MD08G1134300.v1.1

Fasciclin-like arabinogalactan protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001500
Physical Location & Seq
Forward (+)
12790624 .. 12791475
852 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1134300.v1.1.491

Sequence Viewer

Length: 852 bp
ATGGAGAACACCAAAGCCTCTGTTATCCTCTGCCTTGCCCTCTTCCTTGTCTTCTCTTCGGCCTCGGCCTTTGACATCGTCAAGCTTCTCGAGAAGCAGTCGGAACTGAGCAGCTTCACCAACTACCTCAAGGAGACCAAGCTCGGCGACGAGATCAACAAGCGCAGCACCATCACAGTGTTGGCAGTGGACAATGGGGGCATGTCAAGCATCTCCGGAAAATCCCAAGATGTGATCAAGAAAATCCTCAGCGCACACGTTGTTTTGGATTACTTCGACCAGAAGAAACTGACCAGTTTGGCCGGTTCCAGCAAGGAATCAACCCTAACCACAATGCTCCAGTCCTCTGGCCAGGCCAAGGACCAGCAAGGGTTCATCATTGTCGGTAAGAGCGATGGAGAAATTGCTTTCGGGTCAGCTGTCAAGGGCGGCAAGCTCAACGCCAAGCTCGTGAAAGCCGTTGTCTCCCAGCCCTACAACATTTCAGTTCTTCAAATCTCAAATGCGATTGAAATTCCCGACATTTCTGCTGCCTCTAGGCCTGCCGGCTCGCCAAAGTCCTCCGCATCACCAGCTTCTTCTCCATCAAGCAAATCATCCTCTGATGCTCCCAGTCCCTCTGATGACAGTGCCCCAAGCCAATCACCCAAGTCATCAAAGGCACCCTCGCCATCAGATGAGGTTTCATCCCCACCGGTGTCTGGTGATGCTACACCTTCATCCTCACCATCTAGTGATGGACCAACCGGATCATCAAGCTCTGCACCTTCACCGTCTGCTGCTTCACGTGCTGAGATGGCTTTTGGCGCCGGAGTGGTGATCATGGGTTTGGCTTCCCTTTTGGTCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fasciclin

Protein Analysis

284

Amino Acids

28.72

Weight (kDa)

6.61

Isoelectric Point (pI)

