MD09G1099500.v1.1

Zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001915
Physical Location & Seq
Reverse (-)
7247347 .. 7248629
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1099500.v1.1.491

Sequence Viewer

Length: 693 bp
ATGAAGAATTGCGAGCTCTGCCGGCTTCCCGCGAGAATCTTCTGCGAATCGGACCAGGCGATTCTGTGCTGGGACTGCGACGCCAAGGTTCACGGCGCCAATTTCCTGGTGTCTCGCCACTCCCGGAGCCTCCTCTGCCACGGCTGCCAGTCTCCCACGCCGTGGAAGGCCTCCGGCGAAAAGCTCGGCCACACTTTTTCGGTGTGCGAGAGCTGCGTTCTCCCAGACGGAGGCAGAGACGACGACGACGAGAGCCAGGGCGGCAATGATGAGGAATTCGATTCGGATAACGATGAGATTGACGACGACGATGACGACGACGACGATGAGGATGTTGATTTCGATGAGGACGGGGATAATCAGGTGGTGCCGTTGTCTTCGACGGCGACGCCGCCTCCGCCGACTTCAAGCGCATCGAGCAGCGAGCAGTCTGCGACTGCTTTCAACAATGGTGATGGAGGTGATTCCAAAACCGCCACCGCCGTTACGTTGAAGCGATTCAGAGAAACCGCGTCGGATCTTCGCTCCCAGATCGCCGATCTCGACCGCTCATGTCCTCACCAGAGGTGTGGCTCCGCTAAGGCTGCTCGAGCGGGTCCGTCGGAACGCAACGGCGGAGCCACTTCGGTTGACTCATCGAGACCTCAAAAAGACCGGAGAATCGACCTAAACCGGCCGGCTTCTCCGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-B_box

Protein Analysis

231

Amino Acids

24.73

Weight (kDa)

4.37

Isoelectric Point (pI)

