MD08G1176500.v1.1

Fantastic Four meristem regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002468
Physical Location & Seq
Reverse (-)
21411692 .. 21412306
615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1176500.v1.1.491

Sequence Viewer

Length: 615 bp
ATGACGCTCTCTGACTTGCTATCCCCAACTTCCTCTTCCCTTTTGACCGGGGACTACATTGGAATGGAGAGCTCGGTTGACGTGCTCAAGGACTGTGATGATCATAAACCGCGACTGAAGGTGACCGGTAGCGGTGGTGATGGGCATGATGGTAGTTTTGGTCAGAGGGTAAGGAGTAGGAGAGAGCAAAAATGGGCATTGAAGAACAAGGAGTTTCCACCTCCTATACAATTGCTAGCACGCACTGAGAACTTGCCTTCTCATATGCCTTGGGTGTTGAAGAGGCATTACACAGATGATGGGAGGTTGATTCTCACCGAGGAGAAGGTTAAGCATCATGAGTACTTTAGTGCTCATAGGTCTAATGGCAGGCTCACTTTGCAGCTTGTCCTGCTTGATGATGAGATTTTGATTCCCCCATTTGCTTGCAATGGGAACGAGAATGAGAACGAGAACGAGAACGGGGACATGAACAAGAATGAGATCGGGAATGATGTTGGTGATGGTGGCCATGACAACAACGATGATGATGGTTGTGAAGATGATGACGAGGATGATGTTGTTGAAGATCAAAAAATTAGTGGGATTGCGGGATCAGGAGCTTCAGCGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAF

Protein Analysis

205

Amino Acids

22.48

Weight (kDa)