70.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 661, 806
AccIII TCCGGA 1 cut(s) 215
AciI CCGC 2 cut(s) 429, 564
AclWI GGATC 1 cut(s) 757
AcoI YGGCCR 2 cut(s) 300, 349
AcsI RAATTY 1 cut(s) 513
AcvI CACGTG 1 cut(s) 788
AcyI GRCGYC 1 cut(s) 807
AfiI CCNNNNNNNGG 2 cut(s) 358, 701
AflIII ACRYGT 1 cut(s) 256
AgeI ACCGGT 1 cut(s) 694
AgsI TTSAA 2 cut(s) 494, 512
AjnI CCWGG 1 cut(s) 351
AluBI AGCT 8 cut(s) 85, 114, 142, 419, 436, 448, 575, 759
AluI AGCT 8 cut(s) 85, 114, 142, 419, 436, 448, 575, 759
Alw26I GTCTC 2 cut(s) 128, 469
AlwI GGATC 1 cut(s) 757
Ama87I CYCGRG 1 cut(s) 89
Aor13HI TCCGGA 1 cut(s) 215
AoxI GGCC 6 cut(s) 60, 66, 300, 349, 354, 539
ApeKI GCWGC 4 cut(s) 111, 165, 530, 779
ApoI RAATTY 1 cut(s) 513
AsiGI ACCGGT 1 cut(s) 694
AspLEI GCGC 3 cut(s) 165, 254, 809
AspS9I GGNCC 2 cut(s) 361, 740
AsuHPI GGTGA 7 cut(s) 109, 561, 636, 716, 717, 762, 829
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 2 cut(s) 361, 740
BaeGI GKGCMC 1 cut(s) 634
BalI TGGCCA 1 cut(s) 351
BanI GGYRCC 2 cut(s) 661, 806
BauI CACGAG 1 cut(s) 449
BbrPI CACGTG 1 cut(s) 788
BbsI GAAGAC 1 cut(s) 43
BbvCI CCTCAGC 1 cut(s) 248
BbvI GCAGC 4 cut(s) 123, 177, 517, 766
BccI CCATC 7 cut(s) 179, 389, 592, 679, 731, 736, 790
BceAI ACGGC 1 cut(s) 443
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BciT130I CCWGG 1 cut(s) 353
BclI TGATCA 2 cut(s) 234, 819
BcoDI GTCTC 2 cut(s) 128, 469
BfaI CTAG 2 cut(s) 537, 732
BfoI RGCGCY 1 cut(s) 810
BisI GCNGC 5 cut(s) 112, 166, 430, 531, 780
BlsI GCNGC 5 cut(s) 113, 167, 431, 532, 781
Bme1390I CCNGG 1 cut(s) 353
Bme18I GGWCC 2 cut(s) 361, 740
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 2 cut(s) 361, 740
BmiI GGNNCC 3 cut(s) 307, 663, 808
BmrFI CCNGG 1 cut(s) 353
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 4 cut(s) 219, 575, 595, 697
BmuI ACTGGG 1 cut(s) 606
BpiI GAAGAC 1 cut(s) 43
BpmI CTGGAG 1 cut(s) 323
Bpu10I CCTNAGC 1 cut(s) 248
BpuEI CTTGAG 1 cut(s) 113
BsaAI YACGTR 1 cut(s) 788
BsaHI GRCGYC 1 cut(s) 807
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 2 cut(s) 63, 357
BsaWI WCCGGW 3 cut(s) 215, 694, 746
Bsc4I CCNNNNNNNGG 2 cut(s) 358, 701
Bse118I RCCGGY 3 cut(s) 302, 545, 694
Bse1I ACTGG 3 cut(s) 294, 340, 612
BseAI TCCGGA 1 cut(s) 215
BseBI CCWGG 1 cut(s) 353
BseDI CCNNGG 2 cut(s) 63, 357
BseGI GGATG 3 cut(s) 596, 686, 719
BseLI CCNNNNNNNGG 2 cut(s) 358, 701
BseMII CTCAG 3 cut(s) 98, 262, 783
BseNI ACTGG 3 cut(s) 294, 340, 612
BseSI GKGCMC 1 cut(s) 634