61.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 2 - 42 1.3e-06 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 95, 367
AccB7I CCANNNNNTGG 1 cut(s) 162
AccBSI CCGCTC 2 cut(s) 549, 593
AccII CGCG 2 cut(s) 32, 512
AclWI GGATC 1 cut(s) 525
AcoI YGGCCR 2 cut(s) 187, 674
AcsI RAATTY 1 cut(s) 275
AcyI GRCGYC 3 cut(s) 81, 96, 389
AdeI CACNNNGTG 1 cut(s) 162
AfiI CCNNNNNNNGG 2 cut(s) 162, 230
AgsI TTSAA 3 cut(s) 408, 445, 493
AjnI CCWGG 3 cut(s) 54, 105, 255
AluBI AGCT 3 cut(s) 16, 184, 213
AluI AGCT 3 cut(s) 16, 184, 213
Alw21I GWGCWC 1 cut(s) 18
Alw26I GTCTC 4 cut(s) 117, 156, 231, 634
AlwI GGATC 1 cut(s) 525
Ama87I CYCGRG 1 cut(s) 588
AoxI GGCC 3 cut(s) 168, 187, 674
ApeKI GCWGC 4 cut(s) 144, 213, 420, 584
ApoI RAATTY 1 cut(s) 275
Asp700I GAANNNNTTC 1 cut(s) 497
AspLEI GCGC 2 cut(s) 98, 413
AspS9I GGNCC 2 cut(s) 52, 596
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 3 cut(s) 464, 473, 551
AvaI CYCGRG 1 cut(s) 588
AvaII GGWCC 2 cut(s) 52, 596
BanI GGYRCC 2 cut(s) 95, 367
BanII GRGCYC 1 cut(s) 18
BbsI GAAGAC 1 cut(s) 369
Bbv12I GWGCWC 1 cut(s) 18
BbvI GCAGC 4 cut(s) 131, 200, 432, 571
BccI CCATC 1 cut(s) 449
BceAI ACGGC 7 cut(s) 109, 145, 157, 355, 399, 467, 628
BcgI CGANNNNNNTGC 2 cut(s) 413, 447
BciT130I CCWGG 3 cut(s) 56, 107, 257
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 4 cut(s) 117, 156, 231, 634
BfaI CTAG 1 cut(s) 691
BfoI RGCGCY 1 cut(s) 99
BglI GCCNNNNNGGC 1 cut(s) 261
BisI GCNGC 6 cut(s) 145, 214, 262, 392, 421, 585
BlsI GCNGC 6 cut(s) 146, 215, 263, 393, 422, 586
Bme1390I CCNGG 4 cut(s) 56, 107, 124, 257
Bme18I GGWCC 2 cut(s) 52, 596
BmeT110I CYCGRG 1 cut(s) 588
BmgT120I GGNCC 2 cut(s) 52, 596
BmiI GGNNCC 6 cut(s) 97, 128, 369, 574, 597, 619
BmrFI CCNGG 4 cut(s) 56, 107, 124, 257
BmsI GCATC 1 cut(s) 422
BpiI GAAGAC 1 cut(s) 369
Bpu10I CCTNAGC 1 cut(s) 579
BpuMI CCSGG 1 cut(s) 124
BsaHI GRCGYC 3 cut(s) 81, 96, 389
BsaI GGTCTC 1 cut(s) 634
BsaJI CCNNGG 4 cut(s) 84, 139, 161, 256
BsaWI WCCGGW 1 cut(s) 654
BsaXI ACNNNNNCTCC 2 cut(s) 379, 409
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 230
Bse118I RCCGGY 3 cut(s) 21, 672, 676
Bse1I ACTGG 1 cut(s) 148
Bse3DI GCAATG 1 cut(s) 271
BseBI CCWGG 3 cut(s) 56, 107, 257
BseDI CCNNGG 4 cut(s) 84, 139, 161, 256
BseGI GGATG 1 cut(s) 337
BseLI CCNNNNNNNGG 2 cut(s) 162, 230
BseMI GCAATG 1 cut(s) 271
BseNI ACTGG 1 cut(s) 148
BseRI GAGGAG 1 cut(s) 122
BseX3I CGGCCG 1 cut(s) 674
BseXI GCAGC 4 cut(s) 131, 200, 432, 571
BseYI CCCAGC 1 cut(s) 69
Bsh1236I CGCG 2 cut(s) 32, 512
Bsh1285I CGRYCG 2 cut(s) 547, 677
BshFI GGCC 3 cut(s) 170, 189, 676
BshNI GGYRCC 2 cut(s) 95, 367
BsiEI CGRYCG 2 cut(s) 547, 677
BsiHKAI GWGCWC 1 cut(s) 18
BsiHKCI CYCGRG 1 cut(s) 588
BsiSI CCGG 6 cut(s) 22, 124, 174, 655, 673, 677
BslFI GGGAC 1 cut(s) 86
BslI CCNNNNNNNGG 2 cut(s) 162, 230
BsmAI GTCTC 4 cut(s) 117, 156, 231, 634
BsmBI CGTCTC 1 cut(s) 231
BsmFI GGGAC 1 cut(s) 86
BsnI GGCC 3 cut(s) 170, 189, 676
Bso31I GGTCTC 1 cut(s) 634
BsoBI CYCGRG 1 cut(s) 588
Bsp1286I GDGCHC 1 cut(s) 18
Bsp143I GATC 3 cut(s) 517, 531, 538
BspANI GGCC 3 cut(s) 170, 189, 676
BspFNI CGCG 2 cut(s) 32, 512
BspLI GGNNCC 6 cut(s) 97, 128, 369, 574, 597, 619