4.59

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAF PF11250 71 - 129 2.2e-20 Fantastic Four meristem regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 112
AciI CCGC 4 cut(s) 110, 132, 590, 608
AclWI GGATC 1 cut(s) 601
AcoI YGGCCR 1 cut(s) 508
AcuI CTGAAG 2 cut(s) 137, 588
AfaI GTAC 1 cut(s) 344
AgeI ACCGGT 1 cut(s) 125
AgsI TTSAA 3 cut(s) 202, 280, 566
AjiI CACGTC 1 cut(s) 82
AjuI GAANNNNNNNTTGG 2 cut(s) 19, 51
AluBI AGCT 3 cut(s) 72, 385, 602
AluI AGCT 3 cut(s) 72, 385, 602
Alw21I GWGCWC 3 cut(s) 74, 87, 355
AlwI GGATC 1 cut(s) 601
AoxI GGCC 1 cut(s) 508
ApeKI GCWGC 1 cut(s) 382
AsiGI ACCGGT 1 cut(s) 125
AsuC2I CCSGG 1 cut(s) 49
AsuHPI GGTGA 4 cut(s) 133, 149, 307, 512
AsuNHI GCTAGC 1 cut(s) 235
BalI TGGCCA 1 cut(s) 510
BanII GRGCYC 1 cut(s) 74
BarI GAAGNNNNNNTAC 2 cut(s) 272, 304
Bbv12I GWGCWC 3 cut(s) 74, 87, 355
BbvI GCAGC 1 cut(s) 394
BccI CCATC 5 cut(s) 134, 143, 293, 497, 524
BclI TGATCA 1 cut(s) 100
BcnI CCSGG 1 cut(s) 49
BfaI CTAG 1 cut(s) 236
BisI GCNGC 1 cut(s) 383
BlsI GCNGC 1 cut(s) 384
BmcAI AGTACT 1 cut(s) 344
Bme1390I CCNGG 1 cut(s) 49
BmgBI CACGTC 1 cut(s) 82
BmrFI CCNGG 1 cut(s) 49
BmsI GCATC 1 cut(s) 343
BmtI GCTAGC 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 71
BpuMI CCSGG 1 cut(s) 49
BsaJI CCNNGG 3 cut(s) 48, 269, 318
BsaWI WCCGGW 1 cut(s) 125
Bse118I RCCGGY 1 cut(s) 125
Bse3DI GCAATG 1 cut(s) 436
BseDI CCNNGG 3 cut(s) 48, 269, 318
BseGI GGATG 1 cut(s) 559
BseMI GCAATG 1 cut(s) 436
BseMII CTCAG 1 cut(s) 237
BseRI GAGGAG 1 cut(s) 335
BseXI GCAGC 1 cut(s) 394
Bsh1236I CGCG 1 cut(s) 112
BshFI GGCC 1 cut(s) 510
BshTI ACCGGT 1 cut(s) 125
BsiHKAI GWGCWC 3 cut(s) 74, 87, 355
BsiSI CCGG 2 cut(s) 48, 126
BslFI GGGAC 2 cut(s) 65, 479
BsmFI GGGAC 2 cut(s) 65, 479
BsnI GGCC 1 cut(s) 510
Bsp1286I GDGCHC 3 cut(s) 74, 87, 355
Bsp143I GATC 4 cut(s) 100, 483, 568, 593
BspACI CCGC 4 cut(s) 110, 132, 590, 608
BspANI GGCC 1 cut(s) 510
BspCNI CTCAG 1 cut(s) 238
BspFNI CGCG 1 cut(s) 112
BspHI TCATGA 1 cut(s) 337
BspOI GCTAGC 1 cut(s) 239
BspPI GGATC 1 cut(s) 601
BsrDI GCAATG 1 cut(s) 436
BsrFI RCCGGY 1 cut(s) 125
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 3 cut(s) 48, 269, 318
BssMI GATC 4 cut(s) 100, 483, 568, 593
BssT1I CCWWGG 1 cut(s) 269
Bst4CI ACNGT 1 cut(s) 95
Bst6I CTCTTC 2 cut(s) 40, 275
BstC8I GCNNGC 4 cut(s) 237, 241, 371, 427
BstDEI CTNAG 1 cut(s) 246
BstEII GGTNACC 1 cut(s) 121
BstF5I GGATG 1 cut(s) 559
BstFNI CGCG 1 cut(s) 112
BstKTI GATC 4 cut(s) 103, 486, 571, 596
BstMBI GATC 4 cut(s) 100, 483, 568, 593
BstMWI GCNNNNNNNGC 2 cut(s) 379, 391
BstPI GGTNACC 1 cut(s) 121
BstSCI CCNGG 1 cut(s) 47
BstUI CGCG 1 cut(s) 112
BstV1I GCAGC 1 cut(s) 394
BsuRI GGCC 1 cut(s) 510
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 1 cut(s) 559
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 4 cut(s) 237, 241, 371, 427
CciI TCATGA 1 cut(s) 337
Cfr10I RCCGGY 1 cut(s) 125
CseI GACGC 1 cut(s) 13
Csp6I GTAC 1 cut(s) 343
CspAI ACCGGT 1 cut(s) 125
CviAII CATG 4 cut(s) 146, 338, 469, 512
CviJI RGCY 5 cut(s) 72, 373, 385, 510, 602
CviKI_1 RGCY 5 cut(s) 72, 373, 385, 510, 602
CviQI GTAC 1 cut(s) 343
DdeI CTNAG 1 cut(s) 246
DpnI GATC 4 cut(s) 102, 485, 570, 595
DpnII GATC 4 cut(s) 100, 483, 568, 593
EaeI YGGCCR 1 cut(s) 508
Eam1104I CTCTTC 2 cut(s) 40, 275
EarI CTCTTC 2 cut(s) 40, 275
Ecl136II GAGCTC 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 269
Eco24I GRGCYC 1 cut(s) 74
Eco53kI GAGCTC 1 cut(s) 72
Eco57I CTGAAG 2 cut(s) 137, 588
Eco91I GGTNACC 1 cut(s) 121