BseXI GCAGC 4 cut(s) 123, 177, 517, 766
BseYI CCCAGC 1 cut(s) 468
BsgI GTGCAG 1 cut(s) 747
BshFI GGCC 6 cut(s) 62, 68, 302, 351, 356, 541
BshNI GGYRCC 2 cut(s) 661, 806
BshTI ACCGGT 1 cut(s) 694
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 6 cut(s) 216, 303, 546, 695, 747, 810
BslFI GGGAC 1 cut(s) 600
BslI CCNNNNNNNGG 2 cut(s) 358, 701
BsmAI GTCTC 2 cut(s) 128, 469
BsmFI GGGAC 1 cut(s) 600
BsnI GGCC 6 cut(s) 62, 68, 302, 351, 356, 541
Bso31I GGTCTC 1 cut(s) 128
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 634
Bsp13I TCCGGA 1 cut(s) 215
Bsp143I GATC 4 cut(s) 153, 234, 749, 819
BspACI CCGC 2 cut(s) 429, 564
BspANI GGCC 6 cut(s) 62, 68, 302, 351, 356, 541
BspCNI CTCAG 3 cut(s) 99, 261, 784
BspEI TCCGGA 1 cut(s) 215
BspLI GGNNCC 3 cut(s) 307, 663, 808
BspPI GGATC 1 cut(s) 757
BspT107I GGYRCC 2 cut(s) 661, 806
BspTNI GGTCTC 1 cut(s) 128
BsrFI RCCGGY 3 cut(s) 302, 545, 694
BsrI ACTGG 3 cut(s) 294, 340, 612
BssAI RCCGGY 3 cut(s) 302, 545, 694
BssECI CCNNGG 2 cut(s) 63, 357
BssMI GATC 4 cut(s) 153, 234, 749, 819
BssNI GRCGYC 1 cut(s) 807
BssSI CACGAG 1 cut(s) 449
BssT1I CCWWGG 1 cut(s) 357
Bst2BI CACGAG 1 cut(s) 449
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 3 cut(s) 178, 629, 774
Bst6I CTCTTC 2 cut(s) 47, 61
BstACI GRCGYC 1 cut(s) 807
BstBAI YACGTR 1 cut(s) 788
BstC8I GCNNGC 4 cut(s) 434, 543, 547, 551
BstDEI CTNAG 3 cut(s) 107, 248, 792
BstF5I GGATG 3 cut(s) 596, 686, 719
BstH2I RGCGCY 1 cut(s) 810
BstHHI GCGC 3 cut(s) 165, 254, 809
BstKTI GATC 4 cut(s) 156, 237, 752, 822
BstMAI GTCTC 2 cut(s) 128, 469
BstMBI GATC 4 cut(s) 153, 234, 749, 819
BstMWI GCNNNNNNNGC 5 cut(s) 207, 572, 788, 797, 806
BstNI CCWGG 1 cut(s) 353
BstNSI RCATGY 1 cut(s) 205
BstSCI CCNGG 1 cut(s) 351
BstSLI GKGCMC 1 cut(s) 634
BstV1I GCAGC 4 cut(s) 123, 177, 517, 766
BstV2I GAAGAC 1 cut(s) 43
BstXI CCANNNNNNTGG 1 cut(s) 347
BsuRI GGCC 6 cut(s) 62, 68, 302, 351, 356, 541
BtgZI GCGATG 1 cut(s) 408
BtsCI GGATG 3 cut(s) 596, 686, 719
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 3 cut(s) 183, 192, 634
Cac8I GCNNGC 4 cut(s) 434, 543, 547, 551
CfoI GCGC 3 cut(s) 165, 254, 809
Cfr10I RCCGGY 3 cut(s) 302, 545, 694
Cfr13I GGNCC 2 cut(s) 361, 740
CspAI ACCGGT 1 cut(s) 694
CviAII CATG 2 cut(s) 202, 823
DdeI CTNAG 3 cut(s) 107, 248, 792
DinI GGCGCC 1 cut(s) 808
DpnI GATC 4 cut(s) 155, 236, 751, 821
DpnII GATC 4 cut(s) 153, 234, 749, 819
EaeI YGGCCR 2 cut(s) 300, 349
Eam1104I CTCTTC 2 cut(s) 47, 61