BspPI GGATC 1 cut(s) 525
BspT107I GGYRCC 2 cut(s) 95, 367
BspTNI GGTCTC 1 cut(s) 634
BsrBI CCGCTC 2 cut(s) 549, 593
BsrDI GCAATG 1 cut(s) 271
BsrFI RCCGGY 3 cut(s) 21, 672, 676
BsrI ACTGG 1 cut(s) 148
BssAI RCCGGY 3 cut(s) 21, 672, 676
BssECI CCNNGG 4 cut(s) 84, 139, 161, 256
BssMI GATC 3 cut(s) 517, 531, 538
BssNI GRCGYC 3 cut(s) 81, 96, 389
BssT1I CCWWGG 1 cut(s) 84
Bst2UI CCWGG 3 cut(s) 56, 107, 257
BstACI GRCGYC 3 cut(s) 81, 96, 389
BstC8I GCNNGC 4 cut(s) 14, 23, 425, 678
BstDEI CTNAG 1 cut(s) 579
BstDSI CCRYGG 2 cut(s) 139, 161
BstF5I GGATG 1 cut(s) 337
BstFNI CGCG 2 cut(s) 32, 512
BstH2I RGCGCY 1 cut(s) 99
BstHHI GCGC 2 cut(s) 98, 413
BstKTI GATC 3 cut(s) 520, 534, 541
BstMAI GTCTC 4 cut(s) 117, 156, 231, 634
BstMBI GATC 3 cut(s) 517, 531, 538
BstMCI CGRYCG 2 cut(s) 547, 677
BstNI CCWGG 3 cut(s) 56, 107, 257
BstSCI CCNGG 4 cut(s) 54, 105, 122, 255
BstUI CGCG 2 cut(s) 32, 512
BstV1I GCAGC 4 cut(s) 131, 200, 432, 571
BstV2I GAAGAC 1 cut(s) 369
BstX2I RGATCY 1 cut(s) 517
BstXI CCANNNNNNTGG 2 cut(s) 106, 569
BstYI RGATCY 1 cut(s) 517
BstZI CGGCCG 1 cut(s) 674
BsuRI GGCC 3 cut(s) 170, 189, 676
BtgI CCRYGG 2 cut(s) 139, 161
BtsCI GGATG 1 cut(s) 337
Cac8I GCNNGC 4 cut(s) 14, 23, 425, 678
CfoI GCGC 2 cut(s) 98, 413
Cfr10I RCCGGY 3 cut(s) 21, 672, 676
Cfr13I GGNCC 2 cut(s) 52, 596
CseI GACGC 3 cut(s) 89, 397, 501
CspCI CAANNNNNGTGG 2 cut(s) 610, 645
CviAII CATG 1 cut(s) 552
DdeI CTNAG 1 cut(s) 579
DinI GGCGCC 1 cut(s) 97
DpnI GATC 3 cut(s) 519, 533, 540
DpnII GATC 3 cut(s) 517, 531, 538
DraIII CACNNNGTG 1 cut(s) 162
EaeI YGGCCR 2 cut(s) 187, 674
EagI CGGCCG 1 cut(s) 674
EciI GGCGGA 2 cut(s) 387, 630
Ecl136II GAGCTC 1 cut(s) 16
EclXI CGGCCG 1 cut(s) 674
Eco130I CCWWGG 1 cut(s) 84
Eco147I AGGCCT 1 cut(s) 170
Eco24I GRGCYC 1 cut(s) 18
Eco31I GGTCTC 1 cut(s) 634
Eco47I GGWCC 2 cut(s) 52, 596
Eco52I CGGCCG 1 cut(s) 674
Eco53kI GAGCTC 1 cut(s) 16
Eco88I CYCGRG 1 cut(s) 588
EcoICRI GAGCTC 1 cut(s) 16
EcoRI GAATTC 1 cut(s) 275
EcoRII CCWGG 3 cut(s) 54, 105, 255
EcoT14I CCWWGG 1 cut(s) 84
EcoT38I GRGCYC 1 cut(s) 18
EgeI GGCGCC 1 cut(s) 97
EheI GGCGCC 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 84
Esp3I CGTCTC 1 cut(s) 231
FaeI CATG 1 cut(s) 555
FaiI YATR 1 cut(s) 553
FaqI GGGAC 1 cut(s) 86
FatI CATG 1 cut(s) 551
FauI CCCGC 2 cut(s) 37, 586
Fnu4HI GCNGC 6 cut(s) 145, 214, 262, 392, 421, 585
FokI GGATG 1 cut(s) 344
FriOI GRGCYC 1 cut(s) 18
Fsp4HI GCNGC 6 cut(s) 145, 214, 262, 392, 421, 585
FspBI CTAG 1 cut(s) 691
GlaI GCGC 2 cut(s) 97, 412
GluI GCNGC 6 cut(s) 145, 214, 262, 392, 421, 585
GsaI CCCAGC 1 cut(s) 73
HaeII RGCGCY 1 cut(s) 99
HaeIII GGCC 3 cut(s) 170, 189, 676
HapII CCGG 6 cut(s) 22, 124, 174, 655, 673, 677
HgaI GACGC 3 cut(s) 89, 397, 501
HhaI GCGC 2 cut(s) 98, 413
Hin1I GRCGYC 3 cut(s) 81, 96, 389
Hin1II CATG 1 cut(s) 555
Hin6I GCGC 2 cut(s) 96, 411
HinP1I GCGC 2 cut(s) 96, 411
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HinfI GANTC 8 cut(s) 36, 47, 61, 281, 464, 498, 632, 660
HpaII CCGG 6 cut(s) 22, 124, 174, 655, 673, 677
HphI GGTGA 3 cut(s) 464, 473, 551
Hpy166II GTNNAC 2 cut(s) 91, 631
Hpy188I TCNGA 5 cut(s) 52, 286, 503, 517, 604