EcoICRI GAGCTC 1 cut(s) 72
EcoO65I GGTNACC 1 cut(s) 121
EcoT14I CCWWGG 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 74
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 4 cut(s) 149, 341, 472, 515
FaiI YATR 9 cut(s) 105, 147, 227, 264, 266, 339, 357, 470, 513
FaqI GGGAC 2 cut(s) 65, 479
FatI CATG 4 cut(s) 145, 337, 468, 511
FauI CCCGC 2 cut(s) 583, 601
FauNDI CATATG 1 cut(s) 264
FbaI TGATCA 1 cut(s) 100
Fnu4HI GCNGC 1 cut(s) 383
FokI GGATG 1 cut(s) 566
FriOI GRGCYC 1 cut(s) 74
Fsp4HI GCNGC 1 cut(s) 383
FspBI CTAG 1 cut(s) 236
GluI GCNGC 1 cut(s) 383
HaeIII GGCC 1 cut(s) 510
HapII CCGG 2 cut(s) 48, 126
HgaI GACGC 1 cut(s) 13
Hin1II CATG 4 cut(s) 149, 341, 472, 515
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 2 cut(s) 310, 412
HpaII CCGG 2 cut(s) 48, 126
HphI GGTGA 4 cut(s) 133, 149, 307, 512
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 2 cut(s) 13, 165
Hpy188III TCNNGA 3 cut(s) 338, 487, 597
Hpy8I GTNNAC 1 cut(s) 79
HpyAV CCTTC 3 cut(s) 112, 267, 319
HpyCH4III ACNGT 1 cut(s) 95
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 2 cut(s) 382, 429
HpyF10VI GCNNNNNNNGC 2 cut(s) 379, 391
HpyF3I CTNAG 1 cut(s) 246
HpySE526I ACGT 1 cut(s) 81
Hsp92II CATG 4 cut(s) 149, 341, 472, 515
Ksp22I TGATCA 1 cut(s) 100
Kzo9I GATC 4 cut(s) 100, 483, 568, 593
LmnI GCTCC 1 cut(s) 599
LpnPI CCDG 5 cut(s) 61, 139, 355, 404, 582
Lsp1109I GCAGC 1 cut(s) 394
LweI GCATC 1 cut(s) 343
MaeI CTAG 1 cut(s) 236
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 1 cut(s) 121
MalI GATC 4 cut(s) 102, 485, 570, 595
MboI GATC 4 cut(s) 100, 483, 568, 593
MboII GAAGA 5 cut(s) 27, 214, 292, 551, 578
MfeI CAATTG 1 cut(s) 230
MhlI GDGCHC 3 cut(s) 74, 87, 355
MlsI TGGCCA 1 cut(s) 510
MluCI AATT 2 cut(s) 230, 576
MluNI TGGCCA 1 cut(s) 510
MnlI CCTC 7 cut(s) 43, 159, 231, 276, 297, 313, 544
Mox20I TGGCCA 1 cut(s) 510
MscI TGGCCA 1 cut(s) 510
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 62
Msp20I TGGCCA 1 cut(s) 510
MspA1I CMGCKG 1 cut(s) 608
MspI CCGG 2 cut(s) 48, 126
MspR9I CCNGG 1 cut(s) 49
MunI CAATTG 1 cut(s) 230
MvnI CGCG 1 cut(s) 112
MwoI GCNNNNNNNGC 2 cut(s) 379, 391
NciI CCSGG 1 cut(s) 49
NdeI CATATG 1 cut(s) 264
NdeII GATC 4 cut(s) 100, 483, 568, 593
NheI GCTAGC 1 cut(s) 235
NlaIII CATG 4 cut(s) 149, 341, 472, 515
NmuCI GTSAC 1 cut(s) 121
PagI TCATGA 1 cut(s) 337
PfeI GAWTC 2 cut(s) 310, 412
PinAI ACCGGT 1 cut(s) 125
PkrI GCNGC 1 cut(s) 384
Psp124BI GAGCTC 1 cut(s) 74
PspEI GGTNACC 1 cut(s) 121
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
RseI CAYNNNNRTG 1 cut(s) 62
SacI GAGCTC 1 cut(s) 74
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 1 cut(s) 383
Sau3AI GATC 4 cut(s) 100, 483, 568, 593
ScaI AGTACT 1 cut(s) 344
ScrFI CCNGG 1 cut(s) 49
SduI GDGCHC 3 cut(s) 74, 87, 355
SetI ASST 9 cut(s) 74, 84, 123, 223, 308, 330, 362, 387, 604
SfaNI GCATC 1 cut(s) 343
SmiMI CAYNNNNRTG 1 cut(s) 62
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 2 cut(s) 230, 576
SsiI CCGC 4 cut(s) 110, 132, 590, 608
SspMI CTAG 1 cut(s) 236
SstI GAGCTC 1 cut(s) 74
StyD4I CCNGG 1 cut(s) 47
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 1 cut(s) 95
TaiI ACGT 1 cut(s) 84
TasI AATT 2 cut(s) 230, 576
TatI WGTACW 1 cut(s) 342
TfiI GAWTC 2 cut(s) 310, 412
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 1 cut(s) 121
TseI GCWGC 1 cut(s) 382
Tsp45I GTSAC 1 cut(s) 121
TspDTI ATGAA 1 cut(s) 485
TspRI CASTG 1 cut(s) 250
XspI CTAG 1 cut(s) 236
ZrmI AGTACT 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.