 2 cut(s) 399, 417
DpnII GATC 2 cut(s) 397, 415
DraIII CACNNNGTG 1 cut(s) 192
EaeI YGGCCR 1 cut(s) 205
EagI CGGCCG 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
EciI GGCGGA 1 cut(s) 78
EclXI CGGCCG 1 cut(s) 205
Eco130I CCWWGG 3 cut(s) 76, 132, 685
Eco24I GRGCYC 1 cut(s) 33
Eco47I GGWCC 3 cut(s) 80, 265, 689
Eco52I CGGCCG 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 425
Eco72I CACGTG 1 cut(s) 215
Eco88I CYCGRG 2 cut(s) 99, 768
EcoRII CCWGG 1 cut(s) 378
EcoT14I CCWWGG 3 cut(s) 76, 132, 685
EcoT22I ATGCAT 1 cut(s) 865
EcoT38I GRGCYC 1 cut(s) 33
ErhI CCWWGG 3 cut(s) 76, 132, 685
FaqI GGGAC 3 cut(s) 251, 603, 785
FauI CCCGC 2 cut(s) 40, 547
FauNDI CATATG 1 cut(s) 373
FblI GTMKAC 4 cut(s) 138, 165, 363, 426
Fnu4HI GCNGC 3 cut(s) 17, 196, 798
FokI GGATG 3 cut(s) 70, 773, 842
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 3 cut(s) 17, 196, 798
FspBI CTAG 3 cut(s) 320, 384, 731
GlaI GCGC 2 cut(s) 328, 330
GluI GCNGC 3 cut(s) 17, 196, 798
HaeIII GGCC 5 cut(s) 98, 156, 177, 207, 439
HapII CCGG 5 cut(s) 53, 100, 148, 236, 260
HgaI GACGC 1 cut(s) 782
HhaI GCGC 2 cut(s) 329, 331
Hin1I GRCGYC 1 cut(s) 774
Hin6I GCGC 2 cut(s) 327, 329
HinP1I GCGC 2 cut(s) 327, 329
HincII GTYRAC 2 cut(s) 139, 166
HindII GTYRAC 2 cut(s) 139, 166
HinfI GANTC 3 cut(s) 66, 271, 277
HpaII CCGG 5 cut(s) 53, 100, 148, 236, 260
HphI GGTGA 1 cut(s) 225
Hpy166II GTNNAC 6 cut(s) 139, 166, 212, 252, 364, 427
Hpy188I TCNGA 6 cut(s) 28, 88, 276, 282, 631, 747
Hpy188III TCNNGA 4 cut(s) 181, 268, 588, 770
Hpy8I GTNNAC 6 cut(s) 139, 166, 212, 252, 364, 427
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 2 cut(s) 145, 346
HpyCH4III ACNGT 2 cut(s) 422, 793
HpyCH4IV ACGT 2 cut(s) 162, 214
HpyCH4V TGCA 6 cut(s) 11, 482, 574, 800, 859, 863
HpyF10VI GCNNNNNNNGC 6 cut(s) 204, 254, 773, 803, 830, 856
HpyF3I CTNAG 3 cut(s) 281, 293, 630
HpySE526I ACGT 2 cut(s) 162, 214
Hsp92I GRCGYC 1 cut(s) 774
HspAI GCGC 2 cut(s) 327, 329
KspI CCGCGG 1 cut(s) 543
Kzo9I GATC 2 cut(s) 397, 415
LmnI GCTCC 1 cut(s) 134
Lsp1109I GCAGC 2 cut(s) 3, 784
LweI GCATC 2 cut(s) 568, 820
MaeI CTAG 3 cut(s) 320, 384, 731
MaeII ACGT 2 cut(s) 162, 214
MalI GATC 2 cut(s) 399, 417
MboI GATC 2 cut(s) 397, 415
MboII GAAGA 2 cut(s) 418, 623
MhlI GDGCHC 2 cut(s) 33, 220
MluCI AATT 4 cut(s) 332, 356, 464, 735
MlyI GAGTC 1 cut(s) 271
MnlI CCTC 7 cut(s) 88, 152, 188, 607, 702, 751, 776
Mph1103I ATGCAT 1 cut(s) 865
MseI TTAA 3 cut(s) 459, 512, 840
MslI CAYNNNNRTG 1 cut(s) 512
MspA1I CMGCKG 2 cut(s) 403, 542
MspI CCGG 5 cut(s) 53, 100, 148, 236, 260
MspR9I CCNGG 7 cut(s) 54, 100, 101, 148, 237, 260, 380
MvaI CCWGG 1 cut(s) 380