Hpy188III TCNNGA 2 cut(s) 542, 639
Hpy8I GTNNAC 2 cut(s) 91, 631
HpyAV CCTTC 1 cut(s) 160
HpyCH4IV ACGT 1 cut(s) 488
HpyF3I CTNAG 1 cut(s) 579
HpySE526I ACGT 1 cut(s) 488
Hsp92I GRCGYC 3 cut(s) 81, 96, 389
Hsp92II CATG 1 cut(s) 555
HspAI GCGC 2 cut(s) 96, 411
KasI GGCGCC 1 cut(s) 95
KroI GCCGGC 2 cut(s) 21, 676
KroNI GCCGGC 2 cut(s) 23, 678
Kzo9I GATC 3 cut(s) 517, 531, 538
LmnI GCTCC 4 cut(s) 126, 530, 578, 617
Lsp1109I GCAGC 4 cut(s) 131, 200, 432, 571
LweI GCATC 1 cut(s) 422
MaeI CTAG 1 cut(s) 691
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 1 cut(s) 484
MalI GATC 3 cut(s) 519, 533, 540
MbiI CCGCTC 2 cut(s) 549, 593
MboI GATC 3 cut(s) 517, 531, 538
MboII GAAGA 4 cut(s) 16, 31, 369, 512
MflI RGATCY 1 cut(s) 517
MhlI GDGCHC 1 cut(s) 18
MluCI AATT 3 cut(s) 7, 100, 275
Mly113I GGCGCC 1 cut(s) 96
MlyI GAGTC 1 cut(s) 626
MmeI TCCRAC 2 cut(s) 495, 582
MroNI GCCGGC 2 cut(s) 21, 676
MroXI GAANNNNTTC 1 cut(s) 497
MspI CCGG 6 cut(s) 22, 124, 174, 655, 673, 677
MspR9I CCNGG 4 cut(s) 56, 107, 124, 257
MvaI CCWGG 3 cut(s) 56, 107, 257
MvnI CGCG 2 cut(s) 32, 512
NaeI GCCGGC 2 cut(s) 23, 678
NarI GGCGCC 1 cut(s) 96
NciI CCSGG 1 cut(s) 124
NdeII GATC 3 cut(s) 517, 531, 538
NgoMIV GCCGGC 2 cut(s) 21, 676
NlaIII CATG 1 cut(s) 555
NlaIV GGNNCC 6 cut(s) 97, 128, 369, 574, 597, 619
NmeAIII GCCGAG 1 cut(s) 165
PaeR7I CTCGAG 1 cut(s) 588
PceI AGGCCT 1 cut(s) 170
PcsI WCGNNNNNNNCGW 6 cut(s) 56, 246, 312, 315, 321, 540
PdiI GCCGGC 2 cut(s) 23, 678
PdmI GAANNNNTTC 1 cut(s) 497
PfeI GAWTC 7 cut(s) 36, 47, 61, 281, 464, 498, 660
PflMI CCANNNNNTGG 1 cut(s) 162
PfoI TCCNGGA 1 cut(s) 122
PkrI GCNGC 6 cut(s) 146, 215, 263, 393, 422, 586
PleI GAGTC 1 cut(s) 626
PluTI GGCGCC 1 cut(s) 99
PpsI GAGTC 1 cut(s) 626
Psp124BI GAGCTC 1 cut(s) 18
Psp6I CCWGG 3 cut(s) 54, 105, 255
PspFI CCCAGC 1 cut(s) 69
PspGI CCWGG 3 cut(s) 54, 105, 255
PspN4I GGNNCC 6 cut(s) 97, 128, 369, 574, 597, 619
PspPI GGNCC 2 cut(s) 52, 596
PspXI VCTCGAGB 1 cut(s) 588
PsuI RGATCY 1 cut(s) 517
SacI GAGCTC 1 cut(s) 18
SatI GCNGC 6 cut(s) 145, 214, 262, 392, 421, 585
Sau3AI GATC 3 cut(s) 517, 531, 538
Sau96I GGNCC 2 cut(s) 52, 596
SchI GAGTC 1 cut(s) 626
ScrFI CCNGG 4 cut(s) 56, 107, 124, 257
SduI GDGCHC 1 cut(s) 18
SfaNI GCATC 1 cut(s) 422
SfoI GGCGCC 1 cut(s) 97
Sfr274I CTCGAG 1 cut(s) 588
SinI GGWCC 2 cut(s) 52, 596
SlaI CTCGAG 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 588
SmoI CTYRAG 1 cut(s) 588
Sse9I AATT 3 cut(s) 7, 100, 275
SseBI AGGCCT 1 cut(s) 170
SspDI GGCGCC 1 cut(s) 95
SspMI CTAG 1 cut(s) 691
SstI GAGCTC 1 cut(s) 18
StuI AGGCCT 1 cut(s) 170
StyD4I CCNGG 4 cut(s) 54, 105, 122, 255
StyI CCWWGG 1 cut(s) 84
TaiI ACGT 1 cut(s) 491
TaqI TCGA 8 cut(s) 279, 342, 380, 416, 543, 589, 638, 663
TasI AATT 3 cut(s) 7, 100, 275
TauI GCSGC 2 cut(s) 264, 394
TfiI GAWTC 7 cut(s) 36, 47, 61, 281, 464, 498, 660
TseI GCWGC 4 cut(s) 144, 213, 420, 584
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 243, 588, 675
Van91I CCANNNNNTGG 1 cut(s) 162
VpaK11BI GGWCC 2 cut(s) 52, 596
XapI RAATTY 1 cut(s) 275
XhoI CTCGAG 1 cut(s) 588
XmnI GAANNNNTTC 1 cut(s) 497
XspI CTAG 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.