EarI CTCTTC 2 cut(s) 47, 61
Eco130I CCWWGG 1 cut(s) 357
Eco147I AGGCCT 1 cut(s) 541
Eco31I GGTCTC 1 cut(s) 128
Eco47I GGWCC 2 cut(s) 361, 740
Eco72I CACGTG 1 cut(s) 788
Eco88I CYCGRG 1 cut(s) 89
EcoRII CCWGG 1 cut(s) 351
EcoT14I CCWWGG 1 cut(s) 357
EgeI GGCGCC 1 cut(s) 808
EheI GGCGCC 1 cut(s) 808
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 2 cut(s) 205, 826
FaiI YATR 2 cut(s) 203, 824
FaqI GGGAC 1 cut(s) 600
FatI CATG 2 cut(s) 201, 822
FbaI TGATCA 2 cut(s) 234, 819
Fnu4HI GCNGC 5 cut(s) 112, 166, 430, 531, 780
FokI GGATG 3 cut(s) 583, 673, 706
Fsp4HI GCNGC 5 cut(s) 112, 166, 430, 531, 780
FspBI CTAG 2 cut(s) 537, 732
GlaI GCGC 3 cut(s) 164, 253, 808
GluI GCNGC 5 cut(s) 112, 166, 430, 531, 780
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 1 cut(s) 323
HaeII RGCGCY 1 cut(s) 810
HaeIII GGCC 6 cut(s) 62, 68, 302, 351, 356, 541
HapII CCGG 6 cut(s) 216, 303, 546, 695, 747, 810
HhaI GCGC 3 cut(s) 165, 254, 809
Hin1I GRCGYC 1 cut(s) 807
Hin1II CATG 2 cut(s) 205, 826
Hin6I GCGC 3 cut(s) 163, 252, 807
HinP1I GCGC 3 cut(s) 163, 252, 807
HindIII AAGCTT 1 cut(s) 83
HinfI GANTC 1 cut(s) 317
HpaII CCGG 6 cut(s) 216, 303, 546, 695, 747, 810
HphI GGTGA 7 cut(s) 109, 561, 636, 716, 717, 762, 829
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 4 cut(s) 103, 604, 622, 676
Hpy188III TCNNGA 6 cut(s) 89, 91, 216, 238, 451, 518
Hpy8I GTNNAC 1 cut(s) 190
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 2 cut(s) 726, 777
HpyCH4III ACNGT 3 cut(s) 178, 629, 774
HpyCH4IV ACGT 2 cut(s) 258, 787
HpyCH4V TGCA 1 cut(s) 764
HpyF10VI GCNNNNNNNGC 5 cut(s) 207, 572, 788, 797, 806
HpyF3I CTNAG 3 cut(s) 107, 248, 792
HpySE526I ACGT 2 cut(s) 258, 787
Hsp92I GRCGYC 1 cut(s) 807
Hsp92II CATG 2 cut(s) 205, 826
HspAI GCGC 3 cut(s) 163, 252, 807
KasI GGCGCC 1 cut(s) 806
Kpn2I TCCGGA 1 cut(s) 215
KroI GCCGGC 1 cut(s) 545
KroNI GCCGGC 1 cut(s) 547
Ksp22I TGATCA 2 cut(s) 234, 819
Kzo9I GATC 4 cut(s) 153, 234, 749, 819
LmnI GCTCC 2 cut(s) 342, 613
Lsp1109I GCAGC 4 cut(s) 123, 177, 517, 766
LweI GCATC 4 cut(s) 219, 575, 595, 697
MaeI CTAG 2 cut(s) 537, 732
MaeII ACGT 2 cut(s) 258, 787
MalI GATC 4 cut(s) 155, 236, 751, 821
MboI GATC 4 cut(s) 153, 234, 749, 819
MboII GAAGA 6 cut(s) 34, 43, 48, 295, 482, 570
MhlI GDGCHC 1 cut(s) 634
MlsI TGGCCA 1 cut(s) 351
MluCI AATT 2 cut(s) 402, 513
MluNI TGGCCA 1 cut(s) 351
Mly113I GGCGCC 1 cut(s) 807
MmeI TCCRAC 1 cut(s) 81
Mox20I TGGCCA 1 cut(s) 351
MroI TCCGGA 1 cut(s) 215