MvnI CGCG 4 cut(s) 191, 329, 542, 850
MwoI GCNNNNNNNGC 6 cut(s) 204, 254, 773, 803, 830, 856
NciI CCSGG 6 cut(s) 54, 100, 101, 148, 237, 260
NcoI CCATGG 2 cut(s) 76, 132
NdeI CATATG 1 cut(s) 373
NdeII GATC 2 cut(s) 397, 415
NlaIV GGNNCC 1 cut(s) 267
NsiI ATGCAT 1 cut(s) 865
NspI RCATGY 2 cut(s) 327, 863
PaeI GCATGC 2 cut(s) 327, 863
PauI GCGCGC 1 cut(s) 327
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PfeI GAWTC 2 cut(s) 66, 271
PflFI GACNNNGTC 1 cut(s) 54
PfoI TCCNGGA 1 cut(s) 146
PkrI GCNGC 3 cut(s) 18, 197, 799
PleI GAGTC 1 cut(s) 271
PmaCI CACGTG 1 cut(s) 215
PmlI CACGTG 1 cut(s) 215
PpsI GAGTC 1 cut(s) 271
Ppu21I YACGTR 1 cut(s) 215
Psp6I CCWGG 1 cut(s) 378
PspCI CACGTG 1 cut(s) 215
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 5 cut(s) 80, 97, 154, 265, 689
PstI CTGCAG 1 cut(s) 802
PstNI CAGNNNCTG 1 cut(s) 806
PsyI GACNNNGTC 1 cut(s) 54
PteI GCGCGC 1 cut(s) 327
PvuII CAGCTG 1 cut(s) 403
RsaI GTAC 6 cut(s) 203, 253, 286, 523, 724, 751
RsaNI GTAC 6 cut(s) 202, 252, 285, 522, 723, 750
RseI CAYNNNNRTG 1 cut(s) 512
SacII CCGCGG 1 cut(s) 543
SalI GTCGAC 2 cut(s) 137, 164
SaqAI TTAA 3 cut(s) 459, 512, 840
SatI GCNGC 3 cut(s) 17, 196, 798
Sau3AI GATC 2 cut(s) 397, 415
Sau96I GGNCC 5 cut(s) 80, 97, 154, 265, 689
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 7 cut(s) 54, 100, 101, 148, 237, 260, 380
SduI GDGCHC 2 cut(s) 33, 220
SfaNI GCATC 2 cut(s) 568, 820
SfcI CTRYAG 1 cut(s) 798
Sfr303I CCGCGG 1 cut(s) 543
SgrBI CCGCGG 1 cut(s) 543
SinI GGWCC 3 cut(s) 80, 265, 689
SmaI CCCGGG 1 cut(s) 101
SmiMI CAYNNNNRTG 1 cut(s) 512
SmlI CTYRAG 2 cut(s) 179, 697
SmoI CTYRAG 2 cut(s) 179, 697
SphI GCATGC 2 cut(s) 327, 863
Sse9I AATT 4 cut(s) 332, 356, 464, 735
SsiI CCGC 8 cut(s) 33, 63, 189, 196, 519, 540, 542, 850
SspI AATATT 1 cut(s) 243
SspMI CTAG 3 cut(s) 320, 384, 731
StyD4I CCNGG 7 cut(s) 52, 98, 99, 146, 235, 258, 378
StyI CCWWGG 3 cut(s) 76, 132, 685
TaaI ACNGT 2 cut(s) 422, 793
TaiI ACGT 2 cut(s) 165, 217
TaqI TCGA 2 cut(s) 138, 165
TasI AATT 4 cut(s) 332, 356, 464, 735
TatI WGTACW 4 cut(s) 251, 284, 722, 749
TauI GCSGC 1 cut(s) 198
TfiI GAWTC 2 cut(s) 66, 271
Tru1I TTAA 3 cut(s) 459, 512, 840
Tru9I TTAA 3 cut(s) 459, 512, 840
TscAI CASTG 3 cut(s) 309, 427, 807
TseI GCWGC 2 cut(s) 16, 797
TspDTI ATGAA 2 cut(s) 496, 517
TspMI CCCGGG 1 cut(s) 99
TspRI CASTG 3 cut(s) 309, 427, 807
Tth111I GACNNNGTC 1 cut(s) 54
VpaK11BI GGWCC 3 cut(s) 80, 265, 689
XapI RAATTY 1 cut(s) 464
XceI RCATGY 2 cut(s) 327, 863
XmaI CCCGGG 1 cut(s) 99
XmiI GTMKAC 4 cut(s) 138, 165, 363, 426
XspI CTAG 3 cut(s) 320, 384, 731
Zsp2I ATGCAT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.