MroNI GCCGGC 1 cut(s) 545
MscI TGGCCA 1 cut(s) 351
MseI TTAA 1 cut(s) 850
MslI CAYNNNNRTG 1 cut(s) 176
Msp20I TGGCCA 1 cut(s) 351
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 6 cut(s) 216, 303, 546, 695, 747, 810
MspR9I CCNGG 1 cut(s) 353
MvaI CCWGG 1 cut(s) 353
MwoI GCNNNNNNNGC 5 cut(s) 207, 572, 788, 797, 806
NaeI GCCGGC 1 cut(s) 547
NarI GGCGCC 1 cut(s) 807
NdeII GATC 4 cut(s) 153, 234, 749, 819
NgoMIV GCCGGC 1 cut(s) 545
NlaIII CATG 2 cut(s) 205, 826
NlaIV GGNNCC 3 cut(s) 307, 663, 808
NmeAIII GCCGAG 2 cut(s) 44, 123
NspI RCATGY 1 cut(s) 205
PaeR7I CTCGAG 1 cut(s) 89
PceI AGGCCT 1 cut(s) 541
PcsI WCGNNNNNNNCGW 3 cut(s) 390, 447, 456
PdiI GCCGGC 1 cut(s) 547
PfeI GAWTC 1 cut(s) 317
PflFI GACNNNGTC 1 cut(s) 77
PinAI ACCGGT 1 cut(s) 694
PkrI GCNGC 5 cut(s) 113, 167, 431, 532, 781
PluTI GGCGCC 1 cut(s) 810
PmaCI CACGTG 1 cut(s) 788
PmlI CACGTG 1 cut(s) 788
Ppu21I YACGTR 1 cut(s) 788
Psp6I CCWGG 1 cut(s) 351
PspCI CACGTG 1 cut(s) 788
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 3 cut(s) 307, 663, 808
PspPI GGNCC 2 cut(s) 361, 740
PsyI GACNNNGTC 1 cut(s) 77
PvuII CAGCTG 1 cut(s) 419
RseI CAYNNNNRTG 1 cut(s) 176
SaqAI TTAA 1 cut(s) 850
SatI GCNGC 5 cut(s) 112, 166, 430, 531, 780
Sau3AI GATC 4 cut(s) 153, 234, 749, 819
Sau96I GGNCC 2 cut(s) 361, 740
ScrFI CCNGG 1 cut(s) 353
SduI GDGCHC 1 cut(s) 634
SfaNI GCATC 4 cut(s) 219, 575, 595, 697
SfoI GGCGCC 1 cut(s) 808
Sfr274I CTCGAG 1 cut(s) 89
SgrAI CRCCGGYG 1 cut(s) 694
SinI GGWCC 2 cut(s) 361, 740
SlaI CTCGAG 1 cut(s) 89
SmiMI CAYNNNNRTG 1 cut(s) 176
SmlI CTYRAG 2 cut(s) 89, 128
SmoI CTYRAG 2 cut(s) 89, 128
Sse9I AATT 2 cut(s) 402, 513
SseBI AGGCCT 1 cut(s) 541
SsiI CCGC 2 cut(s) 429, 564
SspDI GGCGCC 1 cut(s) 806
SspMI CTAG 2 cut(s) 537, 732
StuI AGGCCT 1 cut(s) 541
StyD4I CCNGG 1 cut(s) 351
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 3 cut(s) 178, 629, 774
TaiI ACGT 2 cut(s) 261, 790
TaqI TCGA 2 cut(s) 90, 276
TasI AATT 2 cut(s) 402, 513
TauI GCSGC 1 cut(s) 432
TfiI GAWTC 1 cut(s) 317
Tru1I TTAA 1 cut(s) 850
Tru9I TTAA 1 cut(s) 850
TscAI CASTG 3 cut(s) 183, 192, 634
TseI GCWGC 4 cut(s) 111, 165, 530, 779
TspDTI ATGAA 3 cut(s) 364, 675, 708
TspRI CASTG 3 cut(s) 183, 192, 634
Tth111I GACNNNGTC 1 cut(s) 77
VpaK11BI GGWCC 2 cut(s) 361, 740
XapI RAATTY 1 cut(s) 513
XceI RCATGY 1 cut(s) 205
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XhoI CTCGAG 1 cut(s) 89
XspI CTAG 2 cut(s) 537, 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.